Adipocyte最新文献_第8页

Hematopoietic stem cells produce intermediate lineage adipocyte progenitors that simultaneously express both myeloid and mesenchymal lineage markers in adipose tissue. 造血干细胞产生中间谱系脂肪祖细胞，同时表达脂肪组织中的髓系和间充质谱系标记。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1957290

Kathleen M Gavin, Timothy M Sullivan, Joanne K Maltzahn, Jeremy T Rahkola, Alistair S Acosta, Wendy M Kohrt, Susan M Majka, Dwight J Klemm

{"title":"Hematopoietic stem cells produce intermediate lineage adipocyte progenitors that simultaneously express both myeloid and mesenchymal lineage markers in adipose tissue.","authors":"Kathleen M Gavin, Timothy M Sullivan, Joanne K Maltzahn, Jeremy T Rahkola, Alistair S Acosta, Wendy M Kohrt, Susan M Majka, Dwight J Klemm","doi":"10.1080/21623945.2021.1957290","DOIUrl":"https://doi.org/10.1080/21623945.2021.1957290","url":null,"abstract":"Some adipocytes are produced from bone marrow hematopoietic stem cells. In vitro studies previously indicated that these bone marrow-derived adipocytes (BMDAs) were generated from adipose tissue macrophage (ATM) that lose their hematopoietic markers and acquire mesenchymal markers prior to terminal adipogenic differentiation. Here we interrogated whether this hematopoietic-to-mesenchymal transition drives BMDA production In vitro. We generated transgenic mice in which the lysozyme gene promoter (LysM) indelibly labeled ATM with green fluorescent protein (GFP). We discovered that adipose stroma contained a population of LysM-positive myeloid cells that simultaneously expressed hematopoietic/myeloid markers (CD45 and CD11b), and mesenchymal markers (CD29, PDGFRa and Sca-1) typically found on conventional adipocyte progenitors. These cells were capable of adipogenic differentiation In vitro and In vitro, while other stromal populations deficient in PDGFRa and Sca-1 were non-adipogenic. BMDAs and conventional adipocytes expressed common fat cell markers but exhibited little or no expression of hematopoietic and mesenchymal progenitor cell markers. The data indicate that BMDAs are produced from ATM simultaneously expressing hematopoietic and mesenchymal markers rather than via a stepwise hematopoietic-to-mesenchymal transition. Because BMDA production is stimulated by high fat feeding, their production from hematopoietic progenitors may maintain adipocyte production when conventional adipocyte precursors are diminished.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"10 1","pages":"394-407"},"PeriodicalIF":3.3,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8381847/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39320618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Association between rs174537 FADS1 polymorphism and immune cell profiles in abdominal and femoral subcutaneous adipose tissue: an exploratory study in adults with obesity. rs174537 FADS1多态性与腹部和股皮下脂肪组织免疫细胞谱之间的关系:一项针对肥胖成人的探索性研究

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1888470

Chenxuan Wang, Jessica Murphy, Kerri Z Delaney, Natalie Khor, José A Morais, Michael A Tsoukas, Dana E Lowry, David M Mutch, Sylvia Santosa

{"title":"Association between rs174537 FADS1 polymorphism and immune cell profiles in abdominal and femoral subcutaneous adipose tissue: an exploratory study in adults with obesity.","authors":"Chenxuan Wang, Jessica Murphy, Kerri Z Delaney, Natalie Khor, José A Morais, Michael A Tsoukas, Dana E Lowry, David M Mutch, Sylvia Santosa","doi":"10.1080/21623945.2021.1888470","DOIUrl":"https://doi.org/10.1080/21623945.2021.1888470","url":null,"abstract":"Fatty acid desaturase 1 (FADS1) polymorphisms alter fatty acid content in subcutaneous adipose tissue (SAT); however, existing evidence is limited and conflicting regarding the association between FADS1 variants and SAT inflammatory status. To advance this area, we conducted an exploratory study to investigate whether the common rs174537 polymorphism in FADS1 was associated with immune cell profiles in abdominal and femoral SAT in individuals with obesity. FADS1 gene expression and immune cell profiles in SAT depots were assessed by qPCR and flow cytometry, respectively. Although FADS1 gene expression was associated with genotype, no associations were observed with immune cell profiles in either depot. Our study provides additional evidence that rs174537 in FADS1 has minimal impact on inflammatory status in obese SAT.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"10 1","pages":"124-130"},"PeriodicalIF":3.3,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21623945.2021.1888470","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25380108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Verapamil induces autophagy to improve liver regeneration in non-alcoholic fatty liver mice. 维拉帕米诱导自噬促进非酒精性脂肪肝小鼠肝脏再生。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1983241

Jian-Lin Lai, Yuan-E Lian, Jun-Yi Wu, Yao-Dong Wang, Yan-Nan Bai

{"title":"Verapamil induces autophagy to improve liver regeneration in non-alcoholic fatty liver mice.","authors":"Jian-Lin Lai, Yuan-E Lian, Jun-Yi Wu, Yao-Dong Wang, Yan-Nan Bai","doi":"10.1080/21623945.2021.1983241","DOIUrl":"10.1080/21623945.2021.1983241","url":null,"abstract":"Verapamil can restore intracellular calcium homeostasis, increase the fusion of autophagosomes and lysosomes, reduce lipid droplet accumulation and inhibit inflammation and insulin resistance in high-fat-fed mice. The present study aimed to investigate verapamil's effect and its underlying liver regeneration mechanism in mice with non-alcoholic fatty liver. After 50% hepatectomy was performed, the changes of autophagy and liver regeneration were evaluated by detecting cell proliferation and autophagy at each time point. Then, 25mg/kg verapamil was injected intraperitoneally for 10 d before an operation in the mild to moderate fatty liver and severe fatty liver groups. The control group and mild to moderate fatty liver group reached the peak of proliferation at 24-48h after operation, and the mice with severe fatty liver and steatohepatitis reached the peak at 48-72h. Autophagy in the normal group and mild to moderate fatty liver group reached the peak 48 hours after operation. Verapamil injection can enhance autophagy, reduce the weight of fatty liver mice, improve liver function and liver regeneration. Verapamil can induce autophagy, improve hepatocyte function and promote hepatocyte regeneration through the mTOR independent signaling pathway, thus improving the process of liver regeneration after partial hepatectomy.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"10 1","pages":"532-545"},"PeriodicalIF":3.3,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8555529/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39558763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Composition, isolation, identification and function of adipose tissue-derived exosomes. 脂肪组织源性外泌体的组成、分离、鉴定和功能。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1983242

Rui Zhao, Tiantian Zhao, Zhaozhao He, Rui Cai, Weijun Pang

{"title":"Composition, isolation, identification and function of adipose tissue-derived exosomes.","authors":"Rui Zhao, Tiantian Zhao, Zhaozhao He, Rui Cai, Weijun Pang","doi":"10.1080/21623945.2021.1983242","DOIUrl":"https://doi.org/10.1080/21623945.2021.1983242","url":null,"abstract":"Exosomes are nano-sized extracellular vesicles (30-160 nm diameter) with lipid bilayer membrane secrete by various cells that mediate the communication between cells and tissue, which contain a variety of non-coding RNAs, mRNAs, proteins, lipids and other functional substances. Adipose tissue is important energy storage and endocrine organ in the organism. Recent studies have revealed that adipose tissue-derived exosomes (AT-Exosomes) play a critical role in many physiologically and pathologically functions. Physiologically, AT-Exosomes could regulate the metabolic homoeostasis of various organs or cells including liver and skeletal muscle. Pathologically, they could be used in the treatment of disease and or that they may be involved in the progression of the disease. In this review, we describe the basic principles and methods of exosomes isolation and identification, as well as further summary the specific methods. Moreover, we categorize the relevant studies of AT-Exosomes and summarize the different components and biological functions of mammalian exosomes. Most importantly, we elaborate AT-Exosomes crosstalk within adipose tissue and their functions on other tissues or organs from the physiological and pathological perspective. Based on the above analysis, we discuss what remains to be discovered problems in AT-Exosomes studies and prospect their directions needed to be further explored in the future.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"10 1","pages":"587-604"},"PeriodicalIF":3.3,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8604391/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39570672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Application of a deep learning-based image analysis and live-cell imaging system for quantifying adipogenic differentiation kinetics of adipose-derived stem/stromal cells. 应用基于深度学习的图像分析和活细胞成像系统定量脂肪源性干细胞/基质细胞的成脂分化动力学。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.2000696

Patrick Terrence Brooks, Lea Munthe-Fog, Klaus Rieneck, Frederik Banch Clausen, Olga Ballesteros Rivera, Eva Kannik Haastrup, Anne Fischer-Nielsen, Jesper Dyrendom Svalgaard

{"title":"Application of a deep learning-based image analysis and live-cell imaging system for quantifying adipogenic differentiation kinetics of adipose-derived stem/stromal cells.","authors":"Patrick Terrence Brooks, Lea Munthe-Fog, Klaus Rieneck, Frederik Banch Clausen, Olga Ballesteros Rivera, Eva Kannik Haastrup, Anne Fischer-Nielsen, Jesper Dyrendom Svalgaard","doi":"10.1080/21623945.2021.2000696","DOIUrl":"https://doi.org/10.1080/21623945.2021.2000696","url":null,"abstract":"ABSTRACT Quantitative methods for assessing differentiative potency of adipose-derived stem/stromal cells may lead to improved clinical application of this multipotent stem cell, by advancing our understanding of specific processes such as adipogenic differentiation. Conventional cell staining methods are used to determine the formation of adipose areas during adipogenesis as a qualitative representation of adipogenic potency. Staining methods such as oil-red-O are quantifiable using absorbance measurements, but these assays are time and material consuming. Detection methods for cell characteristics using advanced image analysis by machine learning are emerging. Here, live-cell imaging was combined with a deep learning-based detection tool to quantify the presence of adipose areas and lipid droplet formation during adipogenic differentiation of adipose-derived stem/stromal cells. Different detection masks quantified adipose area and lipid droplet formation at different time points indicating kinetics of adipogenesis and showed differences between individual donors. Whereas CEBPA and PPARG expression seems to precede the increase in adipose area and lipid droplets, it might be able to predict expression of ADIPOQ. The applied method is a proof of concept, demonstrating that deep learning methods can be used to investigate adipogenic differentiation and kinetics in vitro using specific detection masks based on algorithm produced from annotation of image data.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"10 1","pages":"621-630"},"PeriodicalIF":3.3,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8632106/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39705456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Staufen1 unwinds the secondary structure and facilitates the translation of fatty acid binding protein 4 mRNA during adipogenesis. 在脂肪形成过程中，Staufen1解开二级结构，促进脂肪酸结合蛋白4mrna的翻译。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1948165

Xiaodi Liang, Yi Jiao, Xueli Gong, Hao Gu, Nuerbiye Nuermaimaiti, Xuanyu Meng, Dihui Liu, Yaqun Guan

{"title":"Staufen1 unwinds the secondary structure and facilitates the translation of fatty acid binding protein 4 mRNA during adipogenesis.","authors":"Xiaodi Liang, Yi Jiao, Xueli Gong, Hao Gu, Nuerbiye Nuermaimaiti, Xuanyu Meng, Dihui Liu, Yaqun Guan","doi":"10.1080/21623945.2021.1948165","DOIUrl":"https://doi.org/10.1080/21623945.2021.1948165","url":null,"abstract":"Adipogenesis is regulated by genetic interactions, in which post-transcriptional regulation plays an important role. Staufen double-stranded RNA binding protein 1 (Staufen1 or STAU1) plays diverse roles in RNA processing and adipogenesis. Previously, we found that the downregulation of STAU1 affects the expression of fatty acid-binding protein 4 (FABP4) at the protein level but not at the mRNA level. This study aimed to determine the mechanism underlying the regulation of FABP4 expression by STAU1, explaining the inconsistency between FABP4 mRNA and protein levels. We used RNA interference, photoactivatable ribonucleoside enhanced cross-linking and immunoprecipitation, and an adeno-associated virus to examine the functions of STAU1 in adipogenesis. Our results indicate that STAU1 binds to the coding sequences of FABP4, thereby regulating the translation of FABP4 mRNA by unwinding the double-stranded structure. Furthermore, STAU1 mediates adipogenesis by regulating the secretion of free fatty acids. However, STAU1 knockdown decreases the fat weight/body weight ratio but does not affect the plasma triglyceride levels. These findings describe the mechanisms involved in STAU1-mediated regulation of FABP4 expression at the translational level during adipogenesis.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"10 1","pages":"350-360"},"PeriodicalIF":3.3,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21623945.2021.1948165","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39151741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The paracrine effects of adipocytes on lipid metabolism in doxorubicin-treated triple negative breast cancer cells. 脂肪细胞对阿霉素处理的三阴性乳腺癌细胞脂质代谢的旁分泌作用。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1979758

Ilze Mentoor, Anna-Mart Engelbrecht, Mari van de Vyver, Paul J van Jaarsveld, Theo Nell

{"title":"The paracrine effects of adipocytes on lipid metabolism in doxorubicin-treated triple negative breast cancer cells.","authors":"Ilze Mentoor, Anna-Mart Engelbrecht, Mari van de Vyver, Paul J van Jaarsveld, Theo Nell","doi":"10.1080/21623945.2021.1979758","DOIUrl":"https://doi.org/10.1080/21623945.2021.1979758","url":null,"abstract":"Adipocytes in the breast tumour microenvironment promotes acquired treatment resistance. We used an in vitro adipocyte-conditioned media approach to investigate the direct paracrine effects of adipocyte secretory factors on MDA-MB-231 breast cancer cells treated with doxorubicin to clarify the underlying treatment resistance mechanisms. Cell-viability assays, and Western blots were performed to determine alterations in apoptotic, proliferation and lipid metabolism protein markers. Free fatty acids (FFA) and inflammatory markers in the collected treatment-conditioned media were also quantified. Adipocyte secretory factors increased the cell-viability of doxorubicin-treated cells (p < 0.0001), which did not correspond to apoptosis or proliferation pathways. Adipocyte secretory factors increased the protein expression of hormone-sensitive lipase (p < 0.05) in doxorubicin-treated cells. Adipocyte secretory factors increased the utilization of leptin (p < 0.05) and MCP-1 (p < 0.01) proteins and possibly inhibited release of linoleic acid by doxorubicin-treated cells (treatment-conditioned media FFA profiles). Adipocyte secretory factors induced doxorubicin treatment resistance, by increasing the utilization of inflammatory mediators and inhibiting the release of FFA by doxorubicin-treated cells. This further promotes inflammation and lipid metabolic reprogramming (lipid storage) in the tumour microenvironment, which breast cancer cells use to evade the toxic effects induced by doxorubicin and confers to acquired treatment resistance.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"10 1","pages":"505-523"},"PeriodicalIF":3.3,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8632082/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39917583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Ndufa6 regulates adipogenic differentiation via Scd1. Ndufa6通过Scd1调控成脂分化。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.2007590

Jingwei Zhang, Wei Wang, Ninghan Feng, Xuan Jiang, Shenglong Zhu, Yong Q Chen

引用次数: 10

Matrigel® enhances 3T3-L1 cell differentiation. Matrigel®增强3T3-L1细胞分化。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1951985

Chitmandeep Josan, Sachin Kakar, Sandeep Raha

{"title":"Matrigel® enhances 3T3-L1 cell differentiation.","authors":"Chitmandeep Josan, Sachin Kakar, Sandeep Raha","doi":"10.1080/21623945.2021.1951985","DOIUrl":"10.1080/21623945.2021.1951985","url":null,"abstract":"Culturing cells on bio-gels are believed to provide a more in vivo-like extracellular matrix. 3T3-L1 cells cultured on Matrigel® significantly alteregd their proliferation and differentiation as compared to growth on tissue culture-coated polystyrene surfaces. Growth on a 250-μm thick layer of Matrigel® facilitated the formation of cellular aggregates of 3T3-L1 cells. Differentiation of 3T3-L1 cells cultured on Matrigel® demonstrated increased levels of mRNA levels for key adipogenic transcription factors (PPARγ, C/EBPα, SREBP1), lipogenic markers (FAS, FABP4, LPL, PLIN1) and markers of adipocyte maturity (LEP), compared to cells cultured directly on a polystyrene tissue culture surface. The gene expression of extracellular matrix proteins (FN1, COL1A1, COL4A1, COL6, LAM) was decreased in 3T3-L1 cells cultured on Matrigel®. Furthermore, growth on Matrigel® increased lipid accumulation in 3T3-L1 cells in the presence and absence of rosiglitazone, a thiazolidinedione routinely used to optimize differentiation in these cells. These changes in adipocyte gene expression and lipid accumulation patterns may be a result of the increased cell-cell and cell-ECM interactions occurring on the Matrigel®, a scenario that is more reflective of an in vivo model. Taken together, our data advance the understanding of the value of culturing 3T3-L1 cells on Matrigel®.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"10 1","pages":"361-377"},"PeriodicalIF":3.3,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21623945.2021.1951985","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39205352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Resveratrol supplementation reduces ACE2 expression in human adipose tissue. 补充白藜芦醇可降低人脂肪组织中ACE2的表达。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1965315

Marlies de Ligt, Matthijs K C Hesselink, Johanna Jorgensen, Nicole Hoebers, Ellen E Blaak, Gijs H Goossens

引用次数: 27