Archives of Microbiology最新文献_第8页

Retraction Note: Gorillibacterium timonense sp. nov., isolated from an obese patient 撤稿说明：从一名肥胖患者体内分离出的Gorillibacterium timonense sp.

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-09-26 DOI: 10.1007/s00203-024-04146-6

Sokhna Ndongo, Mamadou Beye, Noémie Labas, Fabrizio Di Pinto, Magali Richez, Jean-Christophe Lagier, Pierre-Edouard Fournier, Didier Raoult, Fadi Bittar

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Role of grass endophytic fungi as a natural resource of bioactive metabolites 草内生真菌作为生物活性代谢物自然资源的作用。

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-09-26 DOI: 10.1007/s00203-024-04132-y

R. Nischitha

{"title":"Role of grass endophytic fungi as a natural resource of bioactive metabolites","authors":"R. Nischitha","doi":"10.1007/s00203-024-04132-y","DOIUrl":"10.1007/s00203-024-04132-y","url":null,"abstract":"<div>Grass endophytic fungi have garnered increasing attention as a prolific source of bioactive metabolites with potential application across various fields, including pharmaceticals agriculture and industry. This review paper aims to synthesize knowledge on the diversity, isolation, and bioactivity of metabolites produced by grass endophytic fungi. Additionally, this approach aids in the conservation of rare and endangered plant species. Advanced analytical techniques such as high-performance liquid chromatography, liquid chromatograpy-mass spectrometry and gas chromatography are discussed as critical tools for metabolite identification and characterization. The review also highlights significant bioactive metabolites discovered to date, emphasizing their antimicrobial, antioxidant, and insecticidal activities and plant growth regulation properties. Besides address the challenges and future prospects in harnessing grass endophytic fungi for sustainable biotenological applications. By consolidating recent advancements and identifying agaps in the current research, this paper provides a comprehensive overview of the potential grass endophytic fungi as a valuable resource for novel bioactive compounds.</div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 10","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142340029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retraction Note: Gemella massiliensis sp. nov., a new bacterium isolated from the human sputum 撤稿说明：Gemella massiliensis sp.

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-09-26 DOI: 10.1007/s00203-024-04147-5

Maxime Descartes Mbogning Fonkou, Cheikh Ibrahima Lo, Zouina Mekhalif, Melhem Bilen, Enora Tomei, Edmond Kuete Yimagou, Grégory Dubourg, Didier Raoult, Florence Fenollar, Pierre-Edouard Fournier

引用次数: 0

Genomic identification of Oryctes rhinoceros nudivirus isolates, a biocontrol agent for coconut rhinoceros beetle 椰子犀角金龟子生物控制剂 Oryctes rhinoceros nudivirus 分离物的基因组鉴定。

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-09-26 DOI: 10.1007/s00203-024-04116-y

Ela Hiszczynska-Sawicka, Mitchell K. Weston, Aurelie Laugraud, Charles A. Hefer, Jeanne M. E. Jacobs, Sean D. G. Marshall

{"title":"Genomic identification of Oryctes rhinoceros nudivirus isolates, a biocontrol agent for coconut rhinoceros beetle","authors":"Ela Hiszczynska-Sawicka, Mitchell K. Weston, Aurelie Laugraud, Charles A. Hefer, Jeanne M. E. Jacobs, Sean D. G. Marshall","doi":"10.1007/s00203-024-04116-y","DOIUrl":"10.1007/s00203-024-04116-y","url":null,"abstract":"<div>The coconut rhinoceros beetle (Oryctes rhinoceros, CRB) is a serious pest of coconut and oil palms. It is native to South and Southeast Asia and was inadvertently introduced to Samoa in 1909. It has invaded many other Pacific countries throughout the last century. Oryctes rhinoceros nudivirus (OrNV), a natural pathogen of CRB in its native range, was successfully introduced as a classical biocontrol agent and has effectively suppressed invasive CRB populations for decades. However, resurgence of CRB has been recorded, with new invasions detected in several Pacific Island Countries and Territories. Additionally, new populations of CRB are emerging in some invaded areas that have a degree of resistance to the virus isolates commonly released for CRB biocontrol. Here, we designed a fast and reliable tool for distinguishing between different OrNV isolates that can help with the selection process to identify effective isolates for management of new CRB invasions. A comparison of 13 gene/gene region sequences within the OrNV genome of 16 OrNV isolates from native and invaded ranges allowed us to identify unique Single Nucleotide Polymorphisms (SNPs). With these SNPs, we developed an assay using multiplex PCR-amplicon-based nanopore sequencing to distinguish between OrNV isolates. We found that as few as four gene fragments were sufficient to identify 15 out of 20 OrNV isolates. This method can be used as a tool to monitor the establishment and distribution of OrNV isolates selected for release as biocontrol agents in CRB-infected areas.</div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 10","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11427517/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142340069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening of promising molecules against potential drug targets in Yersinia pestis by integrative pan and subtractive genomics, docking and simulation approach 通过整合泛基因组学和减基因组学、对接和模拟方法，筛选针对鼠疫耶尔森菌潜在药物靶点的有前景分子。

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-09-25 DOI: 10.1007/s00203-024-04140-y

Lei Chen, Lihu Zhang, Yanping Li, Liang Qiao, Suresh Kumar

{"title":"Screening of promising molecules against potential drug targets in Yersinia pestis by integrative pan and subtractive genomics, docking and simulation approach","authors":"Lei Chen, Lihu Zhang, Yanping Li, Liang Qiao, Suresh Kumar","doi":"10.1007/s00203-024-04140-y","DOIUrl":"10.1007/s00203-024-04140-y","url":null,"abstract":"<div>This study focuses on Yersinia pestis, the bacterium responsible for plague, which posed a severe threat to public health in history. Despite the availability of antibiotics treatment, the emergence of antibiotic resistance in this pathogen has increased challenges of controlling the infections and plague outbreaks. The development of new drug targets and therapies is urgently needed. This research aims to identify novel protein targets from 28 Y. pestis strains by the integrative pan-genomic and subtractive genomics approach. Additionally, it seeks to screen out potential safe and effective alternative therapies against these targets via high-throughput virtual screening. Targets should lack homology to human, gut microbiota, and known human ‘anti-targets’, while should exhibit essentiality for pathogen’s survival and virulence, druggability, antibiotic resistance, and broad spectrum across multiple pathogenic bacteria. We identified two promising targets: the aminotransferase class I/class II domain-containing protein and 3-oxoacyl-[acyl-carrier-protein] synthase 2. These proteins were modeled using AlphaFold2, validated through several structural analyses, and were subjected to molecular docking and ADMET analysis. Molecular dynamics simulations determined the stability of the ligand-target complexes, providing potential therapeutic options against Y. pestis.</div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 10","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142340030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic and molecular characterization of fit95 mutation of Escherichia coli: evidence that fit95 is an allele of pheT 大肠杆菌 fit95 突变的遗传和分子特征：fit95 是 pheT 等位基因的证据。

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-09-24 DOI: 10.1007/s00203-024-04127-9

Praveen Belagal

{"title":"Genetic and molecular characterization of fit95 mutation of Escherichia coli: evidence that fit95 is an allele of pheT","authors":"Praveen Belagal","doi":"10.1007/s00203-024-04127-9","DOIUrl":"10.1007/s00203-024-04127-9","url":null,"abstract":"<div>The originally identified transcription-defective fitA76 temperature-sensitive (Ts) mutation defined an allele of pheS. Both fitA/pheS and fitB/pheT were previously proposed to function as transcription factors. Sequencing pheS region of the fitA76 mutant revealed the same G293→A293 transition found in the translation-defective pheS5 mutant. It was subsequently found that fitA76 harbored a second mutation (fit95) in addition to pheS5 mutation. The fit95 was found to be Ts on –salt media but was found unstable. In this investigation, genetic, physiological and molecular characterization of the fit95 mutation was carried out. The fit95 was genetically re-separated from the pheS5 mutation present in the fitA76 mutant and the same was subsequently mobilized into multiple genetic backgrounds to study its phenotypic modulations by altering the medium and supplements. Based on genetic studies, the unstable –salt Ts phenotype of the fit95 could be stabilized by the presence of rpoB201 mutation. Addition of glucose enhanced Ts phenotype in the presence of rpoB201 mutation, but citrate completely alleviated the Ts phenotype. Further, by series of complementation analyses and molecular cloning, the identity of fit95 was revealed as pheT gene which is part of pheST operon.</div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 10","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142307042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Paracraurococcus lichenis sp. nov., isolated from lichen in Thailand Paracraurococcus lichenis sp.

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-09-24 DOI: 10.1007/s00203-024-04129-7

Engkarat Kingkaew, Shingo Kato, Takao Iino, Takashi Itoh, Moriya Ohkuma, Wongsakorn Phongsopitanun, Somboon Tanasupawat

{"title":"Paracraurococcus lichenis sp. nov., isolated from lichen in Thailand","authors":"Engkarat Kingkaew, Shingo Kato, Takao Iino, Takashi Itoh, Moriya Ohkuma, Wongsakorn Phongsopitanun, Somboon Tanasupawat","doi":"10.1007/s00203-024-04129-7","DOIUrl":"10.1007/s00203-024-04129-7","url":null,"abstract":"<div>A novel bacterium, designated as strain LOR1-02T and isolated from a lichen sample collected from Kham Riang Subdistrict, Kantharawichai District, Maha Sarakham Province, Thailand, underwent thorough investigation utilizing a polyphasic taxonomic approach. Strain LOR1-02T demonstrated growth within a temperature range of 20–42 °C (optimal at 30 °C), pH range of 5.0–7.5 (optimal at pH 7.0), and tolerance to 4.0% (w/v) NaCl. Phylogenetic analysis revealed its close relation to Paracraurococcus ruber JCM 9931T, with a 16S rRNA gene sequence similarity of 97.16%, placing it within the genus Paracraurococcus. The approximate genome size of strain LOR1-02T was determined to be 8.6 Mb, with a G + C content of 70.9 mol%. Additionally, ANIb, ANIm, and AAI values between the whole genomes of strain LOR1-02T and type strains were calculated as 82.6–83.4%, 86.1–86.8%, and 81.4–82.2%, respectively, while the dDDH value was determined to be 26.3–28.5% (C.I. 24.0–31.0%). The predominant fatty acids detected were C18:1ω7c and/or C18:1ω6c, C16:0, and C18:12OH. The major ubiquinone identified was Q-10, and the polar lipids included phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, diphosphatidylglycerol, along with unidentified phosphoaminolipid, lipids, and an amino lipid. Based on comprehensive phenotypic, chemotaxonomic, and genotypic characterization, it is concluded that strain LOR1-02T represents a novel species within the genus Paracraurococcus, for which the name Paracraurococcus lichenis sp. nov. is proposed. The type strain designation is LOR1-02T (= JCM 33121T = NBRC 112776T = TISTR 2503T).</div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 10","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142307043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial peptide-based strategies to overcome antimicrobial resistance 基于抗菌肽的克服抗菌药耐药性战略。

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-09-23 DOI: 10.1007/s00203-024-04133-x

Meetali Girdhar, Aparajita Sen, Arti Nigam, Jyoti Oswalia, Sachin Kumar, Rashi Gupta

{"title":"Antimicrobial peptide-based strategies to overcome antimicrobial resistance","authors":"Meetali Girdhar, Aparajita Sen, Arti Nigam, Jyoti Oswalia, Sachin Kumar, Rashi Gupta","doi":"10.1007/s00203-024-04133-x","DOIUrl":"10.1007/s00203-024-04133-x","url":null,"abstract":"<div>Antibiotic resistance has emerged as a global threat, rendering the existing conventional treatment strategies ineffective. In view of this, antimicrobial peptides (AMPs) have proven to be potent alternative therapeutic interventions with a wide range of applications in clinical health. AMPs are small peptides produced naturally as a part of the innate immune responses against a broad range of bacterial, fungal and viral pathogens. AMPs present a myriad of advantages over traditional antibiotics, including their ability to target multiple sites, reduced susceptibility to resistance development, and high efficacy at low doses. These peptides have demonstrated notable potential in inhibiting microbes resistant to traditional antibiotics, including the notorious ESKAPE pathogens, recognized as the primary culprits behind nosocomial infections. AMPs, with their multifaceted benefits, emerge as promising candidates in the ongoing efforts to combat the escalating challenges posed by antibiotic resistance. This in-depth review provides a detailed discussion on AMPs, encompassing their classification, mechanism of action, and diverse clinical applications. Focus has been laid on combating newly emerging drug-resistant organisms, emphasizing the significance of AMPs in mitigating this pressing challenge. The review also illuminates potential future strategies that may be implemented to improve AMP efficacy, such as structural modifications and using AMPs in combination with antibiotics and matrix-inhibiting compounds.<h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 10","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142279793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Population distribution characteristics of mating type genes and genetic stability in Morchella sextelata 交配型基因的种群分布特征和六角森氏杆菌的遗传稳定性。

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-09-23 DOI: 10.1007/s00203-024-04141-x

Chunfeng Mu, Chen Hao, Lunhe You, Yichun Wang, Sheng Qiang, Yu Liu, Jianrui Wang

{"title":"Population distribution characteristics of mating type genes and genetic stability in Morchella sextelata","authors":"Chunfeng Mu, Chen Hao, Lunhe You, Yichun Wang, Sheng Qiang, Yu Liu, Jianrui Wang","doi":"10.1007/s00203-024-04141-x","DOIUrl":"10.1007/s00203-024-04141-x","url":null,"abstract":"<div>The reproductive mode of morels (Morchella spp.) is governed by mating type genes, specifically MAT1-1 and MAT1-2. This study investigated the presence of mating type genes at various growth stages and in different parts of cultivated Morchella sextelata. This study revealed significant fluctuations in the detection ratio of the two mating type genes during ascocarps growth. Single ascospore strains with MAT1-1, MAT1-2 and both mating types were selected for experimentations. Stress stimuli including H2O2, Congo red and NaCl were introduced into the medium. Differences in the cultural and physiological characteristics of single spore strains were analyzed, and mating type genes were identified after subculturing to assess their stability. The results indicated that a total of 297 samples with a single mating type gene were detected in 480 samples selected from the five stages of fruiting body growth, accounting for 61.9%. Stress exposure influenced colony morphology, mycelial growth rate, and biomass, leading to significant increases in malondialdehyde content and osmotic adjustment compounds, including soluble protein and proline. Physiological and biochemical parameters varied among the three mating type strains under different stress conditions. Principal component analysis was used to calculate the weight values, which showed that the MAT1-2 strain exhibited the highest tolerance to chemical stresses, particularly oxidative stress. Subculturing under stress revealed that single mating type strains ceased growth by the 8th generation, whereas both mating type strains could continue to the 15th generation without loss of mating type genes, indicating broader environmental adaptability and higher viability. These findings offer novel insights into mating type gene function and serve as a scientific foundation for the development of high-yield, stress-resistant morel varieties.</div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 10","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142307044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenotypic and genotypic perspectives on detection methods for bacterial antimicrobial resistance in a One Health context: research progress and prospects 从表型和基因型角度看 "一体健康 "背景下细菌抗菌药耐药性的检测方法：研究进展与前景。

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-09-20 DOI: 10.1007/s00203-024-04131-z

Bingbing Yang, Xiaoqi Xin, Xiaoqing Cao, Lubanga Nasifu, Zhenlin Nie, Bangshun He

{"title":"Phenotypic and genotypic perspectives on detection methods for bacterial antimicrobial resistance in a One Health context: research progress and prospects","authors":"Bingbing Yang, Xiaoqi Xin, Xiaoqing Cao, Lubanga Nasifu, Zhenlin Nie, Bangshun He","doi":"10.1007/s00203-024-04131-z","DOIUrl":"10.1007/s00203-024-04131-z","url":null,"abstract":"<div>The widespread spread of bacterial antimicrobial resistance (AMR) and multidrug-resistant bacteria poses a significant threat to global public health. Traditional methods for detecting bacterial AMR are simple, reproducible, and intuitive, requiring long time incubation and high labor intensity. To quickly identify and detect bacterial AMR is urgent for clinical treatment to reduce mortality rate, and many new methods and technologies were required to be developed. This review summarizes the current phenotypic and genotypic detection methods for bacterial AMR. Phenotypic detection methods mainly include antimicrobial susceptibility tests, while genotypic detection methods have higher sensitivity and specificity and can detect known or even unknown drug resistance genes. However, most of the current tests are either genotypic or phenotypic and rarely combined. Combining the advantages of phenotypic and genotypic methods, combined with the joint application of multiple rapid detection methods may be the trend for future AMR testing. Driven by rapid diagnostic technology, big data analysis, and artificial intelligence, detection methods of bacterial AMR are expected to constantly develop and innovate. Adopting rational detection methods and scientific data analysis can better address the challenges of bacterial AMR and ensure human health and social well-being.</div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 10","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142279796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0