Archives of Microbiology最新文献

{"title":"Development and application of an interbacterial DNA delivery system based on M13 bacteriophage","authors":"Li Qu,&nbsp;Zhou Chi,&nbsp;Bang-Ce Ye","doi":"10.1007/s00203-025-04309-z","DOIUrl":"10.1007/s00203-025-04309-z","url":null,"abstract":"<div><p>With the rapid development in synthetic biology, bacterial therapy has been widely applied in disease diagnosis and treatment. A key step in regulating the execution of specific instructions by engineered bacteria in vivo involves the transfer of information between bacteria. Currently, small molecule compounds and proteins are commonly used as carriers for inter-bacterial information transfer. However, DNA, as an information carrier, boasts higher information capacity and ease of editing. Based on this, we have developed an engineered bacterial DNA delivery system using M13 bacteriophage to study the cooperative response mechanisms between bacteria. Firstly, we leveraged the ability of engineered bacteria to secrete phagemid DNA into the extracellular environment via bacteriophages and to acquire phagemid DNA through infection by extracellular phagemids, thereby developing a dynamic phagemid replenishment system. This system can maintain the stability of phagemid DNA in environments lacking antibiotic selection pressure, constructing a stable DNA delivery platform for phagemid output. Subsequently, using this engineered bacterial DNA delivery system, we modularized gene circuits and placed them in two different engineered bacteria. Through DNA transfer, we achieved a cooperative response to signals between the two bacteria, laying the groundwork for multi-bacterial joint regulation. Our research not only provides an effective tool for information transfer in engineered bacteria but also offers a novel approach for multi-bacterial therapy.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 5","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering antibiotic resistance, quorum sensing, and biofilm forming genes of Micrococcus luteus from hemodialysis tunneled cuffed catheter tips of renal failure patients","authors":"Rajsekhar Adhikary,&nbsp;Indrani Sarkar,&nbsp;Dhara Patel,&nbsp;Sishir Gang,&nbsp;Uttam Kumar Nath,&nbsp;Saugata Hazra","doi":"10.1007/s00203-025-04310-6","DOIUrl":"10.1007/s00203-025-04310-6","url":null,"abstract":"<div><p>Catheter-related bloodstream infections create a significant challenge in healthcare system, often complicated by antibiotic resistance and biofilm formation of multi-drug resistance and virulent bacterial pathogens. This study focused on biofilm-forming efficiency, and underlying genetic mechanisms in <i>Micrococcus luteus</i> HL_Chru_C₃, isolated from a hemodialysis catheter tip. The isolate exhibited resistance to multiple antibiotic classes, including beta-lactams and glycopeptides. Biofilm assays revealed that <i>M. luteus</i> HL_Chru_C₃ formed optimum biofilms at high concentration of carbohydrates (500 mM), and pH 5 but there was no significant role of mineral salts. Whole-genome sequencing and bioinformatic analysis using CARD, KAAS, and KEGG databases identified genes associated with antibiotic resistance (<i>ftsI</i>, <i>pbp1a/2</i>, <i>vanY</i>, <i>alr</i>, <i>ddl</i>, <i>murF</i>, <i>mraY</i>, <i>and murG</i>), quorum sensing (genes from the <i>opp</i> family, <i>sec</i>, <i>cylA</i>, <i>ccfA</i>, <i>phnA</i>, <i>phnB</i>, <i>phzC</i>, <i>rpfB</i>, <i>clp</i>, <i>and toxE</i>), and biofilm formation (<i>phnA</i>, <i>phnB</i>, <i>cyaB</i>, <i>vfr</i>, <i>vps</i>, <i>glgC</i>, <i>wecB</i>, <i>wecC</i>, <i>and cysE</i>). The predicted mechanisms of action for these genes, based on homology to other organisms, suggest complex interactions contributing to the observed phenotypes. This study provides an insight into the genetic basis of antibiotic resistance and biofilm formation in <i>M. luteus</i> HL_Chru_C₃ isolated from a hemodialysis catheter, highlighting the need for effective infection control strategies to combat CRBSIs.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 5","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salinicoccus bachuensis sp. nov., a novel halophilic bacterium, isolated from rhizosphere soil surrounding Tamarix chinensis Lour. plants in Bachu County, Xinjiang, China","authors":"Zhen-Pu Liang,&nbsp;Rui Wang,&nbsp;Han-Wen Cao,&nbsp;Xin-Lu Zhou,&nbsp;Jin-Ping Dai,&nbsp;Ping Su,&nbsp;Deepali Singh,&nbsp;Xiao-Xia Zhang","doi":"10.1007/s00203-025-04315-1","DOIUrl":"10.1007/s00203-025-04315-1","url":null,"abstract":"<div><p>The strain Bachu38^T was isolated from the rhizosphere soil of <i>Tamarix chinensis</i> Lour. plants in Bachu County, Xinjiang, China. This strain exhibited growth under a wide range of conditions, with temperature tolerance from 8 to 45°C (optimal at 28°C), pH adaptability between 7.0 and 10.0 (optimal range 8.0–9.0), and NaCl concentration tolerance spanning 2–20% (w/v), with optimal growth observed at 8% NaCl concentration. The cells displayed a spherical morphology and were Gram-positive, non-motile, and devoid of flagella. Biochemical characterization demonstrated positive reactions for both oxidase and catalase activities. Phylogenetic analysis of the 16S rRNA gene sequences revealed that strain Bachu38^T forms an independent branch within the genus <i>Salinicoccus</i>, sharing the highest sequence similarity with <i>S. roseus</i> DSM 5351^T (98.43%) and <i>S. amylolyticus</i> JC304^T (98.29%). The whole genome comprised 2.62 Mb, with a G + C content of 49.5%. When compared with the related strains, the average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values were 71.34–90.99% and 17.20–42.40%, respectively, which were below the suggested species identification threshold (ANI: &lt; 95–96%, dDDH: &lt; 70%) for different prokaryotic species. The respiratory quinone was identified as MK-6, and the cell wall peptidoglycan type was _L-Lys-Gly₅. Fatty acid analysis demonstrated that the major cellular fatty acids were <i>iso</i>-C_15:0 and <i>anteiso</i>-C_15:0, each accounting for more than 10% of the total fatty acid composition. The polar lipid profile consisted of phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), glycolipid (GL), along with two unidentified lipids (designated as UL1 and UL2). Based on polyphasic taxonomic approaches incorporating phenotypic, physiological, and biochemical characteristics, along with comprehensive phylogenetic and genomic analyses, strain Bachu38^T is proposed to represent a novel species of the genus <i>Salinicoccus</i>, named <i>Salinicoccus bachuensis</i> sp. nov., with strain Bachu38^T (= CGMCC NO.28832^T = JCM 37304^T) designated as the type strain.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 5","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143769727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determining the effect of a new truncated CecropinA-Magenin2 (CE-MA) hybrid peptide on the expression of multidrug-resistant (MDR) Mycobacterium tuberculosis efflux genes","authors":"Mozhgan Raigani,&nbsp;Soroush Sardari,&nbsp;Farzam Vaziri,&nbsp;Hasan Ghajavand,&nbsp;Mansour Kargarpour Kamakoli,&nbsp;Mobina Majidian,&nbsp;Mahsa Mazhari,&nbsp;Hamid Madanchi","doi":"10.1007/s00203-025-04314-2","DOIUrl":"10.1007/s00203-025-04314-2","url":null,"abstract":"<div><p>A significant issue in treating bacterial infections is multidrug resistance (MDR) microbes. Drug efflux pumps that reduce cellular drug accumulation are frequently linked to drug resistance. In this study, we set out to determine the effects of CE-MA truncated peptide derivatives against MDR Mycobacterium tuberculosis. Following the assessment of the minimum inhibitory concentrations (MICs) of these peptides against MDR Mycobacterium tuberculosis, a Real-Time PCR was used to examine the expression of six drug efflux pump genes. Next, an MTT assay was performed to test the cytotoxicity of peptides against the A549 cell line. The outcomes demonstrated that CE-MA significantly upregulated gene expression of mmr, and Rv0876c (⩾ 4-fold) than untreated bacteria. Also, under CMt2 stress, significant overexpression of Rv0876c and drrA was seen. However, the results show that upregulation in CMt2-treated bacteria in comparison CE-MA treated bacteria is significantly less for genes tap (<i>P</i> &lt; 0.05), mmr (<i>P</i> &lt; 0.0001), and Rv0876c (<i>P</i> &lt; 0.001). Meanwhile, CMt1 only upregulated the Rv0876c gene and downregulated gene expression of tap, drrA, and mmr. It was also found that all three peptides have no significant effect (<i>P</i> &gt; 0.05) on changing the expression of genes drrC and pstB. Less than 10% of the A549 cell line was susceptible to the toxicity of CMt1 and CMt2 at their MICs range. Our results emphasize the significance of investigating novel peptide-based approaches to combat MDR Mycobacterium tuberculosis and point to these peptides as prospective candidates for additional research.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 5","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143761656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SirA, CsrBC and HilD form in vivo a regulatory cascade that controls the SP1-1 and SPI-2 gene expression when Salmonella Typhimurium is in the intestinal lumen and are required for cecal colonization and liver dissemination in the avian model","authors":"José de Jesús Gómez-Chávez,&nbsp;Jwerlly Tatiana Pico-Rodríguez,&nbsp;Mireya Juárez-Ramírez,&nbsp;Hugo Martínez-Jarquín,&nbsp;Luary C. Martínez-Chavarría","doi":"10.1007/s00203-025-04305-3","DOIUrl":"10.1007/s00203-025-04305-3","url":null,"abstract":"<div><p>When <i>Salmonella</i> Typhimurium grows in LB in vitro, BarA/SirA system induces the expression of CsrB/C, that sequester the regulator CsrA, thus derepressing HilD regulator. HilD activated induces HilA and SsrB expression, central regulators of SPI-1 and SPI-2, respectively. We analyze the in vivo contribution of these genes in 1-day- and 1-week-old chickens infected with a Wild Type strain of <i>S.</i> Typhimurium and the Δ<i>sirA</i>, Δ<i>csrB/C</i> and Δ<i>hilD</i> mutants. CFUs determination in liver and cecum showed that the mutants colonized both organs in lower amounts compared with WT strain in both chicken models and they were affected in the ability to produce histological injuries in these organs. We analyzed whether these genes operate in cascade in vivo and prior to intestinal invasion, by analyzing <i>hilA</i>, <i>ssrAB</i>, <i>hilD</i>, <i>csrB</i> and <i>sirA</i> expression in the cecal contents of chickens inoculated with Wild Type and mutants 120 min after inoculation. Expression of <i>hilA</i> and <i>ssrB</i>, but not <i>csrB</i> and <i>sirA</i>, was decreased in Δ<i>hilD</i> strain. Expression of <i>hilD</i>, <i>hilA</i> and <i>ssrB</i>, but not <i>sirA</i>, was decreased in samples of Δ<i>csrB/C</i>. In SirA absence, expression of all genes was decreased. Our findings demonstrate that SirA, CsrB/C and HilD conform a regulatory cascade in vivo, when <i>Salmonella</i> is in intestinal lumen and this cascade controls the expression of HilA and SsrB prior to intestinal invasion. We also demonstrate that these genes are necessary for the production of lesions during <i>S</i>. Typhimurium infection in chickens.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 5","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00203-025-04305-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143749252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome mining reveals the biosynthetic potential of a novel Lysinibacillus zambalensis sp. nov., isolated from a hyperalkaline spring","authors":"Joyce Amarachi Aja,&nbsp;Lawrence Dave Llorin,&nbsp;Kenji Rowel Q. Lim,&nbsp;Jade Joshua Teodosio,&nbsp;Erwin John Sioson,&nbsp;Ron L. Dy,&nbsp;Carlo A. Arcilla,&nbsp;Doralyn S. Dalisay,&nbsp;Jose Enrico Hizon Lazaro","doi":"10.1007/s00203-025-04316-0","DOIUrl":"10.1007/s00203-025-04316-0","url":null,"abstract":"<div><p>A novel bacterium, designated as strain M3^T, was isolated from a hyperalkaline spring in the Philippines and identified as a new species within the genus <i>Lysinibacillus</i> through 16 S rRNA gene sequence and genomic analyses. Although strain M3^T shared a high 16 S rRNA gene sequence similarity (&gt; 98.7%) with many <i>Lysinibacillus</i> species, the digital DNA-DNA hybridization and orthologous average nucleotide identity values between strain M3^T and its closet relative, <i>Lysinibacillus xylanilyticus</i> DSM 23,493^T, were 41.2% and 90.6%, respectively—both below the established threshold for prokaryotic species delineation. Genome mining of the 5.3 Mbp-draft genome of strain M3^T revealed eight biosynthetic gene clusters, which shared little sequence similarity with characterized clusters, suggesting the potential for encoding novel specialized metabolites. The cells of strain M3^T were Gram-stain-positive, aerobic, rod-shaped, non-motile, and capable of endospore formation. Optimum growth was observed at 30 °C, pH 8.0, and 0.5% (w/v) NaCl. The major respiratory quinone was menaquinone-7, and the predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, and two unknown phospholipids. Its fatty acid profile showed an elevated level of iso-C_15:0, and the peptidoglycan type was determined to be A4<i>α</i> (L-Lys–D-Asp). This study contributes to the growing database and understanding of the genus and aims to help drive future research on the bioactive potential of the genus. <i>Lysinibacillus zambalensis</i> sp. nov. is proposed with strain M3^T as the type strain (= TISTR 10640^T = BIOTECH 10973^T).</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 5","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00203-025-04316-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143749251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Newly developed antibiotics against multidrug-resistant and carbapenem-resistant Gram-negative bacteria: action and resistance mechanisms","authors":"Sena Nur Başaran,&nbsp;Lütfiye Öksüz","doi":"10.1007/s00203-025-04298-z","DOIUrl":"10.1007/s00203-025-04298-z","url":null,"abstract":"<div><p>Antimicrobial resistance stands as one of the most urgent global health concerns in the twenty-first century, with projections suggesting that deaths related to drug-resistant infections could escalate to 10 million by 2050 if proactive measures are not implemented. In intensive care settings, managing infections caused by multidrug-resistant (MDR) Gram-negative bacteria is particularly challenging, posing a significant threat to public health and contributing substantially to both morbidity and mortality. There are numerous studies on the antibiotics responsible for resistance in Gram-negative bacteria, but comprehensive research on resistance mechanisms against new antibiotics is rare. Considering the possibility that antibiotics may no longer be effective in combating diseases, it is crucial to comprehend the problem of emerging resistance to newly developed antibiotics and to implement preventive measures to curb the spread of resistance. Mutations in porins and efflux pumps play a crucial role in antibiotic resistance by altering drug permeability and active efflux. Porin modifications reduce the influx of antibiotics, whereas overexpression of efflux pumps, particularly those in the resistance-nodulation-cell division (RND) family, actively expels antibiotics from bacterial cells, significantly lowering intracellular drug concentrations and leading to treatment failure.</p><p>This review examines the mechanisms of action, resistance profiles, and pharmacokinetic/pharmacodynamic characteristics of newly developed antibiotics designed to combat infections caused by MDR and carbapenem-resistant Gram-negative pathogens. The antibiotics discussed include ceftazidime-avibactam, imipenem-relebactam, ceftolozane-tazobactam, meropenem-vaborbactam, aztreonam-avibactam, delafloxacin, temocillin, plazomicin, cefiderocol, and eravacycline.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 5","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143749254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling multiple copies of MlaC highlights its multifaceted nature","authors":"Ritu Tripathi,&nbsp;Dylan Ayekpam,&nbsp;Shankar Prasad Kanaujia","doi":"10.1007/s00203-025-04308-0","DOIUrl":"10.1007/s00203-025-04308-0","url":null,"abstract":"<div><p>The maintenance of the lipid asymmetry (Mla) system plays a critical role in facilitating the transport of phospholipids between the inner and outer membranes of the Gram-negative bacteria. In <i>E. coli</i>, the system consists of six proteins: MlaA-OmpF/C complex (outer membrane), MlaC (periplasm), and MlaFEDB complex (inner membrane). Despite extensive research on the core proteins (MlaFED) of the Mla system, the occurrence of Mla components like MlaA, MlaB, and MlaC in diderm remains uncertain. Therefore, this gap presents a significant opportunity for further investigation, particularly regarding MlaC, which serves as the sole mobile component of the Mla system. This has led to the identification of multiple copies of MlaC in 63 distinct genera of Proteobacteria and related phyla. Interestingly, amongst these genera, the genetic arrangements of the <i>mla</i> operon were observed to be varying and, thus, were further categorized into four distinct groups. The variations among the genetic organization of the <i>mla</i> operons suggest their evolution through various processes, such as duplications, losses, rearrangements, and fusions. Further, the results of this study highlight the MlaC’s substrate promiscuity, illuminating new avenues for the Mla system.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 5","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143749253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cra-controlled antisense RNA-downregulation of isocitrate dehydrogenase in Escherichia coli","authors":"Jisha Elias,&nbsp;Vikas Sharma,&nbsp;G. Archana,&nbsp;G. Naresh Kumar","doi":"10.1007/s00203-025-04290-7","DOIUrl":"10.1007/s00203-025-04290-7","url":null,"abstract":"<div><p>Catabolite repressor activator (Cra) protein (formerly called FruR) found in <i>E.</i> <i>coli</i> is known to regulate the expression of many genes positively and negatively in response to the intracellular levels of fructose-1-phosphate (F-1-P) and fructose-1,6-bisphopahate (F-1,6-bisP). In this paper, we report synthesis and characterization of a conditionally expressed antisense RNA corresponding to 101 bp of isocitrate dehydrogenase (<i>icd)</i> gene (as-<i>icd</i>) under Cra (FruR) responsive promoter <i>fruB</i> (P_<i>fruB</i> as-<i>icd</i> construct denoted as pVS2K3) in <i>E.</i> <i>coli</i> K-12 derivative (DH5α) and <i>E.</i> <i>coli</i> B derivative (BL21) strains. Previous studies have shown that ICD mutants accumulated citrate intracellularly but failed to grow on glucose in absence of glutamate. Hence, a conditional downregulation of <i>icd</i> gene could be helpful in overcoming this lethality and also aid in understanding the flux towards citrate accumulation. Effect of pVS2K3 construct was monitored in <i>E.</i> <i>coli</i> DH5α and <i>E.</i> <i>coli</i> BL21 during growth on carbon sources wherein the fruB promoter is active (glucose) or repressed (glycerol). A 3-to 4-fold decrease in <b>ICDH</b> activity was observed in <i>E.</i> <i>coli</i> DH5α expressing pVS2K3 on glucose but no change in ICDH activity was observed in <i>E.</i> <i>coli</i> BL21 expressing pVS2K3 on glucose. This alteration could be attributed to the anomalous Cra regulation seen in <i>E.</i> <i>coli</i> B strain which could be a crucial factor while choosing P_<i>fru</i>B promoter for expression studies.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 5","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143740630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial enhanced oil recovery: process perspectives, challenges, and advanced technologies for its efficient applications and feasibility","authors":"Ankita Das,&nbsp;Nandita Das,&nbsp;Prisha Pandey,&nbsp;Piyush Pandey","doi":"10.1007/s00203-025-04307-1","DOIUrl":"10.1007/s00203-025-04307-1","url":null,"abstract":"<div><p>The depletion of crude oil resources, coupled with increasing energy demands, underscores the urgency for innovative recovery strategies. Despite traditional oil recovery operations, a substantial portion of residual oil (~70%) persists within the intricate capillary networks of oil reservoirs’ pore spaces. Microbial Enhanced Oil Recovery (MEOR) is an ecofriendly and cost-effective tertiary oil recovery method that offers a sustainable approach by utilizing microorganisms and their metabolites to extract this residual oil from mature or depleted reservoirs. MEOR is an emerging process gaining global attention, with numerous research studies and field trials underway worldwide. This review explores microbial strategies for modifying reservoir rheological properties, underscores the significance of microbial physiology and diversity, and examines omics technologies for deciphering microbial mechanisms to enhance the efficiency of MEOR. Cutting-edge advancements, including genetically modified microbes, enzyme-based techniques, and nanotechnology, have been discussed as potential enhancers of MEOR efficiency. The economic feasibility and integration of MEOR with Carbon Capture and Utilization (CCUS) are also assessed, emphasizing its role amid declining conventional oil production. Further, the economic and application feasibility along with patents related to MEOR technologies is presented which underscores its commercial viability. By addressing challenges and proposing solutions, this review provides a comprehensive outlook on MEOR’s future, aiming to guide research and development for its successful application in sustainable oil recovery.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 5","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143740631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}