Plant Biology最新文献

Evidence for partial mycoheterotrophy during fruiting in two leafless orchids, Cremastra aphylla and C. saprophytica. 两种无叶兰科植物——葡萄蕊和腐生兰在结实过程中部分分枝异养的证据。

IF 3.4 3区生物学

Plant Biology Pub Date : 2026-09-04 DOI: 10.1111/plb.70290

K Suetsugu

{"title":"Evidence for partial mycoheterotrophy during fruiting in two leafless orchids, Cremastra aphylla and C. saprophytica.","authors":"K Suetsugu","doi":"10.1111/plb.70290","DOIUrl":"https://doi.org/10.1111/plb.70290","url":null,"abstract":"The evolutionary transition from autotrophy to full mycoheterotrophy is often viewed as a progressive loss of photosynthetic capacity, yet comparative studies of closely related leafless orchids that retain residual photosynthesis remain scarce. The Cremastra appendiculata species complex provides a valuable system for testing whether dependence on fungal carbon varies among leafless species. We compared the nutritional modes of two closely related leafless orchids, Cremastra aphylla and C. saprophytica, at flowering and fruiting using natural-abundance stable isotope analyses and measurements of chlorophyll concentration and fluorescence. Both species retained functional, albeit limited, photosynthetic capacity. From flowering to fruiting, chlorophyll concentrations and the maximum quantum yield of photosystem II increased, whereas δ13C values decreased, a pattern consistent with enhanced photosynthetic capacity during reproductive development. However, C. aphylla consistently showed greater 13C enrichment relative to autotrophic reference plants and had a significantly lower chlorophyll concentration during flowering than C. saprophytica, indicating greater dependence on fungal carbon. These results demonstrate that leaflessness does not necessarily imply full mycoheterotrophy and, more importantly, that dependence on fungal carbon can differ even between closely related leafless species. Together with developmental-stage-dependent changes within each species, these interspecific differences suggest that the transition from partial to full mycoheterotrophy can proceed through multiple incremental steps even after leaflessness has evolved.","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148890542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stress-specific metabolic and physiological responses to drought and waterlogging in Allium cepa L. and Allium × cornutum Clementi ex Vis. 葱和秋葵对干旱和涝渍的代谢和生理响应。

IF 3.4 3区生物学

Plant Biology Pub Date : 2026-09-03 DOI: 10.1111/plb.70285

Ž Fredotović, B Soldo, B Urlić, K Radovani, E Vuko, J Puizina

{"title":"Stress-specific metabolic and physiological responses to drought and waterlogging in Allium cepa L. and Allium × cornutum Clementi ex Vis.","authors":"Ž Fredotović, B Soldo, B Urlić, K Radovani, E Vuko, J Puizina","doi":"10.1111/plb.70285","DOIUrl":"https://doi.org/10.1111/plb.70285","url":null,"abstract":"Increasing variability in water availability, caused by changing climatic conditions, is a major constraint for onion production. The present study investigated the physiological, metabolic and molecular responses of the diploid onion Allium cepa L. and the triploid hybrid Allium × cornutum Clementi ex Visiani under drought and waterlogging. Plants were exposed to controlled stress conditions, and responses were evaluated through relative water content (RWC), electrolyte leakage (EL), photosynthetic pigments, amino acid composition, total phenolic content (TPC), individual phenolic compounds and expression of antioxidant genes (CAT, SOD, AOX). Allium × cornutum showed a more pronounced response to drought, characterized by higher RWC, stable chlorophyll and carotenoid levels, increased accumulation of free amino acids and phenolic compounds, especially chlorogenic acid and quercetin glycosides, with significant upregulation of the antioxidant genes SOD and CAT. Allium cepa responded more strongly to waterlogging, showing increased amino acid levels, accumulation of quercetin and its glycosides, a marked increase in α-aminobutyric acid (AABA) concentration and higher expression of CAT and SOD. These results show distinct metabolic responses and physiological changes to drought and waterlogging, reflecting stress-specific adjustments under extreme water conditions and contributing to a better understanding of water stress adaptation in Allium species.","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148885608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of nitrogen addition on the photosynthetic responses of two dominant subtropical forest tree species to drought. 氮添加对两种亚热带优势树种对干旱光合反应的影响

IF 3.4 3区生物学

Plant Biology Pub Date : 2026-09-03 DOI: 10.1111/plb.70286

D Wang, X Liang, X Tan, D Tang, H Liu, K Hussain, Q Li, X Liu, T Zhang, Y Jin, D Yin, Y Nie, W Shen, Q Ye

{"title":"Effects of nitrogen addition on the photosynthetic responses of two dominant subtropical forest tree species to drought.","authors":"D Wang, X Liang, X Tan, D Tang, H Liu, K Hussain, Q Li, X Liu, T Zhang, Y Jin, D Yin, Y Nie, W Shen, Q Ye","doi":"10.1111/plb.70286","DOIUrl":"https://doi.org/10.1111/plb.70286","url":null,"abstract":"Drought and nitrogen deposition are global change factors that influence forest dynamics by altering tree physiology and growth. Nevertheless, their interactive effects on tree physiology (especially leaf photosynthetic traits) remain poorly understood. Here, we examined the effects of drought (D), nitrogen addition (N) and their combination (DN) on the leaf photosynthetic traits of two dominant evergreen broadleaved tree species (Schima superba and Michelia macclurei) in a subtropical forest in southern China following a 2-year treatment period. For S. superba, compared to the control (CK), D significantly decreased the leaf photosynthetic rate (Asat), stomatal conductance (gs), stomatal size (SS) and leaf nitrogen content (LNC), but increased soluble sugar content (LSS). Although N exerted no significant effects on any leaf functional traits relative to CK, DN significantly alleviated drought-induced declines in Asat, gs, SS and LNC of S. superba when compared with D. This indicates a significant interactive effect between drought and nitrogen supply: nitrogen addition only mitigated drought stress damage under water limitation, rather than improving leaf photosynthetic performance under well-watered conditions. In contrast, M. macclurei showed no significant differences in leaf photosynthesis-related traits across the D, N, DN and CK treatments. The divergent responses of the two species to D might be related to inherent differences in their hydraulic traits (turgor loss point-TLP and minimum conductance-gmin) and root characteristics. The deep-rooted S. superba displayed significantly higher TLP and gmin than the shallow-rooted M. macclurei. Overall, our results suggest that S. superba is more sensitive to D than M. macclurei. The negative effects of D on leaf photosynthesis of S. superba were significantly alleviated by N in this subtropical forest.","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148885674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Almost 200 years of anthocyanin research: What we know, what we assume and what remains unknown. 花青素研究近200年：我们知道什么，我们假设什么，还有什么未知。

IF 3.4 3区生物学

Plant Biology Pub Date : 2026-09-03 DOI: 10.1111/plb.70283

N Choudhary, N Khatun, B Pucker

引用次数: 0

The impact of seasonal variations in inner bark width and xylem growth on irreversible annual stem diameter measurements. 内层树皮宽度和木质部生长的季节变化对不可逆的年度茎粗测量的影响。

IF 3.4 3区生物学

Plant Biology Pub Date : 2026-08-25 DOI: 10.1111/plb.70281

J Gričar, K Eler

{"title":"The impact of seasonal variations in inner bark width and xylem growth on irreversible annual stem diameter measurements.","authors":"J Gričar, K Eler","doi":"10.1111/plb.70281","DOIUrl":"https://doi.org/10.1111/plb.70281","url":null,"abstract":"To better interpret continuous xylem growth measurements from dendrometers, we evaluated how the widths of developing xylem and phloem, along with seasonal trends in inner bark width (conducting phloem, non-conducting phloem and the youngest periderm), contribute to irreversible annual stem diameter changes in Pinus nigra, Fraxinus ornus and Ostrya carpinifolia. Histometric analyses were performed on microcores taken from tree stems in 2020. The seasonal patterns of xylem and phloem formation, as well as changes in inner bark width and xylem growth, varied both within and among species. Of the total annual stem radial increment, xylem and phloem increments accounted for 40.5% and 8.1% in P. nigra, 9.6% and 2.5% in F. ornus, and 17.6% and 7.1% in O. carpinifolia, respectively. The remaining portion comprised inner bark tissues, with non-conducting phloem forming the largest fraction and thus exerting a major influence on variation in inner bark width. Despite seasonal variation in inner bark width, its width at the beginning and end of the growing season was similar across all species, indicating that subtracting inner bark width from the annual stem radial increment at the end of the growing season provides a reasonable estimate of annual xylem increment. However, seasonal changes in inner bark width differed from those of xylem growth, suggesting that xylem phenology cannot be reliably assessed without considering its ontogeny at the cellular level.","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148816827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant endemism of the Balkan high-mountain fens: a community-level synthesis and their climate change perspective. 巴尔干高山沼泽的植物特有种：群落水平的综合及其气候变化视角。

IF 3.4 3区生物学

Plant Biology Pub Date : 2026-08-16 DOI: 10.1111/plb.70279

M Hájek, P Hájková, M Chobanova, Z Plesková, E Bergmeier, P Lazarević, D Szokala, N Berisha, L Shuka, D Dítě, T Peterka, R Čusterevska, D Sopotlieva, J Divíšek, I Goia, B Vrábel, Đ Milanović, S Hristovski, D Stešević, P Bureš, I Apostolova

{"title":"Plant endemism of the Balkan high-mountain fens: a community-level synthesis and their climate change perspective.","authors":"M Hájek, P Hájková, M Chobanova, Z Plesková, E Bergmeier, P Lazarević, D Szokala, N Berisha, L Shuka, D Dítě, T Peterka, R Čusterevska, D Sopotlieva, J Divíšek, I Goia, B Vrábel, Đ Milanović, S Hristovski, D Stešević, P Bureš, I Apostolova","doi":"10.1111/plb.70279","DOIUrl":"https://doi.org/10.1111/plb.70279","url":null,"abstract":"The phenomenon of Balkan high-mountain fen plant endemism, unique within Europe, remains poorly studied. We compiled an original dataset of wetland vegetation-plot data across nine countries where Balkan endemics and subendemics occur. We tested the diversity patterns for endemics, non-endemic habitat specialists of bryophytes and vascular plants and matrix-derived taxa with respect to climate, palaeoclimate, edaphic and topographic factors. We used Spearman correlations, Spatial Autoregressive Error Models, Canonical Correspondence Analysis and Principal Component Analysis of abiotic factors with richness patterns projected using Generalised Additive Models. Two major hotspots of fen endemism, centred around the Rila and Korab Mts, were identified. Species composition of endemics generally follows an east-to-west gradient that coincides with palaeoclimatic patterns. Palaeoclimate was influential for community-scale richness of bryophytes and narrow-range endemics, the latter being positively affected by the Bølling-Allerød precipitation peak and the wet-tundra climate during the glacial. The richness of other groups was affected by recent climate, edaphic factors and topography. Water pH differentiated major vegetation types and affected the richness of matrix-derived taxa and fen bryophytes, but had a minor impact on Balkan endemics, except those associated with serpentinite. Several endemic taxa usually co-exist even in small wetland patches. Palaeoclimate shows that fen endemics evidently experienced drier, warmer summers than today over the last 21,000 years; yet they were more affected by past precipitation. If we conserve Balkan high-mountain fens, their catchment properties and hydrology, and maintain moderate grazing, we increase the likelihood that their endemics will withstand ongoing climate change.","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148758770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strawberry micropropagation simplifies endomicrobiome and highlights potential for in vitro biotization with beneficial rhizobacteria. 草莓的微繁殖简化了体内微生物组，并突出了与有益的根瘤菌体外生物化的潜力。

IF 3.4 3区生物学

Plant Biology Pub Date : 2026-08-16 DOI: 10.1111/plb.70278

A M G Sepúlveda, E da Conceição Jesus, Y C Molina, V H Buttrós, L Q Carvalho, M Pasqual, J Dória

{"title":"Strawberry micropropagation simplifies endomicrobiome and highlights potential for in vitro biotization with beneficial rhizobacteria.","authors":"A M G Sepúlveda, E da Conceição Jesus, Y C Molina, V H Buttrós, L Q Carvalho, M Pasqual, J Dória","doi":"10.1111/plb.70278","DOIUrl":"https://doi.org/10.1111/plb.70278","url":null,"abstract":"Strawberry micropropagation produces uniform, disease-free planting material. However, repeated in vitro subculturing can simplify the endophytic microbiome, reducing plant vigour and acclimatization success. This study investigated the impact of successive in vitro generations on the endomicrobiome of Fragaria × ananassa cv. San Andreas and determined if targeted biotization with beneficial rhizobacteria can enhance plant performance. Profiled endophytic bacterial and fungal communities were analysed in the mother plant and across three in vitro generations using amplicon sequencing of the 16S rRNA gene and ITS markers. Ten plant growth-promoting bacterial strains were tested in vitro. The most effective strains were combined into consortia, and their impact on growth, survival and phenolic and flavonoid accumulation was assessed under in vitro and ex vitro acclimatization conditions. Successive subcultures simplified the endophytic assemblage. The mother plant exhibited substantially higher bacterial alpha-diversity than in vitro generations, with richness and Shannon diversity declining significantly (P <0.001) across all subcultures. In vitro biotization with A. brasilense Ab-V5 and A. brasilense Ab-V6 increased total dry biomass from 190.63 mg in the control to 216.75 mg and 209.11 mg, respectively. Under ex vitro conditions, several inoculated treatments achieved 100% survival compared with 75% in the non-inoculated control. Prolonged in vitro maintenance reduces the complexity of the strawberry endomicrobiome by imposing a selective bottleneck. Targeted introduction of beneficial bacteria partially compensates by enhancing growth, survival and secondary metabolite accumulation, supporting microbiome-assisted strategies to improve tissue culture performance and ex vitro establishment.","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148758619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Drought-induced embolism shapes sex-specific responses by regulating hydraulic conductivity in Populus cathayana and Salix babylonica. 干旱诱导的栓塞通过调节白杨和巴比伦柳的水力传导来形成性别特异性反应。

IF 3.4 3区生物学

Plant Biology Pub Date : 2026-08-16 DOI: 10.1111/plb.70280

L Tan, X Zhang, F Chen, L Xia, S Zhang

{"title":"Drought-induced embolism shapes sex-specific responses by regulating hydraulic conductivity in Populus cathayana and Salix babylonica.","authors":"L Tan, X Zhang, F Chen, L Xia, S Zhang","doi":"10.1111/plb.70280","DOIUrl":"https://doi.org/10.1111/plb.70280","url":null,"abstract":"Drought is a key constraint on plant growth and development. Poplars and willows are dioecious plants, and sex-specific responses to drought differ across species. However, little is known about how xylem embolism drives sex-specific hydraulic failure. In this study, male and female Populus cathayana and Salix babylonica were used to investigate the role of embolism in sex-specific drought responses. Drought significantly reduced plant growth and photosynthetic capacity, increased membrane damage and reactive oxygen species accumulation, altered vessel structure and aggravated embolism. P. cathayana males maintained higher net photosynthetic rates than females, whereas S. babylonica females performed better than males under drought stress. P. cathayana males exhibited larger vessel area and diameter but lower density than females, while the opposite sex pattern was observed in S. babylonica. In terms of hydraulic function, male P. cathayana and female S. babylonica had lower embolism degrees, higher hydraulic conductivity and maintained more stable conductivity than their opposite sexes. Embolism degree was strongly correlated with both the standardized vessel structure index and the standardized photosynthetic index (R2 = 0.81 and 0.93 for P. cathayana; 0.78 and 0.91 for S. babylonica, respectively). Overall, male P. cathayana and female S. babylonica were more resistant to embolism, sustaining higher photosynthetic performance and growth. These findings suggest that drought alters xylem vessel structure, thereby inducing embolism and restricting growth, which forms the core mechanism underlying sex-specific drought responses in P. cathayana and S. babylonica.","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148766142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Floral adaptation and modular trait evolution associated with a shift from bee to hummingbird pollination. 从蜜蜂传粉到蜂鸟传粉的转变与花的适应和模块化特征进化有关。

IF 3.4 3区生物学

Plant Biology Pub Date : 2026-08-06 DOI: 10.1111/plb.70277

P Juárez, K M Kay

{"title":"Floral adaptation and modular trait evolution associated with a shift from bee to hummingbird pollination.","authors":"P Juárez, K M Kay","doi":"10.1111/plb.70277","DOIUrl":"https://doi.org/10.1111/plb.70277","url":null,"abstract":"Pollination shifts result in floral divergence, yet how such shifts influence variation and correlation among floral traits is unclear. We examine whether a transition from bee to hummingbird pollination in sister species of Neotropical spiral gingers - Costus kuntzei, with ancestral bee pollination, and Costus wilsonii, with derived hummingbird pollination - drives phenotypic variation, functional integration and morphological modularity. Using field measurements and observations, we assess pollinator assemblages and pollination syndrome divergence by quantifying a suite of floral traits related to shape, size, colour, scent and nectar reward. We then test whether the shift from bee to hummingbird pollination has led to differences in floral phenotypic variation and integration, and we use geometric morphometric analyses to evaluate modularity hypotheses based on floral function and genetic or developmental constraints. We find that C. wilsonii exhibits changes in, and loss of, multiple floral traits, along with extreme specialization on a single hermit hummingbird species. In contrast, C. kuntzei is visited by a more species-rich pollinator assemblage of euglossine bees and a tabanid fly. Both plant species show similar patterns of floral variation, integration and modularity, with reduced floral variation and modularity patterns concentrated in traits involved in pollinator fit and pollen transfer. The findings suggest that phenotypic patterns reflect functional traits shaped by divergent pollinator-mediated adaptation. This floral adaptation has resulted in stable patterns of trait variation and correlation, consistent with selection for efficient pollination and pollinator fit in both species.","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148682803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Saturating mutagenesis on the Ser118 residue responsible for the spectral tuning mechanism of the orange/green-reversible cyanobacteriochrome AM1_1499g1. 橙/绿可逆蓝藻色素AM1_1499g1光谱调谐机制Ser118残基的饱和诱变

IF 3.4 3区生物学

Plant Biology Pub Date : 2026-08-06 DOI: 10.1111/plb.70275

A Yoneda, T Suzuki, R Narikawa

{"title":"Saturating mutagenesis on the Ser118 residue responsible for the spectral tuning mechanism of the orange/green-reversible cyanobacteriochrome AM1_1499g1.","authors":"A Yoneda, T Suzuki, R Narikawa","doi":"10.1111/plb.70275","DOIUrl":"https://doi.org/10.1111/plb.70275","url":null,"abstract":"Cyanobacteriochromes (CBCRs) are widely distributed photoreceptors in cyanobacteria, crucial for regulating light-responsive phenomena. CBCR GAF domains form a stable covalent bond with a linear tetrapyrrole chromophore via the canonical Cys residue and exhibit reversible photoconversion. The DXCF (Asp-Xxx-Cys-Phe) -type CBCRs, a dominant subgroup across various clades, are characterized by a highly conserved second Cys residue involved in both phycocyanobilin (PCB)-to-phycoviolobilin (PVB) isomerization and reversible ligation to the chromophore. The AM1_1499g1 GAF domain, categorized as a DXCF-type CBCR, is unique due to the evolutionary loss of this second Cys residue, which is substituted by Ser118. This domain exhibits an orange/green-reversible photocycle alongside thermochromism in its photoproduct state, suggesting a novel spectral tuning mechanism to be elucidated. To elucidate the molecular basis of the spectral tuning mechanism in AM1_1499g1, we performed saturation mutagenesis at the Ser118 position and comprehensively performed spectral analyses of the resulting S118X variants. Spectral analyses classified the S118X mutants into five distinct functional groups based on PCB-to-PVB isomerization activity, thermochromism and photocycle property. These results lead us to propose that the side chain property at Ser118, located near the chromophore centre, affects the chromophore conformation, especially the A-ring conformation, thereby controlling the domain's spectral properties. Framed within the evolutionary transition of AM1_1499g1, our findings demonstrate that single-site mutagenesis at Ser118 is sufficient to recapitulate a broad range of spectral properties. This variation functionally mimics the natural evolutionary divergence of XRG (extended red/green)-type CBCR domains, highlighting the exceptional molecular plasticity of this photoreceptor family.","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148682798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0