Adipocyte最新文献_第3页

Adipogenic characterization of immortalized CD55⁺ progenitor cells from human white adipose tissue. 人白色脂肪组织中永生化CD55+祖细胞的成脂特性。

IF 3.5 4区生物学

Adipocyte Pub Date : 2025-12-01 Epub Date: 2025-06-05 DOI: 10.1080/21623945.2023.2283213

Morgane Couchet, Hui Gao, Felix Klingelhuber, Jutta Jalkanen, Thais De Castro Barbosa, Muhmmad Omar-Hmeadi, Lucas Massier, Natalie Krahmer, Niklas Mejhert, Mikael Rydén

{"title":"Adipogenic characterization of immortalized CD55+ progenitor cells from human white adipose tissue.","authors":"Morgane Couchet, Hui Gao, Felix Klingelhuber, Jutta Jalkanen, Thais De Castro Barbosa, Muhmmad Omar-Hmeadi, Lucas Massier, Natalie Krahmer, Niklas Mejhert, Mikael Rydén","doi":"10.1080/21623945.2023.2283213","DOIUrl":"10.1080/21623945.2023.2283213","url":null,"abstract":"Background: Mature adipocytes are difficult to study ex vivo, prompting the use of human adipose progenitor cells (hAPCs). However, hAPCs undergo replicative senescence, limiting their utility in long-term studies.Methods: We inserted human telomerase reverse transcriptase (TERT) into the AAVS1 locus of CD55+ hAPCs derived from abdominal subcutaneous adipose tissue, and characterized the cells before and after adipogenic differentiation.Results: TERT-hAPCs retained proliferative and adipogenic capacities for over 80 passages, comparable to early-passage wild type hAPCs. Transcriptomic and proteomic analyses confirmed strong adipocyte gene expression. Functionally, TERT-hAPCs responded to insulin and lipolytic stimuli (isoprenaline, dibutyryl cAMP, TNF-α). They adapted well to both 2D and 3D cultures, with improved adipogenesis under spheroid conditions.Conclusion: Immortalization of CD55+ hAPCs yields cells with stable proliferative and adipogenic capacity across passages. Being cryopreservable and suitable for both 2D and 3D cultures, TERT-hAPCs offer a reliable, reusable model system for adipocyte studies using cells with a consistent genetic background.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":" ","pages":"2283213"},"PeriodicalIF":3.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12147497/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138045962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electroacupuncture stimulation induced M1/M2 polarization in white adipose tissues by activating the Y1 receptor in obese mice to reduce chronic inflammation. 电针刺激通过激活肥胖小鼠Y1受体，诱导白色脂肪组织M1/M2极化，减轻慢性炎症。

IF 3.5 4区生物学

Adipocyte Pub Date : 2025-12-01 Epub Date: 2025-06-30 DOI: 10.1080/21623945.2025.2524638

Mengjiang Lu, Ziwei Yu, Xingyu Yang, Ze Yang, Tiancheng Xu, Zhi Yu, Xinyue Jing, Li An, Jianbin Zhang, Bin Xu

{"title":"Electroacupuncture stimulation induced M1/M2 polarization in white adipose tissues by activating the Y1 receptor in obese mice to reduce chronic inflammation.","authors":"Mengjiang Lu, Ziwei Yu, Xingyu Yang, Ze Yang, Tiancheng Xu, Zhi Yu, Xinyue Jing, Li An, Jianbin Zhang, Bin Xu","doi":"10.1080/21623945.2025.2524638","DOIUrl":"10.1080/21623945.2025.2524638","url":null,"abstract":"Chronic inflammation in obesity can induce complications such as diabetes and cardiovascular disease. Visceral adipose tissue is the main source of inflammation, but it is difficult to regulate effectively. Here, we investigated whether ES suppressed inflammation in eWAT and reduced chronic inflammation in obese individuals. We established a high-fat diet (HFD) model with C57BL/6J mice to measure chronic inflammation in obesity. In addition, the sympathetic nerve activity (SNA) was measured with the electrophysiological technique, the immunostaining and flow cytometry were used to detect the Y1 receptors in macrophage. Finally, the key role of the M1/M2 polarization in white adipose tissues by activating the Y1 receptor was verified by Y1 receptor antagonist BIBP3226. ES reduced the contents of IL-1β, TNF-α, IL-6 and TGF-β in the plasma and the mRNA expression of il-1 and tnfα in eWAT. Also, ES suppressed SNA in eWAT which regulated NPY1R receptor. In addition, ES induced M1/M2 polarization in eWAT via the Y1 receptor. The injection of a Y1 receptor (NPY1R) antagonist BIBP3226 restrained M1/M2 polarization. Further studies revealed that ES regulated sympathetic axons in eWAT to activate the Y1 receptor. This research demonstrates ES reduce chronic inflammation e mechanism is associated with the sympathetic Y1 receptor pathway, which promotes M1/M2 polarization.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"14 1","pages":"2524638"},"PeriodicalIF":3.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12218457/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144525976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D imaging and quantitative analysis of adipocytes in situ and ex situ. 原位和非原位脂肪细胞的三维成像和定量分析。

IF 3.1 4区生物学

Adipocyte Pub Date : 2025-12-01 Epub Date: 2025-09-21 DOI: 10.1080/21623945.2025.2558573

Isabelle Hue, Adèle Branthonne, Manon Thomas, Violette Thermes, Jérôme Bugeon

{"title":"3D imaging and quantitative analysis of adipocytes in situ and ex situ.","authors":"Isabelle Hue, Adèle Branthonne, Manon Thomas, Violette Thermes, Jérôme Bugeon","doi":"10.1080/21623945.2025.2558573","DOIUrl":"10.1080/21623945.2025.2558573","url":null,"abstract":"Different adipose tissues (AT) have been described, including subcutaneous and visceral tissues (SCAT and VAT). They display different morphological structures, physiological and metabolic functions. Imaging adipocytes in the whole AT was not feasible because of the large adipocyte sizes and the lipid-full content of the droplets that increased the refractive index. Tissue clearing is then required mainly through a delipidation step, which induces also a tissue shrinkage. Our aim was to image in 3D freshly extracted adipocytes and compare them to those within their tissues. Trout ATs were stained with 5DTAF (extracellular matrix) and Nile Red (lipids). After clearing with Histodenz, 3D images were obtained using a confocal microscope, and adipocytes were segmented and measured. In situ, major differences in adipocyte size and shape were observed between the VAT and SCAT. Ex situ, only the size mattered because all cells were round outside their tissues. This method can be applied to other species, such as mice. In situ, adipocyte sphericity was even higher in the SCAT from a Swiss and a C57Bl6. This approach demonstrates that 3D adipocyte imaging with lipid labeling enables accurate morphological characterization, provides insights into depot-specific structural features, and supports optimization of cell isolation protocols.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"14 1","pages":"2558573"},"PeriodicalIF":3.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12456214/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145111667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrastructural characterization of white adipocytes in a mouse model with enhanced sequestration of fatty acids in adipose tissue. 脂肪组织中脂肪酸吸收增强的小鼠模型中白色脂肪细胞的超微结构表征。

IF 3.5 4区生物学

Adipocyte Pub Date : 2025-12-01 Epub Date: 2025-07-14 DOI: 10.1080/21623945.2025.2531829

Yi Zhang, Keigo Tomoo, Yen-Hsi Lai, Gregory C Henderson

{"title":"Ultrastructural characterization of white adipocytes in a mouse model with enhanced sequestration of fatty acids in adipose tissue.","authors":"Yi Zhang, Keigo Tomoo, Yen-Hsi Lai, Gregory C Henderson","doi":"10.1080/21623945.2025.2531829","DOIUrl":"10.1080/21623945.2025.2531829","url":null,"abstract":"Sequestration of free fatty acids (FFA) inside white adipose tissue (WAT) may reduce plasma FFA levels and prevent lipotoxicity in other organs. However, it is poorly understood how WAT responds to this metabolic stress. As albumin promotes FFA release from WAT, and thus albumin deficiency should promote FFA sequestration, we studied albumin knockout (Alb-/-) mice and their wildtype (WT) littermates (eight-week-old males). Transmission electron microscopy and molecular analyses were used for characterization. There was no significant difference between genotypes for WAT mass, adipocyte size or triacylglycerol (TAG) content. No signs of cell death were observed in Alb-/- adipocytes, suggesting a tolerance to the metabolic challenge. Alb-/- adipocytes exhibited a lower density of caveolae with smaller invagination depths, indicating a potential adaptation to reduce FFA transport. A significantly higher abundance of micro-lipid droplets was observed in Alb-/- mice, which may result from a rapid substrate cycle with high lipolysis and re-esterification. In support of the ultrastructural phenotype, lipidomic analysis also demonstrated a significant difference between Alb-/- and WT for TAG composition. Our results showed that when no albumin is present to facilitate FFA mobilization, WAT can chronically adapt to protect the adipocytes in both morphological and molecular manners.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"14 1","pages":"2531829"},"PeriodicalIF":3.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12269660/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144625265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Activation of CXCR7 exerts an inhibitory effect on adipogenesis through regulation of β-arrestin2/Wnt and AKT signalling. CXCR7的激活通过调节β-arrestin2/Wnt和AKT信号通路，对脂肪形成起到抑制作用。

IF 3.5 4区生物学

Adipocyte Pub Date : 2025-12-01 Epub Date: 2025-04-29 DOI: 10.1080/21623945.2025.2490258

Shiyue Sun, Muhammad Arif Aslam, Eun Bi Ma, Gahui Lee, Hafiz Muhammad Ahmad Javaid, Somy Yoon, Joo Young Huh

{"title":"Activation of CXCR7 exerts an inhibitory effect on adipogenesis through regulation of β-arrestin2/Wnt and AKT signalling.","authors":"Shiyue Sun, Muhammad Arif Aslam, Eun Bi Ma, Gahui Lee, Hafiz Muhammad Ahmad Javaid, Somy Yoon, Joo Young Huh","doi":"10.1080/21623945.2025.2490258","DOIUrl":"https://doi.org/10.1080/21623945.2025.2490258","url":null,"abstract":"CXCR7, an alternative receptor for the inflammatory chemokine SDF-1, is involved in cell proliferation and migration. Recent studies have reported that CXCR7 also plays a role in adipose tissue. However, evidence regarding the role of CXCR7 and its ligands in adipocyte differentiation is limited. In this study, we aimed to elucidate changes in CXCR7 expression during adipocyte differentiation and the role of the SDF-1/CXCR7/CXCR4 axis in adipogenesis using recombinant SDF-1, the CXCR7 ligand CCX771, and small interfering RNAs. The results indicated that the levels of SDF-1 and its receptors, CXCR7 and CXCR4, decreased during the early stages of adipogenesis. Treatment with recombinant SDF-1 and CCX771 inhibited adipogenesis and lipid accumulation by inducing β-arrestin2, Wnt expression, and AKT phosphorylation and downregulating C/EBPα, PPARγ, and FABP4 expression. In contrast, knockdown of SDF-1 and CXCR7 in preadipocytes downregulated the β-arrestin2/Wnt and AKT pathway, leading to the induction of adipogenesis. Meanwhile, knockdown of CXCR4 had no significant effect. In mice, basal gene expression levels of SDF-1 and CXCR7 were higher in the stromal vascular fraction compared to mature adipocytes and were significantly upregulated by a high-fat diet. Our results provide new insights into the local role of the SDF-1-CXCR7 axis in adipocytes and offer additional benefits for the prevention of obesity-related metabolic disorders.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"14 1","pages":"2490258"},"PeriodicalIF":3.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12045560/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retinoid X receptor γ interacts with peroxisome proliferator-activated receptor-γ to promote browning during adipose tissue differentiation. 类视黄醇X受体γ与过氧化物酶体增殖物激活受体γ相互作用，促进脂肪组织分化过程中的褐变。

IF 3.1 4区生物学

Adipocyte Pub Date : 2025-12-01 Epub Date: 2025-08-25 DOI: 10.1080/21623945.2025.2548780

Defei Chen, Saed Woraikat, Xiong Guo, Fuyu Yang, Chenglin Tang, Fan He, Kun Qian

{"title":"Retinoid X receptor γ interacts with peroxisome proliferator-activated receptor-γ to promote browning during adipose tissue differentiation.","authors":"Defei Chen, Saed Woraikat, Xiong Guo, Fuyu Yang, Chenglin Tang, Fan He, Kun Qian","doi":"10.1080/21623945.2025.2548780","DOIUrl":"https://doi.org/10.1080/21623945.2025.2548780","url":null,"abstract":"Obesity and type 2 diabetes mellitus are global public health challenges. Activating thermogenic adipose tissues, such as brown adipose tissue and beige adipose tissue, could be a promising strategy to combat obesity and consequently obesity-related diabetes. Both peroxisome proliferator-activated receptor-γ (PPARγ) and retinoid X receptor γ (RXRγ) play significant roles in the regulation of adipogenic differentiation. However, the underlying mechanisms and interactions between these receptors during adipogenic differentiation remain unclear. In this study, we conducted a comprehensive analysis of a transcriptome sequencing dataset sourced from the GEO database, encompassing samples of white and brown adipose tissues from 15 healthy individuals. Our findings reveal that RXRγ expression is significantly elevated in brown adipose tissue relative to white adipose tissue (p = 0.041). Furthermore, co-immunoprecipitation assays validated that RXRγ can be co-precipitated with PPARγ. Subsequent luciferase assays demonstrated that the interaction between RXRγ and PPARγ significantly enhances the transcriptional activity of uncoupling protein 1 (UCP1) compared to the overexpression of PPARγ alone (3.4-fold vs. 1.5-fold, p < 0.001). Notably, in human preadipocytes, the co-overexpression of RXRγ with PPARγ resulted in a significant increase in UCP1 transcriptional activity compared to the overexpression of PPARγ alone (3.4-fold vs. 2.0-fold, p < 0.05). In summary, our findings suggest that RXRγ serves as a novel cofactor for PPARγ, promoting the browning of adipose tissue through the upregulation of UCP1 transcription.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"14 1","pages":"2548780"},"PeriodicalIF":3.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12382473/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144938068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FLOT chemotherapy treatment affects adipocyte's lipid metabolism: an in vitro study. FLOT化疗对脂肪细胞脂质代谢影响的体外研究。

IF 3.1 4区生物学

Adipocyte Pub Date : 2025-12-01 Epub Date: 2025-06-21 DOI: 10.1080/21623945.2025.2518285

Lisa Guerrier, Ruddy Richard, Jean Brac de la Perrière, Ophélie Bacoeur-Ouzillou, Julianne Touron, Johan Gagnière, Alexandre Pinel, Corinne Malpuech-Brugère

{"title":"FLOT chemotherapy treatment affects adipocyte's lipid metabolism: an in vitro study.","authors":"Lisa Guerrier, Ruddy Richard, Jean Brac de la Perrière, Ophélie Bacoeur-Ouzillou, Julianne Touron, Johan Gagnière, Alexandre Pinel, Corinne Malpuech-Brugère","doi":"10.1080/21623945.2025.2518285","DOIUrl":"10.1080/21623945.2025.2518285","url":null,"abstract":"Cachexia is a complex syndrome that is often associated with cancer. Chemotherapy, one of the main cancer treatments, worsens weight loss in cancer-induced cachexia. In this context, it is thought that fat loss precedes muscle loss, and that alterations in adipose tissue are associated with tumours. However, the effect of cancer treatment on adipose tissue is not well understood. This study aimed to evaluate the impact of chemotherapy alone on mature 3T3-L1 adipocytes to identify the mechanisms contributing to adipose tissue alteration. The murine cell line 3T3-L1, a model of mature adipocytes, was used in this study. After differentiation, cells were treated for 48 h with a chemotherapy cocktail called FLOT composed of 5-fluorouracil, leucovorin, oxaliplatin and docetaxel at two concentrations (FLOT 1X and 0.1X). The control group was treated with the vehicle of the chemotherapy cocktail. Viability, mitochondrial function and dynamics, lipid metabolism, and cellular stress were also evaluated. FLOT 1X chemotherapy significantly reduced viability of mature 3T3-L1 cells and inhibited lipid accumulation. Interestingly, while FLOT 1X treatment downregulated lipogenesis markers, FLOT 0.1X treatment upregulated some of them. Although, the treatment showed no effect on mitochondrial respiration or density, it significantly increased expression of oxidative stress and inflammation markers in adipocytes.This in vitro study provides the first evidence of FLOT chemotherapy's direct effects on healthy mature adipocytes. The results demonstrate significant treatment-induced reductions in cell viability along with dysregulation of both lipogenic and lipolytic pathways. These findings elucidate previously unrecognized mechanisms underlying adipose tissue dysfunction in cancer cachexia.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"14 1","pages":"2518285"},"PeriodicalIF":3.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12445512/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144339800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of alpelisib treatment on murine Pten-deficient lipomas. alpelisib对小鼠pten缺陷型脂肪瘤的治疗作用。

IF 3.5 4区生物学

Adipocyte Pub Date : 2025-12-01 Epub Date: 2025-02-17 DOI: 10.1080/21623945.2025.2468275

Lea M Merz, Karsten Winter, Sandy Richter, Sonja Kallendrusch, Andreas Horn, Sonja Grunewald, Nora Klöting, Kerstin Krause, Wieland Kiess, Diana Le Duc, Antje Garten

{"title":"Effects of alpelisib treatment on murine Pten-deficient lipomas.","authors":"Lea M Merz, Karsten Winter, Sandy Richter, Sonja Kallendrusch, Andreas Horn, Sonja Grunewald, Nora Klöting, Kerstin Krause, Wieland Kiess, Diana Le Duc, Antje Garten","doi":"10.1080/21623945.2025.2468275","DOIUrl":"10.1080/21623945.2025.2468275","url":null,"abstract":" Phosphatase and tensin homolog (PTEN) hamartoma tumour syndrome (PHTS) is a rare disorder caused by germline mutations in the tumour suppressor gene PTEN, a key negative regulator of phosphatidylinositol 3-kinase (PI3K)/AKT signalling. Children with PHTS often develop lipomas, for which only surgical resection is available as treatment. We investigated the effects of the selective PI3K-inhibitor alpelisib on Pten-deficient lipomas. After incubation with alpelisib or the non-selective PI3K inhibitor wortmannin, we analysed histology, gene expression, and Pi3k pathway in lipoma and control epididymal adipose tissue (epiWAT). Alpelisib increased adipocyte area in lipomas compared to epiWAT. Baseline gene expression showed higher levels of markers for proliferation (Pcna), fibrosis (Tgfb1), and adipogenesis (Pparg) in lipomas, while hormone-sensitive lipase expression was lower than in epiWAT. Following alpelisib incubation, target genes of Pi3k signalling and extracellular matrix factors were reduced. We confirmed Pi3k inhibition through detecting decreased Akt levels compared to control treatment. Human lipoma samples treated with alpelisib showed variable lipolysis responses, suggesting variability in therapeutic outcomes. We established an ex vivo model to study alpelisib effects on Pten-deficient lipomas. These results underscore the therapeutic potential of targeted PI3K inhibition in the treatment of PHTS-associated lipomas, particularly in cases that are inoperable.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"14 1","pages":"2468275"},"PeriodicalIF":3.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11844927/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Perivascular adipocyte size is related to the lipid profile and inflammatory changes in a healthy population. 在健康人群中，血管周围脂肪细胞大小与脂质分布和炎症变化有关。

IF 3.5 4区生物学

Adipocyte Pub Date : 2025-12-01 Epub Date: 2025-05-23 DOI: 10.1080/21623945.2025.2499500

Hana Bartuskova, Ivana Kralova Lesna, Sona Kauerova, Vera Lanska, Jiri Fronek, Libor Janousek, Barbora Muffova, Karel Paukner, Rudolf Poledne

{"title":"Perivascular adipocyte size is related to the lipid profile and inflammatory changes in a healthy population.","authors":"Hana Bartuskova, Ivana Kralova Lesna, Sona Kauerova, Vera Lanska, Jiri Fronek, Libor Janousek, Barbora Muffova, Karel Paukner, Rudolf Poledne","doi":"10.1080/21623945.2025.2499500","DOIUrl":"10.1080/21623945.2025.2499500","url":null,"abstract":"Inflammatory changes in perivascular adipose tissue are associated with atherosclerotic lesions in the adjacent artery and can also be used as a marker in patient workup. While adipocyte size is known to be closely related to adipose tissue dysfunction and inflammation, it has not been widely studied in perivascular adipose tissue obtained from healthy human subjects without clinical atherosclerosis. In this cross-sectional study, we addressed this issue by measuring adipocyte size and defining its relationship to cardiovascular risk factors in a healthy cohort of living kidney donors. The presence of cardiovascular risk factors was established by a standardized questionnaire, clinical measurements and body composition analyses. Adipocyte size was measured in the perivascular depot. The proportions of various macrophage subtypes were determined by flow cytometry. To confirm the results, the proportion of CD68 + macrophages was additionally assessed by immunohistochemistry. A correlation and principal component analyses were performed to explore associations. Adipocyte size in perivascular adipose tissue correlated with markers of lipid metabolism, inflammation, and glucose metabolism. Further, the positive correlation with the pro-inflammatory subpopulation of macrophages suggests a strong local effect of perivascular adipose tissue. Perivascular adipocyte size was associated with cardiovascular risk factors and markers of inflammation in a healthy cohort of living kidney donors. This further supports the local role of adipose tissue dysfunction and inflammation in early atherosclerosis development and detection.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"14 1","pages":"2499500"},"PeriodicalIF":3.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12118406/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Involvement of a battery of investigated genes in lipid droplet pathophysiology and associated comorbidities. 脂滴病理生理学和相关并发症中的一系列研究基因的参与。

IF 3.5 4区生物学

Adipocyte Pub Date : 2024-12-01 Epub Date: 2024-09-27 DOI: 10.1080/21623945.2024.2403380

Sami N Al Harake, Yasamin Abedin, Fatema Hatoum, Nour Zahraa Nassar, Ali Ali, Aline Nassar, Amjad Kanaan, Samer Bazzi, Sami Azar, Frederic Harb, Hilda E Ghadieh

引用次数: 0