LncRNA SNHG15 facilitates the advancement of preeclampsia via the miR-451a/ATF2 axis.

IF 3 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Archives of biochemistry and biophysics Pub Date : 2025-10-01 Epub Date: 2025-08-05 DOI:10.1016/j.abb.2025.110570

Min Wu, Jing Li, Xiaoqian Fu, Xiujing Lu, Lu Xiao, Yachang Zeng

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引用次数: 0

Abstract

Preeclampsia (PE) is a pregnancy complication that poses a major risk to both the maternal and the fetal's safety. By studying the role and mechanism of LncRNA SNHG15 in preeclampsia pathogenesis, this study aimed to better understand the pathophysiology and prevention of PE. Placental samples and hypoxic trophoblast cell line--HTR8/SVneo were analyzed using qPCR to determine the differential expression of LncRNA SNHG15. Nuclear-cytoplasmic fractionation assays confirmed that LncRNA SNHG15 is predominantly localized in the cytoplasm of HTR8/SVneo cells, where it modulates cellular functions including proliferation, migration, and invasion. Using a dual luciferase reporter assay and rescue experiment, functions of miRNA-451a and LncRNA SNHG15 in HTR8/SVneo cells were examined. ATF2 expression levels in the hypoxic cell model and after LncRNA SNHG15 and miR-451a interference were confirmed by qPCR and Western blot. Evidence from this study indicates that LncRNA SNHG15 may be involved in the onset of preeclampsia, suggesting its viability as a novel treatment target.

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LncRNA SNHG15通过miR-451a/ATF2轴促进先兆子痫的进展。

先兆子痫（PE）是一种妊娠并发症，对母体和胎儿的安全构成重大风险。本研究通过研究LncRNA SNHG15在子痫前期发病中的作用及机制，旨在更好地了解PE的病理生理及预防。采用qPCR分析胎盘样品和缺氧滋养细胞系HTR8/SVneo，确定LncRNA SNHG15的差异表达。核细胞质分离实验证实，LncRNA SNHG15主要定位于HTR8/SVneo细胞的细胞质中，在那里它调节细胞功能，包括增殖、迁移和侵袭。采用双荧光素酶报告基因实验和拯救实验，检测miRNA-451a和LncRNA SNHG15在HTR8/SVneo细胞中的功能。通过qPCR和Western blot验证ATF2在缺氧细胞模型和LncRNA SNHG15和miR-451a干扰后的表达水平。本研究证据提示LncRNA SNHG15可能参与子痫前期的发病，提示其作为一种新的治疗靶点的可行性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Archives of biochemistry and biophysics 生物-生化与分子生物学

CiteScore

7.40

自引率

0.00%

发文量

245

审稿时长

26 days

期刊介绍： Archives of Biochemistry and Biophysics publishes quality original articles and reviews in the developing areas of biochemistry and biophysics. Research Areas Include: • Enzyme and protein structure, function, regulation. Folding, turnover, and post-translational processing • Biological oxidations, free radical reactions, redox signaling, oxygenases, P450 reactions • Signal transduction, receptors, membrane transport, intracellular signals. Cellular and integrated metabolism.