Plant Biology最新文献_第2页

A dynamic epigenetic perspective on above- and belowground phenotypic responses to drought: Insights from global DNA methylation in Erodium cicutarium. 对干旱的地上和地下表型反应的动态表观遗传学观点：来自黄牡丹全球DNA甲基化的见解。

IF 3.6 3区生物学

Plant Biology Pub Date : 2025-09-15 DOI: 10.1111/plb.70110

C Alonso, M Medrano, C M Herrera

{"title":"A dynamic epigenetic perspective on above- and belowground phenotypic responses to drought: Insights from global DNA methylation in Erodium cicutarium.","authors":"C Alonso, M Medrano, C M Herrera","doi":"10.1111/plb.70110","DOIUrl":"https://doi.org/10.1111/plb.70110","url":null,"abstract":"There is mounting evidence that plant responses to environmental stress are mediated by epigenetic factors, including DNA methylation. Understanding relationships between DNA methylation, plant development and individual fitness in contrasting environments is key to uncover potential impacts of epigenetic regulation on plant adaptation. Here, we used an experimental approach combining controlled alteration of epigenetic features with exposure to stress. Two provenances of Erodium cicutarium were exposed to a demethylating agent (5-azacytidine) and recurrent drought, and effects on above- and belowground phenotypic traits related to early development, phenology and fitness assessed. Application of 5-azacytidine significantly reduced DNA methylation in leaf and root tissues. This slowed plant development, delayed flowering, and reduced the number of inflorescences produced, independent of water regime. Recurrent drought reduced final above- and belowground biomass and inflorescence production, regardless of the 5-azacytidine exposure. Increased fruit and seed-set were the only adaptations to drought in E. cicutarium, together with an increased number of flowers per inflorescence in water-stressed plants previously treated with 5-azacytidine. Epigenetic effects can desynchronize plant growth, flowering and senescence in both favourable and adverse environments. Future studies should focus on understanding intraspecific variation in ability to change the plant methylome in response to stress, and transgenerational transmission of such responses.","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145068647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterologous expression of DRB0118 gene from Deinococcus radiodurans confers abiotic stress tolerance in soybean. 耐辐射球菌DRB0118基因的异源表达使大豆具有非生物抗逆性。

IF 3.6 3区生物学

Plant Biology Pub Date : 2025-09-15 DOI: 10.1111/plb.70111

K Zang, L Zhou, P Wang, W Guo, X Su, M Afridi, J Wang, H Guo, H Cheng

{"title":"Heterologous expression of DRB0118 gene from Deinococcus radiodurans confers abiotic stress tolerance in soybean.","authors":"K Zang, L Zhou, P Wang, W Guo, X Su, M Afridi, J Wang, H Guo, H Cheng","doi":"10.1111/plb.70111","DOIUrl":"https://doi.org/10.1111/plb.70111","url":null,"abstract":"Soybean (Glycine max) is a globally important crop for oil and protein production, but its growth and yield are severely affected by abiotic stresses, such as drought and salinity. We investigated subcellular localization of the DRB0118 gene from Deinococcus radiodurans, and phenotypic, physiological and biochemical indicators of DRB0118 overexpressing soybean plants under salt and drought stresses. Combined with transcriptome data, the results showed that overexpression of DRB0118 improved salt and drought tolerance of soybean. Subcellular localization revealed that the DRB0118 protein is localized in the nucleus and cell membrane. Overexpressed DRB0118 soybean lines had significantly improved survival under drought and salt stress, accompanied by enhanced superoxide dismutase (SOD) and peroxidase (POD) activity, reduced malondialdehyde (MDA) content, and lower reactive oxygen species (ROS) accumulation, as well as tighter closure of the stomatal aperture and a stronger root system. Transcriptome profiling further revealed that DRB0118 upregulated photosynthesis-related pathways under drought stress, and flavonoid biosynthesis under salt stress, both critical for mitigating oxidative damage. These findings highlight DRB0118 as a promising candidate gene for engineering crops with enhanced resilience to abiotic stresses. We discuss the potential mechanism of overexpressing DRB0118-enhanced salt and drought tolerance in soybean, including changes in antioxidants, stomata and roots, and enrichment of photosynthetic and flavonoid synthesis pathways.","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145068654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Season over plant sex: drivers of leaf damage and plant defence in a dioecious Mediterranean shrub. 季节对植物性别的影响：一种雌雄异株地中海灌木叶片损伤和植物防御的驱动因素。

IF 3.6 3区生物学

Plant Biology Pub Date : 2025-09-15 DOI: 10.1111/plb.70115

E Valdés-Correcher, G Calvo, C Rigueiro, B Lago-Núñez, P Jordano, X Moreira

{"title":"Season over plant sex: drivers of leaf damage and plant defence in a dioecious Mediterranean shrub.","authors":"E Valdés-Correcher, G Calvo, C Rigueiro, B Lago-Núñez, P Jordano, X Moreira","doi":"10.1111/plb.70115","DOIUrl":"https://doi.org/10.1111/plb.70115","url":null,"abstract":"In dioecious plants, females often prioritize reproduction over growth, potentially investing more in defence, while males grow faster but allocate fewer resources to defence, making them more susceptible to herbivory. Recent studies challenge this view, showing that males may grow more slowly and sometimes invest equally or more in defence. Variability in sex-specific herbivory and defence strategies may stem from seasonal shifts in resource allocation, with females prioritizing growth early in the season and reproduction later. These changes complicate herbivory patterns, necessitating research that considers temporally dynamic factors. This study investigated plant sex influence on herbivory and defence mechanisms in Pistacia lentiscus over the course of a year in Doñana National Park. We assessed insect herbivory and leaf traits linked to herbivore resistance, including phenolic compounds and specific leaf area (SLA), in 100 P. lentiscus plants (53 female, 47 male) at two sites during early and late seasons. Herbivory was higher in males than females and increased late in the season. A significant interaction between plant sex and season revealed that males experienced more herbivory late in the season, while there was no significant difference in the early season. Leaf phenolic concentration and SLA were higher early in the season, but these traits were not influenced by plant sex or the interaction between plant sex and season. Moreover, plant sex and season effects on herbivory remained significant even after controlling for leaf phenolics and SLA as covariates, indicating that these traits do not fully explain the observed differences in herbivory across sexes and seasons. Overall, our findings highlight the complex interplay between seasonality and plant sex in shaping herbivory and defence strategies, emphasizing the need to consider temporal dynamics when studying plant-herbivore interactions in dioecious species.","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145068620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative analysis of plastid genomes from allopolyploid Tragopogon miscellus and its diploid parents. 异源多倍体杂角兔及其二倍体亲本质体基因组的比较分析。

IF 3.6 3区生物学

Plant Biology Pub Date : 2025-09-15 DOI: 10.1111/plb.70109

U Mukhtar, S C Newmarch, R C Winkworth, P S Soltis, D E Soltis, J A Tate

{"title":"Comparative analysis of plastid genomes from allopolyploid Tragopogon miscellus and its diploid parents.","authors":"U Mukhtar, S C Newmarch, R C Winkworth, P S Soltis, D E Soltis, J A Tate","doi":"10.1111/plb.70109","DOIUrl":"https://doi.org/10.1111/plb.70109","url":null,"abstract":"Tragopogon is a model system for the study of recent, recurrent, and reciprocal allopolyploid formation. Recent research has focused on the fates of nuclear genes duplicated in the allopolyploid T. miscellus relative to the parental diploids, T. dubius and T. pratensis. In contrast, little attention has been given to organellar genomes, which interact with the duplicated nuclear genomes via their gene products. Here we reconstructed plastid genomes (plastomes) for representatives of these three species to investigate their structure and variability among natural and synthetic allopolyploids. Genomic libraries were Illumina-sequenced for several individuals of the allopolyploid T. miscellus and its diploid parents. Whole plastomes were assembled from skimmed data with comparative analyses used to quantify structural and nucleotide variation. Tragopogon plastomes have a typical quadripartite structure and are similar in size to those of other Asteraceae. The 12 plastomes were highly similar, sharing ~99.5%-100% identity. In all but one case, the plastome sequence for each of the polyploids was most similar to that of its expected maternal parent. The exception involved a polyploid that unexpectedly had a T. dubius plastome type, likely as the result of backcrossing with its presumed paternal parent. Such backcrossing events may have contributed to the demise of this polyploid population. Plastome sequences can be used to infer the maternal origins of polyploids as well as investigate ongoing population-level dynamics. More fully assessing plastome variation across the geographic distribution of polyploids and their diploid progenitors may provide additional insights into polyploid formation, population dynamics, and subsequent evolution.","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145068610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leaf phosphorus allocation patterns and photosynthetic phosphorus-use-efficiency in phosphorus-limited conditions. 限磷条件下叶片磷分配模式与光合磷利用效率

IF 3.6 3区生物学

Plant Biology Pub Date : 2025-09-15 DOI: 10.1111/plb.70114

L-G Li, Q-M Wang, J Pang, N-J Ma, J He, H Lambers

{"title":"Leaf phosphorus allocation patterns and photosynthetic phosphorus-use-efficiency in phosphorus-limited conditions.","authors":"L-G Li, Q-M Wang, J Pang, N-J Ma, J He, H Lambers","doi":"10.1111/plb.70114","DOIUrl":"https://doi.org/10.1111/plb.70114","url":null,"abstract":"The roles of leaf phosphorus (P) fractions in leaf photosynthetic P-use-efficiency (PPUE) under low soil P, and differences, if any, between legume and non-legume species are largely unknown. We investigated 24 crop species, 13 legumes and 11 non-legumes, for leaf PPUE and P acquisition strategies under low plant-available soil P. Legume species had a lower PPUE (38%), photosynthetic rate per area (Aarea; 38%) and per mass (Amass; 32%), but higher leaf P concentration (19%) than non-legume species. Legume species had a significantly higher nucleic acid P concentration, while percentage of nucleic acid P was higher, but percentage of inorganic P and metabolite P were lower in legumes than in non-legumes. Legume species had significantly greater total root length and carboxylate release than non-legume species. PPUE showed a positive correlation with Aarea, Amass, percentage of lipid P and metabolite P, but a negative correlation with concentrations of inorganic P, nucleic acid P, and leaf P. There was contrasting P-use-efficiency and P acquisition capacities between legume and non-legume species; high photosynthesis rate was associated with a high metabolite P concentration, but low nucleic acid P concentrations were related to a high PPUE. A P mining strategy was associated with a high leaf P concentration and low PPUE. This study provides insight into P use and P acquisition strategies and their correlations among 24 crop species grown under low plant-available soil P conditions.","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145068681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tip-to-base water storage strategies and their relationship to hydraulic safety in two temperate conifer species. 两种温带针叶树的尖基蓄水策略及其与水力安全的关系。

IF 3.6 3区生物学

Plant Biology Pub Date : 2025-09-14 DOI: 10.1111/plb.70101

T Savi, G Petit, D Zambonini, L N Biruk, S Rosner

{"title":"Tip-to-base water storage strategies and their relationship to hydraulic safety in two temperate conifer species.","authors":"T Savi, G Petit, D Zambonini, L N Biruk, S Rosner","doi":"10.1111/plb.70101","DOIUrl":"https://doi.org/10.1111/plb.70101","url":null,"abstract":"The axial co-variation of xylem anatomical traits is well documented, but lacks a deeper understanding of the tip-to-base dynamics of wood capacitance and resistance to embolism formation for assessing the performance of forest trees under drought stress. For the first time, relative water loss (RWL) curves were generated from wood sampled along the entire length of two mature conifer trees, spanning from the tip of the canopy to the base of the trunk. These measurements were conducted alongside hydraulic vulnerability curves. Parameters related to wood water retention capacity and safety/efficiency of the hydraulic system were extracted. The results revealed significant changes in wood capacitance, resistance to embolism formation and maximum hydraulic conductivity along the gradient from the tree tip to the base, with the most pronounced variation occurring within the first 200 apical centimetres. Resistance to embolism formation and wood capacitance were notably greater at the crown periphery compared to the stem base, with lower water potentials (Ψ) driving 20%, 50%, and 80% loss of hydraulic conductivity, accompanied by a higher release of wood capacitive water volume at the P50 threshold. The strong correlation between relative water loss and conductivity loss highlights the promising potential of traits derived from RWL curves as efficient and rapid indicators for assessing drought sensitivity. This research sheds light on the potential link between axial variation in xylem anatomical traits, drought-induced embolism vulnerability, and wood capacitance, with important implications for investigating plant responses to climate change.","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145058107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mycorhizomes and ectomycorrhizal associations facilitate a mycoheterotrophic nutritional mode in the green orchid Cymbidium kanran. 菌根和外生菌根的结合促进了绿兰蕙兰的真菌异养营养模式。

IF 3.6 3区生物学

Plant Biology Pub Date : 2025-09-14 DOI: 10.1111/plb.70103

K Suetsugu, H Okada

{"title":"Mycorhizomes and ectomycorrhizal associations facilitate a mycoheterotrophic nutritional mode in the green orchid Cymbidium kanran.","authors":"K Suetsugu, H Okada","doi":"10.1111/plb.70103","DOIUrl":"https://doi.org/10.1111/plb.70103","url":null,"abstract":"Nutritional modes in mature orchids range from complete autotrophy to full mycoheterotrophy. Within the genus Cymbidium, interspecific variation in the degree of mycoheterotrophy has been documented, yet intraspecific variation remains poorly understood. Intriguingly, some green Cymbidium species often possess mycorhizomes (coralloid rhizomes), structures commonly found in fully mycoheterotrophic orchids, and morphologically similar to fully mycoheterotrophic seedlings (protocorms). This study examines Cymbidium kanran individuals with and without mycorhizomes to assess whether these specialized subterranean structures enhance fungal carbon acquisition through the use of stable isotope analyses (δ13C and δ15N) and high-throughput metabarcoding techniques. Cymbidium kanran with mycorhizomes exhibit significantly elevated δ13C and δ15N compared to autotrophic reference species and congeneric individuals lacking mycorhizomes. The mycorhizome-bearing plants are partially mycoheterotrophic, obtaining nearly half of their carbon from fungi. The degree of mycoheterotrophy is similar to that of other partially mycoheterotrophic Cymbidium species, such as C. lancifolium and C. goeringii. Fungal community profiling revealed that mycorhizome-bearing C. kanran individuals mostly associate with ectomycorrhizal fungi, such as Sebacinaceae, whereas individuals lacking mycorhizomes primarily recruit non-ectomycorrhizal rhizoctonia fungi. Notably, this morphological and nutritional plasticity parallels patterns observed in other orchid lineages, such as Cremastra and Oreorchis, which associate with wood-decay fungi. The findings strongly suggest that persistent protocorm-like mycorhizomes enhance fungal carbon uptake in partially mycoheterotrophic orchids associated not only with wood-decaying fungi but also with ectomycorrhizal fungi. These insights expand understanding of orchid nutritional strategies and highlight how intraspecific morphological plasticity may contribute to the evolution of mycoheterotrophy.","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145058178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Environmental and phytohormone modulation of organ-specific specialized metabolite profiles in the dryland tree Erythrina velutina. 旱地树木赤藓器官特异性代谢物谱的环境和植物激素调节。

IF 3.6 3区生物学

Plant Biology Pub Date : 2025-09-08 DOI: 10.1111/plb.70104

D S Chacon, B Bonilauri, C T da Costa, J Vilasboa, M Koetz, L Pinto, J A S Zuanazzi, R B Giordani, A G Fett-Neto

{"title":"Environmental and phytohormone modulation of organ-specific specialized metabolite profiles in the dryland tree Erythrina velutina.","authors":"D S Chacon, B Bonilauri, C T da Costa, J Vilasboa, M Koetz, L Pinto, J A S Zuanazzi, R B Giordani, A G Fett-Neto","doi":"10.1111/plb.70104","DOIUrl":"https://doi.org/10.1111/plb.70104","url":null,"abstract":"Erythrina velutina is a tree that thrives in the shallow rocky soils of the dry and hot Caatinga, a unique Brazilian biome. It is rich in specialized metabolites with medicinal properties. Indeed, alkaloids and flavonoids are phytochemical markers of the genus. Our previous studies identified key biochemical and molecular targets in biosynthesis of these metabolites in E. velutina, including phytohormone signalling pathways and responses to environmental stressors. However, the role of these signalling molecules and external factors in modulating the tree natural product (NP) profiles remains unexplored. In this study, seedlings of E. velutina were subjected to environmental stress (heat, ultraviolet radiation, drought, salinity, mechanical damage) and phytohormone exposure (methyl jasmonate, salicylic acid, nitric oxide, abscisic acid). Leaves and roots were collected after 2 and 4 days of treatment for HPLC-DAD and chemometric analyses. The most prominent factors that increased accumulation of major metabolites were nitric oxide, drought, heat, ultraviolet radiation, and methyl jasmonate. The analyses revealed both organ- and temporal-specific metabolite profiles, as well as some typically shared features. Both phytoanticipin and phytoalexin-like metabolite profiles were recorded, with prevalence of the former. The results shed light on how the above factors affect metabolic tuning in E. velutina. Moreover, the generated datasets will be useful in selecting individual compounds for detailed functional investigation, as well as for directing chemical profiles towards known and novel metabolites of interest in this species.","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145022537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional trade-offs and ecological correlates of seed oil traits in alpine ecosystems. 高山生态系统种子油性状的功能权衡与生态关联

IF 3.6 3区生物学

Plant Biology Pub Date : 2025-09-04 DOI: 10.1111/plb.70097

C Espinosa Del Alba, A Mondoni, S Baizán, E Fernández-Pascual, B Jiménez-Alfaro

{"title":"Functional trade-offs and ecological correlates of seed oil traits in alpine ecosystems.","authors":"C Espinosa Del Alba, A Mondoni, S Baizán, E Fernández-Pascual, B Jiménez-Alfaro","doi":"10.1111/plb.70097","DOIUrl":"https://doi.org/10.1111/plb.70097","url":null,"abstract":"Seed oil content is a crucial energy reserve in angiosperms, with effects on biological functions and plant fitness, playing an important role in seed protection from environmental conditions. However, the eco-evolutionary role of seed oil traits in natural ecosystems is largely unexplored. We studied functional trade-offs and ecological drivers of seed oil traits in 47 species of an alpine flora. We analysed seed oil content and composition - relative proportion of unsaturated and saturated fatty acids (UFA/SFA) - and their relationships with seed mass, seed longevity, and germination timing. We also tested the response of seed oil traits to species' ecological preferences along microclimatic gradients. Seed oil content ranged from 1% to 34.2%, with the main variation explained by the UFA/SFA ratio, and little variation across altitude. Seed oil content significantly influenced seed longevity but had no relationships to seed mass and germination. Contrary to expectations, microclimatic gradients did not affect seed oil content or composition. All analyses showed strong phylogenetic constraints on seed oil traits. Seed oil traits in alpine species do not differ from those of other herbaceous species. A major trade-off between seed oil and seed longevity may help in understanding species regeneration in alpine soils and defining protocols for ex-situ conservation. The evolutionary conservatism of seed oil traits likely prevents seed-based selection of alpine species across altitudinal and microclimatic gradients.","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144999380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-wide identification of xyloglucan endotransglucosylase/hydrolase genes in almond and PdXTH2 overexpression induces cell structural disassembly and promotes programmed cell death progression. 杏仁和PdXTH2过表达木糖葡聚糖内转葡萄糖酶/水解酶基因的全基因组鉴定诱导细胞结构解体并促进程序性细胞死亡进程。

IF 3.6 3区生物学

Plant Biology Pub Date : 2025-09-04 DOI: 10.1111/plb.70098

D Zhang, B Zeng, X Zeng, Y He, X Liu, Z Yu

{"title":"Genome-wide identification of xyloglucan endotransglucosylase/hydrolase genes in almond and PdXTH2 overexpression induces cell structural disassembly and promotes programmed cell death progression.","authors":"D Zhang, B Zeng, X Zeng, Y He, X Liu, Z Yu","doi":"10.1111/plb.70098","DOIUrl":"https://doi.org/10.1111/plb.70098","url":null,"abstract":"The xyloglucan endotransglucosylase/hydrolase (XTH) gene family plays a crucial role in fruit development and texture regulation. We used the telomere-to-telomere genome of 'Wanfeng' almond to identify and analyse the XTH gene family, examining protein physicochemical properties, phylogenetic relationships, protein conserved motifs, gene structure, gene replication, and expression patterns. We identified 23 PdXTH family members through the telomere-to-telomere level genome of 'Wanfeng' almond. These genes exhibited significant variations in protein physicochemical properties, gene structure, and sequence conservation. PdXTH genes were predominantly distributed in the I/II subfamily and demonstrated high sequence conservation and gene structure similarity. Gene duplication events contributed to an increase in PdXTH family members. Analysis of cis-acting elements suggest that PdXTH genes are involved in almond growth and development, stress response, and hormone regulation. Expression patterns revealed that PdXTH genes display significant tissue-specific expression. PdXTH genes had notable temporal gradient changes during fruit development and may function at different development stages. The sustained high expression of PdXTH genes throughout almond fruit development represents a potential mechanism for maintaining fruit firmness. Furthermore, transgene studies confirmed that PdXTH2 has a regulatory role in programmed cell death. PdXTH2 is localized to the cell membrane. PdWRKY24 and PdbHLH92 can directly bind to the PdXTH2 promoter region and positively regulate its transcriptional activity. In conclusion, this study provides new insights into the role of the XTH family in plant physiology.","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144999294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0