Animal genetics最新文献

{"title":"Genome-wide association study of novel feedlot profitability-related traits in Nelore cattle","authors":"Letícia Silva Pereira,&nbsp;Fernando Baldi,&nbsp;Guilherme Rosa,&nbsp;Nedenia Bonvino Stafuzza,&nbsp;Tiago Zanett Albertini,&nbsp;Minos Esperândio Carvalho,&nbsp;Raysildo Barbosa Lobo,&nbsp;Elisa Peripolli,&nbsp;Eduardo da Costa Eifert,&nbsp;Cláudio Ulhôa Magnabosco","doi":"10.1111/age.70018","DOIUrl":"https://doi.org/10.1111/age.70018","url":null,"abstract":"<p>This study aimed to conduct a genome-wide association study to identify genomic regions associated with profitability traits in Nelore beef cattle. The dataset included 3614 phenotypic records of accumulated feedlot profitability (AFP) and profit per 15 kg of liveweight gain (PFT) from animals born between 2020 and 2022, participating in the Nelore Brazilian breeding program from the National Association of Breeders and Researchers. From this total, 2127 animals were genotyped with the Clarifide® Nelore 3.0 SNP panel. After quality control, 2127 genotyped animals and 35 658 SNPs remained in the dataset for analysis. The weighted single-step approach for genome-wide association methodology was used to identify genomic regions associated with AFP and PFT. A single-trait animal model was applied to predict genetic values, and SNP effect solutions were obtained from these values. Genomic windows of 10-SNP sliding windows that explained &gt;0.5% of the additive genetic variance of each trait were selected to investigate potential candidate genes. A total of 83 genes within 21 windows and 268 genes within 52 windows associated with the AFP and PFT were identified, respectively. Several genes related to fertility, feed efficiency, carcass traits, muscle and adipose tissue development and metabolism, and lipid and carbohydrate metabolism were identified, along with genes associated with animal behavior. The results revealed that identifying genomic regions and their respective candidate genes contributes substantially to a better understanding of the genetic mechanisms regarding these novel profitability-related phenotypes in Nelore beef cattle.</p>","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":"56 3","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144289291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A VWF missense variant in Havanese dogs with type 3 von Willebrand disease","authors":"Arly-Camila Armas-Jimenez,&nbsp;Alina Randolph,&nbsp;Leigh Anne Clark,&nbsp;Jacquelyn M. Evans,&nbsp;Marjory B. Brooks,&nbsp;Robert Goggs","doi":"10.1111/age.70021","DOIUrl":"https://doi.org/10.1111/age.70021","url":null,"abstract":"<p>Quantitative and functional defects of von Willebrand Factor (VWF) cause the hereditary bleeding disorder von Willebrand disease (VWD). Three types of VWD exist; type 3 is the most severe and rare, characterized by an almost complete absence of VWF protein. In this study, we investigated the cause of type 3 VWD in a family of purebred Havanese dogs. Pedigree analysis suggested an autosomal recessive mode of inheritance. We performed whole genome sequencing of the parents and relatives of two affected siblings, revealing a novel missense variant in the <i>VWF</i> gene. The variant causes a cysteine to glycine substitution at residue 2571 (NP_001002932.1:p.(Cys2571Gly)) within the VWF C4 domain. This residue is highly conserved across vertebrates and plays a critical role in maintaining the structural integrity of VWF through disulfide bonds. This change probably disrupts the conformation of the C4 domain, leading to VWF concentrations undetectable by enzyme-linked immunosorbent assay in affected dogs. Genotyping confirmed an autosomal recessive mode of inheritance, and the variant was found exclusively within the family. Our findings provide new insights into the genetic basis of VWD and highlight the importance of conserved cysteine residues for VWF stability.</p>","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":"56 3","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144264360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dominant blue eyes in Maine Coon cats: New PAX3 variant and updated phenotypic data","authors":"Marie Abitbol,&nbsp;Ana Cloquell,&nbsp;Adriana Kaczmarska,&nbsp;Karen Holmes,&nbsp;Gesine Lühken,&nbsp;Kristina Macaulay","doi":"10.1111/age.70020","DOIUrl":"https://doi.org/10.1111/age.70020","url":null,"abstract":"<p>Feline minimal white spotting associated with blue eyes, a trait called DBE by breeders for dominant blue eyes, was selected recently to create various lineages of cats and the Celestial breed. Previously, we identified three <i>Paired Box 3</i> (<i>PAX3</i>) gene variants that account for some of the DBE phenotypes in cats, confirming different founding effects. Using a candidate gene approach, we identified a fourth <i>PAX3</i> variant in a purebred Maine Coon lineage segregating for DBE and lacking a previously identified genomic variant. Segregation of the variant was consistent with the dominant inheritance pattern observed in this DBE lineage called the “Agostino line” by breeders. This <i>PAX3</i>:c.160del frameshift variant, located in <i>PAX3</i> exon 2, was predicted to produce a truncated PAX3 protein: PAX3:p.(His54ThrTer108) that would lack 78% of the amino acids of the wildtype protein. All 13 DBE Maine Coon cats from the line were heterozygous for the variant, that was absent in all non-DBE Maine Coon cats from the line and in all control cat genomes (<i>n</i> &gt; 300). We propose that this <i>PAX3</i>:c.160del variant represents the <i>DBE</i>^<i>AGO</i> (<i>Agostino Dominant Blue Eyes</i>) allele in the domestic cat. In addition, we improved the phenotypic description of DBE in Maine Coon cats, highlighting the need to reassess the prevalence of deafness and revealing the existence of latent cats in the previously described DBE^RE (Rociri Elvis Dominant Blue Eyes) Maine Coon lineage.</p>","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":"56 3","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/age.70020","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144197498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Forensic identification of species and subspecies of Canidae family by cross-species PCR and real-time PCR","authors":"Aliaksandra E. Hrebianchuk,&nbsp;Nastassia S. Parfionava,&nbsp;Iosif S. Tsybovsky","doi":"10.1111/age.70019","DOIUrl":"https://doi.org/10.1111/age.70019","url":null,"abstract":"<p>We propose an approach to species identification of four species of the <i>Canidae</i> family, the red fox (<i>Vulpes vulpes</i>), the arctic fox (<i>Vulpes lagopus</i>), the raccoon dog (<i>Nyctereutes procyonoides</i>), and the wolf including the gray wolf (<i>Canis lupus lupus</i>) and the domestic dog (<i>Canis lupus familiaris</i>), based on characteristics of manifestation of microsatellite loci during cross-species amplification. Additionally, we present an approach to DNA identification of the subspecies of <i>Canis lupus</i>, the gray wolf and the domestic dog, based on variations in the copy number of the amylase gene. This comprehensive study of canids living in Belarus makes it possible to reliably differentiate forensic samples by species and subspecies before genetic identification of individuals.</p>","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":"56 3","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144179246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Animal genetics 100 years ago","authors":"Frank W. Nicholas","doi":"10.1111/age.70017","DOIUrl":"https://doi.org/10.1111/age.70017","url":null,"abstract":"<p>One hundred years ago, the first book with the phrase “Animal Genetics” in its title was published. It was written by F.A.E. Crew, then Lecturer in Genetics and foundation Director of the Department of Research in Animal Breeding at the University of Edinburgh. The 352 pages of text provide a most interesting summary of the knowledge of animal genetics at that time. It is impressive to see the extent to which the understanding of genetics had developed in just a couple of decades since the rediscovery of Mendelism. There was, for example, recognition that genes are borne on chromosomes; that XX/XY sex determination provides a very satisfactory explanation for most of the relevant evidence; that sex-linked inheritance has a practical application; that variation in quantitative traits is determined by the combined action of many genes and many non-genetic factors; that inbreeding results in substantial decreases in fecundity and fertility due to homozygosity for undesirable alleles; that crossing between lines or breeds gives rise to hybrid vigour (heterosis); and that many disorders are inherited in a Mendelian fashion, and hence can be controlled by informed breeding. There is, however, no mention of Fisher's 1918 paper nor of Wright's recently published inbreeding coefficient and coefficient of relationship. Crew's book inspired the next generation of geneticists, such as Fred Hutt, who travelled from Canada to Edinburgh to do a PhD with Crew, and who later published his own very influential book with the same title, which was dedicated to Crew.</p>","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":"56 3","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/age.70017","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144074285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of genetic diversity and selection signatures on the Zhashi Brown goat through whole genome sequencing data","authors":"Shuang Li,&nbsp;Hong Lei,&nbsp;Zulfiqar Ahmed,&nbsp;Hongfeng Duan,&nbsp;Jianbo Li,&nbsp;Haobang Li,&nbsp;Chuzhao Lei,&nbsp;Baizhong Zhang,&nbsp;Kangle Yi","doi":"10.1111/age.70016","DOIUrl":"https://doi.org/10.1111/age.70016","url":null,"abstract":"<p>The Zhashi Brown goat is native to Hengyang Municipality in Hunan Province in southern China and boasts a rich history. The goats exhibit exceptional traits, including heat and insect resistance, strong reproductive capabilities and superior meat production. Despite these merits, the currently limited population requires immediate conservation endeavors. In this study, we conducted whole-genome resequencing on 21 Zhashi Brown goats. Additionally, we performed a joint analysis using published whole-genome data from 119 goats, including Chengdu Brown goat, Matou goat, Wuxue goat, Xiangdong Black goat, Qaidam Cashmere goat, Ujumqin Cashmere goat and Shanbei Cashmere goat. The results revealed that the Zhashi Brown goat is genetically more pure than other Southern Chinese goat breeds. Furthermore, the genetic diversity (nucleotide diversity, linkage disequilibrium, runs of homozygosity and inbreeding coefficient) of the Zhashi Brown goat's genome is at a low level among the eight breeds, indicating the need for further conservation. Employing analytical methodologies such as composite likelihood ratio, nucleotide diversity, integrated haplotype score, the fixation index and cross-population extended haplotype homozygosity, we systematically scanned selective signals within the genomic landscape of Zhashi Brown goat. The outcomes underscore strong selection signals associated with genes implicated in immune response, heat tolerance, reproductive performance and meat quality. These findings make a significant contribution to our understanding of the genetics framework associated with adaptive traits in Zhashi Brown goat. Furthermore, this study explores the genetic diversity of the Zhashi Brown goat, which may contribute to the theoretical framework for conserving its genetic resources, while the identified trait-associated variations could inform future strategies to optimize selective breeding programs.</p>","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":"56 3","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143944633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide analysis of genetic diversity and selection signatures in Fuzhou cattle","authors":"Nan Wang,&nbsp;Yushan Li,&nbsp;Xinyi Li,&nbsp;Hao Li,&nbsp;Chenqi Bian,&nbsp;Xinyu Chen,&nbsp;Halima Jafari,&nbsp;Ningbo Chen,&nbsp;Chuzhao Lei","doi":"10.1111/age.70015","DOIUrl":"https://doi.org/10.1111/age.70015","url":null,"abstract":"<p>The Fuzhou cattle breed, native to northeast China, is widely recognized for its adaptability, disease resistance, and docility. Despite being known for these qualities, its population has declined recently, and there is a significant lack of genomic studies on this species. We sequenced 21 samples from a primary breeding farm to determine the genetic structure, diversity, and selection signature to address this. Additionally, we combined 100 published genomic datasets from diverse geographical regions to characterize the genomic variation of Fuzhou cattle. There were 53 752 978 bi-allelic SNPs retained for downstream analysis. In population structure analysis, Fuzhou cattle show a predominantly East Asian taurine ancestry, with strong genetic affinities to Hanwoo and Yanbian cattle. Despite high nucleotide diversity within the Bos taurine lineage, genetic diversity analysis also revealed significant levels of inbreeding in Fuzhou cattle populations, indicating the need for conservation. Utilizing various methods such as <i>θπ</i>, iHS, <i>F</i>_ST, <i>π</i>-ratio, and XP-EHH, we identified genes associated with traits like growth, meat quality, energy metabolism, and immunity. Several genes related to cold adaptation were identified, including <i>PLIN5</i>, <i>PLB1</i>, and <i>CPT2</i>. These findings provide a basis for conservation strategies to safeguard the genetic resources of Fuzhou cattle.</p>","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":"56 3","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143908923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative genomics reveals common diversity and adaptation to harsh environments in the Arabian Peninsula indigenous chickens","authors":"Abdulwahad Assiri,&nbsp;Adriana Vallejo-Trujillo,&nbsp;Mohammed Al-Abri,&nbsp;Hussain Bahbahani,&nbsp;Faisal Almathen,&nbsp;Abulgasim Ahbara,&nbsp;Waleed Al Marzooqi,&nbsp;Abdulfatai Tijjani,&nbsp;Raman Lawal,&nbsp;Olivier Hanotte","doi":"10.1111/age.70014","DOIUrl":"https://doi.org/10.1111/age.70014","url":null,"abstract":"<p>Identifying genomic regions under selection is crucial for comprehending the evolutionary history of the domestic chicken. Arabian Peninsula (AP) indigenous chickens are mostly found outdoors, being reared alongside other livestock for production purposes. These birds show high resilience to extreme temperatures (hot and cold), typical of the desert environment. The selection pressures responsible for unique local adaptations in these birds remain largely unidentified. Here, we aimed to investigate the genome diversity and structure of 15 indigenous chicken populations including 13 populations from the AP (<i>n</i> = 5), Ethiopia (<i>n</i> = 6), and the People's Republic of China (<i>n</i> = 2). We also included two commercial chicken populations, Fayoumi (selected for heat tolerance) and Chantecler (known for its cold tolerance). Principal component (PC) analysis separated all the populations based on their geographic areas of origin. PC1 separates the Ethiopian populations from the Chinese and AP populations, while PC2 separates the AP populations from the Chantecler, and the Ethiopian populations from the Dulong and Chantecler. The genome-wide signatures of analyses identified many candidate regions under positive selection. They include genes that may be associated with thermotolerance. These are involved in energy balance and metabolism (<i>SUGCT</i>, <i>HECW1</i>, <i>MMADHC</i>), cells apoptosis (<i>APP</i>, <i>SRBD1</i>, <i>NTN1</i>, <i>PUF60</i>, <i>SLC26A8</i>, <i>DAP</i>, <i>SUGCT</i>), angiogenesis (<i>RYR2</i>, <i>LDB2</i>, <i>SOX5</i>), skin protection to solar radiation (<i>FZD10</i>, <i>BCO2</i>, <i>WNT5B</i>, <i>COL6A2</i>, <i>SIRT1</i>) as well as growth (<i>NELL1</i>). Our findings suggest that Arabian chicken populations have a distinct gene pool polymorphism in relation to their adaptation to the harsh climatic environments of the AP.</p>","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":"56 3","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/age.70014","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143897153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differences in allele frequencies of personality-related genes in three varieties of Shiba Inu in Japan","authors":"Minori Arahori,&nbsp;Yuki Matsumoto,&nbsp;Noriyoshi Akiyama,&nbsp;Miho Inoue-Murayama","doi":"10.1111/age.70012","DOIUrl":"https://doi.org/10.1111/age.70012","url":null,"abstract":"<p>Shiba Inu is a Japanese dog breed that originally functioned as a hunting dog but is now widely kept as a pet. This study conducted whole genome sequencing on two varieties of Shiba Inu, Mino-Shiba and San'in-Shiba, which are believed to strongly retain the hunting function, and compared them with the common pet Shiba Inu. The results showed that populations of the three varieties formed distinct clusters. We calculated the <i>F</i>_ST values for each site between each of the two varieties and identified genomic regions with significant differences that might affect function. <i>LRRTM4</i> and <i>OXTR</i> found in previous studies on dogs related to personality traits are indicated in this study, and <i>KIF27</i>, associated with Williams syndrome in humans, was also suggested to potentially influence hunting traits. This study highlights the unique genetic lineage of regional Shiba varieties compared to the commonly kept pet Shiba Inu, and provides a foundation for further research into how these genetic differences may affect current personality traits.</p>","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":"56 3","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143871871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A FAM8A1 frameshift variant is associated with REM sleep behavior disorder, urinary retention, and mydriasis in Russian Blue cats","authors":"Kimberley Stee,&nbsp;Mario Van Poucke,&nbsp;Jaume Alomar Huguet,&nbsp;Martí Pumarola Batlle,&nbsp;Kenny Bossens,&nbsp;Ariel Cohen-Solal,&nbsp;Leen Van Brantegem,&nbsp;Kaatje Kromhout,&nbsp;Sofie F. M. Bhatti,&nbsp;Luc Peelman,&nbsp;Ine Cornelis","doi":"10.1111/age.70013","DOIUrl":"https://doi.org/10.1111/age.70013","url":null,"abstract":"<p>REM sleep behavior disorder (RBD) is a disease characterized by the loss of lower motor neuron inhibition responsible for skeletal muscle atonia during REM sleep. It has been reported in humans, dogs and cats, and can be idiopathic or secondary to a neurodegenerative disease. Five young adult Russian Blue cats from two related families were presented for progressively worsening RBD episodes frequently associated with urinary loss. Three of these cats also suffered urinary retention with overflow incontinence between RBD episodes. Neurological examination revealed a large bladder in three cats and a bilateral mydriasis with absent pupillary light reflexes in two cats; further examinations were unremarkable. Treatment attempts were unsatisfactory, with four cats being euthanized. Histopathology of the brain did not reveal any abnormalities. A disease-associated 23-bp deletion in exon 1 of <i>FAM8A1</i> (NC_058372.1:g.11622168_11622190del), introducing a frameshift at codon 162 and a premature stop codon at codon 276 (XM_019831563.3:c.485_507del p.(Gln162Profs*115)), was identified by whole genome sequencing. The variant segregated in the affected families with a recessive mode of inheritance, showed an allele frequency of 1.5% in West-European Russian Blue cats (<i>N</i> = 68) and was not present in 276 cats belonging to 32 other breeds (including the closely related Nebelung breed). The variant FAM8A1 isoform is predicted to affect the assembly and activity of the endoplasmic reticulum-associated protein degradation pathway, which plays an important role in cell homeostasis. RBD and urinary retention syndrome is a hereditary encephalopathy affecting Russian Blue cats. A genetic test now allows diagnosis and prevention of this debilitating disease.</p>","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":"56 3","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143861835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}