Current Opinion in Chemical Biology最新文献

Chemical strategies for trapping fleeting enzymatic complexes in nucleosome ubiquitylation 在核小体泛素化中捕获稍纵即逝的酶复合物的化学策略

IF 6.1 2区生物学

Current Opinion in Chemical Biology Pub Date : 2026-02-01 Epub Date: 2026-01-07 DOI: 10.1016/j.cbpa.2025.102651

Yun Liu, Qi Shu, Huasong Ai

引用次数: 0

Bridging or exchanging partners? A supramolecular perspective on bifunctional molecules and their potential for triggerable enzyme therapy 架桥还是交换伙伴？双功能分子的超分子视角及其在可触发酶治疗中的潜力。

IF 6.1 2区生物学

Current Opinion in Chemical Biology Pub Date : 2026-02-01 Epub Date: 2026-01-06 DOI: 10.1016/j.cbpa.2025.102650

Leila Motiei, Martín López-Vidal, David Margulies

{"title":"Bridging or exchanging partners? A supramolecular perspective on bifunctional molecules and their potential for triggerable enzyme therapy","authors":"Leila Motiei, Martín López-Vidal, David Margulies","doi":"10.1016/j.cbpa.2025.102650","DOIUrl":"10.1016/j.cbpa.2025.102650","url":null,"abstract":"<div><div>Bifunctional molecules are commonly regarded as proximity-inducing compounds (PICs) that bridge two proteins to generate novel biological outcomes. Here, we highlight a distinct and emerging subclass, termed partner-exchanging molecules (PEMs), which cannot bind both protein partners simultaneously. Instead, they act through a partner-swapping mechanism, enabling one protein to unnaturally activate another. This perspective examines their mechanisms of action and surveys potential applications such as protein sensing, prodrug activation, controlled release, and the reconfiguration of intracellular signaling. Because PEMs offer a simple route to build triggerable enzymes, we further discuss them in the context of artificial zymogens, emphasizing their promise to mediate activation of therapeutic proenzyme systems via non-enzyme proteins and to render native enzymes triggerable in cells. By positioning PEMs as a conceptual link between bifunctional molecules and artificial zymogens, we aim to broaden our understanding of bifunctional regulators and expand the scope of artificial proenzyme design.</div></div>","PeriodicalId":291,"journal":{"name":"Current Opinion in Chemical Biology","volume":"90 ","pages":"Article 102650"},"PeriodicalIF":6.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145916338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Small RNAs, big potential: Engineering microRNA-based synthetic gene circuits 小rna，大潜力：基于微rna的合成基因电路工程

IF 6.1 2区生物学

Current Opinion in Chemical Biology Pub Date : 2026-02-01 Epub Date: 2026-01-31 DOI: 10.1016/j.cbpa.2026.102652

Archismita Kundu , Roman Jerala

{"title":"Small RNAs, big potential: Engineering microRNA-based synthetic gene circuits","authors":"Archismita Kundu , Roman Jerala","doi":"10.1016/j.cbpa.2026.102652","DOIUrl":"10.1016/j.cbpa.2026.102652","url":null,"abstract":"<div><div>MicroRNAs (miRs) are small non-coding RNAs that regulate gene expression. Their dysregulation is closely associated with various diseases, positioning them as biomarkers of cellular state. Synthetic biology has leveraged these properties to engineer miR-based genetic circuits capable of sensing and interpreting endogenous miR levels. Early miR-OFF systems relied on reporter gene repression but were limited by ambiguous signal interpretation. Subsequent advances introduced miR-ON architectures, logic-based classifiers integrating multiple miRs, and layered regulatory strategies combining transcriptional, translational, and cleavage-based modules to enhance sensitivity and specificity. Recent innovations include CRISPR-associated miR-responsive systems and incoherent feed-forward loop (iFFL) architectures that stabilize gene expression amid cellular variability, shifting applications from passive sensing to therapeutic intervention. Despite challenges such as leakage, cellular resource resources, and delivery, progress in orthogonal miR toolkits, computational modeling, and RNA-based delivery platforms is rapidly driving miR-based circuits toward diagnostic and therapeutic applications.</div></div>","PeriodicalId":291,"journal":{"name":"Current Opinion in Chemical Biology","volume":"90 ","pages":"Article 102652"},"PeriodicalIF":6.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146073750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical dissection of microbiology and microbiota 微生物学和微生物群的化学解剖

IF 6.1 2区生物学

Current Opinion in Chemical Biology Pub Date : 2026-02-01 Epub Date: 2025-12-24 DOI: 10.1016/j.cbpa.2025.102641

Catherine L. Grimes, Howard C. Hang

引用次数: 0

Synthetic biology for phytohormone production 植物激素合成生物学

IF 6.1 2区生物学

Current Opinion in Chemical Biology Pub Date : 2026-02-01 Epub Date: 2025-12-27 DOI: 10.1016/j.cbpa.2025.102649

Pattarawan Intasian , Ninlapan Kimprasoot , Duangthip Trisrivirat, Pimchai Chaiyen

引用次数: 0

Emerging trends in chemoproteomics: Mapping the landscape of protein–metabolite interactions 化学蛋白质组学的新趋势：绘制蛋白质-代谢物相互作用的景观

IF 6.1 2区生物学

Current Opinion in Chemical Biology Pub Date : 2025-12-01 Epub Date: 2025-09-25 DOI: 10.1016/j.cbpa.2025.102631

Ning Wan , Chenguang Liu , Haiping Hao , Hui Ye

引用次数: 0

Editorial Overview: Converging innovations in chemical proteomics 编辑概述：化学蛋白质组学的融合创新。

IF 6.1 2区生物学

Current Opinion in Chemical Biology Pub Date : 2025-12-01 Epub Date: 2025-11-19 DOI: 10.1016/j.cbpa.2025.102640

Jing Yang, Wenqing Shui

引用次数: 0

Evolving advances of proximity labeling in capturing biomolecular interactions 接近标记在捕获生物分子相互作用中的发展进展

IF 6.1 2区生物学

Current Opinion in Chemical Biology Pub Date : 2025-10-01 Epub Date: 2025-09-11 DOI: 10.1016/j.cbpa.2025.102629

Ting Dang , Wenqing Shui

引用次数: 0

In vivo cross-linking mass spectrometry: Advances and challenges in decoding protein conformational dynamics and complex regulatory networks in living cells 体内交联质谱：解码活细胞中蛋白质构象动力学和复杂调节网络的进展和挑战

IF 6.1 2区生物学

Current Opinion in Chemical Biology Pub Date : 2025-10-01 Epub Date: 2025-09-12 DOI: 10.1016/j.cbpa.2025.102630

Jing Chen , Qun Zhao , Yukui Zhang , Lihua Zhang

{"title":"In vivo cross-linking mass spectrometry: Advances and challenges in decoding protein conformational dynamics and complex regulatory networks in living cells","authors":"Jing Chen , Qun Zhao , Yukui Zhang , Lihua Zhang","doi":"10.1016/j.cbpa.2025.102630","DOIUrl":"10.1016/j.cbpa.2025.102630","url":null,"abstract":"<div><div><em>In vivo</em> chemical cross-linking mass spectrometry (XL-MS) has emerged as a powerful technique for high-throughput, proteome-wide mapping of intramolecular conformations and intermolecular interactions of protein complexes in living cells. By providing distance constraints between specific residues, XL-MS enables the characterization of protein conformations and interaction networks under near-physiological conditions, greatly facilitating the analysis of biomacromolecular functions and regulatory mechanisms. The information obtained from cross-linking is particularly valuable at the systems level, and its value continues to increase with improvements in the density of cross-link identification, the precision of distance constraints, and the spatiotemporal resolution. In recent years, advances in cross-linker design, cross-linked peptide enrichment methods, mass spectrometry analysis, and artificial intelligence-assisted data analysis have significantly expanded the capabilities of <em>in vivo</em> XL-MS. This article systematically reviews the latest progress in <em>in vivo</em> XL-MS for protein conformation and interaction network analysis, highlights its unique advantages, discusses current technical challenges, and explores further development.</div></div>","PeriodicalId":291,"journal":{"name":"Current Opinion in Chemical Biology","volume":"88 ","pages":"Article 102630"},"PeriodicalIF":6.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145044512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mass spectrometry-based characterization of histone post-translational modification 基于质谱的组蛋白翻译后修饰表征

IF 6.9 2区生物学

Current Opinion in Chemical Biology Pub Date : 2025-10-01 Epub Date: 2025-07-24 DOI: 10.1016/j.cbpa.2025.102622

Wensi Zhao , Jun Zhang , Kaifeng Chen , Jian Yuan , Linhui Zhai , Minjia Tan

{"title":"Mass spectrometry-based characterization of histone post-translational modification","authors":"Wensi Zhao , Jun Zhang , Kaifeng Chen , Jian Yuan , Linhui Zhai , Minjia Tan","doi":"10.1016/j.cbpa.2025.102622","DOIUrl":"10.1016/j.cbpa.2025.102622","url":null,"abstract":"<div><div>Histone post-translational modifications (PTMs) play critical roles in regulating chromatin dynamics and gene expression. Increasing evidence demonstrates that the dysregulation of histone PTMs is closely associated with the pathogenesis of various diseases. Traditional methods for detecting histone PTMs, such as western blot (WB) and chromatin immunoprecipitation sequencing (ChIP-seq), are often limited by their dependence on specific antibodies and relatively low analytical throughput. Mass spectrometry (MS)-based proteomics offers a powerful and unbiased approach for comprehensive characterization of histone PTMs. This review focuses on the advanced development of MS-based strategies for characterizing histone PTMs. These strategies include histone extraction, enzymatic digestion, labeling, enrichment, and MS-based detection. These techniques not only enable comprehensive identification and quantitative analysis of classical modifications, such as acetylation and methylation, but also substantially facilitate the discovery of less-characterized histone PTMs, including succinylation, lactylation, crotonylation, and monoaminylation. Consequently, these findings significantly enhance the complexity of histone code. Collectively, MS-based approaches have profoundly advanced our understanding of histone PTM landscapes and their potential epigenetic regulatory mechanisms in both physiology and pathology contexts.</div></div>","PeriodicalId":291,"journal":{"name":"Current Opinion in Chemical Biology","volume":"88 ","pages":"Article 102622"},"PeriodicalIF":6.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0