Archives of Microbiology最新文献_第2页

Statistical factorial design for optimum reduction of tellurite and production of tellurium nanostructure by a novel strain Phytobacter diazotrophicus Te1 新菌株重氮营养菌Te1还原碲及产碲纳米结构的统计因子设计

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2025-04-15 DOI: 10.1007/s00203-025-04313-3

Alaa A. Abdel-Samad, Mohamed Ismaeil, Einas H. El-Shatoury, Ali M. Saeed

{"title":"Statistical factorial design for optimum reduction of tellurite and production of tellurium nanostructure by a novel strain Phytobacter diazotrophicus Te1","authors":"Alaa A. Abdel-Samad, Mohamed Ismaeil, Einas H. El-Shatoury, Ali M. Saeed","doi":"10.1007/s00203-025-04313-3","DOIUrl":"10.1007/s00203-025-04313-3","url":null,"abstract":"<div><p>A tellurite-reducing isolate (Te1) was recovered from a soil sample receiving industrial effluents from Ismailia Canal, Egypt. The isolate exhibited dark black colonies when grown on solid medium containing potassium tellurite, which indicated the reduction of tellurite to black tellurium. The isolate was identified using 16S rRNA gene sequencing and was submitted to GenBank as <i>Phytobacter diazotrophicus</i> strain Te1 (PP724698). The tellurite reduction percentage was 96.5% ± 0.354%. Moreover, energy-dispersive X-ray (EDX) analysis confirmed the presence of tellurium nanostructure, with a 3.7 keV absorption peak along with phosphorus, sulfur, and oxygen, revealing a complex biogenic nature. Fourier-transform infrared (FTIR) spectroscopy identified distinct absorption peaks within the 400–4000 cm<sup>−1</sup> range, corresponding to various vibrational modes of chemical bonds, including those of lipids, proteins, polysaccharides, and free radicals. X-ray diffraction (XRD) analysis highlighted the nanoscale crystalline structure of the material, with broad peaks confirming limited crystallite size and structural disorder, and revealed tellurium peaks on a hexagonal phase at 2-theta values of 27.36°, 38.19° and 40.20°. According to the results of the response optimizer and the subsequent validation experiments, complete reduction of tellurium was achieved at a medium pH of 6.8, incubation temperature of 33.5 °C, tellurite concentration of 1375 μM, and agitation speed of 110 rpm for 96 h. Black Te nanostructure was visible intracellularly and extracellularly upon examination using the transmission electron microscope. To the best of the authors’ knowledge, this is the first report of tellurite reduction by <i>Phytobacter diazotrophicus</i>.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 6","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143835699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-wide identification and expression analysis of epigenetic regulator gene families in the medicinal mushroom Ganoderma lucidum 药用蘑菇灵芝中表观遗传调控基因家族的全基因组鉴定和表达分析

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2025-04-15 DOI: 10.1007/s00203-025-04326-y

Precious Bondzie-Quaye, Frederick Leo Sossah, Mohammed Sharif Swallah, Monia Ravelonandrasana Fetisoa, Mona Alrasheed Bashir, Qing Huang

{"title":"Genome-wide identification and expression analysis of epigenetic regulator gene families in the medicinal mushroom Ganoderma lucidum","authors":"Precious Bondzie-Quaye, Frederick Leo Sossah, Mohammed Sharif Swallah, Monia Ravelonandrasana Fetisoa, Mona Alrasheed Bashir, Qing Huang","doi":"10.1007/s00203-025-04326-y","DOIUrl":"10.1007/s00203-025-04326-y","url":null,"abstract":"<div><p>Epigenetic regulator (ER) genes, crucial for fungal growth and development, remain largely unexplored in <i>Ganoderma lucidum</i>, a medicinal mushroom valued for its bioactive compounds. This study identified 81 ER genes in <i>G. lucidum</i>, distributed across 12 chromosomes and classified into six families: 3 chromatin remodelers, 4 DNA methyltransferases, 7 histone acetyltransferases, 22 histone deacetylases, 23 histone methyltransferases, and 22 histone demethyltransferases. Comparative and phylogenetic analyses with other species revealed conserved orthologs and species-specific clusters. Gene duplication analysis suggested whole-genome duplication expanded ER gene families, primarily histone demethyltransferases under purifying selection. Additionally, gene structure, motif, and domain analyses revealed family-specific intron/exon organization and conserved domains. Transcriptome profiling across four developmental stages (mycelium, primordia, young and mature fruiting body) revealed dynamic stage-specific expression patterns, suggesting their developmental significance. The result of qRT-PCR validated the expression patterns for 18 ER genes, laying foundation for future research exploring epigenetic regulation in fungal development and bioactive compound production.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 6","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143835697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Staphylococcus hominis as a source of antimicrobial peptides: identification of a new peptide with potential antimicrobial properties using in silico approach 人型葡萄球菌作为抗菌肽的来源：一种具有潜在抗菌特性的新肽的鉴定

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2025-04-11 DOI: 10.1007/s00203-025-04323-1

Mohammed Walid Chemao-Elfihri, Mohammed Hakmi, Hanane Abbou, Souad Kartti, Elmostafa El Fahime, Lahcen Belyamani, Saber Boutayeb

{"title":"Staphylococcus hominis as a source of antimicrobial peptides: identification of a new peptide with potential antimicrobial properties using in silico approach","authors":"Mohammed Walid Chemao-Elfihri, Mohammed Hakmi, Hanane Abbou, Souad Kartti, Elmostafa El Fahime, Lahcen Belyamani, Saber Boutayeb","doi":"10.1007/s00203-025-04323-1","DOIUrl":"10.1007/s00203-025-04323-1","url":null,"abstract":"<div><p>The rapid progression of antimicrobial resistance, fueled by the excessive use of antibiotics, has become a major public health concern. Among the pathogens contributing to this crisis, <i>Staphylococcus aureus</i> stands out as a significant therapeutic challenge, especially with the rise of resistant strains like Methicillin-Resistant <i>S. aureus</i> (MRSA). In this context, antimicrobial peptides (AMPs) emerge as a promising alternative, thanks to their unique mechanisms of action. Exploring the genomes of species such as <i>Staphylococcus hominis</i>, known for producing AMPs effective against <i>S. aureus</i>, offers promising opportunities for discovering novel therapeutic agents. In this study, Average Nucleotide Identity (ANI) combined with phylogenetic analysis identified a potential emerging subspecies of <i>Staphylococcus hominis</i>. The core genome analysis led to the identification of a potential antimicrobial peptide. The peptide model simulated with the <i>S. aureus</i> membrane model in molecular dynamics revealed that it interacts primarily with the lipids head groups, leading to an overall rigidification of the bacterial membrane.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 5","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143818251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Environmental fate of antibiotic resistance genes in livestock farming 畜牧业中抗生素抗性基因的环境命运

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2025-04-11 DOI: 10.1007/s00203-025-04320-4

Jiali Sun, Xiaoqi Wang, Yuanjie He, Mengting Han, Mei Li, Siyue Wang, Jia Chen, Qiang Zhang, Baowei Yang

{"title":"Environmental fate of antibiotic resistance genes in livestock farming","authors":"Jiali Sun, Xiaoqi Wang, Yuanjie He, Mengting Han, Mei Li, Siyue Wang, Jia Chen, Qiang Zhang, Baowei Yang","doi":"10.1007/s00203-025-04320-4","DOIUrl":"10.1007/s00203-025-04320-4","url":null,"abstract":"<div><p>As emerging environmental pollutants, antibiotic resistance genes (ARGs) are prevalent in livestock farms and their surrounding environments. Although existing studies have focused on ARGs in specific environmental media, comprehensive research on ARGs within farming environments and their adjacent areas remains scarce. This review explores the sources, pollution status, and transmission pathways of ARGs from farms to the surrounding environment. Drawing on the “One Health” concept, it also discusses the potential risks of ARGs transmission from animals to human pathogens and the resulting impact on human health. Our findings suggest that the emergence of ARGs in livestock farming environments primarily results from intrinsic resistance and genetic mutations, while their spread is largely driven by horizontal gene transfer. The distribution of ARGs varies according to the type of resistance genes, seasonal changes, and the medium in which they are present. ARGs are disseminated into the surrounding environment via pathways such as manure application, wastewater discharge, and aerosol diffusion. They may be absorbed by humans, accumulating in the intestinal microbiota and subsequently affecting human health. The spread of ARGs is influenced by the interplay of microbial communities, antibiotics, heavy metals, emerging pollutants, and environmental factors. Additionally, we have outlined three control strategies: reducing the emergence of ARGs at the source, controlling their spread, and minimizing human exposure. This article provides a theoretical framework and scientific guidance for understanding the cross-media migration of microbial resistance in livestock farming environments.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 5","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143818250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the potential of bacterial-derived EVs for targeted enzyme replacement therapy: mechanisms, applications, and future directions 探索细菌源性ev用于靶向酶替代治疗的潜力：机制、应用和未来方向

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2025-04-10 DOI: 10.1007/s00203-025-04294-3

Mohanakrishna Raja, Karthikeyan Ramamurthy, Gokul Sudhakaran, Ajay Guru, Jesu Arockiaraj

{"title":"Exploring the potential of bacterial-derived EVs for targeted enzyme replacement therapy: mechanisms, applications, and future directions","authors":"Mohanakrishna Raja, Karthikeyan Ramamurthy, Gokul Sudhakaran, Ajay Guru, Jesu Arockiaraj","doi":"10.1007/s00203-025-04294-3","DOIUrl":"10.1007/s00203-025-04294-3","url":null,"abstract":"<div><p>Extracellular vesicles (EVs) are membrane-bound vesicles produced by cells which promote intercellular communication by delivering different contents such as DNA, RNA, and proteins. These vesicles, nano-sized and released into the extracellular space, are present everywhere under both normal and pathological conditions. Probiotic-derived EVs can serve as nanocarriers for therapeutic cargo, particularly in enzyme replacement therapy (ERT). Traditional ERT for lysosomal storage diseases (LSDs) faces significant challenges, including the inability of enzymes to cross the blood–brain barrier (BBB) and their susceptibility to degradation. Studies show EVs can transport enzyme cargoes across the BBB, accurately delivering them to tissues affected by LSDs. Probiotic EVs also possess immunomodulatory properties, providing therapeutic benefits in inflammatory conditions. However, their potential for delivering deficient enzymes in LSDs remains unclear. This review discusses using probiotic EVs in ERT for targeted enzyme delivery to treat LSDs more efficiently than other exosomes. This novel strategy minimizes off-target delivery and enhances immunomodulatory effects, making it more advantageous than live probiotic bacteria. Probiotic EVs show promise for therapeutic approaches, especially in treating LSDs and inflammatory diseases, by modulating immune responses and delivering enzymes across biological barriers like the BBB. Future research should optimize production, engineer targeted therapies, and confirm safety and efficacy through clinical trials. Expanding studies to include diverse probiotic strains could uncover new therapeutic applications, enhancing their versatility and effectiveness.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 5","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143809098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Applications of endophytic fungi in plant disease control 内生真菌在植物病害控制中的应用

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2025-04-09 DOI: 10.1007/s00203-025-04303-5

Yang Zhang, Jingru Liang, Jiajie Shi, Wenhui Yuan, Xintao Li, Changhong Ding

{"title":"Applications of endophytic fungi in plant disease control","authors":"Yang Zhang, Jingru Liang, Jiajie Shi, Wenhui Yuan, Xintao Li, Changhong Ding","doi":"10.1007/s00203-025-04303-5","DOIUrl":"10.1007/s00203-025-04303-5","url":null,"abstract":"<div><p>Diseases caused by pathogenic microorganisms (bacteria, fungi, and viruses) have resulted in the quality and yield of crops, which has seriously affected the development of the agricultural economy. The prolonged use of chemical fungicides for prevention and control can lead to environmental pollution, hindering the sustainable development of safe and eco-friendly agriculture while also promoting the resistance of pathogenic microorganisms. Nevertheless, non–pathogenic endophytic fungi that form symbiotic relationships with plants still exhibit significant antagonistic effects on pathogenic microorganisms, even in small concentrations. These fungi pose no threat to human health and are highly beneficial to the ecological environment, making them an ideal alternative to chemical fungicides. They are increasingly being recognized and have been subjected to comprehensive research. Based on this, this article summarizes the types of endophytic fungi with biocontrol effects in recent years. It focuses on elucidating the mechanisms of their biocontrol from physiological and molecular perspectives. In addition, the application and development challenges of biocontrol agents (BCAs) derived from these fungi are also discussed, including difficulties in elucidating their mechanisms of action during research and development, challenges in strain selection and improvement, difficulties in controlling environmental adaptability, and stringent storage conditions. The aim is to develop more effective endophytic fungi as emerging biocontrol resources for agricultural production.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 5","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143809285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing killer yeast system: from molecular insight to real world biocontrol solution 利用杀手酵母系统：从分子洞察到现实世界的生物控制解决方案

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2025-04-08 DOI: 10.1007/s00203-025-04318-y

Prabhsangam Kaur Dhillon, Manpreet Kaur, Sukesh Chander Sharma, Akhtar Mahmood

{"title":"Harnessing killer yeast system: from molecular insight to real world biocontrol solution","authors":"Prabhsangam Kaur Dhillon, Manpreet Kaur, Sukesh Chander Sharma, Akhtar Mahmood","doi":"10.1007/s00203-025-04318-y","DOIUrl":"10.1007/s00203-025-04318-y","url":null,"abstract":"<div><p>The biocontrol mechanisms of yeasts are gaining global attention as a vital response to the rising threat of antimicrobial resistance in emerging pathogens. With their diverse genetic traits, yeasts present potent antagonistic capabilities that have extensive applications across various sectors. This review examines the fundamental mechanisms and molecular bases of yeast antagonism, emphasizing innovative screening techniques for identifying effective strains. Key molecular methods, including gene identification, sequence analysis, and expression studies, reveal insights into their modes of action, such as nutrient competition, mycocin production, and volatile organic compound release. While the potential of antagonistic yeasts spans crop protection, food safety, and disease prevention, their commercial availability remains limited. To foster adoption, there is an urgent need for advanced screening methods and deeper molecular understanding. Future research should prioritize the optimization and commercialization of yeast-based biocontrol products, effectively bridging the gap between scientific discovery and practical application in combating harmful pathogens.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 5","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143793137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Computational prediction of Homo sapiens–Candida albicans protein–protein interactions reveal key virulence factors using dual RNA-Seq data analysis 利用双RNA-Seq数据分析计算预测智人-白色念珠菌蛋白-蛋白相互作用揭示关键毒力因子

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2025-04-06 DOI: 10.1007/s00203-025-04312-4

Ekjot Kaur, Vishal Acharya

{"title":"Computational prediction of Homo sapiens–Candida albicans protein–protein interactions reveal key virulence factors using dual RNA-Seq data analysis","authors":"Ekjot Kaur, Vishal Acharya","doi":"10.1007/s00203-025-04312-4","DOIUrl":"10.1007/s00203-025-04312-4","url":null,"abstract":"<div><p>A prevalent pathobiont, <i>Candida albicans</i>, accounts for approximately 70% of fungal infections worldwide owing to its virulence traits that culminate in devastating fatalities within healthcare facilities. Protein–protein interactions (PPIs) between <i>Homo sapiens</i> and <i>C. albicans</i> play a pivotal role in infection and disease progression. Additionally, scarcity of information on <i>H. sapiens</i>–<i>C. albicans</i> protein–protein interactions makes it difficult to understand the molecular mechanisms underlying infection and host immune responses. Investigating these PPIs can provide crucial insights into host–pathogen relationships and facilitate the development of novel therapeutic interventions. To address this challenge, we utilized computational techniques based on homology and domain to project 56,515 human-fungal pathogen protein–protein interactions (HF-PPIs) involving 6830 human and 486 <i>C. albicans</i> proteins. We have identified 16 key virulence factors of <i>C. albicans</i>, including SOD1, ERG10, GFA1, and VPS4, as potential therapeutic targets. As evidenced by dual RNA-Seq data acquired at various stages of infection such as 15, 30, 60, 120, and 240 min, these fungal genes interact with down-regulated human immunomodulatory genes specifically, ADRM1, DAXX, RYBP, SGTA, and SRGN. In addition to their intrinsically disordered regions, these human genes are particularly susceptible to fungal manipulation. Through the identification of experimentally validated virulence factors and their interaction partners, this investigation constructs HF-PPI between <i>H. sapiens</i> and <i>C. albicans</i>. Our knowledge of human-fungal pathogen protein–protein interactions will be improved by integrating computational and experimental data in order to facilitate the development of efficient fungal infection prevention and treatment protocols.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 5","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143786623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and application of an interbacterial DNA delivery system based on M13 bacteriophage 基于 M13 噬菌体的细菌间 DNA 运送系统的开发与应用

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2025-04-05 DOI: 10.1007/s00203-025-04309-z

Li Qu, Zhou Chi, Bang-Ce Ye

{"title":"Development and application of an interbacterial DNA delivery system based on M13 bacteriophage","authors":"Li Qu, Zhou Chi, Bang-Ce Ye","doi":"10.1007/s00203-025-04309-z","DOIUrl":"10.1007/s00203-025-04309-z","url":null,"abstract":"<div><p>With the rapid development in synthetic biology, bacterial therapy has been widely applied in disease diagnosis and treatment. A key step in regulating the execution of specific instructions by engineered bacteria in vivo involves the transfer of information between bacteria. Currently, small molecule compounds and proteins are commonly used as carriers for inter-bacterial information transfer. However, DNA, as an information carrier, boasts higher information capacity and ease of editing. Based on this, we have developed an engineered bacterial DNA delivery system using M13 bacteriophage to study the cooperative response mechanisms between bacteria. Firstly, we leveraged the ability of engineered bacteria to secrete phagemid DNA into the extracellular environment via bacteriophages and to acquire phagemid DNA through infection by extracellular phagemids, thereby developing a dynamic phagemid replenishment system. This system can maintain the stability of phagemid DNA in environments lacking antibiotic selection pressure, constructing a stable DNA delivery platform for phagemid output. Subsequently, using this engineered bacterial DNA delivery system, we modularized gene circuits and placed them in two different engineered bacteria. Through DNA transfer, we achieved a cooperative response to signals between the two bacteria, laying the groundwork for multi-bacterial joint regulation. Our research not only provides an effective tool for information transfer in engineered bacteria but also offers a novel approach for multi-bacterial therapy.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 5","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deciphering antibiotic resistance, quorum sensing, and biofilm forming genes of Micrococcus luteus from hemodialysis tunneled cuffed catheter tips of renal failure patients 解密肾衰竭患者血液透析隧道袖带导管尖端黄体微球菌的抗生素耐药性、法定量感应和生物膜形成基因

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2025-04-05 DOI: 10.1007/s00203-025-04310-6

Rajsekhar Adhikary, Indrani Sarkar, Dhara Patel, Sishir Gang, Uttam Kumar Nath, Saugata Hazra

{"title":"Deciphering antibiotic resistance, quorum sensing, and biofilm forming genes of Micrococcus luteus from hemodialysis tunneled cuffed catheter tips of renal failure patients","authors":"Rajsekhar Adhikary, Indrani Sarkar, Dhara Patel, Sishir Gang, Uttam Kumar Nath, Saugata Hazra","doi":"10.1007/s00203-025-04310-6","DOIUrl":"10.1007/s00203-025-04310-6","url":null,"abstract":"<div><p>Catheter-related bloodstream infections create a significant challenge in healthcare system, often complicated by antibiotic resistance and biofilm formation of multi-drug resistance and virulent bacterial pathogens. This study focused on biofilm-forming efficiency, and underlying genetic mechanisms in <i>Micrococcus luteus</i> HL_Chru_C<sub>3</sub>, isolated from a hemodialysis catheter tip. The isolate exhibited resistance to multiple antibiotic classes, including beta-lactams and glycopeptides. Biofilm assays revealed that <i>M. luteus</i> HL_Chru_C<sub>3</sub> formed optimum biofilms at high concentration of carbohydrates (500 mM), and pH 5 but there was no significant role of mineral salts. Whole-genome sequencing and bioinformatic analysis using CARD, KAAS, and KEGG databases identified genes associated with antibiotic resistance (<i>ftsI</i>, <i>pbp1a/2</i>, <i>vanY</i>, <i>alr</i>, <i>ddl</i>, <i>murF</i>, <i>mraY</i>, <i>and murG</i>), quorum sensing (genes from the <i>opp</i> family, <i>sec</i>, <i>cylA</i>, <i>ccfA</i>, <i>phnA</i>, <i>phnB</i>, <i>phzC</i>, <i>rpfB</i>, <i>clp</i>, <i>and toxE</i>), and biofilm formation (<i>phnA</i>, <i>phnB</i>, <i>cyaB</i>, <i>vfr</i>, <i>vps</i>, <i>glgC</i>, <i>wecB</i>, <i>wecC</i>, <i>and cysE</i>). The predicted mechanisms of action for these genes, based on homology to other organisms, suggest complex interactions contributing to the observed phenotypes. This study provides an insight into the genetic basis of antibiotic resistance and biofilm formation in <i>M. luteus</i> HL_Chru_C<sub>3</sub> isolated from a hemodialysis catheter, highlighting the need for effective infection control strategies to combat CRBSIs.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 5","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0