Archives of Microbiology最新文献

{"title":"Comprehensive validation of bactericidal efficacy of umbelliferone against multidrug-resistant pathogens through multifaceted antimicrobial assays.","authors":"Neetesh Mandal, Gulshan Sen, Abhishek Pathak, Kuldeep Gauliya, Manish Kumar Manjhi, Devanshi Chandel Upadhyay, Mohammed Latif Khan, Chandrama Prakash Upadhyay","doi":"10.1007/s00203-025-04382-4","DOIUrl":"https://doi.org/10.1007/s00203-025-04382-4","url":null,"abstract":"","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 8","pages":"180"},"PeriodicalIF":2.3,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of a method for the rapid detection of carbapenem-resistant Pseudomonas aeruginosa based on bla_NDM with one-tube RPA-CRISPR/Cas12a visualization.","authors":"Yan Wang, Haitao Yang, Kun Wang, Wenjun Zhu, Xizhen Wang","doi":"10.1007/s00203-025-04373-5","DOIUrl":"https://doi.org/10.1007/s00203-025-04373-5","url":null,"abstract":"<p><p>Pseudomonas aeruginosa (PA), which is a common Gram-negative bacterium, can become carbapenem-resistant Pseudomonas aeruginosa (CRPA) upon the development of antibiotic resistance, making clinical treatment difficult. CRPA with antibiotic resistance genes (ARGs), such as bla_NDM and bla_KPC, is common in China. The development of tests for ARGs would facilitate the more rapid identification of CRPA in China. Isothermal amplification research has improved, but limitations remain, including a lack of specialized equipment, the difficulty of developing sophisticated primers, and aerosol pollution. Thus, clinical examination methods must improve. We successfully integrated RPA with CRISPR/Cas12a, and we identified bla_NDM as our institution's primary CRPA. RPA-CRISPR/Cas12a could accurately detect target DNA within 40 min without cross-reacting with other bacteria. The results showed high concordance with conventional culture-based methods, including 100% agreement in clinical sample validation. The method reliably identified standard PA strains and 29 clinical isolates, detecting PA at concentrations as low as 10^-1 CFU. In addition, the closed-tube format effectively minimized the risk of aerosol contamination. This platform offers a rapid and user-friendly tool for identifying bla_NDM-positive CRPA, and this tool is particularly suitable for early screening and clinical management in resource-limited settings. The simplicity and accuracy of this approach make it a promising option for infection control and public health surveillance.</p>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 8","pages":"179"},"PeriodicalIF":2.3,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Jatrorrhizine attenuates inflammatory response in Helicobacter pylori-induced gastritis by suppressing NLRP3 inflammasomes and NF-κB signaling pathway.","authors":"Fang Xie, Shiqing Yao","doi":"10.1007/s00203-025-04374-4","DOIUrl":"https://doi.org/10.1007/s00203-025-04374-4","url":null,"abstract":"<p><p>Helicobacter pylori (H. pylori) was demonstrated to induce gastric mucosa inflammation in chronic atrophic gastritis (CAG). Jatrorrhizine possesses numerous biological properties, including antibacterial and anti-inflammatory effects. The present study was aimed at investigating whether Jatrorrhizine inhibits H. pylori-induced CAG and elucidating the underlying potential mechanisms. The rats were orally inoculated with H. pylori to establish CAG model. Fourteen days after Jatrorrhizine treatment, we measured the rat's body weight as well as rat's food intake. Besides, alcian blue and periodic acid-Schiff (AB-PAS) staining and hematoxylin-eosin (HE) staining were performed to observe the hisological changes of gastric mucosa. The inflammatory cytokines and gastric mucosa factors were measured by ELISA. The protein level in gastric mucosa was detected by Western blotting. H. pylori inoculation significantly induced gastric dysfunction, inflammatory infiltration and gastric mucosa injury, suggesting CAG rat model was successfully constructed. Nonetheless, Jatrorrhizine treatment effectively improved gastric function and pathological injury. Besides, Jatrorrhizine suppressed the inflammation and colonization of H. pylori in CAG rats. Moreover, Jatrorrhizine administration not only decreased inflammatory cytokines but also increased gastric mucosa factors, implying its protective effect on gastric mucosal. Mechanistically, Jatrorrhizine restrained the activation of NLRP3 inflammasomes and NF-κB signaling. Jatrorrhizine had inhibitory effects on H. pylori-induced inflammation, thus suppressing the progression of CAG via inactivation of NF-κB/NLRP3 signaling. Our findings underscore the potential of Jatrorrhizine as a promising candidate for clinical trials for CAF treatment.</p>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 8","pages":"177"},"PeriodicalIF":2.3,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144315838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing the power: the role of dissimilatory metal-reducing bacteria in microbial fuel cells.","authors":"Soumyadeep Chakraborty, Soumyajit Chandra, Soumya Pandit, Swetha Raj, Harjot Singh Gill, Kuldeep Sharma, Debasmita Bhattacharya, Moupriya Nag, Dibyajit Lahiri","doi":"10.1007/s00203-025-04319-x","DOIUrl":"https://doi.org/10.1007/s00203-025-04319-x","url":null,"abstract":"<p><p>Dissimilatory metal-reducing bacteria (DMRB) have been considered very important contributors in developing and operating microbial fuel cells that represent one promising technology for waste treatment and sustainable energy generation. In keeping with this spirit, this review paper will scrutinise the elementary mechanisms whereby the unique metabolic processes of DMRB enable their role in facilitating the extracellular transmission of electrons to the anode from organic substrates. Important species like Shewanella and Geobacter are referred to because of their contributions toward improving the stability and efficiency of MFCs. The paper also discusses the benefits of using DMRB, such as their potential in bioremediation and increased electron transfer efficiency. Difficulties examined include preserving microbial stability, competing with other species, and improving operating conditions. The recent developments in materials science, genetic engineering, and integration with other renewable technologies are discussed to demonstrate the potential for future breakthroughs. The last section of this paper discusses the wider implications of DMRB in developing MFC technology for energy and environmental applications.</p>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 8","pages":"176"},"PeriodicalIF":2.3,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144315837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rising trend in the microbial fermentation for succinic acid production: a comprehensive overview on innovative approaches using versatile biological sources.","authors":"Vignesh Natarajan","doi":"10.1007/s00203-025-04383-3","DOIUrl":"https://doi.org/10.1007/s00203-025-04383-3","url":null,"abstract":"<p><p>Succinic acid (SA) necessitates thorough examination regarding its biological production pathway. The energy-dependent biosynthesis of SA and the resulting intracellular redox imbalance contribute to reduced SA productivity. Co-culture fermentation that combines aerobic yeast and facultative bacteria demonstrates a dual benefit for microbial growth and SA production. The optimization of the fermentation process with native SA producers resulted in higher SA titer values. The intermittent addition of bicarbonate salts increased SA production to 3.86 g L⁻¹ h⁻¹ during the anaerobic fermentation of Actinobacillus succinogenes. Fed-batch fermentation of acetic acid enhanced SA production to 12 g/L via a glyoxylate shunt in Yarrowia lipolytica. The immobilization of SA-producing microbial strains enhanced continuous fermentation, resulting in SA titers and productivity of 69 g/L and 35.6 g L⁻¹ h⁻¹, respectively. Metabolic enhancements of SA yield are increasingly documented through various rational engineering approaches applied to bacterial and yeast strains. This review paper aims to analyze the challenges associated with conventional SA fermentation processes. This work examines literature on SA production from lignocellulosic biomass, offering a comprehensive analysis of the valorization of complex biological resources. This paper emphasizes recent advancements in SA yields, reaching nearly 0.98 g SA/g substrate, attained through innovative methodologies.</p>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 8","pages":"178"},"PeriodicalIF":2.3,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144315839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Opacimonas immobilis sp. nov., a novel non-flagellated Alteromonadaceae species with the smallest genome isolated from the phycosphere of the diatom Actinocyclus curvatulus.","authors":"Xiaoyu Yang, Xuanru Lin, Runlin Cai, Hui Wang, Guanjing Cai","doi":"10.1007/s00203-025-04375-3","DOIUrl":"https://doi.org/10.1007/s00203-025-04375-3","url":null,"abstract":"<p><p>A bacterial strain LMIT016^T, characterized as Gram-stain-negative, oval shaped, aerobic and non-motile, was isolated from the phycosphere of the diatom Actinocyclus curvatulus. Both the phylogenetic tree constructed from 16S rRNA gene sequences and the phylogenomic analysis using GTDB-Tk revealed that strain LMIT016^T was closely related to O. viscosa LMIT007^T (96.08% of 16S rRNA gene sequence identity). Strain LMIT016^T and related type species in the family exhibited average nucleotide identity values ranging from 76.37 to 79.96%, with digital DNA-DNA hybridization values below 30.0%, confirming that they represent different species. Notably, strain LMIT016^T possesses the smallest genome (2.53 Mbp) among Alteromonadaceae family, completely lacking genes related to flagellar assembly and chemotaxis. However, comparative genomic analysis revealed that it kept adaptive functions for phycosphere lifestyle, such as biofilm formation, carbohydrate metabolism, and vitamin and carotenoid synthesis. Further, strain LMIT016^T exhibited optimal growth under conditions of 1.0-5.0% NaCl, pH 6-10, and temperatures between 20 and 35 ℃. The major respiratory quinone was ubiquinone-8. The major fatty acids (> 10%) were identified as C_16:1ω7c/C_16:1ω6c (32.4%), C_16:0 (17.3%) and C_18:1ω7c/C_18:1ω6c (31.0%), while the major polar lipids comprised phosphatidylethanolamine, aminophospholipid and phosphatidylglycerol. These findings, combined with the phylogenetic and genotypic features, confirmed that strain LMIT016^T represented a novel species in the genus Opacimonas, for which the name Opacimonas immobilis sp. nov. was proposed, with type strain LMIT016^T (= KCTC 92596^T = MCCC 1K08254^T). The identification of this strain enriches the understanding of microbial diversity within the genus Opacimonas and highlights the specialized bacterial evolution for inhabiting the phycosphere.</p>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 8","pages":"173"},"PeriodicalIF":2.3,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144282281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential role of plant-derived thymol and carvacrol against Newcastle disease virus in poultry.","authors":"Arslan Muhammad Ali Khan, Silla Ambrose, Rao Zahid Abbas","doi":"10.1007/s00203-025-04370-8","DOIUrl":"https://doi.org/10.1007/s00203-025-04370-8","url":null,"abstract":"<p><p>Newcastle disease virus (NDV) is a serious danger to the world's poultry industry and can result in huge financial losses. Emerging virus strains and vaccination failures pose a threat to traditional control measures like vaccination, which is why other approaches must be investigated. The antiviral qualities of thymol and carvacrol, two important constituents of essential oils (EOs), are discussed in this review as a potential strategy to counteract NDV. These substances, which are isolated from a variety of aromatic plants, have a wide range of biological properties, such as immunomodulatory, antioxidant, and antiviral properties. Through a variety of methods, like breakdown of the viral envelope and suppression of viral entry and replication, thymol and carvacrol prevent viruses from replicating. The effectiveness of NDV control is increased by its capacity to alter the host immunological response. The extraction processes of these substances are compiled in this review, with a focus on cutting-edge approaches such as supercritical fluid extraction, which provide more sustainability and efficiency. Numerous investigations have demonstrated antiviral actions of carvacrol and thymol against NDV, highlighting their potential as natural substitutes for traditional antiviral treatments. Even though these EOs compounds show encouraging outcomes, further study is required to maximize their use in poultry.</p>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 8","pages":"175"},"PeriodicalIF":2.3,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144282282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical characterization of lipase from Cryptococcus albidus (D24) and its application in sugar fatty acid ester synthesis.","authors":"Duygu Elif Yilmaz, Ceyda Kula, Hasan Demirci","doi":"10.1007/s00203-025-04369-1","DOIUrl":"10.1007/s00203-025-04369-1","url":null,"abstract":"<p><p>Microbial lipases are widely used in industrial applications. The lipase enzyme from Cryptococcus albidus (D24) strain was biochemically characterized and evaluated for its potential in catalyzing esterification reactions, particularly in the synthesis of sugar fatty acid esters. The enzyme demonstrated enhanced activity in solvents like acetone, isopropanol, and dimethylformamide. The effects of different metal ions on the stability of the enzyme were also evaluated, and the results revealed an increased activity with Mn²⁺, K⁺ and Co²⁺. The molecular weight of the D24 lipase was determined to be 36.31 kDa, placing it within the range of other yeast-originated lipases. The kinetic parameters, including Km and Vmax, were calculated to be 1.58 × 10^-4 mM p-nitrophenyl palmitate (pNPP) and 26.31 U/min respectively, according to the Lineweaver-Burk plot. The enzyme exhibited promising results in catalyzing the esterification reaction to yield L-proline-glucose ester, as well as the synthesis of fructose monopalmitate. Thin layer chromatography (TLC) analysis of the lipase-catalyzed synthesis of fructose monopalmitate showed that, from 24 to 40 h, fructose monopalmitate concentration increased from 4.5 to 8.4% (w/w of the reaction mixture). These findings suggest the potential of D24 lipase for applications in synthesis of industrial products. This study is the first to report on the biochemical properties of the D24 lipase, offering a novel and sustainable enzymatic alternative for the future biotechnological applications.</p>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 8","pages":"174"},"PeriodicalIF":2.3,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12165992/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144282280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cnb proteins affect the intracellular 2-MIB retention in heterologous gene expression systems.","authors":"Kaushalya Dayarathne, Kimie Atsuzawa, Toshiki Ishikawa, Satoru Watanabe, Yasuko Kaneko, Aikeranmu Kadeer, Masatoshi Yamaguchi, Maki Kawai-Yamada","doi":"10.1007/s00203-025-04377-1","DOIUrl":"10.1007/s00203-025-04377-1","url":null,"abstract":"","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 8","pages":"172"},"PeriodicalIF":2.3,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12159092/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144265171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring thurincin H as a biotechnologically relevant sactipeptide after fifteen years of research.","authors":"Daniela Ruíz-De-Anda, María Fernanda Mendoza-Acosta, Luz Edith Casados-Vázquez","doi":"10.1007/s00203-025-04372-6","DOIUrl":"https://doi.org/10.1007/s00203-025-04372-6","url":null,"abstract":"<p><p>Thurincin H is an anionic antimicrobial peptide of 31 amino acids with a molecular weight of 3139.51 Da, it belongs to the group of sactipeptides and is produced by the bacterium Bacillus thuringiensis. Its antimicrobial spectrum is mainly directed against Gram-positive bacteria, and it has been shown to have antimicrobial activity against food pathogens and phytopathogens. In addition, it is attributed with the ability to act as a signaling molecule that promotes growth in plants, especially under stress conditions. Therefore, it is considered a peptide with biotechnological potential. One of the main limitations for the biotechnological use of thurincin H is its low production yield, mainly due to the complexity of its biosynthesis, which also hampers heterologous expression. To address this, several studies have investigated the functions of genes within its biosynthetic cluster. This review aims to summarize the key discoveries and research findings on thurincin H and explore its potential applications in biotechnology.</p>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 8","pages":"171"},"PeriodicalIF":2.3,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144265172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}