Adipocyte最新文献_第9页

The paracrine effects of adipocytes on lipid metabolism in doxorubicin-treated triple negative breast cancer cells. 脂肪细胞对阿霉素处理的三阴性乳腺癌细胞脂质代谢的旁分泌作用。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1979758

Ilze Mentoor, Anna-Mart Engelbrecht, Mari van de Vyver, Paul J van Jaarsveld, Theo Nell

{"title":"The paracrine effects of adipocytes on lipid metabolism in doxorubicin-treated triple negative breast cancer cells.","authors":"Ilze Mentoor, Anna-Mart Engelbrecht, Mari van de Vyver, Paul J van Jaarsveld, Theo Nell","doi":"10.1080/21623945.2021.1979758","DOIUrl":"https://doi.org/10.1080/21623945.2021.1979758","url":null,"abstract":"Adipocytes in the breast tumour microenvironment promotes acquired treatment resistance. We used an in vitro adipocyte-conditioned media approach to investigate the direct paracrine effects of adipocyte secretory factors on MDA-MB-231 breast cancer cells treated with doxorubicin to clarify the underlying treatment resistance mechanisms. Cell-viability assays, and Western blots were performed to determine alterations in apoptotic, proliferation and lipid metabolism protein markers. Free fatty acids (FFA) and inflammatory markers in the collected treatment-conditioned media were also quantified. Adipocyte secretory factors increased the cell-viability of doxorubicin-treated cells (p < 0.0001), which did not correspond to apoptosis or proliferation pathways. Adipocyte secretory factors increased the protein expression of hormone-sensitive lipase (p < 0.05) in doxorubicin-treated cells. Adipocyte secretory factors increased the utilization of leptin (p < 0.05) and MCP-1 (p < 0.01) proteins and possibly inhibited release of linoleic acid by doxorubicin-treated cells (treatment-conditioned media FFA profiles). Adipocyte secretory factors induced doxorubicin treatment resistance, by increasing the utilization of inflammatory mediators and inhibiting the release of FFA by doxorubicin-treated cells. This further promotes inflammation and lipid metabolic reprogramming (lipid storage) in the tumour microenvironment, which breast cancer cells use to evade the toxic effects induced by doxorubicin and confers to acquired treatment resistance.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"10 1","pages":"505-523"},"PeriodicalIF":3.3,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8632082/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39917583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Ndufa6 regulates adipogenic differentiation via Scd1. Ndufa6通过Scd1调控成脂分化。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.2007590

Jingwei Zhang, Wei Wang, Ninghan Feng, Xuan Jiang, Shenglong Zhu, Yong Q Chen

引用次数: 10

Matrigel® enhances 3T3-L1 cell differentiation. Matrigel®增强3T3-L1细胞分化。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1951985

Chitmandeep Josan, Sachin Kakar, Sandeep Raha

{"title":"Matrigel® enhances 3T3-L1 cell differentiation.","authors":"Chitmandeep Josan, Sachin Kakar, Sandeep Raha","doi":"10.1080/21623945.2021.1951985","DOIUrl":"10.1080/21623945.2021.1951985","url":null,"abstract":"Culturing cells on bio-gels are believed to provide a more in vivo-like extracellular matrix. 3T3-L1 cells cultured on Matrigel® significantly alteregd their proliferation and differentiation as compared to growth on tissue culture-coated polystyrene surfaces. Growth on a 250-μm thick layer of Matrigel® facilitated the formation of cellular aggregates of 3T3-L1 cells. Differentiation of 3T3-L1 cells cultured on Matrigel® demonstrated increased levels of mRNA levels for key adipogenic transcription factors (PPARγ, C/EBPα, SREBP1), lipogenic markers (FAS, FABP4, LPL, PLIN1) and markers of adipocyte maturity (LEP), compared to cells cultured directly on a polystyrene tissue culture surface. The gene expression of extracellular matrix proteins (FN1, COL1A1, COL4A1, COL6, LAM) was decreased in 3T3-L1 cells cultured on Matrigel®. Furthermore, growth on Matrigel® increased lipid accumulation in 3T3-L1 cells in the presence and absence of rosiglitazone, a thiazolidinedione routinely used to optimize differentiation in these cells. These changes in adipocyte gene expression and lipid accumulation patterns may be a result of the increased cell-cell and cell-ECM interactions occurring on the Matrigel®, a scenario that is more reflective of an in vivo model. Taken together, our data advance the understanding of the value of culturing 3T3-L1 cells on Matrigel®.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"10 1","pages":"361-377"},"PeriodicalIF":3.3,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21623945.2021.1951985","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39205352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Resveratrol supplementation reduces ACE2 expression in human adipose tissue. 补充白藜芦醇可降低人脂肪组织中ACE2的表达。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1965315

Marlies de Ligt, Matthijs K C Hesselink, Johanna Jorgensen, Nicole Hoebers, Ellen E Blaak, Gijs H Goossens

引用次数: 27

Adipocytokines in Graves' orbitopathy and the effect of high-dose corticosteroids. 巴塞杜氏眼眶病中的脂肪细胞因子和大剂量皮质类固醇的作用。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1980258

Jan Schovanek, Michal Krupka, Lubica Cibickova, Marta Karhanova, Sunaina Reddy, Veronika Kucerova, Zdenek Frysak, David Karasek

{"title":"Adipocytokines in Graves' orbitopathy and the effect of high-dose corticosteroids.","authors":"Jan Schovanek, Michal Krupka, Lubica Cibickova, Marta Karhanova, Sunaina Reddy, Veronika Kucerova, Zdenek Frysak, David Karasek","doi":"10.1080/21623945.2021.1980258","DOIUrl":"10.1080/21623945.2021.1980258","url":null,"abstract":"Graves' orbitopathy (GO) is a serious, progressive eye condition seen in patients with autoimmune thyroid disease. GO is characterized by inflammation and swelling of soft orbital tissues. Adipose tissue produces cytokine mediators called adipokines. The present study focuses on the relationship between serum levels of selected adipokines in patients with GO, comparing them with the control group, and uniquely describes the effect of high-dose systemic corticosteroids (HDSC) on their levels. For the purposes of this study, we collected blood samples before and after the treatment with HDSC from 60 GO patients and 34 control subjects and measured serum levels of adiponectin, AIF-1, A-FABP and FGF-21. Levels of adiponectin significantly differed among the three study groups (ANOVA p = 0.03). AIF-1 levels were also significantly different among the study groups (ANOVA p < 0.0001). AIF-1 was significantly associated with the presence of GO after adjusting for clinical factors (age, sex, smoking and BMI) and level of TSH (odds ratio 1.003, p < 0.01). This finding could enforce targeting macrophages in treatment strategies for GO since AIF-1 is considered as a marker of their activation.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"10 1","pages":"456-462"},"PeriodicalIF":3.3,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/34/c5/KADI_10_1980258.PMC8496533.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39480813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cleavage of the vaspin N-terminus releases cell-penetrating peptides that affect early stages of adipogenesis and inhibit lipolysis in mature adipocytes. 血管蛋白n端的分裂释放细胞穿透肽，影响脂肪形成的早期阶段并抑制成熟脂肪细胞的脂肪分解。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1910154

Catherine A Tindall, Estelle Erkner, Jan Stichel, Annette G Beck-Sickinger, Anne Hoffmann, Juliane Weiner, John T Heiker

{"title":"Cleavage of the vaspin N-terminus releases cell-penetrating peptides that affect early stages of adipogenesis and inhibit lipolysis in mature adipocytes.","authors":"Catherine A Tindall, Estelle Erkner, Jan Stichel, Annette G Beck-Sickinger, Anne Hoffmann, Juliane Weiner, John T Heiker","doi":"10.1080/21623945.2021.1910154","DOIUrl":"https://doi.org/10.1080/21623945.2021.1910154","url":null,"abstract":"Vaspin expression and function is related to metabolic disorders and comorbidities of obesity. In various cellular and animal models of obesity, diabetes and atherosclerosis vaspin has shown beneficial, protective and/or compensatory action. While testing proteases for inhibition by vaspin, we noticed specific cleavage within the vaspin N-terminus and sequence analysis predicted cell-penetrating activity for the released peptides. These findings raised the question whether these proteolytic peptides exhibit biological activity.We synthesized various N-terminal vaspin peptides to investigate cell-penetrating activity and analyse uptake mechanisms. Focusing on adipocytes, we performed microarray analysis and functional assays to elucidate biological activities of the vaspin-derived peptide, which is released by KLK7 cleavage (vaspin residues 21-30; VaspinN). Our study provides first evidence that proteolytic processing of the vaspin N-terminus releases cell-penetrating and bioactive peptides with effects on adipocyte biology. The VaspinN peptide increased preadipocyte proliferation, interfered with clonal expansion during the early stage of adipogenesis and blunted adrenergic cAMP-signalling, downstream lipolysis as well as insulin signalling in mature adipocytes.Protease-mediated release of functional N-terminal peptides presents an additional facet of vaspin action. Future studies will address the mechanisms underlying the biological activities and clarify, if vaspin-derived peptides may have potential as therapeutic agents for the treatment of metabolic diseases.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"10 1","pages":"216-231"},"PeriodicalIF":3.3,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21623945.2021.1910154","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38804432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

SENP1 is required for the growth, migration, and survival of human adipose-derived stem cells. SENP1是人类脂肪源性干细胞生长、迁移和存活所必需的。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2020.1863625

Yingying Wu, Beixin Yu, Min Wang

{"title":"SENP1 is required for the growth, migration, and survival of human adipose-derived stem cells.","authors":"Yingying Wu, Beixin Yu, Min Wang","doi":"10.1080/21623945.2020.1863625","DOIUrl":"https://doi.org/10.1080/21623945.2020.1863625","url":null,"abstract":"Human adipose-derived stem cells (hADSCs) are adult mesenchymal cells that have attracted the interest of clinical scientists and surgeons due to their large number of advantages including ease of access and expansion, abundance in cell culture, high proliferative rates, and lower senescence. SUMO/sentrin specific protease 1 (SENP1) is a critical protease that is required during the process of SUMOylation and deSUMOylation, which are dynamic mechanisms that influence cell cycle progression, cell proliferation, and apoptotic status. However, the contribution of SENP1 to these important cellular processes in hADSCs is largely uncharacterized and further studies in this area are required. Here, we show for the first time that after knock out SENP1 in hADSCs, their capacity to migrate and proliferate were inhibited, while apoptosis was enhanced. However, SENP1 did not significantly influence the morphology and MSC-related phenotypes of the hADSCs. These results highlight a role for SENP1 during hADSC growth, and its potential as a therapeutic target to improve the efficacy and safety of hADSCs in the clinic.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"10 1","pages":"38-47"},"PeriodicalIF":3.3,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21623945.2020.1863625","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39094251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

A novel immune-related genes signature after bariatric surgery is histologically associated with non-alcoholic fatty liver disease. 减肥手术后一种新的免疫相关基因特征在组织学上与非酒精性脂肪肝相关。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1970341

Yancheng Song, Jan Zhang, Hexiang Wang, Dong Guo, Chentong Yuan, Bo Liu, Hao Zhong, Dongmei Li, Yu Li

{"title":"A novel immune-related genes signature after bariatric surgery is histologically associated with non-alcoholic fatty liver disease.","authors":"Yancheng Song, Jan Zhang, Hexiang Wang, Dong Guo, Chentong Yuan, Bo Liu, Hao Zhong, Dongmei Li, Yu Li","doi":"10.1080/21623945.2021.1970341","DOIUrl":"https://doi.org/10.1080/21623945.2021.1970341","url":null,"abstract":"Increasing evidence shows that immune-related genes (IRGs) play an important role in bariatric surgery (BS). We identified differentially expressed immune-related genes (DEIRGs) of adipose tissue after BS by analysing the two expression profiles of GEO (GSE59034 and GSE29409). Subsequently, enrichment analysis, GSEA and PPI networks were examined to identify the hub IRGs and related pathways. The performance of the signature was evaluated by area under the curve (AUC) of the receiver operating characteristic (ROC). CIBERSORT algorithm was used to evaluate the relative abundance of infiltrated immune cells.42 DEIRGs were found between the GSE59034 and GSE29409 datasets. The AUC of the signature was 0.904 and 0.865 in the GSE58979 and GSE48452, respectively. Interestingly, the signature also showed good performance in diagnosing non-alcoholic fatty liver disease (NAFLD) (AUC was 0.834 and 0.800, respectively). The number of neutrophils, macrophages M2, macrophages M0 and dendritic cells activated decreased significantly. After BS, the infiltration of T cells regulatory, monocytes, mast cells resting and plasma cells in adipose tissue increased. The novel proposed IRGs signature reveals the underlying immune mechanism of BS and is a promising biomarker for distinguishing the severity of NAFLD. This will provide new insights into strategies for treating obesity and NAFLD.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"10 1","pages":"424-434"},"PeriodicalIF":3.3,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8437528/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39404928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Human antigen R promotes angiogenesis of endothelial cells cultured with adipose stem cells derived exosomes via overexpression of vascular endothelial growth factor in vitro. 人抗原R通过体外过表达血管内皮生长因子促进脂肪干细胞来源外泌体培养的内皮细胞的血管生成。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1982577

Guo Li, Youbai Chen, Yudi Han, Tian Ma, Yan Han

{"title":"Human antigen R promotes angiogenesis of endothelial cells cultured with adipose stem cells derived exosomes via overexpression of vascular endothelial growth factor in vitro.","authors":"Guo Li, Youbai Chen, Yudi Han, Tian Ma, Yan Han","doi":"10.1080/21623945.2021.1982577","DOIUrl":"https://doi.org/10.1080/21623945.2021.1982577","url":null,"abstract":"Recent studies showed that exosomes obtained from adipose-derived stem cells (ADSCs) could improve the angiogenesis of fat grafts via overexpression of vascular endothelial growth factor (VEGF). Human antigen R (HuR) promotes the expression of VEGF in many cancers, but the effect of HuR in normal endothelial cells in the presence of ADSC-derived exosomes remains unclear. We aimed to investigate the effect of HuR on the expression of VEGF and angiogenesis of human umbilical vein endothelial cells (HUVECs) cultured with ADSCs-derived exosomes. The HuR-overexpressed HUVECs (HuR-HUVECs) were cocultured with ADSCs-derived exosomes. qRT-PCR and Western blotting were performed to examine the stability and expression of VEGF-A mRNA and protein. The proliferation, migration, and proangiogenic capacity of HuR-HUVECs were evaluated using cell counting kit-8 (CCK-8), scratch wound healing, and Matrigel tube formation assay. qRT-PCR showed that HuR-HUVECs had higher expression and slower attenuation of VEGF-A mRNA. Western blotting confirmed higher expression of VEGF-A in HuR-HUVECs. CCK-8, scratch wound healing, and Matrigel tube formation assay demonstrated an increased proangiogenic effect in HuR-HUVECs. HuR promotes angiogenesis of HUVECs cocultured with ADSCs-derived exosomes via stabilization and overexpression of VEGF in vitro. The HuR/VEGF pathway is an important regulatory mechanism of angiogenesis in endothelial cells.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"10 1","pages":"475-482"},"PeriodicalIF":3.3,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8510607/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39506003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Dysfunctional adiposity index as a marker of adipose tissue morpho-functional abnormalities and metabolic disorders in apparently healthy subjects. 功能失调肥胖指数作为表面健康受试者脂肪组织形态功能异常和代谢紊乱的标志。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1893452

Juan Reyes-Barrera, Victor H Sainz-Escárrega, Aida X Medina-Urritia, Esteban Jorge-Galarza, Horacio Osorio-Alonso, Margarita Torres-Tamayo, Gabriela Leal-Escobar, Carlos Posadas-Romero, Ivan Torre-Villalvazo, Juan G Juárez-Rojas

{"title":"Dysfunctional adiposity index as a marker of adipose tissue morpho-functional abnormalities and metabolic disorders in apparently healthy subjects.","authors":"Juan Reyes-Barrera, Victor H Sainz-Escárrega, Aida X Medina-Urritia, Esteban Jorge-Galarza, Horacio Osorio-Alonso, Margarita Torres-Tamayo, Gabriela Leal-Escobar, Carlos Posadas-Romero, Ivan Torre-Villalvazo, Juan G Juárez-Rojas","doi":"10.1080/21623945.2021.1893452","DOIUrl":"https://doi.org/10.1080/21623945.2021.1893452","url":null,"abstract":"Compared to body mass index, waist circumference (WC), and adiposity measurements, adipose tissue (AT) morpho-functionality evaluations are better predictors of cardiometabolic abnormalities (CA). The present study establishes a dysfunctional adiposity index (DAI) as an early marker of CA based on adipocytes morpho-functional abnormalities. DAI was established in 340 subjects without cardiovascular risk factors selected from a cross-sectional study (n=1600). Then, DAI was calculated in 36 healthy subjects who underwent subcutaneous AT biopsy. The correlation of DAI with adipocyte morphology (size/number) and functionality (adiponectin/leptin ratio) was analyzed. The DAI cut-off point was identified and its independent association with CA was determined in 1418 subjects from the cross-sectional study. The constant parameters to calculate the DAI were [WC/[22.79+[2.68*BMI]]]*[triglycerides (TG, mmol/L)/1.37]*[1.19/high density lipoprotein-cholesterol (HDL-C, mmol/L)] for males, and [WC/[24.02+[2.37*BMI]]]*[TG(mmol/L)/1.32]*[1.43/HDL-C(mmol/L)] for females. DAI correlated with adipocytes mean area, adipocyte number and adiponectin/leptin ratio. DAI ≥1.065 was independently associated with diabetes, non-alcoholic fatty liver disease, subclinical atherosclerosis, and hypertension. The present study highlights that DAI is associated with early CA independently of adiposity and other risk factors. Since DAI is obtained using accessible parameters, it can be easily incorporated into clinical practice for early identification of AT abnormalities in apparently healthy subjects.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"10 1","pages":"142-152"},"PeriodicalIF":3.3,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21623945.2021.1893452","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25480696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17