Statistical factorial design for optimum reduction of tellurite and production of tellurium nanostructure by a novel strain Phytobacter diazotrophicus Te1

Abstract

A tellurite-reducing isolate (Te1) was recovered from a soil sample receiving industrial effluents from Ismailia Canal, Egypt. The isolate exhibited dark black colonies when grown on solid medium containing potassium tellurite, which indicated the reduction of tellurite to black tellurium. The isolate was identified using 16S rRNA gene sequencing and was submitted to GenBank as Phytobacter diazotrophicus strain Te1 (PP724698). The tellurite reduction percentage was 96.5% ± 0.354%. Moreover, energy-dispersive X-ray (EDX) analysis confirmed the presence of tellurium nanostructure, with a 3.7 keV absorption peak along with phosphorus, sulfur, and oxygen, revealing a complex biogenic nature. Fourier-transform infrared (FTIR) spectroscopy identified distinct absorption peaks within the 400–4000 cm⁻¹ range, corresponding to various vibrational modes of chemical bonds, including those of lipids, proteins, polysaccharides, and free radicals. X-ray diffraction (XRD) analysis highlighted the nanoscale crystalline structure of the material, with broad peaks confirming limited crystallite size and structural disorder, and revealed tellurium peaks on a hexagonal phase at 2-theta values of 27.36°, 38.19° and 40.20°. According to the results of the response optimizer and the subsequent validation experiments, complete reduction of tellurium was achieved at a medium pH of 6.8, incubation temperature of 33.5 °C, tellurite concentration of 1375 μM, and agitation speed of 110 rpm for 96 h. Black Te nanostructure was visible intracellularly and extracellularly upon examination using the transmission electron microscope. To the best of the authors’ knowledge, this is the first report of tellurite reduction by Phytobacter diazotrophicus.