Anaerobe最新文献

{"title":"Description of three new Leptotrichia species isolated from dental biofilm: Leptotrichia rugosa sp. nov., Leptotrichia mesophila sp. nov. and Leptotrichia alba sp. nov.","authors":"Xia Wang, Shi-Yan Jiao, Jie Wang, Ran-Ran Wu, Tong-Tong Zhang, Chun-Miao Wang, Xiao-Jun Li","doi":"10.1016/j.anaerobe.2025.102948","DOIUrl":"https://doi.org/10.1016/j.anaerobe.2025.102948","url":null,"abstract":"<p><p>Three bacterial strains, namely HSP-334^T, HSP-342^T and HSP-536^T, were isolated from human oral dental biofilm. These strains were identified as Gram-stain-negative, straight or slightly curved anaerobes. Based on 16S rRNA genes analysis, strain HSP-334^T exhibited the closest identity to Leptotrichia shahii LB37^T (92.25%). Strain HSP-342^T demonstrated the highest similarity to Leptotrichia hongkongensis HKU24^T (98.03%), while strain HSP-536^T displayed the greatest resemblance to Leptotrichia buccalis DSM 1135^T (97.77%). Notably, the maximum sequence similarity among the three isolates ranged from 91.56% to 94.12%. All the phylogenies showed that strains HSP-334^T, HSP-342^T, HSP-536^T, all members of genus Leptotrichia and Pseudoleptotrichia goodfellowii JCM 16774^T were clustered in one subclade within the family Leptotrichiaceae. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values calculated between these three strains and their phylogenetically related species were determined to be lower than the established species delineation threshold values. The major cellular fatty acids detected in these novel strains were C_16:0 and C_18:1ω7c. Strains HSP-334^T, HSP-342^T and HSP-536^T could be distinguished from each other by several phenotypic characteristics. Based on the comprehensive polyphasic taxonomic characterizations conducted, strains HSP-334^T, HSP-342^T and HSP-536^T represent three novel species of the genus Leptotrichia, for which the name Leptotrichia rugosa sp. nov. (type strain HSP-334^T = JCM 36566^T = CGMCC 1.18095^T = MCCC 1K09354^T), Leptotrichia mesophila sp. nov. (type strain HSP-342^T = JCM 36567^T = CGMCC 1.18052^T = MCCC 1K09338^T) and Leptotrichia alba sp. nov. (type strain HSP-536^T = JCM 36662^T = CGMCC 1.18096^T = MCCC 1K09339^T) are proposed.</p>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":" ","pages":"102948"},"PeriodicalIF":2.5,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143522351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clostridioides difficile in raw mechanically separated poultry meat and pasteurized product made from contaminated meat.","authors":"Majda Biasizzo, Urška Henigman, Jana Avberšek, Urška Jamnikar-Ciglenečki, Stanka Vadnjal","doi":"10.1016/j.anaerobe.2025.102946","DOIUrl":"https://doi.org/10.1016/j.anaerobe.2025.102946","url":null,"abstract":"<p><strong>Objectives: </strong>Clostridioides difficile (C. difficile) is an important foodborne pathogen found in a wide range of products. This study investigated the occurrence of C. difficile in mechanically separated chicken and turkey meat (MSM) and in pasteurized products made from contaminated MSM.</p><p><strong>Methods: </strong>The presence of C. difficile was analyzed in 56 MSM samples (32 from turkey and 24 from chicken) and in six pasteurized meat products made from raw meats previously identified as C. difficile-positive. After enrichment, detection was performed by real-time PCR, and isolation by bacterial cultivation. The isolated strains were then characterized by PCR-ribotyping, toxinotyping, and whole-genome sequencing (WGS).</p><p><strong>Results: </strong>C. difficile was detected in 16.1 % of MSM samples via real-time PCR, with a 10.7 % isolation rate. One pasteurized product also tested positive. The six isolates obtained displayed diverse PCR-ribotypes, five of which were toxigenic. Notably, the PCR-ribotypes and sequence types in the pasteurized product differed from those identified in the raw meat used for its production.</p><p><strong>Conclusions: </strong>The presence of C. difficile in raw and subsequently pasteurized meat product indicates that the pathogen can survive the pasteurization process and may be present in such products.</p>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":" ","pages":"102946"},"PeriodicalIF":2.5,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143514189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cutibacterium avidum: A virulent pathogen in esthetic surgery infection, a case series.","authors":"Ramírez-Sánchez Isabel Cristina, Posada-Rios Diego","doi":"10.1016/j.anaerobe.2025.102944","DOIUrl":"https://doi.org/10.1016/j.anaerobe.2025.102944","url":null,"abstract":"<p><p>Cutibacterium avidum is a member of the skin microbiota whose composition changes with age. Recently, it has been implicated in infections associated with implants and other medical devices, and it is now recognized as an etiological agent of surgical site infections. We present six cases of surgical site infections following aesthetic surgery: three cases linked to gluteal implants, one to gluteoplasty without implants, one to liposuction and one to abdominoplasty. Previously, C. avidum was considered a contaminant; however, recent findings indicate virulence factors and pathogenic behavior, so now is regarded as a potential causative agent of infection.</p>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":" ","pages":"102944"},"PeriodicalIF":2.5,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143514241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coculture systems to study interactions between anaerobic bacteria and intestinal epithelium","authors":"Lyudmila Boyanova,&nbsp;Raina Gergova,&nbsp;Rumyana Markovska","doi":"10.1016/j.anaerobe.2025.102949","DOIUrl":"10.1016/j.anaerobe.2025.102949","url":null,"abstract":"<div><div>Coculture systems (CCSs) are experimental tools used to study the interactions of anaerobic bacteria among themselves and the gut epithelial cells under conditions simulating the human gut, unlike those in animal models. Although the studies on animal models are useful in determining the relationship between the causative agents of infections and human infections, they have disadvantages, such as ethical issues, in addition to the differences in the microbiota of the animal and humans. Therefore, the results obtained using animal models cannot be directly extrapolated to humans. CCSs can more completely reflect <em>in vivo</em> gut homeostasis and contribute to better understanding of the interplay between the intestinal cells and anaerobes, prevalent among the gut bacteria. Moreover, they provide new insights on the pathogenesis of infections and aid in assessing the usefulness of new probiotics and antibacterials. Therefore, CCSs, including the gut-on-a-chip models, can significantly improve microbiota-based therapy. Moreover, they can also be used to detect microbiota-derived metabolites such as those with mutagenic properties. The aim of this review was to explore selected CCS models of anaerobes with intestinal epithelium and their application in investigating intestinal homeostasis. The focus was to highlight the application of different CCSs and important data obtained from their implementation.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"92 ","pages":"Article 102949"},"PeriodicalIF":2.5,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143514263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Veillonella orientalis sp. nov., an anaerobic Gram-stain-negaitve coccus isolated from saliva of a Thai child","authors":"Izumi Mashima-Usami ,&nbsp;Citra F. Theodorea ,&nbsp;Kiyoshi Murata ,&nbsp;Boonyanit Thaweboon ,&nbsp;Sroisiri Thaweboon ,&nbsp;Futoshi Nakazawa","doi":"10.1016/j.anaerobe.2024.102921","DOIUrl":"10.1016/j.anaerobe.2024.102921","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Objective&lt;/h3&gt;&lt;div&gt;The genus &lt;em&gt;Veillonella&lt;/em&gt; is one of the major and important constitutions of the oral microbiome.&lt;/div&gt;&lt;div&gt;A novel anaerobic, Gram-negative coccus belonging to the genus &lt;em&gt;Veillonella&lt;/em&gt; was isolated from the saliva of a child. In the present study, the characterization of strain S12025-13&lt;sup&gt;T&lt;/sup&gt;, is described with the comparison to established species of the genus &lt;em&gt;Veillonella&lt;/em&gt; and a novel oral species of the genus &lt;em&gt;Veillonella&lt;/em&gt; is proposed.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;div&gt;Strain S12025-13&lt;sup&gt;T&lt;/sup&gt; was conclusively identified using phenotypic, phylogenetic, genomic and biochemical approach. In phylogenetic analysis, multi-locus species trees (MLST) analysis was also conducted in this study.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;The strain S12025-13&lt;sup&gt;T&lt;/sup&gt; showed typical phenotypic characteristics of members of the genus &lt;em&gt;Veillonella&lt;/em&gt;. Under anaerobic conditions, the strain produced acetic acid and propionic acid as metabolic end products in a trypticase-yeast extract-heamin medium containing 1 % (w/v) glucose, 1 % (w/v) fructose and 1 % (v/v) sodium lactate. Sequencing of their 16S rRNA genes confirmed that it belongs to the genus &lt;em&gt;Veillonella&lt;/em&gt;. Comparative analysis of the 16S rRNA, &lt;em&gt;dnaK&lt;/em&gt; and &lt;em&gt;rpoB&lt;/em&gt; gene sequences indicated that phylogenetically the strain comprised a distinct novel branch within the genus &lt;em&gt;Veillonella&lt;/em&gt;. The novel strain showed 99.77, 97.29, and 99.02 % similarity to partial 16S rRNA, &lt;em&gt;dnaK&lt;/em&gt; and &lt;em&gt;rpoB&lt;/em&gt; gene sequencing, respectively, to the type strains of the most closely related species. In MLST (selected 79 genes) analysis, strain S12025-13&lt;sup&gt;T&lt;/sup&gt; formed a distinct taxon with robust bootstrap support (100 %) within the genus &lt;em&gt;Veillonella&lt;/em&gt;. Furthermore, strain S12025-13&lt;sup&gt;T&lt;/sup&gt; shared the highest average nucleotide identity (ANI) value (95.97 %) with the type strain of the most closely related species, “&lt;em&gt;Veillonella faecalis&lt;/em&gt;” which is not validly published under the International Code of Nomenclature of Prokaryotes (ICNP). Likewise, strain S12025-13&lt;sup&gt;T&lt;/sup&gt; showed the highest digital DNA-DNA hybridization (dDDH) value (65.00 %) with the type strain of “&lt;em&gt;V. faecalis&lt;/em&gt;”. In addition, strain S12025-13&lt;sup&gt;T&lt;/sup&gt; formed a distinct branch in this clade with a bootstrap value of 72 % with &lt;em&gt;Veillonella nakazawae&lt;/em&gt; and 51 % with “&lt;em&gt;V. faecalis&lt;/em&gt;” related species of the genus &lt;em&gt;Veillonella&lt;/em&gt; in the genome based phylogenetic tree.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusion&lt;/h3&gt;&lt;div&gt;The novel strain could be discriminated from previously reported species of the genus &lt;em&gt;Veillonella&lt;/em&gt; based on partial &lt;em&gt;dnaK&lt;/em&gt; gene sequencing, MLST analysis, ANI and dDDH values, and genome-based phylogeny. Based on these observations, this strain represents a novel species, for which the name &lt;em&gt;Veillonella orientalis&lt;/em&gt; sp., nov. is proposed. The type strain is S12025-13&lt;sup&gt;T&lt;/sup&gt; (= JC","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"91 ","pages":"Article 102921"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142708881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Presence of Clostridioides difficile on spinach, carrots, cheese and milk in Turkey","authors":"Gizem Taylan Yalçın ,&nbsp;Melike Nur Tosun Demir ,&nbsp;Gizem Korkmazer ,&nbsp;Alper Akçalı ,&nbsp;Nükhet Nilüfer Demirel Zorba","doi":"10.1016/j.anaerobe.2024.102933","DOIUrl":"10.1016/j.anaerobe.2024.102933","url":null,"abstract":"<div><h3>Introduction</h3><div>The presence of <em>Clostridioides difficile</em> in water, soil, fertilizers, and animal feces suggests the potential existence of <em>C. difficile</em> in foods that come into contact with these sources or become contaminated through indirect means.</div></div><div><h3>Material &amp; method</h3><div>A total of 431 samples, consisting of spinach and carrots and raw milk and cheese obtained from cows, goats, buffalo, and sheep, were examined for the presence of <em>C. difficile</em>. Isolates were identified by real-time PCR, ribotyped, and their toxin profiles were determined. Antibiotic susceptibility to vancomycin, clindamycin, and metronidazole was evaluated using the E-test.</div></div><div><h3>Results</h3><div><em>C. difficile</em> was detected in 3.27 % (4/122) of spinach, 1.85 % (2/108) of carrots, and 2.19 % (2/91) of milk samples. No <em>C. difficile</em> was detected in the cheeses (n = 110). All isolates were obtained from different fields/farms. Only one isolate (from spinach) carried the <em>tcdA</em> and <em>tcdB</em> toxin genes. Six different PCR ribotypes were detected, with two (001, 060) being identified. All isolates were sensitive to vancomycin, clindamycin, and metronidazole.</div></div><div><h3>Conclusion</h3><div>The prevalence of <em>C. difficile</em> in spinach, carrot, and milk samples from selected regions was low, and nontoxigenic strains were prevalent. Despite the low prevalence, the detection of <em>C. difficile</em> in these foods highlights the potential risk of foodborne transmission of this pathogen and underscores the need for monitoring and control strategies to ensure food safety.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"91 ","pages":"Article 102933"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clostridioides difficile infection and testing rates in South Africa: A multicentre study, 2017–2020","authors":"Trusha Nana ,&nbsp;Praksha Ramjathan ,&nbsp;Khine Swe-Swe Han ,&nbsp;Kessendri Reddy","doi":"10.1016/j.anaerobe.2024.102937","DOIUrl":"10.1016/j.anaerobe.2024.102937","url":null,"abstract":"<div><h3>Objectives</h3><div>To describe <em>Clostridioides difficile</em> infection (CDI) rates and testing practices, at three tertiary/quaternary hospitals in South Africa (SA) for the period 2017 to 2020.</div></div><div><h3>Methods</h3><div>A retrospective laboratory record review of all <em>C. difficile</em> testing at the Charlotte Maxeke Johannesburg Academic Hospital (CMJAH), Tygerberg Hospital (TBH) and Inkosi Albert Luthuli Central Academic Hospital (IALCH) was performed. Clinical records of patients with rCDI were reviewed to determine recurrent CDI (rCDI) rates.</div></div><div><h3>Results</h3><div>The median primary CDI rates per 10 000 patient-days (PD) were 5.3 at CMJAH, 1.8 at TBH, and 0.3 at IALCH. In 2020, all hospitals reported an increase in primary CDI rates compared to 2019. The median testing rates per 10 000 PD were 39 at CMJAH, 14 at TBH, and 4 at IALCH. The median age of patients with primary CDI was 33 years (IQR: 22–45 years). The rCDI rates ranged from 2 to 5 per 100 incident episodes.</div></div><div><h3>Conclusion</h3><div>Significant variations in CDI and testing rates were observed across the three hospitals. An increase in CDI rates was noted at all centres during the 2020 SARS-CoV-2 outbreak. Advanced age was not prevalent in the cohort, and rCDI rates were relatively low. These findings highlight the need for systematic surveillance of healthcare-onset CDI across SA hospitals.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"91 ","pages":"Article 102937"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142875924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fusobacterium necrophorum septic arthritis of the hip: A case-report and literature review","authors":"Giacomo Franceschi ,&nbsp;Mattia Marchi ,&nbsp;Francesco Zambianchi ,&nbsp;Marianna Meschiari ,&nbsp;Cristina Mussini ,&nbsp;Andrea Bedini","doi":"10.1016/j.anaerobe.2024.102934","DOIUrl":"10.1016/j.anaerobe.2024.102934","url":null,"abstract":"<div><h3>Introduction</h3><div><em>Fusobacterium necrophorum</em> is a rare but significant cause of septic arthritis, typically following oropharyngeal infections in adolescents. This anaerobic pathogen, commonly associated with Lemierre’s syndrome, can lead to joint infections, posing risks for severe morbidity if diagnosis and treatment are delayed. Awareness and timely intervention are essential for preventing long-term joint damage.</div></div><div><h3>Case report</h3><div>We report the case of a 19-year-old woman who developed high fever and acute right hip pain one week after a sore throat. Imaging revealed septic arthritis, with <em>F. necrophorum</em> identified in both blood and synovial fluid cultures. She received intravenous piperacillin/tazobactam, followed by outpatient parenteral therapy through an elastomeric pump, achieving full recovery. This case adds to the 42 cases documented in our literature review, reinforcing the need for prompt antimicrobial therapy.</div></div><div><h3>Conclusion</h3><div><em>Fusobacterium</em>-induced septic arthritis, though uncommon, should be considered in young patients presenting with joint infections post-pharyngitis. Early diagnosis and targeted antimicrobial therapy, particularly with β-lactamase inhibitors, are critical for effective management and preventing joint sequelae.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"91 ","pages":"Article 102934"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142817076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clostridioides difficile hypervirulent strain ST1 isolated from clinical stool specimens obtained from three Provinces in South Africa","authors":"Hlambani Shirinda ,&nbsp;Anthony M. Smith ,&nbsp;Ben Prinsloo ,&nbsp;Marleen M. Kock ,&nbsp;Mishalan Moodley ,&nbsp;Mohamed Said ,&nbsp;Marthie M. Ehlers","doi":"10.1016/j.anaerobe.2024.102926","DOIUrl":"10.1016/j.anaerobe.2024.102926","url":null,"abstract":"<div><h3>Objectives</h3><div><em>Clostridioides difficile</em> infection is a serious healthcare-associated infection linked to antimicrobial use. The severity of the disease can be associated with hypervirulent ribotypes such as RT027. The study aimed to investigate the molecular epidemiology and genomic characteristics of <em>C. difficile</em> isolates from private and public healthcare settings in South Africa.</div></div><div><h3>Methods</h3><div>One hundred clinical stool specimens were cultured on cycloserine-cefoxitin-fructose agar. Conventional multiplex polymerase chain reaction (M-PCR) assays were conducted for isolate identification and detection of toxin genes. Genomic characteristics of the isolates were determined using whole genome sequencing (WGS) and data was analysed using pubMLST, EnteroBase, Pathogenwatch and CARD.</div></div><div><h3>Results</h3><div>One hundred clinically presumptive <em>C. difficile</em> positive stool specimens were collected, of which 62 % (62/100) were confirmed as <em>C. difficile</em> by M-PCR assay. Among the 62 identified <em>C. difficile</em> isolates, 97 % (60/62) were toxigenic, with the most dominant toxin profile being <em>A</em> ^<em>+</em> <em>B</em> ^<em>+</em> <em>CDT</em> ^<em>+</em> according to the M-PCR assay. The results showed that 93 % (40/43) of the WGS analysed <em>C. difficile</em> strains clustered into clades 1 to 5. These 40 strains were categorized into 16 sequence types (STs), with ST1 (clade 2) being the most prevalent, representing 45 % (18/40), this strain is an RT027-associated strain previously epidemic hypervirulent strain. One major cluster (n = 18) comprising ST1 strains was identified in Gauteng Province and all the isolates associated with this cluster showed the same resistome (antimicrobial resistance genes and mutations: <em>CDD-1</em>, <em>aac (6′)-Ie-aph (2″)-Ia, PnimB</em>^<em>G</em> and <em>Thr82Ile</em>). The study also identified one strain as ST11, this strain is well known for its zoonotic potential, and two strains were identified as ST37 known as an epidemic strain. Strains from public healthcare settings exhibited genetic similarity, while those from private settings showed greater genetic diversity.</div></div><div><h3>Conclusion</h3><div>The study reported, for the first time, hypervirulent strains ST1 in Africa and ST11 in South Africa, with a minimum spanning tree indicating an ongoing ST1 outbreak.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"91 ","pages":"Article 102926"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Description of Anaerococcus kampingiae sp. nov., Anaerococcus groningensis sp. nov., Anaerococcus martiniensis sp. nov., and Anaerococcus cruorum sp. nov., isolated from human clinical specimens","authors":"K.E. Boiten,&nbsp;J. Meijer,&nbsp;E.M. van Wezel,&nbsp;A.C.M. Veloo","doi":"10.1016/j.anaerobe.2024.102935","DOIUrl":"10.1016/j.anaerobe.2024.102935","url":null,"abstract":"<div><h3>Objectives</h3><div>To improve the identification of anaerobic bacteria, the identity of clinical isolates which could not be identified using MALDI-TOF MS was assessed using whole genome sequencing (WGS) and in-house made main spectral profiles (MSPs) were created. Four novel <em>Anaerococcus</em> species, each represented by at least two isolates, were encountered.</div></div><div><h3>Methods</h3><div>The novelty of the isolates was confirmed by comparing the 16S rRNA gene sequences and the WGS with their closest relatives. Phylogenetic and phylogenomic relationships were determined using MEGA X and DSMZ TYGS. Biochemical features were determined and the clustering of the created MSPs was calculated. Possible clinical relevance was assessed.</div></div><div><h3>Results</h3><div>The novelty of the four different species was confirmed by the ANI value, and phylogenetic/phylogenomic clustering. Three of these species shared the same biochemical features, while one showed a different pattern. Only this latter species can be differentiated from other <em>Anaerococcus</em> spp. Remarkebly, six of the ten isolates were obtained from a positive blood culture, of which in five cases the bacterium was the only species encountered.</div></div><div><h3>Conclusions</h3><div>We propose to name these novel species: <em>Anaerococcus kampingiae</em> (ENR0874^T = DSM 117234^T, CCUG 77487^T (accession numbers PP192775/JBGMEF000000000)), <em>Anaerococcus groningensis</em> (ENR1011^T = DSM 117232^T, CCUG 77488^T (accession numbers PP192777/JBGMEG000000000)), <em>Anaerococcus martiniensis</em> (ENR0831^T = DSM 117233^T, CCUG 77486^T (accession numbers PP192776/JBGMEI000000000)), and <em>Anaerococcus cruorum</em> (ENR1039^T = DSM 117235^T, CCUG 77489^T (accession numbers PP192778/JBGMEH000000000)).</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"91 ","pages":"Article 102935"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142817073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}