Adipocyte最新文献_第6页

IL-27 increases energy storage in white adipocytes by enhancing glucose uptake and fatty acid esterification. IL-27通过增强葡萄糖摄取和脂肪酸酯化来增加白色脂肪细胞的能量储存。

IF 3.3 4区生物学

Adipocyte Pub Date : 2023-12-01 Epub Date: 2023-11-10 DOI: 10.1080/21623945.2023.2276346

Chiara Scaffidi, Annie Srdic, Daniel Konrad, Stephan Wueest

{"title":"IL-27 increases energy storage in white adipocytes by enhancing glucose uptake and fatty acid esterification.","authors":"Chiara Scaffidi, Annie Srdic, Daniel Konrad, Stephan Wueest","doi":"10.1080/21623945.2023.2276346","DOIUrl":"10.1080/21623945.2023.2276346","url":null,"abstract":"The cytokine interleukin (IL)-27 has been reported to induce thermogenesis in white adipocytes. However, it remains unknown whether IL-27-mediated adipocyte energy dissipation is paralleled by an elevated energy supply from lipids and/or carbohydrates. We hypothesized that IL-27 increases lipolysis and glucose uptake in white adipocytes, thereby providing substrates for thermogenesis. Unexpectedly, we found that treatment of 3T3-L1 adipocytes with IL-27 reduced intra- and extracellular free fatty acid (FFA) concentrations and that phosphorylation of hormone-sensitive lipase (HSL) was not affected by IL-27. These results were confirmed in subcutaneous white adipocytes. Further, application of IL-27 to 3T3-L1 adipocytes increased intracellular triglyceride (TG) content but not mitochondrial ATP production nor expression of enzymes involved in beta-oxidation indicating that elevated esterification rather than oxidation causes FFA disappearance. In addition, IL-27 significantly increased GLUT1 protein levels, basal glucose uptake as well as glycolytic ATP production, suggesting that increased glycolytic flux due to IL-27 provides the glycerol backbone for TG synthesis. In conclusion, our findings suggest IL-27 increases glucose uptake and TG deposition in white adipocytes.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"12 1","pages":"2276346"},"PeriodicalIF":3.3,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10773535/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72208006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Matrix density regulates adipocyte phenotype. 基质密度调节脂肪细胞表型。

IF 3.3 4区生物学

Adipocyte Pub Date : 2023-12-01 Epub Date: 2023-10-10 DOI: 10.1080/21623945.2023.2268261

Alexander Ky, Atticus J McCoy, Carmen G Flesher, Nicole E Friend, Jie Li, Kore Akinleye, Christopher Patsalis, Carey N Lumeng, Andrew J Putnam, Robert W O'Rourke

引用次数: 0

Regulatory effects and mechanisms of exercise on activation of brown adipose tissue (BAT) and browning of white adipose tissue (WAT). 运动对棕色脂肪组织（BAT）活化和白色脂肪组织（WAT）褐变的调节作用及其机制。

IF 3.3 4区生物学

Adipocyte Pub Date : 2023-12-01 Epub Date: 2023-10-09 DOI: 10.1080/21623945.2023.2266147

Haijun Dong, Man Qin, Peng Wang, Shufan Li, Xing Wang

{"title":"Regulatory effects and mechanisms of exercise on activation of brown adipose tissue (BAT) and browning of white adipose tissue (WAT).","authors":"Haijun Dong, Man Qin, Peng Wang, Shufan Li, Xing Wang","doi":"10.1080/21623945.2023.2266147","DOIUrl":"10.1080/21623945.2023.2266147","url":null,"abstract":"Exercise is a universally acknowledged and healthy way to reducing body weight. However, the roles and mechanisms of exercise on metabolism of adipose tissue remain largely unclear. Adipose tissues include white adipose tissue (WAT), brown adipose tissue (BAT) and beige adipose tissue (BeAT). The main function of WAT is to store energy, while the BAT and BeAT can generate heat and consume energy. Therefore, promotion of BAT activation and WAT browning contributes to body weight loss. To date, many studies have suggested that exercise exerts the potential regulatory effects on BAT activation and WAT browning. In the present review, we compile the evidence for the regulatory effects of exercise on BAT activation and WAT browning and summarize the possible mechanisms whereby exercise modulates BAT activation and WAT browning, including activating sympathetic nervous system (SNS) and promoting the secretion of exerkines, with special focus on exerkines. These data might provide reference for prevention or treatment of obesity and the related metabolic disease through exercise.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":" ","pages":"2266147"},"PeriodicalIF":3.3,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/41/7d/KADI_12_2266147.PMC10563630.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41118749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes of insulin receptors in high fat and high glucose diet mice with insulin resistance. 胰岛素抵抗高脂高糖饮食小鼠胰岛素受体的变化。

IF 3.3 4区生物学

Adipocyte Pub Date : 2023-12-01 Epub Date: 2023-10-13 DOI: 10.1080/21623945.2023.2264444

Chen Lei, Jing Wang, Xin Li, Yuan-Yuan Mao, Jian-Qun Yan

{"title":"Changes of insulin receptors in high fat and high glucose diet mice with insulin resistance.","authors":"Chen Lei, Jing Wang, Xin Li, Yuan-Yuan Mao, Jian-Qun Yan","doi":"10.1080/21623945.2023.2264444","DOIUrl":"10.1080/21623945.2023.2264444","url":null,"abstract":"This study aimed to observe the expression of insulin-signaling molecules in different organs of mice with insulin resistance (IR). Firstly, mice were fed a high-fat and high-sugar diet (HF group) to establish an IR model, and the controls (NF group) were fed with a normal diet. Next, the weight, fasting blood glucose (FBG), serum insulin and insulin tolerance were detected. Pathological changes of liver tissues were observed by H&E staining. The expressions of INSR, IRS-1 and IRS-2 in the liver, skeletal muscle and ovary were measured by qRT-PCR and western blotting. As a result, compared with the NF group, the HF group mice had increased weight, FBG, insulin and IR index after 6-week of feeding as well as a worse performance in the insulin tolerance test and H&E staining showed fatty liver-like changes after 12-week of feeding, exhibited lower expression of INSR, IRS-1 and IRS-2 in the liver of mice at 6 and 12 weeks. The expression of INSR and IRS-1 in skeletal muscle tissues exhibited the same trend, while those in ovary organs showed the opposite trend. These results suggested that the insulin signaling alters in the liver, skeletal muscle and ovary organs with the progress of IR.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"12 1","pages":"2264444"},"PeriodicalIF":3.3,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10578188/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41187962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

In vitro studies of the renin-angiotensin system in human adipose tissue/adipocytes and possible relationship to SARS-CoV-2: a scoping review. 人脂肪组织/脂肪细胞肾素-血管紧张素系统的体外研究及其与SARS-CoV-2的可能关系:范围综述

IF 3.5 4区生物学

Adipocyte Pub Date : 2023-12-01 DOI: 10.1080/21623945.2023.2194034

Ryan Ting, Heidi Dutton, Alexander Sorisky

{"title":"In vitro studies of the renin-angiotensin system in human adipose tissue/adipocytes and possible relationship to SARS-CoV-2: a scoping review.","authors":"Ryan Ting, Heidi Dutton, Alexander Sorisky","doi":"10.1080/21623945.2023.2194034","DOIUrl":"10.1080/21623945.2023.2194034","url":null,"abstract":"The renin-angiotensin system (RAS) operates within adipose tissue. Obesity-related changes can affect adipose RAS, predisposing to hypertension, type 2 diabetes, and possibly severe COVID-19. We evaluated the in vitro research on human adipose RAS and identified gaps in the literature. Medline (Ovid), Embase (Ovid), Web of Science, Scopus, and 1findr were searched to identify relevant studies. Fifty primary studies met our inclusion criteria for analysis. Expression of RAS components (n = 14), role in differentiation (n = 14), association with inflammation (n = 15) or blood pressure (n = 7) were investigated. We found (1) obesity-related changes in RAS were frequently studied (30%); (2) an upswing of articles investigating adipose ACE-2 expression since the COVID-19 pandemic; (3) a paucity of papers on AT2R and Ang (1-7)/MasR which counterbalance Ang II/ART1; (4) weight loss lowered adipose ACE-2 mRNA expression; and (5) angiotensin receptor blockers (ARBs) reduced deleterious effects of angiotensin II. Overall, these studies link Ang II/ATR1 signalling to impaired adipogenesis and a pro-inflammatory dysfunctional adipose tissue, with ATR1 blockade limiting these responses. ACE-2 may mitigate Ang II effects by converting it to Ang(1-7) which binds MasR. More work is needed to understand adipose RAS in various pathologic states such as obesity and COVID-19 infection.T.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"12 1","pages":"2194034"},"PeriodicalIF":3.5,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10054178/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9286679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioactive peptides PDBSN improve mitochondrial function and suppression the oxidative stress in human adiposity cells. 生物活性肽PDBSN改善人类脂肪细胞的线粒体功能并抑制氧化应激。

IF 3.3 4区生物学

Adipocyte Pub Date : 2023-11-09 DOI: 10.1080/21623945.2023.2278213

Huiping Shen, Yong Lei, Wen Xie, Tieliang Ma, Li Bao, Qin Gao, Bingyu Chen, Biao Dai, Dani Qin

{"title":"Bioactive peptides PDBSN improve mitochondrial function and suppression the oxidative stress in human adiposity cells.","authors":"Huiping Shen, Yong Lei, Wen Xie, Tieliang Ma, Li Bao, Qin Gao, Bingyu Chen, Biao Dai, Dani Qin","doi":"10.1080/21623945.2023.2278213","DOIUrl":"10.1080/21623945.2023.2278213","url":null,"abstract":"Introduction: Mitochondria are essential for generating cellular energy and are significant in the pathogenesis of obesity. Peptide PDBSN has been demonstrated to inhibit the adipogenic differentiation of adipocytes in vitro and improves metabolic homoeostasis in vivo. Therefore, in this study, we further investigated the effects of PDBSN on the morphology, synthesis, and function of adipocyte mitochondria. Methods: Human visceral and subcutaneous primary preadipocytes (HPA-v and HPA-s) were cultured into mature adipocytes. Intracellular triglyceride content was assessed using oil-red O staining and tissue triglyceride determination. Gene and protein levels associated with mitochondrial synthesis were detected using real-time quantitative polymerase chain reaction and western blotting. Mitochondrial membrane potentials and ROS were detected using fluorescent indicators. Morphological changes were observed by electron microscopy. Results: PDBSN significantly increased mitochondrial membrane potential (MMP), while decreasing intracellular triglyceride (TG) and intracellular reactive oxygen species (ROS) levels. On the other hand, the transcription and protein levels of genetic marker genes PGC1-α and MTFA were significantly up-regulated after PDBSN administration. Further studies showed that transcriptional and protein levels of mitochondrial fusion and fission genetic markers MFN1, MFN2, NRF1, and DRP1 increased. Conclusion: PDBSN significantly reduces intracellular TG and ROS levels and increases MMP. The maximum respiratory capacity in adults significantly increases after PDBSN administration, and ROS levels are significantly reduced. This suggests that PDBSN improves mitochondrial function to some extent, which not only provides an essential basis for the pathophysiology of obesity but also provides insights for the development of new drugs to treat obesity and metabolic diseases.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":" ","pages":"2278213"},"PeriodicalIF":3.3,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71520168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lysosomal acid lipase promotes endothelial proliferation in cold-activated adipose tissue. 溶酶体酸性脂肪酶促进冷活化脂肪组织内皮细胞增殖。

IF 3.3 4区生物学

Adipocyte Pub Date : 2022-12-01 DOI: 10.1080/21623945.2021.2013416

Alexander W Fischer, Michelle Y Jaeckstein, Joerg Heeren

{"title":"Lysosomal acid lipase promotes endothelial proliferation in cold-activated adipose tissue.","authors":"Alexander W Fischer, Michelle Y Jaeckstein, Joerg Heeren","doi":"10.1080/21623945.2021.2013416","DOIUrl":"https://doi.org/10.1080/21623945.2021.2013416","url":null,"abstract":"Oxidative tissues such as brown adipose tissue and muscle internalize large amounts of circulating lipids and glucose as energy source. Endothelial cells (ECs) provide a platform for regulated transport and processing of blood-borne nutrients. Next to this role, it has become recognized that intercellular crosstalk between ECs and underlying parenchymal cells is indispensable for maintenance of tissue homoeostasis. Here, we comment on our recent observation that capillary ECs in thermogenic adipose tissues take up and metabolize entire triglyceride-rich lipoprotein (TRL) particles in response to cold exposure. This process is dependent on CD36, lipoprotein lipase (LPL) and lysosomal acid lipase (LAL). Remarkably, loss of LAL specifically in endothelial cells results in impaired endothelial proliferation and diminished thermogenic adaptation. Mechanistically, cell culture experiments indicate that LAL-mediated TRL processing leads to the generation of reactive oxygen species, which in turn activate hypoxia-induced factor (HIF)-mediated proliferative responses. In the current manuscript, we provide in vivo evidence that LAL-deficiency impairs proliferation of endothelial cells in thermogenic adipose tissue. In addition, we show uptake of nanoparticle-labelled TRL and LAL expression in cardiac endothelial cells, suggesting a physiological function of endothelial lipoprotein processing not only in thermogenic adipose tissue but also in cardiac muscle.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":" ","pages":"28-33"},"PeriodicalIF":3.3,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8726628/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39764606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Brown adipose tissue influences adiponectin and thyroid hormone changes during Graves' disease therapy. 褐色脂肪组织影响格雷夫斯病治疗期间脂联素和甲状腺激素的变化。

IF 3.3 4区生物学

Adipocyte Pub Date : 2022-12-01 DOI: 10.1080/21623945.2022.2104509

Wei-En Ho, Lijuan Sun, Hui Jen Goh, Mya Thway Tint, Lei Sun, Melvin Khee Shing Leow

{"title":"Brown adipose tissue influences adiponectin and thyroid hormone changes during Graves' disease therapy.","authors":"Wei-En Ho, Lijuan Sun, Hui Jen Goh, Mya Thway Tint, Lei Sun, Melvin Khee Shing Leow","doi":"10.1080/21623945.2022.2104509","DOIUrl":"https://doi.org/10.1080/21623945.2022.2104509","url":null,"abstract":"Thyroid hormones (TH), adiponectin and brown adipose tissue (BAT) are regulators of energy homoeostasis. Influence of BAT activity on the relationship between TH and adiponectin remains unexplored. The aim of the study was to identify the relationship between TH and adiponectin and to clarify the impact of active BAT on the metabolic effects of adiponectin before and after the correction of thyrotoxicosis. Twenty-one patients with newly diagnosed hyperthyroidism from Graves' disease were recruited. A titration dosing regimen of thionamide anti-thyroid drug (ATD) was used to establish euthyroidism over 12-24 weeks. Anthropometric, biochemical and adipocytokine parameters were measured before and after control of hyperthyroidism. BAT activity was quantified by fusion 18 F-fluorodeoxyglucose (18 F-FDG) PET/MR imaging, and patients were grouped based on BAT status. Plasma adiponectin level was significantly increased following correction of hyperthyroidism in the overall sample. Free thyroxine (FT4) was also identified as a predictor of adiponectin level in thyroid dysfunction. However, significant changes in adiponectin level and correlations involving adiponectin were absent in BAT-positive patients but maintained in BAT-negative patients. BAT activity diminishes the correlative relationship with body composition and abolishes TH and adiponectin relationships when transitioning from a hyperthyroid to euthyroid state.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":" ","pages":"389-400"},"PeriodicalIF":3.3,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9336474/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40565755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The mechanisms underlying olanzapine-induced insulin resistance via the brown adipose tissue and the therapy in rats. 奥氮平通过棕色脂肪组织诱导大鼠胰岛素抵抗的机制及治疗。

IF 3.3 4区生物学

Adipocyte Pub Date : 2022-12-01 DOI: 10.1080/21623945.2022.2026590

Jing Wang, Qian Wu, Yuan Zhou, Liangyu Yu, Lixiu Yu, Yahui Deng, Chuyue Tu, Weiyong Li

{"title":"The mechanisms underlying olanzapine-induced insulin resistance via the brown adipose tissue and the therapy in rats.","authors":"Jing Wang, Qian Wu, Yuan Zhou, Liangyu Yu, Lixiu Yu, Yahui Deng, Chuyue Tu, Weiyong Li","doi":"10.1080/21623945.2022.2026590","DOIUrl":"https://doi.org/10.1080/21623945.2022.2026590","url":null,"abstract":"A rapid increase has been observed in insulin resistance (IR) incidence induced by a long-term olanzapine treatment with no better ways to avoid it. Our study aimed to demonstrate the mechanism underlying the olanzapine-induced insulin resistance and find appropriate drug interventions. In this study, firstly, we constructed rat insulin resistance model using a two-month gavage of olanzapine and used the main active ingredient mixture of Gegen Qinlian Decoction for the treatment. The activity of brown adipose tissue (BAT) was measured using the PET/CT scan, whereas Western blot and quantitative real-time PCR were used to detect the expression of GLUT4 and UCP1. The results showed that the long-term administration of olanzapine impaired glucose tolerance and produced insulin resistance in rats, while Gegen Qinlian Decoction could improve this side effect. The results of the PET/CT scan showed that the BAT activity in the insulin-resistant rats was significantly lower than that of the Gegen Qinlian Decoction treated rats. Also, the expression of GLUT4 and UCP1 in the insulin resistance group showed a significant decrease, which could be up-regulated by Gegen Qinliane Decoction treatment. The results of both in vivo and in vitro experiments were consistent. we demonstrated that the olanzapine could induce IR in vitro and in vivo by decreasing the expression of UCP1; thus, suppressing the thermogenesis of BAT and impairing glucose uptake. More importantly, we demonstrated a possible novel strategy to improve the olanzapine-induced IR by Gegen Qinlian Decoction.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":" ","pages":"84-98"},"PeriodicalIF":3.3,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8786323/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39850842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adipose-derived mesenchymal stem cell-secreted extracellular vesicles alleviate non-alcoholic fatty liver disease via delivering miR-223-3p. 脂肪源性间充质干细胞分泌的细胞外囊泡通过传递miR-223-3p缓解非酒精性脂肪肝疾病。

IF 3.3 4区生物学

Adipocyte Pub Date : 2022-12-01 DOI: 10.1080/21623945.2022.2098583

Qinghui Niu, Ting Wang, Zhiqiang Wang, Feng Wang, Deyu Huang, Huali Sun, Hanyun Liu

{"title":"Adipose-derived mesenchymal stem cell-secreted extracellular vesicles alleviate non-alcoholic fatty liver disease via delivering miR-223-3p.","authors":"Qinghui Niu, Ting Wang, Zhiqiang Wang, Feng Wang, Deyu Huang, Huali Sun, Hanyun Liu","doi":"10.1080/21623945.2022.2098583","DOIUrl":"https://doi.org/10.1080/21623945.2022.2098583","url":null,"abstract":"Increasing studies have identified the potential of mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) in non-alcoholic fatty liver disease (NAFLD) treatment. Hence, we further focused on the potential of adipose-derived MSC (ADSC)-EVs in NAFLD by delivering miR-223-3p. The uptake of isolated ADSC-EVs by hepatocytes was assessed, and the expression of miR-223-3p in ADSC-EVs and hepatocytes was characterized. It was established that miR-223-3p, enriched in ADSC-EVs, could be delivered by ADSC-EVs into hepatocytes. Using co-culture system and gain-of-function approach, we evaluated the effect of ADSC-EVs carrying miR-223-3p on lipid accumulation and liver fibrosis in pyrrolizidine alkaloids (PA)-induced hepatocytes and a high-fat diet-induced NAFLD mouse model. Bioinformatics websites and dual-luciferase reporter gene assay were performed to determine the interactions between miR-223-3p and E2F1, which was further validated by rescue experiments. ADSC-EVs containing miR-223-3p displayed suppressive effects on lipid accumulation and liver fibrosis through E2F1 inhibition, since E2F1 was demonstrated as a target gene of miR-223-3p. The protective role of ADSC-EVs by delivering miR-223-3p was then confirmed in the mouse model. Collectively, this study elucidated that ADSC-EVs delayed the progression NAFLD through the delivery of anti-fibrotic miR-223-3p and subsequent E2F1 suppression, which may suggest miR-223-3p-loaded ADSC-EVs to be a potential therapeutic approach for NAFLD.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"11 1","pages":"572-587"},"PeriodicalIF":3.3,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9481107/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10626499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12