Adipocyte最新文献_第3页

Inhibiting TRIM8 alleviates adipocyte inflammation and insulin resistance by regulating the DUSP14/MAPKs pathway. 抑制 TRIM8 可通过调节 DUSP14/MAPKs 通路缓解脂肪细胞炎症和胰岛素抵抗。

IF 3.5 4区生物学

Adipocyte Pub Date : 2024-12-01 Epub Date: 2024-07-22 DOI: 10.1080/21623945.2024.2381262

Mingxue Zhu, Junliang Pu, Ting Zhang, Huarui Shao, Rui Su, Chengyong Tang

{"title":"Inhibiting TRIM8 alleviates adipocyte inflammation and insulin resistance by regulating the DUSP14/MAPKs pathway.","authors":"Mingxue Zhu, Junliang Pu, Ting Zhang, Huarui Shao, Rui Su, Chengyong Tang","doi":"10.1080/21623945.2024.2381262","DOIUrl":"10.1080/21623945.2024.2381262","url":null,"abstract":"Obesity is a low-grade chronic inflammation induced by the pathological expansion of adipocytes which allows the development of obesity-associated metabolic diseases like type 2 diabetes mellitus (T2D) and non-alcoholic fatty liver disease (NAFLD). However, mechanisms regulating adipocyte inflammation remain poorly understood. Here, we observed that TRIM8 was upregulated in adipocyte inflammation and insulin resistance while DUSP14 was downregulated. TRIM8 deficiency and DUSP14 over-expression decreased the level of inflammatory cytokines, increased glucose uptake content, and improved insulin signalling transduction compared to LPS treatment alone. Conversely, silencing DUSP14 increased the expression of inflammatory cytokines. It decreased the glucose uptake content and the phosphorylation level of proteins involved in insulin signalling, further impairing insulin signalling and aggravating insulin resistance. Furthermore, The decreased level of inflammatory cytokines, increased glucose uptake, and improved insulin signalling transduction caused by TRIM8 deficiency were reversed by down-regulated DUSP14. Collectively, our findings revealed that TRIM8 can regulate adipocyte inflammation and insulin resistance by regulating the MAPKs pathway which is dependent on DUSP14.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"13 1","pages":"2381262"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11268219/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141747144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding temporal thermogenesis: coregulator selectivity and transcriptional control in brown and beige adipocytes. 解码时间性产热：棕色和米色脂肪细胞中核心调节器的选择性和转录控制。

IF 3.5 4区生物学

Adipocyte Pub Date : 2024-12-01 Epub Date: 2024-08-18 DOI: 10.1080/21623945.2024.2391511

Yong Geun Jeon, Sun Won Kim, Jae Bum Kim

{"title":"Decoding temporal thermogenesis: coregulator selectivity and transcriptional control in brown and beige adipocytes.","authors":"Yong Geun Jeon, Sun Won Kim, Jae Bum Kim","doi":"10.1080/21623945.2024.2391511","DOIUrl":"10.1080/21623945.2024.2391511","url":null,"abstract":"In mammals, brown adipose tissue (BAT) and beige adipocytes in white adipose tissue (WAT) play pivotal roles in maintaining body temperature and energy metabolism. In mice, BAT quickly stimulates thermogenesis by activating brown adipocytes upon cold exposure. In the presence of chronic cold stimuli, beige adipocytes are recruited in inguinal WAT to support heat generation. Accumulated evidence has shown that thermogenic execution of brown and beige adipocytes is regulated in a fat depot-specific manner. Recently, we have demonstrated that ubiquitin ligase ring finger protein 20 (RNF20) regulates brown and beige adipocyte thermogenesis through fat-depot-specific modulation. In BAT, RNF20 regulates transcription factor GA-binding protein alpha (GABPα), whereas in inguinal WAT, RNF20 potentiates transcriptional activity of peroxisome proliferator-activated receptor-gamma (PPARγ) through the degradation of nuclear corepressor 1 (NCoR1). This study proposes the molecular mechanisms by which co-regulator(s) selectively and temporally control transcription factors to coordinate adipose thermogenesis in a fat-depot-specific manner. In this Commentary, we provide molecular features of brown and beige adipocyte thermogenesis and discuss the underlying mechanisms of distinct thermogenic processes in two fat depots.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"13 1","pages":"2391511"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11340756/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141999266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The change of epicardial adipose tissue characteristics and vulnerability for atrial fibrillation upon drastic weight loss. 体重急剧下降时心外膜脂肪组织特征和心房颤动易感性的变化

IF 3.5 4区生物学

Adipocyte Pub Date : 2024-12-01 Epub Date: 2024-09-09 DOI: 10.1080/21623945.2024.2395565

Eva R Meulendijks, Carolina Janssen-Telders, Elise L Hulsman, Nick Lobe, Pietro Zappala, Marc M Terpstra, Robin Wesselink, Tim A C de Vries, Rushd F Al-Shama, Ruben N van Veen, Steve M M de Castro, Claire E E de Vries, Leontien M G Nijland, R Nils Planken, Sebastien P J Krul, Joris R de Groot

{"title":"The change of epicardial adipose tissue characteristics and vulnerability for atrial fibrillation upon drastic weight loss.","authors":"Eva R Meulendijks, Carolina Janssen-Telders, Elise L Hulsman, Nick Lobe, Pietro Zappala, Marc M Terpstra, Robin Wesselink, Tim A C de Vries, Rushd F Al-Shama, Ruben N van Veen, Steve M M de Castro, Claire E E de Vries, Leontien M G Nijland, R Nils Planken, Sebastien P J Krul, Joris R de Groot","doi":"10.1080/21623945.2024.2395565","DOIUrl":"10.1080/21623945.2024.2395565","url":null,"abstract":"Background: Obesity increases the risk of atrial fibrillation (AF). We hypothesize that 'obese' epicardial adipose tissue (EAT) is, regardless of comorbidities, associated with markers of AF vulnerability.Methods: Patients >40y of age undergoing bariatric surgery and using <2 antihypertensive drugs and no insulin were prospectively included. Study investigations were conducted before and 1y after surgery. Heart rhythm and p-wave duration were measured through ECGs and 7-d-holters. EAT-volume and attenuation were determined on non-enhanced CT scans. Serum markers were quantified by ELISA.Results: Thirty-seven patients underwent surgery (age: 52.1 ± 5.9y; 27 women; no AF). Increased p-wave duration correlated with higher BMI, larger EAT volumes, and lower EAT attenuations (p < 0.05). Post-surgery, p-wave duration decreased from 109 ± 11 to 102 ± 11ms. Concurrently, EAT volume decreased from 132 ± 49 to 87 ± 52ml, BMI from 43.2 ± 5.2 to 28.9 ± 4.6kg/m2, and EAT attenuation increased from -76.1 ± 4.0 to -71.7 ± 4.4HU (p <0.001). Adiponectin increased from 8.7 ± 0.8 to 14.2 ± 1.0 μg/ml (p <0.001). However, decreased p-wave durations were not related to changed EAT characteristics, BMI or adiponectin.Conclusion: In this explorative study, longer p-wave durations related to higher BMIs, larger EAT volume, and lower EAT attenuations. P-wave duration and EAT volume decreased, and EAT attenuation increased upon drastic weightloss. However, there was no relation between decreased p-wave duration and changed BMI or EAT characteristics.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"13 1","pages":"2395565"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11385166/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142152964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anaesthetics reduce the viability of adipose-derived stem cells. 麻醉剂会降低脂肪干细胞的活力。

IF 3.5 4区生物学

Adipocyte Pub Date : 2024-12-01 Epub Date: 2024-05-23 DOI: 10.1080/21623945.2024.2351870

Maria Bugajska-Liedtke, Nadia Fatyga, Aleksander Brzozowski, Anna Bajek, Małgorzata Maj

{"title":"Anaesthetics reduce the viability of adipose-derived stem cells.","authors":"Maria Bugajska-Liedtke, Nadia Fatyga, Aleksander Brzozowski, Anna Bajek, Małgorzata Maj","doi":"10.1080/21623945.2024.2351870","DOIUrl":"10.1080/21623945.2024.2351870","url":null,"abstract":"Adipose-derived stem cells (ADSCs) are characterized by their low immunogenicity and unique immunosuppressive properties, providing many opportunities for autologous transplantation in regenerative medicine and plastic surgery. These methods are characterized by low rejection rates and intense stimulation of tissue regeneration. However, procedures during which fat tissue is harvested occur under local anaesthesia. To better understand the effects and mechanisms of anaesthetic compounds in cosmetic and therapeutic procedures, the present study used a mixture of these compounds (0.1% epinephrine, 8.4% sodium bicarbonate, and 4% articaine) and examined their impact on a human adipose-derived stem cell line. The results showed anesthetics' negative, dose-dependent effect on cell viability and proliferation, especially during the first 24 h of incubation. After extending the exposure to 48 and 72 h of incubation, cells adapted to new culture conditions. In contrast, no significant changes were observed in immunophenotype, cell cycle progression, and apoptosis. The results obtained from this study provide information on the effect of the selected mixture of anaesthetics on the characteristics and function of ASC52telo cells. The undesirable changes in the metabolic activity of cells suggest the need to search for new drugs to harvest cells with unaltered properties and higher efficacy in aesthetic medicine treatments.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"13 1","pages":"2351870"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11123512/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141080272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and analysis of scaffold-free adipose spheroids. 开发和分析无支架脂肪球。

IF 3.5 4区生物学

Adipocyte Pub Date : 2024-12-01 Epub Date: 2024-06-12 DOI: 10.1080/21623945.2024.2347215

Jesse Liszewski, Aloysious Klingelhutz, Edward A Sander, James Ankrum

{"title":"Development and analysis of scaffold-free adipose spheroids.","authors":"Jesse Liszewski, Aloysious Klingelhutz, Edward A Sander, James Ankrum","doi":"10.1080/21623945.2024.2347215","DOIUrl":"10.1080/21623945.2024.2347215","url":null,"abstract":"Adipose tissue plays a crucial role in metabolic syndrome, autoimmune diseases, and many cancers. Because of adipose's role in so many aspects of human health, there is a critical need for in vitro models that replicate adipose architecture and function. Traditional monolayer models, despite their convenience, are limited, showing heterogeneity and functional differences compared to 3D models. While monolayer cultures struggle with detachment and inefficient differentiation, healthy adipocytes in 3D culture accumulate large lipid droplets, secrete adiponectin, and produce low levels of inflammatory cytokines. The shift from monolayer models to more complex 3D models aims to better replicate the physiology of healthy adipose tissue in culture. This study introduces a simple and accessible protocol for generating adipose organoids using a scaffold-free spheroid model. The method, utilizing either 96-well spheroid plates or agarose micromolds, demonstrates increased throughput, uniformity, and ease of handling compared to previous techniques. This protocol allows for diverse applications, including drug testing, toxin screening, tissue engineering, and co-culturing. The choice between the two methods depends on the experimental goals, with the 96-well plate providing individualized control and the micromold offering scale advantages. The outlined protocol covers isolation, expansion, and characterization of stromal vascular fraction cells, followed by detailed steps for spheroid formation and optional downstream analyses.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"13 1","pages":"2347215"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11174133/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141305076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leptin secreted by adipocytes promotes EMT transition and endometrial cancer progression via the JAK2/STAT3 signalling pathway 脂肪细胞分泌的瘦素通过 JAK2/STAT3 信号通路促进 EMT 转变和子宫内膜癌的进展

IF 3.3 4区生物学

Adipocyte Pub Date : 2023-12-13 DOI: 10.1080/21623945.2023.2293273

Lifan Shen, Chen Zhang, Kaiying Cui, Xin Liang, Genhai Zhu, Lan Hong

引用次数: 0

Inhibition of AHCY impedes proliferation and differentiation of mouse and human adipocyte progenitor cells 抑制 AHCY 会阻碍小鼠和人类脂肪细胞祖细胞的增殖和分化

IF 3.3 4区生物学

Adipocyte Pub Date : 2023-12-08 DOI: 10.1080/21623945.2023.2290218

Paula Boczki, Marco Colombo, Juliane Weiner, Inka Rapöhn, Martin Lacher, Wieland Kiess, Martha Hanschkow, Antje Körner, Kathrin Landgraf

引用次数: 0

An optimised protocol for the investigation of insulin signalling in a human cell culture model of adipogenesis. 胰岛素信号在脂肪生成的人类细胞培养模型中研究的优化方案。

IF 3.3 4区生物学

Adipocyte Pub Date : 2023-12-01 DOI: 10.1080/21623945.2023.2179339

Jonathan M Gamwell, Keanu Paphiti, Leanne Hodson, Fredrik Karpe, Katherine E Pinnick, Marijana Todorčević

{"title":"An optimised protocol for the investigation of insulin signalling in a human cell culture model of adipogenesis.","authors":"Jonathan M Gamwell, Keanu Paphiti, Leanne Hodson, Fredrik Karpe, Katherine E Pinnick, Marijana Todorčević","doi":"10.1080/21623945.2023.2179339","DOIUrl":"10.1080/21623945.2023.2179339","url":null,"abstract":"While there is no standardized protocol for the differentiation of human adipocytes in culture, common themes exist in the use of supra-physiological glucose and hormone concentrations, and an absence of exogenous fatty acids. These factors can have detrimental effects on some aspects of adipogenesis and adipocyte function. Here, we present methods for modifying the adipogenic differentiation protocol to overcome impaired glucose uptake and insulin signalling in human adipose-derived stem cell lines derived from the stromal vascular fraction of abdominal and gluteal subcutaneous adipose tissue. By reducing the length of exposure to adipogenic hormones, in combination with a physiological glucose concentration (5 mM), and the provision of exogenous fatty acids (reflecting typical dietary fatty acids), we were able to restore early insulin signalling events and glucose uptake, which were impaired by extended use of hormones and a high glucose concentration, respectively. Furthermore, the addition of exogenous fatty acids greatly increased the storage of triglycerides and removed the artificial demand to synthesize all fatty acids by de novo lipogenesis. Thus, modifying the adipogenic cocktail can enhance functional aspects of human adipocytes in vitro and is an important variable to consider prior to in vitro investigations into adipocyte biology.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"12 1","pages":"2179339"},"PeriodicalIF":3.3,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9980465/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9463287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An optimized method for Oil Red O staining with the salicylic acid ethanol solution. 水杨酸乙醇溶液对油红O染色的优化方法。

IF 3.5 4区生物学

Adipocyte Pub Date : 2023-12-01 DOI: 10.1080/21623945.2023.2179334

Junbao Du, Li Zhao, Quan Kang, Yun He, Yang Bi

引用次数: 0

Integrative bioinformatics analysis to screen key genes and signalling pathways related to ferroptosis in obesity. 综合生物信息学分析，筛选与肥胖脱铁症相关的关键基因和信号通路。

IF 3.3 4区生物学

Adipocyte Pub Date : 2023-12-01 Epub Date: 2023-10-25 DOI: 10.1080/21623945.2023.2264442

Ming-Ke Li, Chang Xing, Lan-Qing Ma

{"title":"Integrative bioinformatics analysis to screen key genes and signalling pathways related to ferroptosis in obesity.","authors":"Ming-Ke Li, Chang Xing, Lan-Qing Ma","doi":"10.1080/21623945.2023.2264442","DOIUrl":"10.1080/21623945.2023.2264442","url":null,"abstract":"Ferroptosis is closely associated with the development of disease in the body. However, there are few studies on ferroptosis-related genes (FRGs) in obesity. Therefore, key genes and signalling pathways related to ferroptosis in obesity were screened. Briefly, the RNA sequencing data of obesity and the non-obesity human samples and 259 FRGs were downloaded from GEO database and FerrDb database, respectively. The obesity-related module genes were firstly screened by weighted gene co-expression network analysis (WGCNA) and crossed with differentially expressed genes (DEGs) of obesity/normal samples and FRGs to obtain obesity-ferroptosis related (OFR) DEGs. Then, key genes were screened by PPI network. Next, the correlation of key genes and differential immune cells between obesity and normal samples were further explored by immune infiltration analysis. Finally, microRNA (miRNA)-messenger RNA (mRNA), transcription factor (TF)-mRNA networks and drug-gene interaction networks were constructed. As a result, 17 OFR DEGs were obtained, which mainly participated in processes such as lipid metabolism or adipocyte differentiation. The 4 key genes, STAT3, IL-6, PTGS2, and VEGFA, constituted the network. M2 macrophages, T cells CD8, mast cells activated, and T cells CD4 memory resting had significant differences between obesity and normal samples. Moreover, 51 miRNAs and 164 drugs were predicted for 4 key genes. All in all, this study has screened 4 FRGs, including IL-6, VEGFA, STAT3, and PTGS2, in obesity patients.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"12 1","pages":"2264442"},"PeriodicalIF":3.3,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ba/1f/KADI_12_2264442.PMC10601513.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50160338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0