Archives of Microbiology最新文献_第9页

Production and characterization of bacterial cellulose synthesized by Enterobacter chuandaensis strain AEC using Phoenix dactylifera and Musa acuminata 川达肠杆菌 AEC 菌株利用凤凰木和尖叶麝香合成的细菌纤维素的生产和特性分析

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-10-29 DOI: 10.1007/s00203-024-04182-2

Ashraf Sami Hassan AL-Hasabe, Ahmad Faizal Bin Abdull Razis, Nadiya Akmal Binti Baharum, Choo Yee Yu, Nurulfiza Mat Isa

{"title":"Production and characterization of bacterial cellulose synthesized by Enterobacter chuandaensis strain AEC using Phoenix dactylifera and Musa acuminata","authors":"Ashraf Sami Hassan AL-Hasabe, Ahmad Faizal Bin Abdull Razis, Nadiya Akmal Binti Baharum, Choo Yee Yu, Nurulfiza Mat Isa","doi":"10.1007/s00203-024-04182-2","DOIUrl":"10.1007/s00203-024-04182-2","url":null,"abstract":"<div><p>Bacterial cellulose (BC) is a biopolymer synthesized extracellularly by certain bacteria through the polymerization of glucose monomers. This study aimed to produce BC using <i>Enterobacter chuandaensis</i> with fruit extracts from <i>Phoenix dactylifera</i> (D) and <i>Musa acuminata</i> (M) as carbon sources. Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy (ATR-FTIR) showed characteristic cellulose vibrations, while X-ray diffraction (XRD) identified distinct peaks at 15.34°, 19.98°, 22.58°, and 34.6°, confirming the cellulose structure. Whole-genome sequencing of <i>E. chuandaensis</i> identified key genes involved in BC production. The BC produced then exhibited a molecular weight of 1,857,804 g/mol, with yields of 2.8 g/L and 2.5 g/L for treatments D and M, respectively. The crystallinity index of the purified BC was 74.1, and <sup>13</sup>C NMR analysis confirmed the dominant cellulose Iα crystalline form. The BC showed high biocompatibility in cytotoxicity assays, with cell viability between 92% and 100%, indicating its potential for use in biomedical applications. This investigation represents the first report of BC production by <i>E. chuandaensis</i>, which promises a new avenue for sustainable and efficient BC synthesis using fruit extracts as carbon sources.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 11","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142524338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenotypic heterogeneity in bacteria: the rise of antibiotic persistence, clinical implications, and therapeutic opportunities 细菌的表型异质性：抗生素持久性的上升、临床影响和治疗机会。

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-10-26 DOI: 10.1007/s00203-024-04173-3

Srimayee Pani, Saswat S. Mohapatra

{"title":"Phenotypic heterogeneity in bacteria: the rise of antibiotic persistence, clinical implications, and therapeutic opportunities","authors":"Srimayee Pani, Saswat S. Mohapatra","doi":"10.1007/s00203-024-04173-3","DOIUrl":"10.1007/s00203-024-04173-3","url":null,"abstract":"<div><p>The rising incidence of antimicrobial resistance (AMR) and the diminishing antibiotics discovery pipeline have created an unprecedented scenario where minor infections could become untreatable. AMR phenomenon is genetically encoded, and various genetic determinants have been implicated in their emergence and spread. Nevertheless, several non-genetic phenomena are also involved in antibiotic treatment failure which requires a systematic investigation. It has been observed that in an isogenic population of bacteria, not all cells behave or respond the same way to an antibiotic, because of the inherent heterogeneity among them. This heterogeneity is not always heritable but rather phenotypic. Three distinct types of phenotypic heterogeneity, namely tolerance, persistence, and heteroresistance have been observed in bacteria having significant clinical implications influencing the treatment outcome. While tolerance is when a population can survive high doses of antibiotics without changing the minimum inhibitory concentration (MIC) of the drug, persistence occurs in a subpopulation of bacteria that can survive exposure to high antibiotic doses. In contrast, when a subpopulation shows a very high MIC in comparison to the rest of the population, the phenomenon is called heteroresistance. In this article, we have highlighted bacterial persistence with a focus on their emergence and the underlying molecular mechanisms. Moreover, we have tried to associate the genome-wide methylation status with that of the heterogeneity at a single-cell level that may explain the role of epigenetic mechanisms in the development of persistence.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 11","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142493651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacteriophage entrapment strategies for the treatment of chronic wound infections: a comprehensive review 治疗慢性伤口感染的噬菌体诱捕策略：全面综述。

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-10-23 DOI: 10.1007/s00203-024-04168-0

Nivedya Mohan, Kiran Bosco, Anmiya Peter, K. Abhitha, Sarita G. Bhat

{"title":"Bacteriophage entrapment strategies for the treatment of chronic wound infections: a comprehensive review","authors":"Nivedya Mohan, Kiran Bosco, Anmiya Peter, K. Abhitha, Sarita G. Bhat","doi":"10.1007/s00203-024-04168-0","DOIUrl":"10.1007/s00203-024-04168-0","url":null,"abstract":"<div><p>The growing threat of antimicrobial resistance has made the quest for antibiotic alternatives or synergists one of the most pressing priorities of the 21st century. The emergence of multidrug-resistance in most of the common wound pathogens has amplified the risk of antibiotic-resistant wound infections. Bacteriophages, with their self-replicating ability and targeted specificity, can act as suitable antibiotic alternatives. Nevertheless, targeted delivery of phages to infection sites remains a crucial issue, specifically in the case of topical infections. Hence, different phage delivery systems have been studied in recent years. However, there have been no recent reviews of phage delivery systems focusing exclusively on phage application on wounds. This review provides a compendium of all the major delivery systems that have been used to deliver phages to wound infection sites. Special focus has also been awarded to phage-embedded hydrogels with a discussion on the different aspects to be considered during their preparation.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 11","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142493650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stability characterizations of feed-based bivalent vaccine containing inactivated Streptococcus agalactiae and Aeromonas hydrophila against streptococcosis and Aeromonas infections in red hybrid tilapia (Oreochromis sp.) 含有灭活无乳链球菌和嗜水气单胞菌的饲料型二价疫苗对红色杂交罗非鱼（Oreochromis sp.）链球菌病和嗜水气单胞菌感染的稳定性特征。

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-10-23 DOI: 10.1007/s00203-024-04166-2

Nur Shidaa Mohd Ali, Mohamad Syazwan Ngalimat, Mohd Zamri Saad, Mohammad Noor Amal Azmai, Annas Salleh, Zarirah Zulperi, Ina Salwany Md Yasin

{"title":"Stability characterizations of feed-based bivalent vaccine containing inactivated Streptococcus agalactiae and Aeromonas hydrophila against streptococcosis and Aeromonas infections in red hybrid tilapia (Oreochromis sp.)","authors":"Nur Shidaa Mohd Ali, Mohamad Syazwan Ngalimat, Mohd Zamri Saad, Mohammad Noor Amal Azmai, Annas Salleh, Zarirah Zulperi, Ina Salwany Md Yasin","doi":"10.1007/s00203-024-04166-2","DOIUrl":"10.1007/s00203-024-04166-2","url":null,"abstract":"<div><p>Feed-based bivalent vaccine (FBBV) containing killed whole organism (KWO) of <i>Streptococcus agalactiae</i> and <i>Aeromonas hydrophila</i> with 10% palm oil was previously proved to improve red hybrid tilapia’s (<i>Oreochromis</i> sp.) immunity against streptococcosis and <i>Aeromonas</i> infections. This study characterized the FBBV’s stability following the preparatory process and storage. The FBBV was prepared, and the KWO’s stability was determined microscopically and molecularly. The efficacy of FBBV stored at room temperature (25 ± 2 °C) for 0, 30 and 60 days was investigated in red hybrid tilapia. The results indicated the addition of palm oil was not affecting the KWO’s structure and helping in the FBBV’s pelletization. In 1 g of FBBV contained 1.5 × 10<sup>9</sup> CFU/g of <i>S. agalactiae</i> and 4.9 × 10<sup>9</sup> CFU/g of <i>A. hydrophila</i>, respectively, even after 60 days of storage at room temperature. The KWO’s structure in FBBV was not affected following in vitro acidic tolerance analysis, as noted from light and electron microscopies. The FBBV’s carbohydrate, energy, moisture, total protein and total ash contents remained stable at 95% after 60 days of storage at room temperature, while the KWO’s concentration was slightly reduced to 83.3% for <i>S. agalactiae</i> (1.25 × 10<sup>9</sup> CFU/g) and 80.6% for <i>A. hydrophila</i> (3.85 × 10<sup>9</sup> CFU/g), respectively. Fish vaccinated with FBBV that was stored for 0, 30 and 60 days did not show any significant differences (<i>p</i> ≥ 0.05) in the relative percent survival when challenged with pathogenic <i>Streptococcus</i> spp. and <i>Aeromonas</i> spp. These findings suggested that the FBBV is a stable vaccine, which underscores its potential application as aquatic vaccines in aquaculture.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 11","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142493652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibacterial effects and mechanisms of quercetin-β-cyclodextrin complex mediated photodynamic on Escherichia coli O157:H7 槲皮素-β-环糊精复合物介导的光动力对大肠杆菌 O157:H7 的抗菌作用及其机制

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-10-23 DOI: 10.1007/s00203-024-04175-1

Tao Liu, Yuzhang Zhu, Jiahui Wang, Xiangyu Hong, Mi Liu, Chaonan Kong, Rui Zhou, Xianke Li, Lifang Yang

{"title":"Antibacterial effects and mechanisms of quercetin-β-cyclodextrin complex mediated photodynamic on Escherichia coli O157:H7","authors":"Tao Liu, Yuzhang Zhu, Jiahui Wang, Xiangyu Hong, Mi Liu, Chaonan Kong, Rui Zhou, Xianke Li, Lifang Yang","doi":"10.1007/s00203-024-04175-1","DOIUrl":"10.1007/s00203-024-04175-1","url":null,"abstract":"<div><p>Quercetin is a natural flavonoid with antioxidant, anti-inflammatory, and antibacterial properties. This work aimed to formulate quercetin-cyclodextrin microcapsules (QT-β-CD) while examining their photodynamic antibacterial effects and underlying mechanisms in detail. Characterization of the QT-β-CD was conducted using Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and thermogravimetric analysis (TGA). The bacteriostatic effects of UV-A irradiation on <i>Escherichia coli</i> O157:H7 (<i>E. coli</i> O157:H7) were investigated. The photodynamic impact of QT-β-CD was assessed by analyzing hydrogen peroxide (H₂O₂) production. The antimicrobial activity was further elucidated through examinations of cell membrane integrity, protein damage, changes in cellular motility, biofilm formation, and extracellular polysaccharide reduction. The effect of QT-β-CD on <i>LuxS</i> and <i>motA</i> gene expression in <i>E. coli</i> O157:H7 was investigated by RT-qPCR. The findings demonstrated that QT-β-CD exhibited potent photodynamic properties and functioned as an efficient photosensitizer, causing substantial damage to <i>E. coli</i> O157:H7 cells. These results underscore the potential of quercetin as an antimicrobial agent for food preservation.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 11","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142493649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endophytic Streptomyces: an underexplored source with potential for novel natural drug discovery and development 内生链霉菌：一种尚未充分开发的来源，具有发现和开发新型天然药物的潜力

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-10-22 DOI: 10.1007/s00203-024-04169-z

Periyasamy Sivalingam, Maheswaran Easwaran, Dhanraj Ganapathy, S. Farook Basha, John Poté

{"title":"Endophytic Streptomyces: an underexplored source with potential for novel natural drug discovery and development","authors":"Periyasamy Sivalingam, Maheswaran Easwaran, Dhanraj Ganapathy, S. Farook Basha, John Poté","doi":"10.1007/s00203-024-04169-z","DOIUrl":"10.1007/s00203-024-04169-z","url":null,"abstract":"<div><p><i>Streptomyces</i> has long been considered as key sources for natural compounds discovery in medicine and agriculture. These compounds have been demonstrated to possess different biological activities, including antibiotic, antifungal, anticancer, and antiviral effects. As a result, new pharmaceuticals and antibiotics have been developed. Nevertheless, there have been only a few novel discoveries of bioactive compounds in the past decades from <i>Streptomyces</i> in natural habitats. There is, therefore, now a renewed search for new <i>Streptomyces</i> species having the potential to produce many compounds from one strain in lesser explored natural habitats that may be helpful in fighting diseases. Consequently, modern genome mining approaches are imperative for discovering structurally novel natural compounds with therapeutic applications from untapped sources. In light of these facts, endophytic <i>Streptomyces</i> from plants may offer new avenues for the discovery of bioactive compounds with distinctive chemical properties and activities. In the present review, we present the progress made in isolating natural compounds from endophytic <i>Streptomyces</i> originating from plants which have remarkable antimicrobial, cytotoxic, and antifungal properties. A different of distinct structural classes of compounds were reported from endophytic <i>Streptomyces</i>, such as indolosequiterpene, macrolides, flavones, peptides, naphthoquinones, and terpenoids. Further, we discussed modern genomics progress in finding biosynthetic gene clusters (BGCs) encoding compounds. Overall, this review might provide valuable insights into the potential for novel drug discovery from untapped endophytic <i>Streptomyces</i> in the future.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 11","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142453121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Omic-driven strategies to unveil microbiome potential for biodegradation of plastics: a review 揭示微生物组生物降解塑料潜力的 Omic 驱动战略：综述

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-10-21 DOI: 10.1007/s00203-024-04165-3

Shafana Farveen Mohamed, Rajnish Narayanan

{"title":"Omic-driven strategies to unveil microbiome potential for biodegradation of plastics: a review","authors":"Shafana Farveen Mohamed, Rajnish Narayanan","doi":"10.1007/s00203-024-04165-3","DOIUrl":"10.1007/s00203-024-04165-3","url":null,"abstract":"<div><p>Plastic waste accumulation has lately been identified as the leading and pervasive environmental concern, harming all living beings, natural habitats, and the global market. Given this issue, developing ecologically friendly solutions, such as biodegradation instead of standard disposal, is critical. To effectively address and develop better strategies, it is critical to understand the inter-relationship between microorganisms and plastic, the role of genes and enzymes involved in this process. However, the complex nature of microbial communities and the diverse mechanisms involved in plastic biodegradation have hindered the development of efficient plastic waste degradation strategies. Omics-driven approaches, encompassing genomics, transcriptomics and proteomics have revolutionized our understanding of microbial ecology and biotechnology. Therefore, this review explores the application of omics technologies in plastic degradation studies and discusses the key findings, challenges, and future prospects of omics-based approaches in identifying novel plastic-degrading microorganisms, enzymes, and metabolic pathways. The integration of omics technologies with advanced molecular technologies such as the recombinant DNA technology and synthetic biology would guide in the optimization of microbial consortia and engineering the microbial systems for enhanced plastic biodegradation under various environmental conditions.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 11","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142452949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and identification of epiphytic Pichia kudriavzevii from loquat peels and investigation of its fermentation characteristics for liquor production 从枇杷皮中分离和鉴定附生的 Pichia kudriavzevii，并研究其用于白酒生产的发酵特性

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-10-19 DOI: 10.1007/s00203-024-04162-6

Qingfang Xu, Wei Huang, Yanqin Li, Jian Cai, Xiu Gao, Xu Bai, Weiliang Liu, Lifang Zhang, Ling Zhu

{"title":"Isolation and identification of epiphytic Pichia kudriavzevii from loquat peels and investigation of its fermentation characteristics for liquor production","authors":"Qingfang Xu, Wei Huang, Yanqin Li, Jian Cai, Xiu Gao, Xu Bai, Weiliang Liu, Lifang Zhang, Ling Zhu","doi":"10.1007/s00203-024-04162-6","DOIUrl":"10.1007/s00203-024-04162-6","url":null,"abstract":"<div><p>During the process of fruit wine production, yeast plays a crucial role in influencing the taste, flavor, and overall quality of the wine. This study aims to enhance the flavor and quality of <i>loquat</i> wine by isolating strains of <i>Pichia kudriavzevii</i> (<i>P. kudriavzevii</i>) with desirable winemaking characteristics from <i>loquat</i> fruit fermentation broth. A total of 12 strains of <i>P. kudriavzevii</i> were isolated and subjected to morphological and molecular biological identification. Their fermentation performance, ethanol production, ester production, hydrogen sulfide production, killer activity, and tolerance were evaluated. The results revealed that strains Q-2, Q-9, Q-10, Q-12, Q-20, and Q-42 exhibited robust growth and strong tolerance under conditions of 40 °C temperature, 12% ethanol concentration, 350 g/L glucose concentration, and pH 2.8. Strain Q-42 demonstrated the strongest gas production capacity, killer activity, and good ester and ethanol production. As a highly active fermentation strain with excellent wine making characteristics, <i>P. kudriavzevii</i> Q-42 provides a valuable yeast resource for the industrial production of <i>loquat</i> wine and offers technical support for improving the overall quality of <i>loquat</i> wine.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 11","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00203-024-04162-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142451018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessing the effects of NapA gene overexpression on denitrification and denitrogenation in magnetospirillum gryphiswaldense MSR-1 评估NapA基因过表达对磁螺旋体MSR-1中反硝化和反氮化的影响

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-10-19 DOI: 10.1007/s00203-024-04158-2

Jiawei Hu, Meijing Liu, Lu Li, Jinjing Hu, Cong Wang

{"title":"Assessing the effects of NapA gene overexpression on denitrification and denitrogenation in magnetospirillum gryphiswaldense MSR-1","authors":"Jiawei Hu, Meijing Liu, Lu Li, Jinjing Hu, Cong Wang","doi":"10.1007/s00203-024-04158-2","DOIUrl":"10.1007/s00203-024-04158-2","url":null,"abstract":"<div><p>Previous research on <i>Magnetospirillum gryphiswaldense</i> MSR-1 found that MSR-1 has a good denitrification nitrogen removal ability and specific application prospects in the sewage biological nitrogen removal field. Therefore, this study selected the essential denitrification gene NapA in MSR-1-wt for overexpression, and the overexpressed MSR-1-NapA was successfully constructed. Q-PCR amplification experiment and AGAR gel electrophoresis experiment proved that the relative transcription level of the <i>NapA</i> gene was increased by more than four times, and the denitrification ability of MSR-1-wt and MSR-1-NapA was further determined by enzyme activity experiment, denitrification experiment, and flow cytometry. The results showed that overexpression of the <i>NapA</i> gene increased nitrate reductase activity in MSR-1-NapA by more than four times. In the solution with a nitrate concentration of 118.33 ± 3.23 mgN/L, the denitrification efficiency of MSR-1-NapA was superior to that of MSR-1-wt, significantly enhancing both the denitrification and nitrogen removal capacities of MSR-1. This indicates its greater potential for biological nitrogen removal in wastewater treatment.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 11","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142451019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: A rho-type GTPase activating protein affects the growth and development of Cordyceps cicadae 更正为一种Rho型GTP酶活化蛋白影响冬虫夏草的生长发育

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-10-18 DOI: 10.1007/s00203-024-04160-8

Xueqian Li, Yu Zou, Neeraj Shrivastava, Jiandong Bao, Fu-Cheng Lin, Hongkai Wang

引用次数: 0