Anaerobe最新文献

{"title":"Description of eight human infections caused by Bacteroides pyogenes in a tertiary hospital of northern Spain","authors":"Domingo Fernández Vecilla ,&nbsp;Mikel Joseba Urrutikoetxea Gutiérrez ,&nbsp;Mary Paz Roche Matheus ,&nbsp;Itziar Angulo López ,&nbsp;Cristina Aspichueta Vivanco ,&nbsp;Felicitas Elena Calvo Muro ,&nbsp;José Luis Díaz de Tuesta del Arco","doi":"10.1016/j.anaerobe.2023.102759","DOIUrl":"10.1016/j.anaerobe.2023.102759","url":null,"abstract":"<div><p><span><em>Bacteroides</em><em> pyogenes</em></span><span> is a Gram-negative obligate anaerobe, saccharolytic, non-motile, non-pigment-producing and non-spore-forming rod. Reports of human infections caused by </span><em>B. pyogenes</em> are scarce, with approximately 30 cases documented in scientific literature. The aim of this study was to describe the clinical characteristics of 8 different patients and to study the <em>in vitro</em><span> antibiotic susceptibility of the strains, as well as to evaluate the </span><em>in vivo</em><span> activity of the prescribed treatments. We conducted a descriptive retrospective study by searching all </span><em>B. pyogenes</em><span><span><span> isolates from January 2010 to March 2023 at the Basurto University Hospital. This included all cases, both in monomicrobial or polymicrobial cultures. Among the eight patients, three experienced severe infections such as bacteremia and </span>osteomyelitis. All the strains were susceptible to amoxicillin/clavulanic acid, piperacillin/tazobactam, </span>imipenem<span><span>, meropenem, </span>clindamycin<span><span>, metronidazole and </span>moxifloxacin.</span></span></span></p></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9985599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spore-forming properties and enhanced oxygen tolerance of butyrate-producing Anaerostipes spp.","authors":"Ren Kadowaki ,&nbsp;Hiroki Tanno ,&nbsp;Shintaro Maeno ,&nbsp;Akihito Endo","doi":"10.1016/j.anaerobe.2023.102752","DOIUrl":"10.1016/j.anaerobe.2023.102752","url":null,"abstract":"<div><h3>Objectives</h3><p><span><span>Butyrate producing bacteria are promising candidates for next-generation </span>probiotics. However, they are extremely sensitive to oxygen, which is a significant obstacle to their inclusion in food matrices in a viable form. The present study characterized the spore-forming properties and stress tolerance of human gut butyrate-producing </span><em>Anaerostipes</em> spp.</p></div><div><h3>Methods</h3><p><span>Spore formation properties in six species of </span><em>Anaerostipes</em> spp. were studied by <em>in vitro</em> and <span><em>in silico</em></span> tests.</p></div><div><h3>Results</h3><p><span>Spores were observed from the cells of three species using microscopic analyses, while the remaining three did not form spores under the tested conditions. Spore-forming properties were confirmed by an ethanol treatment. The spores of </span><em>Anaerostipes caccae</em> were tolerant to oxygen and survived for 15 weeks under atmospheric conditions. Spores tolerated heat stress at 70 °C, but not at 80 °C. An <em>in silico</em><span> analysis of the conservation of potential sporulation signature genes revealed that the majority of human gut butyrate-producing bacteria were classified as potential spore formers. Comparative genomics revealed that three spore-forming </span><em>Anaerostipes</em> spp. specifically possessed the spore formation-related genes of <em>bkdR, sodA,</em> and <em>splB</em>, which may be key genes for different sporulation properties in <em>Anaerostipes</em> spp.</p></div><div><h3>Conclusions</h3><p>The present study demonstrated the enhanced stress tolerance of butyrate producing <em>Anaerostipes</em> spp. for future probiotic application. Presence of specific gene(s) are possibly keys for sporulation in <em>Anaerostipes</em> spp.</p></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9994515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Type IV pili facilitated natural competence in Fusobacterium nucleatum","authors":"Blake E. Sanders,&nbsp;Ariana Umaña,&nbsp;Tam T.D. Nguyen,&nbsp;Kevin J. Williams,&nbsp;Christopher C. Yoo,&nbsp;Michael A. Casasanta,&nbsp;Bryce Wozniak,&nbsp;Daniel J. Slade","doi":"10.1016/j.anaerobe.2023.102760","DOIUrl":"10.1016/j.anaerobe.2023.102760","url":null,"abstract":"<div><h3>Objectives</h3><p>Many bacterial species naturally take up DNA from their surroundings and recombine it into their chromosome through homologous gene transfer (HGT) to aid in survival and gain advantageous functions. Herein we present the first characterization of Type IV pili facilitated natural competence in <em>Fusobacterium nucleatum</em>, which is a Gram-negative, anaerobic bacterium that participates in a range of infections and diseases including periodontitis, preterm birth, and cancer<em>.</em></p></div><div><h3>Methods</h3><p>Here we used bioinformatics on multiple <em>Fusobacterium</em> species, as well as molecular genetics to characterize natural competence in strain <em>F. nucleatum</em> subsp. <em>nucleatum ATCC 23726.</em></p></div><div><h3>Results</h3><p>We bioinformatically identified components of the Type IV conjugal pilus machinery and show this is a conserved system within the <em>Fusobacterium</em> genus. We next validate Type IV pili in natural competence in <em>F. nucleatum</em> ATCC 23726 and show that gene deletions in key components of pilus deployment (<em>pilQ)</em> and cytoplasmic DNA import (<em>comEC)</em> abolish DNA uptake and chromosomal incorporation. We next show that natural competence may require native <em>F. nucleatum</em> DNA methylation to bypass restriction modification systems and allow subsequent genomic homologous recombination.</p></div><div><h3>Conclusions</h3><p>In summary, this proof of principle study provides the first characterization of natural competence in <em>Fusobacterium nucleatum</em> and highlights the potential to exploit this DNA import mechanism as a genetic tool to characterize virulence mechanisms of an opportunistic oral pathogen.</p></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10306940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The challenges of diagnosis and treatment of rare Prevotella-induced breast abscesses: A retrospective cohort study","authors":"I.M. Brouwer de Koning ,&nbsp;S.W.T. Hoogmoet ,&nbsp;N.H.M. Renders ,&nbsp;Y.C.G.J. Paquay ,&nbsp;M. Bessems ,&nbsp;W.A. Draaisma ,&nbsp;K. Bosscha","doi":"10.1016/j.anaerobe.2023.102763","DOIUrl":"10.1016/j.anaerobe.2023.102763","url":null,"abstract":"<div><h3>Objective</h3><p>Although the genus <span><em>Prevotella</em></span><span><span> is part of the general human microbiota, species of this anaerobic gram-negative bacterium have been described as causes of persisting nonpuerperal breast abscesses<span>. Collecting punctate samples and testing these samples for anaerobic bacteria is not part of the common diagnostic workflow in atypical breast abscesses. The causative anaerobic micro-organism can remain unclear and patients can be treated with multiple inadequate antibiotics and/or extensive surgical procedures. The aim of this </span></span>cohort study of </span><em>Prevotella</em><span> induced breast abscesses is to gain more insights into the diagnostic procedures and treatment.</span></p></div><div><h3>Methods</h3><p>Medical charts of patients with a <em>Prevotella</em><span> induced breast abscess between 2015 and 2021, were retrospectively reviewed on patient characteristics, diagnostic procedures, treatment and outcome.</span></p></div><div><h3>Results</h3><p>Twenty-one patients were included. Six subspecies of <em>Prevotella</em><span> were determined by culturing. High susceptibility was observed for amoxicillin/clavulanic acid (100%, n = 12). Nine patients (43%) were treated with antibiotics, eight patients (38%) with antibiotics and incision and drainage<span>, and four patients (19%) with only incision and drainage. Recurrence was observed in nine patients (43%), of whom five patients were treated with antibiotics and three patients had surgery. The mean duration of antibiotic administration in patients with recurrence was significantly shorter compared to those without recurrence (5.6 days vs. 19.5 days, p = 0.039).</span></span></p></div><div><h3>Conclusion</h3><p>Specific anaerobic culturing should be common practice in atypical breast abscesses to confirm <em>Prevotella</em> species. The high recurrence rate emphasizes the need of further research for optimal treatment. Prolonged duration of antibiotics could be considered and amoxicillin/clavulanic acid seems to be the first choice.</p></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10040071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strain specificity in fusobacterial co-aggregation with colorectal cancer-relevant species","authors":"Avery V. Robinson,&nbsp;Emma Allen-Vercoe","doi":"10.1016/j.anaerobe.2023.102758","DOIUrl":"10.1016/j.anaerobe.2023.102758","url":null,"abstract":"<div><h3>Objectives</h3><p>The purpose of the present study was to characterize co-aggregation interactions between isolates of <span><em>Fusobacterium nucleatum</em></span> subsp. <em>animalis</em> and other colorectal cancer (CRC)-relevant species.</p></div><div><h3>Methods</h3><p><span>Co-aggregation interactions were assessed by comparing optical density values following 2-h stationary strain co-incubations to strain optical density values when incubated alone. Co-aggregation was characterized between strains from a previously isolated, CRC biopsy-derived community and </span><em>F. nucleatum</em> subsp. <em>animalis</em>, a species linked to CRC and known to be highly aggregative. Interactions were also investigated between the fusobacterial isolates and strains sourced from alternate human gastrointestinal samples whose closest species match aligned with species in the CRC biopsy-derived community.</p></div><div><h3>Results</h3><p>Co-aggregation interactions were observed to be strain-specific, varying between both <em>F. nucleatum</em> subsp. <em>animalis</em> strains and different strains of the same co-aggregation partner species. <em>F. nucleatum</em> subsp. <em>animalis</em> strains were observed to co-aggregate strongly with several taxa linked to CRC: <span><em>Campylobacter concisus</em></span>, <span><em>Gemella</em></span> spp., <em>Hungatella hathewayi</em>, and <span><em>Parvimonas micra</em></span>.</p></div><div><h3>Conclusions</h3><p>Co-aggregation interactions suggest the ability to encourage the formation of biofilms, and colonic biofilms, in turn, have been linked to promotion and/or progression of CRC. Co-aggregation between <em>F. nucleatum</em> subsp. <em>animalis</em> and CRC-linked species such as <em>C. concisus</em>, <em>Gemella</em> spp., <em>H. hathewayi</em>, and <em>P. micra</em><span> may contribute to both biofilm formation along CRC lesions and to disease progression.</span></p></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10210446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biology activity and characterization of the functional L-HN fragment derivative of botulinum neurotoxin serotype E","authors":"Xiao Tan ,&nbsp;Cong-Cong Zhang ,&nbsp;Jian-Sheng Lu ,&nbsp;Zhi-Ying Li ,&nbsp;Bo-Lin Li ,&nbsp;Xu-Yang Liu ,&nbsp;Yun-Zhou Yu ,&nbsp;Qing Xu","doi":"10.1016/j.anaerobe.2023.102764","DOIUrl":"10.1016/j.anaerobe.2023.102764","url":null,"abstract":"<div><h3>Objectives</h3><p><span><span>The mature botulinum neurotoxin (BoNT) is a long peptide chain consisting of a light chain (L) and a heavy chain (H) linked by a </span>disulfide bond, where the heavy chain is divided into a translocation domain and an acceptor binding domain (Hc). In this study, we further explored the biology activity and characteristics of recombinant L-HN fragment (EL-HN) composed of the L and HN domains of BoNT/E </span><em>in vivo</em> and <em>in vitro</em>.</p></div><div><h3>Methods</h3><p><span>Neurotoxicity of L-HN fragments from botulinum neurotoxins was assessed in mice. Cleavage of dichain EL-HN </span><em>in vitro</em><span> and in neuro-2a cells was assessed and compared with that of single chain EL-HN. Interaction of HN domain and the receptor synaptic vesicle<span> glycoprotein 2C (SV2C) was explored </span></span><em>in vitro</em> and in neuro-2a cells only expressing SV2C.</p></div><div><h3>Results</h3><p><span>We found that the 50% mouse lethal dose of the nicked dichain EL-HN fragment (EL–HN–DC) was 0.5 μg and its neurotoxicity was the highest among the L-HN's of the four serotypes<span> of BoNT (A/B/E/F). The cleavage efficiency of EL–HN–DC toward synaptosome associated protein 25 (SNAP25) </span></span><em>in vitro</em> was 3-fold higher than that of the single chain at the cellular level, and showed 200-fold higher animal toxicity. The EL–HN–DC fragment might enter neuro-2a cells via binding to SV2C to efficiently cleave SNAP25.</p></div><div><h3>Conclusions</h3><p>The EL-HN fragment showed good biological activities <em>in vivo</em> and <em>in vitro</em>, and could be used as a drug screening model and to further explore the molecular mechanism of its transmembrane transport.</p></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10220344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Membrane vesicles of Lactobacillus gasseri ATCC 19992 disrupt biofilms of vaginal pathogens","authors":"Sameen Khan ,&nbsp;Parul Shishpal ,&nbsp;Vikrant M. Bhor","doi":"10.1016/j.anaerobe.2023.102761","DOIUrl":"10.1016/j.anaerobe.2023.102761","url":null,"abstract":"<div><p><span>Membrane vesicles (MVs) are bioactive, nano-sized entities produced by all organisms. MVs of </span><em>L. gasseri</em><span> ATCC 19992 were isolated and their effect on the biofilms of vaginal pathogens, </span><em>G. vaginalis</em> and <span><em>S. aureus</em></span> was studied. The <em>L. gasseri</em> MVs resulted in significant disruption of biofilms of the vaginal pathogens.</p></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10234073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative evaluation of MALDI-TOF MS and 16S rRNA gene sequencing for the identification of clinically relevant anaerobic bacteria: critical evaluation of discrepant results","authors":"Fernando Cobo,&nbsp;Virginia Pérez-Carrasco,&nbsp;Lina Martín-Hita,&nbsp;José Antonio García-Salcedo,&nbsp;José María Navarro-Marí","doi":"10.1016/j.anaerobe.2023.102754","DOIUrl":"10.1016/j.anaerobe.2023.102754","url":null,"abstract":"<div><h3>Objectives</h3><p>The main study objective was to evaluate the correlation between matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and 16S rRNA gene sequencing results for the identification of anaerobes.</p></div><div><h3>Methods</h3><p>A retrospective study was conducted of all anaerobic bacteria isolated from clinically significant specimens. MALDI-TOF (Bruker Byotyper) and 16S rRNA gene sequencing were performed in all strains. Identifications were considered correct when the concordance with gene sequencing was ≥99%.</p></div><div><h3>Results</h3><p>The study included 364 isolates of anaerobic bacteria: 201 (55.2%) Gram-negative and 163 (44.8%) Gram-positive, mostly belonging to the genus <em>Bacteroides</em>. Isolates were largely obtained from blood cultures (128/35.4%) and intra-abdominal samples (116/32.1%). Overall, 87.3% of isolates were identified at species level using the version 9 database (89.5% of Gram-negative and 84.6% of Gram-positive anaerobic bacteria). All isolates belonging to the species <em>B. fragilis sensu stricto</em> were correctly identified by MALDI-TOF MS, but five cases of <em>Phocaeicola (Bacteroides) dorei</em> were misidentified as <em>Phocaeicola</em> (<em>Bacteroides) vulgatus;</em> all <em>Prevotella</em> isolates were correctly identified at the genus level, and most were correctly identified at the species level. Among Gram-positive anaerobes, 12 <em>Anaerococcus</em> species were not identified by MALDI-TOF MS, while six cases identified as <em>Peptoniphilus indolicus</em> were found to belong to other genera/species.</p></div><div><h3>Conclusions</h3><p>MALDI-TOF is a reliable technique for identifying most anaerobic bacteria, although the database needs frequent updating to identify rare, infrequent, and newly discovered species.</p></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9658921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prosthetic joint infection caused by an atypical gram-negative bacilli: Odoribacter splanchnicus","authors":"Yuri Lara-Taranchenko ,&nbsp;Pablo S. Corona ,&nbsp;Dolors Rodríguez-Pardo ,&nbsp;Paula Salmerón-Menéndez ,&nbsp;Marina Vicente Ciurans ,&nbsp;María Cristina García-Martínez ,&nbsp;Lluís Carrera Calderer","doi":"10.1016/j.anaerobe.2023.102740","DOIUrl":"10.1016/j.anaerobe.2023.102740","url":null,"abstract":"<div><p><span><span><span>Prosthetic joint infection (PJI) is a devastating complication after total hip arthroplasty. Its management consists of both: a radical </span>debridement and implant retention or exchange (depending on the timing of symptoms) and directed antibiotic therapy. Thus, the isolation of atypical microorganisms implies a challenge, where </span>anaerobes are responsible for only 4% of cases. However, </span><em>Odoribacter splanchnicus</em> has not been reported as a cause of PJI yet.</p><p>We present an 82 year-old woman who was diagnosed with hip PJI. A radical debridement, prosthetic withdrawal, and spacer introduction was performed. Despite the directed antibiotic therapy against <em>E. coli</em> which was first isolated, the patient persisted clinically febrile. An anaerobic Gram-negative rod was isolated and finally, <em>Odoribacter splanchnicus</em><span><span> was identified and confirmed by 16S rRNA gene sequencing. Then, antibiotic bitherapy with </span>ciprofloxacin<span> and metronidazole was started until 6 weeks after surgery. The patient had no signs of infection recurrence after then.</span></span></p><p>This case report also shows the importance of genomic identification of rare microorganisms causing PJI, and also allows setting a directed antibiotic therapy which is crucial for infection eradication.</p></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9664570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of microbiological profile of enterotoxigenic Bacteroides fragilis (ETBF) isolates from subjects with colorectal cancer (CRC) or intestinal pre-cancerous lesions versus healthy individuals and evaluation of environmental factors involved in intestinal dysbiosis","authors":"Patrizia Spigaglia ,&nbsp;Fabrizio Barbanti ,&nbsp;Elena Angela Pia Germinario ,&nbsp;Enrico Maria Criscuolo ,&nbsp;Giovanni Bruno ,&nbsp;Lupe Sanchez-Mete ,&nbsp;Barbara Porowska ,&nbsp;Vittoria Stigliano ,&nbsp;Fabio Accarpio ,&nbsp;Andrea Oddi ,&nbsp;Ilaria Zingale ,&nbsp;Silvia Rossi ,&nbsp;Roberta De Angelis ,&nbsp;Alessia Fabbri","doi":"10.1016/j.anaerobe.2023.102757","DOIUrl":"10.1016/j.anaerobe.2023.102757","url":null,"abstract":"<div><h3>Objective</h3><p>The aim of this study was to analyze enterotoxigenic <span><em>Bacteroides fragilis</em></span><span> (ETBF) isolates from colorectal biopsies of subjects with a histological analysis positive for colorectal cancer (CRC), pre-cancerous lesions (pre-CRC) or with a healthy intestinal tissue and to evaluate the environmental factors<span> that may not only concur to CRC development but may also affect gut microbiota composition.</span></span></p></div><div><h3>Methods</h3><p>ETBF isolates were typed using the ERIC-PCR method, while PCR assays were performed to investigate the <em>bft</em> alleles, the <em>B. fragilis</em><span> pathogenicity island (BFPAI) region and the </span><em>cepA, cfiA</em> and <em>cfxA</em><span> genes. Susceptibility to antibiotics was tested using the agar dilution<span> method. Environmental factors that could play a role in promoting intestinal dysbiosis were evaluated throughout a questionnaire administered to the subjects enrolled.</span></span></p></div><div><h3>Results</h3><p>Six different ERIC-PCR types were identified. The type denominated C in this study was the most prevalent, in particular among the biopsies of subjects with pre-CRC, while an isolate belonging to a different type, denominated F, was detected in a biopsy from a subject with CRC. All the ETBF isolates from pre-CRC or CRC subjects had a <em>B. fragilis</em> pathogenicity island (BFPAI) region pattern I, while those from healthy individuals showed also different patterns. Furthermore, 71% of isolates from subjects with pre-CRC or CRC were resistant to two or more classes of antibiotics vs 43% of isolates from healthy individuals. The <em>B. fragilis</em><span> toxin BFT1 was the most frequently detected in this study, confirming the constant circulation of this isoform strains in Italy. Interestingly, BFT1 was found in 86% of the ETBF isolates from patients with CRC or pre-CRC, while the BFT2 was prevalent among the ETBF isolates from healthy subjects. No substantial differences based on sex, age, tobacco and alcohol consumption were observed between healthy and non-healthy individuals included in this study, while most of the subjects with CRC or pre-CRC lesions were subjected to pharmacological therapy (71%) and showed a body mass index (BMI) that falls within the overweight range (86%).</span></p></div><div><h3>Conclusions</h3><p>Our data suggest that some types of ETBF seem to better adapt and colonize the human gut and that the selective pressure exerted by factors related to lifestyle, such as pharmacological therapy and weight, could facilitate their persistence in the gut and their possible involvement in CRC development.</p></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9719756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}