Adipogenic characterization of immortalized CD55⁺ progenitor cells from human white adipose tissue.

IF 3.5 4区生物学 Q2 ENDOCRINOLOGY & METABOLISM

Adipocyte Pub Date : 2025-12-01 Epub Date: 2025-06-05 DOI:10.1080/21623945.2023.2283213

Morgane Couchet, Hui Gao, Felix Klingelhuber, Jutta Jalkanen, Thais De Castro Barbosa, Muhmmad Omar-Hmeadi, Lucas Massier, Natalie Krahmer, Niklas Mejhert, Mikael Rydén

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引用次数: 0

Abstract

Background: Mature adipocytes are difficult to study ex vivo, prompting the use of human adipose progenitor cells (hAPCs). However, hAPCs undergo replicative senescence, limiting their utility in long-term studies.

Methods: We inserted human telomerase reverse transcriptase (TERT) into the AAVS1 locus of CD55+ hAPCs derived from abdominal subcutaneous adipose tissue, and characterized the cells before and after adipogenic differentiation.

Results: TERT-hAPCs retained proliferative and adipogenic capacities for over 80 passages, comparable to early-passage wild type hAPCs. Transcriptomic and proteomic analyses confirmed strong adipocyte gene expression. Functionally, TERT-hAPCs responded to insulin and lipolytic stimuli (isoprenaline, dibutyryl cAMP, TNF-α). They adapted well to both 2D and 3D cultures, with improved adipogenesis under spheroid conditions.

Conclusion: Immortalization of CD55+ hAPCs yields cells with stable proliferative and adipogenic capacity across passages. Being cryopreservable and suitable for both 2D and 3D cultures, TERT-hAPCs offer a reliable, reusable model system for adipocyte studies using cells with a consistent genetic background.

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人白色脂肪组织中永生化CD55+祖细胞的成脂特性。

背景:成熟脂肪细胞是出了名的难以离体研究，因此开发了替代的细胞培养系统。最常见的模型之一是人类脂肪祖细胞(hAPCs)。不幸的是，在长时间的培养条件下，这些细胞表现出复制性衰老，这限制了它们在机制研究中的应用。方法:将人端粒酶逆转录酶(TERT)敲入来自腹腔皮下脂肪组织的CD55+ hAPCs的AAVS1位点，并对细胞分化为脂肪细胞前后的细胞进行表征。结果:永生化的TERT-hAPCs保持了与早期传代野生型hAPCs相当的增殖和成脂能力。与此一致，我们的整合转录组学和蛋白质组学分析显示，与野生型hapc相比，tert - hapc表现出强大的脂肪细胞表达谱。脂质转换的功能分析证实了这一点，其中TERT-hAPCs对胰岛素和促脂解刺激(如异丙肾上腺素、二丁基cAMP和肿瘤坏死因子α)表现出明显的反应。此外，TERT-hAPCs可以很容易地在标准的2D和3d培养中培养，蛋白质组学分析显示球形培养条件可以促进脂肪形成。结论:通过描述性和功能性研究，我们证明了人CD55+ hAPCs的永生化是可行的，并且在多次传代中产生的细胞具有稳定的增殖和成脂能力。由于这些细胞是可低温保存的，它们比原代细胞提供了额外的优势，允许在具有相同遗传背景的2D和3D模型系统中重复研究。(234/250)。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Adipocyte Medicine-Histology

CiteScore

6.50

自引率

3.00%

发文量

审稿时长

32 weeks

期刊介绍： Adipocyte recognizes that the adipose tissue is the largest endocrine organ in the body, and explores the link between dysfunctional adipose tissue and the growing number of chronic diseases including diabetes, hypertension, cardiovascular disease and cancer. Historically, the primary function of the adipose tissue was limited to energy storage and thermoregulation. However, a plethora of research over the past 3 decades has recognized the dynamic role of the adipose tissue and its contribution to a variety of physiological processes including reproduction, angiogenesis, apoptosis, inflammation, blood pressure, coagulation, fibrinolysis, immunity and general metabolic homeostasis. The field of Adipose Tissue research has grown tremendously, and Adipocyte is the first international peer-reviewed journal of its kind providing a multi-disciplinary forum for research focusing exclusively on all aspects of adipose tissue physiology and pathophysiology. Adipocyte accepts high-profile submissions in basic, translational and clinical research.