Anaerobe最新文献

{"title":"High prevalence of Clostridioides difficile in retail garden products from two Australian states.","authors":"Jessica M Chisholm, Charlotte Bell, Molly Lattin, Su Chen Lim, Simon A Reid, Linda A Selvey, Thomas V Riley","doi":"10.1016/j.anaerobe.2025.102987","DOIUrl":"https://doi.org/10.1016/j.anaerobe.2025.102987","url":null,"abstract":"<p><strong>Background: </strong>Despite historically being considered a healthcare-associated pathogen, rates of Clostridioides difficile infection in the community have risen greatly in recent decades. As a pathogen of One Health importance, sources of C. difficile in the community have been sought to understand and control community-associated C. difficile infection transmission.</p><p><strong>Objectives: </strong>This study aimed to investigate the prevalence and molecular characteristics of C. difficile isolated from retail garden products in two Australian states.</p><p><strong>Methods: </strong>Soil conditioners, soil mixes and turf samples were collected from commercial garden product suppliers in Western Australia and Queensland from May 2023 to January 2024. Following enrichment culture, PCR ribotyping and toxin gene profiling were performed on C. difficile isolates.</p><p><strong>Results: </strong>C. difficile was recovered from 257 of 479 (53.7%) retail garden products in this study. Prevalence was highest in turf (67.1%, 53/79), followed by soil mixes (57.1%, 148/259) and soil conditioners (39.7%, 56/141). Of the 113 distinct ribotypes identified, the most common were 014/020 and QX 686. Toxigenic ribotypes associated with both human infection and production animals in Australia, including 014/020, 056 and 087, were recovered.</p><p><strong>Conclusion: </strong>A high prevalence of C. difficile was found in garden products sold to consumers, including toxigenic strains associated with C. difficile infection in humans and animals. More discriminatory characterisation of isolates is required to determine if retail garden products contribute to the burden of community-associated C. difficile infection in Australia.</p>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":" ","pages":"102987"},"PeriodicalIF":2.6,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144726869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxigenic, atypical strains of Clostridioides difficile isolated from soils of Western Australian public parks","authors":"Paige McGraw ,&nbsp;Su-Chen Lim ,&nbsp;Deirdre A. Collins ,&nbsp;Thomas V. Riley","doi":"10.1016/j.anaerobe.2025.102986","DOIUrl":"10.1016/j.anaerobe.2025.102986","url":null,"abstract":"<div><div>Public park soils in Perth, Western Australia were investigated for <em>Clostridioides difficile. C. difficile</em> was found at a high prevalence of 73 %. Over 19 % of the <em>C. difficile</em> isolates were toxigenic, including clinically relevant <em>C. difficile</em> ribotypes 014/020 and 106. Additionally, an esculin-hydrolysis negative, A⁺B⁻CDT^- isolate was recovered.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"94 ","pages":"Article 102986"},"PeriodicalIF":2.6,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144681904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization of Clostridioides difficile isolated from older adults during prospective population-based active surveillance at nine hospitals in Tokyo, Japan, 2018-2020.","authors":"Zhenghui Li, Ashlesh K Murthy, C Hal Jones, Kwok Lee, Urvi Rajyaguru, Deepika Athinarayanan, Lubomira Andrew, Richard V Goering, Shuhei Ito, Elisa Gonzalez, Pingping Zhang, Paul A Liberator, Warren V Kalina, Michael W Pride, Frederick J Angulo, Jennifer C Moïsi, Kazuhiro Tateda","doi":"10.1016/j.anaerobe.2025.102985","DOIUrl":"https://doi.org/10.1016/j.anaerobe.2025.102985","url":null,"abstract":"<p><strong>Background: </strong>To characterize Clostridioides difficile isolates identified during a prospective multi-hospital population-based study of C. difficile infection (CDI).</p><p><strong>Methods: </strong>Between December 2018 and March 2020, inpatients ≥50 years of age with new-onset diarrhea in nine Tokyo hospitals were investigated for CDI.. Stool specimens were screened by C. DIFF QUIK CHEK COMPLETE® and glutamate dehydrogenase (GDH) positive stools tested by Xpert® C. difficile/Epi PCR assay. PCR positive stools were tested by cytotoxicity neutralization assay to determine presence of functional toxin. GDH positive stools were also anaerobically cultured and whole genome sequences of C. difficile isolates were acquired.</p><p><strong>Results: </strong>Toxigenic C. difficile were isolated from 64 patients with CDI; 22 were RT018/356, 9 were RT369, 4 each were RT106 and RT002, 15 were other RTs, and 10 had an unknown RT. Four isolates were positive for binary toxin: one isolate each of RT027, RT078/126, RT080, and one unknown. In terms of the pathogenicity locus profile, 55 were tcdA+/tcdB+ and 9 were tcdA-/tcdB+. In terms of ST, 23 were ST17, nine were ST81, 31 were other STs, and one had a novel ST.</p><p><strong>Conclusions: </strong>Consistent with prior studies, C. difficile isolates from patients with CDI represented diverse ribotypes with approximately one third of isolates RT018/356. Of note, one isolate each of RT027 and RT078/126 were identified, indicating these hypervirulent strains are present in hospitals in Japan. Public health interventions are needed to reduce the CDI burden in Japan.</p>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":" ","pages":"102985"},"PeriodicalIF":2.5,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144666880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on acute exposure of polyethylene terephthalate microplastics on the performance of anammox granular sludge","authors":"Yuliang Zhao,&nbsp;Haijuan Qin,&nbsp;Yuhua Lyu,&nbsp;Song Yan,&nbsp;Huali Yu","doi":"10.1016/j.anaerobe.2025.102984","DOIUrl":"10.1016/j.anaerobe.2025.102984","url":null,"abstract":"<div><h3>Background</h3><div>Anaerobic ammonia oxidation is a new and efficient biological denitrification process, which has been used in practice. Research on the effect of microplastics (MPs) on anaerobic ammonia oxidation (anammox) process is limited.</div></div><div><h3>Topic</h3><div>In this study, the effects of short-term exposure of polyethylene terephthalate (PET) with different particle sizes for 12 h on anammox granular sludge (AnGS) and the metabolic mechanism of anaerobic ammonia oxidizing bacteria (AnAOB) were studied.</div></div><div><h3>Results</h3><div>The results showed that the acute exposure of anammox reactor to PET-MPs could promote the denitrification rate of the reactor. The enzyme activity of hydrazine dehydrogenase (HDH), the key enzyme of anammox reaction, was increased under acute exposure of different particle sizes of PET-MPs. PET-MPs with larger particle size had a stronger promoting effect on the treatment efficiency and HDH activity of the reactor. PET-MPs could stimulate AnAOB to secrete a large amount of extracellular polymeric substances (EPS). Metabonomic analysis showed that after the addition of PET-MPs, the abundance of 3-methoxytyramine was down-regulated, and the abundance of L-serine decreased. In the 13 μm PET-MPs group, the abundance of L-asparagine was up-regulated, changes in these metabolites affected various amino acid metabolism, proteolytic enzymes, and AnAOB biosynthesis.</div></div><div><h3>Implication</h3><div>This study provides valuable insights into the response characteristics and mechanism of the anammox process exposed to MPs, which may be helpful for the application of MPs in wastewater treatment.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"94 ","pages":"Article 102984"},"PeriodicalIF":2.5,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144599208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clostridioides difficile in Australian dairy farms","authors":"Su-Chen Lim ,&nbsp;Jessica Chisholm ,&nbsp;Deirdre A. Collins ,&nbsp;Molly Lattin ,&nbsp;Charlotte Bell ,&nbsp;Linda Selvey ,&nbsp;Simon Reid ,&nbsp;Thomas V. Riley","doi":"10.1016/j.anaerobe.2025.102983","DOIUrl":"10.1016/j.anaerobe.2025.102983","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aimed to investigate the prevalence and diversity of <em>C. difficile</em> in Australian dairy farms.</div></div><div><h3>Methods</h3><div>A total of 320 samples, including faeces, rectal swabs, effluent, soil and water, were collected from seven dairy farms across two Australian states.</div></div><div><h3>Results</h3><div><em>C. difficile</em> was present in all seven farms (100 %) and 44.4 % of the samples tested, with the highest prevalence found in soil (94.3 %), effluent (73.7 %) and faeces (60.4 %). Forty-four distinct ribotypes were identified among 174 isolates, of which 17 have been found previously in humans. <em>C. difficile</em> ribotype (RT) 127 (A + B + CDT+) was the predominant strain, comprising 42.5 % of isolates. The findings also indicated that the environment of Australian dairy farms is persistently contaminated with <em>C. difficile</em>, especially RT 127 and may represent a potential source for zoonotic transmission.</div></div><div><h3>Conclusions</h3><div><em>C. difficile</em> may be widespread in Australian dairy farms, with notably high prevalence in both animals and environmental samples.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"94 ","pages":"Article 102983"},"PeriodicalIF":2.5,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144607222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clostridioides (Clostridium) difficile in children and adolescents in the community in Cambodia","authors":"Lengsea Eng ,&nbsp;Paul Turner ,&nbsp;Kefyalew Addis Alene ,&nbsp;Deirdre A. Collins ,&nbsp;Su-Chen Lim ,&nbsp;Pisey Tan ,&nbsp;Sona Soeng ,&nbsp;Dylorng Hun ,&nbsp;Sotera Yohn ,&nbsp;Sarim Vong ,&nbsp;Archie C.A. Clements ,&nbsp;Thomas V. Riley","doi":"10.1016/j.anaerobe.2025.102982","DOIUrl":"10.1016/j.anaerobe.2025.102982","url":null,"abstract":"<div><h3>Background</h3><div><em>Clostridioides (Clostridium) difficile</em> transmission between community and healthcare settings has been increasingly reported. We aimed to identify the prevalence and molecular epidemiology of <em>C. difficile</em> colonising adolescents and non-hospitalised children in Cambodia.</div></div><div><h3>Methods</h3><div>Stool samples were collected from 266 students at the University of Health Sciences (UHS) in Phnom Penh, between July and August 2022, and 246 children ≥1 year old visiting the outpatient department (OPD) at Angkor Hospital for Children in Siem Reap, between January and August 2022. <em>C. difficile</em> culture, toxin gene detection and PCR ribotyping were performed.</div></div><div><h3>Results</h3><div>Overall, <em>C. difficile</em> was recovered from 07/266 specimens (2.6 %) from UHS students and 59/246 specimens (23.9 %) from OPD children. The overall prevalence of <em>C. difficile</em> in children peaked in April, and of toxigenic strains peaked in August. Children with loose stools were less likely to be colonised by <em>C. difficile</em> (COR = 0.05, 95 %CI: 0.008–0.32) than children with normal stools. UHS students were colonised only by toxigenic <em>C. difficile.</em> Of the 66 isolates, 36 % (24/66) were toxigenic: <em>C. difficile</em> ribotype (RT) 017 was the most predominant, followed by RTs 012, 046, 056, QX709, 001, 014/020 and QX710. Non-toxigenic strains accounted for 35 RTs including 32 novel RTs that had not been isolated previously.</div></div><div><h3>Conclusions</h3><div>The findings of only toxigenic strains in adolescents and the high prevalence of <em>C. difficile</em> in OPD children suggest exposure to <em>C. difficile</em> within the community; thus, appropriate interventions may be needed. Genotypic identification of diverse <em>C. difficile</em> is important for molecular epidemiological purposes.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"94 ","pages":"Article 102982"},"PeriodicalIF":2.5,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144564307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The first case report of an intraosseous hemangioma complicated by Prevotella bivia infection","authors":"Hrisi Bahar Tokman ,&nbsp;Muhammed Yusuf Afacan ,&nbsp;Arin Celayir ,&nbsp;Ece Davutluoglu ,&nbsp;Ayse Akgonul ,&nbsp;Ayse Ceylan Kilincarslan ,&nbsp;Edip Tokuc ,&nbsp;Mahmut Kursat Ozsahin ,&nbsp;Huseyin Botanlioglu","doi":"10.1016/j.anaerobe.2025.102981","DOIUrl":"10.1016/j.anaerobe.2025.102981","url":null,"abstract":"<div><div>Intraosseous hemangiomas are rare benign vascular tumors that occur within the bone. <em>Prevotella bivia</em> is an anaerobic, gram-negative rod that is part of the normal microbiota of the gastrointestinal and urogenital tract as well as the oral cavity, but it rarely causes bone infections. This case report presents a patient with a history of dental infection who was initially diagnosed radiologically with non-ossifying fibroma (NOF) in the knee. Histopathology and anaerobic culture identified the lesion as an intraosseous hemangioma infected with <em>P</em>.<em>bivia</em>. The patient was treated with amoxicillin-clavulanic acid and metronidazole. This case report underscores the importance of considering rare pathogens in the differential diagnosis of bone lesions when clinical and radiological findings do not align.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"94 ","pages":"Article 102981"},"PeriodicalIF":2.5,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144526215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenotypic and genomic characterization of Clostridioides difficile strains involved in multiple recurrences","authors":"Anne Lecoutour ,&nbsp;Victoria Mesa ,&nbsp;Jeanne Couturier ,&nbsp;Johanne Delannoy ,&nbsp;Bénédicte Pigneur ,&nbsp;Frédéric Barbut","doi":"10.1016/j.anaerobe.2025.102980","DOIUrl":"10.1016/j.anaerobe.2025.102980","url":null,"abstract":"<div><h3>Background</h3><div>Multiple relapses of <em>Clostridioides difficile</em> infections (CDI) are frequent, impact patients' quality of life, and are challenging to treat. These relapses are either linked to the patient's conditions (immunity, intestinal dysbiosis) or potentially to strain-specific characteristics.</div></div><div><h3>Objectives</h3><div>This study aims to determine the genomic and phenotypic characteristics of <em>C. difficile</em> (CD) strains associated with multiple relapses of CDIs (rCDI).</div></div><div><h3>Material and methods</h3><div>Between 2019 and 2022, 10 CD strains responsible for multiple (≥2) relapses were matched on PCR ribotype to 10 CD control strains isolated from patients with a single episode. Genomic (resistome, virulome, mobile genetic elements) and phenotypic (motility, sporulation, germination, biofilm production, stress resistance) characteristics of relapse and control strains were compared.</div></div><div><h3>Results</h3><div>No significant genomic or phenotypic differences were identified between strains involved in multiple relapses and control strains. Our analyses revealed significant genomic and phenotypic variability among strains.</div></div><div><h3>Conclusion</h3><div>The origin of rCDI does not seem to be directly related to the <em>C. difficile</em> strain, suggesting that these relapses are more likely associated with other factors, such as intestinal dysbiosis or the patient's immune status.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"94 ","pages":"Article 102980"},"PeriodicalIF":2.5,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144511412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring benzene mineralization by anaerobes isolated from denitrifying enrichment cultures","authors":"Samuel C. Eziuzor ,&nbsp;Carsten Vogt","doi":"10.1016/j.anaerobe.2025.102979","DOIUrl":"10.1016/j.anaerobe.2025.102979","url":null,"abstract":"<div><h3>Objectives</h3><div>The study aimed to isolate and characterize benzene-mineralizing anaerobes from a nitrate-reducing community. The goal was to evaluate their potential for benzene degradation under anoxic conditions and to compare composition and metabolic activity in media with and without ammonium.</div></div><div><h3>Methods</h3><div>Two putative isolates (Bz4 and Bz7) were obtained using classical isolation techniques under nitrate-reducing conditions with either acetate or benzene as the sole carbon and energy source. Enrichment media differed by the presence (Bz4) or absence (Bz7) of ammonium. The ability of the isolates to mineralize [¹³C]-labeled acetate and [¹³C₆]-labeled benzene was assessed by monitoring ¹³CO₂ production under anoxic conditions over 184 days. Nitrite production was also measured. Community composition was determined via 16S rRNA gene amplicon sequencing.</div></div><div><h3>Results</h3><div>Both Bz4 and Bz7 consortia mineralized [¹³C]-acetate anoxically, with mineralization rates of 3.3 μM day⁻¹ and 2.7 μM day⁻¹, respectively. Benzene mineralization was observed only in Bz4, which degraded [¹³C₆]-benzene at a rate of 0.298 μM day⁻¹, resulting in ¹³CO₂ production with δ¹³C values reaching 960.2 ± 0.3 ‰. Bz4 also produced nitrite (4.60 ± 0.004 μM), while Bz7 showed no benzene mineralization with a smaller quantity of nitrite production. Microbial community analysis revealed that Bz4 was dominated by <em>Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium</em> (51.4 %), <em>Rhizobiaceae</em> (21.0 %), <em>Pseudomonas</em> (18.2 %), and <em>Nocardioides</em> (8.9 %). In contrast, Bz7 was almost exclusively composed of <em>Simplicispira</em> (96.7 %).</div></div><div><h3>Conclusions</h3><div>The Bz4 consortium demonstrated effective anaerobic benzene mineralization under nitrate-reducing conditions, highlighting its potential for further purification and study in the context of anaerobic benzene biodegradation.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"94 ","pages":"Article 102979"},"PeriodicalIF":2.5,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and characterization of anaerobic bacteria in human semen: A retrospective study","authors":"Marek Kwaśniewski ,&nbsp;Tomasz M. Karpiński","doi":"10.1016/j.anaerobe.2025.102977","DOIUrl":"10.1016/j.anaerobe.2025.102977","url":null,"abstract":"<div><div>This study analyzed retrospectively 291 semen samples, identifying diverse anaerobic bacteria, mainly <em>Prevotella</em> (49 % of all isolates), <em>Veillonella</em>, and <em>Peptoniphilus</em>. Most isolates were susceptible to amoxicillin/clavulanic acid, suggesting its therapeutic potential in male infertility. Resistance to metronidazole was observed in <em>Peptoniphilus, Veillonella</em>, and <em>Propionimicrobium</em>, while <em>Bacteroides</em> exhibited resistance to benzylpenicillin.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"94 ","pages":"Article 102977"},"PeriodicalIF":2.5,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}