Adipocyte最新文献_第7页

The combination of nuclear receptor NR1D1 and ULK1 promotes mitophagy in adipocytes to ameliorate obesity 核受体NR1D1和ULK1的组合促进脂肪细胞的线粒体自噬以改善肥胖

IF 3.3 4区生物学

Adipocyte Pub Date : 2022-04-11 DOI: 10.1080/21623945.2022.2060719

Bo Yu, Jindun Pan, F. Yu

{"title":"The combination of nuclear receptor NR1D1 and ULK1 promotes mitophagy in adipocytes to ameliorate obesity","authors":"Bo Yu, Jindun Pan, F. Yu","doi":"10.1080/21623945.2022.2060719","DOIUrl":"https://doi.org/10.1080/21623945.2022.2060719","url":null,"abstract":"ABSTRACT Obesity is a severe disease worldwide. Mitochondrial autophagy (mitophagy) may be related to metabolic abnormalities in obese individuals, but the mechanism is still unclear. We aimed to investigate whether nuclear receptors NR1D1 and ULK1 influence obesity by affecting mitophagy. In vitro model was established by inducing 3T3-L1 cells differentiation. MTT was detected cell viability. ELISA was tested triglyceride (TG). Oil red O staining was performed to detect lipid droplets. Flow cytometry was measured mtROS. ChIP and Dual-luciferase reporter assay were verified NR1D1 bind to ULK1. LC3 level was detected by IF. After differentiation medium treatment, cell viability was decreased, TG content and lipid droplets were increased Moreover, NR1D1 expression was reduced in Model group. NR1D1 overexpression was increased cell viability, reduced TG content and lipid droplets. Subsequently, NR1D1 inhibited TOM20 and mtROS, whereas, Parkin and PINK1 were accelerated. NR1D1 overexpression facilitated LC3 expression, whereas ULK1 knockdown was reversed the effect of NR1D1 overexpression. Liensinine also reversed the effect of NR1D1 overexpression, that is, cell viability was reduced, mtROS, TG content and lipid droplets were increased. The combination of nuclear receptor NR1D1 and ULK1 promoted mitophagy in adipocytes to alleviate obesity, which provided new target and strategy for obesity treatment. Abbreviations: Mitochondrial autophagy (mitophagy), triglyceride (TG), Uncoordinated-51 like autophagy activating kinase 1 (ULK1), Nuclear receptor subfamily 1 group D member 1 (NR1D1), American Type Culture Collection (ATCC), fetal bovine serum (FBS), 3-isobutyl-1-methylxanthine (IBMX), dexamethasone (DEX), short hairpin RNA ULK1 (sh-ULK1), wild-type (WT), mutant (MUT), Enzyme-linked immunosorbent assay (ELISA), mitochondrial reactive oxygen species (mtROS), Chromatin immunoprecipitation (ChIP), Quantitative real-time PCR (qRT-PCR), Immunofluorescence (IF), standard deviation (SD).","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"11 1","pages":"202 - 212"},"PeriodicalIF":3.3,"publicationDate":"2022-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48301535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

An organoid model derived from human adipose stem/progenitor cells to study adipose tissue physiology 从人脂肪干细胞/祖细胞衍生的类器官模型用于研究脂肪组织生理学

IF 3.3 4区生物学

Adipocyte Pub Date : 2022-03-17 DOI: 10.1080/21623945.2022.2044601

M. Mandl, H. Viertler, Florian M Hatzmann, Camille Brucker, Sonja Großmann, Petra Waldegger, T. Rauchenwald, M. Mattesich, M. Zwierzina, G. Pierer, W. Zwerschke

{"title":"An organoid model derived from human adipose stem/progenitor cells to study adipose tissue physiology","authors":"M. Mandl, H. Viertler, Florian M Hatzmann, Camille Brucker, Sonja Großmann, Petra Waldegger, T. Rauchenwald, M. Mattesich, M. Zwierzina, G. Pierer, W. Zwerschke","doi":"10.1080/21623945.2022.2044601","DOIUrl":"https://doi.org/10.1080/21623945.2022.2044601","url":null,"abstract":"ABSTRACT We established a functional adipose organoid model system for human adipose stem/progenitor cells (ASCs) isolated from white adipose tissue (WAT). ASCs were forced to self-aggregate by a hanging-drop technique. Afterwards, spheroids were transferred into agar-coated cell culture dishes to avoid plastic-adherence and dis-aggregation. Adipocyte differentiation was induced by an adipogenic hormone cocktail. Morphometric analysis revealed a significant increase in organoid size in the course of adipogenesis until d 18. Whole mount staining of organoids using specific lipophilic dyes showed large multi- and unilocular fat deposits in differentiated cells indicating highly efficient differentiation of ASCs into mature adipocytes. Moreover, we found a strong induction of the expression of key adipogenesis and adipocyte markers (CCAAT/enhancer-binding protein (C/EBP) β, peroxisome proliferator-activated receptor (PPAR) γ, fatty acid-binding protein 4 (FABP4), adiponectin) during adipose organoid formation. Secreted adiponectin was detected in the cell culture supernatant, underscoring the physiological relevance of mature adipocytes in the organoid model. Moreover, colony formation assays of collagenase-digested organoids revealed the maintenance of a significant fraction of ASCs within newly formed organoids. In conclusion, we provide a reliable and highly efficient WAT organoid model, which enables accurate analysis of cellular and molecular markers of adipogenic differentiation and adipocyte physiology.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"11 1","pages":"164 - 174"},"PeriodicalIF":3.3,"publicationDate":"2022-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47339781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Adipose tissue aging partially accounts for fat alterations in HIV lipodystrophy 脂肪组织老化部分解释了HIV脂肪营养不良中脂肪的改变

IF 3.3 4区生物学

Adipocyte Pub Date : 2022-03-17 DOI: 10.1080/21623945.2022.2042962

P. Domingo, M. Giralt, A. Gavaldà-Navarro, A. Blasco-Roset, Alejandro Delgado-Anglés, J. M. Gallego-Escuredo, M. Gutiérrez, M. Mateo, R. Cereijo, J. Domingo, F. Villarroya, J. Villarroya

引用次数: 1

Favorable fatty acid composition in adipose tissue in healthy Iraqi- compared to Swedish-born men — a pilot study using MRI assessment 健康伊拉克男性与瑞典出生男性脂肪组织中有利的脂肪酸成分——一项使用MRI评估的初步研究

IF 3.3 4区生物学

Adipocyte Pub Date : 2022-03-16 DOI: 10.1080/21623945.2022.2042963

L. Trinh, Karin G. Stenkula, L. Olsson, J. Svensson, P. Peterson, L. Bennet, S. Månsson

{"title":"Favorable fatty acid composition in adipose tissue in healthy Iraqi- compared to Swedish-born men — a pilot study using MRI assessment","authors":"L. Trinh, Karin G. Stenkula, L. Olsson, J. Svensson, P. Peterson, L. Bennet, S. Månsson","doi":"10.1080/21623945.2022.2042963","DOIUrl":"https://doi.org/10.1080/21623945.2022.2042963","url":null,"abstract":"ABSTRACT Middle Eastern immigrants are at high-risk for insulin resistance. Fatty acid composition (FAC) plays an important role in the development of insulin resistance but has not been investigated in people of Middle Eastern ancestry. Here, the aim was to assess the FAC in visceral and subcutaneous adipose tissue (VAT and SAT) in healthy Iraqi- and Swedish-born men using a magnetic resonance imaging (MRI) method.This case-control study included 23 Iraqi- and 15 Swedish-born middle-aged men, without cardiometabolic disease. Using multi-echo MRI of the abdomen, the fractions of saturated, monounsaturated, and polyunsaturated fatty acids (fSFA, fMUFA, and fPUFA) were estimated in VAT and SAT. SAT was further analyzed in deep and superficial compartments (dSAT and sSAT). In all depots, fPUFA was significantly higher and fSFA significantly lower in Iraqi men, independently of age and BMI. In both Iraqi- and Swedish-born men, higher fPUFA and lower fMUFA were found in sSAT vs. dSAT. Among Iraqi men only, higher fPUFA and lower fMUFA were found in SAT vs. VAT.Iraqi-born men presented a more favorable abdominal FAC compared to Swedish-born men. This MRI method also revealed different FACs in different abdominal depots. Our results may reflect a beneficial FAC in Middle Eastern immigrants.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"11 1","pages":"153 - 163"},"PeriodicalIF":3.3,"publicationDate":"2022-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43917480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

T-stage-specific abdominal visceral fat, haematological nutrition indicators and inflammation as prognostic factors in patients with clear renal cell carcinoma 明确肾细胞癌患者t期特异性腹部内脏脂肪、血液学营养指标和炎症作为预后因素

IF 3.3 4区生物学

Adipocyte Pub Date : 2022-03-12 DOI: 10.1080/21623945.2022.2048546

Hao Guo, Yumei Zhang, Heng Ma, Peiyou Gong, Yinghong Shi, Wenlei Zhao, Ai-yih Wang, Ming Liu, Zehua Sun, Fang Wang, Qing Wang, Xinru Ba

{"title":"T-stage-specific abdominal visceral fat, haematological nutrition indicators and inflammation as prognostic factors in patients with clear renal cell carcinoma","authors":"Hao Guo, Yumei Zhang, Heng Ma, Peiyou Gong, Yinghong Shi, Wenlei Zhao, Ai-yih Wang, Ming Liu, Zehua Sun, Fang Wang, Qing Wang, Xinru Ba","doi":"10.1080/21623945.2022.2048546","DOIUrl":"https://doi.org/10.1080/21623945.2022.2048546","url":null,"abstract":"ABSTRACT Clear cell renal carcinoma (ccRCC) is the most common histological type of renal cancer and has the highest mortality. Several studies have been conducted on the relationship between adipose tissue and ccRCC prognosis, however, the results have been inconsistent to date. The current study aimed at establishing a link between abdominal fat composition and short-term prognosis in patients with ccRCC after T-stage stratification. We retrospectively analysed 250 patients with pathologically confirmed ccRCC (173 low T-stage and 77 high T-stage) in our hospital. The computed tomography (CT) images were evaluated using ImageJ. Then, subcutaneous and visceral fat areas (SFA and VFA), total fat areas (TFA) and the relative VFA (rVFA) were measured and computed. Meanwhile, biochemical indices of blood serum were analysed. The results showed that rVFA in low T-stage cohort who had a history of short-term postoperative complications were significantly lower than those who did not. No such association was observed in the high T-stage cohort. Further investigation revealed that the correlations between biochemical indexes and fat area-related variables varied across T-stage groups. As a result, rVFA is a reliable independent predictor of short-term prognosis in patients with low T-stage ccRCC but not in patients with high T-stage ccRCC.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"11 1","pages":"133 - 142"},"PeriodicalIF":3.3,"publicationDate":"2022-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42934459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Fat grafting and platelet-rich plasma in wound healing: a review of histology from animal studies. 脂肪移植和富血小板血浆在伤口愈合中的应用:动物研究的组织学综述。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1876374

Grant S Nolan, Oliver J Smith, Gavin Jell, Afshin Mosahebi

引用次数: 15

The characterization of metabolites alterations in white adipose tissue of diabetic GK Rats after ileal transposition surgery by an untargeted metabolomics approach. 非靶向代谢组学方法表征糖尿病GK大鼠回肠转位手术后白色脂肪组织中代谢物的变化。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1926139

Xiaorui Lyu, Kemin Yan, Weijie Chen, Yujie Wang, Huijuan Zhu, Hui Pan, Guole Lin, Linjie Wang, Hongbo Yang, Fengying Gong

{"title":"The characterization of metabolites alterations in white adipose tissue of diabetic GK Rats after ileal transposition surgery by an untargeted metabolomics approach.","authors":"Xiaorui Lyu, Kemin Yan, Weijie Chen, Yujie Wang, Huijuan Zhu, Hui Pan, Guole Lin, Linjie Wang, Hongbo Yang, Fengying Gong","doi":"10.1080/21623945.2021.1926139","DOIUrl":"https://doi.org/10.1080/21623945.2021.1926139","url":null,"abstract":"Dysfunction of adipose tissue could lead to insulin resistance, obesity and type 2 diabetes. Thus, our present study aimed to investigate metabolites alterations in white adipose tissue (WAT) of diabetic GK rats after IT surgery. Ten-week-old male diabetic GK rats were randomly subjected to IT and Sham-IT surgery. Six weeks later, the untargeted metabolomics in WAT of diabetic GK rats was performed. Differential metabolites were selected according to the coefficient of variation (CV) of quality control (QC) sample <30%, variable importance in the projection (VIP) >1 and P < 0.05. Then, the hierarchical clustering of differential metabolites was conducted and the KEGG database was used for metabolic pathway analysis. A total of 50 (in positive ion mode) and 68 (in negative ion mode) metabolites were identified as differential metabolites in WAT of diabetic GK rats between IT group and Sham-IT group, respectively. These differential metabolites were well clustered, which in descending order of the number of involved differential metabolites is ubiquinone and other terpenoid-quinone biosynthesis, AMPK signalling pathway, pantothenate and CoA biosynthesis, ferroptosis, vitamin digestion and absorption, glycerophospholipid metabolism, phenylalanine metabolism, steroid hormone biosynthesis, neuroactive ligand-receptor interaction, porphyrin and chlorophyll metabolism and bile secretion, and correlated with the parameters of body weight, food intake, WAT mass and glucose metabolism, which were significantly improved after IT surgery. The differential metabolites in WAT of diabetic GK rats were mainly related to the pathway of energy metabolism, and correlated with the improved phenotypes of diabetic GK rats after IT surgery.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"10 1","pages":"275-284"},"PeriodicalIF":3.3,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21623945.2021.1926139","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38889495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) influences adipocytes injuries through triggering XBP1 and activating mitochondria-mediated apoptosis. 牙龈卟啉单胞菌脂多糖(Pg-LPS)通过触发XBP1和激活线粒体介导的细胞凋亡影响脂肪细胞损伤。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2020.1856527

Ying-Tao Lv, Jin-Jin Zeng, Jia-Yi Lu, Xue-Yang Zhang, Ping-Ping Xu, Yuan Su

{"title":"Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) influences adipocytes injuries through triggering XBP1 and activating mitochondria-mediated apoptosis.","authors":"Ying-Tao Lv, Jin-Jin Zeng, Jia-Yi Lu, Xue-Yang Zhang, Ping-Ping Xu, Yuan Su","doi":"10.1080/21623945.2020.1856527","DOIUrl":"https://doi.org/10.1080/21623945.2020.1856527","url":null,"abstract":"Obesity is an important public-health problem worldwide. This study aimed to determine effects of porphyromonas gingivalis lipopolysaccharide (Pg-LPS) on adipocytes injuries and explore associated mechanisms. Adipocytes were isolated from SD rats. pLVX-XBP1 (XBP1 over-expression) and pLVX-XBP1-RNAi (silencing XBP1) were structured and transfected into adipocytes. All adipocytes were divided into pLVX-NC, pLVX-XBP1, pLVX-NC+Pg-LPS and pLVX-XBP1+ Pg-LPS group. Oil-Red O staining was employed to identify isolated adipocytes. Quantitative real-time PCR (qRT-PCR) was used to examine gene transcription of IL-6, TNF-α, leptin, adiponectin. Western blotting was used to detect Bax and caspase-3 expression. Adipocytes were successfully isolated and identified with Oil-Red O staining. Both XBP1 mimic and XBP1 RNAi were effectively transfected into adipocytes with higher expressing efficacy. XBP1 over-expression significantly aggravated Pg-LPS induced inflammatory response compared to adipocytes without Pg-LPS treatment (p<0.05). Pg-LPS significantly enhanced leptin and inhibited adiponectin expression by up-regulating XBP1 expression (p<0.05). XBP1 silence significantly alleviated Pg-LPS induced inflammatory response and reduced leptin, enhanced adiponectin expression in Pg-LPS treated adipocytes compared to adipocytes without Pg-LPS treatment (p<0.05). Pg-LPS induced apoptosis of adipocytes by enhancing XBP1 expression and modulating Bcl-2/Bax pathway associated molecules. In conclusion, Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) induces adipocytes injuries through modulating XBP1 expression and initialling mitochondria-mediated apoptosis.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"10 1","pages":"28-37"},"PeriodicalIF":3.3,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21623945.2020.1856527","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39125480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Reliability and variation in mitochondrial respiration in human adipose tissue. 人类脂肪组织中线粒体呼吸的可靠性和变异。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1991617

Ronni Eg Sahl, Eva Frederikke Høy Helms, Malte Schmücker, Mathias Flensted-Jensen, Arthur Ingersen, Thomas Morville, Flemming Dela, Jørn Wulff Helge, Steen Larsen

引用次数: 2

Low glucose and serum levels cause an increased inflammatory factor in 3T3-L1 cell through Akt, MAPKs and NF-кB activation. 低血糖和低血清水平通过激活Akt、MAPKs和NF-кB导致3T3-L1细胞炎症因子升高。

IF 3.3 4区生物学

Adipocyte Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1914420

Hirona Kugo, Wanida Sukketsiri, Kazuko Iwamoto, Satoki Suihara, Tatsuya Moriyama, Nobuhiro Zaima

{"title":"Low glucose and serum levels cause an increased inflammatory factor in 3T3-L1 cell through Akt, MAPKs and NF-кB activation.","authors":"Hirona Kugo, Wanida Sukketsiri, Kazuko Iwamoto, Satoki Suihara, Tatsuya Moriyama, Nobuhiro Zaima","doi":"10.1080/21623945.2021.1914420","DOIUrl":"https://doi.org/10.1080/21623945.2021.1914420","url":null,"abstract":"Abdominal aortic aneurysm (AAA) involves the degradation of vascular fibres, and dilation and rupture of the abdominal aorta. Hypoperfusion in the vascular walls due to stenosis of the vasa vasorum is reportedly a cause of AAA onset and involves the induction of adventitial ectopic adipocytes. Recent studies have reported that ectopic adipocytes are associated with AAA rupture in both human and hypoperfusion-induced animal models, highlighting the pathological importance of hypoperfusion and adipocytes in AAA. However, the relationship between hypoperfusion and AAA remains unknown. In this study, we investigated the changes in inflammation-related factors in adipocytes at low glucose and serum levels. Low glucose and serum levels enhanced the production of AAA-related factors in 3T3-L1 cells. Low glucose and serum levels increased the activation of protein kinase B (also known as Akt), extracellular signal-regulated protein kinase 1/2, p38, c-Jun N-terminal kinase, and nuclear factor (NF) кB at the protein level. The inflammatory factors and related signalling pathways were markedly decreased following the return of the cells to normal culture conditions. These data suggest that low glucose and serum levels increase the levels of inflammatory factors through the activation of Akt, mitogen activated protein kinase, and NF-κB signalling pathways.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"10 1","pages":"232-241"},"PeriodicalIF":3.3,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21623945.2021.1914420","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38826833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7