Current Opinion in Chemical Biology最新文献_第2页

Recent advances in chemical proteomics for protein profiling and targeted degradation 化学蛋白质组学在蛋白质分析和靶向降解方面的最新进展

IF 6.9 2区生物学

Current Opinion in Chemical Biology Pub Date : 2025-06-26 DOI: 10.1016/j.cbpa.2025.102605

Peng-Kai Liu , Zicong Wang , Lingjun Li

{"title":"Recent advances in chemical proteomics for protein profiling and targeted degradation","authors":"Peng-Kai Liu , Zicong Wang , Lingjun Li","doi":"10.1016/j.cbpa.2025.102605","DOIUrl":"10.1016/j.cbpa.2025.102605","url":null,"abstract":"<div><div>Chemical proteomics has emerged as a powerful approach to decipher protein function, interactions, and targeted degradation pathways in complex biological systems. Recent advances in chemical labeling strategies, including activity-based protein profiling (ABPP), proximity labeling (PL), and proteolysis-targeting chimeras (PROTACs), have facilitated a deeper understanding of protein function and interaction networks. First, ABPP employs covalent probes to selectively label active enzymes, uncovering functional proteomics and drug–target interactions. Innovations such as PhosID-ABPP and streamlined cysteine ABPP have improved site-specific quantification and throughput, enabling proteome-wide analysis of enzyme activity and small-molecule interactions. Second, PL enables the characterization of transient protein–protein interactions using enzymatic or chemically triggered approaches. Advances including TurboID and TransitID enhanced the spatiotemporal resolution of PL. Third, PROTACs expand the scope of targeted protein degradation by leveraging the ubiquitin–proteasome system. Collectively, we highlight recent advancements in integrating mass spectrometry (MS) with these methodologies in the field of chemical proteomics.</div></div>","PeriodicalId":291,"journal":{"name":"Current Opinion in Chemical Biology","volume":"87 ","pages":"Article 102605"},"PeriodicalIF":6.9,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144481106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in metallophore research uncover functions in quorum sensing, antimicrobial activity, and lanthanide acquisition 最近的研究进展揭示了金属团在群体感应、抗菌活性和镧系元素获取方面的功能

IF 6.9 2区生物学

Current Opinion in Chemical Biology Pub Date : 2025-06-19 DOI: 10.1016/j.cbpa.2025.102604

Ghazal Kamyabi, Erica L. Debley, Elizabeth M. Nolan

引用次数: 0

Ion transport across biological membranes 离子在生物膜上的传输

IF 6.9 2区生物学

Current Opinion in Chemical Biology Pub Date : 2025-06-18 DOI: 10.1016/j.cbpa.2025.102606

Robin S. Bon, Matthew J. Langton

引用次数: 0

Chemical tools to study and modulate glycan-mediated host-bacteria interactions 研究和调节聚糖介导的宿主-细菌相互作用的化学工具

IF 6.9 2区生物学

Current Opinion in Chemical Biology Pub Date : 2025-06-04 DOI: 10.1016/j.cbpa.2025.102603

Divya Bhargava , Aniqa Chowdhury , Danielle H. Dube

引用次数: 0

Probing ligand-induced local stability shifts: A sensitive approach to identify target proteins and binding sites at the proteomic scale 探测配体诱导的局部稳定性转移：一种在蛋白质组学尺度上识别靶蛋白和结合位点的敏感方法

IF 6.9 2区生物学

Current Opinion in Chemical Biology Pub Date : 2025-05-31 DOI: 10.1016/j.cbpa.2025.102602

Keyun Wang , Yanan Li , Yanni Ma , Mingliang Ye

{"title":"Probing ligand-induced local stability shifts: A sensitive approach to identify target proteins and binding sites at the proteomic scale","authors":"Keyun Wang , Yanan Li , Yanni Ma , Mingliang Ye","doi":"10.1016/j.cbpa.2025.102602","DOIUrl":"10.1016/j.cbpa.2025.102602","url":null,"abstract":"<div><div>Deciphering ligand-induced structural alterations represents a critical frontier in functional proteomics, offering transformative potential for both target identification and mechanistic understanding of ligand actions. Therefore, it is of great interest to develop robust proteomic approaches to uncover the binding relationship between ligands and the targeting proteins. The ligand modification-free approaches have the advantages of bypassing chemical modification and thus are broadly applicable to diverse ligands. Herein, in this review, we will introduce some recent methodology advancements for modification-free approaches in the identification of interactions between ligands and proteins. Especially, we will focus on a newly developed method, peptide-centric local stability assay (PELSA), which was designed to probe the ligand-induced local stability shifts and was demonstrated to have high sensitivity in revealing the binding regions at proteome level. The continued refinement and wider implementation of such technologies are expected to revolutionize fundamental research related with ligand–protein interactions.</div></div>","PeriodicalId":291,"journal":{"name":"Current Opinion in Chemical Biology","volume":"87 ","pages":"Article 102602"},"PeriodicalIF":6.9,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144184648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Natural and artificial siderophores: Iron-based applications and beyond 天然和人工铁载体：铁基应用及其他

IF 6.9 2区生物学

Current Opinion in Chemical Biology Pub Date : 2025-05-23 DOI: 10.1016/j.cbpa.2025.102601

Zih-Jheng Lin, Cheng-Yu Fang, Tsung-Shing Andrew Wang

引用次数: 0

Recent developments in probing the levels and flux of selected organellar cations as well as organellar mechanosensitivity 在探测选定的细胞器阳离子的水平和通量以及细胞器的机械敏感性方面的最新进展

IF 6.9 2区生物学

Current Opinion in Chemical Biology Pub Date : 2025-05-03 DOI: 10.1016/j.cbpa.2025.102600

Taufiq Rahman , Sandip Patel

{"title":"Recent developments in probing the levels and flux of selected organellar cations as well as organellar mechanosensitivity","authors":"Taufiq Rahman , Sandip Patel","doi":"10.1016/j.cbpa.2025.102600","DOIUrl":"10.1016/j.cbpa.2025.102600","url":null,"abstract":"<div><div>Electrochemical gradients exist not only across the plasma membrane (PM) but also across membranes of organelles. Various endomembrane-localised ion channels and transporters have been identified, the activity of which is critical for organellar (and also cellular) ionic homeostasis that underpins diverse cellular processes. Aberrant organellar ion flux underlies several diseases, identifying organellar channels and transporters as potential drug targets. Therefore, the need for probing the functions of these proteins in situ cannot be overemphasised. The acidic interior of a few organelles as well as the dynamic nature of most organelles historically presented challenges for reliable estimation of luminal ionic concentrations. But there have been significant methodological and technical advancements by now, allowing measurement of levels of specific ions within these organelles as well as their flux across endomembranes with increasing precision. Evidence also continues to amass reporting mechanosensitivity of the endomembranes and its physiological significance. Here we highlight some recent developments in tools and techniques for measuring the levels and movement of some selected organellar cations as well as organellar mechanosensitivity.</div></div>","PeriodicalId":291,"journal":{"name":"Current Opinion in Chemical Biology","volume":"87 ","pages":"Article 102600"},"PeriodicalIF":6.9,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143900426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial overview: Transforming intracellular delivery: Innovations in macromolecule transport 编辑概述：转化细胞内递送：大分子运输的创新

IF 6.9 2区生物学

Current Opinion in Chemical Biology Pub Date : 2025-04-28 DOI: 10.1016/j.cbpa.2025.102599

Jean-Philippe Pellois

引用次数: 0

Editorial: Unusual enzyme reactions in natural products biosynthesis 社论：天然产物生物合成中不寻常的酶反应

IF 6.9 2区生物学

Current Opinion in Chemical Biology Pub Date : 2025-04-26 DOI: 10.1016/j.cbpa.2025.102597

Taifo Mahmud, Ikuro Abe

引用次数: 0

Chemical strategies for targeting lipid pathways in bacterial pathogens 针对细菌病原体脂质途径的化学策略