Archives of Microbiology最新文献_第10页

Prevalence and molecular characterization of multidrug-resistant coagulase negative staphylococci from urban wastewater in Delhi-NCR, India 印度德里-西北地区城市污水中耐多药凝固酶阴性葡萄球菌的流行情况和分子特征

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-09-10 DOI: 10.1007/s00203-024-04124-y

Sonali Rajput, Sayani Mitra, Aftab Hossain Mondal, Himani Kumari, Kasturi Mukhopadhyay

{"title":"Prevalence and molecular characterization of multidrug-resistant coagulase negative staphylococci from urban wastewater in Delhi-NCR, India","authors":"Sonali Rajput, Sayani Mitra, Aftab Hossain Mondal, Himani Kumari, Kasturi Mukhopadhyay","doi":"10.1007/s00203-024-04124-y","DOIUrl":"10.1007/s00203-024-04124-y","url":null,"abstract":"<div><p>Antimicrobial resistance (AMR) is global health concern escalating rapidly in both clinical settings and environment. The effluent from pharmaceuticals and hospitals may contain diverse antibiotics, exerting selective pressure to develop AMR. To study the aquatic prevalence of drug-resistant staphylococci, sampling was done from river Yamuna (3 sites) and wastewater (7 sites) near pharmaceutical industries in Delhi-NCR, India. 59.25% (224/378) were considered presumptive staphylococci while, methicillin resistance was noted in 25% (56/224) isolates. Further, 23 methicillin-resistant coagulase negative staphylococci (MR-CoNS) of 8 different species were identified via 16S rRNA gene sequencing. Multidrug resistance (MDR) was noted in 60.87% (14/23) isolates. PCR based detection of antibiotic resistance genes revealed the number of isolates containing <i>mecA</i> (7/23), <i>blaZ</i> (6/23), <i>msrA</i> (10/23), <i>aac</i><i>(6′)</i><i>aph</i><i> (2”)</i> (2/23), <i>aph</i><i>(3′)</i>-<i>IIIa</i> (2/23), <i>ant(4′)-Ia</i> (1/23), <i>dfrG</i> (4/23), <i>dfrA</i>(<i>drf</i><i>S1</i>) (3/23), <i>tetK</i> (1/23) and <i>tetM</i> (1/23). The current research highlights the concerning prevalence of MDR-CoNS in aquatic environment in Delhi.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 10","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00203-024-04124-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142196088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of probiotic efficacy of indigenous yeast strain, Saccharomyces cerevisiae Y-89 isolated from a traditional fermented beverage of West Bengal, India having protective effect against DSS-induced colitis in experimental mice 从印度西孟加拉邦一种传统发酵饮料中分离出的本土酵母菌株--酿酒酵母 Y-89 的益生菌功效评估，该菌株对 DSS 诱导的实验性小鼠结肠炎具有保护作用

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-09-10 DOI: 10.1007/s00203-024-04128-8

Moubonny Das, Somasri Dam

{"title":"Evaluation of probiotic efficacy of indigenous yeast strain, Saccharomyces cerevisiae Y-89 isolated from a traditional fermented beverage of West Bengal, India having protective effect against DSS-induced colitis in experimental mice","authors":"Moubonny Das, Somasri Dam","doi":"10.1007/s00203-024-04128-8","DOIUrl":"10.1007/s00203-024-04128-8","url":null,"abstract":"<div><p>Increasing awareness regarding health promotion and disease prevention has driven inclusion of fermented foods and beverages in the daily diet. These are the enormous sources of beneficial microbes, probiotics. This study aims to isolate yeast strains having probiotic potential and effectivity against colitis. Initially, ninety-two yeast strains were isolated from Haria, an ethnic fermented beverage of West Bengal, India. Primary screening was done by their acid (pH 4) and bile salt (0.3%) tolerance ability. Four potent isolates were selected and found effective against <i>Entamoeba histolytica</i>, as this human pathogen is responsible to cause colitis. They were identified as <i>Saccharomyces cerevisiae</i>. They showed luxurious growth even at 37 <sup>o</sup>C, tolerance up to 5% of NaCl, resistance to gastric juice and high bile salt (2.0%) and oro-gastrointestinal transit tolerance. They exhibited good auto-aggregation and co-aggregation ability and strong hydrophobicity. Finally, heat map and principal component analysis revealed that strain Y-89 was the best candidate. It was further characterised and found to have significant protective effects against DSS-induced colitis in experimental mice model. It includes improvement in colon length, body weight and organ indices; reduction in disease activity index; reduction in cholesterol, LDL, SGPT, SGOT, urea and creatinine levels; improvement in HDL, ALP, total protein and albumin levels; decrease in coliform count and restoration of tissue damage. This study demonstrates that the <i>S. cerevisiae</i> strain Y-89 possesses remarkable probiotic traits and can be used as a potential bio-therapeutic candidate for the prevention of colitis.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 10","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142196086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosynthesis and one-step enrichment process of potentially prebiotic cello-oligosaccharides produced by β-glucosidase from Fusarium solani 由茄科镰刀菌中β-葡萄糖苷酶产生的潜在益生元纤维寡糖的生物合成和一步法富集过程。

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-09-09 DOI: 10.1007/s00203-024-04111-3

Manel Boudabbous, Ines Ben Hmad, Mariem Zaidi, Walid Saibi, Lobna Jlaiel, Ali Gargouri

{"title":"Biosynthesis and one-step enrichment process of potentially prebiotic cello-oligosaccharides produced by β-glucosidase from Fusarium solani","authors":"Manel Boudabbous, Ines Ben Hmad, Mariem Zaidi, Walid Saibi, Lobna Jlaiel, Ali Gargouri","doi":"10.1007/s00203-024-04111-3","DOIUrl":"10.1007/s00203-024-04111-3","url":null,"abstract":"<div><p>Cello-oligosaccharides (COS) become a new type of functional oligosaccharides. COS transglycosylation reactions were studied to enhance COS yield production. Seeking the ability of the free form of <i>Fusarium solani</i> β-glucosidase (FBgl1) to synthesize COS under low substrate concentrations, we found out that this biocatalyst initiates this reaction with only 1 g/L of cellobiose, giving rise to the formation of cellotriose. Cellotriose and cellopentaose were detected in biphasic conditions with an immobilized FBgl1 and when increased to 50 g/L of cellobiose as a starter concentration. After the biocatalyst recycling process, the trans-glycosylation yield of COS was maintained after 5 cycles, and the COS concentration was 6.70 ± 0.35 g/L. The crude COS contained 20.15 ± 0.25 g/L glucose, 23.15 ± 0.22 g/L non-reacting substrate cellobiose, 5.25 ± 0.53 g/L, cellotriose and 1.49 ± 0.32 g/L cellopentaose. A bioprocess was developed for cellotriose enrichment, using whole <i>Bacillus velezensis</i> cells as a microbial purification tool. This bacteria consumed glucose, unreacted cellobiose, and cellopentaose while preserving cellotriose in the fermented medium. This study provides an excellent enzyme candidate for industrial COS production and is also the first study on the single-step COS enrichment process.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 10","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142153039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genotypic and phenotypic characterisation of asymptomatic bacteriuria (ABU) isolates displaying bacterial interference against multi-drug resistant uropathogenic E. Coli 无症状菌尿（ABU）分离物的基因型和表型特征，显示细菌对多重耐药尿路致病性大肠杆菌的干扰。

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-09-09 DOI: 10.1007/s00203-024-04114-0

Ciara Kenneally, Craig P. Murphy, Roy D. Sleator, Eamonn P. Culligan

{"title":"Genotypic and phenotypic characterisation of asymptomatic bacteriuria (ABU) isolates displaying bacterial interference against multi-drug resistant uropathogenic E. Coli","authors":"Ciara Kenneally, Craig P. Murphy, Roy D. Sleator, Eamonn P. Culligan","doi":"10.1007/s00203-024-04114-0","DOIUrl":"10.1007/s00203-024-04114-0","url":null,"abstract":"<div><p><i>Escherichia coli</i> can colonise the urogenital tract of individuals without causing symptoms of infection, in a condition referred to as asymptomatic bacteriuria (ABU). ABU isolates can protect the host against symptomatic urinary tract infections (UTIs) by bacterial interference against uropathogenic <i>E. coli</i> (UPEC). The aim of this study was to investigate the genotypic and phenotypic characteristics of five ABU isolates from midstream urine samples of adults. Comparative genomic and phenotypic analysis was conducted including an antibiotic resistance profile, pangenome analysis, and a putative virulence profile. Based on the genome analysis, the isolates consisted of one from phylogroup A, three from phylogroup B2, and one from phylogroup D. Two of the isolates, PUTS 58 and SK-106-1, were noted for their lack of antibiotic resistance and virulence genes compared to the prototypic ABU strain <i>E. coli</i> 83,972. This study provides insights into the genotypic and phenotypic profiles of uncharacterised ABU isolates, and how relevant fitness and virulence traits can impact their potential suitability for therapeutic bacterial interference.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 10","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11381485/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142153041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of cyclotides Mra30 and cycloviolacin O17 derived from Viola dalatensis Gadnep 从 Viola dalatensis Gadnep 中提取的环素 Mra30 和环紫杉素 O17 的特征。

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-09-09 DOI: 10.1007/s00203-024-04122-0

Tien T. Dang, Tam T. T. Tran, Son H. Pham, Tong-Hung Quach, Nhu T.Q. Ngo, Tuan H.N. Nguyen

{"title":"Characterization of cyclotides Mra30 and cycloviolacin O17 derived from Viola dalatensis Gadnep","authors":"Tien T. Dang, Tam T. T. Tran, Son H. Pham, Tong-Hung Quach, Nhu T.Q. Ngo, Tuan H.N. Nguyen","doi":"10.1007/s00203-024-04122-0","DOIUrl":"10.1007/s00203-024-04122-0","url":null,"abstract":"<div><p>Bacteria threaten human and animal health, and standard antibiotics no longer effective. Antibiotic-resistant microorganisms can make infection treatment challenging and perhaps fail. Investigating the attributes of cyclotide, a peptide with promising antibacterial properties that holds great potential in the field of antibiotic research. The structure of these cyclic peptides involves six conserved cysteine residues that form three disulfide bonds, resulting in a cyclic cystine knot (CCK). This feature guarantees their durability when exposed to changes in temperature, chemicals, and enzymatic degradation. The two cyclotides, cycloviolacin O17 and mra30, were obtained from <i>Viola dalatensis</i> Gadnep through a series of techniques including the use of a 50% acetonitrile/49% miliQ water/1% formic acid solution for extraction, ammonium salt precipitation, RP-HPLC purification and sequence identification by LC-MS/MS. These cyclotides exhibit antibacterial effects on specific strains of bacteria like <i>Staphylococcus aureus</i>, <i>Bacillus subtilis</i>, and <i>Pseudomonas aeruginosa</i> at a concentration of 0.2 mg/mL, leading to inhibition zones ranging from 10 to 14 mm. In addition, the disulfide bonds play a crucial role in the antibacterial function of cyclotides. Disrupting the disulfide bonds through ankylation reaction results in the loss of antibacterial properties in the cyclotides (cyO17 and mra30). The minimum inhibitory concentration (MIC) values of mra30 and cyO17 are significantly low, ranging from 0.1 to 0.6 µM. These values are approximately three times lower than the MIC values observed in salt precipitation samples.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 10","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142153040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biofilm-specific determinants of enterococci pathogen 肠球菌病原体的生物膜特异性决定因素。

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-09-09 DOI: 10.1007/s00203-024-04119-9

Rohit Ruhal, Abhijeet Sahu, Tushar Koujalagi, Ankumoni Das, Hema Prasanth, Rashmi Kataria

{"title":"Biofilm-specific determinants of enterococci pathogen","authors":"Rohit Ruhal, Abhijeet Sahu, Tushar Koujalagi, Ankumoni Das, Hema Prasanth, Rashmi Kataria","doi":"10.1007/s00203-024-04119-9","DOIUrl":"10.1007/s00203-024-04119-9","url":null,"abstract":"<div><p>Amongst all <i>Enterococcus spp</i>., <i>E. faecalis</i> and <i>E. faecium</i> are most known notorious pathogen and their biofilm formation has been associated with endocarditis, oral, urinary tract, and wound infections. Biofilm formation involves a pattern of initial adhesion, microcolony formation, and mature biofilms. The initial adhesion and microcolony formation involve numerous surface adhesins e.g. pili Ebp and polysaccharide Epa. The mature biofilms are maintained by eDNA, It’s worth noting that phage-mediated dispersal plays a prominent role. Further, the involvement of peptide pheromones in regulating biofilm maintenance sets it apart from other pathogens and facilitating the horizontal transfer of resistance genes. The role of <i>fsr</i> based regulation by regulating <i>gelE</i> expression is also discussed. Thus, we provide a concise overview of the significant determinants at each stage of <i>Enterococcus</i> spp. biofilm formation. These elements could serve as promising targets for antibiofilm strategies.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 10","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142153038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cold-adapted live attenuated MERS-CoV vaccine strain remains attenuated in mice after multiple passages in Vero cells at 37 °C 冷适配 MERS-CoV 减毒活疫苗株在 37 °C的 Vero 细胞中多次传代后，在小鼠体内仍能保持减毒作用。

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-09-06 DOI: 10.1007/s00203-024-04120-2

Heejeong Seo, Yunyueng Jang

{"title":"Cold-adapted live attenuated MERS-CoV vaccine strain remains attenuated in mice after multiple passages in Vero cells at 37 °C","authors":"Heejeong Seo, Yunyueng Jang","doi":"10.1007/s00203-024-04120-2","DOIUrl":"10.1007/s00203-024-04120-2","url":null,"abstract":"<div><p>Middle East respiratory syndrome coronavirus (MERS-CoV) is a zoonotic disease affecting camels and humans. The live attenuated vaccine represents a candidate human vaccine because it can induce strong immune responses in immunized hosts. The attenuated vaccine strain of the highly pathogenic virus can also be used to produce a cell-based vaccine in the BSL2 GMP facility. In this study, we evaluated the reversion potential of pathogenicity to pathogenic wild-type virus to ensure the safety of the live attenuated vaccine strain. We passaged our previously developed cold-adapted live attenuated MERS-CoV vaccine strain at 22 °C (EMC2012-CA22°C<b>)</b> in Vero cells at 37 °C as often as 15 times to determine the potential of pathogenicity reversion in hDPP4 (human dipeptidyl peptidase 4)-transgenic mice, K18-hDPP4. The serial passage of EMC2012-CA22°C in Vero cells at 37 °C up to 15 times did not result in pathogenicity reversion to wild-type MERS-CoV. In K18-hDPP4 mice infected with this virus, no weight loss or mortality was observed, and no virus was detected in tissues such as the lung, kidney, brain, and nasal turbinate. In addition, mice immunized with this virus produced a robust neutralizing antibody response and were fully protected from lethal challenge with wild-type MERS-CoV. The cold-adapted attenuated MERS-CoV vaccine strain (EMC2012-CA22°C) was not reverted to wild-type pathogenic virus after 15 passages in Vero cells at 37 °C.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 10","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142139142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of low frequency alternating magnetic field for erythritol production in Yarrowia lipolytica 低频交变磁场对脂肪分解酵母生产赤藓糖醇的影响。

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-09-04 DOI: 10.1007/s00203-024-04115-z

Hong Wang, Jiayang Hou, Dongxu Wang, Hu Shi, Luqian Gong, Xuemeng Lv, Jinlong Liu

{"title":"Effect of low frequency alternating magnetic field for erythritol production in Yarrowia lipolytica","authors":"Hong Wang, Jiayang Hou, Dongxu Wang, Hu Shi, Luqian Gong, Xuemeng Lv, Jinlong Liu","doi":"10.1007/s00203-024-04115-z","DOIUrl":"10.1007/s00203-024-04115-z","url":null,"abstract":"<div><p>Numerous works have reported that magnetic fields serve as signals capable of influencing microbial metabolism. However, little is known about the effect of magnetic field on erythritol production by the model microorganism <i>Yarrowia lipolytica</i> (<i>Y. lipolytica</i>). Therefore, we investigated the effect of low-frequency alternating magnetic fields (LF-AMF) with different magnetic field intensities (0–1.5 mT) and different magnetic field treatment times (1–10 days) on the production of erythritol by <i>Y. lipolytica</i> -JZ204. The optimal treatment condition was 0.5 mT for 8 days. As a result, a maximal erythritol yield was achieved 63.74 g/L, the biomass was reached 37 g/L, and the specific erythritol yield per unit of biomass was 1.7227 g/g, which were 60.72%, 32.09%, and 24.85% higher than the control, respectively. We investigated the internal mechanism of magnetic fields impact by using transcriptomics and RT-qPCR technology. This study demonstrated the effectiveness of LF-AMF in enhancing erythritol production by <i>Y. lipolytica</i> JZ-204, providing insights for the application of magnetic field in assisting microbial fermentation and improving the synthesis of beneficial products.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 10","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142124634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of genes associated with the high-temperature fermentation trait in the Saccharomyces cerevisiae natural isolate BCC39850 鉴定与酿酒酵母天然分离物 BCC39850 高温发酵特性相关的基因。

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-09-04 DOI: 10.1007/s00203-024-04117-x

Warasirin Sornlek, Nattida Suwanakitti, Chutima Sonthirod, Sithichoke Tangphatsornruang, Supawadee Ingsriswang, Weerawat Runguphan, Lily Eurwilaichtr, Sutipa Tanapongpipat, Verawat Champreda, Niran Roongsawang, Peter J. Schaap, Vitor A. P. Martins dos Santos

{"title":"Identification of genes associated with the high-temperature fermentation trait in the Saccharomyces cerevisiae natural isolate BCC39850","authors":"Warasirin Sornlek, Nattida Suwanakitti, Chutima Sonthirod, Sithichoke Tangphatsornruang, Supawadee Ingsriswang, Weerawat Runguphan, Lily Eurwilaichtr, Sutipa Tanapongpipat, Verawat Champreda, Niran Roongsawang, Peter J. Schaap, Vitor A. P. Martins dos Santos","doi":"10.1007/s00203-024-04117-x","DOIUrl":"10.1007/s00203-024-04117-x","url":null,"abstract":"<div><p>The fermentative model yeast <i>Saccharomyces cerevisiae</i> has been extensively used to study the genetic basis of stress response and homeostasis. In this study, we performed quantitative trait loci (QTL) analysis of the high-temperature fermentation trait of the progeny from the mating of the <i>S. cerevisiae</i> natural isolate BCC39850 (haploid#17) and the laboratory strain CEN.PK2-1C. A single QTL on chromosome X was identified, encompassing six candidate genes (<i>GEA1</i>, <i>PTK2</i>, <i>NTA1</i>, <i>NPA3</i>, <i>IRT1</i>, and <i>IML1</i>). The functions of these candidates were tested by reverse genetic experiments. Deletion mutants of <i>PTK2</i>, <i>NTA1</i>, and <i>IML1</i> showed growth defects at 42 °C. The <i>PTK2</i> knock-out mutant also showed significantly reduced ethanol production and plasma membrane H<sup>+</sup> ATPase activity and increased sensitivity to acetic acid, ethanol, amphotericin B (AMB), and <i>β</i>-1,3-glucanase treatment. The CRISPR-Cas9 system was used to construct knock-in mutants by replacement of <i>PTK2</i>, <i>NTA1</i>, <i>IML1</i>, and <i>NPA3</i> genes with BCC39850 alleles. The <i>PTK2</i> and <i>NTA1</i> knock-in mutants showed increased growth and ethanol production titers at 42 °C. These findings suggest an important role for the <i>PTK2</i> serine/threonine protein kinase in regulating plasma membrane H<sup>+</sup> ATPase activity and the <i>NTA1</i> N-terminal amidase in protein degradation via the ubiquitin-proteasome system machinery, which affects tolerance to heat stress in <i>S. cerevisiae</i>.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 10","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142124635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tryptophan production by catalysis of a putative tryptophan synthase protein 通过推测的色氨酸合成酶蛋白的催化作用产生色氨酸。

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-09-02 DOI: 10.1007/s00203-024-04123-z

Lulu Cao, Jiaqi Zhang, Jia Chen, Mei Li, Hao Chen, Chongju Wang, Chunjie Gong

{"title":"Tryptophan production by catalysis of a putative tryptophan synthase protein","authors":"Lulu Cao, Jiaqi Zhang, Jia Chen, Mei Li, Hao Chen, Chongju Wang, Chunjie Gong","doi":"10.1007/s00203-024-04123-z","DOIUrl":"10.1007/s00203-024-04123-z","url":null,"abstract":"<div><p>Essential amino acid, tryptophan which intake from food plays a critical role in numerous metabolic functions, exhibiting extensive biological functions and applications. Tryptophan is beneficial for the food sector by enhancing nutritional content and promoting the development of functional foods. A putative gene encoding tryptophan synthase was the first identified in <i>Sphingobacterium soilsilvae</i> Em02, a cellulosic bacterium making it inherently more environmentally friendly. The gene was cloned and expressed in exogenous host <i>Escherichia coli</i>, to elucidate its function. The recombinant tryptophan synthase with a molecular weight 42 KDa was expressed in soluble component. The enzymatic activity to tryptophan synthase in vivo was assessed using indole and L-serine and purified tryptophan synthase. The optimum enzymatic activity for tryptophan synthase was recorded at 50 ºC and pH 7.0, which was improved in the presence of metal ions Mg<sup>2+</sup>, Sr<sup>2+</sup> and Mn<sup>2+</sup>, whereas Cu<sup>2+</sup>, Zn<sup>2+</sup> and Co<sup>2+</sup> proved to be inhibitory. Using site-directed mutagenesis, the consensus pattern HK-S-[GGGSN]-E-S in the tryptophan synthase was demonstrated with K100Q, S202A, G246A, E361A and S385A as the active sites. Tryptophan synthase has been demonstrated to possess the defining characteristics of the β-subunits. The tryptophan synthase may eventually be useful for tryptophan production on a larger scale. Its diverse applications highlight the potential for improving both the quality and health benefits of food products, making it an essential component in advancing food science and technology.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 9","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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