Archives of Microbiology最新文献

{"title":"Broad host range phage LPC-1 reduce the risk of Listeria monocytogenes contamination in different food matrices.","authors":"Qian Chong, Ziqiu Fan, Yonghui Ma, Kunzhong Zhang, Jing Deng, Jinrui Ma, Xuehui Zhao, Ji Zhi, Haohao Zhang, Zengwen He, Qing Cao, Huiwen Xue, Huitian Gou","doi":"10.1007/s00203-025-04348-6","DOIUrl":"https://doi.org/10.1007/s00203-025-04348-6","url":null,"abstract":"<p><p>Listeria monocytogenes forms biofilms in food and food-processing environments, entering food through cross-contamination. Phages, as antimicrobial agents, have demonstrated efficacy in addressing this issue. This study demonstrated that the LM (Listeria monocytogenes, LM) lytic phage LPC-1 isolated from livestock slaughterhouse effluent effectively lysed LM, Listeria welshimeri, Listeria innocua, and Enterococcus faecium ATCC 35667. Phage LPC-1 is a tailed phage with a non-contractile long tail, has a short incubation period, high cleavage capacity, and can be adsorbed onto the surface of bacteria within a short period. The LPC-1 phage has a genome spanning 43,466 bp with a GC content of 39% and encompasses 67 coding sequences. Notably, LPC-1's genome displayed significant homologies to various non-Listeria phages. Experimental tests under simulated refrigerated conditions revealed that LPC-1 effectively diminished the presence of LM in milk, pork, and both eggshells and egg liquid, indicating its bacteriostatic properties. Moreover, LPC-1 hindered biofilm formation and enhanced biofilm eradication. Consequently, these findings endorse the potential of phage LPC-1 as a prospective antimicrobial agent specifically suited for controlling LM contamination in the food industry, given its proven safety and efficacy.</p>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 7","pages":"155"},"PeriodicalIF":2.3,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144156032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protein kinase G-a key regulator of pathogenesis in Mycobacterium tuberculosis infection.","authors":"Anjali K Mahato, Rupal Rai, Rashmi Chourasia, Anirudh K Singh, Shivendra K Chaurasiya","doi":"10.1007/s00203-025-04355-7","DOIUrl":"https://doi.org/10.1007/s00203-025-04355-7","url":null,"abstract":"<p><p>Mycobacterium tuberculosis (M. tuberculosis), the causative agent of tuberculosis (TB), remains a leading global health threat, exacerbated by rising drug resistance and the ability of this pathogen to persist within host macrophages. Central to the intracellular survival of M. tuberculosis is Protein Kinase G (PknG), a secreted, eukaryotic-like serine/threonine kinase that subverts host immune defenses and modulates bacterial physiology. This review provides a comprehensive overview of structural features and the multifaceted role of PknG in M. tuberculosis pathogenesis, including inhibition of phagosome-lysosome fusion, acid tolerance, metabolic reprogramming, autophagy suppression, and cell wall remodelling. Additionally, we discuss recent advancements in targeting PknG with small-molecule inhibitors, highlighting its promise as a therapeutic target. By delineating PknG's central role in host-pathogen interactions and stress adaptation, this review underscores its potential in shaping future anti-TB strategies, especially against drug-tolerant and latent infections..</p>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 7","pages":"154"},"PeriodicalIF":2.3,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144156034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship between antibiotic resistance and biofilm-forming capacity with RND efflux pumps expression in clinical Acinetobacter baumannii isolates","authors":"Zahra Farshadzadeh,&nbsp;Saeed Khoshnood,&nbsp;Sousan Akrami,&nbsp;Effat Abbasi Montazeri,&nbsp;Melika Moradi,&nbsp;Sara Masihzadeh,&nbsp;Sara Daneshfar,&nbsp;Tahereh Navidifar","doi":"10.1007/s00203-025-04347-7","DOIUrl":"10.1007/s00203-025-04347-7","url":null,"abstract":"<div><p>This study investigated the gene expression pattern of resistance-nodulation-division (RND) efflux pumps, in <i>Acinetobacter baumannii</i> isolates with a focus on their association with carbapenem resistance and biofilm formation ability. A collection of 102 <i>A. baumannii</i> isolates was evaluated for antibiotic susceptibility testing using an automated broth microdilution technique. The ability of these isolates to form biofilms was evaluated using a standardized protocol. The isolates were genotyped using the Multiple-Locus Variable number tandem repeat Analysis-8 (MLVA-8) method and the gene expression levels of the RND efflux pump genes were quantified by real-time PCR. Results showed widespread antibiotic resistance, with 85% of isolates classified as multidrug resistant. Genotyping results identified 32 different MLVA types organized into six clusters (A–F) and 17 unique genotypes. The majority of isolates demonstrated the ability to form biofilms, and an inverse relationship was observed between biofilm formation and carbapenem resistance. The expression of the <i>adeB</i> gene was significantly increased in carbapenem-non-susceptible <i>A. baumannii</i> isolates. In addition, the expression of the <i>adeG</i> gene was 2.08 times higher in isolates capable of forming moderate to strong biofilms compared to those forming weak biofilms. The novelty of this study is a new insight into the relationship between efflux pump expression, antibiotic resistance and biofilm formation in <i>A. baumannii</i>, as well as AdeABC overexpression in carbapenem-resistant isolates and AdeFGH overexpression in biofilm-forming strains, providing potential therapeutic targets. These findings suggest that targeting RND efflux pumps may be a promising strategy to control survival and antibiotic resistance of <i>A. baumannii</i> isolates through biofilm inhibition.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 7","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144131481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Viral tropism in plants, reproductive tissues, and seeds","authors":"Leandro Alberto Núñez-Muñoz,&nbsp;Berenice Calderón-Pérez,&nbsp;Roberto Ruiz-Medrano,&nbsp;Beatriz Xoconostle-Cázares,&nbsp;Rodolfo de la Torre-Almaraz","doi":"10.1007/s00203-025-04353-9","DOIUrl":"10.1007/s00203-025-04353-9","url":null,"abstract":"<div><p>Plant viral tropism refers to virus ability for infecting and replicating within specific cell types, tissues or hosts. Plant viral tropism is shaped by the absence of specific membrane-associated viral receptors and the supracellular nature of viral transport through plasmodesmata and vascular tissues. This review focuses on the molecular and cellular determinants of plant viral tropism, including modifications in plasmodesmal permeability, host-mediated RNA silencing, and tissue-specific viral protein localization. We discuss how certain viruses target reproductive organs, meristems, and seeds, overcoming antiviral barriers to establish persistent infections. Additionally, we explore the role of host factors in shaping viral distribution. Advances in super-resolution microscopy, single-cell transcriptomics, and proteomics have significantly expanded our ability to dissect virus-host interactions at the nanoscale, uncovering new mechanisms of viral accumulation. Understanding these processes is essential not only for improving crop resistance and designing integrated disease management strategies, but also for repurposing plant viruses as tools for targeted delivery and biotechnological applications.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 7","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00203-025-04353-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144117669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unravelling fungal genome editing revolution: pathological and biotechnological application aspects","authors":"Abdallah M. A. Hassane,&nbsp;Marwa Obiedallah,&nbsp;Javad Karimi,&nbsp;Sadat M. R. Khattab,&nbsp;Hussein R. Hussein,&nbsp;Youssef Abo-Dahab,&nbsp;Adel Eltoukhy,&nbsp;Nageh F. Abo-Dahab,&nbsp;Mohamed E. Abouelela","doi":"10.1007/s00203-025-04360-w","DOIUrl":"10.1007/s00203-025-04360-w","url":null,"abstract":"<div><p>Fungi represent a broad and evolutionarily unique group within the eukaryotic domain, characterized by extensive ecological adaptability and metabolic versatility. Their inherent biological intricacy is evident in the diverse and dynamic relationships they establish with various hosts and environmental niches. Notably, fungi are integral to disease processes and a wide array of biotechnological innovations, highlighting their significance in medical, agricultural, and industrial domains. Recent advances in genetic engineering have revolutionized fungal research, with CRISPR/Cas emerging as the most potent and versatile genome editing platform. This technology enables precise manipulation of fungal genomes, from silencing efflux pump genes in <i>Candida albicans</i> (enhancing antifungal susceptibility) to targeting virulence-associated sirtuins in <i>Aspergillus fumigatus</i> (attenuating pathogenicity). Its applications span gene overexpression, multiplexed mutagenesis, and secondary metabolite induction, proving transformative for disease management and biotechnological innovation. CRISPR/Cas9’s advantages—unmatched precision, cost-effectiveness, and therapeutic potential—are tempered by challenges like off-target effects, ethical dilemmas, and regulatory gaps. Integrating nanoparticle delivery systems and multi-omics approaches may overcome technical barriers, but responsible innovation requires addressing these limitations. CRISPR-driven fungal genome editing promises to redefine solutions for drug-resistant infections, sustainable bioproduction, and beyond as the field evolves. In conclusion, genome editing technologies have enhanced our capacity to dissect fungal biology and expanded fungi’s practical applications across various scientific and industrial domains. Continued innovation in this field promises to unlock the vast potential of fungal systems further, enabling more profound understanding and transformative biotechnological progress.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 7","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144108660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"One-step narrow-thermal-cycling strand exchange amplification for sensitive detection of porcine reproductive and respiratory syndrome virus","authors":"Linlin Zhuang,&nbsp;Chunlei Song,&nbsp;Li Sun,&nbsp;Haiqiang Xie,&nbsp;Liqun Wang,&nbsp;Qingxin Liu,&nbsp;Hongjing Shi,&nbsp;Jianbo Yang,&nbsp;Qiuping Shen","doi":"10.1007/s00203-025-04351-x","DOIUrl":"10.1007/s00203-025-04351-x","url":null,"abstract":"<div><p>Porcine reproductive and respiratory syndrome (PRRS), which is primarily characterized by respiratory and reproductive dysfunction, is an epidemic disease caused by porcine reproductive and respiratory syndrome virus (PRRSV) that has the potential to economically devastate the global swine industry. Rapid and accurate detection of PRRSV is critical for effective control of PRRS in swine. In this study, a novel one-step, highly sensitive and specific accelerated strand exchange amplification (ASEA) method for the detection of PRRSV was developed. The detection limit of the ASEA method was determined to be 6 copies µL^–1 of PRRSV RNA reference material or PRRSV in spiked swine blood. The ASEA method demonstrated the capacity to discern the currently circulating PRRSV genotypes without cross-reactivity with other porcine-derived pathogens that manifest similar clinical signs. The ASEA method exhibited a detection time of 35 min, and its clinical applicability was validated through the analysis of 5 inactivated blood samples and 62 clinical samples. The method’s potential extends beyond the diagnosis of PRRSV, as it can also be applied to the rapid diagnosis of other RNA pathogens. This capacity is expected to make significant contributions to future epidemic prevention and surveillance efforts.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 7","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144108445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glucose starvation induces tau phosphorylation leading to cellular stress response in fission yeast","authors":"Merve Yılmazer,&nbsp;Aslıhan Şengelen,&nbsp;Yunus Aksüt,&nbsp;Bedia Palabıyık,&nbsp;Evren Önay-Uçar,&nbsp;Semian Karaer Uzuner","doi":"10.1007/s00203-025-04350-y","DOIUrl":"10.1007/s00203-025-04350-y","url":null,"abstract":"<div><p>Misfolded tau proteins and their accumulation cause many neurodegenerative diseases named tauopathies. While phosphorylation is required for tau protein activity, hyperphosphorylation leads to pathological conditions. Previous reports have shown that glucose deprivation might influence tau protein formation and phosphorylation in vivo, though its effect on cellular stress pathways in a yeast model has not been documented. In this study, we examined the various cellular processes, including oxidative and ER stress responses, glucose metabolism, autophagy, 20 S proteasomal activity, and glucose consumption in <i>Schizosaccharomyces pombe</i> cells heterologously expressing the human <i>MAPT</i> gene, which we obtained in our previous study. We observed increased levels of <i>MAPT</i> gene expression, phosphorylated tau protein (sites at Thr181, Thr231, and Ser396), and phosphorylated GSK-3β (site at Tyr216; contributes to tau phosphorylation) under glucose starvation conditions. The presence of tau protein led to increased expression levels of genes related to oxidative stress response and ER stress in fission yeast. Glucose-starved yeast expressing tau showed higher proteasomal activity and autophagy than control cells in normal glucose conditions. Additionally, cells containing tau protein exhibited higher glucose consumption under nutrient starvation conditions than those lacking tau. These findings indicate a possible relationship between increased tau protein phosphorylation and glucose metabolism, supporting the connection among tauopathies, poorly regulated blood sugar, and diabetes; thus, this provides initial evidence that <i>S. pombe</i> yeast can serve as a model for research in this area.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 7","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144084868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Current status and future prospects of microalgae-based degradation of spent lubricant oil hydrocarbon towards environmental sustainability: a mini review and bibliometric analysis","authors":"Stella B. Eregie,&nbsp;Isaac A. Sanusi,&nbsp;Olaniran O. Ademola","doi":"10.1007/s00203-025-04332-0","DOIUrl":"10.1007/s00203-025-04332-0","url":null,"abstract":"<div><p>The biodegradation of spent oil waste (SOW) using bacteria and fungi has been actively researched over the years. Only recently has the use of microalgae for the treatment of SOW attracted significant attention. This review aims to highlight the biodegradative capabilities of microalgae as well as provide a comprehensive bibliometric analysis to assess current research activities and trends in microalgae-based biodegradation of SOW. The bibliographic data exported from Dimensions database was analyzed using VOSviewer, focusing on various aspects such as document types, publications, subject categories, sources, countries, authors, organizations, and cited articles. The results obtained showed a remarkable increase (80.23%) in the number of article publications from 2005 to 2023 in this field of research. China (887 publications), Environmental Science (3571 publications), Bioresource Technology (249 publications) and Harbin Institute of Technology (72 publications), were the most productive country, subject category, journal, and organization, respectively, publishing articles in this field of research. The review also discussed SOW hydrocarbons ranging from alkanes, aromatic compounds to polychlorinated compounds and the mechanism of degradation of these compounds by microalgae. Overall, the review provided useful insight on microalgae SOW degradation, current research direction and the prospect of using microalgae in environmental remediation and sustainability.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 7","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00203-025-04332-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144090947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Generation and validation of a versatile inducible multiplex CRISPRi system to examine bacterial regulation in the Euprymna-Vibrio fischeri symbiosis","authors":"Brian Lynn Pipes,&nbsp;Michele Kiyoko Nishiguchi","doi":"10.1007/s00203-025-04354-8","DOIUrl":"10.1007/s00203-025-04354-8","url":null,"abstract":"<div><p>The <i>Vibrio fischeri</i>—<i>Euprymna scolopes</i> symbiosis has become a powerful animal—microbe model system to examine the genetic underpinnings of symbiont development and regulation. Although there has been a number of elegant bacterial genetic technologies developed to examine this symbiosis, there is still a need to develop more sophisticated methodologies to better understand complex regulatory pathways that lie within the association. Therefore, we have developed a suite of CRISPR interference (CRISPRi) vectors for inducible repression of specific <i>V. fischeri</i> genes associated with symbiotic competence. The suite utilizes both Tn7-integrating and shuttle vector plasmids that allow for inducible expression of CRISPRi dCas9 protein along with single-guide RNAs (sgRNA) modules. We validated this CRISPRi tool suite by targeting both exogenous (an introduced mRFP reporter) and endogenous genes (<i>luxC</i> in the bioluminescence producing <i>lux</i> operon, and f<i>lrA</i>, the major regulatory gene controlling flagella production). The suite includes shuttle vectors expressing both single and multiple sgRNAs complementary to the non-template strand of multiple targeted genetic loci, which were effective in inducible gene repression, with significant reductions in targeted gene expression levels. <i>V. fischeri</i> cells harboring a version of this system targeting the <i>luxC</i> gene and suppressing the production of luminescence were used to experimentally validate the hypothesis that continuous luminescence must be produced by the symbiont in order to maintain the symbiosis at time points longer than the known 24-h limit. This robust new CRISPRi genetic toolset has broad utility and will enhance the study of <i>V. fischeri</i> genes, bypassing the need for gene disruptions by standard techniques of allelic knockout-complementation-exchange and the ability to visualize symbiotic regulation in vivo.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 7","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00203-025-04354-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144074109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RsmG methylation of 16S rRNA affects the function of ribosomal protein uS12","authors":"Trevor W. Bell,&nbsp;Rowan M. Turner,&nbsp;Amanda M. Merryman,&nbsp;Juliana J. Joseph,&nbsp;Steven T. Gregory,&nbsp;Michael O’Connor","doi":"10.1007/s00203-025-04349-5","DOIUrl":"10.1007/s00203-025-04349-5","url":null,"abstract":"<div><p>The RsmG methyltransferase modifies G527 in bacterial 16S rRNA and its inactivation confers low level streptomycin resistance. In contrast, high level streptomycin resistance typically requires specific alterations in ribosomal protein uS12 or 16S rRNA. Here, we have asked if <i>rsmG</i> inactivation alters the phenotypes of any of a collection of randomly-generated <i>Escherichia coli</i> uS12 mutants. While several uS12 mutants show moderately increased resistance to streptomycin when <i>rsmG</i> is inactivated (MIC = 10–40 µg/ml), a uS12 R85H/<i>rsmG</i>-inactivated strain uniquely displays very high resistance (MIC &gt; 1,024 µg/ml). Additional genetic selections showed that <i>rsmG</i> null mutations combined with specific alterations in uS12 can generate streptomycin-dependence, or pseudo-dependence, in addition to resistance. Moreover, growth of several of these mutants on high concentrations of streptomycin is conditional on <i>rsmG</i> inactivation. Thus, loss of m⁷G527 methylation affects the streptomycin phenotypes of distinct uS12 mutants and identifies an additional route to high-level streptomycin resistance in bacteria.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 7","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144073616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}