Animal genetics最新文献

{"title":"Transcriptome and Functional Analysis Uncover the Role of ADCY5 in Bovine Adipogenesis","authors":"Xuefeng Wei,&nbsp;Xinyue Shan,&nbsp;Lize Yang,&nbsp;Xue Zhao,&nbsp;Yun Ma,&nbsp;Pengpeng Zhang,&nbsp;Yongjie Xu,&nbsp;Ruijie Hao","doi":"10.1002/age.70191","DOIUrl":"10.1002/age.70191","url":null,"abstract":"<div>\u0000 \u0000 <p>Adipose deposition is genetically regulated and acts as a core determinant of meat quality in livestock. Therefore, exploring the gene regulatory mechanisms underlying adipose deposition is essential to advance the research on adipose tissue development and molecular breeding in livestock. To clarify the molecular basis of the superior meat quality of Pinan (PN) cattle, high-throughput RNA sequencing was performed to screen the key genes regulating adipose deposition in PN, with Nanyang (NY) cattle serving as the control (<i>n</i> = 3 per breed). A total of 265 differentially expressed genes (DEGs) were identified in the adipose tissue of PN cattle relative to NY cattle, comprising 135 upregulated and 130 downregulated genes. GO and KEGG enrichment analysis revealed that these DEGs were primarily enriched in lipid binding-related functional categories, particularly lipid antigen binding and exogenous lipid antigen binding, as well as enzymatic functions such as protein xylosyltransferase activity. Meanwhile, RT-qPCR confirmed significant differential expression of <i>WNT16, IGFBP2</i>, <i>PEMT</i>, <i>ADCY5</i>, and <i>IDH3B</i> in adipose tissue between PN and NY cattle, consistent with the RNA-seq data. Moreover, functional validation experiments, including RT-qPCR, CCK-8, EdU, and Oil Red O staining, revealed that RNA interference (RNAi)-mediated <i>ADCY5</i> knockdown markedly promoted adipocyte proliferation, while significantly inhibiting adipocyte differentiation and lipid droplet formation. Collectively, the present study indicates that the identified DEGs are potentially involved in the regulation of bovine adipose tissue development. Notably, <i>ADCY5</i> exhibits a crucial regulatory effect on cattle adipose deposition; however, the precise molecular mechanism remains to be further elucidated in future studies.</p>\u0000 </div>","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":"57 5","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148811794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SINE Insertion in LAMA3 in Dogs With Junctional Epidermolysis Bullosa","authors":"Sarah Kiener,&nbsp;Ronnie Kaufmann,&nbsp;Ori Brenner,&nbsp;Vidhya Jagannathan,&nbsp;Tosso Leeb","doi":"10.1002/age.70190","DOIUrl":"10.1002/age.70190","url":null,"abstract":"<p>Junctional epidermolysis bullosa (JEB) is a hereditary skin disorder caused by defects in proteins responsible for dermal-epidermal adhesion. We investigated the genetic cause of JEB in three related mixed-breed puppies presenting with congenital skin blistering and ulceration. Whole-genome sequencing of one affected dog followed by comparison with 1538 control genomes identified a private candidate variant, XM_038543644.1:c.5690_5691ins240, in <i>LAMA3</i>, a known JEB-associated gene. Visual inspection of the short-read alignments and Sanger sequencing revealed a homozygous 240-bp SINE insertion in exon 45 that had initially been miscalled as a heterozygous short insertion. Fragment length analysis confirmed complete co-segregation of the variant with the disease phenotype within the available family. The SINE insertion is flanked by a 16-bp target site duplication, contains a 45-nt poly(A) tail, and is predicted to remain in-frame, introducing an additional 80 amino acids into the laminin α3 coiled-coil domain without introducing a premature stop codon. Although the molecular consequences were not functionally investigated, the insertion is expected to disrupt normal laminin-332 heterotrimer assembly and secretion. This study expands the spectrum of pathogenic <i>LAMA3</i> variants associated with canine JEB and highlights the importance of visual inspection of short-read sequencing data for the detection and correct interpretation of structurally complex variants such as transposable element insertions.</p>","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":"57 5","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13500218/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148808040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole-Genome Resequencing Reveals Selection Signatures Associated With Lambskin Patterns in Hu Sheep","authors":"Kebede Habtegiorgis Beshah,&nbsp;Jing Xie,&nbsp;Muhammad Tariq,&nbsp;Abdul Quddus,&nbsp;Daxiang Wang,&nbsp;Bingru Zhao,&nbsp;Dagan Mao","doi":"10.1002/age.70186","DOIUrl":"10.1002/age.70186","url":null,"abstract":"<div>\u0000 \u0000 <p>In China, Hu sheep lambskin quality is largely determined by wool curvature, with wavy-wool lambs producing higher-quality pelts than straight-wool lambs. However, the genetic mechanisms underlying wool curvature formation remain poorly understood. To address this knowledge gap, we conducted whole-genome resequencing and transcriptomic analyses to identify candidate genes associated with wool curvature in Hu sheep. Phenotypic records from 3047 Hu lambs identified significant differences between wavy- and straight-wool groups. Twenty representative animals (10 per group) were selected for whole-genome resequencing. Selective sweep analyses based on the fixation index (FST), absolute log₂-transformed nucleotide diversity ratio [|log₂(<i>π</i> ratio)|], and Tajima's D (Taj<i>D</i>) identified 157 genomic windows under positive selection. Four major genomic regions showing strong selective signatures were located on chromosomes 4, 7, 13, and 18. Several biologically relevant candidate genes were identified within these regions, including <i>KRTDAP</i>, <i>FGF2</i>, <i>FGF7</i>, <i>OCLN</i>, <i>MAPK1</i>, <i>SHC4</i>, <i>LAMA2</i>, and <i>COL9A3</i>. Functional enrichment analysis indicated that these genes are primarily associated with extracellular matrix (ECM) organization, focal adhesion, PI3K–Akt, MAPK, and Ras signaling pathways. In addition, RNA sequencing (RNA-seq) data from an independent cohort of eight lambs were integrated with genomic selection signals. This integrative analysis identified 61 overlapping genes, from which 12 high-confidence candidate genes were prioritized, including <i>LUM, COL6A1, SPARC, ITGA11,</i> and <i>DCLK1</i>. These findings highlight genomic regions and candidate genes potentially associated with ECM remodeling and other biological processes relevant to lambskin pattern formation, providing valuable genomic resources for future functional studies and marker-assisted selection in Hu sheep.</p>\u0000 </div>","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":"57 5","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148808117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Multi-Breed Genomic Prediction Models for Carcass and Reproductive Traits in Zebu Beef Cattle","authors":"Gabriel Gubiani,&nbsp;Larissa Temp,&nbsp;Marisol Londoño-Gil,&nbsp;Miller Teodoro,&nbsp;Eduarda Oliveira,&nbsp;Ignacio Aguilar,&nbsp;Matias Bermann,&nbsp;Daniela Lourenco,&nbsp;Angelica S. C. Pereira,&nbsp;Fernando Baldi","doi":"10.1002/age.70188","DOIUrl":"https://doi.org/10.1002/age.70188","url":null,"abstract":"<p>Genomic prediction in beef cattle is particularly challenging in breeds with limited phenotypic and genotypic data. In this context, multi-breed methodologies that integrate information from genetically related populations have emerged as a promising strategy to improve prediction accuracy and calibration under data-scarce conditions. This study evaluated different genomic prediction approaches in a multi-breed population of Zebu cattle, including Nellore, Guzerat, Brahman, and Tabapua, using 653 785 phenotypic records, 190 865 genotypic records, and 3 681 158 pedigree records. The traits analyzed were rib eye area (REA), rump fat thickness (RFT), age at first calving (AFC), and accumulated productivity (ACP). Genomic estimated breeding values were obtained using the single-step GBLUP method under four models: single-breed (G_SB), standard multi-breed (G₀), metafounders (MF), and an adjusted genomic relationship matrix (AGR). Model performance was assessed using the linear regression method, which compares predictions from complete and partial datasets to estimate accuracy, bias, and dispersion. Multi-breed models, particularly MF and AGR, produced higher accuracy than the single-breed approach in several analyses for underrepresented breeds such as Guzerat, Brahman, and Tabapua, especially for carcass and reproductive-related traits. For example, in Guzerat, REA accuracy increased from 0.44 with G_SB to 0.62 with AGR, while in Tabapua, ACP accuracy improved substantially from 0.23 with G_SB to 0.51 with AGR. These results highlight the importance of leveraging genetically related breeds and well-structured reference populations to improve the reliability of genomic predictions in Zebu cattle under limited data conditions.</p>","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":"57 5","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/age.70188","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148784408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MiR-376c-3p Targets STRBP mRNA to Regulate Cashmere Goat Hair Follicle Stem Cell Proliferation and Multipotency by Inhibiting the Wnt/β-Catenin and BMP Signaling Pathways","authors":"Yi Guo,&nbsp;Niu Wang,&nbsp;Xiaojie Hou,&nbsp;Longkang Sun,&nbsp;Hulin He,&nbsp;Xin Wang","doi":"10.1002/age.70185","DOIUrl":"https://doi.org/10.1002/age.70185","url":null,"abstract":"<div>\u0000 \u0000 <p>The quality of cashmere fiber is fundamentally determined by the embryonic morphogenesis of hair follicles, a process highly dependent on the normal function of hair follicle stem cells (HFSCs). MicroRNAs (miRNAs) are key post-transcriptional regulators, but their specific roles in cashmere goat hair follicle development remain unclear. In this study, we identified the differentially expressed miRNA, miR-376c-3p, by analyzing transcriptomic data from embryonic skin (E65, E90, E120) of cashmere goats. Functional assays, including CCK8, crystal violet staining, clonogenic assay, and analysis of proliferation/apoptosis-related proteins (PCNA, CDK4, MKI67, BCL2, CAS3 and CAS9), demonstrated that miR-376c-3p significantly inhibited HFSCs proliferation, induced apoptosis, and blocked the differentiation process, as evidenced by the downregulation of differentiation-associated keratins (KRT4, KRT10, KRT14) and the upregulation of stemness maintenance factors (OCT4, NANOG, SOX9 and KRT15). Mechanistic investigations revealed that miR-376c-3p directly bound to the 3′UTR of <i>STRBP</i> mRNA and inhibited its expression, which was validated by a dual-luciferase reporter assay. Conversely, overexpression of STRBP promoted HFSCs proliferation and differentiation. Further analysis found that STRBP functioned by activating the Wnt/β-catenin and BMP signaling pathways, whereas miR-376c-3p inhibited these same pathways. In summary, this study elucidates a novel regulatory axis: miR-376c-3p influencing the Wnt/β-catenin and BMP signaling cascades in HFSCs by targeting <i>STRBP</i> mRNA, thereby regulating cell fate. This research provides important insights into the molecular mechanisms of miRNA-mediated hair follicle morphogenesis and stem cell biology in cashmere goats.</p>\u0000 </div>","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":"57 5","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148784771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole-Genome Resequencing of 299 Large White Pigs Identifies RASAL2 as a Candidate Gene for Feed Efficiency Traits","authors":"Lin Chen,&nbsp;Bin Yang,&nbsp;Haoran Shi,&nbsp;Junzhuo Deng,&nbsp;Jing Gao,&nbsp;Yang Liu","doi":"10.1002/age.70187","DOIUrl":"https://doi.org/10.1002/age.70187","url":null,"abstract":"<div>\u0000 \u0000 <p>Feed efficiency is a core economic trait in pig breeding, with feed costs accounting for 60%–70% of total production expenses. Deciphering its genetic basis is critical for precise molecular breeding and the development of grain-saving pig breeds. However, most existing studies rely on SNP arrays, which cannot capture insertion–deletion (InDel) variants, and systematic research on modern intensively selected Large White pig populations remains scarce. In this study, we conducted a genome-wide association study (GWAS) for three core traits—feed conversion ratio (FCR), average daily gain (ADG), and average daily feed intake (ADFI)—using whole-genome resequencing data from 299 purebred Large White pigs, with simultaneous detection of genome-wide single nucleotide polymorphisms (SNPs) and InDels. We identified multiple genomic regions significantly associated with feed efficiency traits. An intronic InDel in <i>RASAL2</i> represented the strongest FCR association signal. As a specific negative regulator of the Ras signaling pathway, <i>RASAL2</i> positively regulates preadipocyte differentiation and ameliorates high-fat diet-induced insulin resistance, suggesting it may be associated with feed conversion ratio through energy deposition and metabolic homeostasis. We further annotated 11 additional core candidate genes including <i>TAFA5</i>, <i>TMCC3</i>, <i>BTG1</i>, and <i>PLCB1</i>, which are mainly involved in adipogenesis, energy homeostasis, and skeletal muscle development. These findings enrich genetic resources for porcine feed efficiency research and support molecular marker development for genomic selection in Large White pigs.</p>\u0000 </div>","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":"57 5","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148784407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Approaches to Thoroughbred Horse Identification in Diverse Sample Types Using Multiplexed SNP-PCR Sequencing Panels.","authors":"Jillian Maniego, Caitlin Harding, Pamela Hincks, Jocelyn Habershon-Butcher, Edward Ryder","doi":"10.1002/age.70170","DOIUrl":"10.1002/age.70170","url":null,"abstract":"<p><p>The ability to determine the identity of equine samples would be highly advantageous in cases of ambiguous sample source, as individual-specific controls for gene editing detection, or forensic examination of suspect biological materials in potential doping cases. To this end, a SNP-PCR sequencing-based panel comprising 93 markers in one reaction was designed and investigated for use in Thoroughbred horses. Marker amplification performance and discriminating power were calculated against a population survey of 264 Thoroughbred whole blood samples from routine doping testing. The sensitivity of the SNP panel was tested on a dilution series and on a variety of matrices, including different types of blood specimen, hair and faeces. No genotyping errors due to allelic dropout were detected down to a starting concentration of 0.25 ng/μL in the dilution series, with both EDTA and lithium heparin anticoagulants performing comparably. Forensic 'crime scene' samples, such as blood on a blood tube label, also performed strongly, although others were more variable due to low DNA concentrations. A mock case of 10 'suspects' and one test blood sample was performed blind by the analyst who correctly identified the source of the sample.</p>","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":"57 4","pages":"e70170"},"PeriodicalIF":2.2,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13418657/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148618094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Functional SNP in the Promoter Region of Ovine IDO2 Associated With Melatonin Levels via Affecting Transcription Activity by CLOCK.","authors":"Xunping Jiang, Shaxuan Chi, Xiaodong Wang, Mengya Chen, Zhuoying Tie, Huiguo Yang, Chaoli Wang, Guiqiong Liu","doi":"10.1002/age.70173","DOIUrl":"10.1002/age.70173","url":null,"abstract":"<p><p>Sheep seasonal reproduction is regulated by photoperiod via melatonin. Indoleamine 2,3-dioxygenase 2 (IDO2), an enzyme involved in tryptophan metabolism through the kynurenine pathway and belonging to the IDO family, may contribute to melatonin homeostasis, but its role in reproductive seasonality remains unclear. In this study, genome-wide selection analyses combining Z(F_ST) and XP-EHH between seasonal and non-seasonal sheep identified a selection signature encompassing the IDO2 region and revealed g.-809T>C as a functional candidate variant within this region. Experiments employing electrophoretic mobility shift assay (EMSA) and luciferase assays revealed that this site lies within the CLOCK transcription factor binding domain. The -809C variant reduced promoter activity compared to -809T (p < 0.05). Sheep with the CC genotype had higher plasma melatonin levels than TC/TT counterparts (p < 0.05). These findings suggest that the g.-809C allele decreases IDO2 transcription by reducing CLOCK binding affinity, thereby potentially affecting IDO2-associated tryptophan/melatonin metabolism and increasing melatonin levels, which may contribute to photoperiodic responses in sheep. This IDO2 variation provides new insights into the genetic regulation of melatonin-mediated reproductive seasonality and represents a potential marker for genetic improvement of reproductive seasonal traits in sheep.</p>","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":"57 4","pages":"e70173"},"PeriodicalIF":2.2,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13429496/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148663515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Species Determination Within the Felidae Family Using mtDNA Minibarcoding.","authors":"Kristýna Hebenstreitová, Karolina Mahlerová, Lenka Vaňková, Daniel Vaněk","doi":"10.1002/age.70181","DOIUrl":"10.1002/age.70181","url":null,"abstract":"<p><p>Wildlife forensic analysis frequently deals with highly degraded DNA samples, including those from tanned hides, processed products of traditional Chinese medicine, and specimens stored in preservation fluids. These samples present significant challenges for traditional DNA barcoding due to the difficulty of amplifying sufficiently long DNA fragments. To overcome this limitation, we designed five primer pairs to amplify short mitochondrial DNA (mtDNA) fragments (~100 base pairs). When aligned collectively, these fragments span approximately 500 bp of the cytochrome C oxidase subunit I (COI) gene, which is one of the most common species-barcoding targets. We subjected the primers to a comprehensive validation process, including in silico analysis and experimental verification using various non-degraded and degraded samples of animal tissues. Here, we demonstrate the efficacy and reliability of our DNA minibarcoding method. By addressing the limitations of traditional DNA barcoding, this method improves the accuracy and success of species identification, thereby supporting wildlife conservation, management, and forensic investigations.</p>","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":"57 4","pages":"e70181"},"PeriodicalIF":2.2,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13462279/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148711102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Frameshift Variant in SPAG1 Likely Causes Primary Ciliary Dyskinesia in Cocker Spaniels in Australia.","authors":"S E Mead, L E Hambrook, R Suryadinata, P Robinson, C M Wade","doi":"10.1002/age.70183","DOIUrl":"10.1002/age.70183","url":null,"abstract":"<p><p>Primary ciliary dyskinesia (PCD) is a clinical syndrome that in dogs primarily manifests as chronic respiratory disease associated with cilial malfunction. The current study employs whole-genome sequencing and a candidate gene approach to uncover the genetic basis of PCD in three Cocker Spaniel siblings following diagnosis of their respiratory cilia by scanning electron microscopy and high-speed video microscopy. Absence of the disorder in the parents suggested autosomal recessive inheritance. A 29 bp frameshift insertion in the eleventh exon of the candidate gene sperm-associated antigen 1 (SPAG1) [NC_049234.1:g.2213788_2213789insGGCGGCGGCAAGCGGCCGGAGAGGGGCGC] was identified as likely causative for PCD in this family. The 29 bp frameshift variant was unobserved in a public variant call file including 1987 dogs from the Dog10K resource however an in-frame insertion was sometimes observed at the same locus. A Cocker Spaniel with similar symptoms from a different family tested negative for the identified variant suggesting that there are multiple causes for the condition in Cocker Spaniels.</p>","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":"57 4","pages":"e70183"},"PeriodicalIF":2.2,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13456965/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148705066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}