Archives of Microbiology最新文献

Design and development of a novel multi-epitope DNA vaccine candidate against infectious bronchitis virus: an immunoinformatic approach

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2025-03-11 DOI: 10.1007/s00203-025-04283-6

Haoyu Liu, Tingting Liu, Xinyuan Wang, Xiaochen Zhu, Jinling He, Hui Wang, Aili Fan, Dongchao Zhang

{"title":"Design and development of a novel multi-epitope DNA vaccine candidate against infectious bronchitis virus: an immunoinformatic approach","authors":"Haoyu Liu, Tingting Liu, Xinyuan Wang, Xiaochen Zhu, Jinling He, Hui Wang, Aili Fan, Dongchao Zhang","doi":"10.1007/s00203-025-04283-6","DOIUrl":"10.1007/s00203-025-04283-6","url":null,"abstract":"<div><p>Avian infectious bronchitis (IB) is one of the major respiratory diseases in poultry. At present, attenuated vaccines are the main commercial vaccines, but they have many defects. We aimed to construct a novel multi-epitope DNA vaccine based on avian infectious bronchitis virus (IBV) S1 and N proteins for the prevention of IBV infection. We screened the dominant B and T cell epitopes of target proteins utilizing epitope prediction tools. A new high-immunogenicity epitope peptide segment named QSN was designed and screened by linking peptide. The physicochemical properties of QSN were analyzed by bioinformatics. The recombinant plasmid pEGFP-QSN was obtained by inserting the synthesized QSN gene into the eukaryotic expression vector pEGFP-N1. On the 7th day of age, chicks were immunized by intramuscular injection of the plasmid, and serum specific antibody IgG, cytokines IFN-γ and IL-2, and T lymphocyte subsets were detected after booster immunization. Bioinformatics analysis showed that QSN had high hydrophilicity without transmembrane region and stable structure after binding to receptor. The recombinant eukaryotic vector was successfully constructed. Two weeks after booster immunization, compared with NS group and pEGFP-N1 group, serum IgG level, concentrations of cytokines IFN-γ and IL-2, and proportion of CD4<sup>+</sup> T lymphocytes in pEGFP-QSN group were significantly increased (<i>P</i> < 0.01 or <i>P</i> < 0.05). Collectively, the multi-epitope DNA could stimulate humoral and cellular immune responses in chickens and is expected to be a potential vaccine candidate against IBV infection.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143594694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of intestinal bacteria for the production of quercetin and isoquercitrin from rutin

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2025-03-10 DOI: 10.1007/s00203-025-04278-3

Narantsetseg Byambaakhuu, Shen Duan, Ren Sa, Qing-lan Yang, Hai-Yan Xu, Cheng-Bin Shan, Ri-hua Xu, Chao-Mei Ma

{"title":"Characterization of intestinal bacteria for the production of quercetin and isoquercitrin from rutin","authors":"Narantsetseg Byambaakhuu, Shen Duan, Ren Sa, Qing-lan Yang, Hai-Yan Xu, Cheng-Bin Shan, Ri-hua Xu, Chao-Mei Ma","doi":"10.1007/s00203-025-04278-3","DOIUrl":"10.1007/s00203-025-04278-3","url":null,"abstract":"<div><p>This study was to evaluate the potential of human intestinal bacterial species in the biotransformation of rutin to quercetin and isoquercitrin which is rarer than rutin in nature and could more potently inhibit the growth of some cancer cell lines. Bacterial strains isolated from healthy human fecal samples were identified through 16S rDNA gene sequence analysis and genome analysis. Isoquercitrin and quercetin were identified and quantified by UHPLC-QQQ-MS in multiple reaction monitoring mode. As results, the intestinal bacterial strains, comprising nine Gram-positive rods and one Gram-negative rod, were classified into <i>Enterococcus</i>, <i>Lactococcus</i>, and <i>Escherichia</i> genera. Among the ten isolates<i>, Lactococcus garvieae</i> Y3-2 and <i>Lactococcus petauri</i> Y5-4 produced higher amounts of quercetin compared to other bacteria. Interestingly, all strains of <i>Enterococcus faecium</i> species (Y4-1, Y4-2, Y5-1, and Y5-2) exhibited a relatively strong ability to convert rutin to isoquercitrin, with Y4-2 being particularly efficient. The higher L-rhamnosidase activity observed in <i>E. faecium</i> Y4-1 and <i>E. faecium</i> Y4-2 correlated with their significant yield of isoquercitrin. Four or three genes were probably involved in rutin metabolism according to the analysis of flavonoid pathway based on genome sequences. The results provided information for selecting bacterial species to convert rutin into target bioactive compounds, and for purification of pure enzymes to biosynthesize isoquercitrin.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143583406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Yeast’s vacuole a privileged niche that protects intracellular bacteria against antibiotics

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2025-03-10 DOI: 10.1007/s00203-025-04281-8

Atousa Hatefi, Farideh Siavoshi, Saman Khalili-Samani

{"title":"Yeast’s vacuole a privileged niche that protects intracellular bacteria against antibiotics","authors":"Atousa Hatefi, Farideh Siavoshi, Saman Khalili-Samani","doi":"10.1007/s00203-025-04281-8","DOIUrl":"10.1007/s00203-025-04281-8","url":null,"abstract":"<div><p>Detection of <i>Helicobacter pylori</i>, <i>Staphylococcus</i>, <i>Nocardia</i> and <i>Cyanobacteria</i> inside the yeast <i>Candida tropicalis</i> raised the question whether treating yeast with antibiotics mix (ABM) eliminates intracellular bacteria. Live/Dead staining showed occurrence of viable bacteria inside the vacuole of <i>C. tropicalis</i>. Amplification of bacterial <i>16S rRNA</i> genes from yeast DNA with the size of 521, 750, 606 and 450 bp were similar to those from control <i>H. pylori</i>, <i>Staphylococcus</i>, <i>Nocardia</i> and <i>Cyanobacteria</i>, respectively. To eliminate intracellular bacteria yeast cultures in yeast-glucose (YG) broth were treated with 32–1024 μg/mL of ABM (amoxicillin, ciprofloxacin, rifampin and metronidazole) for up to 24 h. Viability of treated yeast cells and their intracellular bacteria was assessed by colony count, Live/Dead staining and detection of bacterial <i>16S rRNA</i> genes. Colony count of <i>C. tropicalis</i> exposed to 32–256 μg/mL of ABM (4.39–9.63) or 512–1024 μg/mL (9.67–9.77) were similar to their respected controls (p > 0.05). Amplification of similar bacterial genes from treated yeasts and controls confirmed persistent occurrence of intracellular bacteria. Micrographs of yeasts treated with 32–256 μg/mL of ABM showed intact yeasts and intracellular bacteria, however those treated with 512 and 1024 μg/mL showed occurrence of < 10% and > 10% yellow damaged yeasts, respectively that accumulated yellow rifampin. Fluorescence microscopy showed that both intact and damaged yeasts carried live bacteria inside their vacuole. Culture of treated yeasts on YG agar produced colonies with totally intact yeasts and intracellular bacteria. Yeast extruded antibiotics and reduced their effective concentration for killing intracellular bacteria. Establishment of bacteria inside the fungal vacuole cannot be disrupted with antibiotics.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00203-025-04281-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143581186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developing a mutant strain of Pseudomonas composti ODT-54 for enhanced production of Psl extracellular polysaccharide

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2025-03-07 DOI: 10.1007/s00203-025-04292-5

Jieer Wu, Feng Han, Yongxin Yu, Yongjie Wang

引用次数: 0

Antibacterial and anti-biofilm activities of Derazantinib (ARQ-087) against Staphylococcus aureus

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2025-03-06 DOI: 10.1007/s00203-025-04288-1

Weiguo Wang, Qiuxiang Zhong, Xincheng Huang

{"title":"Antibacterial and anti-biofilm activities of Derazantinib (ARQ-087) against Staphylococcus aureus","authors":"Weiguo Wang, Qiuxiang Zhong, Xincheng Huang","doi":"10.1007/s00203-025-04288-1","DOIUrl":"10.1007/s00203-025-04288-1","url":null,"abstract":"<div><p>The global rise of multidrug-resistant pathogens, particularly methicillin-resistant <i>Staphylococcus aureus</i> (MRSA), represents a critical public health challenge. This study evaluates the antibacterial and anti-biofilm activities of Derazantinib (ARQ-087) against <i>S. aureus</i>. ARQ-087 exhibited minimum inhibitory concentration (MIC) values ranging from 4 to 16 µM against <i>S. aureus</i> reference laboratory strains and diverse clinical MRSA isolates, demonstrating strong antibacterial activity with minimal resistance development. Time-kill assays demonstrated a concentration- and time-dependent reduction in bacterial viability. Crystal violet staining assays revealed that ARQ-087 significantly inhibited MRSA biofilm formation in a dose-dependent manner. Additionally, ARQ-087 exhibited strong anti-biofilm activity against pre-formed biofilms, as shown by colony counts and confocal laser scanning microscopy, which indicated extensive biofilm disruption and bacterial cell death. Mechanistic studies revealed that ARQ-087 disrupts bacterial membrane integrity, as evidenced by SYTOX Green and DISC3(5) fluorescence assays, while inducing intracellular ATP depletion and reactive oxygen species generation, contributing to bacterial death. ARQ-087 also displayed negligible hemolytic activity and no acute toxicity observed in a <i>Galleria mellonella</i> infection model. In this model, ARQ-087 prolonged the survival of larvae infected with <i>S. aureus</i>. These findings highlight ARQ-087 as a promising therapeutic candidate for treating MRSA infections and biofilm-associated diseases. Further preclinical studies are needed to confirm its potential for clinical application.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143554151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Although invisible, fungi are recognized as the engines of a microbial powerhouse that drives soil ecosystem services

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2025-03-06 DOI: 10.1007/s00203-025-04285-4

Shova Akter, Upoma Mahmud, Baig Abdullah Al Shoumik, Md. Zulfikar Khan

{"title":"Although invisible, fungi are recognized as the engines of a microbial powerhouse that drives soil ecosystem services","authors":"Shova Akter, Upoma Mahmud, Baig Abdullah Al Shoumik, Md. Zulfikar Khan","doi":"10.1007/s00203-025-04285-4","DOIUrl":"10.1007/s00203-025-04285-4","url":null,"abstract":"<div><p>Soil ecosystem services (SES) are the benefits that humans derive from soil. These services emerge from the complex interactions between biotic and abiotic processes within soil systems. They are vital for maintaining ecosystem resilience and ensuring long-term sustainability. Soil hosts a diverse group of biota, among them fungi play a crucial role in supporting and enhancing SES due to their remarkable adaptability and ability to thrive under unfavorable conditions. This review explores the multifaceted roles of fungi in SES, emphasizing their growing importance in strengthening ecosystem resilience and climate change adaptation. Fungi significantly contribute to the key ecosystem processes such as soil aggregation, organic matter (OM) decomposition, nutrients cycling, plant productivity, and carbon (C) sequestration. However, potential threats to fungal abundance and diversity could undermine these critical functions, highlighting the need for proactive measures to preserve fungal communities. The pivotal role of fungi in SES, including agricultural production and climate regulation, tailor them as indispensable microbial engines that shape and maintain ecosystem resilience. Emerging evidence suggests that soil fungal communities may become increasingly prominent under the future climate scenarios. Thus, understanding how fungal functional roles evolve in response to climate change is emergent for safeguarding SES and ensuring environmental sustainability. Furthermore, the co-occurrance of fungi with other soil organisms in supporting SES highlights the need to integrate diverse soil biota alongside fungi to promote sustainable SES. Collaborative efforts to comprehend and manage soil microbial communities are imperative for maintaining the long-term ecological stability of ecosystems.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143554150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beyond the pink: uncovering the secrets of pink pigmented facultative methylotrophs

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2025-03-06 DOI: 10.1007/s00203-025-04280-9

Nayana Grace Koshy, S. Anu Rajan, K. N. Anith, N. Chitra, V. I. Soumya, Thanku Mariam Scaria, R. Beena

{"title":"Beyond the pink: uncovering the secrets of pink pigmented facultative methylotrophs","authors":"Nayana Grace Koshy, S. Anu Rajan, K. N. Anith, N. Chitra, V. I. Soumya, Thanku Mariam Scaria, R. Beena","doi":"10.1007/s00203-025-04280-9","DOIUrl":"10.1007/s00203-025-04280-9","url":null,"abstract":"<div><p>Pink Pigmented Facultative Methylotrophs (PPFMs) belong to a diverse group of methylotrophic bacteria predominantly in the genus <i>Methylobacterium</i>, and are known for their beneficial interactions with plants. They can use single-carbon compounds, such as methanol, formate, formaldehyde and methyl amines as well as various multi-carbon substrates as sources of carbon and energy. PPFMs are characterized by their distinctive pink pigmentation and are commonly found in the phyllosphere, where they play a major role in promoting plant growth through various mechanisms; These mechanisms include the production of phytohormones, enhancing nutrient acquisition, mitigating abiotic stresses and providing biocontrol of phytopathogens. Due to their eco-friendly nature PPFMs are viewed as promising alternatives to synthetic fertilizers and pesticides in green agriculture. Furthermore, the ecological significance of PPFMs extends beyond their direct interactions with host plants. They also contribute to the resilience of ecosystems by participating in the cycling of nutrients in the environment. As the importance of the plant microbiome in agriculture becomes more recognized, the potential of PPFMs to support sustainable farming practices and contribute to environmental health is increasingly evident. This underscores their relevance in addressing global agricultural challenges.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143554101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of melanin from the fungus Scolecobasidium Musae and its antioxidant and photoprotective properties

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2025-03-06 DOI: 10.1007/s00203-025-04279-2

Fatma Meryem Bozhuyuk, Murat Ozdal

{"title":"Characterization of melanin from the fungus Scolecobasidium Musae and its antioxidant and photoprotective properties","authors":"Fatma Meryem Bozhuyuk, Murat Ozdal","doi":"10.1007/s00203-025-04279-2","DOIUrl":"10.1007/s00203-025-04279-2","url":null,"abstract":"<div><p>Melanin is a dark colored pigment produced by various living organisms. In this study, the melanin-producing fungus was isolated from a washing machine and identified as <i>Scolecobasidium musae</i> by 18 S rDNA analysis. Melanin production by <i>S. musae</i> was reported for the first time. Melanin was obtained from the dark-colored biomass produced by <i>S. musae</i> in submerged culture. The pigment was then purified, characterized, and identified. It was determined that purified melanin was dissolved easily in alkaline solutions but insoluble in organic solvents. Devices such as UV-Vis spectroscopy, XRD, FTIR, NMR, EPR, and elemental analysis were used to determine the chemical content and structure of the produced melanin. The melanin synthesis pathway was investigated using melanin inhibitors and by performing LC-MS analysis of the fermentation medium in which <i>S. musae</i> grew. It was found that the newly produced melanin from <i>S. musae</i> possesses antioxidant and photoprotective properties (sun protection and UV protection for <i>Staphylococcus aureus</i>). These results provide new possibilities for the biotechnological applications of this naturally producing melanin pigment.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143554152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Computational insights into the targeted inhibition of lipoxygenase in Pseudomonas aeruginosa: hints for drug design

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2025-03-04 DOI: 10.1007/s00203-025-04257-8

Sahanawaz Parvez, Sonam Grewal, Anamika Kumari, Polamarasetty Aparoy

{"title":"Computational insights into the targeted inhibition of lipoxygenase in Pseudomonas aeruginosa: hints for drug design","authors":"Sahanawaz Parvez, Sonam Grewal, Anamika Kumari, Polamarasetty Aparoy","doi":"10.1007/s00203-025-04257-8","DOIUrl":"10.1007/s00203-025-04257-8","url":null,"abstract":"<div><p><i>Pseudomonas aeruginosa</i> is regarded as the most opportunistic pathogen. It can induce ferroptosis in humans. It secretes a unique lipoxygenase (LOX) isoform, pLoxA that can oxidize polyenoic fatty acids. Unlike other lipoxygenases, pLoxA can oxygenate membrane phospholipids like phosphatidylethanolamine, leading to hemolysis of red blood cells (RBC). This functional overlap with human 15-LOX that uses the same substrate has provided a bottleneck to the discovery of pLoxA-specific inhibitors and there is an immediate need to find pLoxA specific drugs. The active site of pLoxA is much larger than LOX enzymes, reflecting its ability to accommodate bulky substrates, such as phospholipids. The molecular docking of two experimentally established inhibitors and the further molecular dynamics simulations provided possible key residues in the active site of pLoxA. Our study found that this region is essentially hydrophobic including His 377 and His 382 that are placed to the non-heme iron atom and help to stabilize the inhibitors in the binding site along with hydrophobic residues contribute well toward ligand interactions that involve Phe 415, Ile 416 and Leu 424. MD simulations showed that interactions with those residues were dynamic in nature. Main contribution to binding stability arose via π-π stacking, π-cation, and alkyl interactions.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143533102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Akkermansia muciniphila: promises and pitfallsfor next-generation beneficial microorganisms

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2025-03-04 DOI: 10.1007/s00203-025-04263-w

Yantong Liu, Zonglun Li, Sze Ching Lee, Shurui Chen, Feifei Li

{"title":"Akkermansia muciniphila: promises and pitfallsfor next-generation beneficial microorganisms","authors":"Yantong Liu, Zonglun Li, Sze Ching Lee, Shurui Chen, Feifei Li","doi":"10.1007/s00203-025-04263-w","DOIUrl":"10.1007/s00203-025-04263-w","url":null,"abstract":"<div><p><i>Akkermansia muciniphila</i>, a microorganism ubiquitously colonizing the mucosal layer of the human gut, has garnered significant scientific interest as a promising candidate for probiotic therapeutics. Its persistent identification in both laboratory and living organism studies underscores its potential physiological benefits, positioning it as a bacterium of paramount importance in promoting host health. This review examines the diversity and abundance of gut microbiota members, emphasizing the identification of microbial species engaged in cross-feeding networks with <i>A. muciniphila</i>. Insightful exploration into the mechanisms of cross-feeding, including mucin-derived nutrient exchange and metabolite production, unveils the intricate dynamics shaping microbial community stability. Such interactions contribute not only to the availability of essential nutrients within the gut environment but also to the production of metabolites influencing microbial community dynamics and host health. In conclusion, the cumulative evidence from in vitro and in vivo perspectives substantiates the notion that <i>A. muciniphila</i> holds tremendous promise as a next-generation probiotic. By leveraging its unique physiological benefits, particularly in mucosal health and metabolic regulation, <i>A. muciniphila</i> stands poised to revolutionize the landscape of probiotic interventions for enhanced host well-being.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143533101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0