Archives of Microbiology最新文献

Ecological niche and risk modeling of West Nile virus using environmental and biological thresholds. 利用环境和生物阈值建立西尼罗病毒生态位和风险模型。

IF 2.6 3区生物学

Archives of Microbiology Pub Date : 2025-10-06 DOI: 10.1007/s00203-025-04488-9

Ismahane Nabti, Soumeya Chahed

引用次数: 0

A review on the mechanisms and applications of bacteria in responding, adsorbing and transporting heavy metals. 综述了细菌对重金属的响应、吸附和转运机理及其应用。

IF 2.6 3区生物学

Archives of Microbiology Pub Date : 2025-10-06 DOI: 10.1007/s00203-025-04441-w

Shangchen Sun, Miao Zhang, Guangrui Yang, Yonggang Wang, Zhen Chen

{"title":"A review on the mechanisms and applications of bacteria in responding, adsorbing and transporting heavy metals.","authors":"Shangchen Sun, Miao Zhang, Guangrui Yang, Yonggang Wang, Zhen Chen","doi":"10.1007/s00203-025-04441-w","DOIUrl":"https://doi.org/10.1007/s00203-025-04441-w","url":null,"abstract":"Bioremediation of heavy metal-contaminated sites is currently considered one of the most promising strategies for environmental remediation. Bacteria, with their simple structure and ease of modification, serve as ideal biological resources. Although numerous review studies have examined the mechanisms of microbial adsorption of heavy metals, most focus on macro-level processes. However, there is a lack of systematic research on how microorganisms respond to heavy metal stress and how their cellular components and metabolites in the adsorption and transport of heavy metals. In this review, we collected scientific information on bacterial-mediated bioremediation of heavy metals and conducted a comprehensive induction and analysis of it in combination with our own insights. The results showed that due to their structural composition, bacterial cell walls and membranes serve as barriers against heavy metal stress. Bacterial cells achieve heavy metal adsorption, detoxification, and transport through extracellular adsorption, intracellular chelation, and efflux. Specifically, extracellular adsorption involves ion exchange, complexation, redox reactions, nanoparticle formation, and biomineralization. Intracellular chelation is primarily mediated by bacterial metabolites such as metallothioneins and siderophores, while transport is mainly facilitated by components of efflux systems, including P-ATPases, CDF family proteins, and CBA transporters. This review aims to clarify patterns of organism-environment interactions, advance the development and application of heavy metal biosorbents, and further mitigate the risks of heavy metals to humans and the environment.","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 11","pages":"286"},"PeriodicalIF":2.6,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145231462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dark septate endophytic fungus Alternaria sp. 17463 regulates antioxidant enzymes and metabolism in response to acid-base stress. 暗隔内生真菌Alternaria sp. 17463调节抗氧化酶和代谢对酸碱胁迫的响应。

IF 2.6 3区生物学

Archives of Microbiology Pub Date : 2025-10-06 DOI: 10.1007/s00203-025-04492-z

Yinli Bi, Jing Zhao, Hai Tan, Shishuang Zhang

引用次数: 0

Enzymatic and biosurfactant production with antimicrobial activity by Bacillus subtilis group. 枯草芽孢杆菌群生产具有抗菌活性的酶和生物表面活性剂。

IF 2.6 3区生物学

Archives of Microbiology Pub Date : 2025-10-06 DOI: 10.1007/s00203-025-04486-x

Mariana Prósperi de Oliveira Paula, Clara Resende de Souza Castro, Juliete Gomes de Lara, Luciana Silva Ribeiro, Victor Satler Pylro, Cristina Ferreira Silva

{"title":"Enzymatic and biosurfactant production with antimicrobial activity by Bacillus subtilis group.","authors":"Mariana Prósperi de Oliveira Paula, Clara Resende de Souza Castro, Juliete Gomes de Lara, Luciana Silva Ribeiro, Victor Satler Pylro, Cristina Ferreira Silva","doi":"10.1007/s00203-025-04486-x","DOIUrl":"https://doi.org/10.1007/s00203-025-04486-x","url":null,"abstract":"Biosurfactants are valuable microbial metabolites with broad industrial and environmental applications. In this study, we investigated biosurfactant production by Bacillus subtilis group strains using residual glycerol as the sole carbon source, while also evaluating their enzymatic activity and antimicrobial properties. PCR-based gene detection confirmed the presence of genes encoding surfactin, fengycin, and iturin peptide biosynthesis. Crude glycerol, a byproduct of biodiesel production, supported biosurfactant synthesis by the tested strains. Strains CCMA 0087, 0658, and 2031 exhibited diesel oil emulsification rates ranging from approximately 53 to 61%, while also reducing water surface tension to around 30 mN/m. Notably, the crude biosurfactant produced by Bacillus velezensis CCMA 0087 inhibited Escherichia coli growth, whereas Bacillus subtilis CCMA 2031 partially inhibited Fusarium paranaense (~ 38%), representing the first report of biosurfactant activity against this phytopathogen. Ligninolytic enzyme assays revealed that strain 2031 exhibited the highest manganese peroxidase (MnP) activity, while 0087 showed the greatest lignin peroxidase (LiP) activity. These findings highlight the potential of biosurfactants and ligninolytic enzymes from Bacillus subtilis group strains for biocontrol and bioremediation applications.","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 11","pages":"287"},"PeriodicalIF":2.6,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145231475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Paracoccus sp. PS1: a novel probiotic strain for the mitigation of cardiometabolic risk factors TMA, TMAO, and methylglyoxal. 副球菌sp. PS1：一种减轻心脏代谢危险因素TMA、TMAO和甲基乙二醛的新型益生菌菌株。

IF 2.6 3区生物学

Archives of Microbiology Pub Date : 2025-10-06 DOI: 10.1007/s00203-025-04493-y

Madhupa Seth, Mahamadul Mondal, Subhra Kanti Mukhopadhyay

{"title":"Paracoccus sp. PS1: a novel probiotic strain for the mitigation of cardiometabolic risk factors TMA, TMAO, and methylglyoxal.","authors":"Madhupa Seth, Mahamadul Mondal, Subhra Kanti Mukhopadhyay","doi":"10.1007/s00203-025-04493-y","DOIUrl":"https://doi.org/10.1007/s00203-025-04493-y","url":null,"abstract":"The gut microbiome is increasingly recognized as a critical modulator of host health, particularly in the context of non-communicable diseases like cardiovascular disease and diabetes. Central to this connection are microbially-derived metabolites such as trimethylamine N-oxide (TMAO), trimethylamine (TMA), and methylglyoxal, which have emerged as significant biomarkers and mediators of disease progression. All these toxic metabolites are generated in the body by the type of food we consume on daily basis. As the epicentre of TMA mediated ailments is human gut, a probiotic microbial strain with TMA degrading ability may be useful in mitigating those ailments. Due to its strong TMA-degrading ability and non-pathogenic nature of our isolated strain Paracoccus sp. PS1 prompted further investigation of its other physio-biochemical properties to judge its suitability as a probiotic strain. With this aim, viability of PS1 under anaerobic and microaerophilic conditions, which mimic human gut, were examined. Strain PS1 showed positive growth under both microaerophilic and anaerobic conditions and also showed other probiotic features such as tolerance to bile, lysozyme, acidic pH, gastric juices (pepsin), hydrophobicity, aggregation, coaggregation, adhesion and antioxidant properties. Furthermore, analysis of the whole genome sequence of Paracoccus sp. PS1 identified the genes and their respective proteins responsible for its probiotic properties, supporting its potential for use as a novel probiotic strain. The present study is the first to identify and characterize a potential probiotic from the genus Paracoccus with the unique capacity to degrade TMA, TMAO, and methylglyoxal.","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 11","pages":"285"},"PeriodicalIF":2.6,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145231437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A BCG-boosting subunit vaccine RS2/DMI induces Th1-polarized immunity by targeting RD6 and secretory antigens of Mycobacterium tuberculosis. 一种bcg增强亚单位疫苗RS2/DMI通过靶向RD6和结核分枝杆菌分泌抗原诱导th1极化免疫。

IF 2.6 3区生物学

Archives of Microbiology Pub Date : 2025-10-06 DOI: 10.1007/s00203-025-04481-2

Xiaochun Wang, Qiangsen Zhong, Yun Xu, Xinkuang Liu

{"title":"A BCG-boosting subunit vaccine RS2/DMI induces Th1-polarized immunity by targeting RD6 and secretory antigens of Mycobacterium tuberculosis.","authors":"Xiaochun Wang, Qiangsen Zhong, Yun Xu, Xinkuang Liu","doi":"10.1007/s00203-025-04481-2","DOIUrl":"https://doi.org/10.1007/s00203-025-04481-2","url":null,"abstract":"Bacillus Calmette-Guérin (BCG) vaccine has limited protection against tuberculosis (TB) in adults, and there is an urgent need to develop novel TB vaccines. Currently, booster vaccines based on initial immunization with BCG are important research hotspots. In this study, we constructed recombinant protein RS2 by innovatively fusing Mycobacterium tuberculosis (M. tb) region of difference (RD) antigen Rv1506c with secretory phase antigen Rv3204, and developed a novel composite adjuvant DMI (dimethyl dioctadecylammonium bromide [DDA] + colloidal manganese salt [Mn jelly, MnJ] + imiquimod) to prepare a novel TB subunit vaccine, RS2/DMI. Population experiments showed that RS2 efficiently activated Th1-type cytokine secretion (IFN-γ/TNF-α/IL-2) in infected (active/ latent) individuals; in a mouse model, RS2/DMI as a BCG booster vaccine induced the highest level of IgG antibody response and the strongest Th1-polarized immune response - including the highest levels of Th1-type cytokines (IFN-γ/TNF-α /IL-2) and IFN-γ⁺IL-2⁺ double-positive CD4⁺/CD8⁺ T-cell production, and significantly better immunogenicity than the other adjuvant combinations (RS2/DMV, RS2/DIV) and BCG-immunized groups (P < 0.01). This immunological strategy also demonstrated matched preventive efficacy in ex vivo challenge experiment, confirming its potential as an efficient BCG booster vaccine and providing a new strategy to overcome the bottleneck of TB protection in adults.","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 11","pages":"290"},"PeriodicalIF":2.6,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145231429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

α-glucosidase inhibitory potential of endophytic Colletotrichum fructicola of Cinnamomum tamala. 柽柳肉桂内生炭疽菌α-葡萄糖苷酶抑制电位的研究。

IF 2.6 3区生物学

Archives of Microbiology Pub Date : 2025-10-06 DOI: 10.1007/s00203-025-04459-0

Ankita Arora, Sanjai Saxena

{"title":"α-glucosidase inhibitory potential of endophytic Colletotrichum fructicola of Cinnamomum tamala.","authors":"Ankita Arora, Sanjai Saxena","doi":"10.1007/s00203-025-04459-0","DOIUrl":"https://doi.org/10.1007/s00203-025-04459-0","url":null,"abstract":"Despite the antidiabetic significance of Cinnamomum tamala (Buch.-Ham.) T.Nees & C.H.Eberm., its endophytic fungal community remains largely unexplored. This study presents the first comprehensive investigation on endophytic fungal diversity of C. tamala, identifying fungi from 17 distinct genera colonizing its leaf, bark, and stem tissues. Moreover, this is the first report evaluating the α-glucosidase inhibitory potential of its endophytic fungi. A novel, cost-effective, and high-throughput qualitative spot assay was developed for preliminary screening. Among 87 fungal isolates screened, 32.2% exhibited α-glucosidase inhibitory activity, with isolate #2-4CTChP2-6 demonstrating the highest inhibition (IC50- 55.2 µg/mL). Morphological and multi-locus phylogenetic analysis identified this isolate as Colletotrichum fructicola. This is the first report of α-glucosidase inhibitory activity by C. fructicola from C. tamala. Optimization of culture conditions using a two-phase sequential approach: one-variable-at-a-time (OVAT) followed by Response Surface Methodology (RSM), enhanced activity by 1.27-fold, lowering the IC50 to 43.6 µg/mL. These findings position C. tamala-associated endophytes, particularly C. fructicola, as a promising microbial source of α-glucosidase inhibitors with potential relevance in the management of postprandial hyperglycemia.","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 11","pages":"284"},"PeriodicalIF":2.6,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145231465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flow cytometric quantification of methanogens in anaerobic digestate 厌氧消化液中产甲烷菌的流式细胞术定量

IF 2.6 3区生物学

Archives of Microbiology Pub Date : 2025-09-27 DOI: 10.1007/s00203-025-04468-z

Aatiya Shaikh, Srikanth Mutnuri

{"title":"Flow cytometric quantification of methanogens in anaerobic digestate","authors":"Aatiya Shaikh, Srikanth Mutnuri","doi":"10.1007/s00203-025-04468-z","DOIUrl":"10.1007/s00203-025-04468-z","url":null,"abstract":"<div>Anaerobic digestion (AD) is an efficacious waste treatment technique that promotes organic stability and energy recovery. Methanogens are critical organisms that constitute the bottleneck in this process. The process relies on methanogenic archaea, which are crucial organisms that create a bottleneck. The quantitative assessment of these organisms can enhance process efficiency, identify issues prior to failure and result in community-focused process control systems. This paper introduces a flow cytometric method that is autofluorescence-based and used to rapidly and economically measure methanogenic archaea in a selection of microbial communities and raw substrates. The technique was applied on a sample of anaerobic digestate. The methanogenic archaea were quantified by measuring the specific fluorescence of their co-factor F420 which were estimated as 1.85 × 105 cells mL^-1 using a standard curve with fluorescence intensity recorded throughout a 15-day period under storage in Phosphate Buffered Saline (PBS) at 6 °C. The thiazole orange screening facilitated the distinction of the complete microbial community from the persistent particle noise. The Sanger sequencing of the mcrA gene confirmed the findings leading to the detection of Methanosarcina sp. and Methanoculleus in FACS (Fluorescence-Activated Cell Sorting) sorted cells. This work presents new perspectives towards setting up of an anaerobic reactor that is exclusively based on methanogens thereby enhancing process efficiency and enabling the effective treatment of waste, hence reducing its environmental impact.</div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 11","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145169764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comprehensive review of advanced strategies to combat antimicrobial resistance 全面审查抗微生物药物耐药性的先进战略。

IF 2.6 3区生物学

Archives of Microbiology Pub Date : 2025-09-26 DOI: 10.1007/s00203-025-04464-3

Bikramaditya Behera, Rajrattan Singh, Komal Sharma, Ansh Rai, Shreya Singh, Biji Balan

{"title":"A comprehensive review of advanced strategies to combat antimicrobial resistance","authors":"Bikramaditya Behera, Rajrattan Singh, Komal Sharma, Ansh Rai, Shreya Singh, Biji Balan","doi":"10.1007/s00203-025-04464-3","DOIUrl":"10.1007/s00203-025-04464-3","url":null,"abstract":"<div>Antimicrobial Resistance (AMR) is a growing global issue, as many first-line antibiotics are becoming less effective due to their overuse and misuse. Recent advances in novel antibiotic derivatives reveal mechanisms designed to counteract AMR. Even though conventional antimicrobial therapy has failed, no new antibiotic class has been developed in the past decade. Consequently, various innovative alternative tactics have been discovered to counteract drug-resistant pathogens. The article reviews novel approaches in combating AMR, which include antimicrobial peptides, phage therapy, CRISPR-Cas gene editing, nanomaterial-based antimicrobials, immunomodulatory agents, innovative physicochemical strategies, and combination therapy. Collectively, these approaches utilize cutting edge technologies that mark a shift from the traditional paradigm of antibiotics to integrated next-generation therapeutics. AMR remains a serious issue despite all of the noted advancements, and hence, a collaborative and multidisciplinary action involving researchers, healthcare professionals, policymakers, and pharmaceutical sector is urgently required. The emergence and burden of AMR can be better tackled by inventiveness, cooperation, and proactive approaches.</div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 11","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145147445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Progress and perspectives on microbial dye remediation: enzymatic pathways, microbial diversity, and hybrid microbial-based technologies 微生物染料修复的进展与展望：酶途径、微生物多样性和混合微生物技术。

IF 2.6 3区生物学

Archives of Microbiology Pub Date : 2025-09-26 DOI: 10.1007/s00203-025-04477-y

Sreedeep Dey, Barnali Mandal

{"title":"Progress and perspectives on microbial dye remediation: enzymatic pathways, microbial diversity, and hybrid microbial-based technologies","authors":"Sreedeep Dey, Barnali Mandal","doi":"10.1007/s00203-025-04477-y","DOIUrl":"10.1007/s00203-025-04477-y","url":null,"abstract":"<div>\u0000 \u0000 Synthetic dyes are extensively used in the textile industry and related industries. These structurally stable dyes persist in aquatic systems and cause severe ecotoxicological and human health risks. Widely applied conventional physicochemical treatments are constrained by high costs, sludge production, and incomplete mineralization. This review emphasizes the microbial diversity (i.e., fungi, bacteria, yeast, algae, and consortia) and enzymatic (azoreductase, laccases, peroxidase, etc.) machinery responsible for the reductive and oxidative transformation of recalcitrant dyes. Accessible plate and spectrophotometric assays are some enzymatic identification methods that have been discussed alongside some scalable production strategies (i.e., immobilization, fed-batch, and continuous systems). Upstream resource demand has been highlighted in sustainability assessments, but reduced sludge, less chemical input, and energy recovery revealed downstream gains. Furthermore, compared with conventional adsorbents, hybrid microbial technologies have demonstrated a broader substrate range and technoeconomic competitiveness. While, under optimized laboratory conditions, 80–100% decolorization could be achieved, scaling up to real wastewater matrices, maintaining the stability of microbial consortia, and ensuring the biosafety of nanomaterials are some persistent challenge. Future studies should include long-term pilot trials, omics-assisted microbial design, multispecies microcosm studies, and biomass valorization.\u0000 </div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 11","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145147456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0