Adipocyte最新文献_第5页

WDR4-mediate tRNA m7G modification to promote mitophagy and browning of white adipose tissue for ameliorating obesity in male mice. wdr4介导tRNA m7G修饰促进白色脂肪组织的线粒体自噬和褐化以改善雄性小鼠肥胖

IF 3.1 4区生物学

Adipocyte Pub Date : 2025-12-01 Epub Date: 2025-11-25 DOI: 10.1080/21623945.2025.2588888

Beisi Lin, Chaofan Wang, Yanling Yang, Zhigu Liu, Panwei Mu, Wen Xu, Yonghui Li

{"title":"WDR4-mediate tRNA m7G modification to promote mitophagy and browning of white adipose tissue for ameliorating obesity in male mice.","authors":"Beisi Lin, Chaofan Wang, Yanling Yang, Zhigu Liu, Panwei Mu, Wen Xu, Yonghui Li","doi":"10.1080/21623945.2025.2588888","DOIUrl":"10.1080/21623945.2025.2588888","url":null,"abstract":"Objective: Brown adipose tissue activation is a potential anti-obesity strategy. N7-methylguanosine (m7G) modification is a novel RNA epigenetic modification, but its role in adipose metabolism remains unexplored.Methods: Male mice were fed a high-fat diet (HFD), followed by PCR array screening. Gain-of-function experiments and TRAC-seq were employed to explore WDR4 function.Result: A Mouse Epigenetic Modification Enzymes PCR Array revealed that WDR4 expression showed the most pronounced downregulation in HFD mice. Overexpression of WDR4 in 3T3-L1 cells and primary adipocytes significantly increased UCP1 expression and suppressed lipid droplet formation, and enhanced mitophagy as evidenced by mitochondrial ultrastructure, autophagic vesicles, and LC3 expression. Suppression of mitophagy using 3-MA and bafilomycin A1 attenuated WDR4-induced adipocyte browning. WDR4 overexpression enhanced translational activity and reshaped the tRNA m7G methylome in 3T3-L1 adipocytes, specifically induced 38 unique tRNA m7G modification sites, and increasing cleavage scores of multiple tRNAs. GSE229240 dataset revealed that WDR4 mutation significantly reduced translation efficiency of 195 genes enriched in the TGF-β signalling , including BMP8B. Knockdown of BMP8B partially counteracted WDR4-mediated mitophagy.Conclusion: WDR4 promotes adipocyte browning by enhancing BMP8B translation through tRNA m7G modification, revealing a novel m7G epitranscriptomic mechanism with therapeutic potential for obesity.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"14 1","pages":"2588888"},"PeriodicalIF":3.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12667666/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145601683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fatty acid composition of lipid fractions in white- and brown-like adipocytes derived from human mesenchymal stem cells. 来源于人间充质干细胞的白色和棕色样脂肪细胞中脂质组分的脂肪酸组成。

IF 3.1 4区生物学

Adipocyte Pub Date : 2025-12-01 Epub Date: 2025-10-01 DOI: 10.1080/21623945.2025.2566481

Khadijeh Abbasi, Amir Mehdizadeh, Hamed Hamishehkar, Mohammad Nouri, Masoud Darabi

{"title":"Fatty acid composition of lipid fractions in white- and brown-like adipocytes derived from human mesenchymal stem cells.","authors":"Khadijeh Abbasi, Amir Mehdizadeh, Hamed Hamishehkar, Mohammad Nouri, Masoud Darabi","doi":"10.1080/21623945.2025.2566481","DOIUrl":"10.1080/21623945.2025.2566481","url":null,"abstract":"White and brown adipocytes differ markedly in lipid composition and metabolic function. White adipocytes primarily serve as energy storage depots, whereas brown adipocytes are mitochondria-rich and specialized for thermogenesis. However, the lipidomic profiles of white-like (WLAs) and brown-like adipocytes (BLAs) differentiated from human mesenchymal stem cells (MSCs) remain incompletely characterized. Human adipose-derived MSCs were differentiated into WLAs and BLAs. Lipid fractions were isolated and analysed by gas-liquid chromatography. Fatty acid composition data were used to calculate indices of stearoyl-CoA desaturase-1 (SCD1) activity, elongation, and ω6 synthesis. Compared to MSCs, BLAs showed consistently elevated oleate (≥4.2-fold) and stearate (≥2.3-fold), along with reduced palmitate (≤-20%) and linoleate (≤-28%) across phospholipid, triglyceride, and free fatty acid fractions. WLAs versus MSCs showed similar trends, with oleate increasing up to 15-fold and palmitate decreasing by 67-82% depending on the lipid class. SCD1 activity and elongation indices were elevated in WLAs (SCD1: up to 4.7-fold; elongation: up to 28-fold). The ω6 synthesis index was also increased in triglyceride and free fatty acid fractions of WLAs (≥3.3-fold), but markedly suppressed in BLAs (≤-88.7%). WLAs and BLAs differentiated from MSCs exhibit distinct lipid profiles and inferred enzymatic activity patterns, reflecting their respective capacities for lipid storage and metabolic flexibility. These findings provide a foundation for future translational research aimed at targeting adipose tissue in obesity and metabolic diseases.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"14 1","pages":"2566481"},"PeriodicalIF":3.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12498536/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Crosstalk between microRNAs and autophagy in adipocyte differentiation: emerging therapeutic targets for obesity. 脂肪细胞分化中microrna与自噬之间的串扰：肥胖症的新治疗靶点。

IF 3.1 4区生物学

Adipocyte Pub Date : 2025-12-01 Epub Date: 2025-10-27 DOI: 10.1080/21623945.2025.2580741

Shan Gao

引用次数: 0

Disrupted adipokine secretion and inflammatory responses in human adipocyte hypertrophy. 人脂肪细胞肥大中脂肪因子分泌中断和炎症反应。

IF 3.1 4区生物学

Adipocyte Pub Date : 2025-12-01 Epub Date: 2025-04-03 DOI: 10.1080/21623945.2025.2485927

Dan Gao, Chen Bing, Helen R Griffiths

引用次数: 0

Hematopoietic stem cell-derived adipocytes suppress leptin production, and attenuate ovariectomy-induced inhibition of physical activity and insulin sensitivity in female mice. 造血干细胞来源的脂肪细胞抑制瘦素的产生，并减弱卵巢切除引起的雌性小鼠身体活动和胰岛素敏感性的抑制。

IF 3.3 4区生物学

Adipocyte Pub Date : 2025-12-01 Epub Date: 2025-08-04 DOI: 10.1080/21623945.2025.2536813

Andrew E Libby, Timothy M Sullivan, Joanne K Maltzahn, Matthew R Jackman, Kathleen M Gavin, Paul S MacLean, Wendy M Kohrt, Susan M Majka, Dwight J Klemm

{"title":"Hematopoietic stem cell-derived adipocytes suppress leptin production, and attenuate ovariectomy-induced inhibition of physical activity and insulin sensitivity in female mice.","authors":"Andrew E Libby, Timothy M Sullivan, Joanne K Maltzahn, Matthew R Jackman, Kathleen M Gavin, Paul S MacLean, Wendy M Kohrt, Susan M Majka, Dwight J Klemm","doi":"10.1080/21623945.2025.2536813","DOIUrl":"10.1080/21623945.2025.2536813","url":null,"abstract":"A subpopulation of adipocytes in mice and humans is produced from haematopoietic stem cells rather than mesenchymal progenitors; the source of conventional white and brown/beige adipocytes. The abundance of these haematopoietic stem cell-derived adipocytes (HSCDAs) is elevated in female mice by ovariectomy (OVX) or oestrogen receptor alpha (ERα) knockdown, suggesting that they may be involved in the metabolic and inflammatory pathology that accompany the loss of oestrogen signalling. However, we previously demonstrated that ablation of HSCDAs elevated circulating leptin levels while suppressing physical activity and insulin sensitivity. Here, we tested the combined impact of OVX with and without HSCDA ablation. We discovered that HSCDA depletion plus OVX raised circulating leptin levels more than HSCDA depletion alone. Likewise, while HSCDA depletion or OVX alone inhibited physical activity and insulin responsiveness, their combination further suppressed these endpoints. Other physiologic endpoints were regulated by OVX alone. We conclude that HSCDAs play a role inthe maintenance of a subset of metabolic endpoints related to normal adipose tissue function, and their elevated production in models of female sex hormone suppression occurs to normalize these endpoints. The results highlight the ability of HSCDAs to target physical activity and insulin responsiveness, possibly by normalizing leptin production.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"14 1","pages":"2536813"},"PeriodicalIF":3.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12323437/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144783225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adipose tissue and adipose-derived stromal cells can reduce skin contraction in an in vitro tissue engineered full thickness skin model. 在体外组织工程全层皮肤模型中，脂肪组织和脂肪来源的基质细胞可以减少皮肤收缩。

IF 3.1 4区生物学

Adipocyte Pub Date : 2025-12-01 Epub Date: 2025-03-19 DOI: 10.1080/21623945.2025.2473367

Victoria L Workman, Anna-Victoria Giblin, Nicola H Green, Sheila MacNeil, Vanessa Hearnden

{"title":"Adipose tissue and adipose-derived stromal cells can reduce skin contraction in an in vitro tissue engineered full thickness skin model.","authors":"Victoria L Workman, Anna-Victoria Giblin, Nicola H Green, Sheila MacNeil, Vanessa Hearnden","doi":"10.1080/21623945.2025.2473367","DOIUrl":"10.1080/21623945.2025.2473367","url":null,"abstract":"Skin contracts during wound healing to facilitate wound closure. In some patients, skin contraction can lead to the formation of skin contractures that limit movement, impair function, and significantly impact well-being. Current treatment options for skin contractures are burdensome for patients, and there is a high risk of recurrence. Autologous fat grafting can improve the structure and function of scarred skin; however, relatively little is known about the effect of fat on skin contraction. In this study, an in vitro tissue-engineered model of human skin was used to test the effects of adipose tissue and adipose-derived stromal cells on skin contraction. Untreated tissue-engineered skin contracted to approximately 60% of the original area over 14 days in culture. The addition of adipose tissue reduced this contraction by 50%. Adipose tissue, which was emulsified or concentrated and high doses of adipose-derived stromal cells (ADSC) were able to inhibit contraction to a similar degree; however, lower doses of ADSC did not show the same effect. In conclusion, the subcutaneous application of adipose tissue has the potential to inhibit skin contraction. This study provides in vitro evidence to support the use of autologous fat grafting to prevent skin contraction in patients most at risk.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"14 1","pages":"2473367"},"PeriodicalIF":3.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12875335/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143655606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adipose tissue and androgens: the ins and outs. 脂肪组织和雄激素：来龙去脉。

IF 3.1 4区生物学

Adipocyte Pub Date : 2025-12-01 Epub Date: 2025-06-09 DOI: 10.1080/21623945.2025.2508885

Yiyue Jia, Mathilde Lacombe, Catherine Muller, Delphine Milhas

引用次数: 0

A nomogram based on radiomic features from peri-prostatic adipose tissue for predicting bone metastasis in first-time diagnosed prostate cancer patients. 基于前列腺周围脂肪组织放射学特征预测首次诊断前列腺癌患者骨转移的影像学研究。

IF 3.1 4区生物学

Adipocyte Pub Date : 2025-12-01 Epub Date: 2025-06-19 DOI: 10.1080/21623945.2025.2517583

Bohao Liu, Qian Cai, Xiao Zhao, Huabin Su, Zhengxu Lin, Jialin Wu, Xiaoyang Li, Weian Zhu, Chen Zou, Yun Luo

{"title":"A nomogram based on radiomic features from peri-prostatic adipose tissue for predicting bone metastasis in first-time diagnosed prostate cancer patients.","authors":"Bohao Liu, Qian Cai, Xiao Zhao, Huabin Su, Zhengxu Lin, Jialin Wu, Xiaoyang Li, Weian Zhu, Chen Zou, Yun Luo","doi":"10.1080/21623945.2025.2517583","DOIUrl":"10.1080/21623945.2025.2517583","url":null,"abstract":"Purpose: To evaluate a radiomics-based nomogram using peri-prostatic adipose tissue (PPAT) features for predicting bone metastasis (BM) in newly diagnosed prostate cancer (PCa) patients.Methods: A retrospective study of 151 PCa patients (October 2010-November 2022) was conducted. Radiomic features were extracted from axial T2-weighted MRI of PPAT, and normalized PPAT was calculated as the ratio of PPAT volume to prostate volume. A radiomics score (Radscore) was developed using logistic regression with 16 features selected via LASSO regression. Independent predictors identified through univariate and multivariate logistic regression were used to construct a nomogram. Predictive performance was assessed using ROC curves, and internal validation involved 1000 bootstrapped iterations.Results: The Radscore, based on 16 features, showed significant association with BM and outperformed normalized PPAT in predictive value. Independent predictors of BM included Radscore, alkaline phosphatase (ALP), and clinical N stage (cN). A nomogram integrating these factors demonstrated strong discrimination (C-index: 0.908; 95% CI: 0.851-0.966) and calibration, with consistent results in validation (C-index: 0.903; 95% CI: 0.897-0.916). Decision curve analysis confirmed its clinical utility.Conclusions: Radscore, cN, and ALP were identified as independent BM predictors. The developed nomogram enables accurate risk stratification and personalized BM predictions for newly diagnosed PCa patients.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"14 1","pages":"2517583"},"PeriodicalIF":3.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12184149/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electroacupuncture pretreatment alleviates myocardial ischemia injury via brown adipose tissue and BMP3b/Smad1/5 pathway in mice. 电针预处理通过棕色脂肪组织和BMP3b/Smad1/5通路减轻小鼠心肌缺血损伤。

IF 3.1 4区生物学

Adipocyte Pub Date : 2025-12-01 Epub Date: 2025-11-09 DOI: 10.1080/21623945.2025.2586870

Yuhang Yan, Danying Qian, Xiaohan Lu, Xiaoer Liu, Meiling Yu, Shengfeng Lu

{"title":"Electroacupuncture pretreatment alleviates myocardial ischemia injury via brown adipose tissue and BMP3b/Smad1/5 pathway in mice.","authors":"Yuhang Yan, Danying Qian, Xiaohan Lu, Xiaoer Liu, Meiling Yu, Shengfeng Lu","doi":"10.1080/21623945.2025.2586870","DOIUrl":"10.1080/21623945.2025.2586870","url":null,"abstract":"Acute myocardial infarction (AMI) is a critical condition that induces myocardial ischaemic injury and necrosis, requiring timely intervention to improve outcomes. Emerging evidence suggests that brown adipose tissue (BAT) plays a crucial role in cardiac protection by releasing bone morphogenetic protein 3b (BMP3b), which targets the Smad1/5 pathway in the heart. Here, we investigated whether Electroacupuncture (EA) pretreatment alleviates myocardial injury by activating BAT in AMI mice. The AMI model was established by left coronary artery ligation in male C57BL/6J mice, and EA was applied before AMI model establishment. Comprehensive assessments included morphological and molecular analyses of BAT, cardiac function evaluation, and infarct area measurements. The BMP3b/Smad1/5 signalling pathway was detected in BAT and the heart. Finally, we used mice with scapular BAT removed to verify the pivotal role of BAT in reducing myocardial injury caused by EA. The results showed that EA at ST25 enhanced BAT activation, upregulated BMP3b expression, promoted Smad1/5 phosphorylation, and increased the anti-apoptotic factor Bcl-xL, reducing myocardial damage. However, the protective effect of EA was not observed in the BAT-deficient mice. These findings suggest that EA at ST25 is a promising approach to reduce myocardial injury via the BAT and BMP3b/Smad1/5 pathways.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"14 1","pages":"2586870"},"PeriodicalIF":3.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12604635/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145480471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A compendium of adipocyte morphologies across different breast pathologies. 不同乳腺病理的脂肪细胞形态纲要。

IF 3.1 4区生物学

Adipocyte Pub Date : 2025-12-01 Epub Date: 2025-10-07 DOI: 10.1080/21623945.2025.2568540

Abigail Dodson, Katie Hanna, Kerri Palmer, Hafeez Ibrahim, Katharina Kusserow, Rasha Abu-Eid, Gerald Lip, Nicola Spence, Ehab Husain, Beatrix Elsberger, Justin J Rochford, Valerie Speirs

{"title":"A compendium of adipocyte morphologies across different breast pathologies.","authors":"Abigail Dodson, Katie Hanna, Kerri Palmer, Hafeez Ibrahim, Katharina Kusserow, Rasha Abu-Eid, Gerald Lip, Nicola Spence, Ehab Husain, Beatrix Elsberger, Justin J Rochford, Valerie Speirs","doi":"10.1080/21623945.2025.2568540","DOIUrl":"10.1080/21623945.2025.2568540","url":null,"abstract":"Adipocytes are abundant in the breast tissue microenvironment. In breast cancer, they can change morphologically according to their proximity to tumour cells, with the closest becoming cancer-associated adipocytes (CAAs). It remains unclear whether breast cancer risk factors, including menopausal status, body mass index (BMI), and mammographic density (MD), influence CAAs morphology in breast carcinogenesis. This study aimed to quantify morphological differences in adipocytes across breast cancer pathologies and associated risk factors. Whole slide images of haematoxylin and eosin stained cancer (n = 149) and normal (n = 182) breast tissue samples were analysed. Parameters representative of adipocyte morphology: perimeter, area, concavity, and aspect ratio, were measured using ImageJ. Adipocytes were considered close (≤2 mm) or distant ( > 2 mm) to cancer cells in cancer samples or breast epithelial cells in normal samples. Close adipocytes in cancer samples were designated CAAs. CAAs decreased in size compared to distant adipocytes (p≤0.0001). A similar trend was observed between close and distant adipocytes in normal (p≤0.0001). CAAs size increased post menopause (p ≤ 0.0001). CAAs size positively correlated with BMI (p ≤ 0.0001). In cancer cases, distant adipocyte size increased and concavity decreased with increasing MD (p ≤ 0.01). Smaller CAAs were associated with poorer survival (p≤0.05). Morphological differences were identified in adipocytes dependent on location within the breast, tissue, pathology and risk factors. Understanding what drives these morphological differences could provide mechanistic insight into whether risk factor-induced alterations in adipocytes influence their role in breast carcinogenesis.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"14 1","pages":"2568540"},"PeriodicalIF":3.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12505518/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145237525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0