Archives of Microbiology最新文献

{"title":"Effects of bcsE, luxS and rfaG genes on the physiology and pathogenicity of Salmonella Typhimurium","authors":"Semra Soydam,&nbsp;Elif Gamze Has,&nbsp;Tuba Nur Sürkaç,&nbsp;Mustafa Akçelik,&nbsp;Nefise Akçelik","doi":"10.1007/s00203-025-04248-9","DOIUrl":"10.1007/s00203-025-04248-9","url":null,"abstract":"<div><p>In this study, <i>rfaG</i>, <i>luxS</i>, and <i>bcsE</i> gene mutants of <i>S.</i> Typhimurium ATCC 14028 wild-type strain were generated, and their effects on bacterial physiology and pathogenicity were investigated. Biofilm production capacity and mobility of mutant strains were found to be significantly reduced. Immune response stimulation of 6–8 week-old BALB/c mice infected with wild-type and mutants was evaluated on days 0 and 21 for IL-6, IFN-γ, and IgG, and then systemic infection in mice was determined by the amount of <i>Salmonella</i> recovered from the cecum, spleen, and liver organs. The specific IgG antibody level and the expression level of the cytokines IFN-γ and IL-6 were found to be significantly reduced in mice inoculated with mutant strains compared to the control group. The highest level of colonization in the organs was found in the cecum, and the lowest level of colonization was detected in the liver. In the wild-type-infected group, splenomegaly and staining of the liver were detected. In the groups of mice infected with <i>bcsE</i> and <i>luxS</i> mutants, these symptoms were at a low level. In the groups infected with the <i>rfaG</i> mutant, no significant lesions were detected in the organs.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 3","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143430807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity of secondary metabolites from marine Streptomyces with potential anti-tubercular activity: a review","authors":"Salina Patel,&nbsp;Lincoln Naik,&nbsp;Ankita Rai,&nbsp;Krishna Palit,&nbsp;Ashish Kumar,&nbsp;Mousumi Das,&nbsp;Dev Kiran Nayak,&nbsp;Pramathesh Kumar Dandsena,&nbsp;Amit Mishra,&nbsp;Ramandeep Singh,&nbsp;Rohan Dhiman,&nbsp;Surajit Das","doi":"10.1007/s00203-024-04233-8","DOIUrl":"10.1007/s00203-024-04233-8","url":null,"abstract":"<div><p>The bacterial genus <i>Streptomyces</i> is known for the prolific production of secondary metabolites, which exhibit remarkable structural diversity and potent biological activities. Tuberculosis (TB) remains a formidable global health challenge exacerbated by the emergence of antimicrobial resistance (AMR), necessitating the discovery of novel therapeutic agents. The untapped potential of marine <i>Streptomyces</i>-derived secondary metabolites offers a promising avenue for screening anti-tubercular (anti-TB) compounds with unique chemical structures and potential bioactive properties. The review emphasizes the diverse marine habitats and <i>Streptomyces</i> with novel anti-TB bioactive metabolites. It discusses fermentation and bioprocessing strategies for screening anti-TB drugs. This review also covers the chemical diversity, potency, mechanism of action, and structures of about seventy anti-TB compounds discovered from marine <i>Streptomyces</i>. These compounds span various chemical classes, including quinones, macrolactams, macrolides, phenols, esters, anthracyclines, peptides, glycosides, alkaloids, piperidones, thiolopyrrolones, nucleosides, terpenes, flavonoids, polyketides, and actinomycins. It emphasizes the need to explore marine ecosystems to discover more novel anti-TB natural products.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 3","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143430805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipopeptide iturin C3 from endophytic Bacillus sp. effectively inhibits biofilm formation and prevents the adhesion of topical and food-borne pathogens in vitro and on biomedical devices","authors":"Rajsekhar Adhikary,&nbsp;Pulak Kumar Maiti,&nbsp;Narendranath Ghosh,&nbsp;Biplab Rajbanshi,&nbsp;Mahendra Nath Roy,&nbsp;Sukhendu Mandal,&nbsp;Vivekananda Mandal","doi":"10.1007/s00203-025-04253-y","DOIUrl":"10.1007/s00203-025-04253-y","url":null,"abstract":"<div><p>Iturin, a structurally cyclic heptapeptides with a number of homologous derivatives has broad-spectrum antimicrobial and antibiofilm properties. The present study elucidates the structure and antimicrobial efficacy of iturin C₃ biosurfactant (BS) produced by the endophytic bacterium <i>Bacillus</i> sp. LLB-04. <i>Bacillus</i> sp. LLB-04 was isolated from the leaves of hemiparasite <i>Scurrula parasitica</i> L. during the winter season. The biosurfactant was produced in a static batch culture of 120 h using Nutrient Broth (NB) medium and was extracted by a series of solvent systems. The BS was purified by solvent fractionation and preparative High-Performance Liquid Chromatography (HPLC) method, and then it was structurally characterized through various analytical methods. Its antimicrobial and antibiofilm activities were tested against 0, to 18 h old topical and food-borne pathogens. Furthermore, the cellular aggregation and bacterial cell adhesion on polystyrene and urethral catheters were checked at the Biofilm inhibitory concentration (BIC). The cell line toxicity of BS (0–1.568 mg/ml) was tested against the human embryonic lung tissue L-132 and human alveolar epithelial cancer cell line, and the in silico mode of action was studied using standard methods. From the spectroscopic studies of 96 h culture harvested BS revealed that <i>Bacillus</i> sp. LLB-04 (GenBank Accession No.: MF037706) produced the BS as iturin C₃. The BS had broad-spectrum antimicrobial with minimum inhibitory concentration (MIC) values ranging from 0.1 to 1.6 mg/ml and an average biofilm inhibition concentration (BIC) of 0.8–1.6 mg/ml in 18 h old cells where biofilm formation was inhibited up to 46.4 times at 1.6 mg/ml concentration. It could also destabilize 40–48 h old preformed biofilm and had a synergistic response with streptomycin (<i>Bacillus subtilis</i> MTCC 411, <i>Escherichia coli</i> MTCC 730), ciprofloxacin (<i>B. subtilis</i> MTCC 411, <i>E. coli</i> MTCC 730), and vancomycin (<i>Staphylococcus epidermidis</i> MTCC 3086, <i>B. subtilis</i> MTCC 411). It had antiproliferative activity (0.1–0.8 mg/ml) on cancer cell lines. In-silico protein–ligand interactions predicted that it could interact with different membrane proteins of topical and food-borne pathogens. Thus, the study revealed for the first time that the endophytic <i>Bacillus</i> sp. could be exploited for large-scale production of iturin C₃ that could be used in combating biofilm formation and cellular adhesion of topical and food-borne pathogens.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 3","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143423379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional and structural insights into α-L-Rhamnosidase: cloning, characterization, and decoding evolutionary constraints through structural motif","authors":"Yupeng Liang,&nbsp;Yalan Zhao,&nbsp;Zhongwei Yin,&nbsp;Xin Zeng,&nbsp;Xiulin Han,&nbsp;Mengliang Wen","doi":"10.1007/s00203-025-04259-6","DOIUrl":"10.1007/s00203-025-04259-6","url":null,"abstract":"<div><p><i>α</i>-<span>L</span>-rhamnosidase [E.C. 3.2.1.40] is important in various industrial and biotechnological applications. However, limited knowledge of the structural features of its active site residues and their local geometric arrangements during substrate interaction hinders further application development. In this study, we examined functionally characterized microbial <i>α</i>-<span>L</span>-rhamnosidases. Despite considerable differences in their global structures, the local structures of the substrate-binding sites and key residues were highly conserved. Using the local structural motif, we characterized <i>α</i>-<span>L</span>-rhamnosidase genes from metagenomic samples of traditional fermentation starters. To comprehensively understand the distribution of <i>α</i>-<span>L</span>-rhamnosidases with this motif in the AlphaFold database, we screened 26,858 <i>α</i>-<span>L</span>-rhamnosidase structures. Our findings showed that only 5678 out of 26,858 structures contain the specific conserved motifs, emphasizing their potential significance in mining enzyme function. Moreover, the analysis of structural diversity among representative enzymes demonstrated variation in the number and types of domains within this enzyme family. Further investigation of representative <i>α</i>-L-rhamnosidase sequences with this structural motif confirmed the evolutionary constraints of 15 key residues, indicating strong selective pressures to maintain these elements essential for enzyme functionality. These residues were consistently present across ancestral sequences, underscoring their importance throughout the enzyme’s evolutionary history. This study suggests that structure-guided approaches are valuable for discovering functional enzymes. Identifying conserved motif across diverse microbial taxa not only aids in predicting enzyme functionality but also offers opportunities for enzyme engineering and biotechnological applications.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 3","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143423107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Frontiers in superbug management: innovating approaches to combat antimicrobial resistance","authors":"Priyanka Chambial,&nbsp;Neelam Thakur,&nbsp;Prudhvi Lal Bhukya,&nbsp;Anbazhagan Subbaiyan,&nbsp;Umesh Kumar","doi":"10.1007/s00203-025-04262-x","DOIUrl":"10.1007/s00203-025-04262-x","url":null,"abstract":"<div><p>Anti-microbial resistance (AMR) is a global health issue causing significant mortality and economic burden. Pharmaceutical companies’ discontinuation of research hinders new agents, while MDR pathogens or “superbugs” worsen the problem. Superbugs pose a threat to common infections and medical procedures, exacerbated by limited antibiotic development and rapid antibiotic resistance. The rising tide of antimicrobial resistance threatens to undermine progress in controlling infectious diseases. This review examines the global proliferation of AMR, its underlying mechanisms, and contributing factors. The study explores various methodologies, emphasizing the significance of precise and timely identification of resistant strains. We discuss recent advancements in CRISPR/Cas9, nanoparticle technology, light-based techniques, and AI-powered antibiogram analysis for combating AMR. Traditional methods often fail to effectively combat multidrug-resistant bacteria, as CRISPR-Cas9 technology offers a more effective approach by cutting specific DNA sequences, precision targeting and genome editing. AI-based smartphone applications for antibiogram analysis in resource-limited settings face challenges like internet connectivity, device compatibility, data quality, energy consumption, and algorithmic limitations. Additionally, light-based antimicrobial techniques are increasingly being used to effectively kill antibiotic-resistant microbial species and treat localized infections. This review provides an in-depth overview of AMR covering epidemiology, evolution, mechanisms, infection prevention, control measures, antibiotic access, stewardship, surveillance, challenges and emerging non-antibiotic therapeutic approaches.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 3","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143423288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Standardizing biofilm quantification: harmonizing crystal violet absorbance measurements through extinction coefficient ratio adjustment","authors":"Teena George,&nbsp;Visnuvinayagam Sivam,&nbsp;Murugadas Vaiyapuri,&nbsp;R. Anandan,&nbsp;Gopalan Krishnan Sivaraman,&nbsp;Toms C. Joseph","doi":"10.1007/s00203-025-04251-0","DOIUrl":"10.1007/s00203-025-04251-0","url":null,"abstract":"<div><p>Precise quantification of biofilm is critical as the formation and persistence of biofilm have significant implications in the environmental, therapeutic and industrial contexts. The microtiter plate assay using crystal violet with 33% glacial acetic acid or 94–100% ethanol as the resolubilising agent is widely used for the categorisation of biofilms into weak, moderate and strong categories. But, the use of varying wavelengths for the measurement of biofilm resulted in discrepancies in categorisation across the studies due to the difference in the extinction coefficient of CV. This study emphasises the importance of measuring the biofilm at the absorbance maximum (λ_max) of resolubilized CV, identified as 585 nm for 33% acetic acid and 580 nm for 94–100% ethanol. To address the challenge of harmonizing the results across studies, a method was developed to adjust the biofilm categorisation threshold based on the extinction coefficient ratios of CV at different wavelengths enabling consistent classification regardless of the wavelength used. Validation with <i>E. coli</i> and <i>S. aureus</i> demonstrated that the adjusted thresholds produced results similar to that obtained with the λ_max. This standardised approach not only enables the researchers to obtain accurate and consistent results in the future studies, but also facilitates the comparison of previously published data on biofilm research, which is essential for the exploration of newer therapeutic strategies against biofilm related infections.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 3","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143404182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of heavy metal tolerance and genomics in an indigenous Kurthia strain from Kulik River reveals multi-metal resistance and dominance of selection pressure on codon usage patterns","authors":"Vivek Roy,&nbsp;Monalisha Sarkar Pal,&nbsp;Ayon Pal","doi":"10.1007/s00203-025-04255-w","DOIUrl":"10.1007/s00203-025-04255-w","url":null,"abstract":"<div><p>Heavy metal(loid) contamination poses significant risks to biological entities and the ecosystem. Many metal(loid)-resistant bacteria have been isolated from different environmental sites, but still no work has described multi-metal resistant <i>Kurthia</i> sp. In this study, an indigenous <i>Kurthia</i> strain isolated from the surface water of River Kulik was studied to determine its level of tolerance to various metal(loid)s. This study aimed to isolate, characterize and determine the growth kinetics and efficiency of <i>Kurthia gibsonii</i> strain M6 to remove and bioaccumulate As(V), Ni and Pb in vitro. This study also aimed to sequence the whole genome of the bacterium, identify metal resistance genes and analyze the codon usage patterns and factors that affect the codon usage bias of these genes. The bacterium showed elevated resistance to As(V), Pb, Ni and Zn. Under metal(loid) stressed conditions, live cells of <i>Kurthia</i> strain M6 bioaccumulated 212.74, 91.51 and 40.38 mg g⁻¹ of As(V), Pb and Ni, respectively. The removal efficiency was 97%, 69.15% and 25.88% for Pb, Ni and As(V), respectively. Genome analysis revealed the existence of different genes conferring heavy metal resistance. A comprehensive analysis of codon usage patterns for metal resistance genes depicted the predominance of selection pressure as a prime force influencing codon usage patterns. This is the first time a multi-metal resistant <i>K. gibsonii</i> strain has been systematically studied regarding its heavy metal resistance biology. These findings will provide insights into the metal resistance mechanisms of the genus <i>Kurthia</i> and assist in devising new strategies for the bioremediation of metal-polluted environments.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 3","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143396478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanisms, therapeutic strategies, and emerging therapeutic alternatives for carbapenem resistance in Gram-negative bacteria","authors":"Fatima Mourabiti,&nbsp;Fatimazahra Jouga,&nbsp;Souraya Sakoui,&nbsp;Otmane El Hosayny,&nbsp;Yassine Zouheir,&nbsp;Abdelaziz Soukri,&nbsp;Bouchra El Khalfi","doi":"10.1007/s00203-025-04252-z","DOIUrl":"10.1007/s00203-025-04252-z","url":null,"abstract":"<div><p>Carbapenem-resistant Gram-negative bacteria (CR-GNB) have experienced an alarming surge in prevalence in recent years, escalating into a critical global healthcare crisis. As carbapenems represent the last line of defense against such pathogens, infections caused by CR-GNB have become increasingly challenging to treat, given the restricted therapeutic options and heightened mortality risks. The discovery and development of alternative therapeutic strategies that present novel avenues against multi-drug-resistant organisms are gaining increased attention, presenting a pressing need for innovative solutions. Our comprehensive review delves into the multifaceted landscape of carbapenem resistance in Gram-negative bacteria in response to this urgent challenge. The scope of this review aims to provide an up-to-date and in-depth exploration regarding the mode of action of carbapenem and the resisting mechanisms of carbapenem in GNB. Additionally, it discusses the state of the art of some clinical therapies for the treatment of infections caused by CR-GNB. Moreover, it describes several combinational and alternative therapies to combat CR-GNB, including the computational approach of “molecular docking”. In light of the conclusions of this review, we call for the implementation of these strategies to develop comprehensive approaches to mitigate carbapenem resistance in Gram-negative bacteria.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 3","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143404234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration of piperazine-citral sulfonyl derivatives: antibacterial and in-silico studies against methicillin-resistant Staphylococcus aureus","authors":"K. Nirusha,&nbsp;H. S. Nagendra Prasad,&nbsp;T. N. Lohith,&nbsp;P. Saravanan,&nbsp;L. Mallesha,&nbsp;A. P. Anand","doi":"10.1007/s00203-025-04260-z","DOIUrl":"10.1007/s00203-025-04260-z","url":null,"abstract":"<div><p>This study involved the synthesis and characterization of piperazine-citral sulfonyl derivatives <b>5(a-e)</b> using a variety of spectrum methods, including fourier transform infrared spectroscopy (FT-IR), proton nuclear magnetic resonance (¹H NMR), carbon-nuclear magnetic resonance (¹³C NMR), and liquid chromatography mass spectroscopy (LC–MS). To obtain the energy and other quantum chemical computations of all the piperazine-citral sulfonyl derivatives, the following methods were evaluated: density functional theory (DFT); blood brain barrier (BBB); absorption, distribution, metabolism, and excretion (ADME); and prediction of activity spectra of computational screening (PASS) for their potential approaches for biological applications. The synthesized compounds were examined for drug-likeness, total surface area, polar surface area, H-acceptor and H-donor parameters, clogP and clogS, and other physicochemical features. The significant redesign of the piperazine core with the sulfonyl moiety encourages the search for novel antibacterial candidates among the resulting compounds to combat Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) superbugs. The antibacterial efficacy of <b>5(a–e)</b> moieties against MRSA was evaluated. The <b>5c</b> moiety shows a value of 29 µM and 15.08 ± 0.05 zone of inhibition (ZOI) in mm, which is lower than the minimum inhibitory concentration (MIC) value of streptomycin, which is 17 μM (18.16 ± 0.08) ZOI in mm). An in-silico docking study on the protein 3SRW of MRSA confirmed that the biocidal properties were effective against MRSA. The findings that were gathered made it very evident that <b>5c</b> had a significantly greater docking score, and a stronger binding affinity. To verify the antibacterial activity, SEM, potassium efflux, cellular leakage, and an inhibitory effect on the electron transport chain were employed. HEK 293 cell lines were used to evaluate the <b>5c</b> analogue’s cytotoxicity, and its behaviour under haemostatic circumstances was well-established. As a prospective antibacterial competitor against MRSA, <b>5c</b> analogue has the potential to be a cutting-edge medication for the complete eradication of MRSA infections, according to the data obtained.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 3","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143396550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome and pathogenicity analysis of Helicobacter mastomyrinus isolated from mice","authors":"Zhu Liqi,&nbsp;Liang Yuanyuan,&nbsp;Yang Linghan,&nbsp;Yin Jun,&nbsp;Wang Tao,&nbsp;Zhang Quan","doi":"10.1007/s00203-025-04254-x","DOIUrl":"10.1007/s00203-025-04254-x","url":null,"abstract":"<div><p>The increasing attention given to the potential risk offered by enterohepatic <i>Helicobacter</i> species to the well-being of human beings and animals is of significant importance. <i>Helicobacter mastomyrinus</i> (<i>H. mastomyrinus</i>), a bacterium predominantly associated with rodents, has been implicated in liver and intestinal pathologies. Here, a strain of <i>H. mastomyrinus</i>, designated as Hm-17 (GenBank: CP145316.1), was isolated from asymptomatic C57BL/6 mice. Subsequently, an in-depth and comprehensive investigation was undertaken, which included genome sequencing analysis, micro-biochemical identification, evaluation of growth characteristic, cytotoxicity assessment, and testing of animal pathogenicity. The analysis of 16 S rRNA reveals a close phylogenetic relationship between <i>H. mastomyrinus</i> and <i>H. canadensis</i>. However, core-pan genome analysis and an evaluation of pathogenic factors indicates a more robust association between <i>H. mastomyrinus</i> and <i>H. hepaticus</i>. Cytotoxicity analysis revealed that Hm-17 exhibits robust cytolethal distending toxin (CDT) activity, inducing pronounced cellular swelling and death. Furthermore, Hm-17 infection in BALB/c mice results in rapid and characteristic focal necrotic hepatitis. Genome sequencing and pathogenicity analysis indicate that <i>H. mastomyrinus</i> isolates from asymptomatic mice possess significant pathogenic potential. These findings underscore the need for further investigation into the epidemiology and mechanisms of pathogenesis associated with this organism.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 3","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143396454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}