IF 3.5 4区生物学

Adipocyte Pub Date : 2024-12-01 Epub Date: 2024-11-11 DOI: 10.1080/21623945.2024.2423723

Shaomin Shi, Ke Ding, Feng Chen, Mei Yang, Lihua Ni, Xiaoyan Wu

{"title":"Identification of hub genes in the crosstalk between type 2 diabetic nephropathy and obesity according to bioinformatics analysis.","authors":"Shaomin Shi, Ke Ding, Feng Chen, Mei Yang, Lihua Ni, Xiaoyan Wu","doi":"10.1080/21623945.2024.2423723","DOIUrl":"10.1080/21623945.2024.2423723","url":null,"abstract":"Diabetic nephropathy (DN) and obesity bring a huge burden to society. Obesity plays a crucial role in the progression of type 2 DN, but the pathophysiology remains unclear. Thus, we aimed the explore the association between type 2 DN and obesity using bioinformatics method. The gene expression profiles of type 2 DN (GSE96804) and obesity (GSE94752) were downloaded from the Gene Expression Omnibus (GEO) database. The differentially expressed genes (DEGs) were screened with the thresholds defined as |log2FC| ≥1 and P<0.05. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed. Subsequently, a protein-protein interaction network was constructed based on the STRING database. Hub genes were identified, and the co-expression network was constructed. Finally, the hub genes were verified in clinical samples of 24 patients by immunohistochemistry. A total of 17 common DEGs were identified. Finally, two overlapping hub genes were identified (CCL18, C1QC). C1QC has been verified in clinical specimens. Using bioinformatics methods, the present study analyzed the common DEGs and the potential pathogenic mechanisms involved in type 2 DN and obesity. C1QC was the hub gene. Further studies are needed to clarify the specific relationships among C1QC, type 2 DN and obesity.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"13 1","pages":"2423723"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11556279/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142611987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An optimised protocol for the investigation of insulin signalling in a human cell culture model of adipogenesis. 胰岛素信号在脂肪生成的人类细胞培养模型中研究的优化方案。

IF 3.3 4区生物学

Adipocyte Pub Date : 2023-12-01 DOI: 10.1080/21623945.2023.2179339

Jonathan M Gamwell, Keanu Paphiti, Leanne Hodson, Fredrik Karpe, Katherine E Pinnick, Marijana Todorčević

{"title":"An optimised protocol for the investigation of insulin signalling in a human cell culture model of adipogenesis.","authors":"Jonathan M Gamwell, Keanu Paphiti, Leanne Hodson, Fredrik Karpe, Katherine E Pinnick, Marijana Todorčević","doi":"10.1080/21623945.2023.2179339","DOIUrl":"10.1080/21623945.2023.2179339","url":null,"abstract":"While there is no standardized protocol for the differentiation of human adipocytes in culture, common themes exist in the use of supra-physiological glucose and hormone concentrations, and an absence of exogenous fatty acids. These factors can have detrimental effects on some aspects of adipogenesis and adipocyte function. Here, we present methods for modifying the adipogenic differentiation protocol to overcome impaired glucose uptake and insulin signalling in human adipose-derived stem cell lines derived from the stromal vascular fraction of abdominal and gluteal subcutaneous adipose tissue. By reducing the length of exposure to adipogenic hormones, in combination with a physiological glucose concentration (5 mM), and the provision of exogenous fatty acids (reflecting typical dietary fatty acids), we were able to restore early insulin signalling events and glucose uptake, which were impaired by extended use of hormones and a high glucose concentration, respectively. Furthermore, the addition of exogenous fatty acids greatly increased the storage of triglycerides and removed the artificial demand to synthesize all fatty acids by de novo lipogenesis. Thus, modifying the adipogenic cocktail can enhance functional aspects of human adipocytes in vitro and is an important variable to consider prior to in vitro investigations into adipocyte biology.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"12 1","pages":"2179339"},"PeriodicalIF":3.3,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9980465/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9463287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An optimized method for Oil Red O staining with the salicylic acid ethanol solution. 水杨酸乙醇溶液对油红O染色的优化方法。

IF 3.5 4区生物学

Adipocyte Pub Date : 2023-12-01 DOI: 10.1080/21623945.2023.2179334

Junbao Du, Li Zhao, Quan Kang, Yun He, Yang Bi

引用次数: 0

Adipose-derived mesenchymal stem cell-secreted extracellular vesicles alleviate non-alcoholic fatty liver disease via delivering miR-223-3p. 脂肪源性间充质干细胞分泌的细胞外囊泡通过传递miR-223-3p缓解非酒精性脂肪肝疾病。

IF 3.3 4区生物学

Adipocyte Pub Date : 2022-12-01 DOI: 10.1080/21623945.2022.2098583

Qinghui Niu, Ting Wang, Zhiqiang Wang, Feng Wang, Deyu Huang, Huali Sun, Hanyun Liu

{"title":"Adipose-derived mesenchymal stem cell-secreted extracellular vesicles alleviate non-alcoholic fatty liver disease via delivering miR-223-3p.","authors":"Qinghui Niu, Ting Wang, Zhiqiang Wang, Feng Wang, Deyu Huang, Huali Sun, Hanyun Liu","doi":"10.1080/21623945.2022.2098583","DOIUrl":"https://doi.org/10.1080/21623945.2022.2098583","url":null,"abstract":"Increasing studies have identified the potential of mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) in non-alcoholic fatty liver disease (NAFLD) treatment. Hence, we further focused on the potential of adipose-derived MSC (ADSC)-EVs in NAFLD by delivering miR-223-3p. The uptake of isolated ADSC-EVs by hepatocytes was assessed, and the expression of miR-223-3p in ADSC-EVs and hepatocytes was characterized. It was established that miR-223-3p, enriched in ADSC-EVs, could be delivered by ADSC-EVs into hepatocytes. Using co-culture system and gain-of-function approach, we evaluated the effect of ADSC-EVs carrying miR-223-3p on lipid accumulation and liver fibrosis in pyrrolizidine alkaloids (PA)-induced hepatocytes and a high-fat diet-induced NAFLD mouse model. Bioinformatics websites and dual-luciferase reporter gene assay were performed to determine the interactions between miR-223-3p and E2F1, which was further validated by rescue experiments. ADSC-EVs containing miR-223-3p displayed suppressive effects on lipid accumulation and liver fibrosis through E2F1 inhibition, since E2F1 was demonstrated as a target gene of miR-223-3p. The protective role of ADSC-EVs by delivering miR-223-3p was then confirmed in the mouse model. Collectively, this study elucidated that ADSC-EVs delayed the progression NAFLD through the delivery of anti-fibrotic miR-223-3p and subsequent E2F1 suppression, which may suggest miR-223-3p-loaded ADSC-EVs to be a potential therapeutic approach for NAFLD.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"11 1","pages":"572-587"},"PeriodicalIF":3.3,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9481107/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10626499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Cixutumumab reveals a critical role for IGF-1 in adipose and hepatic tissue remodelling during the development of diet-induced obesity. 环妥珠单抗揭示了IGF-1在饮食性肥胖发展过程中脂肪和肝组织重塑中的关键作用。

IF 3.3 4区生物学

Adipocyte Pub Date : 2022-12-01 DOI: 10.1080/21623945.2022.2089394

Helen Imrie, Hema Viswambharan, Natalie J Haywood, Katherine I Bridge, Nadira Y Yuldasheva, Stacey Galloway, Katie J Simmons, Richard M Cubbon, Piruthivi Sukumar, Nicole T Watt, Laeticia Lichtenstein, Judy I Wyatt, Hiromi Kudo, Robert Goldin, Baptiste Rode, Stephen B Wheatcroft, Mark T Kearney

{"title":"Cixutumumab reveals a critical role for IGF-1 in adipose and hepatic tissue remodelling during the development of diet-induced obesity.","authors":"Helen Imrie, Hema Viswambharan, Natalie J Haywood, Katherine I Bridge, Nadira Y Yuldasheva, Stacey Galloway, Katie J Simmons, Richard M Cubbon, Piruthivi Sukumar, Nicole T Watt, Laeticia Lichtenstein, Judy I Wyatt, Hiromi Kudo, Robert Goldin, Baptiste Rode, Stephen B Wheatcroft, Mark T Kearney","doi":"10.1080/21623945.2022.2089394","DOIUrl":"https://doi.org/10.1080/21623945.2022.2089394","url":null,"abstract":"High fat diet (HFD)-induced obesity leads to perturbation in the storage function of white adipose tissue (WAT) resulting in deposition of lipids in tissues ill-equipped to deal with this challenge. The role of insulin like growth factor-1 (IGF-1) in the systemic and organ-specific responses to HFD is unclear. Using cixutumumab, a monoclonal antibody that internalizes and degrades cell surface IGF-1 receptors (IGF-1 R), leaving insulin receptor expression unchanged we aimed to establish the role of IGF-1 R in the response to a HFD. Mice treated with cixutumumab fed standard chow developed mild hyperinsulinemia with no change in WAT. When challenged by HFD mice treated with cixutumumab had reduced weight gain, reduced WAT expansion, and reduced hepatic lipid vacuole formation. In HFD-fed mice, cixutumumab led to reduced levels of genes encoding proteins important in fatty acid metabolism in WAT and liver. Cixutumumab protected against blunting of insulin-stimulated phosphorylation of Akt in liver of HFD fed mice. These data reveal an important role for IGF-1 R in the WAT and hepatic response to short-term nutrient excess. IGF-1 R inhibition during HFD leads to a lipodystrophic phenotype with a failure of WAT lipid storage and protection from HFD-induced hepatic insulin resistance.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"11 1","pages":"366-378"},"PeriodicalIF":3.3,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9235901/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9114661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Dipeptidyl peptidase-4 cell surface expression marks an abundant adipose stem/progenitor cell population with high stemness in human white adipose tissue. 二肽基肽酶-4细胞表面表达标志着人类白色脂肪组织中存在丰富的高干性脂肪干/祖细胞群。

IF 3.3 4区生物学

Adipocyte Pub Date : 2022-12-01 DOI: 10.1080/21623945.2022.2129060

Florian M Hatzmann, Sonja Großmann, Petra Waldegger, G Jan Wiegers, Markus Mandl, Tina Rauchenwald, Gerhard Pierer, Werner Zwerschke

{"title":"Dipeptidyl peptidase-4 cell surface expression marks an abundant adipose stem/progenitor cell population with high stemness in human white adipose tissue.","authors":"Florian M Hatzmann, Sonja Großmann, Petra Waldegger, G Jan Wiegers, Markus Mandl, Tina Rauchenwald, Gerhard Pierer, Werner Zwerschke","doi":"10.1080/21623945.2022.2129060","DOIUrl":"https://doi.org/10.1080/21623945.2022.2129060","url":null,"abstract":"The capacity of adipose stem/progenitor cells (ASCs) to undergo self-renewal and differentiation is crucial for adipose tissue homoeostasis, regeneration and expansion. However, the heterogeneous ASC populations of the adipose lineage constituting adipose tissue are not precisely known. In the present study, we demonstrate that cell surface expression of dipeptidyl peptidase-4 (DPP4)/cluster of differentiation 26 (CD26) subdivides the DLK1-/CD34+/CD45-/CD31- ASC pool of human white adipose tissues (WATs) into two large populations. Ex vivo, DPP4+ ASCs possess higher self-renewal and proliferation capacity and lesser adipocyte differentiation potential than DDP4- ASCs. The knock-down of DPP4 in ASC leads to significantly reduced proliferation and self-renewal capacity, while adipogenic differentiation is increased. Ectopic overexpression of DPP4 strongly inhibits adipogenesis. Moreover, in whole mount stainings of human subcutaneous (s)WAT, we detect DPP4 in CD34+ ASC located in the vascular stroma surrounding small blood vessels and in mature adipocytes. We conclude that DPP4 is a functional marker for an abundant ASC population in human WAT with high proliferation and self-renewal potential and low adipogenic differentiation capacity.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"11 1","pages":"601-615"},"PeriodicalIF":3.3,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9542856/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9180569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Comparative transcriptomic analysis of rabbit interscapular brown adipose tissue whitening under physiological conditions. 生理条件下兔肩胛间褐色脂肪组织变白的比较转录组学分析。

IF 3.3 4区生物学

Adipocyte Pub Date : 2022-12-01 DOI: 10.1080/21623945.2022.2111053

Lei Li, Qian Wan, Qiaoyun Long, Tao Nie, Shiting Zhao, Liufeng Mao, Chuanli Cheng, Chao Zou, Kerry Loomes, Aimin Xu, Liangxue Lai, Xin Liu, Ziyuan Duan, Xiaoyan Hui, Donghai Wu

{"title":"Comparative transcriptomic analysis of rabbit interscapular brown adipose tissue whitening under physiological conditions.","authors":"Lei Li, Qian Wan, Qiaoyun Long, Tao Nie, Shiting Zhao, Liufeng Mao, Chuanli Cheng, Chao Zou, Kerry Loomes, Aimin Xu, Liangxue Lai, Xin Liu, Ziyuan Duan, Xiaoyan Hui, Donghai Wu","doi":"10.1080/21623945.2022.2111053","DOIUrl":"https://doi.org/10.1080/21623945.2022.2111053","url":null,"abstract":"Interscapular brown adipose tissue (iBAT) of both rabbits and humans exhibits a similar whitening phenomenon under physiological conditions. However, a detailed characterization of iBAT whitening in them is still lacking. Here, we chose rabbits as a model to gain a better understanding of the molecular signature changes during the whitening process of iBAT by transcriptomic analysis of rabbit iBAT at day 1, day 14, 1 month and 4 months after birth. We applied non-invasive MRI imaging to monitor the whitening process and correlated these changes with analysis of morphological, histological and molecular features. Principal component analysis (PCA) of differentially expressed genes delineated three major phases for the whitening process as Brown, Transition and Whitened BAT phases. RNA-sequencing data revealed that whitening of iBAT was an orchestrated process where multiple types of cells and tissues participated in a variety of physiological processes including neovascularization, formation of new nervous networks and immune regulation. Several key metabolic and signalling pathways contributed to whitening of iBAT, and immune cells and immune regulation appeared to play an overarching role.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"11 1","pages":"529-549"},"PeriodicalIF":3.3,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9427046/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10625211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Salvianolic acid A promotes mitochondrial biogenesis and mitochondrial function in 3T3-L1 adipocytes through regulation of the AMPK-PGC1α signalling pathway. 丹酚酸A通过调节AMPK-PGC1α信号通路促进3T3-L1脂肪细胞的线粒体生物发生和线粒体功能。

IF 3.3 4区生物学

Adipocyte Pub Date : 2022-12-01 DOI: 10.1080/21623945.2022.2116790

Jialin Sun, Ping Leng, Xiao Li, Qie Guo, Jun Zhao, Yu Liang, Xiaolei Zhang, Xue Yang, Jing Li

{"title":"Salvianolic acid A promotes mitochondrial biogenesis and mitochondrial function in 3T3-L1 adipocytes through regulation of the AMPK-PGC1α signalling pathway.","authors":"Jialin Sun, Ping Leng, Xiao Li, Qie Guo, Jun Zhao, Yu Liang, Xiaolei Zhang, Xue Yang, Jing Li","doi":"10.1080/21623945.2022.2116790","DOIUrl":"https://doi.org/10.1080/21623945.2022.2116790","url":null,"abstract":"Mitochondrial dysfunction is associated with insulin resistance and type 2 diabetes (T2DM). Decreased mitochondrial abundance and function were found in white adipose tissue (WAT) of T2DM patients. Therefore, promoting WAT mitochondrial biogenesis and improving adipocyte metabolism may be strategies to prevent and reverse T2DM. Salvianolic acid A (SAA) has been found to exert anti-diabetic and lipid disorder-improving effects. However whether SAA benefits mitochondrial biogenesis and function in adipose tissue is unclear. Here, we evaluated SAA's effect on mitochondrial biogenesis and function in 3T3-L1 adipocytes and investigated its potential regulatory mechanism. Results showed that SAA treatment significantly promoted the transcription and expression of peroxisome proliferator-activated receptor γ coactivator- 1α (PGC-1α), nuclear respiratory factor 1 (NRF1) and mitochondrial transcription factor A (TFAM). Meanwhile, SAA treatment significantly promoted mitochondrial biogenesis by increasing mitochondrial DNA (mtDNA) quantity, mitochondrial mass, and expression of mitochondrial respiratory chain enzyme complexes III and complex IV. These enhancements were accompanied by enhanced phosphorylation of AMPK and ACC and were suppressed by Compound C, a specific AMPK inhibitor. Furthermore, SAA treatment improved adipocytes mitochondrial respiration and stimulated ATP generation. These findings indicate that SAA exerts a potential therapeutic capacity against adipocytes mitochondrial dysfunction in diabetes by activating the AMPK-PGC-1α pathway.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"11 1","pages":"562-571"},"PeriodicalIF":3.3,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9450893/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10625214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Bioinformatics analysis to obtain critical genes regulated in subcutaneous adipose tissue after bariatric surgery. 生物信息学分析以获得减肥手术后皮下脂肪组织中调节的关键基因。

IF 3.3 4区生物学

Adipocyte Pub Date : 2022-12-01 DOI: 10.1080/21623945.2022.2115212

Shuai Chen, Yicheng Jiang, Xiaoyang Qi, Peng Song, Liming Tang, Hanyang Liu

{"title":"Bioinformatics analysis to obtain critical genes regulated in subcutaneous adipose tissue after bariatric surgery.","authors":"Shuai Chen, Yicheng Jiang, Xiaoyang Qi, Peng Song, Liming Tang, Hanyang Liu","doi":"10.1080/21623945.2022.2115212","DOIUrl":"https://doi.org/10.1080/21623945.2022.2115212","url":null,"abstract":"Bariatric surgery (BS) is a dependable method for managing obesity and metabolic diseases, however, the regulatory processes of lipid metabolism are still not well elucidated. Differentially expressed genes (DEGs) were analysed through three transcriptomic datasets of GSE29409, GSE59034 and GSE72158 from the GEO database regarding subcutaneous adipose tissue (SAT) after BS, and 37 DEGs were identified. The weighted gene co-expression network analysis (WGCNA), last absolute shrinkage and selection operator (LASSO) logistic regression and support vector machine-recursive feature elimination (SVM-RFE) algorithms further screened four key genes involved in the regulation of STMN2, SFRP4, APOE and MXRA5. The GSE53376 dataset was used to further confirm the differential expression of SFRP4, APOE and MXRA5 in the postoperative period. GSEA analysis reveals activation of immune-related regulatory pathways after surgery. Finally, the silencing of MXRA5 was found by experimental methods to affect the expression of PPARγ and CEBPα during the differentiation of preadipocytes, as well as to affect the formation of lipid droplets. In conclusion, SAT immunoregulation was mobilized after BS, while MXRA5 was involved in the regulation of lipid metabolism.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"11 1","pages":"550-561"},"PeriodicalIF":3.3,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9427031/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10623124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Identification and characterization of novel abdominal and pelvic brown adipose depots in mice. 小鼠腹部和骨盆新型棕色脂肪库的鉴定和表征。

IF 3.3 4区生物学

Adipocyte Pub Date : 2022-12-01 DOI: 10.1080/21623945.2022.2133415

Ana M Mesa, Theresa I Medrano, Vijay K Sirohi, William H Walker, Richard D Johnson, Sergei G Tevosian, Angie M Adkin, Paul S Cooke

{"title":"Identification and characterization of novel abdominal and pelvic brown adipose depots in mice.","authors":"Ana M Mesa, Theresa I Medrano, Vijay K Sirohi, William H Walker, Richard D Johnson, Sergei G Tevosian, Angie M Adkin, Paul S Cooke","doi":"10.1080/21623945.2022.2133415","DOIUrl":"https://doi.org/10.1080/21623945.2022.2133415","url":null,"abstract":"Brown adipose tissue (BAT) generates heat through non-shivering thermogenesis, and increasing BAT amounts or activity could facilitate obesity treatment and provide metabolic benefits. In mice, BAT has been reported in perirenal, thoracic and cranial sites. Here, we describe new pelvic and lower abdominal BAT depots located around the urethra, internal reproductive and urinary tract organs and major lower pelvic blood vessels, as well as between adjacent muscles where the upper hind leg meets the abdominal cavity. Immunohistochemical, western blot and PCR analyses revealed that these tissues expressed BAT markers such as uncoupling protein 1 (UCP1) and CIDEA, but not white adipose markers, and β3-adrenergic stimulation increased UCP1 amounts, a classic characteristic of BAT tissue. The newly identified BAT stores contained extensive sympathetic innervation with high mitochondrial density and multilocular lipid droplets similar to interscapular BAT. BAT repositories were present and functional neonatally, and showed developmental changes between the neonatal and adult periods. In summary, several new depots showing classical BAT characteristics are reported and characterized in the lower abdominal/pelvic region of mice. These BAT stores are likely significant metabolic regulators in the mouse and some data suggests that similar BAT depots may also exist in humans.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"11 1","pages":"616-629"},"PeriodicalIF":3.3,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9586652/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10625700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adipocyte最新文献