Adipocyte最新文献_第3页

Ethnic disparities and its association between epicardial adipose tissue thickness and cardiometabolic parameters. 种族差异及其心外膜脂肪组织厚度与心脏代谢参数之间的关联。

IF 3.5 4区生物学

Adipocyte Pub Date : 2024-12-01 Epub Date: 2024-02-19 DOI: 10.1080/21623945.2024.2314032

Was Mohamed, K N Ishak, N Baharum, Naz Zainudin, Han Yin Lim, Mfm Noh, Waw Ahmad, H Zaman Huri, Asm Zuhdi, S Sukahri, K Govindaraju, A H Abd Jamil

{"title":"Ethnic disparities and its association between epicardial adipose tissue thickness and cardiometabolic parameters.","authors":"Was Mohamed, K N Ishak, N Baharum, Naz Zainudin, Han Yin Lim, Mfm Noh, Waw Ahmad, H Zaman Huri, Asm Zuhdi, S Sukahri, K Govindaraju, A H Abd Jamil","doi":"10.1080/21623945.2024.2314032","DOIUrl":"10.1080/21623945.2024.2314032","url":null,"abstract":"Excessive deposit of epicardial adipose tissue (EAT) were recently shown to be positively correlated with cardiovascular disease (CVD). This study aims to investigate the thickness of EAT and its association with the components of metabolic syndrome among multi-ethnic Malaysians with and without acute coronary syndrome (ACS). A total of 213 patients were recruited, with the thickness of EAT were quantified non-invasively using standard two-dimensional echocardiography. EAT thickness among the Malaysian population was prompted by several demographic factors and medical comorbidities, particularly T2DM and dyslipidaemia. ACS patients have significantly thicker EAT compared to those without ACS (4.1 mm vs 3.7 mm, p = 0.035). Interestingly, among all the races, Chinese had the thickest EAT distribution (4.6 mm vs 3.8 mm), with age (p = 0.04 vs p < 0.001), and overall diastolic blood pressure (p = 0.028) was also found to be associated with EAT thickness. Further study is warranted to investigate its role as a cardiovascular risk marker among Malaysians with ACS.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"13 1","pages":"2314032"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10877981/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139904773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibiting TRIM8 alleviates adipocyte inflammation and insulin resistance by regulating the DUSP14/MAPKs pathway. 抑制 TRIM8 可通过调节 DUSP14/MAPKs 通路缓解脂肪细胞炎症和胰岛素抵抗。

IF 3.5 4区生物学

Adipocyte Pub Date : 2024-12-01 Epub Date: 2024-07-22 DOI: 10.1080/21623945.2024.2381262

Mingxue Zhu, Junliang Pu, Ting Zhang, Huarui Shao, Rui Su, Chengyong Tang

{"title":"Inhibiting TRIM8 alleviates adipocyte inflammation and insulin resistance by regulating the DUSP14/MAPKs pathway.","authors":"Mingxue Zhu, Junliang Pu, Ting Zhang, Huarui Shao, Rui Su, Chengyong Tang","doi":"10.1080/21623945.2024.2381262","DOIUrl":"10.1080/21623945.2024.2381262","url":null,"abstract":"Obesity is a low-grade chronic inflammation induced by the pathological expansion of adipocytes which allows the development of obesity-associated metabolic diseases like type 2 diabetes mellitus (T2D) and non-alcoholic fatty liver disease (NAFLD). However, mechanisms regulating adipocyte inflammation remain poorly understood. Here, we observed that TRIM8 was upregulated in adipocyte inflammation and insulin resistance while DUSP14 was downregulated. TRIM8 deficiency and DUSP14 over-expression decreased the level of inflammatory cytokines, increased glucose uptake content, and improved insulin signalling transduction compared to LPS treatment alone. Conversely, silencing DUSP14 increased the expression of inflammatory cytokines. It decreased the glucose uptake content and the phosphorylation level of proteins involved in insulin signalling, further impairing insulin signalling and aggravating insulin resistance. Furthermore, The decreased level of inflammatory cytokines, increased glucose uptake, and improved insulin signalling transduction caused by TRIM8 deficiency were reversed by down-regulated DUSP14. Collectively, our findings revealed that TRIM8 can regulate adipocyte inflammation and insulin resistance by regulating the MAPKs pathway which is dependent on DUSP14.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"13 1","pages":"2381262"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11268219/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141747144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The early transition to cold-induced browning in mouse subcutaneous white adipose tissue (scWAT) involves proteins related to nerve remodeling, cytoskeleton, mitochondria, and immune cells.

IF 3.5 4区生物学

Adipocyte Pub Date : 2024-12-01 Epub Date: 2024-12-06 DOI: 10.1080/21623945.2024.2428938

Magdalena Blaszkiewicz, Cory P Johnson, Jake W Willows, Miranda L Gardner, Dylan R Taplin, Michael A Freitas, Kristy L Townsend

{"title":"The early transition to cold-induced browning in mouse subcutaneous white adipose tissue (scWAT) involves proteins related to nerve remodeling, cytoskeleton, mitochondria, and immune cells.","authors":"Magdalena Blaszkiewicz, Cory P Johnson, Jake W Willows, Miranda L Gardner, Dylan R Taplin, Michael A Freitas, Kristy L Townsend","doi":"10.1080/21623945.2024.2428938","DOIUrl":"10.1080/21623945.2024.2428938","url":null,"abstract":"White adipose tissue (WAT) is a dynamic organ capable of remodelling in response to metabolic state. For example, in response to stimuli such as cold exposure, WAT can develop inducible brown adipocytes ('browning') capable of non-shivering thermogenesis, through concurrent changes to mitochondrial content and function. This is aided by increased neurite outgrowth and angiogenesis across the tissue, providing the needed neurovascular supply for uncoupling protein 1 activation. While several RNA-sequencing studies have been performed in WAT, including newer single cell and single nuclei studies, little work has been done to investigate changes to the adipose proteome, particularly during dynamic periods of tissue remodelling such as cold stimulation. Here, we conducted a comprehensive proteomic analysis of inguinal subcutaneous (sc) WAT during the initial 'browning' period of 24 or 72hrs of cold exposure in mice. We identified four significant pathways impacted by cold stimulation that are involved in tissue remodelling, which included mitochondrial function and metabolism, cytoskeletal remodelling, the immune response, and the nervous system. Taken together, we found that early changes in the proteome of WAT with cold stimulation predicted later structural and functional changes in the tissue that are important for tissue and whole-body remodelling to meet energetic and metabolic needs.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"13 1","pages":"2428938"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11633174/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142783832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding temporal thermogenesis: coregulator selectivity and transcriptional control in brown and beige adipocytes. 解码时间性产热：棕色和米色脂肪细胞中核心调节器的选择性和转录控制。

IF 3.5 4区生物学

Adipocyte Pub Date : 2024-12-01 Epub Date: 2024-08-18 DOI: 10.1080/21623945.2024.2391511

Yong Geun Jeon, Sun Won Kim, Jae Bum Kim

{"title":"Decoding temporal thermogenesis: coregulator selectivity and transcriptional control in brown and beige adipocytes.","authors":"Yong Geun Jeon, Sun Won Kim, Jae Bum Kim","doi":"10.1080/21623945.2024.2391511","DOIUrl":"10.1080/21623945.2024.2391511","url":null,"abstract":"In mammals, brown adipose tissue (BAT) and beige adipocytes in white adipose tissue (WAT) play pivotal roles in maintaining body temperature and energy metabolism. In mice, BAT quickly stimulates thermogenesis by activating brown adipocytes upon cold exposure. In the presence of chronic cold stimuli, beige adipocytes are recruited in inguinal WAT to support heat generation. Accumulated evidence has shown that thermogenic execution of brown and beige adipocytes is regulated in a fat depot-specific manner. Recently, we have demonstrated that ubiquitin ligase ring finger protein 20 (RNF20) regulates brown and beige adipocyte thermogenesis through fat-depot-specific modulation. In BAT, RNF20 regulates transcription factor GA-binding protein alpha (GABPα), whereas in inguinal WAT, RNF20 potentiates transcriptional activity of peroxisome proliferator-activated receptor-gamma (PPARγ) through the degradation of nuclear corepressor 1 (NCoR1). This study proposes the molecular mechanisms by which co-regulator(s) selectively and temporally control transcription factors to coordinate adipose thermogenesis in a fat-depot-specific manner. In this Commentary, we provide molecular features of brown and beige adipocyte thermogenesis and discuss the underlying mechanisms of distinct thermogenic processes in two fat depots.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"13 1","pages":"2391511"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11340756/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141999266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The change of epicardial adipose tissue characteristics and vulnerability for atrial fibrillation upon drastic weight loss. 体重急剧下降时心外膜脂肪组织特征和心房颤动易感性的变化

IF 3.5 4区生物学

Adipocyte Pub Date : 2024-12-01 Epub Date: 2024-09-09 DOI: 10.1080/21623945.2024.2395565

Eva R Meulendijks, Carolina Janssen-Telders, Elise L Hulsman, Nick Lobe, Pietro Zappala, Marc M Terpstra, Robin Wesselink, Tim A C de Vries, Rushd F Al-Shama, Ruben N van Veen, Steve M M de Castro, Claire E E de Vries, Leontien M G Nijland, R Nils Planken, Sebastien P J Krul, Joris R de Groot

{"title":"The change of epicardial adipose tissue characteristics and vulnerability for atrial fibrillation upon drastic weight loss.","authors":"Eva R Meulendijks, Carolina Janssen-Telders, Elise L Hulsman, Nick Lobe, Pietro Zappala, Marc M Terpstra, Robin Wesselink, Tim A C de Vries, Rushd F Al-Shama, Ruben N van Veen, Steve M M de Castro, Claire E E de Vries, Leontien M G Nijland, R Nils Planken, Sebastien P J Krul, Joris R de Groot","doi":"10.1080/21623945.2024.2395565","DOIUrl":"10.1080/21623945.2024.2395565","url":null,"abstract":"Background: Obesity increases the risk of atrial fibrillation (AF). We hypothesize that 'obese' epicardial adipose tissue (EAT) is, regardless of comorbidities, associated with markers of AF vulnerability.Methods: Patients >40y of age undergoing bariatric surgery and using <2 antihypertensive drugs and no insulin were prospectively included. Study investigations were conducted before and 1y after surgery. Heart rhythm and p-wave duration were measured through ECGs and 7-d-holters. EAT-volume and attenuation were determined on non-enhanced CT scans. Serum markers were quantified by ELISA.Results: Thirty-seven patients underwent surgery (age: 52.1 ± 5.9y; 27 women; no AF). Increased p-wave duration correlated with higher BMI, larger EAT volumes, and lower EAT attenuations (p < 0.05). Post-surgery, p-wave duration decreased from 109 ± 11 to 102 ± 11ms. Concurrently, EAT volume decreased from 132 ± 49 to 87 ± 52ml, BMI from 43.2 ± 5.2 to 28.9 ± 4.6kg/m2, and EAT attenuation increased from -76.1 ± 4.0 to -71.7 ± 4.4HU (p <0.001). Adiponectin increased from 8.7 ± 0.8 to 14.2 ± 1.0 μg/ml (p <0.001). However, decreased p-wave durations were not related to changed EAT characteristics, BMI or adiponectin.Conclusion: In this explorative study, longer p-wave durations related to higher BMIs, larger EAT volume, and lower EAT attenuations. P-wave duration and EAT volume decreased, and EAT attenuation increased upon drastic weightloss. However, there was no relation between decreased p-wave duration and changed BMI or EAT characteristics.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"13 1","pages":"2395565"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11385166/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142152964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Defining the role of 2,2',4,4'-tetrabromodiphenyl ether in 3T3-L1 cellular differentiation by transcriptome sequencing analysis.

IF 3.5 4区生物学

Adipocyte Pub Date : 2024-12-01 Epub Date: 2024-12-07 DOI: 10.1080/21623945.2024.2430717

Zao-Ling Liu, Aerna Qiayimaerdan, Yong Fan, Shu-Rui Jiang, Zunire Tuerxuna, Meng-Lin Wang, Haiqiemuhan Abudureheman

{"title":"Defining the role of 2,2',4,4'-tetrabromodiphenyl ether in 3T3-L1 cellular differentiation by transcriptome sequencing analysis.","authors":"Zao-Ling Liu, Aerna Qiayimaerdan, Yong Fan, Shu-Rui Jiang, Zunire Tuerxuna, Meng-Lin Wang, Haiqiemuhan Abudureheman","doi":"10.1080/21623945.2024.2430717","DOIUrl":"10.1080/21623945.2024.2430717","url":null,"abstract":"This study aims to investigates the effect of 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) on the differentiation of 3T3-L1 cells and its mechanism of action. These 3T3-L1 cells were induced to differentiate in vitro using methylisobutylxanthine, dexamethasone, and insulin conditions, then exposed to either 1% DMSO as a control group or varying concentrations of BDE-47 (2.5 μM, 7.5 μM, 12.5 μM, 18.75 μM, and 25 μM). Oil red O staining showed that the absorbance value of the BDE-47 exposure groups was higher than that of the control group (p < 0.05). This study identified 722 common genes between the differentially expressed genes of each exposure group. Using Cytoscape 10 hub genes were identified as Actb, Cdk1, Myc, Ccnb1, Aurkb, Plk1, Aurka, Pparg, Kif11, and Casp3. Enrichment analysis data revealed that the effects of BDE-47 on 3T3-L1 cell differentiation were associated with the cell cycle, p53 signalling, and PPARγ pathways. The transcription factor genes, KAT2A, MAX, SIN3A, TBP, and EP300, were shown to be associated with the PPARγ pathway. The mRNA expression of PPARγ in each exposure group was higher than that in the control group (p < 0.05), and a bimodal distribution between PPARγ mRNA expression and BDE-47 dose was observed. These findings indicate that BDE-47 May activate the PPARγ pathway and mitotic pathway to regulate the cell cycle and induce adipocyte differentiation.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"13 1","pages":"2430717"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11633175/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142790888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research progress on the pathogenesis of multiple symmetrical lipomatosis.

IF 3.5 4区生物学

Adipocyte Pub Date : 2024-12-01 Epub Date: 2024-10-17 DOI: 10.1080/21623945.2024.2416681

Bo Hu, Ze Wang, Tengxiao Ma, Pengfei Fan, Lei Li

引用次数: 0

Development and analysis of scaffold-free adipose spheroids. 开发和分析无支架脂肪球。

IF 3.5 4区生物学

Adipocyte Pub Date : 2024-12-01 Epub Date: 2024-06-12 DOI: 10.1080/21623945.2024.2347215

Jesse Liszewski, Aloysious Klingelhutz, Edward A Sander, James Ankrum

{"title":"Development and analysis of scaffold-free adipose spheroids.","authors":"Jesse Liszewski, Aloysious Klingelhutz, Edward A Sander, James Ankrum","doi":"10.1080/21623945.2024.2347215","DOIUrl":"10.1080/21623945.2024.2347215","url":null,"abstract":"Adipose tissue plays a crucial role in metabolic syndrome, autoimmune diseases, and many cancers. Because of adipose's role in so many aspects of human health, there is a critical need for in vitro models that replicate adipose architecture and function. Traditional monolayer models, despite their convenience, are limited, showing heterogeneity and functional differences compared to 3D models. While monolayer cultures struggle with detachment and inefficient differentiation, healthy adipocytes in 3D culture accumulate large lipid droplets, secrete adiponectin, and produce low levels of inflammatory cytokines. The shift from monolayer models to more complex 3D models aims to better replicate the physiology of healthy adipose tissue in culture. This study introduces a simple and accessible protocol for generating adipose organoids using a scaffold-free spheroid model. The method, utilizing either 96-well spheroid plates or agarose micromolds, demonstrates increased throughput, uniformity, and ease of handling compared to previous techniques. This protocol allows for diverse applications, including drug testing, toxin screening, tissue engineering, and co-culturing. The choice between the two methods depends on the experimental goals, with the 96-well plate providing individualized control and the micromold offering scale advantages. The outlined protocol covers isolation, expansion, and characterization of stromal vascular fraction cells, followed by detailed steps for spheroid formation and optional downstream analyses.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"13 1","pages":"2347215"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11174133/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141305076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anaesthetics reduce the viability of adipose-derived stem cells. 麻醉剂会降低脂肪干细胞的活力。

IF 3.5 4区生物学

Adipocyte Pub Date : 2024-12-01 Epub Date: 2024-05-23 DOI: 10.1080/21623945.2024.2351870

Maria Bugajska-Liedtke, Nadia Fatyga, Aleksander Brzozowski, Anna Bajek, Małgorzata Maj

{"title":"Anaesthetics reduce the viability of adipose-derived stem cells.","authors":"Maria Bugajska-Liedtke, Nadia Fatyga, Aleksander Brzozowski, Anna Bajek, Małgorzata Maj","doi":"10.1080/21623945.2024.2351870","DOIUrl":"10.1080/21623945.2024.2351870","url":null,"abstract":"Adipose-derived stem cells (ADSCs) are characterized by their low immunogenicity and unique immunosuppressive properties, providing many opportunities for autologous transplantation in regenerative medicine and plastic surgery. These methods are characterized by low rejection rates and intense stimulation of tissue regeneration. However, procedures during which fat tissue is harvested occur under local anaesthesia. To better understand the effects and mechanisms of anaesthetic compounds in cosmetic and therapeutic procedures, the present study used a mixture of these compounds (0.1% epinephrine, 8.4% sodium bicarbonate, and 4% articaine) and examined their impact on a human adipose-derived stem cell line. The results showed anesthetics' negative, dose-dependent effect on cell viability and proliferation, especially during the first 24 h of incubation. After extending the exposure to 48 and 72 h of incubation, cells adapted to new culture conditions. In contrast, no significant changes were observed in immunophenotype, cell cycle progression, and apoptosis. The results obtained from this study provide information on the effect of the selected mixture of anaesthetics on the characteristics and function of ASC52telo cells. The undesirable changes in the metabolic activity of cells suggest the need to search for new drugs to harvest cells with unaltered properties and higher efficacy in aesthetic medicine treatments.","PeriodicalId":7226,"journal":{"name":"Adipocyte","volume":"13 1","pages":"2351870"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11123512/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141080272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leptin secreted by adipocytes promotes EMT transition and endometrial cancer progression via the JAK2/STAT3 signalling pathway 脂肪细胞分泌的瘦素通过 JAK2/STAT3 信号通路促进 EMT 转变和子宫内膜癌的进展

IF 3.3 4区生物学

Adipocyte Pub Date : 2023-12-13 DOI: 10.1080/21623945.2023.2293273

Lifan Shen, Chen Zhang, Kaiying Cui, Xin Liang, Genhai Zhu, Lan Hong

引用次数: 0