Animal genetics最新文献

{"title":"A novel QTL region for pH and meat color in Duroc pigs","authors":"Eduard Molinero,&nbsp;Ramona N. Pena,&nbsp;Joan Estany,&nbsp;Roger Ros-Freixedes","doi":"10.1111/age.13426","DOIUrl":"10.1111/age.13426","url":null,"abstract":"<p>One of the most important processes that occur during the transformation of muscle to meat is the pH decline as a consequence of the post-mortem metabolism of muscle tissue. Abnormal pH declines lead to pork defects such as pale, soft, and exudative meat. There is genetic variance for ultimate pH and the role of some genes on this phenotype is well established. After conducting a genome-wide association study on ultimate pH using 526 purebred Duroc pigs, we identified associated regions on <i>Sus scrofa</i> chromosomes (SSC) 3, 8, and 15. Functional candidate genes in these regions included <i>PRKAG3</i> and <i>PHKG1</i>. The SSC8 region, at 71.6 Mb, was novel and, although no candidate causative gene could be identified, it may have regulatory effects. Subsequent analysis on 828 pigs from the same population confirmed the impact of the three associated regions on pH and meat color. We detected no interaction between the three regions. Further investigations are necessary to unravel the functional significance of the novel genomic region at SSC8. These variants could be used as markers in marker-assisted selection for improving meat quality.</p>","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":"55 3","pages":"465-470"},"PeriodicalIF":2.4,"publicationDate":"2024-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/age.13426","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140580792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heterozygous ASPRV1 frameshift variant in a Pembroke Welsh Corgi with ichthyosis","authors":"Sarah Kiener,&nbsp;Susanne Åhman,&nbsp;Robert Cikota,&nbsp;Vidhya Jagannathan,&nbsp;Sohvi Blatter,&nbsp;Iva Cvitas,&nbsp;Sara Soto,&nbsp;Tosso Leeb","doi":"10.1111/age.13423","DOIUrl":"10.1111/age.13423","url":null,"abstract":"&lt;p&gt;Ichthyoses comprise a large, heterogeneous group of inherited cornification disorders. They are characterized by generalized scaly and hyperkeratotic skin (Oji et al., &lt;span&gt;2010&lt;/span&gt;). In humans, over 69 genes have been associated with different forms of ichthyosis, grouped into non-syndromic ichthyoses with the phenotypic expression of the disorder only seen in the skin, and syndromic ichthyoses that show additional organ involvement (Gutiérrez-Cerrajero et al., &lt;span&gt;2023&lt;/span&gt;; Uitto et al., &lt;span&gt;2020&lt;/span&gt;). Further subdivision into epidermolytic and non-epidermolytic ichthyoses is based on the presence or absence of light microscopic findings of vacuoles and lysis of keratinocytes (Mauldin, &lt;span&gt;2013&lt;/span&gt;). In dogs, several breed-specific ichthyoses have been described, and, to date, causal variants in nine different genes have been identified (Affolter et al., &lt;span&gt;2022&lt;/span&gt;; Bauer et al., &lt;span&gt;2017&lt;/span&gt;; Briand et al., &lt;span&gt;2019&lt;/span&gt;; Casal et al., &lt;span&gt;2017&lt;/span&gt;; Credille et al., &lt;span&gt;2005&lt;/span&gt;, &lt;span&gt;2009&lt;/span&gt;; Grall et al., &lt;span&gt;2012&lt;/span&gt;; Kiener et al., &lt;span&gt;2022&lt;/span&gt;; Kiener, Åhman, et al., &lt;span&gt;2023&lt;/span&gt;; Kiener, Castilla, et al., &lt;span&gt;2023&lt;/span&gt;; Metzger et al., &lt;span&gt;2015&lt;/span&gt;). These genes are mainly involved in the biosynthesis, metabolism, and transport of lipids required for skin barrier function or the intracellular protein network responsible for the integrity of skin structure (Gutiérrez-Cerrajero et al., &lt;span&gt;2023&lt;/span&gt;).&lt;/p&gt;&lt;p&gt;A 6-month-old Pembroke Welsh Corgi was presented with non-pruritic severe scaling (large 5–10-mm scales; Figure 1a), hyperkeratotic paw pads (Figure 1b), and fish-skin like flakes and erythema in friction areas (Figure 1c), present since shortly after birth. Histological examination of biopsies taken from haired skin and paw pads showed prominent compact to lamellar orthokeratotic hyperkeratosis (Figure 1d). The observed changes were compatible with non-epidermolytic ichthyosis.&lt;/p&gt;&lt;p&gt;We performed Illumina short-read whole-genome sequencing at 26× coverage on genomic DNA isolated from leukocytes to investigate potential causal genetic variants. The data were processed as previously described (Jagannathan et al., &lt;span&gt;2019&lt;/span&gt;) with respect to the genome reference assembly UU_Cfam_GSD_1.0. Subsequent comparison of the whole-genome sequencing data of the affected dog to 960 genetically diverse canine genomes (Table S1) revealed 76 heterozygous and eight homozygous protein-changing private variants (Table S2). Among them was a heterozygous two base-pair deletion variant in the ichthyosis candidate gene &lt;i&gt;ASPRV1&lt;/i&gt;, XM_038551592.1:c.594_595del or Chr10:NC_049231.1:69888722_69888723del, leading to a frameshift and altering 48% of the wildtype protein sequence, XP_038407520.1:p.(Leu199Argfs*342). The predicted mutant protein contains 539 compared to 381 amino acids in the wildtype protein. &lt;i&gt;ASPRV1&lt;/i&gt; encodes the retroviral-like aspartic protease 1, which","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":"55 3","pages":"490-492"},"PeriodicalIF":2.4,"publicationDate":"2024-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/age.13423","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140317717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of polymorphic markers for germplasm conservation of three precious Chinese palace goldfish using whole-genome sequencing","authors":"Yuwei Huang,&nbsp;Aiying Cao,&nbsp;Beiyuan Zhang,&nbsp;Sen Li,&nbsp;Chuan He,&nbsp;Jian Gao,&nbsp;Xiaojuan Cao","doi":"10.1111/age.13419","DOIUrl":"10.1111/age.13419","url":null,"abstract":"<p>China was the first country in the world to breed goldfish and has generated many unique goldfish varieties, including the most aristocratic Chinese palace goldfish. Due to the lack of scientific research on Chinese palace goldfish, their selection and breeding are mainly carried out through traditional hybridization, leading to serious inbreeding and the degradation of germplasm resources. To this end, whole-genome resequencing was performed to understand the genetic variation among three different varieties (eggpompons, goosehead, and tigerhead) from nine core conserved populations in China. A total of 15 polymorphic SSRs were developed for population genetics, and all tested populations were considered moderately polymorphic with an average polymorphism information content value of 0.4943. Genetic diversity in different varieties showed that all conserved populations were well protected with the potential for continued exploitation. This study provides reliable molecular tools and a basis for designing conservation and management programs in Chinese palace goldfish.</p>","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":"55 3","pages":"484-489"},"PeriodicalIF":2.4,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140157410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic characterization of dryland adaptation in endangered Anxi cattle in China","authors":"Yang Lyu,&nbsp;Tingting Yao,&nbsp;Zhefu Chen,&nbsp;Ruiyao Huangfu,&nbsp;Haijian Cheng,&nbsp;Weidong Ma,&nbsp;Xingshan Qi,&nbsp;Fuqiang Li,&nbsp;Ningbo Chen,&nbsp;Chuzhao Lei","doi":"10.1111/age.13416","DOIUrl":"10.1111/age.13416","url":null,"abstract":"<p>Local species exhibit distinctive indigenous characteristics while showing unique productive and phenotypic traits. However, the advent of commercialization has posed a substantial threat to the survival of indigenous species. Anxi cattle, an endangered native breed in China, have evolved unique growth and reproductive characteristics in extreme desert and semidesert ecosystems. In this study, we conducted a genomic comparison of 10 Anxi cattle genomes with those of five other global populations/breeds to assess genetic diversity and identify candidate genomic regions in Anxi cattle. Population structure and genetic diversity analyses revealed that Anxi cattle are part of the East Asian cattle clade, exhibiting higher genetic diversity than commercial breeds. Through selective sweep analysis, we identified specific genetic variations linked to the environmental adaptability of Anxi cattle. Notably, we identified several candidate genes, including <i>CERS3</i> involved in regulating skin permeability and antimicrobial functions, <i>RBFOX2</i> associated with cardiac development, <i>SLC16A7</i> participated in the regulation of pancreatic endocrine function, and <i>SPATA3</i> related to reproduction. Our findings revealed the distinctive genomic features of Anxi cattle in dryland environments, provided invaluable insights for further research and breed preservation, and had important significance for enriching the domestic cattle breeding gene bank.</p>","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":"55 3","pages":"352-361"},"PeriodicalIF":2.4,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140020811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the effectiveness of single nucleotide polymorphisms compared to microsatellite markers for parentage verification in Moroccan horses","authors":"O. Aminou,&nbsp;B. Badaoui,&nbsp;M. Machmoum,&nbsp;M. Piro","doi":"10.1111/age.13408","DOIUrl":"10.1111/age.13408","url":null,"abstract":"<p>The International Society for Animal Genetics (ISAG) currently advocates for a transition towards single nucleotide polymorphism (SNP) markers as a potential alternative for equine parentage verification. To ascertain the efficacy of this transition, it is imperative to evaluate the performance of parentage testing using SNPs in juxtaposition with short tandem repeats (STRs). As per ISAG's recommendation, we used an equine genotyping-by-sequencing panel with 144 SNPs for this purpose. Equine parentage is currently realized using 16 microsatellites (STRs) excluding the LEX3 marker. In this study, 1074 horses were genotyped using the 144 SNPs panel, including 432 foals, 414 mares, and 228 stallions, from five different breeds: 293 Arabians, 167 Barbs, 189 Thoroughbreds, 73 Anglo-Arabians, and 352 Arabian-Barbs. As a result, two SNPs markers were eliminated from the panel system due to inconsistent amplification across all examined individuals leaving 142 SNPs markers for analysis. A comparative analysis between SNPs and STRs markers revealed that the mean expected heterozygosity was 0.457 for SNPs and 0.76 for STRs, while the mean observed heterozygosity stood at 0.472 for SNPs and 0.72 for STRs. Furthermore, the probability of identity was calculated to be 5.722 × 10⁻⁵⁷ for SNPs and 1.25 × 10⁻¹⁵ for STRs markers. In alignment with the Hardy–Weinberg equilibrium in polyploids test, 110 out of the total SNPs were consistent with the Hardy–Weinberg equilibrium in polyploids test (<i>p</i> &gt; 0.05). Employing both SNPs and STRs markers, the mean polymorphic information content was discerned to be 0.351 for SNPs and 0.72 for STRs. The cumulative exclusion probabilities for SNP markers exceeded 99.99%, indicating that the 142 SNPs panel might be adequate for parentage testing. In contrast, when utilizing STRs markers, the combined average exclusion probabilities for one and both parents were determined to be 99.8% and 99.9%, respectively. Our comprehensive study underscores the potential of SNPs in equine parentage verification, especially when compared to STRs in terms of exclusion probabilities. As a corollary, the application of SNPs for parentage verification and identification can significantly contribute to the conservation initiative for the five Moroccan horse breeds. Nonetheless, further research is required to address and replace the deficient SNPs within the panel.</p>","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":"55 3","pages":"404-409"},"PeriodicalIF":2.4,"publicationDate":"2024-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139929652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenotypic correlations of carpal gland diverticular number with production traits and its genome-wide association analysis in multiple pig populations","authors":"Dengshuai Cui,&nbsp;Longyun Li,&nbsp;Naibiao Yu,&nbsp;Sanya Xiong,&nbsp;Shijun Xiao,&nbsp;Hao Zheng,&nbsp;Zhiyong Huang,&nbsp;Yuanmei Guo,&nbsp;Lusheng Huang","doi":"10.1111/age.13407","DOIUrl":"10.1111/age.13407","url":null,"abstract":"<p>Pig carpal glands play crucial roles in territorial recognition, reproductive behavior, and information exchange; however, their effects on production traits and underlying genetic mechanisms remain unclear. In this study, 1028 pigs from six populations were counted for the carpal gland diverticular numbers (CGDNs) on the left (CGDN_L) and right (CGDN_R) legs, and their carcass and meat quality traits were assessed. The CGDNs were significantly different among the populations, and Licha Black pigs had a lower CGDN than the Bama Xiang breed. It was also significantly different between sexes, with males having more diverticula than females (<i>p</i> ≤ 0.0391). Moreover, the number was asymmetric, with CGDN_R being significantly higher than CGDN_L. Notably, CGDNs was significantly correlated with each other in phenotype and genetics and with 24-h pH, 24-h meat color score, 24-h marbling score, fat content, moisture content, sodium salt content, and saturated fatty acid content in phenotype. Furthermore, genome-wide association analyses identified seven SNPs in association with CGDNs at a 5% genome-wide significance level, all of which were located in a 1.78-Mb (35.347–37.129 Mb) region on chromosome 1. CNC10010837 and CNC10010840 were the top SNPs: both had an additive effect of 0.789 ± 0.120 on CGDN_R with <i>p</i> = 8.31E-10. These findings provide important insights into the functions and underlying genetic mechanisms of swine carpal glands.</p>","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":"55 3","pages":"396-403"},"PeriodicalIF":2.4,"publicationDate":"2024-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139911905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of two methods of sperm- and testis-mediated gene transfer in production of transgenic animals: A systematic review","authors":"Zeinab Dehghan,&nbsp;Gholamhossein Darya,&nbsp;Shayesteh Mehdinejadiani,&nbsp;Amin Derakhshanfar","doi":"10.1111/age.13404","DOIUrl":"10.1111/age.13404","url":null,"abstract":"<p>Transgenic (Tg) animal technology is one of the growing areas in biology. Various Tg technologies, each with its own advantages and disadvantages, are available for generating Tg animals. These include zygote microinjection, electroporation, viral infection, embryonic stem cell or spermatogonial stem cell-mediated production of Tg animals, sperm-mediated gene transfer (SMGT), and testis-mediated gene transfer (TMGT). However, there are currently no comprehensive studies comparing SMGT and TMGT methods, selecting appropriate gene delivery carriers (such as nanoparticles and liposomes), and determining the optimal route for gene delivery (SMGT and TMGT) for producing Tg animal. Here we aim to provide a comprehensive assessment comparing SMGT and TMGT methods, and to introduce the best carriers and gene transfer methods to sperm and testis to generate Tg animals in different species. From 2010 to 2022, 47 studies on SMGT and 25 studies on TMGT have been conducted. Mice and rats were the most commonly used species in SMGT and TMGT. Regarding the SMGT approach, nanoparticles, streptolysin-O, and virus packaging were found to be the best gene transfer methods for generating Tg mice. In the TMGT method, the best gene transfer methods for generating Tg mice and rats were virus packaging, dimethyl sulfoxide, electroporation, and liposome. Our study has shown that the efficiency of producing Tg animals varies depending on the species, gene carrier, and method of gene transfer.</p>","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":"55 3","pages":"328-343"},"PeriodicalIF":2.4,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/age.13404","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139740202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Towards identification of new genetic determinants for post-weaning diarrhea in piglets","authors":"Emil Ibragimov,&nbsp;Esben Østergaard Eriksen,&nbsp;Jens Peter Nielsen,&nbsp;Claus B. Jørgensen,&nbsp;Merete Fredholm,&nbsp;Peter Karlskov-Mortensen","doi":"10.1111/age.13406","DOIUrl":"10.1111/age.13406","url":null,"abstract":"<p>Post-weaning diarrhea in pigs is a considerable challenge in the pig farming industry due to its effect on animal welfare and production costs, as well as the large volume of antibiotics, which are used to treat diarrhea in pigs after weaning. Previous studies have revealed loci on SSC6 and SSC13 associated with susceptibility to specific diarrhea causing pathogens. This study aimed to identify new genetic loci for resistance to diarrhea based on phenotypic data. In depth clinical characterization of diarrhea was performed in 257 pigs belonging to two herds during the first 14 days post weaning. The daily diarrhea assessments were used for the classification of pigs into case and control groups. Pigs were assigned to case and control groups based only on the incidence of diarrhea in the second week of the study in order to differentiate between differences in etiology. Genome-wide association studies and metabolomics association analysis were performed in order to identify new biological determinants for diarrhea susceptibility. With the present work, we revealed a new locus for diarrhea resistance on SSC16. Furthermore, studies of metabolomics in the same pigs revealed one metabolite associated with diarrhea.</p>","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":"55 3","pages":"387-395"},"PeriodicalIF":2.4,"publicationDate":"2024-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/age.13406","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139721295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of genetic variants in protein-coding genes of Aoluguya reindeer based on the whole-genome data","authors":"Wenfeng Yi,&nbsp;Mingyue Hu,&nbsp;Lulu Shi,&nbsp;Ting Li,&nbsp;Hao Sun,&nbsp;Lihong Qin,&nbsp;Shouqing Yan","doi":"10.1111/age.13402","DOIUrl":"10.1111/age.13402","url":null,"abstract":"","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":"55 2","pages":"296-298"},"PeriodicalIF":2.4,"publicationDate":"2024-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139690981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Smartphone-based digital phenotyping for genome-wide association study of intramuscular fat traits in longissimus dorsi muscle of pigs","authors":"Yang Shen,&nbsp;Yuxi Chen,&nbsp;Shufeng Zhang,&nbsp;Ze Wu,&nbsp;Xiaoyu Lu,&nbsp;Weizhen Liu,&nbsp;Bang Liu,&nbsp;Xiang Zhou","doi":"10.1111/age.13401","DOIUrl":"10.1111/age.13401","url":null,"abstract":"<p>Intramuscular fat (IMF) content and distribution significantly contribute to the eating quality of pork. However, the current methods used for measuring these traits are complex, time-consuming and costly. To simplify the measurement process, this study developed a smartphone application (App) called Pork IMF. This App serves as a rapid and portable phenotyping tool for acquiring pork images and extracting the image-based IMF traits through embedded deep-learning algorithms. Utilizing this App, we collected the IMF traits of the longissimus dorsi muscle in a crossbred population of Large White × Tongcheng pigs. Genome-wide association studies detected 13 and 16 SNPs that were significantly associated with IMF content and distribution, respectively, highlighting <i>NR2F2</i>, <i>MCTP2</i>, <i>MTLN</i>, <i>ST3GAL5</i>, <i>NDUFAB1</i> and <i>PID1</i> as candidate genes. Our research introduces a user-friendly digital phenotyping technology for quantifying IMF traits and suggests candidate genes and SNPs for genetic improvement of IMF traits in pigs.</p>","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":"55 2","pages":"230-237"},"PeriodicalIF":2.4,"publicationDate":"2024-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139641460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}