艰难梭菌在澳大利亚两个州零售园艺产品中的高流行率。

IF 2.6 3区 生物学 Q3 MICROBIOLOGY
Jessica M Chisholm, Charlotte Bell, Molly Lattin, Su Chen Lim, Simon A Reid, Linda A Selvey, Thomas V Riley
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引用次数: 0

摘要

背景:尽管历史上被认为是一种与卫生保健相关的病原体,但近几十年来,艰难梭菌在社区中的感染率大幅上升。作为一种具有“同一个健康”重要性的病原体,人们一直在寻求社区中艰难梭菌的来源,以了解和控制社区相关的艰难梭菌感染传播。目的:本研究旨在调查澳大利亚两个州零售园艺产品中分离的艰难梭菌的流行率和分子特征。方法:于2023年5月至2024年1月在西澳大利亚州和昆士兰州的商业园艺产品供应商处采集土壤调理剂、土壤混合料和草坪样品。富集培养后,对难辨梭菌分离株进行PCR核糖分型和毒素基因谱分析。结果:从479种零售园艺产品中检出艰难梭菌257种(53.7%)。草皮患病率最高(67.1%,53/79),其次是混合土(57.1%,148/259)和土壤调理剂(39.7%,56/141)。在鉴定出的113种不同的核型中,最常见的是014/020和QX 686。在澳大利亚发现了与人类感染和生产动物相关的产毒核型,包括014/020、056和087。结论:在销售给消费者的园艺产品中发现艰难梭菌的高发率,包括与人类和动物中艰难梭菌感染相关的产毒菌株。需要对分离株进行更具歧视性的特征描述,以确定零售园艺产品是否会导致澳大利亚社区相关艰难梭菌感染的负担。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
High prevalence of Clostridioides difficile in retail garden products from two Australian states.

Background: Despite historically being considered a healthcare-associated pathogen, rates of Clostridioides difficile infection in the community have risen greatly in recent decades. As a pathogen of One Health importance, sources of C. difficile in the community have been sought to understand and control community-associated C. difficile infection transmission.

Objectives: This study aimed to investigate the prevalence and molecular characteristics of C. difficile isolated from retail garden products in two Australian states.

Methods: Soil conditioners, soil mixes and turf samples were collected from commercial garden product suppliers in Western Australia and Queensland from May 2023 to January 2024. Following enrichment culture, PCR ribotyping and toxin gene profiling were performed on C. difficile isolates.

Results: C. difficile was recovered from 257 of 479 (53.7%) retail garden products in this study. Prevalence was highest in turf (67.1%, 53/79), followed by soil mixes (57.1%, 148/259) and soil conditioners (39.7%, 56/141). Of the 113 distinct ribotypes identified, the most common were 014/020 and QX 686. Toxigenic ribotypes associated with both human infection and production animals in Australia, including 014/020, 056 and 087, were recovered.

Conclusion: A high prevalence of C. difficile was found in garden products sold to consumers, including toxigenic strains associated with C. difficile infection in humans and animals. More discriminatory characterisation of isolates is required to determine if retail garden products contribute to the burden of community-associated C. difficile infection in Australia.

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来源期刊
Anaerobe
Anaerobe 生物-微生物学
CiteScore
5.20
自引率
8.70%
发文量
137
审稿时长
76 days
期刊介绍: Anaerobe is essential reading for those who wish to remain at the forefront of discoveries relating to life processes of strictly anaerobes. The journal is multi-disciplinary, and provides a unique forum for those investigating anaerobic organisms that cause infections in humans and animals, as well as anaerobes that play roles in microbiomes or environmental processes. Anaerobe publishes reviews, mini reviews, original research articles, notes and case reports. Relevant topics fall into the broad categories of anaerobes in human and animal diseases, anaerobes in the microbiome, anaerobes in the environment, diagnosis of anaerobes in clinical microbiology laboratories, molecular biology, genetics, pathogenesis, toxins and antibiotic susceptibility of anaerobic bacteria.
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