Adipocyte最新文献_第2页

Low circulating levels of neuregulin 4 as a potential biomarker associated with the severity and prognosis of obesity-related metabolic diseases: a systematic review. 作为与肥胖相关代谢性疾病的严重程度和预后有关的潜在生物标志物的低循环神经胶质蛋白 4：系统综述。

IF 3.5 4区生物学

Adipocyte Pub Date : 2024-12-01 Epub Date: 2024-08-20 DOI: 10.1080/21623945.2024.2390833

Khanyisani Ziqubu, Phiwayinkosi V Dludla, Sinenhlanhla X H Mthembu, Bongani Nkambule, Sithandiwe E Mazibuko-Mbeje

{"title":"Low circulating levels of neuregulin 4 as a potential biomarker associated with the severity and prognosis of obesity-related metabolic diseases: a systematic review.","authors":"Khanyisani Ziqubu, Phiwayinkosi V Dludla, Sinenhlanhla X H Mthembu, Bongani Nkambule, Sithandiwe E Mazibuko-Mbeje","doi":"10.1080/21623945.2024.2390833","DOIUrl":"10.1080/21623945.2024.2390833","url":null,"abstract":"Background: Neuregulin 4 (Nrg4) is a brown adipose tissue-derived adipokine that greatly affects systemic metabolism and improves metabolic derangements. Although abnormal circulating levels of Nrg4 are common in obesity, it remains elusive whether low or elevated levels of this batokine are associated with the onset of metabolic diseases.Aim: To assess Nrg4 levels and its role as a feasible biomarker to predict the severity of obesity, gestational diabetes mellitus (GDM), type 2 diabetes mellitus (T2DM), non-alcoholic fatty liver disease (NAFLD), and cardiovascular diseases (CVD).Methods: A search for relevant studies was performed systematically using prominent search engines, including PubMed, Google Scholar, and Embase, by following PRISMA guidelines.Results: Ample clinical evidence reported low serum/plasma levels of Nrg4 in obesity and these were inversely proportional to the indices of metabolic syndrome, including body mass index, waist circumference, triglycerides, fasting plasma glucose, and homoeostatic model assessment for insulin resistance as well as high-sensitivity C-reactive protein. Low circulating Nrg4 levels may aid in the prediction of morbid obesity, and subsequent GDM, T2DM, NAFLD, and CVD.Conclusion: Current clinical evidence emphasizes that the circulating levels of Nrg4 are decreased in morbid obesity, and it also highlights that Nrg4 May serve as a potential prognostic biomarker for obesity-related metabolic diseases.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"13 1","pages":"2390833"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11340757/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142003372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Follistatin like-1 (Fstl1) regulates adipose tissue development in zebrafish. 卵泡素样蛋白-1 （Fstl1）调节斑马鱼脂肪组织的发育。

IF 3.5 4区生物学

Adipocyte Pub Date : 2024-12-01 Epub Date: 2024-12-07 DOI: 10.1080/21623945.2024.2435862

Lucía Guggeri, Ileana Sosa-Redaelli, Magdalena Cárdenas-Rodríguez, Martina Alonso, Gisell González, Hugo Naya, Victoria Prieto-Echagüe, Paola Lepanto, Jose L Badano

{"title":"Follistatin like-1 (Fstl1) regulates adipose tissue development in zebrafish.","authors":"Lucía Guggeri, Ileana Sosa-Redaelli, Magdalena Cárdenas-Rodríguez, Martina Alonso, Gisell González, Hugo Naya, Victoria Prieto-Echagüe, Paola Lepanto, Jose L Badano","doi":"10.1080/21623945.2024.2435862","DOIUrl":"10.1080/21623945.2024.2435862","url":null,"abstract":"Obesity is a highly prevalent disorder with complex aetiology. Therefore, studying its associated cellular and molecular pathways may be aided by analysing genetic tractable diseases. In this context, the study of ciliopathies such as Bardet-Biedl syndrome has highlighted the relevance of primary cilia in obesity, both in the central nervous system and peripheral tissues. Based on our previous in vitro results supporting the role of a novel Bbs4-cilia-Fstl1 axis in adipocyte differentiation, we evaluated the in vivo relevance of the zebrafish orthologous genes fstl1a and fstl1b in primary cilia and adipose tissue development. Using a combination of knockdowns and a new fstl1a mutant line, we show that fstl1a promotes primary cilia formation in early embryos and participates in adipose tissue formation in larvae. We also show that fstl1b partially compensates for the loss of fstl1a. Moreover, in high fat diet, fstl1a depletion affects the expression of differentiation and mature adipocyte markers. These results agree with our previous in vitro data and provide further support for the role of FSTL1 as a regulator of adipose tissue formation. Dissecting the exact biological role of proteins such as FSTL1 will likely contribute to understand obesity onset and presentation.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"13 1","pages":"2435862"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11633180/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142790889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ojeok-san enhances platinum sensitivity in ovarian cancer by regulating adipocyte paracrine IGF1 secretion. Ojeok-san通过调节脂肪细胞旁分泌IGF1分泌增强卵巢癌的铂敏感性。

IF 3.3 4区生物学

Adipocyte Pub Date : 2024-12-01 Epub Date: 2023-11-22 DOI: 10.1080/21623945.2023.2282566

Jiong Ma, Junyan Li, Xuejun Chen, Yanyan Ma

{"title":"Ojeok-san enhances platinum sensitivity in ovarian cancer by regulating adipocyte paracrine IGF1 secretion.","authors":"Jiong Ma, Junyan Li, Xuejun Chen, Yanyan Ma","doi":"10.1080/21623945.2023.2282566","DOIUrl":"10.1080/21623945.2023.2282566","url":null,"abstract":"Background: Platinum is a commonly used drug for ovarian cancer (OvCa) treatment, but drug resistance limits its clinical application. This study intended to delineate the effects of adipocytes on platinum resistance in OvCa.Methods: OvCa cells were maintained in the adipocyte-conditioned medium. Cell viability and apoptosis were detected by CCK-8 and flow cytometry, separately. Proliferation and apoptosis-related protein expression were assayed by western blot. The IC50 values of cisplatin and carboplatin were determined using CCK-8. IGF1 secretion and expression were assayed via ELISA and western blot, respectively. A xenograft model was established, and pathological changes were detected by H&E staining. Proliferation and apoptosis-associated protein expression was assessed via IHC.Results: Adipocytes promoted the viability and repressed cell apoptosis in OvCa, as well as enhancing platinum resistance, while the addition of IGF-1 R inhibitor reversed the effects of adipocytes on proliferation, apoptosis, and drug resistance of OvCa cells. Treatment with different concentrations of Ojeok-san (OJS) inhibited the adipocyte-induced platinum resistance in OvCa cells by suppressing IGF1. The combined treatment of OJS and cisplatin significantly inhibited tumour growth in vivo with good mouse tolerance.Conclusion: In summary, OJS inhibited OvCa proliferation and platinum resistance by suppressing adipocyte paracrine IGF1 secretion.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"13 1","pages":"2282566"},"PeriodicalIF":3.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10761029/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138294411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

10-Gingerol reduces cytoplasmic lipid droplets and induces lipolysis in 3T3-L1 adipocytes. 10-姜酚可减少细胞质脂滴并诱导 3T3-L1 脂肪细胞的脂肪分解。

IF 3.5 4区生物学

Adipocyte Pub Date : 2024-12-01 Epub Date: 2024-10-10 DOI: 10.1080/21623945.2024.2411453

María Elizabeth Preciado-Ortiz, Erika Martínez-López, Trinidad García-Iglesias, Gildardo Gembe-Olivarez, Nathaly Torres-Castillo, Iris Monserrat Llamas-Covarrubias, Juan José Rivera-Valdés

{"title":"10-Gingerol reduces cytoplasmic lipid droplets and induces lipolysis in 3T3-L1 adipocytes.","authors":"María Elizabeth Preciado-Ortiz, Erika Martínez-López, Trinidad García-Iglesias, Gildardo Gembe-Olivarez, Nathaly Torres-Castillo, Iris Monserrat Llamas-Covarrubias, Juan José Rivera-Valdés","doi":"10.1080/21623945.2024.2411453","DOIUrl":"10.1080/21623945.2024.2411453","url":null,"abstract":"Obesity is a globally prevalent metabolic disorder characterized by an increased number of adipose cells and excessive fat in adipocytes. Herbal medicines, such as ginger, have shown potential in treating obesity by inhibiting adipogenesis and reducing adipocyte hypertrophy. Ginger contains bioactive compounds, particularly gingerols, which have demonstrated anti-adipogenic and/or lipolytic effects. However, research on the effects of 10-gingerol on adipose tissue remains limited. This study aimed to evaluate the effect of 10-gingerol on lipid content, lipolysis markers, and the expression of genes related to lipid metabolism in 3T3-L1 adipocytes. Three groups were analyzed: a negative control (preadipocytes), a positive control (mature adipocytes), and a group treated with 10-gingerol (10-G). Results showed that 10-G reduced lipid accumulation by 42.16% in mature adipocytes compared to the control, without affecting cell viability. Additionally, 10-G increased glycerol release and downregulated lipogenic genes such as Pparγ, Acaca, Fabp4, and Mtor, while upregulating genes related to fatty acid oxidation, including Cebpα, Cpt1a, Lipe, and Prkaa1. In conclusion, 10-gingerol reduces lipid content in mature adipocytes by downregulating lipogenesis, increasing lipolysis, and enhancing fatty acid oxidation.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"13 1","pages":"2411453"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11468046/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142387141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Putative mechanism of a multivitamin treatment against insulin resistance. 多种维生素治疗胰岛素抵抗的假定机制。

IF 3.5 4区生物学

Adipocyte Pub Date : 2024-12-01 Epub Date: 2024-06-27 DOI: 10.1080/21623945.2024.2369777

José Antonio Palma-Jacinto, Edgar López-López, José Luis Medina-Franco, Oreth Montero-Ruíz, Isela Santiago-Roque

{"title":"Putative mechanism of a multivitamin treatment against insulin resistance.","authors":"José Antonio Palma-Jacinto, Edgar López-López, José Luis Medina-Franco, Oreth Montero-Ruíz, Isela Santiago-Roque","doi":"10.1080/21623945.2024.2369777","DOIUrl":"10.1080/21623945.2024.2369777","url":null,"abstract":"Insulin resistance is caused by the abnormal secretion of proinflammatory cytokines in adipose tissue, which is induced by an increase in lipid accumulation in adipocytes, hepatocytes, and myocytes. The inflammatory pathway involves multiple targets such as nuclear factor kappa B, inhibitor of nuclear factor κ-B kinase, and mitogen-activated protein kinase. Vitamins are micronutrients with anti-inflammatory activities that have unclear mechanisms. The present study aimed to describe the putative mechanisms of vitamins involved in the inflammatory pathway of insulin resistance. The strategy to achieve this goal was to integrate data mining and analysis, target prediction, and molecular docking simulation calculations to support our hypotheses. Our results suggest that the multitarget activity of vitamins A, B1, B2, B3, B5, B6, B7, B12, C, D3, and E inhibits nuclear factor kappa B and mitogen-activated protein kinase, in addition to vitamins A and B12 against inhibitor of nuclear factor κ-B kinase. The findings of this study highlight the pharmacological potential of using an anti-inflammatory and multitarget treatment based on vitamins and open new perspectives to evaluate the inhibitory activity of vitamins against nuclear factor kappa B, mitogen-activated protein kinase, and inhibitor of nuclear factor κ-B kinase in an insulin-resistant context.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"13 1","pages":"2369777"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11216102/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141465503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A key role for P2RX5 in brown adipocyte differentiation and energy homeostasis. P2RX5 在棕色脂肪细胞分化和能量平衡中的关键作用

IF 3.5 4区生物学

Adipocyte Pub Date : 2024-12-01 Epub Date: 2024-11-01 DOI: 10.1080/21623945.2024.2421745

Maria Razzoli, Seth McGonigle, Bhavani Shankar Sahu, Pedro Rodriguez, Daniel Svedberg, Loredana Rao, Chiara Ruocco, Enzo Nisoli, Bianca Vezzani, Andrea Frontini, Alessandro Bartolomucci

{"title":"A key role for P2RX5 in brown adipocyte differentiation and energy homeostasis.","authors":"Maria Razzoli, Seth McGonigle, Bhavani Shankar Sahu, Pedro Rodriguez, Daniel Svedberg, Loredana Rao, Chiara Ruocco, Enzo Nisoli, Bianca Vezzani, Andrea Frontini, Alessandro Bartolomucci","doi":"10.1080/21623945.2024.2421745","DOIUrl":"10.1080/21623945.2024.2421745","url":null,"abstract":"Brown adipocytes are defined based on a distinct morphology and genetic signature that includes, amongst others, the expression of the Purinergic 2 Receptor X5 (P2RX5). However, the role of P2RX5 in brown adipocyte and brown adipose tissue function is poorly characterized. In the present study, we conducted a metabolic characterization of P2RX5 knockout male mice; next, we characterized this purinergic pathway in a cell-autonomous context in brown adipocytes. We then tested the role of the P2RX5 receptor agonism in metabolic responses in vivo in conditions of minimal adaptive thermogenesis requirements. Our data show that loss of P2RX5 causes reduced brown adipocyte differentiation in vitro, and browning in vivo. Lastly, we unravel a previously unappreciated role for P2RX5 agonism to exert an anti-obesity effect in the presence of enhanced brown adipose tissue recruitment in male mice housed at thermoneutrality. Altogether, our data support a role for P2RX5 in mediating brown adipocyte differentiation and function that could be further targeted for benefits in the context of adipose tissue pathology and metabolic diseases.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"13 1","pages":"2421745"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11540092/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142556785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and adipogenic differentiation of murine mesenchymal stem cells harvested from macrophage-depleted bone marrow and adipose tissue. 从去除了巨噬细胞的骨髓和脂肪组织中获得的小鼠间充质干细胞的分离和成脂分化。

IF 3.5 4区生物学

Adipocyte Pub Date : 2024-12-01 Epub Date: 2024-06-11 DOI: 10.1080/21623945.2024.2350751

Iram Fatima S Siddiqui, Muthu L Muthu, Dieter P Reinhardt

{"title":"Isolation and adipogenic differentiation of murine mesenchymal stem cells harvested from macrophage-depleted bone marrow and adipose tissue.","authors":"Iram Fatima S Siddiqui, Muthu L Muthu, Dieter P Reinhardt","doi":"10.1080/21623945.2024.2350751","DOIUrl":"10.1080/21623945.2024.2350751","url":null,"abstract":"Introduction and purpose: Mouse mesenchymal stem cells (MSCs) provide a resourceful tool to study physiological and pathological aspects of adipogenesis. Bone marrow-derived MSCs (BM-MSCs) and adipose tissue-derived MSCs (ASCs) are widely used for these studies. Since there is a wide spectrum of methods available, the purpose is to provide a focused hands-on procedural guide for isolation and characterization of murine BM-MSCs and ASCs and to effectively differentiate them into adipocytes.Methods and results: Optimized harvesting procedures for murine BM-MSCs and ASCs are described and graphically documented. Since macrophages reside in bone-marrow and fat tissues and regulate the biological behaviour of BM-MSCs and ASCs, we included a procedure to deplete macrophages from the MSC preparations. The identity and stemness of BM-MSCs and ASCs were confirmed by flow cytometry using established markers. Since the composition and concentrations of adipogenic differentiation cocktails differ widely, we present a standardized four-component adipogenic cocktail, consisting of insulin, dexamethasone, 3-isobutyl-1-methylxanthine, and indomethacin to efficiently differentiate freshly isolated or frozen/thawed BM-MSCs and ASCs into adipocytes. We further included visualization and quantification protocols of the differentiated adipocytes.Conclusion: This laboratory protocol was designed as a step-by-step procedure for harvesting murine BM-MSCs and ASCs and differentiating them into adipocytes.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"13 1","pages":"2350751"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11174124/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141299746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantification of cell cycle re-entry during dedifferentiation of primary adipocytes in vitro. 体外原代脂肪细胞再分化过程中细胞周期再进入的量化。

IF 3.5 4区生物学

Adipocyte Pub Date : 2024-12-01 Epub Date: 2024-07-11 DOI: 10.1080/21623945.2024.2376571

Ewa Bielczyk-Maczynska

引用次数: 0

Comparison of stromal vascular fraction cell composition between Coleman fat and extracellular matrix/stromal vascular fraction gel. 比较科尔曼脂肪和细胞外基质/基质血管部分凝胶的基质血管部分细胞组成。

IF 3.5 4区生物学

Adipocyte Pub Date : 2024-12-01 Epub Date: 2024-06-03 DOI: 10.1080/21623945.2024.2360037

Xiaoyun Li, Guohong Zhang, Mengmeng Wang, Changhao Lu, Guangping Zhang, Zhehui Chen, Yingchang Ji

{"title":"Comparison of stromal vascular fraction cell composition between Coleman fat and extracellular matrix/stromal vascular fraction gel.","authors":"Xiaoyun Li, Guohong Zhang, Mengmeng Wang, Changhao Lu, Guangping Zhang, Zhehui Chen, Yingchang Ji","doi":"10.1080/21623945.2024.2360037","DOIUrl":"10.1080/21623945.2024.2360037","url":null,"abstract":"As a mechanically condensed product of Coleman fat, extracellular matrix/stromal vascular fraction gel (ECM/SVF-gel) eliminates adipocytes, concentrates SVF cells, and improves fat graft retention. This study aims to compare SVF cell composition between Coleman fat and ECM/SVF-gel. Matched Coleman fat and ECM/SVF-gel of 28 healthy women were subjected to RNA-seq, followed by functional enrichment and cell-type-specific enrichment analyses, and deconvolution of SVF cell subsets, reconstructing SVF cell composition in the transcriptome level. ECM/SVF-gels had 9 upregulated and 73 downregulated differentially expressed genes (DEGs). Downregulated DEGs were mainly associated with inflammatory and immune responses, and enriched in fat macrophages. M2 macrophages, resting CD4+ memory T cells, M1 macrophages, resting mast cells, and M0 macrophages ranked in the top five most prevalent immune cells in the two groups. The proportions of the principal non-immune cells (e.g., adipose-derived stem cells, pericytes, preadipocytes, microvascular endothelial cells) had no statistical differences between the two groups. Our findings reveal ECM/SVF-gels share the same dominant immune cells beneficial to fat graft survival with Coleman fat, but exhibiting obvious losses of immune cells (especially macrophages), while non-immune cells necessary for adipose regeneration might have no significant loss in ECM/SVF-gels and their biological effects could be markedly enhanced by the ECM/SVF-gel's condensed nature.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"13 1","pages":"2360037"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11152101/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141199244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An improved in vitro 3T3-L1 adipocyte model of inflammation and insulin resistance. 改进的体外 3T3-L1 脂肪细胞炎症和胰岛素抵抗模型。

IF 3.5 4区生物学

Adipocyte Pub Date : 2024-12-01 Epub Date: 2024-10-17 DOI: 10.1080/21623945.2024.2414919

Ifeoluwa A Odeniyi, Bulbul Ahmed, Benjamin Anbiah, Grace Hester, Peter T Abraham, Elizabeth A Lipke, Michael W Greene

{"title":"An improved in vitro 3T3-L1 adipocyte model of inflammation and insulin resistance.","authors":"Ifeoluwa A Odeniyi, Bulbul Ahmed, Benjamin Anbiah, Grace Hester, Peter T Abraham, Elizabeth A Lipke, Michael W Greene","doi":"10.1080/21623945.2024.2414919","DOIUrl":"https://doi.org/10.1080/21623945.2024.2414919","url":null,"abstract":"Tumor necrosis factor alpha (TNF-α)/hypoxia-treated 3T3-L1 adipocytes have been used to model inflamed and insulin-resistant adipose tissue: this study examines gaps in the model. We tested whether modulating TNF-α/hypoxia treatment time could reduce cell death while still inducing inflammation and insulin resistance. Adipocytes were treated with TNF-α (12 h or 24 h) and incubated in a hypoxic chamber for 24 h. To examine maintenance of the phenotype over time, glucose and FBS were added at 24 h post initiation of treatment, and the cells were maintained for an additional 48 h. Untreated adipocytes were used as a control. Viability, insulin resistance, and inflammation were assessed using Live/Dead staining, RT-qPCR, ELISA, and glucose uptake assays. Treatment for 12 h with TNF-α in the presence of hypoxia resulted in an increase in the percentage of live cells compared to 24 h treated cells. Importantly, insulin resistance and inflammation were still induced in the 12 h treated adipocytes: the expression of the insulin sensitive and inflammatory genes was decreased and increased, respectively. In 72 h treated adipocytes, no significant differences were found in the viability, glucose uptake or insulin-sensitive and inflammatory gene expression. This study provides a modified approach to in vitro odeling adipocyte inflammation and insulin resistance. .","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"13 1","pages":"2414919"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11487959/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0