Adipocyte最新文献_第5页

Defining the role of 2,2',4,4'-tetrabromodiphenyl ether in 3T3-L1 cellular differentiation by transcriptome sequencing analysis. 通过转录组测序分析确定2,2',4,4'-四溴联苯醚在3T3-L1细胞分化中的作用。

IF 3.5 4区生物学

Adipocyte Pub Date : 2024-12-01 Epub Date: 2024-12-07 DOI: 10.1080/21623945.2024.2430717

Zao-Ling Liu, Aerna Qiayimaerdan, Yong Fan, Shu-Rui Jiang, Zunire Tuerxuna, Meng-Lin Wang, Haiqiemuhan Abudureheman

{"title":"Defining the role of 2,2',4,4'-tetrabromodiphenyl ether in 3T3-L1 cellular differentiation by transcriptome sequencing analysis.","authors":"Zao-Ling Liu, Aerna Qiayimaerdan, Yong Fan, Shu-Rui Jiang, Zunire Tuerxuna, Meng-Lin Wang, Haiqiemuhan Abudureheman","doi":"10.1080/21623945.2024.2430717","DOIUrl":"10.1080/21623945.2024.2430717","url":null,"abstract":"This study aims to investigates the effect of 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) on the differentiation of 3T3-L1 cells and its mechanism of action. These 3T3-L1 cells were induced to differentiate in vitro using methylisobutylxanthine, dexamethasone, and insulin conditions, then exposed to either 1% DMSO as a control group or varying concentrations of BDE-47 (2.5 μM, 7.5 μM, 12.5 μM, 18.75 μM, and 25 μM). Oil red O staining showed that the absorbance value of the BDE-47 exposure groups was higher than that of the control group (p < 0.05). This study identified 722 common genes between the differentially expressed genes of each exposure group. Using Cytoscape 10 hub genes were identified as Actb, Cdk1, Myc, Ccnb1, Aurkb, Plk1, Aurka, Pparg, Kif11, and Casp3. Enrichment analysis data revealed that the effects of BDE-47 on 3T3-L1 cell differentiation were associated with the cell cycle, p53 signalling, and PPARγ pathways. The transcription factor genes, KAT2A, MAX, SIN3A, TBP, and EP300, were shown to be associated with the PPARγ pathway. The mRNA expression of PPARγ in each exposure group was higher than that in the control group (p < 0.05), and a bimodal distribution between PPARγ mRNA expression and BDE-47 dose was observed. These findings indicate that BDE-47 May activate the PPARγ pathway and mitotic pathway to regulate the cell cycle and induce adipocyte differentiation.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"13 1","pages":"2430717"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11633175/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142790888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research progress on the pathogenesis of multiple symmetrical lipomatosis. 多发性对称性脂肪瘤病发病机制的研究进展。

IF 3.5 4区生物学

Adipocyte Pub Date : 2024-12-01 Epub Date: 2024-10-17 DOI: 10.1080/21623945.2024.2416681

Bo Hu, Ze Wang, Tengxiao Ma, Pengfei Fan, Lei Li

引用次数: 0

Anaesthetics reduce the viability of adipose-derived stem cells. 麻醉剂会降低脂肪干细胞的活力。

IF 3.5 4区生物学

Adipocyte Pub Date : 2024-12-01 Epub Date: 2024-05-23 DOI: 10.1080/21623945.2024.2351870

Maria Bugajska-Liedtke, Nadia Fatyga, Aleksander Brzozowski, Anna Bajek, Małgorzata Maj

{"title":"Anaesthetics reduce the viability of adipose-derived stem cells.","authors":"Maria Bugajska-Liedtke, Nadia Fatyga, Aleksander Brzozowski, Anna Bajek, Małgorzata Maj","doi":"10.1080/21623945.2024.2351870","DOIUrl":"10.1080/21623945.2024.2351870","url":null,"abstract":"Adipose-derived stem cells (ADSCs) are characterized by their low immunogenicity and unique immunosuppressive properties, providing many opportunities for autologous transplantation in regenerative medicine and plastic surgery. These methods are characterized by low rejection rates and intense stimulation of tissue regeneration. However, procedures during which fat tissue is harvested occur under local anaesthesia. To better understand the effects and mechanisms of anaesthetic compounds in cosmetic and therapeutic procedures, the present study used a mixture of these compounds (0.1% epinephrine, 8.4% sodium bicarbonate, and 4% articaine) and examined their impact on a human adipose-derived stem cell line. The results showed anesthetics' negative, dose-dependent effect on cell viability and proliferation, especially during the first 24 h of incubation. After extending the exposure to 48 and 72 h of incubation, cells adapted to new culture conditions. In contrast, no significant changes were observed in immunophenotype, cell cycle progression, and apoptosis. The results obtained from this study provide information on the effect of the selected mixture of anaesthetics on the characteristics and function of ASC52telo cells. The undesirable changes in the metabolic activity of cells suggest the need to search for new drugs to harvest cells with unaltered properties and higher efficacy in aesthetic medicine treatments.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"13 1","pages":"2351870"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11123512/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141080272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and analysis of scaffold-free adipose spheroids. 开发和分析无支架脂肪球。

IF 3.5 4区生物学

Adipocyte Pub Date : 2024-12-01 Epub Date: 2024-06-12 DOI: 10.1080/21623945.2024.2347215

Jesse Liszewski, Aloysious Klingelhutz, Edward A Sander, James Ankrum

{"title":"Development and analysis of scaffold-free adipose spheroids.","authors":"Jesse Liszewski, Aloysious Klingelhutz, Edward A Sander, James Ankrum","doi":"10.1080/21623945.2024.2347215","DOIUrl":"10.1080/21623945.2024.2347215","url":null,"abstract":"Adipose tissue plays a crucial role in metabolic syndrome, autoimmune diseases, and many cancers. Because of adipose's role in so many aspects of human health, there is a critical need for in vitro models that replicate adipose architecture and function. Traditional monolayer models, despite their convenience, are limited, showing heterogeneity and functional differences compared to 3D models. While monolayer cultures struggle with detachment and inefficient differentiation, healthy adipocytes in 3D culture accumulate large lipid droplets, secrete adiponectin, and produce low levels of inflammatory cytokines. The shift from monolayer models to more complex 3D models aims to better replicate the physiology of healthy adipose tissue in culture. This study introduces a simple and accessible protocol for generating adipose organoids using a scaffold-free spheroid model. The method, utilizing either 96-well spheroid plates or agarose micromolds, demonstrates increased throughput, uniformity, and ease of handling compared to previous techniques. This protocol allows for diverse applications, including drug testing, toxin screening, tissue engineering, and co-culturing. The choice between the two methods depends on the experimental goals, with the 96-well plate providing individualized control and the micromold offering scale advantages. The outlined protocol covers isolation, expansion, and characterization of stromal vascular fraction cells, followed by detailed steps for spheroid formation and optional downstream analyses.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"13 1","pages":"2347215"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11174133/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141305076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leptin secreted by adipocytes promotes EMT transition and endometrial cancer progression via the JAK2/STAT3 signalling pathway 脂肪细胞分泌的瘦素通过 JAK2/STAT3 信号通路促进 EMT 转变和子宫内膜癌的进展

IF 3.3 4区生物学

Adipocyte Pub Date : 2023-12-13 DOI: 10.1080/21623945.2023.2293273

Lifan Shen, Chen Zhang, Kaiying Cui, Xin Liang, Genhai Zhu, Lan Hong

引用次数: 0

Inhibition of AHCY impedes proliferation and differentiation of mouse and human adipocyte progenitor cells 抑制 AHCY 会阻碍小鼠和人类脂肪细胞祖细胞的增殖和分化

IF 3.3 4区生物学

Adipocyte Pub Date : 2023-12-08 DOI: 10.1080/21623945.2023.2290218

Paula Boczki, Marco Colombo, Juliane Weiner, Inka Rapöhn, Martin Lacher, Wieland Kiess, Martha Hanschkow, Antje Körner, Kathrin Landgraf

引用次数: 0

An optimised protocol for the investigation of insulin signalling in a human cell culture model of adipogenesis. 胰岛素信号在脂肪生成的人类细胞培养模型中研究的优化方案。

IF 3.5 4区生物学

Adipocyte Pub Date : 2023-12-01 DOI: 10.1080/21623945.2023.2179339

Jonathan M Gamwell, Keanu Paphiti, Leanne Hodson, Fredrik Karpe, Katherine E Pinnick, Marijana Todorčević

{"title":"An optimised protocol for the investigation of insulin signalling in a human cell culture model of adipogenesis.","authors":"Jonathan M Gamwell, Keanu Paphiti, Leanne Hodson, Fredrik Karpe, Katherine E Pinnick, Marijana Todorčević","doi":"10.1080/21623945.2023.2179339","DOIUrl":"10.1080/21623945.2023.2179339","url":null,"abstract":"While there is no standardized protocol for the differentiation of human adipocytes in culture, common themes exist in the use of supra-physiological glucose and hormone concentrations, and an absence of exogenous fatty acids. These factors can have detrimental effects on some aspects of adipogenesis and adipocyte function. Here, we present methods for modifying the adipogenic differentiation protocol to overcome impaired glucose uptake and insulin signalling in human adipose-derived stem cell lines derived from the stromal vascular fraction of abdominal and gluteal subcutaneous adipose tissue. By reducing the length of exposure to adipogenic hormones, in combination with a physiological glucose concentration (5 mM), and the provision of exogenous fatty acids (reflecting typical dietary fatty acids), we were able to restore early insulin signalling events and glucose uptake, which were impaired by extended use of hormones and a high glucose concentration, respectively. Furthermore, the addition of exogenous fatty acids greatly increased the storage of triglycerides and removed the artificial demand to synthesize all fatty acids by de novo lipogenesis. Thus, modifying the adipogenic cocktail can enhance functional aspects of human adipocytes in vitro and is an important variable to consider prior to in vitro investigations into adipocyte biology.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"12 1","pages":"2179339"},"PeriodicalIF":3.5,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9980465/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9463287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An optimized method for Oil Red O staining with the salicylic acid ethanol solution. 水杨酸乙醇溶液对油红O染色的优化方法。

IF 3.5 4区生物学

Adipocyte Pub Date : 2023-12-01 DOI: 10.1080/21623945.2023.2179334

Junbao Du, Li Zhao, Quan Kang, Yun He, Yang Bi

引用次数: 0

Integrative bioinformatics analysis to screen key genes and signalling pathways related to ferroptosis in obesity. 综合生物信息学分析，筛选与肥胖脱铁症相关的关键基因和信号通路。

IF 3.3 4区生物学

Adipocyte Pub Date : 2023-12-01 Epub Date: 2023-10-25 DOI: 10.1080/21623945.2023.2264442

Ming-Ke Li, Chang Xing, Lan-Qing Ma

{"title":"Integrative bioinformatics analysis to screen key genes and signalling pathways related to ferroptosis in obesity.","authors":"Ming-Ke Li, Chang Xing, Lan-Qing Ma","doi":"10.1080/21623945.2023.2264442","DOIUrl":"10.1080/21623945.2023.2264442","url":null,"abstract":"Ferroptosis is closely associated with the development of disease in the body. However, there are few studies on ferroptosis-related genes (FRGs) in obesity. Therefore, key genes and signalling pathways related to ferroptosis in obesity were screened. Briefly, the RNA sequencing data of obesity and the non-obesity human samples and 259 FRGs were downloaded from GEO database and FerrDb database, respectively. The obesity-related module genes were firstly screened by weighted gene co-expression network analysis (WGCNA) and crossed with differentially expressed genes (DEGs) of obesity/normal samples and FRGs to obtain obesity-ferroptosis related (OFR) DEGs. Then, key genes were screened by PPI network. Next, the correlation of key genes and differential immune cells between obesity and normal samples were further explored by immune infiltration analysis. Finally, microRNA (miRNA)-messenger RNA (mRNA), transcription factor (TF)-mRNA networks and drug-gene interaction networks were constructed. As a result, 17 OFR DEGs were obtained, which mainly participated in processes such as lipid metabolism or adipocyte differentiation. The 4 key genes, STAT3, IL-6, PTGS2, and VEGFA, constituted the network. M2 macrophages, T cells CD8, mast cells activated, and T cells CD4 memory resting had significant differences between obesity and normal samples. Moreover, 51 miRNAs and 164 drugs were predicted for 4 key genes. All in all, this study has screened 4 FRGs, including IL-6, VEGFA, STAT3, and PTGS2, in obesity patients.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"12 1","pages":"2264442"},"PeriodicalIF":3.3,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ba/1f/KADI_12_2264442.PMC10601513.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50160338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IL-27 increases energy storage in white adipocytes by enhancing glucose uptake and fatty acid esterification. IL-27通过增强葡萄糖摄取和脂肪酸酯化来增加白色脂肪细胞的能量储存。

IF 3.3 4区生物学

Adipocyte Pub Date : 2023-12-01 Epub Date: 2023-11-10 DOI: 10.1080/21623945.2023.2276346

Chiara Scaffidi, Annie Srdic, Daniel Konrad, Stephan Wueest

{"title":"IL-27 increases energy storage in white adipocytes by enhancing glucose uptake and fatty acid esterification.","authors":"Chiara Scaffidi, Annie Srdic, Daniel Konrad, Stephan Wueest","doi":"10.1080/21623945.2023.2276346","DOIUrl":"10.1080/21623945.2023.2276346","url":null,"abstract":"The cytokine interleukin (IL)-27 has been reported to induce thermogenesis in white adipocytes. However, it remains unknown whether IL-27-mediated adipocyte energy dissipation is paralleled by an elevated energy supply from lipids and/or carbohydrates. We hypothesized that IL-27 increases lipolysis and glucose uptake in white adipocytes, thereby providing substrates for thermogenesis. Unexpectedly, we found that treatment of 3T3-L1 adipocytes with IL-27 reduced intra- and extracellular free fatty acid (FFA) concentrations and that phosphorylation of hormone-sensitive lipase (HSL) was not affected by IL-27. These results were confirmed in subcutaneous white adipocytes. Further, application of IL-27 to 3T3-L1 adipocytes increased intracellular triglyceride (TG) content but not mitochondrial ATP production nor expression of enzymes involved in beta-oxidation indicating that elevated esterification rather than oxidation causes FFA disappearance. In addition, IL-27 significantly increased GLUT1 protein levels, basal glucose uptake as well as glycolytic ATP production, suggesting that increased glycolytic flux due to IL-27 provides the glycerol backbone for TG synthesis. In conclusion, our findings suggest IL-27 increases glucose uptake and TG deposition in white adipocytes.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"12 1","pages":"2276346"},"PeriodicalIF":3.3,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10773535/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72208006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0