Animal genetics最新文献

{"title":"Genome-wide association studies for loin muscle area, loin muscle depth and backfat thickness in DLY pigs","authors":"Xiang Zhao,&nbsp;Yibin Qiu,&nbsp;Fanming Meng,&nbsp;Zhanwei Zhuang,&nbsp;Donglin Ruan,&nbsp;Jie Wu,&nbsp;Fucai Ma,&nbsp;Enqin Zheng,&nbsp;Gengyuan Cai,&nbsp;Jie Yang,&nbsp;Ming Yang,&nbsp;Zhenfang Wu","doi":"10.1111/age.13386","DOIUrl":"10.1111/age.13386","url":null,"abstract":"<p>This study aimed at identifying genes associated with loin muscle area (LMA), loin muscle depth (LMD) and backfat thickness (BFT). We performed single-trait and multi-trait genome-wide association studies (GWASs) after genotyping 685 Duroc × (Landrace × Yorkshire) (DLY) pigs using the Geneseek Porcine 50K SNP chip. In the single-trait GWASs, we identified two, eight and two significant SNPs associated with LMA, LMD and BFT, respectively, and searched genes within the 1 Mb region near the significant SNPs with relevant functions as candidate genes. Consequently, we identified one (<i>DOCK5</i>), three (<i>PID1</i>, <i>PITX2</i>, <i>ELOVL6</i>) and three (<i>CCR1</i>, <i>PARP14</i>, <i>CASR</i>) promising candidate genes for LMA, LMD and BFT, respectively. Moreover, the multi-trait GWAS identified four significant SNPs associated with the three traits. In conclusion, the GWAS analysis of LMA, LMD and BFT in a DLY pig population identified several associated SNPs and candidate genes, further deepening our understanding of the genetic basis of these traits, and they may be useful for marker-assisted selection to improve the three traits in DLY pigs.</p>","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138680609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole-genome resequencing reveals candidate genes associated with milk production trait in Guanzhong dairy goats","authors":"Jie Ni,&nbsp;Ming Xian,&nbsp;Yijie Ren,&nbsp;Lina Yang,&nbsp;Yu Li,&nbsp;Songmao Guo,&nbsp;Benkang Ran,&nbsp;Jianhong Hu","doi":"10.1111/age.13380","DOIUrl":"10.1111/age.13380","url":null,"abstract":"<p>Milk production is one of the most important economic utility of goats. Guanzhong dairy goat is a local dairy goat in Shaanxi Province of China and has high milk yield and quality. However, there are relatively few studies on molecular markers of milk production traits in Guanzhong dairy goats. In this study, we sequenced the whole genomes of 20 Guanzhong dairy goats, 10 of which had high milk yield (HM) and 10 of which had low milk yield (LM). We detected candidate signatures of selection in HM goats using <i>F</i>_st and <i>π</i>-ratio statistics and identified several candidate genes including <i>ANPEP</i>, <i>ADRA1A</i> and <i>PRKG1</i> associated with milk production. Our results provide the basis for molecular breeding of milk production traits in Guanzhong dairy goats.</p>","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138795370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated analysis of whole genome and transcriptome sequencing uncovers genetic differences between Zi goose and Xianghai flying goose","authors":"Hongyu Ni,&nbsp;Yonghong Zhang,&nbsp;Yuwei Yang,&nbsp;Yijing Yin,&nbsp;Jing Ren,&nbsp;Qingxing Xiao,&nbsp;Puze Zhao,&nbsp;Xiaoqing Hong,&nbsp;Ziyi Zhang,&nbsp;Benhai Cui,&nbsp;Hao Sun,&nbsp;Xueqi Sun,&nbsp;Yumei Li","doi":"10.1111/age.13388","DOIUrl":"10.1111/age.13388","url":null,"abstract":"<p>Zi goose is a famous indigenous breed originating from northeast China with high annual egg production. Xianghai flying goose is a composite breed and is bred by crosses of the wild swan goose and the Zi goose. Our previous study revealed significant differences in muscle fiber characteristics between the two populations. Here, we aimed to reveal the underlying genetic basis of the above phenotype differences through whole-genome and transcriptome analysis. A total of 20 blood samples (10 Zi geese and 10 Xianghai flying geese) were used for whole genome sequencing, and eight breast muscle tissue samples (four Zi geese and four Xianghai flying geese) were used for RNA sequencing. Using the <i>F</i>_ST and XP-EHH analysis, some highly differentiated genome regions annotated with egg production (<i>RORB</i>, <i>WNT4</i>, <i>BMPR1B</i>) and breast muscle development (<i>WNT7B</i>) between the two populations were detected. RNA-sequencing analysis revealed differentially expressed genes related to muscle development (<i>IGF1</i>, <i>PAX7</i>). Moreover, several genes were detected by both genome and transcriptome analysis, and some of them were reported to be associated with muscle growth (<i>SLIT2</i>, <i>PREX1</i>) and intramuscular fat (<i>COL6A1</i>). These findings will help researchers better understand the genetic basis related to egg production and muscle development in geese.</p>","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138575774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Npc1 gene mutation abnormally activates the classical Wnt signalling pathway in mouse kidneys and promotes renal fibrosis","authors":"Lihong Guan,&nbsp;Zisen Jia,&nbsp;Keli Xu,&nbsp;Minlin Yang,&nbsp;Xiaoying Li,&nbsp;Liang Qiao,&nbsp;Yanli Liu,&nbsp;Juntang Lin","doi":"10.1111/age.13381","DOIUrl":"10.1111/age.13381","url":null,"abstract":"<p>Niemann–Pick disease type C1 (NPC1) is a lysosomal lipid storage disease caused by <i>NPC1</i> gene mutation. Our previous study found that, compared with wild-type (<i>Npc1</i>^<i>+/+</i>) mice, the renal volume and weight of <i>Npc1</i> gene mutant (<i>Npc1</i>^{<i>−/−</i>}) mice were significantly reduced. We speculate that <i>Npc1</i> gene mutations may affect the basic structure of the kidneys of <i>Npc1</i>^{<i>−/−</i>} mice, and thus affect their function. Therefore, we randomly selected postnatal Day 28 (P28) and P56 <i>Npc1</i>^<i>+/+</i> and <i>Npc1</i>^{<i>−/−</i>} mice, and observed the renal structure and pathological changes by haematoxylin–eosin staining. The level of renal fibrosis was detected by immunofluorescence histochemical techniques, and western blotting was used to detect the expression levels of apoptosis-related proteins and canonical Wnt signalling pathway related proteins. The results showed that compared with <i>Npc1</i>^<i>+/+</i> mice, the kidneys of P28 and P56 <i>Npc1</i>^{<i>−/−</i>} mice underwent apoptosis and fibrosis; furthermore, there were obvious vacuoles in the cytoplasm of renal tubular epithelial cells of P56 <i>Npc1</i>^{<i>−/−</i>} mice, the cell bodies were loose and foam-like, and the canonical Wnt signalling pathway was abnormally activated. These results showed that <i>Npc1</i> gene mutation can cause pathological changes in the kidneys of mice. As age increased, vacuoles developed in the cytoplasm of renal tubular epithelial cells, and apoptosis of renal cells, abnormal activation of the Wnt signalling pathway, and promotion of renal fibrosis increased.</p>","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138579530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic basis of ear length in sheep breeds sampled across the region from the Middle East to the Alps","authors":"Jürgen Klawatsch,&nbsp;Dimitris Papachristou,&nbsp;Panagiota Koutsouli,&nbsp;Maulik Upadhyay,&nbsp;Doris Seichter,&nbsp;Ingolf Russ,&nbsp;Boro Mioč,&nbsp;Mojca Simčič,&nbsp;Iosif Bizelis,&nbsp;Ivica Medugorac","doi":"10.1111/age.13387","DOIUrl":"10.1111/age.13387","url":null,"abstract":"<p>Ear length in sheep (<i>Ovis aries</i>) shows a wide range of natural variation, from the absence of an outer ear structure (<i>anotia</i>), to small outer ears (<i>microtia</i>), to regular ear length. Up until now, the underlying genetics of this phenotype has been studied in four sheep breeds from China, Jordan and Italy. These studies revealed a broad range of genes significantly associated with ear length, potentially indicating genetic heterogeneity across breeds or geographic regions. In the current study, we performed genome-wide SNP genotyping and haplotype-based mapping, in a population of 340 individuals, to identify loci influencing ear length variation in additional sheep breeds from Slovenia, Croatia, Cyprus and Greece. Additionally, two previously described candidate variants were also genotyped in our mapping population. The mapping model without candidate variant genotypes revealed only one genome-wide significant signal, which was located next to <i>HMX1</i> on OAR6. This region was previously described as being associated with ear length variation in the Altay and Awassi sheep breeds. The mapping model including the candidate duplication genotype near <i>HMX1</i> as a fixed effect explained the phenotypic variance on OAR6 and revealed an additional genome-wide significant locus on OAR13 associated with ear length. Our results, combined with published evidence, suggest that a duplication in the evolutionarily conserved region near <i>HMX1</i> is the major regulator of ear length in sheep breeds descended from a larger region from Central Asia, to the Middle East, Cyprus, Greece and to the Alps. This distribution suggests an ancient origin of the derived allele.</p>","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/age.13387","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138560815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide association study of growth traits and validation of key mutations (MSTN c.C861T) associated with the muscle mass of meat pigeons","authors":"Haobin Hou,&nbsp;Xiaoliang Wang,&nbsp;Xin Li,&nbsp;Xia Cai,&nbsp;Yingying Tu,&nbsp;Changsuo Yang,&nbsp;Junfeng Yao","doi":"10.1111/age.13382","DOIUrl":"10.1111/age.13382","url":null,"abstract":"<p>Selective breeding of meat pigeons is primarily based on growth traits, especially muscle mass (MM). Identification of functional genes and molecular markers of growth and slaughter traits through a genome-wide association study (GWAS) will help to elucidate the underlying molecular mechanisms and provide a theoretical basis for the selective breeding of meat pigeons. The phenotypic data of body weight (BW) and body size (BS) of 556 meat pigeons at 52 and 80 weeks of age were collected. In total, 160 434 high-quality single nucleotide polymorphism sites were obtained by restriction site-associated DNA sequencing. The GWAS analysis revealed that <i>MSTN</i>, <i>IGF2BP3</i> and <i>NCAPG</i>/<i>LCORL</i> were important candidate genes affecting the growth traits of meat pigeons. <i>IGF2BP3</i> and <i>NCAPG</i>/<i>LCORL</i> were highly correlated to BW and BS, which are related to overall growth and development, while <i>MSTN</i> was associated with pectoral thickness and BW. Phenotypic association validation with the use of two meat pigeon populations found that the <i>MSTN</i> mutation c.C861T determines the MM. These results provide new insights into the genetic mechanisms underlying phenotypic variations of growth traits and MM in commercial meat pigeons. The identified markers and genes provide a theoretical basis for the selective breeding of meat pigeons.</p>","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138561091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide analyses based on a novel donkey 40K liquid chip reveal the gene responsible for coat color diversity in Chinese Dezhou donkey","authors":"Shuqin Liu,&nbsp;Jiangtian Su,&nbsp;Qiwen Yang,&nbsp;Minhao Sun,&nbsp;Zhaofei Wang,&nbsp;Jie Yu,&nbsp;Halima Jafari,&nbsp;Chuzhao Lei,&nbsp;Yujiang Sun,&nbsp;Ruihua Dang","doi":"10.1111/age.13379","DOIUrl":"10.1111/age.13379","url":null,"abstract":"<p>Dezhou donkey is one of the representative local breeds in China, which is mainly divided into two strains: Sanfen and Wutou. There are obvious differences in coat color between the two strains. The former shows light points around the eyes, around the muzzle and under the belly, while the latter is completely solid black. In this study, genome-wide association analysis was performed for the differences in coat color traits between the Sanfen (<i>n</i> = 97) and Wutou (<i>n</i> = 108) strains using a novel donkey 40K liquid chip developed based on GenoBaits technology, to identify genomic regions and causal genes that could explain this variation. We also used <i>F</i>_ST and The cross-population composite likelihood ratio test (XPCLR) analyses to explore selected regions related to coat color differences. We identified one significant region on chromosome 15, with the most significant SNP located within the agouti signaling protein (<i>ASIP</i>) gene. At the same time, both <i>F</i>_ST and XPCLR methods detected the same selected region on chromosome 15, and <i>ASIP</i> was the gene with the strongest signal. <i>ASIP</i> and melanocortin 1 receptor (<i>MC1R</i>) control the ratio of eumelanin to pheomelanin through their protein activity. They are deeply involved in the process of melanosome organation and melanogenesis, thus affecting mammals’ coat color variation. We used a range of genome-wide approach to identify the genetic basis of coat color variation in Dezhou donkeys. The results provide a supplement to the color variation study in Chinese donkeys at the genome-wide level, and preliminarily verified the reliability of the Molbreeding Donkey No. 1 40K liquid chip.</p>","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138294486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic diversity patterns in farmed rainbow trout (Oncorhynchus mykiss) populations using genome-wide SNP and haplotype data","authors":"Alessio Longo,&nbsp;Khrystyna Kurta,&nbsp;Tytti Vanhala,&nbsp;Henrik Jeuthe,&nbsp;Dirk-Jan de Koning,&nbsp;Christos Palaiokostas","doi":"10.1111/age.13378","DOIUrl":"10.1111/age.13378","url":null,"abstract":"<p>Rainbow trout is one of the most popular aquaculture species worldwide, with a long history of domestication. However, limited information exists about the genetic diversity of farmed rainbow trout populations globally, with most available reports relying on low-throughput genotyping technologies. Notably, no information exists about the genetic diversity status of farmed rainbow trout in Sweden. Double-digest restriction-site-associated DNA sequencing was performed on more than 500 broodfish from two leading producers in Sweden and from the country's national breeding program. Following the detection of single nucleotide polymorphisms (SNPs), genetic diversity was studied by using either individual SNPs (<i>n</i> = 8680; one SNP retained per 300 bp sequence reads) or through SNP haplotypes (<i>n</i> = 20 558; all SNPs retained in 300 bp sequence reads). Similar amounts of genetic diversity were found amongst the three populations when individual SNPs were used. Furthermore, principal component analysis and discriminant analysis of principal components suggested two genetic clusters with the two industry populations grouped together. Genetic differentiation based on the <i>F</i>_ST fixation index was ~0.01 between the industry populations and ~0.05 when those were compared with the breeding program. Preliminary estimates of effective population size (<i>N</i>_e) and inbreeding (based on runs of homozygosity; <i>F</i>_ROH) were similar amongst the three populations (<i>N</i>_e ≈ 50–80; median <i>F</i>_ROH ≈ 0.11). Finally, the haplotype-based analysis suggested that animals from the breeding program had higher shared coancestry levels than those from the other two populations. Overall, our study provides novel insights into the genetic diversity and structure of Sweden's three main farmed rainbow trout populations, which could guide their future management.</p>","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/age.13378","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138294485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relevance of pharmacogenetics and pharmacogenomics in veterinary clinical practice: A review","authors":"Ayushi Vaidhya,&nbsp;Kanika Ghildiyal,&nbsp;Divya Rajawat,&nbsp;Sonali Sonejita Nayak,&nbsp;Subhashree Parida,&nbsp;Manjit Panigrahi","doi":"10.1111/age.13376","DOIUrl":"10.1111/age.13376","url":null,"abstract":"<p>The recent advances in high-throughput next-generation sequencing technologies have heralded the arrival of the Big Data era. As a result, the use of pharmacogenetics in drug discovery and individualized drug therapy has transformed the field of precision medicine. This paradigm shift in drug development programs has effectively reshaped the old drug development practices, which were primarily concerned with the physiological status of patients for drug development. Pharmacogenomics bridges the gap between pharmacodynamics and pharmacokinetics, advancing current diagnostic and treatment strategies and enabling personalized and targeted drug therapy. The primary goals of pharmacogenetic studies are to improve drug efficacy and minimize toxicities, to identify novel drug targets, to estimate drug dosage for personalized medicine, and to incorporate it as a routine diagnostic for disease susceptibility. Although pharmacogenetics has numerous applications in individualized drug therapy and drug development, it is in its infancy in veterinary medicine. The objective of this review is to present an overview of historical landmarks, current developments in various animal species, challenges and future perspectives of genomics in drug development and dosage optimization for individualized medicine in veterinary subjects.</p>","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/age.13376","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138290158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of genomic regions associated with differences in fleece type in Huacaya and Suri alpacas (Vicugna pacos)","authors":"K. Tan,&nbsp;O. O. Adeniyi,&nbsp;A. Letko,&nbsp;G. RuddGarces,&nbsp;E. Manz,&nbsp;H. Wagner,&nbsp;P. Zanolari,&nbsp;C. Drögemüller,&nbsp;G. Lühken","doi":"10.1111/age.13377","DOIUrl":"10.1111/age.13377","url":null,"abstract":"<p>The difference in fleece type is the distinguishing trait between the two types of alpacas (<i>Vicugna pacos</i>), Huacaya and Suri. The Suri fleece type has been found to be inherited dominantly over the Huacaya type, resulting in offspring with the Suri phenotype. The aim of our study was to map genomic regions associated with the two different fleece types. In this study, 91 alpacas (54 Huacayas and 37 Suris) from Germany and Switzerland were genotyped using the 76k alpaca SNP array. Only 59k chromosome-localised markers map to the alpaca reference assembly VicPac3.1, and after quality control 49 866 SNPs, were retained for population structure assessment and to conduct a genome-wide association study. Both principal component and neighbour-joining tree analysis showed that the two fleece-type cohorts overlapped rather than forming two distinct clusters. Genome-wide significantly associated markers were observed in the scaffold region of chromosome 16 (NW_021964192.1), which contains a cluster of keratin genes. A haplotype predominantly found in Suri alpacas has been identified which supports dominant inheritance. Variant filtering of nine whole-genome sequenced alpacas from both fleece types in the critical interval of 0.4 Mb did not reveal perfect segregation of either fleece type for specific variants. To our knowledge, this is the first study to use the recently developed species-specific SNP array to identify genomic regions associated with differences in fleece type in alpacas. There are still some limitations, such as the preliminary status of the reference assembly and the incomplete annotation of the alpaca genome.</p>","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/age.13377","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138175382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}