Adipocyte最新文献_第10页

MiR-33a plays an crucial role in the proliferation of bovine preadipocytes. MiR-33a 在牛前脂肪细胞的增殖过程中起着至关重要的作用。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1908655

Wenzhen Zhang, Li Wang, Sayed Haidar Abbas Raza, Xiaoyu Wang, Guohu Wang, Chengcheng Liang, Gong Cheng, Bingzhi Li, Linsen Zan

{"title":"MiR-33a plays an crucial role in the proliferation of bovine preadipocytes.","authors":"Wenzhen Zhang, Li Wang, Sayed Haidar Abbas Raza, Xiaoyu Wang, Guohu Wang, Chengcheng Liang, Gong Cheng, Bingzhi Li, Linsen Zan","doi":"10.1080/21623945.2021.1908655","DOIUrl":"10.1080/21623945.2021.1908655","url":null,"abstract":"Preadipocyte proliferation is a critical and precisely orchestrated procedure in adipogenesis, which is highly regulated by microRNAs (miRNAs). A previous study identified that the expression of miR-33a is different in intramuscular fat (IMF) tissues from steers and bulls. In the present study, miR-33a was overexpressed in bovine preadipocytes, and a total of 781 differentialy expressed genes were found, including 348 upregulated and 433 downregulated genes. Gene Ontology and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway analyses of the differentially expressed genes enriched cell division and cell cycle respectively. MiR-33a overexpression decreased the rate of preadipocyte proliferation. Synchronously, the mRNA and protein expression levels of proliferation-related marker genes, including cyclin B1 (CCNB1) and proliferating cell nuclear antigen (PCNA), were decreased. In contrast, inhibiting miR-33a increased the rate of preadipocyte proliferation, and expression levels of CCNB1 and PCNA. Furthermore, based on luciferase reporter assays, miR-33a targeted directly cyclin-dependent kinase 6 (CDK6)-3'UTR and inhibited CDK6 protein expression. Interestingly, the silencing of CDK6 inhibited bovine preadipocyte proliferation and proliferation-related genes. Therefore, miR-33a inhibits the proliferation of bovine preadipocytes. CDK6 is the target gene of miR-33a and may be involved in the effects of miR-33a on bovine preadipocyte proliferation.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"10 1","pages":"189-200"},"PeriodicalIF":3.3,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8043176/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25578325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serum amyloid A3 deficiency impairs in vitro and in vivo adipocyte differentiation. 血清淀粉样蛋白A3缺乏损害体外和体内脂肪细胞分化。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1916220

Ellen Vercalsteren, Christine Vranckx, Ines Vermeire, Max Gooijen, Roger Lijnen, Ilse Scroyen

{"title":"Serum amyloid A3 deficiency impairs in vitro and in vivo adipocyte differentiation.","authors":"Ellen Vercalsteren, Christine Vranckx, Ines Vermeire, Max Gooijen, Roger Lijnen, Ilse Scroyen","doi":"10.1080/21623945.2021.1916220","DOIUrl":"https://doi.org/10.1080/21623945.2021.1916220","url":null,"abstract":"ABSTRACT Obesity, caused by an excess adipose tissue, is one of the biggest health-threats of the 21st century. Adipose tissue expansion occurs through two processes: (i) hypertrophy, and (ii) hyperplasia, the formation of new adipocytes, also termed adipogenesis. Recently, serum amyloid A3 (Saa3) has been implicated in adipogenesis. Therefore, the aim of this study was to investigate the effect of Saa3 on adipogenesis using both an in vitro and in vivo murine model. Saa3 gene silenced pre-adipocytes ha a lower expression of pro-adipogenic markers and less lipid accumulation, indicating impaired adipogenesis. Furthermore, male NUDE mice, injected with Saa3 gene silenced pre-adipocytes developed smaller fat pads with smaller adipocytes and lower expression of pro-adipogenic markers than their control counterparts. This confirms that Saa3 gene silencing indeed impairs adipogenesis, both in vitro and in vivo. These results indicate a clear role for Saa3 in adipogenesis and open new perspectives in the battle against obesity.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"10 1","pages":"242-250"},"PeriodicalIF":3.3,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21623945.2021.1916220","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38913460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Extracellular vesicles derived from lipoaspirate fluid promote fat graft survival. 从吸脂液中提取的细胞外囊泡促进脂肪移植物存活。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1932355

Fangfei Nie, Pengbing Ding, Chen Zhang, Zhenmin Zhao, Hongsen Bi

{"title":"Extracellular vesicles derived from lipoaspirate fluid promote fat graft survival.","authors":"Fangfei Nie, Pengbing Ding, Chen Zhang, Zhenmin Zhao, Hongsen Bi","doi":"10.1080/21623945.2021.1932355","DOIUrl":"https://doi.org/10.1080/21623945.2021.1932355","url":null,"abstract":"Extracellular vesicles (EVs) are specific subcellular vesicles released by cells under various environmental conditions. Tumescent liposuction is a commonly used procedure in plastic surgery practice. In the present study, we aimed to extract EVs derived from lipoaspirate fluid (LF-EVs) and characterize them using transmission electron microscopy, nanoparticle tracking analysis, and western blotting. The global profiles of proteins and microRNAs from LF-EVs were identified, strongly suggesting a potential regulatory function of LF-EVs. In addition, we investigated the effects and mechanisms of LF-EVs on fat graft survival. Cell functional tests showed that LF-EVs promoted the proliferation, migration, and tube structure formation of human umbilical vein endothelial cells. LF-EVs also promoted the adipogenic differentiation of adipose tissue-derived stem cells. The results of animal experiments showed that the average weights of fat grafts in the LF-EVs-treated group were significantly higher than those in the control group. Histologically, there was less fibrosis, fewer cysts, and increased fat tissue survival in the LF-EVs group. Further investigations of angiogenic and adipogenic factors revealed that LF-EVs also promoted angiogenesis and exerted a pro-adipogenic effect in vivo. Our findings will help to elucidate the functions of LF-EVs and provide a reference dataset for future translational studies.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"10 1","pages":"293-309"},"PeriodicalIF":3.3,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21623945.2021.1932355","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39036626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Different effects of high-fat and high-sucrose diets on the physiology of perivascular adipose tissues of the thoracic and abdominal aorta. 高脂高糖日粮对胸腹主动脉血管周围脂肪组织生理机能的不同影响。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1965333

Tsukasa Sasoh, Hirona Kugo, Yuya Kondo, Kento Miyamoto, Momoka Minami, Mayo Higashihara, Hirokazu Kawamoto, Fumiaki Takeshita, Tatsuya Moriyama, Nobuhiro Zaima

{"title":"Different effects of high-fat and high-sucrose diets on the physiology of perivascular adipose tissues of the thoracic and abdominal aorta.","authors":"Tsukasa Sasoh, Hirona Kugo, Yuya Kondo, Kento Miyamoto, Momoka Minami, Mayo Higashihara, Hirokazu Kawamoto, Fumiaki Takeshita, Tatsuya Moriyama, Nobuhiro Zaima","doi":"10.1080/21623945.2021.1965333","DOIUrl":"https://doi.org/10.1080/21623945.2021.1965333","url":null,"abstract":"Vascular diseases such as atherosclerosis and aneurysms are associated with diet. Perivascular adipose tissue (PVAT) was reportedly involved in the regulation of vascular functions. It is suggested that imbalanced diets can cause PVAT inflammation and dysfunction as well as impaired vascular function. However, the association between diets and PVAT are not clearly understood. Here, we showed that a high-fat and a high-sucrose diet affected PVAT at different sites. A high-fat diet induced increased number of large-sized lipid droplets and increased CD (Cluster of differentiation) 68+ macrophage- and monocyte chemotactic protein (MCP)-1-positive areas in the abdominal aortic PVAT (aPVAT). In addition, a high-fat diet caused decreased collagen fibre-positive area and increased CD68+ macrophage- and MCP-1-positive areas in the abdominal aorta. In contrast, a high-sucrose diet induced increased number of large-sized lipid droplets, increased CD68+ macrophage- and MCP-1-positive areas, and decreased UCP-1 positive area in the thoracic aortic PVAT (tPVAT). A high-sucrose diet caused decreased collagen fibre-positive area and increased CD68+ macrophage- and MCP-1-positive areas in the thoracic aorta. These results could be attributed to the different adipocyte populations in the tPVAT and aPVAT. Our results provide pathological evidence to improve our understanding of the relationship between diet and vascular diseases.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"10 1","pages":"412-423"},"PeriodicalIF":3.3,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8451459/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39411255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

FAM13A promotes proliferation of bovine preadipocytes by targeting Hypoxia-Inducible factor-1 signaling pathway. FAM13A通过靶向缺氧诱导因子-1信号通路促进牛前脂肪细胞增殖。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1986327

Chengcheng Liang, Guohua Wang, Sayed Haidar Abbas Raza, Xiaoyu Wang, Bingzhi Li, Wenzhen Zhang, Linsen Zan

{"title":"FAM13A promotes proliferation of bovine preadipocytes by targeting Hypoxia-Inducible factor-1 signaling pathway.","authors":"Chengcheng Liang, Guohua Wang, Sayed Haidar Abbas Raza, Xiaoyu Wang, Bingzhi Li, Wenzhen Zhang, Linsen Zan","doi":"10.1080/21623945.2021.1986327","DOIUrl":"https://doi.org/10.1080/21623945.2021.1986327","url":null,"abstract":"The family with sequence similarity 13 member A (FAM13A) gene has been discovered in recent years and is related to metabolism. In this study, the function of FAM13A in precursor adipocyte proliferation in Qinchuan cattle was investigated using fluorescence quantitative polymerase chain reaction (PCR), western blotting, 5-ethynyl-2'-deoxyuridine staining, and other tests. FAM13A promoted precursor adipocyte proliferation. To determine the pathway FAM13A was involved in, transcriptome sequencing, fluorescence quantitative PCR, western blotting, and other tests were used, which identified the hypoxia inducible factor-1 (HIF-1) signalling pathway. Finally, cobalt chloride, a chemical mimic of hypoxia, was used to treat precursor adipocytes. mRNA and protein levels of FAM13A were significantly increased after hypoxia. Thus, FAM13A promoted bovine precursor adipocyte proliferation by inhibiting the HIF-1 signalling pathway, whereas chemically induced hypoxia negatively regulated FAM13A expression, regulating cell proliferation.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"10 1","pages":"546-557"},"PeriodicalIF":3.3,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8547837/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39536486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

MicroRNA-378 regulates adipogenic differentiation in bovine intramuscular preadipocytes by targeting CaMKK2. MicroRNA-378通过靶向CaMKK2调控牛肌内前脂肪细胞的成脂分化。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1982526

Dongwei Li, Heng Wang, Yongmin Li, Changqing Qu, Yunhai Zhang, Hongyu Liu, Xiaorong Zhang

{"title":"MicroRNA-378 regulates adipogenic differentiation in bovine intramuscular preadipocytes by targeting CaMKK2.","authors":"Dongwei Li, Heng Wang, Yongmin Li, Changqing Qu, Yunhai Zhang, Hongyu Liu, Xiaorong Zhang","doi":"10.1080/21623945.2021.1982526","DOIUrl":"https://doi.org/10.1080/21623945.2021.1982526","url":null,"abstract":"Intramuscular fat, as one of the most important palatability attribute of beef carcase, is the primary determinant of beef quality. The research of adipogenesis mechanism would provide new insight into intramuscular fatty deposition. Here, the role of microRNA-378 was investigated during bovine adipogenic differentiation. It was revealed that miR-378 expression exists variably in bovine major tissue and organs by RT-qPCR. It was predicted that miR-378 targets CaMKK2, as an AMPKα kinase, by DIANA Tools. For better research, primary preadipocytes with stable transfection for up-/down-regulated expression of miR-378 were constructed by lentiviral vectors with GFP gene. The analyses of qPCR showed that PPARγ and adiponectin mRNA levels increased, but C/EBPβ, pref-1 and CaMKK2 mRNA levels decreased during adipogenic differentiation. When miR-378 was overexpressed, preadipocytes proliferation became slower, there are more cellular lipid droplets, and PPARγ and C/EBPβ mRNA levels were higher, but pref-1, adiponectin and CaMKK2 were lower than control groups. Luciferase assay and western blot analysis validated that miR-378 binds the nucleotide sites of the 3'- untranslated region of CaMKK2, which inhibits the mRNA and protein expression of CaMKK2. These findings suggest that miR-378 promotes adipogenic differentiation in bovine intramuscular preadipocytes by targeting CaMKK2 via AMPK signalling pathway.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"10 1","pages":"483-492"},"PeriodicalIF":3.3,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8547835/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39554487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Identification of the transgene insertion site for an adipocyte-specific adiponectin-cre model and characterization of the functional consequences. 确定脂肪细胞特异性脂肪连蛋白-cre模型的转基因插入位点并鉴定其功能后果。

IF 3.5 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1880083

Jared S Farrar, Joseph C Lownik, Grayson W Way, Matthew C Rodriguez, Francesco S Celi, Rebecca K Martin

引用次数: 0

Effects of non-vascularized adipose tissue transplantation on its genetic profile. 非血管化脂肪组织移植对其基因谱的影响。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1889815

Jeannine S Schreiter, L O Kurow, S Langer, M Steinert, L Massier

引用次数: 1

Autologous decellularized extracellular matrix promotes adipogenic differentiation of adipose derived stem cells in low serum culture system by regulating the ERK1/2-PPARγ pathway. 自体脱细胞细胞外基质通过调节ERK1/2-PPARγ通路促进低血清培养系统中脂肪干细胞的成脂分化。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1906509

Yao Qian, Hao Chen, Tianyun Pan, Tian Li, Zikai Zhang, Xuling Lv, Jingping Wang, Ziwan Ji, Yucang He, Liqun Li, Ming Lin

{"title":"Autologous decellularized extracellular matrix promotes adipogenic differentiation of adipose derived stem cells in low serum culture system by regulating the ERK1/2-PPARγ pathway.","authors":"Yao Qian, Hao Chen, Tianyun Pan, Tian Li, Zikai Zhang, Xuling Lv, Jingping Wang, Ziwan Ji, Yucang He, Liqun Li, Ming Lin","doi":"10.1080/21623945.2021.1906509","DOIUrl":"https://doi.org/10.1080/21623945.2021.1906509","url":null,"abstract":"High viability and further adipogenic differentiation of adipose-derived stem cells (ADSCs) are fundamental for engraftment and growth of the transplanted adipose tissue. It has been demonstrated that extracellular matrix (ECM) regulates cell proliferation and differentiation by interacting with ERK1/2 signalling pathway. In this study, we prepared autologous decellularized extracellular matrix (d-ECM) and explored its effect on the proliferation and adipogenic ability of ADSCs in low serum culture. We found that 2% foetal bovine serum (FBS) in growth medium inhibited cell viability and DNA replication, and decreased mRNA and protein levels of PPARγ and C/EPBα compared with 10% FBS. Correspondingly, after 14-days adipogenic induction, cells cultured in 2% FBS possessed lower efficiency of adipogenesis and expressed less adipocyte differentiation markers ADIPOQ and aP2. On the contrary, the d-ECM-coated substrate continuously promoted the expression of PPARγ, and regulated the phosphorylation of ERK1/2 in different manners during differentiation. Pretreatment with ERK1/2 inhibitor PD98059 neutralized the effects of d-ECM, which suggested d-ECM might regulate the adipogenesis of ADSCs through ERK1/2-PPARγ pathway. In addition, d-ECM was revealed to regulate the transcription and expression of stemness-associated genes, such as OCT4, NANOG and SOX2, in the undifferentiated ADSCs, which might be related to the initiation of differentiation.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"10 1","pages":"174-188"},"PeriodicalIF":3.3,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21623945.2021.1906509","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25566711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Measurement of human abdominal and femoral intravascular adipose tissue blood flow using percutaneous Doppler ultrasound. 经皮多普勒超声测量人腹腔和股血管内脂肪组织血流量。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1888471

Ioannis G Lempesis, Gijs H Goossens, Konstantinos N Manolopoulos

引用次数: 7