Anaerobe最新文献

{"title":"Clostridioides difficile Infection and Testing Rates in South Africa: A multicentre study, 2017-2020.","authors":"Trusha Nana, Praksha Ramjathan, Khine Swe-Swe Han, Kessendri Reddy","doi":"10.1016/j.anaerobe.2024.102937","DOIUrl":"https://doi.org/10.1016/j.anaerobe.2024.102937","url":null,"abstract":"<p><strong>Objectives: </strong>To describe Clostridioides difficile infection (CDI) rates and testing practices, at three tertiary/quaternary hospitals in South Africa (SA) for the period 2017 to 2020.</p><p><strong>Methods: </strong>A retrospective laboratory record review of all C. difficile testing at the Charlotte Maxeke Johannesburg Academic Hospital (CMJAH), Tygerberg Hospital (TBH) and Inkosi Albert Luthuli Central Academic Hospital (IALCH) was performed. Clinical records of patients with rCDI were reviewed to determine recurrent CDI (rCDI) rates.</p><p><strong>Results: </strong>The median primary CDI rates per 10,000 patient-days (PD) were 5.3 at CMJAH, 1.8 at TBH, and 0.3 at IALCH. In 2020, all hospitals reported an increase in primary CDI rates compared to 2019. The median testing rates per 10,000 PD were 39 at CMJAH, 14 at TBH, and 4 at IALCH. The median age of patients with primary CDI was 33 years (IQR: 22-45 years). The rCDI rates ranged from 2 to 5 per 100 incident episodes.</p><p><strong>Conclusion: </strong>Significant variations in CDI and testing rates were observed across the three hospitals. An increase in CDI rates was noted at all centres during the 2020 SARS-CoV-2 outbreak. Advanced age was not prevalent in the cohort, and rCDI rates were relatively low. These findings highlight the need for systematic surveillance of healthcare-onset CDI across SA hospitals.</p>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":" ","pages":"102937"},"PeriodicalIF":2.5,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142875924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fusobacterium necrophorum septic arthritis of the hip: A case-report and literature review.","authors":"Giacomo Franceschi, Mattia Marchi, Francesco Zambianchi, Marianna Meschiari, Cristina Mussini, Andrea Bedini","doi":"10.1016/j.anaerobe.2024.102934","DOIUrl":"10.1016/j.anaerobe.2024.102934","url":null,"abstract":"<p><strong>Introduction: </strong>Fusobacterium necrophorum is a rare but significant cause of septic arthritis, typically following oropharyngeal infections in adolescents. This anaerobic pathogen, commonly associated with Lemierre's syndrome, can lead to joint infections, posing risks for severe morbidity if diagnosis and treatment are delayed. Awareness and timely intervention are essential for preventing long-term joint damage.</p><p><strong>Case report: </strong>We report the case of a 19-year-old woman who developed high fever and acute right hip pain one week after a sore throat. Imaging revealed septic arthritis, with F. necrophorum identified in both blood and synovial fluid cultures. She received intravenous piperacillin/tazobactam, followed by outpatient parenteral therapy through an elastomeric pump, achieving full recovery. This case adds to the 42 cases documented in our literature review, reinforcing the need for prompt antimicrobial therapy.</p><p><strong>Conclusion: </strong>Fusobacterium-induced septic arthritis, though uncommon, should be considered in young patients presenting with joint infections post-pharyngitis. Early diagnosis and targeted antimicrobial therapy, particularly with β-lactamase inhibitors, are critical for effective management and preventing joint sequelae.</p>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":" ","pages":"102934"},"PeriodicalIF":2.5,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142817076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Description of Anaerococcus kampingiae sp. nov., Anaerococcus groningensis sp. nov., Anaerococcus martiniensis sp. nov., and Anaerococcus cruorum sp. nov., isolated from human clinical specimens.","authors":"K E Boiten, J Meijer, E M van Wezel, A C M Veloo","doi":"10.1016/j.anaerobe.2024.102935","DOIUrl":"https://doi.org/10.1016/j.anaerobe.2024.102935","url":null,"abstract":"<p><strong>Objectives: </strong>To improve the identification of anaerobic bacteria, the identity of clinical isolates which could not be identified using MALDI-TOF MS was assessed using whole genome sequencing (WGS) and in-house made main spectral profiles (MSPs) were created. Four novel Anaerococcus species, each represented by at least two isolates, were encountered.</p><p><strong>Methods: </strong>The novelty of the isolates was confirmed by comparing the 16S rRNA gene sequences and the WGS with their closest relatives. Phylogenetic and phylogenomic relationships were determined using MEGA X and DSMZ TYGS. Biochemical features were determined and the clustering of the created MSPs was calculated. Possible clinical relevance was assessed.</p><p><strong>Results: </strong>The novelty of the four different species was confirmed by the ANI value, and phylogenetic/phylogenomic clustering. Three of these species shared the same biochemical features, while one showed a different pattern. Only this latter species can be differentiated from other Anaerococcus spp. Remarkebly, six of the ten isolates were obtained from a positive blood culture, of which in five cases the bacterium was the only species encountered.</p><p><strong>Conclusions: </strong>We propose to name these novel species: Anaerococcus kampingiae (ENR0874^T=DSM 117234^T, CCUG 77487^T (accession numbers PP192775/ JBGMEF000000000)), Anaerococcus groningensis (ENR1011^T=DSM 117232^T, CCUG 77488^T (accession numbers PP192777/JBGMEG000000000)), Anaerococcus martiniensis (ENR0831^T=DSM 117233^T, CCUG 77486^T (accession numbers PP192776/JBGMEI000000000)), and Anaerococcus cruorum (ENR1039^T=DSM117235^T, CCUG 77489^T (accession numbers PP192778/JBGMEH000000000)).</p>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":" ","pages":"102935"},"PeriodicalIF":2.5,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142817073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Presence of Clostridioides difficile on spinach, carrots, cheese and milk in Turkey.","authors":"Gizem Taylan Yalçın, Melike Nur Tosun Demir, Gizem Korkmazer, Alper Akçalı, Nükhet Nilüfer Demirel Zorba","doi":"10.1016/j.anaerobe.2024.102933","DOIUrl":"10.1016/j.anaerobe.2024.102933","url":null,"abstract":"<p><strong>Introduction: </strong>The presence of Clostridioides difficile in water, soil, fertilizers, and animal feces suggests the potential existence of C. difficile in foods that come into contact with these sources or become contaminated through indirect means.</p><p><strong>Material & method: </strong>A total of 431 samples, consisting of spinach and carrots and raw milk and cheese obtained from cows, goats, buffalo, and sheep, were examined for the presence of C. difficile. Isolates were identified by real-time PCR, ribotyped, and their toxin profiles were determined. Antibiotic susceptibility to vancomycin, clindamycin, and metronidazole was evaluated using the E-test.</p><p><strong>Results: </strong>C. difficile was detected in 3.27 % (4/122) of spinach, 1.85 % (2/108) of carrots, and 2.19 % (2/91) of milk samples. No C. difficile was detected in the cheeses (n = 110). All isolates were obtained from different fields/farms. Only one isolate (from spinach) carried the tcdA and tcdB toxin genes. Six different PCR ribotypes were detected, with two (001, 060) being identified. All isolates were sensitive to vancomycin, clindamycin, and metronidazole.</p><p><strong>Conclusion: </strong>The prevalence of C. difficile in spinach, carrot, and milk samples from selected regions was low, and nontoxigenic strains were prevalent. Despite the low prevalence, the detection of C. difficile in these foods highlights the potential risk of foodborne transmission of this pathogen and underscores the need for monitoring and control strategies to ensure food safety.</p>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":" ","pages":"102933"},"PeriodicalIF":2.5,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genotypic and phenotypic diversity of carbapenem-resistant Bacteroides fragilis strains collected from different clinical origins.","authors":"Yanyan Wang, Juan Wen, Binxin Guo, Wenqi Zheng, Junrui Wang","doi":"10.1016/j.anaerobe.2024.102924","DOIUrl":"10.1016/j.anaerobe.2024.102924","url":null,"abstract":"<p><strong>Objective: </strong>Strains of carbapenem-resistant Bacteroides fragilis have frequently emerged in recent years. In China, data on the genotypic and phenotypic characteristics of these antimicrobial-resistant anaerobic bacteria are scarce. Thus, the aim of this study was to characterize clinical isolates of carbapenem-resistant B. fragilis collected from a tertiary hospital in China using whole genome sequencing (WGS), phenotypic susceptibility tests, and a biofilm formation assay.</p><p><strong>Methods: </strong>We analyzed 49 B. fragilis strains with different antimicrobial resistance profiles. Antimicrobial susceptibility was determined using the agar dilution method and biofilm formation using a crystal violet assay. Genomic characteristics were analyzed using WGS, and the transcription level of cfiA, which is responsible for carbapenem resistance, was determined using quantitative reverse transcription polymerase chain reaction (PCR). Carbapenem-sensitive isolates were used as controls.</p><p><strong>Results: </strong>All 49 B. fragilis isolates were biofilm producers and the percentage of carbapenem-resistant isolates was 42.86 % (21/49). The percentage of carbapenem-resistant isolates with medium-to-strong biofilm production ability was significantly lower than that of carbapenem-sensitive isolates (19.1 % vs. 88.9 %, p < 0.01). None of the carbapenem-resistant B. fragilis isolates carried bft. In contrast, 53.6 % (15/28) of the carbapenem-sensitive isolates carried bft, and all of them were fpn(+). All carbapenem-resistant isolates (21/21, 100 %) harbored cfiA and its upstream insertion sequence (IS) element. Three isolates (BF058, BF059, and BF060) carried the IS613 element, which was not immediately adjacent upstream to cfiA but was separated by a 1000-kb sequence encoding vatD. The quantitative PCR assay results revealed the elevated expression of cfiA mRNA among carbapenem-resistant isolates, although the relative expression levels varied greatly among isolates. However, a significant correlation between the relative expression level of cfiA mRNA and phenotypic carbapenem resistance was observed.</p><p><strong>Conclusions: </strong>Carbapenem-resistant B. fragilis isolates carried a low frequency of virulence-related genes and showed weaker biofilm formation ability compared with carbapenem-sensitive B. fragilis isolates. CfiA was the dominant mediator of carbapenem resistance in B. fragilis. This study was the first to identify the structural plasticity of the cfiA-IS element, emphasizing the diverse and complex evolution of carbapenem resistance in B. fragilis, which warrants further investigation.</p>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":" ","pages":"102924"},"PeriodicalIF":2.5,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New insights into the factors influencing methanogenic pathways in anaerobic digesters.","authors":"Helena Rodrigues Oliveira, Thuane Mendes Anacleto, Fernanda Abreu, Alex Enrich-Prast","doi":"10.1016/j.anaerobe.2024.102925","DOIUrl":"10.1016/j.anaerobe.2024.102925","url":null,"abstract":"<p><strong>Introduction: </strong>Anaerobic digestion integrates waste treatment, energy generation, and nutrient recycling, producing methane mainly through acetoclastic (AM) and hydrogenotrophic methanogenesis (HM). Methanogenic pathway management can improve biogas productivity and quality. The balance between pathways is influenced by environmental and physicochemical conditions, with conflicting results on the effect of different factors often reported. This systematic review aims to clarify the influence of various parameters on methanogenic pathways in anaerobic digesters.</p><p><strong>Methods: </strong>Literature search was conducted in the Web of Science and Scopus databases. The effects of different parameters on the predominant methanogenic pathway were evaluated using Kruskal-Wallis tests and Spearman's rank correlation.</p><p><strong>Results: </strong>Thermophilic temperatures and high free ammonia nitrogen concentrations (>300 mg L^-1) increase HM, with a strong combined effect of these variables. Conversely, under moderate temperature and ammonia concentrations, the primary feedstock influences the methanogenic pathway, with algae biomass, pig manure, and food industry wastewater showing the lowest contribution of hydrogenotrophic methanogens. pH effect varied with temperature, with acidic and alkaline pH favoring HM in mesophilic and thermophilic digesters, respectively. Furthermore, higher levels of volatile fatty acids (>2000 mg L^-1), carbohydrates (>10 g/L) and lipids (>10 g/L) also appeared to favor HM over AM, while most metals - especially Cr, Se and W - promoted AM.</p><p><strong>Conclusion: </strong>This study emphasizes the role of various factors in methanogenic pathway selection, highlighting the impact of previously overlooked parameters, such as inorganic elements and organic matter composition. These insights are essential for understanding the methanogenic pathway balance and optimizing biogas processes.</p>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":" ","pages":"102925"},"PeriodicalIF":2.5,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clostridioides difficile hypervirulent strain ST1 isolated from clinical stool specimens obtained from three Provinces in South Africa.","authors":"Hlambani Shirinda, Anthony M Smith, Ben Prinsloo, Marleen M Kock, Mishalan Moodley, Mohamed Said, Marthie M Ehlers","doi":"10.1016/j.anaerobe.2024.102926","DOIUrl":"10.1016/j.anaerobe.2024.102926","url":null,"abstract":"<p><strong>Objectives: </strong>Clostridioides difficile infection is a serious healthcare-associated infection linked to antimicrobial use. The severity of the disease can be associated with hypervirulent ribotypes such as RT027. The study aimed to investigate the molecular epidemiology and genomic characteristics of C. difficile isolates from private and public healthcare settings in South Africa.</p><p><strong>Methods: </strong>One hundred clinical stool specimens were cultured on cycloserine-cefoxitin-fructose agar. Conventional multiplex polymerase chain reaction (M-PCR) assays were conducted for isolate identification and detection of toxin genes. Genomic characteristics of the isolates were determined using whole genome sequencing (WGS) and data was analysed using pubMLST, EnteroBase, Pathogenwatch and CARD.</p><p><strong>Results: </strong>One hundred clinically presumptive C. difficile positive stool specimens were collected, of which 62 % (62/100) were confirmed as C. difficile by M-PCR assay. Among the 62 identified C. difficile isolates, 97 % (60/62) were toxigenic, with the most dominant toxin profile being A ⁺ B ⁺ CDT ⁺ according to the M-PCR assay. The results showed that 93 % (40/43) of the WGS analysed C. difficile strains clustered into clades 1 to 5. These 40 strains were categorized into 16 sequence types (STs), with ST1 (clade 2) being the most prevalent, representing 45 % (18/40), this strain is an RT027-associated strain previously epidemic hypervirulent strain. One major cluster (n = 18) comprising ST1 strains was identified in Gauteng Province and all the isolates associated with this cluster showed the same resistome (antimicrobial resistance genes and mutations: CDD-1, aac (6')-Ie-aph (2″)-Ia, PnimB^G and Thr82Ile). The study also identified one strain as ST11, this strain is well known for its zoonotic potential, and two strains were identified as ST37 known as an epidemic strain. Strains from public healthcare settings exhibited genetic similarity, while those from private settings showed greater genetic diversity.</p><p><strong>Conclusion: </strong>The study reported, for the first time, hypervirulent strains ST1 in Africa and ST11 in South Africa, with a minimum spanning tree indicating an ongoing ST1 outbreak.</p>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":" ","pages":"102926"},"PeriodicalIF":2.5,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitin promotes equol production via n-acetylglucosamine in human fecal cultures.","authors":"Miyuki Kodera, Kohei Nakamura, S Yokoyama","doi":"10.1016/j.anaerobe.2024.102923","DOIUrl":"10.1016/j.anaerobe.2024.102923","url":null,"abstract":"<p><strong>Objectives: </strong>Equol is a metabolite of isoflavone. Here, we investigated the effect of dietary fiber on equol-producing microbiota.</p><p><strong>Methods: </strong>Human feces was anaerobically cultured in Gifu Anaerobic Medium (GAM) containing 1 mg/L daidzein supplemented with 1 % (w/v) dietary fiber (pectin, chitin, or resistant starch [RS]). The equol conversion rate, number of equol-producing bacteria, pH, short-chain fatty acids (SCFAs), and microbial composition were measured in fecal cultures.</p><p><strong>Results: </strong>Equol conversion significantly increased by 2.7-fold in the chitin group compared with the control group. Conversely, the conversion rate decreased to one-tenth in the pectin group and one-third in the RS group. The number of equol-producing bacteria also significantly increased in the chitin group compared with the control group. Whereas pH values and acetate/propionate concentrations decreased in the pectin and RS groups compared with the control group, no significant differences were observed in the chitin group. Microbiota analysis indicated that the relative abundances of Bifidobacterium and Lactobacillus increased, whereas those of Dorea, Lachnoclostridium, and Eggerthella decreased in the pectin and RS groups. In contrast, these abundances remained stable in the chitin group. The relative abundances of Adlercreutzia and Slackia remained unchanged across all groups. In vitro cultures of Eggerthella sp. strain YY7918 and Adlercreutzia equolifaicens JCM14793 demonstrated that equol conversion from daidzein was stimulated by the addition of 0.5-1% (w/v) n-acetylglucosamine, a breakdown product of chitin, but not chitin itself.</p><p><strong>Conclusions: </strong>Our findings suggest that equol production is stimulated by n-acetylglucosamine, derived from decomposed chitin, in certain equol-producing bacteria without affecting their growth.</p>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":" ","pages":"102923"},"PeriodicalIF":2.5,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142738151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Veillonella orientalis sp. nov., an anaerobic Gram-stain-negaitve coccus isolated from saliva of a Thai child.","authors":"Izumi Mashima-Usami, Citra F Theodorea, Kiyoshi Murata, Boonyanit Thaweboon, Sroisiri Thaweboon, Futoshi Nakazawa","doi":"10.1016/j.anaerobe.2024.102921","DOIUrl":"10.1016/j.anaerobe.2024.102921","url":null,"abstract":"<p><strong>Objective: </strong>The genus Veillonella is one of the major and important constitutions of the oral microbiome. A novel anaerobic, Gram-negative coccus belonging to the genus Veillonella was isolated from the saliva of a child. In the present study, the characterization of strain S12025-13^T, is described with the comparison to established species of the genus Veillonella and a novel oral species of the genus Veillonella is proposed.</p><p><strong>Methods: </strong>Strain S12025-13^T was conclusively identified using phenotypic, phylogenetic, genomic and biochemical approach. In phylogenetic analysis, multi-locus species trees (MLST) analysis was also conducted in this study.</p><p><strong>Results: </strong>The strain S12025-13^T showed typical phenotypic characteristics of members of the genus Veillonella. Under anaerobic conditions, the strain produced acetic acid and propionic acid as metabolic end products in a trypticase-yeast extract-heamin medium containing 1 % (w/v) glucose, 1 % (w/v) fructose and 1 % (v/v) sodium lactate. Sequencing of their 16S rRNA genes confirmed that it belongs to the genus Veillonella. Comparative analysis of the 16S rRNA, dnaK and rpoB gene sequences indicated that phylogenetically the strain comprised a distinct novel branch within the genus Veillonella. The novel strain showed 99.77, 97.29, and 99.02 % similarity to partial 16S rRNA, dnaK and rpoB gene sequencing, respectively, to the type strains of the most closely related species. In MLST (selected 79 genes) analysis, strain S12025-13^T formed a distinct taxon with robust bootstrap support (100 %) within the genus Veillonella. Furthermore, strain S12025-13^T shared the highest average nucleotide identity (ANI) value (95.97 %) with the type strain of the most closely related species, \"Veillonella faecalis\" which is not validly published under the International Code of Nomenclature of Prokaryotes (ICNP). Likewise, strain S12025-13^T showed the highest digital DNA-DNA hybridization (dDDH) value (65.00 %) with the type strain of \"V. faecalis\". In addition, strain S12025-13^T formed a distinct branch in this clade with a bootstrap value of 72 % with Veillonella nakazawae and 51 % with \"V. faecalis\" related species of the genus Veillonella in the genome based phylogenetic tree.</p><p><strong>Conclusion: </strong>The novel strain could be discriminated from previously reported species of the genus Veillonella based on partial dnaK gene sequencing, MLST analysis, ANI and dDDH values, and genome-based phylogeny. Based on these observations, this strain represents a novel species, for which the name Veillonella orientalis sp., nov. is proposed. The type strain is S12025-13^T (= JCM 33967^T = CCUG 74596^T).</p>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":" ","pages":"102921"},"PeriodicalIF":2.5,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142708881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of clinical characteristics, risk factors, and outcomes of patients infected with Bacteroides fragilis group at a tertiary care hospital in central China between 2017 and March 2024","authors":"Yongmei Li ,&nbsp;Jing Zhao ,&nbsp;Qiong Ma ,&nbsp;Junhong Xu ,&nbsp;Youhua Yuan ,&nbsp;Qi Zhang ,&nbsp;Wenjuan Yan ,&nbsp;Wenbo Xu ,&nbsp;Zhiyu Yang ,&nbsp;Gang Li ,&nbsp;Lan Gao ,&nbsp;Baoya Wang","doi":"10.1016/j.anaerobe.2024.102922","DOIUrl":"10.1016/j.anaerobe.2024.102922","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aimed to explore the epidemiology, clinical presentation, and risk factors for treatment failure in patients infected with various <em>Bacteroides fragilis</em> group (BFG) species.</div></div><div><h3>Methods</h3><div>We analyzed clinical and laboratory data from 178 patients infected with BFG who were admitted to our hospital between 2017 and 2024. We analyzed categorical data using Chi-square and Fisher exact tests, continuous variables using Student's <em>t</em>-tests or Mann–Whitney <em>U</em>-tests, and risk factors using multivariate logistic regression and Pearson's chi-squared tests.</div></div><div><h3>Results</h3><div><em>Bacteroides fragilis</em> (69.7 %) and <em>Bacteroides thetaiotaomicron</em> (16.9 %) were the most prevalent species among 178 BFG isolates. Most patients had polymicrobial infections, with the following pathogens isolated from concurrent samples: <em>Escherichia coli</em>, <em>Klebsiella</em> spp., and <em>Enterococcus</em> spp. Intra-abdominal sites were most frequently infected with BFG. Patients aged &gt;50 years and those who had intravascular catheters were infected with more other <em>Bacteroides</em> species than <em>B. fragilis</em>. Admission to an ICU, respiratory, renal and cardiovascular diseases, and chest drainage were associated more often with <em>B. thetaiotaomicron</em> than with <em>B. fragilis</em>. Gastrointestinal diseases, tracheal intubation, and ICU admission were associated more frequently with treatment failure in patients infected with <em>B. fragilis</em> whereas solid cancers, renal disease, multiple organ dysfunction syndrome, and tracheal intubation were more likely to be associated with treatment failure in patients infected with other <em>Bacteroides</em> spp.</div></div><div><h3>Conclusions</h3><div>The most prevalent BFG species in the patients were <em>B</em>. <em>fragilis</em> and <em>B</em>. <em>thetaiotaomicron</em>. The demographic characteristics of the patients, underlying diseases, and risk factors for poor clinical outcomes clearly differed among species.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"91 ","pages":"Article 102922"},"PeriodicalIF":2.5,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142708878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}