Adipocyte最新文献_第10页

Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) influences adipocytes injuries through triggering XBP1 and activating mitochondria-mediated apoptosis. 牙龈卟啉单胞菌脂多糖(Pg-LPS)通过触发XBP1和激活线粒体介导的细胞凋亡影响脂肪细胞损伤。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2020.1856527

Ying-Tao Lv, Jin-Jin Zeng, Jia-Yi Lu, Xue-Yang Zhang, Ping-Ping Xu, Yuan Su

{"title":"Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) influences adipocytes injuries through triggering XBP1 and activating mitochondria-mediated apoptosis.","authors":"Ying-Tao Lv, Jin-Jin Zeng, Jia-Yi Lu, Xue-Yang Zhang, Ping-Ping Xu, Yuan Su","doi":"10.1080/21623945.2020.1856527","DOIUrl":"https://doi.org/10.1080/21623945.2020.1856527","url":null,"abstract":"Obesity is an important public-health problem worldwide. This study aimed to determine effects of porphyromonas gingivalis lipopolysaccharide (Pg-LPS) on adipocytes injuries and explore associated mechanisms. Adipocytes were isolated from SD rats. pLVX-XBP1 (XBP1 over-expression) and pLVX-XBP1-RNAi (silencing XBP1) were structured and transfected into adipocytes. All adipocytes were divided into pLVX-NC, pLVX-XBP1, pLVX-NC+Pg-LPS and pLVX-XBP1+ Pg-LPS group. Oil-Red O staining was employed to identify isolated adipocytes. Quantitative real-time PCR (qRT-PCR) was used to examine gene transcription of IL-6, TNF-α, leptin, adiponectin. Western blotting was used to detect Bax and caspase-3 expression. Adipocytes were successfully isolated and identified with Oil-Red O staining. Both XBP1 mimic and XBP1 RNAi were effectively transfected into adipocytes with higher expressing efficacy. XBP1 over-expression significantly aggravated Pg-LPS induced inflammatory response compared to adipocytes without Pg-LPS treatment (p<0.05). Pg-LPS significantly enhanced leptin and inhibited adiponectin expression by up-regulating XBP1 expression (p<0.05). XBP1 silence significantly alleviated Pg-LPS induced inflammatory response and reduced leptin, enhanced adiponectin expression in Pg-LPS treated adipocytes compared to adipocytes without Pg-LPS treatment (p<0.05). Pg-LPS induced apoptosis of adipocytes by enhancing XBP1 expression and modulating Bcl-2/Bax pathway associated molecules. In conclusion, Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) induces adipocytes injuries through modulating XBP1 expression and initialling mitochondria-mediated apoptosis.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21623945.2020.1856527","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39125480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

The characterization of metabolites alterations in white adipose tissue of diabetic GK Rats after ileal transposition surgery by an untargeted metabolomics approach. 非靶向代谢组学方法表征糖尿病GK大鼠回肠转位手术后白色脂肪组织中代谢物的变化。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1926139

Xiaorui Lyu, Kemin Yan, Weijie Chen, Yujie Wang, Huijuan Zhu, Hui Pan, Guole Lin, Linjie Wang, Hongbo Yang, Fengying Gong

{"title":"The characterization of metabolites alterations in white adipose tissue of diabetic GK Rats after ileal transposition surgery by an untargeted metabolomics approach.","authors":"Xiaorui Lyu, Kemin Yan, Weijie Chen, Yujie Wang, Huijuan Zhu, Hui Pan, Guole Lin, Linjie Wang, Hongbo Yang, Fengying Gong","doi":"10.1080/21623945.2021.1926139","DOIUrl":"https://doi.org/10.1080/21623945.2021.1926139","url":null,"abstract":"Dysfunction of adipose tissue could lead to insulin resistance, obesity and type 2 diabetes. Thus, our present study aimed to investigate metabolites alterations in white adipose tissue (WAT) of diabetic GK rats after IT surgery. Ten-week-old male diabetic GK rats were randomly subjected to IT and Sham-IT surgery. Six weeks later, the untargeted metabolomics in WAT of diabetic GK rats was performed. Differential metabolites were selected according to the coefficient of variation (CV) of quality control (QC) sample <30%, variable importance in the projection (VIP) >1 and P < 0.05. Then, the hierarchical clustering of differential metabolites was conducted and the KEGG database was used for metabolic pathway analysis. A total of 50 (in positive ion mode) and 68 (in negative ion mode) metabolites were identified as differential metabolites in WAT of diabetic GK rats between IT group and Sham-IT group, respectively. These differential metabolites were well clustered, which in descending order of the number of involved differential metabolites is ubiquinone and other terpenoid-quinone biosynthesis, AMPK signalling pathway, pantothenate and CoA biosynthesis, ferroptosis, vitamin digestion and absorption, glycerophospholipid metabolism, phenylalanine metabolism, steroid hormone biosynthesis, neuroactive ligand-receptor interaction, porphyrin and chlorophyll metabolism and bile secretion, and correlated with the parameters of body weight, food intake, WAT mass and glucose metabolism, which were significantly improved after IT surgery. The differential metabolites in WAT of diabetic GK rats were mainly related to the pathway of energy metabolism, and correlated with the improved phenotypes of diabetic GK rats after IT surgery.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21623945.2021.1926139","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38889495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Reliability and variation in mitochondrial respiration in human adipose tissue. 人类脂肪组织中线粒体呼吸的可靠性和变异。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1991617

Ronni Eg Sahl, Eva Frederikke Høy Helms, Malte Schmücker, Mathias Flensted-Jensen, Arthur Ingersen, Thomas Morville, Flemming Dela, Jørn Wulff Helge, Steen Larsen

引用次数: 2

Association between rs174537 FADS1 polymorphism and immune cell profiles in abdominal and femoral subcutaneous adipose tissue: an exploratory study in adults with obesity. rs174537 FADS1多态性与腹部和股皮下脂肪组织免疫细胞谱之间的关系:一项针对肥胖成人的探索性研究

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1888470

Chenxuan Wang, Jessica Murphy, Kerri Z Delaney, Natalie Khor, José A Morais, Michael A Tsoukas, Dana E Lowry, David M Mutch, Sylvia Santosa

{"title":"Association between rs174537 FADS1 polymorphism and immune cell profiles in abdominal and femoral subcutaneous adipose tissue: an exploratory study in adults with obesity.","authors":"Chenxuan Wang, Jessica Murphy, Kerri Z Delaney, Natalie Khor, José A Morais, Michael A Tsoukas, Dana E Lowry, David M Mutch, Sylvia Santosa","doi":"10.1080/21623945.2021.1888470","DOIUrl":"https://doi.org/10.1080/21623945.2021.1888470","url":null,"abstract":"Fatty acid desaturase 1 (FADS1) polymorphisms alter fatty acid content in subcutaneous adipose tissue (SAT); however, existing evidence is limited and conflicting regarding the association between FADS1 variants and SAT inflammatory status. To advance this area, we conducted an exploratory study to investigate whether the common rs174537 polymorphism in FADS1 was associated with immune cell profiles in abdominal and femoral SAT in individuals with obesity. FADS1 gene expression and immune cell profiles in SAT depots were assessed by qPCR and flow cytometry, respectively. Although FADS1 gene expression was associated with genotype, no associations were observed with immune cell profiles in either depot. Our study provides additional evidence that rs174537 in FADS1 has minimal impact on inflammatory status in obese SAT.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21623945.2021.1888470","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25380108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Low glucose and serum levels cause an increased inflammatory factor in 3T3-L1 cell through Akt, MAPKs and NF-кB activation. 低血糖和低血清水平通过激活Akt、MAPKs和NF-кB导致3T3-L1细胞炎症因子升高。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1914420

Hirona Kugo, Wanida Sukketsiri, Kazuko Iwamoto, Satoki Suihara, Tatsuya Moriyama, Nobuhiro Zaima

{"title":"Low glucose and serum levels cause an increased inflammatory factor in 3T3-L1 cell through Akt, MAPKs and NF-кB activation.","authors":"Hirona Kugo, Wanida Sukketsiri, Kazuko Iwamoto, Satoki Suihara, Tatsuya Moriyama, Nobuhiro Zaima","doi":"10.1080/21623945.2021.1914420","DOIUrl":"https://doi.org/10.1080/21623945.2021.1914420","url":null,"abstract":"Abdominal aortic aneurysm (AAA) involves the degradation of vascular fibres, and dilation and rupture of the abdominal aorta. Hypoperfusion in the vascular walls due to stenosis of the vasa vasorum is reportedly a cause of AAA onset and involves the induction of adventitial ectopic adipocytes. Recent studies have reported that ectopic adipocytes are associated with AAA rupture in both human and hypoperfusion-induced animal models, highlighting the pathological importance of hypoperfusion and adipocytes in AAA. However, the relationship between hypoperfusion and AAA remains unknown. In this study, we investigated the changes in inflammation-related factors in adipocytes at low glucose and serum levels. Low glucose and serum levels enhanced the production of AAA-related factors in 3T3-L1 cells. Low glucose and serum levels increased the activation of protein kinase B (also known as Akt), extracellular signal-regulated protein kinase 1/2, p38, c-Jun N-terminal kinase, and nuclear factor (NF) кB at the protein level. The inflammatory factors and related signalling pathways were markedly decreased following the return of the cells to normal culture conditions. These data suggest that low glucose and serum levels increase the levels of inflammatory factors through the activation of Akt, mitogen activated protein kinase, and NF-κB signalling pathways.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21623945.2021.1914420","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38826833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Staufen1 unwinds the secondary structure and facilitates the translation of fatty acid binding protein 4 mRNA during adipogenesis. 在脂肪形成过程中，Staufen1解开二级结构，促进脂肪酸结合蛋白4mrna的翻译。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1948165

Xiaodi Liang, Yi Jiao, Xueli Gong, Hao Gu, Nuerbiye Nuermaimaiti, Xuanyu Meng, Dihui Liu, Yaqun Guan

{"title":"Staufen1 unwinds the secondary structure and facilitates the translation of fatty acid binding protein 4 mRNA during adipogenesis.","authors":"Xiaodi Liang, Yi Jiao, Xueli Gong, Hao Gu, Nuerbiye Nuermaimaiti, Xuanyu Meng, Dihui Liu, Yaqun Guan","doi":"10.1080/21623945.2021.1948165","DOIUrl":"https://doi.org/10.1080/21623945.2021.1948165","url":null,"abstract":"Adipogenesis is regulated by genetic interactions, in which post-transcriptional regulation plays an important role. Staufen double-stranded RNA binding protein 1 (Staufen1 or STAU1) plays diverse roles in RNA processing and adipogenesis. Previously, we found that the downregulation of STAU1 affects the expression of fatty acid-binding protein 4 (FABP4) at the protein level but not at the mRNA level. This study aimed to determine the mechanism underlying the regulation of FABP4 expression by STAU1, explaining the inconsistency between FABP4 mRNA and protein levels. We used RNA interference, photoactivatable ribonucleoside enhanced cross-linking and immunoprecipitation, and an adeno-associated virus to examine the functions of STAU1 in adipogenesis. Our results indicate that STAU1 binds to the coding sequences of FABP4, thereby regulating the translation of FABP4 mRNA by unwinding the double-stranded structure. Furthermore, STAU1 mediates adipogenesis by regulating the secretion of free fatty acids. However, STAU1 knockdown decreases the fat weight/body weight ratio but does not affect the plasma triglyceride levels. These findings describe the mechanisms involved in STAU1-mediated regulation of FABP4 expression at the translational level during adipogenesis.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21623945.2021.1948165","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39151741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Hematopoietic stem cells produce intermediate lineage adipocyte progenitors that simultaneously express both myeloid and mesenchymal lineage markers in adipose tissue. 造血干细胞产生中间谱系脂肪祖细胞，同时表达脂肪组织中的髓系和间充质谱系标记。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1957290

Kathleen M Gavin, Timothy M Sullivan, Joanne K Maltzahn, Jeremy T Rahkola, Alistair S Acosta, Wendy M Kohrt, Susan M Majka, Dwight J Klemm

{"title":"Hematopoietic stem cells produce intermediate lineage adipocyte progenitors that simultaneously express both myeloid and mesenchymal lineage markers in adipose tissue.","authors":"Kathleen M Gavin, Timothy M Sullivan, Joanne K Maltzahn, Jeremy T Rahkola, Alistair S Acosta, Wendy M Kohrt, Susan M Majka, Dwight J Klemm","doi":"10.1080/21623945.2021.1957290","DOIUrl":"https://doi.org/10.1080/21623945.2021.1957290","url":null,"abstract":"Some adipocytes are produced from bone marrow hematopoietic stem cells. In vitro studies previously indicated that these bone marrow-derived adipocytes (BMDAs) were generated from adipose tissue macrophage (ATM) that lose their hematopoietic markers and acquire mesenchymal markers prior to terminal adipogenic differentiation. Here we interrogated whether this hematopoietic-to-mesenchymal transition drives BMDA production In vitro. We generated transgenic mice in which the lysozyme gene promoter (LysM) indelibly labeled ATM with green fluorescent protein (GFP). We discovered that adipose stroma contained a population of LysM-positive myeloid cells that simultaneously expressed hematopoietic/myeloid markers (CD45 and CD11b), and mesenchymal markers (CD29, PDGFRa and Sca-1) typically found on conventional adipocyte progenitors. These cells were capable of adipogenic differentiation In vitro and In vitro, while other stromal populations deficient in PDGFRa and Sca-1 were non-adipogenic. BMDAs and conventional adipocytes expressed common fat cell markers but exhibited little or no expression of hematopoietic and mesenchymal progenitor cell markers. The data indicate that BMDAs are produced from ATM simultaneously expressing hematopoietic and mesenchymal markers rather than via a stepwise hematopoietic-to-mesenchymal transition. Because BMDA production is stimulated by high fat feeding, their production from hematopoietic progenitors may maintain adipocyte production when conventional adipocyte precursors are diminished.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8381847/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39320618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Application of a deep learning-based image analysis and live-cell imaging system for quantifying adipogenic differentiation kinetics of adipose-derived stem/stromal cells. 应用基于深度学习的图像分析和活细胞成像系统定量脂肪源性干细胞/基质细胞的成脂分化动力学。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.2000696

Patrick Terrence Brooks, Lea Munthe-Fog, Klaus Rieneck, Frederik Banch Clausen, Olga Ballesteros Rivera, Eva Kannik Haastrup, Anne Fischer-Nielsen, Jesper Dyrendom Svalgaard

{"title":"Application of a deep learning-based image analysis and live-cell imaging system for quantifying adipogenic differentiation kinetics of adipose-derived stem/stromal cells.","authors":"Patrick Terrence Brooks, Lea Munthe-Fog, Klaus Rieneck, Frederik Banch Clausen, Olga Ballesteros Rivera, Eva Kannik Haastrup, Anne Fischer-Nielsen, Jesper Dyrendom Svalgaard","doi":"10.1080/21623945.2021.2000696","DOIUrl":"https://doi.org/10.1080/21623945.2021.2000696","url":null,"abstract":"ABSTRACT Quantitative methods for assessing differentiative potency of adipose-derived stem/stromal cells may lead to improved clinical application of this multipotent stem cell, by advancing our understanding of specific processes such as adipogenic differentiation. Conventional cell staining methods are used to determine the formation of adipose areas during adipogenesis as a qualitative representation of adipogenic potency. Staining methods such as oil-red-O are quantifiable using absorbance measurements, but these assays are time and material consuming. Detection methods for cell characteristics using advanced image analysis by machine learning are emerging. Here, live-cell imaging was combined with a deep learning-based detection tool to quantify the presence of adipose areas and lipid droplet formation during adipogenic differentiation of adipose-derived stem/stromal cells. Different detection masks quantified adipose area and lipid droplet formation at different time points indicating kinetics of adipogenesis and showed differences between individual donors. Whereas CEBPA and PPARG expression seems to precede the increase in adipose area and lipid droplets, it might be able to predict expression of ADIPOQ. The applied method is a proof of concept, demonstrating that deep learning methods can be used to investigate adipogenic differentiation and kinetics in vitro using specific detection masks based on algorithm produced from annotation of image data.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8632106/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39705456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Verapamil induces autophagy to improve liver regeneration in non-alcoholic fatty liver mice. 维拉帕米诱导自噬促进非酒精性脂肪肝小鼠肝脏再生。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1983241

Jian-Lin Lai, Yuan-E Lian, Jun-Yi Wu, Yao-Dong Wang, Yan-Nan Bai

{"title":"Verapamil induces autophagy to improve liver regeneration in non-alcoholic fatty liver mice.","authors":"Jian-Lin Lai, Yuan-E Lian, Jun-Yi Wu, Yao-Dong Wang, Yan-Nan Bai","doi":"10.1080/21623945.2021.1983241","DOIUrl":"10.1080/21623945.2021.1983241","url":null,"abstract":"Verapamil can restore intracellular calcium homeostasis, increase the fusion of autophagosomes and lysosomes, reduce lipid droplet accumulation and inhibit inflammation and insulin resistance in high-fat-fed mice. The present study aimed to investigate verapamil's effect and its underlying liver regeneration mechanism in mice with non-alcoholic fatty liver. After 50% hepatectomy was performed, the changes of autophagy and liver regeneration were evaluated by detecting cell proliferation and autophagy at each time point. Then, 25mg/kg verapamil was injected intraperitoneally for 10 d before an operation in the mild to moderate fatty liver and severe fatty liver groups. The control group and mild to moderate fatty liver group reached the peak of proliferation at 24-48h after operation, and the mice with severe fatty liver and steatohepatitis reached the peak at 48-72h. Autophagy in the normal group and mild to moderate fatty liver group reached the peak 48 hours after operation. Verapamil injection can enhance autophagy, reduce the weight of fatty liver mice, improve liver function and liver regeneration. Verapamil can induce autophagy, improve hepatocyte function and promote hepatocyte regeneration through the mTOR independent signaling pathway, thus improving the process of liver regeneration after partial hepatectomy.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8555529/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39558763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Composition, isolation, identification and function of adipose tissue-derived exosomes. 脂肪组织源性外泌体的组成、分离、鉴定和功能。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1983242

Rui Zhao, Tiantian Zhao, Zhaozhao He, Rui Cai, Weijun Pang

{"title":"Composition, isolation, identification and function of adipose tissue-derived exosomes.","authors":"Rui Zhao, Tiantian Zhao, Zhaozhao He, Rui Cai, Weijun Pang","doi":"10.1080/21623945.2021.1983242","DOIUrl":"https://doi.org/10.1080/21623945.2021.1983242","url":null,"abstract":"Exosomes are nano-sized extracellular vesicles (30-160 nm diameter) with lipid bilayer membrane secrete by various cells that mediate the communication between cells and tissue, which contain a variety of non-coding RNAs, mRNAs, proteins, lipids and other functional substances. Adipose tissue is important energy storage and endocrine organ in the organism. Recent studies have revealed that adipose tissue-derived exosomes (AT-Exosomes) play a critical role in many physiologically and pathologically functions. Physiologically, AT-Exosomes could regulate the metabolic homoeostasis of various organs or cells including liver and skeletal muscle. Pathologically, they could be used in the treatment of disease and or that they may be involved in the progression of the disease. In this review, we describe the basic principles and methods of exosomes isolation and identification, as well as further summary the specific methods. Moreover, we categorize the relevant studies of AT-Exosomes and summarize the different components and biological functions of mammalian exosomes. Most importantly, we elaborate AT-Exosomes crosstalk within adipose tissue and their functions on other tissues or organs from the physiological and pathological perspective. Based on the above analysis, we discuss what remains to be discovered problems in AT-Exosomes studies and prospect their directions needed to be further explored in the future.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8604391/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39570672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18