Anaerobe最新文献_第10页

Cultivation of anaerobic bacteria: Foundations and principles 厌氧细菌的培养：基础和原理

IF 2.5 3区生物学

Anaerobe Pub Date : 2025-06-01 Epub Date: 2025-03-24 DOI: 10.1016/j.anaerobe.2025.102951

Paul A. Lawson, Ralph S. Tanner

引用次数: 0

Prevalence of toxigenic Clostridium botulinum in food products sold in Indian retail markets 在印度零售市场销售的食品中产毒梭菌肉毒杆菌的流行。

IF 2.5 3区生物学

Anaerobe Pub Date : 2025-06-01 Epub Date: 2025-03-19 DOI: 10.1016/j.anaerobe.2025.102954

Athira Vidyadharan, Arun Jyothi Puthiya Veettil, Athira Pulickal Santhosh, Kuttanapilly Velayudhan Lalitha, Toms Cheriyath Joseph

{"title":"Prevalence of toxigenic Clostridium botulinum in food products sold in Indian retail markets","authors":"Athira Vidyadharan, Arun Jyothi Puthiya Veettil, Athira Pulickal Santhosh, Kuttanapilly Velayudhan Lalitha, Toms Cheriyath Joseph","doi":"10.1016/j.anaerobe.2025.102954","DOIUrl":"10.1016/j.anaerobe.2025.102954","url":null,"abstract":"<div><h3>Introduction</h3><div><em>Clostridium botulinum</em> and botulinum neurotoxins have been recognized as an important food safety hazard. The objective of this study was to screen the prevalence of <em>C. botulinum</em> and botulinum toxin in various food products sold in the retail markets of India.</div></div><div><h3>Material and methods</h3><div>A total of 236 food products collected from Indian retail markets were screened for preformed botulinum toxin using a mouse bioassay. This was followed by enrichment and isolation and further testing of isolates for botulinum toxin production using an additional mouse bioassay. A toxin neutralization test with type specific antitoxins, 16S rRNA gene sequencing and <em>BoNT</em> (botulinum neurotoxin) gene amplification confirmed the presence of <em>C. botulinum</em> and its toxin production potential.</div></div><div><h3>Results and discussion</h3><div>Preformed botulinum toxin was not detected in any of the products. The overall prevalence of <em>C. botulinum</em> in the products was 11 %, but pure cultures of <em>C. botulinum</em> could be isolated only from 10 samples. The isolates were identified as <em>C. botulinum</em> type A, type B and type E based on toxin typing with type specific antitoxins and amplification of the botulinum neurotoxin (<em>BoNT)</em> gene. One of the isolates was designated as <em>C. botulinum</em> subtype A<sub>1</sub>B. This is the first report of isolation of <em>C. botulinum</em> type E and subtype A<sub>1</sub>B in seafood from India. The presence of <em>C. botulinum</em> in the products tested can be a significant public health hazard since the organism can grow when exposed to favourable conditions and can produce neurotoxin in the food.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"93 ","pages":"Article 102954"},"PeriodicalIF":2.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Obituary of Mike Cox (1945–2024) 迈克·考克斯讣告（1945-2024）

IF 2.5 3区生物学

Anaerobe Pub Date : 2025-06-01 Epub Date: 2025-03-22 DOI: 10.1016/j.anaerobe.2025.102956

Elisabeth Nagy, Ellen Jo Baron

引用次数: 0

A rare isolation of Parvimonas micra from cerebellar abscess in a patient with complex cyanotic heart disease 一个罕见的分离微小微小单胞菌小脑脓肿患者的复杂紫绀性心脏病。

IF 2.5 3区生物学

Anaerobe Pub Date : 2025-06-01 Epub Date: 2025-02-01 DOI: 10.1016/j.anaerobe.2025.102943

Praveen Ravichandran , Ramit Kundu , Rakhi Biswas , Sathiaprabhu Anbazhagan

引用次数: 0

CDBN-YGXZ shows highly antibacterial activity against Clostridioides difficile as a potential candidate drug CDBN-YGXZ对艰难梭菌具有较高的抑菌活性，是一种潜在的候选药物

IF 2.5 3区生物学

Anaerobe Pub Date : 2025-06-01 Epub Date: 2025-04-25 DOI: 10.1016/j.anaerobe.2025.102964

Xiaoxia Zhang , Min Quan , Xin Lu , Linwan Zhang , Bing Guang , Zhiyong Zong , Xiaohui Wang

引用次数: 0

An older man with Bifidobacterium breve bacteremia, a rare condition in adults: A case report 一个老年男子与短双歧杆菌菌血症，一个罕见的条件在成人：一个病例报告。

IF 2.5 3区生物学

Anaerobe Pub Date : 2025-06-01 Epub Date: 2025-04-26 DOI: 10.1016/j.anaerobe.2025.102968

Miku Muramatsu , Dai Akine , Teppei Sasahara , Takayuki Suzuki , Tomohiro Tamura , Takayuki Kaburagi , Shinya Watanabe

引用次数: 0

A metabolite dehydrogenase pathway represses sporulation of Clostridioides difficile 一种代谢物脱氢酶途径抑制艰难梭菌的产孢。

IF 2.5 3区生物学

Anaerobe Pub Date : 2025-06-01 Epub Date: 2025-05-09 DOI: 10.1016/j.anaerobe.2025.102971

Daniela Wetzel , Arshad Rizvi , Adrianne N. Edwards , Shonna M. McBride

{"title":"A metabolite dehydrogenase pathway represses sporulation of Clostridioides difficile","authors":"Daniela Wetzel , Arshad Rizvi , Adrianne N. Edwards , Shonna M. McBride","doi":"10.1016/j.anaerobe.2025.102971","DOIUrl":"10.1016/j.anaerobe.2025.102971","url":null,"abstract":"<div><h3>Objectives</h3><div><em>Clostridioides difficile</em> is a major gastrointestinal pathogen that is transmitted as a dormant spore. As an intestinal pathogen, <em>C. difficile</em> must contend with variable environmental conditions, including fluctuations in pH and nutrient availability. Nutrition and pH both influence growth and spore formation, but how pH and nutrition jointly influence sporulation are not known. In this study, we investigated the dual impact of pH and pH-dependent metabolism on <em>C. difficile</em> sporulation.</div></div><div><h3>Methods</h3><div>We examined the impacts of pH and the metabolite acetoin on <em>C. difficile</em> growth, gene expression, and sporulation.</div></div><div><h3>Results</h3><div>We found that expression of the predicted acetoin dehydrogenase operon, <em>CD0035-CD0039</em>, was pH-dependent and repressed by acetoin and pyruvate. Regulation of the <em>C. difficile CD0035-CD0039</em> locus is distinct from characterized orthologous systems and appears to involve a co-transcribed DeoR-family regulator, rather than a sigma<sup>54</sup>-dependent activator. In addition, an <em>CD0036</em> null mutant produced significantly more spores and initiated sporulation earlier than the parent strain. However, unlike other Firmicutes, growth and culture density of <em>C. difficile</em> was not increased by acetoin availability or disruption of the dehydrogenase pathway.</div></div><div><h3>Conclusions</h3><div>Together, these results indicate that acetoin, pH, and the <em>CD0036-CD0039</em> dehydrogenase pathway play important roles in nutritional repression of sporulation in <em>C. difficile</em>. However, the data do not support the involvement of the CD0036-CD0039 pathway in acetoin metabolism and acetoin is not a significant stationary phase energy source for <em>C. difficile</em>.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"93 ","pages":"Article 102971"},"PeriodicalIF":2.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143971075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular detection of toxigenic Clostridioides difficile in subgingival biofilm of severe periodontitis 重度牙周炎龈下生物膜中产毒艰难梭菌的分子检测。

IF 2.5 3区生物学

Anaerobe Pub Date : 2025-06-01 Epub Date: 2025-03-15 DOI: 10.1016/j.anaerobe.2025.102955

Isabela Leite de Oliveira Rosa, Eliane de Oliveira Ferreira, Ana Paula Vieira Colombo

{"title":"Molecular detection of toxigenic Clostridioides difficile in subgingival biofilm of severe periodontitis","authors":"Isabela Leite de Oliveira Rosa, Eliane de Oliveira Ferreira, Ana Paula Vieira Colombo","doi":"10.1016/j.anaerobe.2025.102955","DOIUrl":"10.1016/j.anaerobe.2025.102955","url":null,"abstract":"<div><h3>Objectives</h3><div>The oral cavity is the main gateway for the entry of <em>C. difficile</em> spores to the digestive tract. In conditions of poor oral hygiene and periodontal diseases, the dysbiotic oral microbiota may be a reservoir for several human pathogens. Here, we explored the prevalence of <em>C. difficile</em> in the oral microbiota of patients with severe periodontitis by the molecular detection of species specific genes.</div></div><div><h3>Methods</h3><div>Subgingival biofilm, saliva and/or feces from 659 patients with gingivitis, periodontitis and no periodontal diseases were screened for the <em>tpi</em> and toxin A/B genes specific for <em>C. difficile</em> by multiplex PCR. Differences among groups were sought by the Chi-square test.</div></div><div><h3>Results</h3><div>The overall frequency of <em>C. difficile tpi</em> gene was 29 %, with a high detection of <em>tcd</em>B gene (44.8 %). Patients with periodontitis showed a greater prevalence of this gene in the biofilm than individuals with gingivitis and periodontal health (p = 0.001), particularly at more severe stages of disease (p < 0.05). No toxin genes were detected in feces or biofilm from healthy patients, whereas >70 % of the biofilm from patients with periodontal diseases were positive for these genes (p < 0.001). Detection of <em>C. difficile tpi</em> gene in oral/fecal samples correlated with periodontal inflammation (p < 0.05). A modest intra-individual agreement between <em>tpi</em> gene detection in feces and saliva was found within periodontitis patients (Kappa = 0.314; p = 0.003).</div></div><div><h3>Conclusion</h3><div>The high frequency of the <em>C. difficile</em> specific genes <em>tpi</em> and <em>tcd</em>B in the dysbiotic subgingival biofilm of advanced periodontitis could support the presence of the bacterium in this niche.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"93 ","pages":"Article 102955"},"PeriodicalIF":2.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143647230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel competitive annealing-mediated isothermal amplification (CAMP)-based detection of epsilon toxin gene in Clostridium perfringens 基于竞争退火介导的等温扩增（CAMP）检测产气荚膜梭菌epsilon毒素基因的新方法。

IF 2.5 3区生物学

Anaerobe Pub Date : 2025-06-01 Epub Date: 2025-03-26 DOI: 10.1016/j.anaerobe.2025.102958

A. Arun Prince Milton , Torik Basar , K. Srinivas , Aleimo G. Momin , Sabia Khan , G. Bhuvana Priya , Samir Das , S. Ghatak , K. Puro

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Development of a polymicrobial host-bacterial interface biofilm model for bacterial vaginosis 细菌性阴道病多微生物宿主-细菌界面生物膜模型的建立。

IF 2.5 3区生物学

Anaerobe Pub Date : 2025-06-01 Epub Date: 2025-03-08 DOI: 10.1016/j.anaerobe.2025.102952

William Johnston, Ryan Kean

引用次数: 0