Adipocyte最新文献_第3页

Comparative analysis of adipose tissue mitophagy and inflammatory markers in obesity and health. 肥胖与健康中脂肪组织自噬和炎症标志物的比较分析。

IF 3.1 4区生物学

Adipocyte Pub Date : 2025-12-01 Epub Date: 2025-12-03 DOI: 10.1080/21623945.2025.2596407

Shaghayegh Mohammadzadeh, Mitra Nourbakhsh, Parichehreh Yaghmaei, Atefeh Kashanizadeh

{"title":"Comparative analysis of adipose tissue mitophagy and inflammatory markers in obesity and health.","authors":"Shaghayegh Mohammadzadeh, Mitra Nourbakhsh, Parichehreh Yaghmaei, Atefeh Kashanizadeh","doi":"10.1080/21623945.2025.2596407","DOIUrl":"10.1080/21623945.2025.2596407","url":null,"abstract":"Obesity is associated with chronic inflammation and disruptions in cellular homeostasis, including impaired autophagy in adipose tissue. This study aimed to investigate the key mitophagy markers in the adipose tissue of individuals with obesity, compared to healthy controls. A total of 60 participants were enrolled, comprising 30 individuals with obesity and 30 healthy controls. Adipose tissue and peripheral blood samples were collected from all participants. Biochemical analyses included measurement of tumor necrosis factor-alpha (TNF-α), leptin, succinate dehydrogenase (SDH), and oxidative stress markers. Gene expression levels of mitophagy-related genes, PARK2, PINK1, and BNIP3L were assessed using quantitative real-time PCR. Additionally, immunohistochemistry was performed to evaluate BNIP3L protein levels in adipose tissue. Compared to the control group, individuals with obesity showed significantly elevated levels of TNF-α and SDH, along with evidence of oxidative stress. Moreover, the expression of PARK2, PINK1, and BNIP3L was significantly upregulated in the obesity group, suggesting increased mitophagy activity in adipose tissue. These findings indicate heightened inflammation and upregulation of mitophagy pathways in the adipose tissue of individuals with obesity. The upregulation of mitophagy-related genes seems to indicate a possible activation of mitophagy-associated pathways in the altered metabolic and inflammatory environment of obesity.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"14 1","pages":"2596407"},"PeriodicalIF":3.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12688221/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145666417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-obese potentiality of marine Topse (Polynemus paradiseus) fish oil by inhibiting the expression of SREBP-1c & promoting β-oxidation of fat through upregulating PPAR-α. 海洋Topse （Polynemus paradiseus）鱼油通过上调PPAR-α抑制SREBP-1c表达并促进脂肪β-氧化的抗肥胖潜力

IF 3.5 4区生物学

Adipocyte Pub Date : 2025-12-01 Epub Date: 2025-06-28 DOI: 10.1080/21623945.2025.2524640

Riya Kar, Pipika Das, Titli Panchali, Ananya Dutta, Manisha Phoujdar, Kuntal Ghosh, Shrabani Pradhan

{"title":"Anti-obese potentiality of marine Topse (Polynemus paradiseus) fish oil by inhibiting the expression of SREBP-1c & promoting β-oxidation of fat through upregulating PPAR-α.","authors":"Riya Kar, Pipika Das, Titli Panchali, Ananya Dutta, Manisha Phoujdar, Kuntal Ghosh, Shrabani Pradhan","doi":"10.1080/21623945.2025.2524640","DOIUrl":"10.1080/21623945.2025.2524640","url":null,"abstract":"Considering the adverse effects of marketed drugs, we isolated and analysed topse fish oil (FO) in this study for the first time and examined its effect on obesity. Topse, scientifically known as Polynemus paradiseus, is a common fish species found in the maritime environment of the West Bengal region. To explore the role of marine P. paradiseus FO in alleviating obesity-related metabolic disorders in vivo model. Twenty-four male BALB/c mice with a standard body weight of 18.2 ± 2.1 g were taken and randomly divided into four groups: control group (C), normal chow feeding; obese control (OC), high fat diet (HFD) feeding; Treatment I (T-I) and Treatment II (T-II) group received 200 mg and 400 mg crude oil/kg body weight/day by gavage along with HFD. Here, we examined the effects of P. paradiseus oil on white adipose tissue (WAT) weight, lipid profiles, blood glucose, and adipokine expression levels in the OC group compared to the treated groups to evaluate the anti-obesity effects of FO. Compared to the HFD-induced OC group, the treated obese mice group (T-I and T-II) showed a significant reduction in body weight, Body Mass Index (BMI), and serum lipid profiles following the application of FO. The FO-treated HFD-induced obese mice group showed a moderate reduction in obesity and inflammatory-related adipocytokines compared to the OC group. Topse FO was enhanced with a large amount of essential fatty acids (FAs) and it might be administered as a dietary supplement to prevent obesity.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"14 1","pages":"2524640"},"PeriodicalIF":3.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12218541/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144525975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fatty acid composition of lipid fractions in white- and brown-like adipocytes derived from human mesenchymal stem cells. 来源于人间充质干细胞的白色和棕色样脂肪细胞中脂质组分的脂肪酸组成。

IF 3.1 4区生物学

Adipocyte Pub Date : 2025-12-01 Epub Date: 2025-10-01 DOI: 10.1080/21623945.2025.2566481

Khadijeh Abbasi, Amir Mehdizadeh, Hamed Hamishehkar, Mohammad Nouri, Masoud Darabi

{"title":"Fatty acid composition of lipid fractions in white- and brown-like adipocytes derived from human mesenchymal stem cells.","authors":"Khadijeh Abbasi, Amir Mehdizadeh, Hamed Hamishehkar, Mohammad Nouri, Masoud Darabi","doi":"10.1080/21623945.2025.2566481","DOIUrl":"10.1080/21623945.2025.2566481","url":null,"abstract":"White and brown adipocytes differ markedly in lipid composition and metabolic function. White adipocytes primarily serve as energy storage depots, whereas brown adipocytes are mitochondria-rich and specialized for thermogenesis. However, the lipidomic profiles of white-like (WLAs) and brown-like adipocytes (BLAs) differentiated from human mesenchymal stem cells (MSCs) remain incompletely characterized. Human adipose-derived MSCs were differentiated into WLAs and BLAs. Lipid fractions were isolated and analysed by gas-liquid chromatography. Fatty acid composition data were used to calculate indices of stearoyl-CoA desaturase-1 (SCD1) activity, elongation, and ω6 synthesis. Compared to MSCs, BLAs showed consistently elevated oleate (≥4.2-fold) and stearate (≥2.3-fold), along with reduced palmitate (≤-20%) and linoleate (≤-28%) across phospholipid, triglyceride, and free fatty acid fractions. WLAs versus MSCs showed similar trends, with oleate increasing up to 15-fold and palmitate decreasing by 67-82% depending on the lipid class. SCD1 activity and elongation indices were elevated in WLAs (SCD1: up to 4.7-fold; elongation: up to 28-fold). The ω6 synthesis index was also increased in triglyceride and free fatty acid fractions of WLAs (≥3.3-fold), but markedly suppressed in BLAs (≤-88.7%). WLAs and BLAs differentiated from MSCs exhibit distinct lipid profiles and inferred enzymatic activity patterns, reflecting their respective capacities for lipid storage and metabolic flexibility. These findings provide a foundation for future translational research aimed at targeting adipose tissue in obesity and metabolic diseases.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"14 1","pages":"2566481"},"PeriodicalIF":3.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12498536/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Atoh8 expression inhibition promoted osteogenic differentiation of ADSCs and inhibited cell proliferation in vitro and rat bone defect models. Atoh8表达抑制促进ADSCs成骨分化，抑制体外及大鼠骨缺损模型细胞增殖。

IF 3.5 4区生物学

Adipocyte Pub Date : 2025-12-01 Epub Date: 2025-05-12 DOI: 10.1080/21623945.2025.2494089

Zian Yi, Shuang Song, Yuxin Bai, Guanhua Zhang, Yuxi Wang, Zijun Chen, Xuefeng Chen, Banglian Deng, Xiangdong Liu, Zuolin Jin

{"title":"Atoh8 expression inhibition promoted osteogenic differentiation of ADSCs and inhibited cell proliferation in vitro and rat bone defect models.","authors":"Zian Yi, Shuang Song, Yuxin Bai, Guanhua Zhang, Yuxi Wang, Zijun Chen, Xuefeng Chen, Banglian Deng, Xiangdong Liu, Zuolin Jin","doi":"10.1080/21623945.2025.2494089","DOIUrl":"10.1080/21623945.2025.2494089","url":null,"abstract":"Stem cell-based bone tissue engineering offers a promising approach for treating oral and cranio-maxillofacial bone defects. This study investigated the role of Atoh8, a key regulator in various cells, in the osteogenic potential of adipose-derived stem cells (ADSCs). ADSCs transfected with small interfering RNA (siRNA) targeting Atoh8 were evaluated for proliferation, migration, adhesion, and osteogenic capacity. In vivo, 20 SD rats were used to assess bone regeneration using Atoh8-knockdown ADSC sheets, with new bone formation quantified via micro-CT and histological analysis. Atoh8 knockdown in vitro reduced ADSC proliferation and migration but enhanced osteogenic differentiation and upregulation of osteogenic-related factors. This approach improved bone healing in rat defect models, accelerating repair both in vitro and in vivo. The findings underscore the clinical potential of ADSCs in bone tissue engineering and elucidate Atoh8's regulatory role in ADSC osteogenesis, providing a novel therapeutic strategy for enhancing bone regeneration through targeted modulation of stem cell differentiation pathways.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"14 1","pages":"2494089"},"PeriodicalIF":3.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12077435/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143961412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Crosstalk between microRNAs and autophagy in adipocyte differentiation: emerging therapeutic targets for obesity. 脂肪细胞分化中microrna与自噬之间的串扰：肥胖症的新治疗靶点。

IF 3.1 4区生物学

Adipocyte Pub Date : 2025-12-01 Epub Date: 2025-10-27 DOI: 10.1080/21623945.2025.2580741

Shan Gao

引用次数: 0

WDR4-mediate tRNA m7G modification to promote mitophagy and browning of white adipose tissue for ameliorating obesity in male mice. wdr4介导tRNA m7G修饰促进白色脂肪组织的线粒体自噬和褐化以改善雄性小鼠肥胖

IF 3.1 4区生物学

Adipocyte Pub Date : 2025-12-01 Epub Date: 2025-11-25 DOI: 10.1080/21623945.2025.2588888

Beisi Lin, Chaofan Wang, Yanling Yang, Zhigu Liu, Panwei Mu, Wen Xu, Yonghui Li

{"title":"WDR4-mediate tRNA m7G modification to promote mitophagy and browning of white adipose tissue for ameliorating obesity in male mice.","authors":"Beisi Lin, Chaofan Wang, Yanling Yang, Zhigu Liu, Panwei Mu, Wen Xu, Yonghui Li","doi":"10.1080/21623945.2025.2588888","DOIUrl":"10.1080/21623945.2025.2588888","url":null,"abstract":"Objective: Brown adipose tissue activation is a potential anti-obesity strategy. N7-methylguanosine (m7G) modification is a novel RNA epigenetic modification, but its role in adipose metabolism remains unexplored.Methods: Male mice were fed a high-fat diet (HFD), followed by PCR array screening. Gain-of-function experiments and TRAC-seq were employed to explore WDR4 function.Result: A Mouse Epigenetic Modification Enzymes PCR Array revealed that WDR4 expression showed the most pronounced downregulation in HFD mice. Overexpression of WDR4 in 3T3-L1 cells and primary adipocytes significantly increased UCP1 expression and suppressed lipid droplet formation, and enhanced mitophagy as evidenced by mitochondrial ultrastructure, autophagic vesicles, and LC3 expression. Suppression of mitophagy using 3-MA and bafilomycin A1 attenuated WDR4-induced adipocyte browning. WDR4 overexpression enhanced translational activity and reshaped the tRNA m7G methylome in 3T3-L1 adipocytes, specifically induced 38 unique tRNA m7G modification sites, and increasing cleavage scores of multiple tRNAs. GSE229240 dataset revealed that WDR4 mutation significantly reduced translation efficiency of 195 genes enriched in the TGF-β signalling , including BMP8B. Knockdown of BMP8B partially counteracted WDR4-mediated mitophagy.Conclusion: WDR4 promotes adipocyte browning by enhancing BMP8B translation through tRNA m7G modification, revealing a novel m7G epitranscriptomic mechanism with therapeutic potential for obesity.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"14 1","pages":"2588888"},"PeriodicalIF":3.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12667666/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145601683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Disrupted adipokine secretion and inflammatory responses in human adipocyte hypertrophy. 人脂肪细胞肥大中脂肪因子分泌中断和炎症反应。

IF 3.5 4区生物学

Adipocyte Pub Date : 2025-12-01 Epub Date: 2025-04-03 DOI: 10.1080/21623945.2025.2485927

Dan Gao, Chen Bing, Helen R Griffiths

引用次数: 0

Hematopoietic stem cell-derived adipocytes suppress leptin production, and attenuate ovariectomy-induced inhibition of physical activity and insulin sensitivity in female mice. 造血干细胞来源的脂肪细胞抑制瘦素的产生，并减弱卵巢切除引起的雌性小鼠身体活动和胰岛素敏感性的抑制。

IF 3.1 4区生物学

Adipocyte Pub Date : 2025-12-01 Epub Date: 2025-08-04 DOI: 10.1080/21623945.2025.2536813

Andrew E Libby, Timothy M Sullivan, Joanne K Maltzahn, Matthew R Jackman, Kathleen M Gavin, Paul S MacLean, Wendy M Kohrt, Susan M Majka, Dwight J Klemm

{"title":"Hematopoietic stem cell-derived adipocytes suppress leptin production, and attenuate ovariectomy-induced inhibition of physical activity and insulin sensitivity in female mice.","authors":"Andrew E Libby, Timothy M Sullivan, Joanne K Maltzahn, Matthew R Jackman, Kathleen M Gavin, Paul S MacLean, Wendy M Kohrt, Susan M Majka, Dwight J Klemm","doi":"10.1080/21623945.2025.2536813","DOIUrl":"10.1080/21623945.2025.2536813","url":null,"abstract":"A subpopulation of adipocytes in mice and humans is produced from haematopoietic stem cells rather than mesenchymal progenitors; the source of conventional white and brown/beige adipocytes. The abundance of these haematopoietic stem cell-derived adipocytes (HSCDAs) is elevated in female mice by ovariectomy (OVX) or oestrogen receptor alpha (ERα) knockdown, suggesting that they may be involved in the metabolic and inflammatory pathology that accompany the loss of oestrogen signalling. However, we previously demonstrated that ablation of HSCDAs elevated circulating leptin levels while suppressing physical activity and insulin sensitivity. Here, we tested the combined impact of OVX with and without HSCDA ablation. We discovered that HSCDA depletion plus OVX raised circulating leptin levels more than HSCDA depletion alone. Likewise, while HSCDA depletion or OVX alone inhibited physical activity and insulin responsiveness, their combination further suppressed these endpoints. Other physiologic endpoints were regulated by OVX alone. We conclude that HSCDAs play a role inthe maintenance of a subset of metabolic endpoints related to normal adipose tissue function, and their elevated production in models of female sex hormone suppression occurs to normalize these endpoints. The results highlight the ability of HSCDAs to target physical activity and insulin responsiveness, possibly by normalizing leptin production.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"14 1","pages":"2536813"},"PeriodicalIF":3.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12323437/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144783225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adipose tissue and androgens: the ins and outs. 脂肪组织和雄激素：来龙去脉。

IF 3.5 4区生物学

Adipocyte Pub Date : 2025-12-01 Epub Date: 2025-06-09 DOI: 10.1080/21623945.2025.2508885

Yiyue Jia, Mathilde Lacombe, Catherine Muller, Delphine Milhas

引用次数: 0

A nomogram based on radiomic features from peri-prostatic adipose tissue for predicting bone metastasis in first-time diagnosed prostate cancer patients. 基于前列腺周围脂肪组织放射学特征预测首次诊断前列腺癌患者骨转移的影像学研究。

IF 3.5 4区生物学

Adipocyte Pub Date : 2025-12-01 Epub Date: 2025-06-19 DOI: 10.1080/21623945.2025.2517583

Bohao Liu, Qian Cai, Xiao Zhao, Huabin Su, Zhengxu Lin, Jialin Wu, Xiaoyang Li, Weian Zhu, Chen Zou, Yun Luo

{"title":"A nomogram based on radiomic features from peri-prostatic adipose tissue for predicting bone metastasis in first-time diagnosed prostate cancer patients.","authors":"Bohao Liu, Qian Cai, Xiao Zhao, Huabin Su, Zhengxu Lin, Jialin Wu, Xiaoyang Li, Weian Zhu, Chen Zou, Yun Luo","doi":"10.1080/21623945.2025.2517583","DOIUrl":"10.1080/21623945.2025.2517583","url":null,"abstract":"Purpose: To evaluate a radiomics-based nomogram using peri-prostatic adipose tissue (PPAT) features for predicting bone metastasis (BM) in newly diagnosed prostate cancer (PCa) patients.Methods: A retrospective study of 151 PCa patients (October 2010-November 2022) was conducted. Radiomic features were extracted from axial T2-weighted MRI of PPAT, and normalized PPAT was calculated as the ratio of PPAT volume to prostate volume. A radiomics score (Radscore) was developed using logistic regression with 16 features selected via LASSO regression. Independent predictors identified through univariate and multivariate logistic regression were used to construct a nomogram. Predictive performance was assessed using ROC curves, and internal validation involved 1000 bootstrapped iterations.Results: The Radscore, based on 16 features, showed significant association with BM and outperformed normalized PPAT in predictive value. Independent predictors of BM included Radscore, alkaline phosphatase (ALP), and clinical N stage (cN). A nomogram integrating these factors demonstrated strong discrimination (C-index: 0.908; 95% CI: 0.851-0.966) and calibration, with consistent results in validation (C-index: 0.903; 95% CI: 0.897-0.916). Decision curve analysis confirmed its clinical utility.Conclusions: Radscore, cN, and ALP were identified as independent BM predictors. The developed nomogram enables accurate risk stratification and personalized BM predictions for newly diagnosed PCa patients.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"14 1","pages":"2517583"},"PeriodicalIF":3.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12184149/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0