IF 3.1 4区生物学

Adipocyte Pub Date : 2026-12-01 Epub Date: 2026-01-18 DOI: 10.1080/21623945.2025.2610544

Seyede Fatemeh Heydari, Mehrdad Moosazadeh Moghaddam, Soyar Sari, Mohammad Heiat

{"title":"Physical differentiation of adipose-derived mesenchymal stem cells into pancreatic beta-like cells using cell-imprinting and evaluation of insulin production.","authors":"Seyede Fatemeh Heydari, Mehrdad Moosazadeh Moghaddam, Soyar Sari, Mohammad Heiat","doi":"10.1080/21623945.2025.2610544","DOIUrl":"10.1080/21623945.2025.2610544","url":null,"abstract":"Stem cell-based therapies are emerging as a promising treatment for diabetes by differentiating these cells into insulin-producing cells (IPCs). However, using growth factors for differentiation has always been challenging. Physical differentiation of stem cells presents a promising approach to reduce reliance on chemical growth factors. One method of physical cell differentiation is cell imprinting. This study aimed to physically induce the differentiation of rat adipose-derived mesenchymal stem cells (rADSCs) into β-like cells using the cell-imprinting technique. For this purpose, RIN-5F cells were used to transfer their geometry and cell-specific topographies to a polydimethylsiloxane (PDMS) substrate. After cell imprinting, the rADSCs were seeded on the substrate, and their differentiation into β-like cells was evaluated after 14 and 21 days by assessing insulin production using dithizone staining and ELISA, as well as real-time PCR and immunocytochemistry (ICC) for expression analysis of the genes effective in cell differentiation into β-like cells, including PDX1, NKX6.1, NGN3, and insulin. The results of dithizone staining and ELISA confirmed insulin secretion by differentiated cells compared to stem cells (p ≤ 0.05). Real-time PCR and ICC results showed that after 21 days, the differentiated cells expressed key β-cell genes significantly more than stem cells (p ≤ 0.05).","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"15 1","pages":"2610544"},"PeriodicalIF":3.1,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12818817/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145996946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Super-enhancer-associated miR-1260b coordinates adipogenesis and metabolic programming in human adipose stem cells. 超增强子相关的miR-1260b在人类脂肪干细胞中协调脂肪形成和代谢程序。

IF 3.1 4区生物学

Adipocyte Pub Date : 2026-12-01 Epub Date: 2026-03-13 DOI: 10.1080/21623945.2026.2638071

Sen Li, Shuhui Ji, Zihan Yu, Haiyang Liu, Jianxin Wu

{"title":"Super-enhancer-associated miR-1260b coordinates adipogenesis and metabolic programming in human adipose stem cells.","authors":"Sen Li, Shuhui Ji, Zihan Yu, Haiyang Liu, Jianxin Wu","doi":"10.1080/21623945.2026.2638071","DOIUrl":"10.1080/21623945.2026.2638071","url":null,"abstract":"Obesity and its metabolic complications represent a major and growing public health burden, for which effective therapeutic strategies remain limited. Although super-enhancers are recognized as key regulators of cell identity and adipogenesis, the roles of super-enhancer- microRNAs in obesity and adipocyte dysfunction remain insufficiently characterized. Here, we identify miR-1260b as a super-enhancer-associated miRNA that influences human adipogenesis. Analysis of adipose-related datasets from SEdb 2.0 revealed that MIR1260B is consistently linked to a clustered SE region across multiple human adipose tissues. Integrated ATAC-seq and Hi-C analyses demonstrated progressive chromatin opening and dynamic enhancer-promoter interactions between this SE and the MIR1260B promoter during adipogenic differentiation. Functionally, miR-1260b overexpression markedly inhibited adipocyte differentiation of human adipose-derived stem cells. Quantitative proteomic profiling revealed that miR-1260b suppresses adipogenic and lipogenic programmes while activating lipid catabolic pathways. Notably, the MIR1260B-associated SE overlaps with a previously reported diabetes-associated SNP, and external clinical datasets indicate reduced miR-1260b levels in umbilical cord serum from children at increased risk of obesity. Together, these findings suggest that miR-1260b may function as a super-enhancer-associated regulator that inhibits adipogenesis and indicate that SE-informed multi-omics approaches can aid in identifying miRNA regulators relevant to metabolic disorders of obesity.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"15 1","pages":"2638071"},"PeriodicalIF":3.1,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12990932/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147442323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hypotonic induced volume activated chloride current in 3T3-L1 preadipocytes and its effect on cell differentiation. 低渗诱导3T3-L1前脂肪细胞体积激活氯电流及其对细胞分化的影响。

IF 3.1 4区生物学

Adipocyte Pub Date : 2026-12-01 Epub Date: 2026-04-07 DOI: 10.1080/21623945.2026.2649662

Hua Guan, Zhenghao Huang, Ziyue Wang, Aoqi Xiang, Lusha Zhang, Xiaochang Chen, Peihong Su, Rui Wang, Haifeng Zhang, Qi Yu

{"title":"Hypotonic induced volume activated chloride current in 3T3-L1 preadipocytes and its effect on cell differentiation.","authors":"Hua Guan, Zhenghao Huang, Ziyue Wang, Aoqi Xiang, Lusha Zhang, Xiaochang Chen, Peihong Su, Rui Wang, Haifeng Zhang, Qi Yu","doi":"10.1080/21623945.2026.2649662","DOIUrl":"https://doi.org/10.1080/21623945.2026.2649662","url":null,"abstract":"Volume-activated chloride current (VACC), the most common and abundant anion current in organisms, is involved in various physiological and pathological processes. However, its expression and characteristics in preadipocytes remain unclear. Whole-cell patch clamp technique was used to investigate the presence and properties of VACC in 3T3-L1 preadipocytes, as well as the effects of chloride channel blockers (NPPB, DIDS, and tamoxifen) on this current under different osmotic conditions. Additionally, 3T3-L1 preadipocytes were treated with the chloride channel blocker TMEM16A to observe its effect on cell differentiation. Hypotonic stimulation significantly induced whole-cell currents in 3T3-L1 preadipocytes, which exhibited obviously outward-rectifying and volume-activated characteristics. Under hypotonic stimulation, NPPB, DIDS, and tamoxifen all inhibited the current, with NPPB showing a significantly stronger inhibitory effect than DIDS and tamoxifen. Moreover, growth-arrested 3T3-L1 preadipocytes at 2 days post-confluence were successfully induced, with mRNA levels of C/EBPα, C/EBPβ, PPARγ and FABP4 peaking on day 3 and decreasing by day 14. Protein levels of PPARγ, perilipin 1 and LRRC8A were significantly upregulated during differentiation. Treatment with TMEM16A significantly reduced adipocyte lipid droplet formation and FABP4 mRNA/protein levels, but increased C/EBPα and PPARγ mRNA levels, as well as LRRC8A protein levels after 3 days. Collectively, our results clarify the characteristics of VACC in 3T3-L1 preadipocytes, confirm the inhibitory effect of chloride channel blockers on 3T3-L1 preadipocyte differentiation, fill the gap in the understanding of VACC in preadipocytes, and lay a preliminary experimental foundation for further exploring the role of VACC in adipocyte differentiation.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"15 1","pages":"2649662"},"PeriodicalIF":3.1,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147626953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

mTORC2 regulates lipid metabolism-driven TAMs via the PPAR-γ/CD36 pathway to promote liposarcoma progression. mTORC2通过PPAR-γ/CD36途径调节脂质代谢驱动的tam，促进脂肪肉瘤的进展。

IF 3.1 4区生物学

Adipocyte Pub Date : 2026-12-01 Epub Date: 2026-04-29 DOI: 10.1080/21623945.2026.2665903

Weihua Xiao, Jingdan Sheng, Chunjiao Liu, Junqiang Li, Peng Shu, Maofen Jiang, Min Wang, Ji He, Haifen Ma, Yaxin Ding

{"title":"mTORC2 regulates lipid metabolism-driven TAMs via the PPAR-γ/CD36 pathway to promote liposarcoma progression.","authors":"Weihua Xiao, Jingdan Sheng, Chunjiao Liu, Junqiang Li, Peng Shu, Maofen Jiang, Min Wang, Ji He, Haifen Ma, Yaxin Ding","doi":"10.1080/21623945.2026.2665903","DOIUrl":"10.1080/21623945.2026.2665903","url":null,"abstract":"Tumour-associated macrophages (TAMs) exert a pivotal function in tumour progression, and M2-type TAMs are closely linked to tumour-promoting functions. Mechanistic target of rapamycin complex 2 (mTORC2) may mediate TAM polarization and subsequent tumour development, yet their roles in liposarcoma (LPS) remain unclear. Macrophage polarization was assessed via flow cytometry for CD206. Western blot for Arg-1, Rictor, PPAR-γ, CD36, ACSL1 and CPT2, qPCR for IL-10, Arg-1 and Ym1, and ELISA for IL-10 secretion. Fatty acid metabolism was evaluated using free fatty acid (FFA) uptake assays and Oil Red O staining for intracellular lipid droplets. A Transwell assay was established to assess the effect of treated macrophages on LPS cell biology, and EdU assays were used for proliferation assessment. Transwell migration and invasion assays were utilized for assessing cellular motility. IL-4 induced RAW264.7 macrophages to undergo M2 polarization, characterized by upregulated CD206, Arg-1, and IL-10. During this process, mTORC2 was activated, promoting FFA uptake, lipid droplet accumulation, and fatty acid oxidation via the PPAR-γ/CD36 axis. Co-culture experiments showed that IL-4-polarized M2 macrophages enhanced LPS cell proliferation, migration, and invasion; these effects were inhibited by JR-AB2-011 and restored by LPA, confirming mTORC2-PPAR-γ/CD36-mediated TAMs drive LPS progression. mTORC2 regulates M2 TAM polarization and metabolic reprogramming via the PPAR-γ/CD36 pathway, thereby promoting LPS cell proliferation, migration, and invasion.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"15 1","pages":"2665903"},"PeriodicalIF":3.1,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13134416/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147759636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An acute temperature rise to 40°C inhibits free fatty acid uptake into white adipocytes. 急性温度升高到40°C会抑制游离脂肪酸进入白色脂肪细胞。

IF 3.1 4区生物学

Adipocyte Pub Date : 2026-12-01 Epub Date: 2026-02-11 DOI: 10.1080/21623945.2026.2626121

Federica Foti, Raoul S Schaepper, Daniel Konrad, Stephan Wueest

{"title":"An acute temperature rise to 40°C inhibits free fatty acid uptake into white adipocytes.","authors":"Federica Foti, Raoul S Schaepper, Daniel Konrad, Stephan Wueest","doi":"10.1080/21623945.2026.2626121","DOIUrl":"10.1080/21623945.2026.2626121","url":null,"abstract":"Fever reflects a physiological rise in body temperature accompanied by elevated production of adrenaline. The increased body temperature in fever is caused by shivering thermogenesis in skeletal muscle and non-shivering thermogenesis in brown adipose tissue (BAT), the latter being mediated by uncoupled oxidation of free fatty acids (FFAs). We hypothesized that an acute temperature rise to 40°C increases adrenalin-induced lipolysis in white adipocytes, thereby potentially providing FFAs as an energy substrate to sustain fever-induced thermogenesis in skeletal muscle and BAT. In 3T3-L1 and primary murine white adipocytes, isoproterenol-induced extracellular FFA accumulation was significantly increased at 40°C compared to 37°C. In contrast, isoproterenol-induced increase in extracellular glycerol concentrations and the protein levels of phosphorylated hormone sensitive lipase were comparable at both temperatures, suggesting a similar degree of lipolysis. Moreover, incubation at 40°C did neither increase isoproterenol-induced oxygen consumption nor intracellular FFA concentrations, indicating that the elevated extracellular FFA accumulation was not due to reduced intracellular consumption. Conversely, isoproterenol blunted FFA uptake into adipocytes to a significantly higher extent at 40°C compared to 37°C. Hence, an acute temperature rise to 40°C reduces FFA uptake into white adipocytes, thereby increasing extracellular FFA availability.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"15 1","pages":"2626121"},"PeriodicalIF":3.1,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12915836/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146163478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Disease-associated adipose browning: current evidence and perspectives. 疾病相关脂肪褐变：目前的证据和观点。

IF 3.1 4区生物学

Adipocyte Pub Date : 2026-12-01 Epub Date: 2026-01-07 DOI: 10.1080/21623945.2025.2610540

Xiyue Zhang, Xue Han, Jiesi Xu, Guoping Li

{"title":"Disease-associated adipose browning: current evidence and perspectives.","authors":"Xiyue Zhang, Xue Han, Jiesi Xu, Guoping Li","doi":"10.1080/21623945.2025.2610540","DOIUrl":"10.1080/21623945.2025.2610540","url":null,"abstract":"Brown and beige adipose tissue represent evolutionary adaptations in mammals, functioning as specialized thermogenic organs to maintain body temperature. Over the past two decades, researches have demonstrated that white adipose tissue (WAT) browning is an effective strategy to enhance energy expenditure. However, a growing body of evidence indicates that the browning process frequently occurs in a variety of chronic disease states, though its pathophysiological significance remains unclear. This review summarized evidence of pathological browning observed in human diseases and animal models, including breast cancer, colorectal cancer (CRC), clear cell renal cell carcinoma (ccRCC), kidney health, burn injury, atherosclerotic, SARS-CoV-2 and sepsis. Despite distinct pathological contexts, adipose tissue browning is consistently observed. This suggests that browning may not simply serve its classical metabolically protective role, but instead reflect an atypical response to pathological stress. It is currently unclear whether this is a compensatory mechanism by the organism in a diseased state or merely a byproduct of the disease process. Whether this response is adaptive or a cause of disease progression remains unresolved. Future research should therefore focus on identifying the triggers and functional outcomes of pathological browning to better understand adipocyte plasticity and its role in disease progression.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"15 1","pages":"2610540"},"PeriodicalIF":3.1,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12785212/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145909710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

cAMP-PKA/EPAC signaling pathways: crucial regulators of lipid homeostasis. cAMP-PKA/EPAC信号通路：脂质稳态的关键调节因子。

IF 3.1 4区生物学

Adipocyte Pub Date : 2026-12-01 Epub Date: 2026-01-07 DOI: 10.1080/21623945.2025.2603605

Caixia Chen, Hui Gao, Qi Tian, Junwei Cao

{"title":"cAMP-PKA/EPAC signaling pathways: crucial regulators of lipid homeostasis.","authors":"Caixia Chen, Hui Gao, Qi Tian, Junwei Cao","doi":"10.1080/21623945.2025.2603605","DOIUrl":"10.1080/21623945.2025.2603605","url":null,"abstract":"Adipose omeostasishomoeostasis is maintained through the precise coordination of lipogenesis, lipolysis, and adipocyte differentiation, with microenvironmental components dynamically regulating lipid metabolism. Even though the classical cAMP-PKA pathway has been well-characterized for its function in lipid metabolism by phosphorylating transcription factors and lipolytic enzymes, little is known about how it collaborates with elements of the adipose tissue microenvironment, such as immune cells and the vascular endothelium, especially in pathological situations like obesity. EPAC, a newly discovered cAMP effector, has shown new signalingsignallingsignalling signalling pathways in the immune and cardiovascular systems by activating small G proteins. However, there are important understanding gaps regarding its roles in adipose metabolism, namely adipocyte development, microenvironmental interaction, and the pathophysiology of metabolic diseases. By bringing together disparate studies on PKA and EPAC, this review provides the first comprehensive synthesis of the cAMP-PKA/EPAC dual signaling signalling signallingcins signalling network, filling in knowledge gaps. The reciprocal regulation between this signaling signalling signalling signalling network and the adipose microenvironment establishes a novel 'signaling-microenvironment-systemic metabolism' framework for understanding metabolic disorders, including obesity, diabetes, and hepatic steatosis. Pharmacological modulation of the PKA/EPAC signalingsignalling signalling signalling pathways may therefore represent a viable therapeutic approach for restoring adipose tissue homeostasis homoeostasis.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"15 1","pages":"2603605"},"PeriodicalIF":3.1,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12785204/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145931762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erianin inhibits 3T3-L1 adipocyte differentiation through downregulation of CCAAT-enhancer binding protein-α and peroxisome proliferator-activated receptor-γ, lipogenic genes and impairment of mitochondrial respiration. Erianin通过下调ccaat增强子结合蛋白-α和过氧化物酶体增殖物激活受体-γ、脂质基因和线粒体呼吸损伤来抑制3T3-L1脂肪细胞分化。

IF 3.1 4区生物学

Adipocyte Pub Date : 2026-12-01 Epub Date: 2025-12-19 DOI: 10.1080/21623945.2025.2601405

Hathaichanok Yimpreeda, Chayanee Laowittawat, Siraprapa Siritutsoontorn, Pinnara Rojvirat, Sarawut Kumphune, Sarawut Jitrapakdee

{"title":"Erianin inhibits 3T3-L1 adipocyte differentiation through downregulation of CCAAT-enhancer binding protein-α and peroxisome proliferator-activated receptor-γ, lipogenic genes and impairment of mitochondrial respiration.","authors":"Hathaichanok Yimpreeda, Chayanee Laowittawat, Siraprapa Siritutsoontorn, Pinnara Rojvirat, Sarawut Kumphune, Sarawut Jitrapakdee","doi":"10.1080/21623945.2025.2601405","DOIUrl":"10.1080/21623945.2025.2601405","url":null,"abstract":"Erianin, a natural bibenzyl compound, has recently garnered attention owing to its diverse biological activities. In the present study, we investigated the effects of Erianin on adipocyte differentiation, lipid metabolism, and mitochondrial respiration in murine 3T3-L1 cells. Cytotoxicity assays indicated that Erianin exhibited low toxicity towards preadipocytes at concentrations up to 200 μM. Treatment with 20 μM Erianin completely inhibited the differentiation of 3T3-L1 preadipocytes into mature adipocytes and reduced lipid droplets. Western blot analysis revealed that Erianin attenuated Akt and p38 MAPK signalling without inducing apoptosis, suppressed the expression of key pro-adipogenic transcription factors, C/EBPα and PPARγ during the early stages of differentiation. This suppression was accompanied by the downregulation of lipogenic enzymes, including acetyl-CoA carboxylase 1 (ACC1), fatty acid synthase (FASN), pyruvate carboxylase (PC) and 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR). While early-stage differentiation was robustly inhibited, higher concentrations (≥25 μM) were required to suppress terminal differentiation of immature adipocytes. This late-stage inhibition was accompanied by decreased expression of PPARγ, PC, and HMGCR, with minimal effects on ACC1 and FASN, suggesting a more modest role for Erianin in terminal adipogenesis. Assessment of mitochondrial metabolism of 3T3-L1 cells following 24-hour treatment of Erianin showed that it modestly impaired ATP-linked respiration, maximal respiration, spare respiratory capacity and intracellular ATP levels while basal respiration was unaffected. Collectively, these findings indicated that Erianin predominantly targets early adipogenic differentiation and mitochondrial bioenergetics.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"15 1","pages":"2601405"},"PeriodicalIF":3.1,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12721086/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145792858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The adiponectin/leptin ratio as a biomarker of adiposopathy and visceral adipose tissue accumulation: sex-specific mechanisms. 脂联素/瘦素比率作为脂肪病和内脏脂肪组织积累的生物标志物：性别特异性机制。

IF 3.1 4区生物学

Adipocyte Pub Date : 2026-12-01 Epub Date: 2026-04-07 DOI: 10.1080/21623945.2026.2653961

Aida Medina-Urrutia, Iván Torre-Villalvazo, Andrea Díaz-Villaseñor, Esteban Jorge-Galarza, Luz Graciela Cervantes-Pérez, Neftali Eduardo Antonio-Villa, Felipe Santibañez-Escobar, Gabriela Leal-Escobar, Brenda Marquina-Castillo, Froylan David Martínez-Sánchez, Juan Gabriel Juárez-Rojas

{"title":"The adiponectin/leptin ratio as a biomarker of adiposopathy and visceral adipose tissue accumulation: sex-specific mechanisms.","authors":"Aida Medina-Urrutia, Iván Torre-Villalvazo, Andrea Díaz-Villaseñor, Esteban Jorge-Galarza, Luz Graciela Cervantes-Pérez, Neftali Eduardo Antonio-Villa, Felipe Santibañez-Escobar, Gabriela Leal-Escobar, Brenda Marquina-Castillo, Froylan David Martínez-Sánchez, Juan Gabriel Juárez-Rojas","doi":"10.1080/21623945.2026.2653961","DOIUrl":"10.1080/21623945.2026.2653961","url":null,"abstract":"Adipose tissue (AT) dysfunction can lead to increased visceral AT (VAT) and metabolic damage. The adiponectin/leptin ratio (ALR) has been proposed as a biomarker of AT functionality. However, its participation in VAT accumulation and the impact of sex on these associations have not been evaluated. In an analytical cross-sectional study, 54 adults (29 male, 25 postmenopausal females, aged 30-70 years, BMI 19-31 kg/m2) were analysed. Anthropometric data, fasting serum samples, and subcutaneous AT (SAT) biopsies were obtained. Morpho-functional AT characteristics included ALR, adipocyte size, macrophage content and AT insulin resistance (ADIPO-IR). Using multivariate and mediation models, we evaluated the associations of SAT characteristics with systemic IR (TyG index), systemic inflammation (C-reactive protein), and VAT area. In postmenopausal females, ALR was inversely associated with adipocyte size, macrophage number, TyG index, CRP, and VAT area. SAT inflammation and ADIPO-IR were independently associated with VAT, and a mediation model suggested ALR as a possible precursor of VAT. Among males, ADIPO-IR and ALR were independently associated with VAT. These findings emphasize the importance of considering sex differences in the prevention and treatment strategies for metabolic diseases among Mexican-Mestizo populations, although these results should be confirmed by prospective studies.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"15 1","pages":"2653961"},"PeriodicalIF":3.1,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13064561/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147631951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The transcription elongation factors Spt4 and Spt6 promote dermal adipocyte differentiation. 转录延伸因子Spt4和Spt6促进真皮脂肪细胞分化。

IF 3.1 4区生物学

Adipocyte Pub Date : 2026-12-01 Epub Date: 2026-03-27 DOI: 10.1080/21623945.2026.2650002

Julian Gomez, Samiksha Mahapatra, Uyanga Batzorig, Ye Liu, Celia Fernández-Méndez, Neha Quadir, George L Sen

{"title":"The transcription elongation factors Spt4 and Spt6 promote dermal adipocyte differentiation.","authors":"Julian Gomez, Samiksha Mahapatra, Uyanga Batzorig, Ye Liu, Celia Fernández-Méndez, Neha Quadir, George L Sen","doi":"10.1080/21623945.2026.2650002","DOIUrl":"10.1080/21623945.2026.2650002","url":null,"abstract":"Regulation of adipogenesis has classically been viewed through the lens of transcription initiation driven by lineage defining transcription factors. In this study, we uncover transcription elongation as a critical and previously underappreciated regulatory layer controlling adipocyte cell fate. We demonstrate that the elongation factors Spt4 and Spt6 are indispensable for adipogenic differentiation, as their depletion severely impairs adipogenic gene induction and perilipin expression. Spt4 and Spt6 directly regulate the genes coding for core adipogenic transcription factors, including Cebpa, Pparg, Krox20, and Stat3, by promoting RNA polymerase II (Pol II) progression through their gene bodies. In the absence of these factors, Pol II becomes stalled at the transcriptional start sites of these adipogenic genes. These data support a post transcription initiation requirement for Spt4 and Spt6 in productive elongation rather than promoter loading. Our findings identify transcription elongation control as a key determinant of adipogenic fate.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"15 1","pages":"2650002"},"PeriodicalIF":3.1,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13034627/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147525286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adipocyte最新文献