Plant Biology最新文献

Influence of drought stress on the metabolite and ion composition in nectar and nectaries of different day- and night-flowering Nicotiana species.

IF 4.2 3区生物学

Plant Biology Pub Date : 2025-02-18 DOI: 10.1111/plb.70000

T Göttlinger, D Naegel, J E Dick, G Lohaus

{"title":"Influence of drought stress on the metabolite and ion composition in nectar and nectaries of different day- and night-flowering Nicotiana species.","authors":"T Göttlinger, D Naegel, J E Dick, G Lohaus","doi":"10.1111/plb.70000","DOIUrl":"https://doi.org/10.1111/plb.70000","url":null,"abstract":"The frequencies of droughts worldwide will increase in the future due to climate changes. Nectar composition of plant species varies in relation to pollinator and can also be influenced by drought. We investigated both different pollinated species and the effects of drought in parallel. In addition, the influence of drought on nectar production and metabolism in nectaries should be investigated, since very little is currently known about this. The influence of drought stress on nectaries, nectar and leaves of 4 day- and night-flowering Nicotiana species (pollinated by sunbirds, hummingbirds, hawkmoths or bats) were investigated. The nectar volume, as well as metabolite concentrations (sugars, amino acids), inorganic ions and starch were measured. PCA and PERMANOVA were applied to determine the relative importance of different drought conditions on metabolism of nectaries and nectar. Drought stress led to changes in composition of nectaries and nectar in all four Nicotiana species. The day-flowering species had relatively similar changes, whereas the night-flowering species differed from these and also from each other. Quantities of sugars, amino acids and inorganic ions per flower decreased sharply in all Nicotiana species because of a strong decrease in nectar volume. Drought stress not only compromises plant growth but also nectar secretion and composition. These changes are likely to affect plant-pollinator interactions and may negatively impact successful pollination.","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":" ","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Iron homeostasis in the annual and perennial stem zones of Arabis alpina.

IF 4.2 3区生物学

Plant Biology Pub Date : 2025-02-18 DOI: 10.1111/plb.13756

A Sergeeva, H-J Mai, P Bauer

{"title":"Iron homeostasis in the annual and perennial stem zones of Arabis alpina.","authors":"A Sergeeva, H-J Mai, P Bauer","doi":"10.1111/plb.13756","DOIUrl":"https://doi.org/10.1111/plb.13756","url":null,"abstract":"Plants store nutrients, including the micronutrient iron, in bioavailable forms to support growth in subsequent seasons. The perennial lifestyle is preponderant in nature. Arabis alpina is a perennial model organism with a particular architecture of branches having proximal perennial (PZ) and distal annual (AZ) stem zones with distinct anatomy and physiology. PZ undergoes secondary growth and stores carbon-rich macromolecules. Here, we investigated iron homeostasis in PZ versus AZ. We localized iron in stem internodes of PZ and AZ using Perls staining and ferritin immunodetection in the wild-type Pajares (Paj) and perpetual flowering 1 mutant. We mined transcriptome data of the developing PZ and AZ. Iron accumulated at various development stages in PZ and AZ. Iron levels in PZ were ca. two-fold higher than in AZ, irrespective of flowering. Iron was predominantly located within plastid-bound ferritin. Gene expression patterns supported the significance of ferritin and demonstrated enrichment of transcripts related to iron homeostasis within stems. Distinct patterns of expression among iron homeostasis genes were observed in relation to iron content in PZ and AZ, indicating tissue-specific regulatory mechanisms governing iron accumulation. Secondary growth in PZ plays a role in iron storage in the perennial A. alpina, while AZ may transiently store iron for seed production. Future research will address different iron homeostasis signalling pathways in AZ and PZ.","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":" ","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

B-class gene GLOBOSA - a facilitator for enriched species diversity of Salvia in the New World?

IF 4.2 3区生物学

Plant Biology Pub Date : 2025-02-18 DOI: 10.1111/plb.70002

S Wetters, P Nick

{"title":"B-class gene GLOBOSA - a facilitator for enriched species diversity of Salvia in the New World?","authors":"S Wetters, P Nick","doi":"10.1111/plb.70002","DOIUrl":"https://doi.org/10.1111/plb.70002","url":null,"abstract":"The genus Salvia, comprising around 1000 species, half of which are found in the New World, belongs to the taxonomically most challenging genera within the Lamiaceae. A part of this diversity can be ascribed to the shape and expansion of the corolla and stamen structures, because changes in geometry of the sexual organs and attractance of pollinators might establish propagation barriers. However, the structural, functional, and evolutionary context of the underlying genes has not yet been elaborated. In this study, we analyse a large set of flowers from Salvia species of different geographic origin and use this morphometric framework to address gene expression and phylogenetic analysis of the MADS-box B-class gene, GLOBOSA. We examined expression of GLOBOSA in petals and anthers throughout anthesis for both Salvia pratensis L., as species from Europe, and the American Salvia elegans Vahl. Structural analysis of the B-class genes reveals typical MADS-MIKC-type composition. When we infer phylogenies for GLOBOSA and its binding partner DEFICIENS, we see a genus-wide duplication of DEFICIENS in Salvia and a specific duplication of GLOBOSA in Salvia species from the New World. Based on the first description of flowering genes in the genus Salvia, we arrive at a working model, where a duplication of GLOBOSA enabled the intense radiation of New World Salvia by neo-functionalization of a flower identity gene for morphogenetic control of corolla and anther geometry. We propose that the genus Salvia can be used as paradigm to address the role of EvoDevo for plant speciation.","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":" ","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coupled hydraulic and whole-plant economic strategies in twenty warm-temperate woody species.

IF 4.2 3区生物学

Plant Biology Pub Date : 2025-02-13 DOI: 10.1111/plb.13772

Q Li, N Wang, X Liu, X Sun, X Li, N Du, H Wang, R Wang

{"title":"Coupled hydraulic and whole-plant economic strategies in twenty warm-temperate woody species.","authors":"Q Li, N Wang, X Liu, X Sun, X Li, N Du, H Wang, R Wang","doi":"10.1111/plb.13772","DOIUrl":"https://doi.org/10.1111/plb.13772","url":null,"abstract":"The iso- to anisohydric continuum describes how plant regulate water potential and has been used to classify species hydraulic strategies. The slow to fast continuum is a whole-plant strategy for resource acquisition and utilization. The relationship between hydraulic and whole-plant economic strategy could provide a comprehensive method for assessing plants performance. We quantified the degree of isohydricity of 20 woody species in a warm temperate forest. We also measured other functional traits associated with hydraulic and economic strategies (leaf gas exchange, pressure-volume traits, predawn and midday water potential, native and maximum stem hydraulic conductivity, Huber value, and wood density), then explored the underlying trade-offs. Pearson correlations and PCA were performed to assess relationships between isohydricity and other functional traits. We found a coordinated series of iso- anisohydric and slow-fast spectra, where species percentage loss of hydraulic conductivity (PLC) and wood density (WD) were the two most powerful proxies. Along the coordinated continuum, the anisohydric species had higher leaf gas exchange, PLC, and water potential at the turgor loss point, and lower WD than the isohydrics. We found that isohydric species have high drought tolerance, giving them a greater chance of survival than the anisohydric species as drought events are anticipated to be more frequent and severe under global climate change. Identification of associated spectra among plant ecological strategies may increase understanding of how woody plants in temperate forests will respond to climate changes.","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":" ","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143404914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interactions between macro- and micro-climate: Effects on phenolic compound production in Nardus stricta at high elevations.

IF 4.2 3区生物学

Plant Biology Pub Date : 2025-02-13 DOI: 10.1111/plb.13776

X Moreira, J Durán, A Rodríguez, A Cao, M Correia, J Serôdio, S Rodríguez-Echeverría

{"title":"Interactions between macro- and micro-climate: Effects on phenolic compound production in Nardus stricta at high elevations.","authors":"X Moreira, J Durán, A Rodríguez, A Cao, M Correia, J Serôdio, S Rodríguez-Echeverría","doi":"10.1111/plb.13776","DOIUrl":"https://doi.org/10.1111/plb.13776","url":null,"abstract":"Phenolic compounds are key to plant defence, offering protection as antioxidants, UV shields, and antimicrobials. Their production is largely shaped by environmental conditions. It is believed that plants at lower elevations increase phenolic content to counter herbivory, while those at higher elevations rely on phenolics to manage abiotic stresses, such as climate variability. Microhabitat warming also affects phenolic levels, but responses differ, depending on broader climatic contexts: plants in warmer, lower-elevation environments show limited adaptability, whereas high-elevation plants demonstrate greater plasticity. Despite the importance of these environmental interactions, many small-scale abiotic studies lack sufficient spatial replication across broader gradients like elevation or latitude, while large-scale studies frequently overlook microscale factors. This study investigated the effects of macroclimate factors and microhabitat warming on phenolic production in Nardus stricta across five semi-natural grassland sites (1546-1875 m a.s.l.) in Portugal's Serra da Estrela. Warming was simulated using open-top chambers over two growing seasons, after which leaf samples were analysed for phenolic compounds, and soil nutrients were measured. The N. stricta plants at the highest elevation site contained significantly higher leaf flavonoid concentrations. Microhabitat warming led to a significant decrease in flavonoid concentrations, but only at the highest elevation site. These effects occurred independently of soil nutrient levels, suggesting direct thermal effects or stress responses might be involved. Our findings highlight the complex interactions between macro- and microenvironmental factors in shaping plant chemistry, underscoring critical considerations for plant resilience in the face of climate change. This understanding is essential for developing strategies to support plant and ecosystem adaptation to changing climates.","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":" ","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143404915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Within-plant variation in chemical defence of Erysimum cheiranthoides does not explain Plutella xylostella feeding preference.

IF 4.2 3区生物学

Plant Biology Pub Date : 2025-02-12 DOI: 10.1111/plb.13777

K Wang, T Züst

{"title":"Within-plant variation in chemical defence of Erysimum cheiranthoides does not explain Plutella xylostella feeding preference.","authors":"K Wang, T Züst","doi":"10.1111/plb.13777","DOIUrl":"https://doi.org/10.1111/plb.13777","url":null,"abstract":"Plants invest a substantial fraction of their resources into defence against herbivores, with the highest levels of defence often allocated only to the most valuable tissues. Plants in the genus Erysimum (Brassicaceae) have evolved the ability to produce novel cardenolides, in addition to ancestrally conserved glucosinolates. While these plants co-express both defences, differences in tissue-specific expression might represent an effective cost-saving strategy. Larvae of the glucosinolate-resistant diamondback moth Plutella xylostella occasionally feed on Erysimum cheiranthoides but tend to avoid younger leaves. Here, we predict that caterpillar feeding preference is shaped by variations in cardenolide levels. Thus, we quantified within-plant variations in defence and nutritional traits of vegetative or early reproductive plants and performed feeding assays to evaluate the relative importance of cardenolides. In accordance with optimal defence theory (ODT), the youngest leaves contained the most nutrients and had highest levels of cardenolides, glucosinolates and trichomes, with more extreme within-plant differences found in reproductive plants. Caterpillars consistently avoided the well-defended youngest leaves, both on whole plants and detached leaf discs. Surprisingly, neither experimental addition (external application) nor removal (CRISPR-Cas9 knockout) of cardenolides significantly affected caterpillar feeding preference. Physical and chemical defences, including cardenolides, co-vary within E. cheiranthoides to maximize defence of youngest leaves. While P. xylostella clearly responds to some of these traits, the prominent cardenolide defence appears to lack potency against this specialist herbivore. Nonetheless, the careful regulation and re-mobilization of cardenolides to younger leaves during plant development suggests an important role in plant functioning.","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":" ","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143397591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Omic characterisation of multi-component defences against the necrotrophic pathogen Pyrenophora tritici-repentis in wheat.

IF 4.2 3区生物学

Plant Biology Pub Date : 2025-02-07 DOI: 10.1111/plb.13746

L C Ferreira, F M Santana, S M M Scagliusi, M Beckmann, L A J Mur

{"title":"Omic characterisation of multi-component defences against the necrotrophic pathogen Pyrenophora tritici-repentis in wheat.","authors":"L C Ferreira, F M Santana, S M M Scagliusi, M Beckmann, L A J Mur","doi":"10.1111/plb.13746","DOIUrl":"https://doi.org/10.1111/plb.13746","url":null,"abstract":"Tan Spot disease is caused by the necrotrophic pathogen Pyrenophora tritici-repentis (Ptr) and poses a significant threat to global wheat production. Therefore, novel sources of resistance need to be identified, coupled with a fuller mechanistic understanding of host responses to Ptr. Herein, we characterise the interaction between a ToxA-positive Ptr strain and parental wheat lines from a multiparent advanced generation intercross (MAGIC) population. Genotypes displaying moderate resistance ('Robigus') or susceptibility ('Hereward') to Ptr challenge were identified and characterised through histological, metabolomic, and transcriptomic approaches. Histological investigations indicated the prominence of papilla-based defences in Robigus. Transcriptomic analyses could link this to the expression of barrier-related genes i.e. actin polymerisation, callose deposition, vesicle trafficking, and cellulose synthesis. Inhibiting actin polymerisation with cytochalasin E increased lesion numbers but did not augment lesion growth, suggesting the deployment of other defence mechanisms. These may be influenced by auxin, as its exogenous application exacerbated symptom development. Transcriptomic and metabolomic analyses in Hereward following challenge with Ptr suggested shifts in primary metabolism, affecting glycolysis, the TCA cycle, and the γ-aminobutyric acid (GABA) shunt. Activation of salicylic acid (SA)-associated genes, including NPR1 and WRKY33, was specific to Hereward, and exogenous SA increased susceptibility to Ptr in both genotypes. This study suggests barrier defences could be effective against Ptr as well as a lack of susceptibility factors like SA or the appropriate processing of IAA. These findings offer potential avenues for enhancing wheat resistance to Ptr.","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":" ","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143370170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitigation of salinity stress in salt-sensitive rice seedlings via phytohormone synthesis, antioxidant defence enhancement, and ion balance regulation induced by 5-aminolevulinic acid-producing purple non-sulfur bacteria.

IF 4.2 3区生物学

Plant Biology Pub Date : 2025-02-03 DOI: 10.1111/plb.13773

L S Sundar, J-Y Wu, Y-K Tu, H-W Chen, Y-Y Chao

{"title":"Mitigation of salinity stress in salt-sensitive rice seedlings via phytohormone synthesis, antioxidant defence enhancement, and ion balance regulation induced by 5-aminolevulinic acid-producing purple non-sulfur bacteria.","authors":"L S Sundar, J-Y Wu, Y-K Tu, H-W Chen, Y-Y Chao","doi":"10.1111/plb.13773","DOIUrl":"https://doi.org/10.1111/plb.13773","url":null,"abstract":"Salt stress, intensified by climate change, is a significant threat to rice production, a vital staple for over half the world's population. This makes addressing salt stress in rice cultivation a pressing issue. This study investigates the role of PNSB as a biostimulant in enhancing salinity tolerance of salt-sensitive rice seedlings, addressing existing gaps in knowledge on physiological and biochemical impacts under saline stress. We inoculated salt-sensitive rice seedlings with PNSB under 80 mmol NaCl stress in a controlled environment. After a 5-day treatment, we conducted biochemical and physiological analyses. Salinity stress induced oxidative stress in salt-sensitive rice seedlings. However, application of 5-ALA-producing PNSB mitigated stress, elevated 5-ALA in shoots by 23%, roots by 190.5%, and chlorophyll content by 105.0%. PNSB treatment also reduced superoxide radicals (O2 •-) and H2O2 by 26.7% and 38.7%, respectively, related to increased activity of the antioxidant enzymes, SOD (142.9%) and APX (41.8%). This led to lower electrolyte leakage (25.2%) and MDA (17.4%), indicating reduced ROS. Additionally, proline and soluble sugar content decreased by 29.2% and 72.5%, respectively. PNSB treatment also reduced sodium to potassium ion content in both shoots (31.2%) and roots (27.4%) of salt-stressed rice seedlings. These findings suggest that PNSB may facilitate nutrient solubilization and ion balance, thereby mitigating the adverse effects of salinity, with potential implications for sustainable agricultural practices to improve crop yield in saline environments. Future research should focus on elucidating the specific biochemical pathways involved in PNSB-mediated stress tolerance and exploring their application across diverse crop species and varying stress conditions.","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":" ","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a novel multivariate germination index to quantify seed hydration memory.

IF 4.2 3区生物学

Plant Biology Pub Date : 2025-02-03 DOI: 10.1111/plb.13766

A T Lima, T S Almeida, D G de Santana, M V Meiado

{"title":"Development of a novel multivariate germination index to quantify seed hydration memory.","authors":"A T Lima, T S Almeida, D G de Santana, M V Meiado","doi":"10.1111/plb.13766","DOIUrl":"https://doi.org/10.1111/plb.13766","url":null,"abstract":"In recent decades, the concept of memory has gained significant attention in plant ecophysiology research. When memory is expressed during germination, it is referred to as 'seed hydration memory'. Although numerous metrics relate to different aspects of germination, the literature lacks a method for quantifying seed hydration memory. This study aimed to develop a Germination Memory Index (GMI); a mathematical tool designed to quantify seed memory. The GMI enables comparisons of different native or cultivated species germinating under various conditions following discontinuous hydration. We tested the GMI using data from an experiment that evaluated germination of Cereus jamacaru seeds subjected to water deficit after undergoing hydration and dehydration cycles (HD cycles). The index is calculated as the arithmetic mean of gains, if any, in germination capacity, expressed as germinability, that is time to reach 50% germination, mean germination rate, uniformity of germination, synchrony and germination uncertainty, obtained from discontinuous hydration of the seeds. The memory ranges from 0 ≤ GMI ≤1. Gains are observed when HD cycles enhance germination capacity, germination rate and synchrony while reducing time to reach 50% germination, uniformity and uncertainty compared to their reference values. The absence of memory occurs when GMI ≤0, indicating that the germination process is favourable without undergoing HD cycles. When germination only occurs after discontinuous hydration, GMI = 1. Using the GMI, we can accurately measure gains in germinative behaviour provided by HD cycles, as this index reflects changes not only in germination capacity but also in germination time, speed, uniformity, synchrony and uncertainty.","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":" ","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Female-biased nectar production is associated with the Darwin's inflorescence syndrome

IF 4.2 3区生物学

Plant Biology Pub Date : 2025-01-27 DOI: 10.1111/plb.13768

M. M. Strelin, S. S. Gavini, M. A. Aizen

引用次数: 0