Archives of Microbiology最新文献

{"title":"Viral tropism in plants, reproductive tissues, and seeds","authors":"Leandro Alberto Núñez-Muñoz,&nbsp;Berenice Calderón-Pérez,&nbsp;Roberto Ruiz-Medrano,&nbsp;Beatriz Xoconostle-Cázares,&nbsp;Rodolfo de la Torre-Almaraz","doi":"10.1007/s00203-025-04353-9","DOIUrl":"10.1007/s00203-025-04353-9","url":null,"abstract":"<div><p>Plant viral tropism refers to virus ability for infecting and replicating within specific cell types, tissues or hosts. Plant viral tropism is shaped by the absence of specific membrane-associated viral receptors and the supracellular nature of viral transport through plasmodesmata and vascular tissues. This review focuses on the molecular and cellular determinants of plant viral tropism, including modifications in plasmodesmal permeability, host-mediated RNA silencing, and tissue-specific viral protein localization. We discuss how certain viruses target reproductive organs, meristems, and seeds, overcoming antiviral barriers to establish persistent infections. Additionally, we explore the role of host factors in shaping viral distribution. Advances in super-resolution microscopy, single-cell transcriptomics, and proteomics have significantly expanded our ability to dissect virus-host interactions at the nanoscale, uncovering new mechanisms of viral accumulation. Understanding these processes is essential not only for improving crop resistance and designing integrated disease management strategies, but also for repurposing plant viruses as tools for targeted delivery and biotechnological applications.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 7","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00203-025-04353-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144117669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unravelling fungal genome editing revolution: pathological and biotechnological application aspects","authors":"Abdallah M. A. Hassane,&nbsp;Marwa Obiedallah,&nbsp;Javad Karimi,&nbsp;Sadat M. R. Khattab,&nbsp;Hussein R. Hussein,&nbsp;Youssef Abo-Dahab,&nbsp;Adel Eltoukhy,&nbsp;Nageh F. Abo-Dahab,&nbsp;Mohamed E. Abouelela","doi":"10.1007/s00203-025-04360-w","DOIUrl":"10.1007/s00203-025-04360-w","url":null,"abstract":"<div><p>Fungi represent a broad and evolutionarily unique group within the eukaryotic domain, characterized by extensive ecological adaptability and metabolic versatility. Their inherent biological intricacy is evident in the diverse and dynamic relationships they establish with various hosts and environmental niches. Notably, fungi are integral to disease processes and a wide array of biotechnological innovations, highlighting their significance in medical, agricultural, and industrial domains. Recent advances in genetic engineering have revolutionized fungal research, with CRISPR/Cas emerging as the most potent and versatile genome editing platform. This technology enables precise manipulation of fungal genomes, from silencing efflux pump genes in <i>Candida albicans</i> (enhancing antifungal susceptibility) to targeting virulence-associated sirtuins in <i>Aspergillus fumigatus</i> (attenuating pathogenicity). Its applications span gene overexpression, multiplexed mutagenesis, and secondary metabolite induction, proving transformative for disease management and biotechnological innovation. CRISPR/Cas9’s advantages—unmatched precision, cost-effectiveness, and therapeutic potential—are tempered by challenges like off-target effects, ethical dilemmas, and regulatory gaps. Integrating nanoparticle delivery systems and multi-omics approaches may overcome technical barriers, but responsible innovation requires addressing these limitations. CRISPR-driven fungal genome editing promises to redefine solutions for drug-resistant infections, sustainable bioproduction, and beyond as the field evolves. In conclusion, genome editing technologies have enhanced our capacity to dissect fungal biology and expanded fungi’s practical applications across various scientific and industrial domains. Continued innovation in this field promises to unlock the vast potential of fungal systems further, enabling more profound understanding and transformative biotechnological progress.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 7","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144108660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"One-step narrow-thermal-cycling strand exchange amplification for sensitive detection of porcine reproductive and respiratory syndrome virus","authors":"Linlin Zhuang,&nbsp;Chunlei Song,&nbsp;Li Sun,&nbsp;Haiqiang Xie,&nbsp;Liqun Wang,&nbsp;Qingxin Liu,&nbsp;Hongjing Shi,&nbsp;Jianbo Yang,&nbsp;Qiuping Shen","doi":"10.1007/s00203-025-04351-x","DOIUrl":"10.1007/s00203-025-04351-x","url":null,"abstract":"<div><p>Porcine reproductive and respiratory syndrome (PRRS), which is primarily characterized by respiratory and reproductive dysfunction, is an epidemic disease caused by porcine reproductive and respiratory syndrome virus (PRRSV) that has the potential to economically devastate the global swine industry. Rapid and accurate detection of PRRSV is critical for effective control of PRRS in swine. In this study, a novel one-step, highly sensitive and specific accelerated strand exchange amplification (ASEA) method for the detection of PRRSV was developed. The detection limit of the ASEA method was determined to be 6 copies µL^–1 of PRRSV RNA reference material or PRRSV in spiked swine blood. The ASEA method demonstrated the capacity to discern the currently circulating PRRSV genotypes without cross-reactivity with other porcine-derived pathogens that manifest similar clinical signs. The ASEA method exhibited a detection time of 35 min, and its clinical applicability was validated through the analysis of 5 inactivated blood samples and 62 clinical samples. The method’s potential extends beyond the diagnosis of PRRSV, as it can also be applied to the rapid diagnosis of other RNA pathogens. This capacity is expected to make significant contributions to future epidemic prevention and surveillance efforts.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 7","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144108445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glucose starvation induces tau phosphorylation leading to cellular stress response in fission yeast","authors":"Merve Yılmazer,&nbsp;Aslıhan Şengelen,&nbsp;Yunus Aksüt,&nbsp;Bedia Palabıyık,&nbsp;Evren Önay-Uçar,&nbsp;Semian Karaer Uzuner","doi":"10.1007/s00203-025-04350-y","DOIUrl":"10.1007/s00203-025-04350-y","url":null,"abstract":"<div><p>Misfolded tau proteins and their accumulation cause many neurodegenerative diseases named tauopathies. While phosphorylation is required for tau protein activity, hyperphosphorylation leads to pathological conditions. Previous reports have shown that glucose deprivation might influence tau protein formation and phosphorylation in vivo, though its effect on cellular stress pathways in a yeast model has not been documented. In this study, we examined the various cellular processes, including oxidative and ER stress responses, glucose metabolism, autophagy, 20 S proteasomal activity, and glucose consumption in <i>Schizosaccharomyces pombe</i> cells heterologously expressing the human <i>MAPT</i> gene, which we obtained in our previous study. We observed increased levels of <i>MAPT</i> gene expression, phosphorylated tau protein (sites at Thr181, Thr231, and Ser396), and phosphorylated GSK-3β (site at Tyr216; contributes to tau phosphorylation) under glucose starvation conditions. The presence of tau protein led to increased expression levels of genes related to oxidative stress response and ER stress in fission yeast. Glucose-starved yeast expressing tau showed higher proteasomal activity and autophagy than control cells in normal glucose conditions. Additionally, cells containing tau protein exhibited higher glucose consumption under nutrient starvation conditions than those lacking tau. These findings indicate a possible relationship between increased tau protein phosphorylation and glucose metabolism, supporting the connection among tauopathies, poorly regulated blood sugar, and diabetes; thus, this provides initial evidence that <i>S. pombe</i> yeast can serve as a model for research in this area.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 7","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144084868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Current status and future prospects of microalgae-based degradation of spent lubricant oil hydrocarbon towards environmental sustainability: a mini review and bibliometric analysis","authors":"Stella B. Eregie,&nbsp;Isaac A. Sanusi,&nbsp;Olaniran O. Ademola","doi":"10.1007/s00203-025-04332-0","DOIUrl":"10.1007/s00203-025-04332-0","url":null,"abstract":"<div><p>The biodegradation of spent oil waste (SOW) using bacteria and fungi has been actively researched over the years. Only recently has the use of microalgae for the treatment of SOW attracted significant attention. This review aims to highlight the biodegradative capabilities of microalgae as well as provide a comprehensive bibliometric analysis to assess current research activities and trends in microalgae-based biodegradation of SOW. The bibliographic data exported from Dimensions database was analyzed using VOSviewer, focusing on various aspects such as document types, publications, subject categories, sources, countries, authors, organizations, and cited articles. The results obtained showed a remarkable increase (80.23%) in the number of article publications from 2005 to 2023 in this field of research. China (887 publications), Environmental Science (3571 publications), Bioresource Technology (249 publications) and Harbin Institute of Technology (72 publications), were the most productive country, subject category, journal, and organization, respectively, publishing articles in this field of research. The review also discussed SOW hydrocarbons ranging from alkanes, aromatic compounds to polychlorinated compounds and the mechanism of degradation of these compounds by microalgae. Overall, the review provided useful insight on microalgae SOW degradation, current research direction and the prospect of using microalgae in environmental remediation and sustainability.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 7","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00203-025-04332-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144090947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Generation and validation of a versatile inducible multiplex CRISPRi system to examine bacterial regulation in the Euprymna-Vibrio fischeri symbiosis","authors":"Brian Lynn Pipes,&nbsp;Michele Kiyoko Nishiguchi","doi":"10.1007/s00203-025-04354-8","DOIUrl":"10.1007/s00203-025-04354-8","url":null,"abstract":"<div><p>The <i>Vibrio fischeri</i>—<i>Euprymna scolopes</i> symbiosis has become a powerful animal—microbe model system to examine the genetic underpinnings of symbiont development and regulation. Although there has been a number of elegant bacterial genetic technologies developed to examine this symbiosis, there is still a need to develop more sophisticated methodologies to better understand complex regulatory pathways that lie within the association. Therefore, we have developed a suite of CRISPR interference (CRISPRi) vectors for inducible repression of specific <i>V. fischeri</i> genes associated with symbiotic competence. The suite utilizes both Tn7-integrating and shuttle vector plasmids that allow for inducible expression of CRISPRi dCas9 protein along with single-guide RNAs (sgRNA) modules. We validated this CRISPRi tool suite by targeting both exogenous (an introduced mRFP reporter) and endogenous genes (<i>luxC</i> in the bioluminescence producing <i>lux</i> operon, and f<i>lrA</i>, the major regulatory gene controlling flagella production). The suite includes shuttle vectors expressing both single and multiple sgRNAs complementary to the non-template strand of multiple targeted genetic loci, which were effective in inducible gene repression, with significant reductions in targeted gene expression levels. <i>V. fischeri</i> cells harboring a version of this system targeting the <i>luxC</i> gene and suppressing the production of luminescence were used to experimentally validate the hypothesis that continuous luminescence must be produced by the symbiont in order to maintain the symbiosis at time points longer than the known 24-h limit. This robust new CRISPRi genetic toolset has broad utility and will enhance the study of <i>V. fischeri</i> genes, bypassing the need for gene disruptions by standard techniques of allelic knockout-complementation-exchange and the ability to visualize symbiotic regulation in vivo.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 7","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00203-025-04354-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144074109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RsmG methylation of 16S rRNA affects the function of ribosomal protein uS12","authors":"Trevor W. Bell,&nbsp;Rowan M. Turner,&nbsp;Amanda M. Merryman,&nbsp;Juliana J. Joseph,&nbsp;Steven T. Gregory,&nbsp;Michael O’Connor","doi":"10.1007/s00203-025-04349-5","DOIUrl":"10.1007/s00203-025-04349-5","url":null,"abstract":"<div><p>The RsmG methyltransferase modifies G527 in bacterial 16S rRNA and its inactivation confers low level streptomycin resistance. In contrast, high level streptomycin resistance typically requires specific alterations in ribosomal protein uS12 or 16S rRNA. Here, we have asked if <i>rsmG</i> inactivation alters the phenotypes of any of a collection of randomly-generated <i>Escherichia coli</i> uS12 mutants. While several uS12 mutants show moderately increased resistance to streptomycin when <i>rsmG</i> is inactivated (MIC = 10–40 µg/ml), a uS12 R85H/<i>rsmG</i>-inactivated strain uniquely displays very high resistance (MIC &gt; 1,024 µg/ml). Additional genetic selections showed that <i>rsmG</i> null mutations combined with specific alterations in uS12 can generate streptomycin-dependence, or pseudo-dependence, in addition to resistance. Moreover, growth of several of these mutants on high concentrations of streptomycin is conditional on <i>rsmG</i> inactivation. Thus, loss of m⁷G527 methylation affects the streptomycin phenotypes of distinct uS12 mutants and identifies an additional route to high-level streptomycin resistance in bacteria.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 7","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144073616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endophytes of Zingiberaceae: distribution and bioactivity of their bioactive metabolites","authors":"Nor Hazwani Mohd Hasali,&nbsp;Muhammad Shahbaz,&nbsp;Jaya Seelan Sathiya Seelan,&nbsp;Nazirul Azam Ibrahim,&nbsp;Christophe Wiart,&nbsp;Nivaarani Arumugam,&nbsp;Mohammed Abdus Salam,&nbsp;Muneeb M. Musthafa,&nbsp;Suganthi Appalasamy","doi":"10.1007/s00203-025-04335-x","DOIUrl":"10.1007/s00203-025-04335-x","url":null,"abstract":"<div><p>The endophytes associated with the Zingiberaceae family have demonstrated remarkable potential in various biological activities through their bioactive compounds that are useful for both medical and agricultural purposes. Additionally, isolated secondary metabolites from symbiotic microbes associated with this family of plants have shown promising results in various biological activities such as antifungal, anticancer, antidiabetic, anti-inflammatory, and antibacterial. This review focuses on the bioactive metabolites of Zingiberaceae endophytes with their potential biological activities against different pathogens as well as the production of phytohormones that promote plant growth. Furthermore, bibliometric analysis revealed the current key trends and collaborative works in this field. Additionally, the bibliometric analysis also explored a total of 75 relevant publications from the Scopus database that India is the most contributing country in this field with 38.7% of the total reported research work. The bibliometric approach helps uncover new research gaps by identifying emerging trends, underexplored topics, and collaborative networks, providing insights into areas that require further investigation and development. Notably, <i>Streptomyces</i> spp. have been commonly reported as potent endophytes, generating bioactive substances such as Vanillin and Resacetophenone with strong antibacterial activities. Significant knowledge gaps still exist, and with evolving therapeutic potential, this could offer a wide opportunity for new studies to emerge.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 6","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143938312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the oral bacteria-oral lichen planus connection: mechanisms, clinical implications and future directions","authors":"Wei-long Zhang,&nbsp;Lian-pin Yu,&nbsp;Wei Zhou,&nbsp;Xue Wang,&nbsp;Juan Du","doi":"10.1007/s00203-025-04342-y","DOIUrl":"10.1007/s00203-025-04342-y","url":null,"abstract":"<div><p>Oral lichen planus (OLP) is a prevalent T-cell mediated inflammatory-immune disease with uncertain etiology. Recently, there is emerging evidence suggesting that oral bacteria may exert a prominent role in the onset and development of OLP. They might promote the initiation and progression of OLP by disrupting the oral epithelia, invading the lamina propria, stimulating pro-inflammatory cytokines production and inducing immune dysfunction. In this review, we will focus on the possible mechanisms of oral bacteria contributing to occurrence and development of OLP, and provide new insights into the bacteria-related diagnosis, prevention and treatment strategies for OLP.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 6","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143938417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impacts of PAR and UV radiation on diurnal photosynthesis performance, pigment composition, and antioxidant function of the hot-spring cyanobacterium Nostoc sp. strain VKB02","authors":"Megha Jaiswal,&nbsp;Nasreen Amin,&nbsp;Vinod K. Kannaujiya","doi":"10.1007/s00203-025-04338-8","DOIUrl":"10.1007/s00203-025-04338-8","url":null,"abstract":"<div><p>Diurnal oscillations are 24 h clock, that synchronize organisms biological functions based on the daily environmental fluctuation. Continuous increase in ultraviolet radiation have been shown to affect the biological clock of cyanobacteria. The present investigation deals with the 12/12 h of light/dark effects of PAR and PAB (PAR + UVA + UVB) irradiations on ecophysiology and defense management of a hot-spring cyanobacterium <i>Nostoc</i> sp. strain VKB02. The alternative L/D exposure of PAR showed increase in growth and pigment compositions. However, PAB radiation has significantly decreased within the same parameters after the L1 phase except for carotenoid and APC, while PE and PC recovered till the D2 phase corresponding to the counter light phase. The pigments destruction also resulted in Chl <i>a</i> fluorescence (F_v/F_m, F_v׳/F_m׳, Y(II), rETR) emission decline. In addition, PAB exposure accelerated free radicals generation with induced protein oxidation (RCG) and antioxidative enzymes (SOD, POD, CAT) as counteract defense during the light phase. The overall circadian regime facilitated the resynthesis fate of pigment-protein complexes and the mitigation of the high level of ROS production. This result suggests the unique survival strategy of the hot-spring cyanobacterium against ultraviolet radiation in a diurnal manner. This study also offers a deep understanding of the diurnal eco-physiological and biochemical responses of the cyanobacterium for the advancement in sustainable agricultural production with lower input in variable climate.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 6","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143938418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}