Plant Biology最新文献

{"title":"Computational analysis and expression profiling of NAC transcription factor family involved in biotic stress response in Manihot esculenta","authors":"A. H. Abdoulaye, C. Yuhua, Z. Xiaoyan, Y. Yiwei, H. Wang, C. Yinhua","doi":"10.1111/plb.13715","DOIUrl":"https://doi.org/10.1111/plb.13715","url":null,"abstract":"<jats:list list-type=\"bullet\"> <jats:list-item>The Nascent polypeptide‐Associated Complex (NAC) family is among the largest plant‐specific TF families and plays an important role in plant growth, development, and stress responses.</jats:list-item> <jats:list-item>NAC TFs have been extensively studied in plants such as rice and <jats:italic>Arabidopsis</jats:italic>; however, their characterization, functions, evolution, and expression patterns in <jats:italic>Manihot esculenta</jats:italic> (cassava) under environmental stress remain largely unexplored. Here, we used bioinformatic analyses and biotic stress responses to investigate the physicochemical properties, chromosome location, phylogeny, gene structure, expression patterns, and <jats:italic>cis</jats:italic>‐elements in promoter regions of the NAC TFs in cassava.</jats:list-item> <jats:list-item>We identified 119 <jats:italic>M. esculenta</jats:italic> NAC (MeNAC) gene families, unevenly distributed on 16 chromosomes. We investigated expression patterns of all identified MeNAC TFs under <jats:italic>Xanthomonas axonopodis</jats:italic> pv. <jats:italic>manihotis</jats:italic> (<jats:italic>Xam</jats:italic>) infection, strain CHN11, at different time points. Only 20 MeNAC TFs showed expression of significant bacterial resistance. Six MeNACs (MeNAC7, 26, 63, 65, 77, and 113) were selected for functional analysis. qRT‐PCR assays revealed that MeNAC7, 26, 63, 65, 77, and 113 were induced in response to <jats:italic>Xam</jats:italic>CHN11 infection and may participate in the molecular interaction of cassava and bacterial blight. Interestingly, MeNAC26, MeNAC63, MeNAC65, and MeNAC113 responded to <jats:italic>Xam</jats:italic>CHN11 infection at 3 h post‐inoculation. Furthermore, we identified 13 stress‐related <jats:italic>cis</jats:italic>‐elements in promoter regions of the MeNAC genes that are involved in diverse environmental stress responses. Phylogenetic analysis revealed that MeNAC genes with similar structures and motif distributions were grouped.</jats:list-item> <jats:list-item>This study provides valuable insights into the evolution, diversity, and characterization of MeNAC TFs. It lays the groundwork for a better understanding of their biological roles and molecular mechanisms in cassava.</jats:list-item> </jats:list>","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142185797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Be prepared: how does discontinuous hydration in Tabebuia heterophylla seeds induce stress tolerance in seedlings?","authors":"G. da Silva Dias, J. T. L. Chaves, T. R. S. Santos, Q. S. Garcia, M. A. S. Artur, E. M. Bicalho","doi":"10.1111/plb.13719","DOIUrl":"https://doi.org/10.1111/plb.13719","url":null,"abstract":"<jats:list list-type=\"bullet\"> <jats:list-item>Discontinuous hydration and dehydration (HD) cycles refer to controlled imbibition followed by dehydration before seed germination. Here, we investigated whether the level of imbibition before HD cycles affects the physiology of <jats:italic>Tabebuia heterophylla</jats:italic> seeds and seedlings.</jats:list-item> <jats:list-item>Seeds were imbibed for 10 h (T1; phase I of imbibition) or 35 h (T2; phase II), dehydrated, and progressively rehydrated one to four times (HD cycles). Germination and biochemical parameters (membrane integrity; total soluble, reducing, and nonreducing (NRS) sugars; proteins, amino acids, proline, H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub>, catalase, ascorbate peroxidase, and glutathione reductase activity) were quantified at the last rehydration step of each cycle. Biometric and biochemical parameters (including pigments) were analysed in seedlings 60 days after germination.</jats:list-item> <jats:list-item>HD cycles at T1 led to reduced seed germination and greater plasma membrane damage, higher enzyme activity (catalase and glutathione reductase) and accumulation of NRS, total amino acids, and proline compared to the controls and T2 treatment. Cellular damage became more severe with more HD cycles. HD cycles at T2 synchronized germination regardless of the number of cycles and also had a priming effect. T2 seeds had less NRS, total amino acids, and proline content than T1. HD cycles at T1 produced seedlings with higher carotenoid and total chlorophyll content than controls and T2, while seedlings from HD cycles at T2 had higher amounts of osmoprotectants.</jats:list-item> <jats:list-item>HD cycles at T2 benefited seeds and seedlings more than at T1. This suggests that the physiological and biochemical effects of HD cycles in seeds modulate seedling plasticity, depending on water availability, potentially promoting increased tolerance to recurrent droughts that will be intensified with ongoing climate changes.</jats:list-item> </jats:list>","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142185796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Further evidence for endozoochory in a mycoheterotrophic orchid Cyrtosia septentrionalis: seed dispersal by the masked palm civet Paguma larvata","authors":"K. Suetsugu","doi":"10.1111/plb.13718","DOIUrl":"https://doi.org/10.1111/plb.13718","url":null,"abstract":"<jats:list list-type=\"bullet\"> <jats:list-item><jats:italic>Cyrtosia septentrionalis</jats:italic>, an orchid species, is notable for its sausage‐shaped red fleshy fruits, which can reach up to 10 cm in length. Previous research identified frugivorous and omnivorous birds, especially the brown‐eared bulbul <jats:italic>Hypsipetes amaurotis</jats:italic> (Pycnonotidae, Passeriformes), as key seed dispersers of <jats:italic>C. septentrionalis</jats:italic> in natural habitats. This finding challenges conventional belief that orchid seeds are primarily wind‐dispersed. Moreover, given the rarity of specialized co‐evolutionary relationships between fruits and frugivores, <jats:italic>C. septentrionalis</jats:italic> may also rely on mammals for seed dispersal in certain regions.</jats:list-item> <jats:list-item>We aimed to explore the fruit consumption habits of animals other than birds. Motion sensor‐equipped cameras were used to observe interactions with the fruits of <jats:italic>C. septentrionalis</jats:italic> and assess the viability of seeds post‐consumption.</jats:list-item> <jats:list-item>The observations revealed three animal species consuming the fruits, with the masked palm civet <jats:italic>Paguma larvata</jats:italic> (Viverridae, Carnivora) identified as a dominant consumer in the investigated population. Microscopy analysis of seeds retrieved from <jats:italic>P. larvata</jats:italic> faeces indicated that the seeds remained intact and viable after passing through the digestive system of this carnivorous mammal.</jats:list-item> <jats:list-item>The above discovery suggests a potential role for carnivorous mammals in seed dispersal of <jats:italic>C. septentrionalis</jats:italic>, alongside birds, thus broadening our understanding of the complex seed dispersal strategies employed by orchids.</jats:list-item> </jats:list>","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142185798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long‐term methylome changes after experimental seed demethylation and their interaction with recurrent water stress in Erodium cicutarium (Geraniaceae)","authors":"F. Balao, M. Medrano, P. Bazaga, O. Paun, C. Alonso","doi":"10.1111/plb.13713","DOIUrl":"https://doi.org/10.1111/plb.13713","url":null,"abstract":"<jats:list list-type=\"bullet\"> <jats:list-item>The frequencies and lengths of drought periods are increasing in subtropical and temperate regions worldwide. Epigenetic responses to water stress could be key for plant resilience to these largely unpredictable challenges. Experimental DNA demethylation, together with application of a stress factor is an appropriate strategy to reveal the contribution of epigenetics to plant responses to stress.</jats:list-item> <jats:list-item>We analysed leaf cytosine methylation changes in adult plants of the annual Mediterranean herb, <jats:italic>Erodium cicutarium</jats:italic>, in a greenhouse, after seed demethylation with 5‐Azacytidine and/or recurrent water stress. We used bisulfite RADseq (BsRADseq) and a newly reported reference genome for <jats:italic>E. cicutarium</jats:italic> to characterize methylation changes in a 2 × 2 factorial design, controlling for plant relatedness.</jats:list-item> <jats:list-item>In the long term, 5‐Azacytidine treatment alone caused both hypo‐ and hyper‐methylation at individual cytosines, with substantial hypomethylation in CG contexts. In control conditions, drought resulted in a decrease in methylation in all but CHH contexts. In contrast, the genome of plants that experienced recurrent water stress and had been treated with 5‐Azacytidine increased DNA methylation level by ca. 5%.</jats:list-item> <jats:list-item>Seed demethylation and recurrent drought produced a highly significant interaction in terms of global and context‐specific cytosine methylation. Most methylation changes occurred around genic regions and within Transposable Elements. The annotation of these Differentially Methylated Regions associated with genes included several with a potential role in stress responses (e.g., PAL, CDKC, and ABCF), confirming an epigenetic contribution in response to stress at the molecular level.</jats:list-item> </jats:list>","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142185806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Species‐specific functional trait responses in two species coexisting along a shore‐to‐inland dune gradient","authors":"R. Bermúdez, J. Sánchez Vilas, R. Retuerto","doi":"10.1111/plb.13710","DOIUrl":"https://doi.org/10.1111/plb.13710","url":null,"abstract":"<jats:list list-type=\"bullet\"> <jats:list-item>Coastal dunes are characterised by strong gradients of abiotic stress, typically increasing in severity from inland areas towards the shoreline. Thus, dune gradients represent unique opportunities to study intraspecific responses to environmental changes and to investigate which factors drive community change. This study aims to examine functional trait variation in two coexisting species in response to environmental changes along a dune gradient in NW Spain. Trait convergence was also investigated and compared between both ends of the gradient.</jats:list-item> <jats:list-item>We measured functional leaf traits related to plant efficiency in the use of light, water and nutrients, also possible stressors (salt content and pH) and availability of limiting resources (water and nutrients) in the soil.</jats:list-item> <jats:list-item>Most soil variables showed changes following a non‐directional gradient. Differences in soil variables were site specific and depended on growth of the study species. Structural and functional traits depended on species and/or plant position on the gradient, except for effective quantum yield of PSII and leaf δ<jats:sup>15</jats:sup>N. The pattern of variation was mostly directional for reflectance indices related to leaf physiology. Multivariate analyses showed significant interspecific differences in the set of traits they exhibited along positions in the gradient. Species also differed in the combination of traits selected under given environmental conditions.</jats:list-item> <jats:list-item>Coexisting species display a specific set of traits that reflects different strategies to environmental stress. Our study highlights the overly simplistic nature of some previous studies that assume dune gradients are monotonically directional, without considering that these gradients may be differentially modified by species activity.</jats:list-item> </jats:list>","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142185799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unravelling soybean responses to early and late Tetranychus urticae (Acari: Tetranychidae) infestation","authors":"K. Ruffatto, L. C. O. da Silva, C. d. O. Neves, S. G. Kuntzler, J. C. de Lima, F. A. Almeida, V. Silveira, F. M. Corrêa, L. V. P. Minello, L. Johann, R. A. Sperotto","doi":"10.1111/plb.13717","DOIUrl":"https://doi.org/10.1111/plb.13717","url":null,"abstract":"<jats:list list-type=\"bullet\"> <jats:list-item>Soybean is a crucial source of food, protein, and oil worldwide that is facing challenges from biotic stresses. Infestation of <jats:italic>Tetranychus urticae</jats:italic> Koch (Acari: Tetranychidae) stands out as detrimentally affecting plant growth and grain production. Understanding soybean responses to <jats:italic>T. urticae</jats:italic> infestation is pivotal for unravelling the dynamics of mite–plant interactions. We evaluated the physiological and molecular responses of soybean plants to mite infestation after 5 and 21 days.</jats:list-item> <jats:list-item>We employed visual/microscopy observations of leaf damage, H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> accumulation, and lipid peroxidation. Additionally, the impact of mite infestation on shoot length/dry weight, chlorophyll concentration, and development stages was analysed. Proteomic analysis identified differentially abundant proteins (DAPs) after early (5 days) and late (21 days) infestation. Furthermore, GO, KEGG, and protein–protein interaction analyses were performed to understand effects on metabolic pathways.</jats:list-item> <jats:list-item>Throughout the analysed period, symptoms of leaf damage, H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> accumulation, and lipid peroxidation consistently increased. Mite infestation reduced shoot length/dry weight, chlorophyll concentration, and development stage duration. Proteomics revealed 185 and 266 DAPs after early and late mite infestation, respectively, indicating a complex remodelling of metabolic pathways. Photorespiration, chlorophyll synthesis, amino acid metabolism, and Krebs cycle/energy production were impacted after both early and late infestation. Additionally, specific metabolic pathways were modified only after early or late infestation.</jats:list-item> <jats:list-item>This study underscores the detrimental effects of mite infestation on soybean physiology and metabolism. DAPs offer potential in breeding programs for enhanced resistance. Overall, this research highlights the complex nature of soybean response to mite infestation, providing insights for intervention and breeding strategies.</jats:list-item> </jats:list>","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142185807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fungus gnat pollination in Arisaema urashima: the interplay of lethal traps and mutualistic nurseries.","authors":"K Suetsugu, H Nishigaki, R Sato, S Kakishima, E Ishitani, S Fukushima, S Sugiura, M Sueyoshi","doi":"10.1111/plb.13714","DOIUrl":"https://doi.org/10.1111/plb.13714","url":null,"abstract":"<p><p>While most flowering plants engage in mutualistic interactions with their pollinators, Arisaema species employ a unique, seemingly antagonistic strategy by imprisoning and causing the pollinators to perish within their spathes. Recent studies have revealed that Arisaema thunbergii primarily relies on a fungus gnat, Leia ishitanii, with some individuals possibly escaping female spathes after oviposition. We investigated interactions between A. urashima and its pollinating fungus gnats, given that A. urashima is closely related to A. thunbergii. Specifically, we tested whether decaying A. urashima serve as brood-sites for some pollinators and whether these pollinators can escape seemingly lethal floral traps. We retrieved A. urashima spathes together with adult insect corpses trapped within the spathes and incubated the spathes to see if conspecific insects emerged. In addition, under laboratory conditions, we observed the escape behaviour of Sciophila yokoyamai, whose next-generation adults most frequently emerge from the decaying spathes. Our findings indicate that S. yokoyamai almost always escapes from the female spathe after oviposition while using the inflorescence as a nursery. In contrast, other pollinators of A. urashima, including Mycetophila spp., remain trapped and perished within the spathes. This study demonstrates that A. urashima spathes can function both as lethal traps and mutualistic nurseries, with outcomes differing among pollinator species. Our results also suggest that the contribution of certain pollinators to Arisaema reproduction is underestimated or even neglected, given that information on their pollinator assemblages has been based on floral visitors trapped within the inflorescences.</p>","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142124386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Putting the puzzle together: the relationship between floral characters and pollinator morphology determines pollination mode in the fig-fig wasp mutualism.","authors":"N Castro-Cárdenas, S Martén-Rodríguez, S Vázquez-Santana, G Cornejo-Tenorio, A Navarrete-Segueda, G Ibarra-Manríquez","doi":"10.1111/plb.13712","DOIUrl":"https://doi.org/10.1111/plb.13712","url":null,"abstract":"<p><p>The diversification of angiosperms has largely been attributed to adaptive radiation of their pollination and mating systems, which are relevant drivers of the macroevolution processes. The fig (Ficus, Moraceae) and fig wasp (Agaonidae, Hymenoptera) interaction is an example of obligate mutualism. Passive and active pollination modes have been associated with morphological traits in both partners. However, more information is required to assess the relationship between floral traits and pollination modes, particularly in Neotropical Ficus species. This study evaluates the morphological traits of figs and fig wasps regarding pollination modes in species belonging to Neotropical Ficus sections (three species each of Americanae and Pharmacosycea). Pollination mode was identified by floral morphology, anther/ovule ratio, and specialized structures fig wasps use for pollen transport (pollen pocket and coxal combs). Fig species in sect. Americanae are actively pollinated because pistillate flowers form a synstigma, present anther/ovule ratios <0.11, and their pollinator Pegoscapus fig wasps have pollen pockets and coxal combs. In contrast, species in sect. Pharmacosycea have free pistillate flowers, with anther/ovule ratios >0.27; they are pollinated by Tetrapus wasps, which lack specialized structures to carry pollen. Each species of Ficus was associated with a single morphospecies of fig wasp. The results support previous contributions that consider reciprocal morphological traits between fig species and their pollinating wasps as evidence of a close co-evolutionary history.</p>","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142118575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pollinator response to yellow UV-patterned versus white UV-patternless flower dimorphism in Anemone palmata","authors":"N. L. Rodríguez-Castañeda,&nbsp;M. L. Buide,&nbsp;M. Arista,&nbsp;E. Narbona,&nbsp;P. L. Ortiz","doi":"10.1111/plb.13702","DOIUrl":"10.1111/plb.13702","url":null,"abstract":"<p>\u0000 \u0000 </p>","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/plb.13702","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142118574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The transmembrane domain of the rice small protein OsS1Fa1 is responsible for subcellular localization and drought tolerance","authors":"J. S. Kwak,&nbsp;K. H. Lee,&nbsp;W. K. Min,&nbsp;S. J. Lee,&nbsp;J. T. Song,&nbsp;H. S. Seo","doi":"10.1111/plb.13711","DOIUrl":"10.1111/plb.13711","url":null,"abstract":"<p>\u0000 \u0000 </p>","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/plb.13711","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142046041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}