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Ferviditalea candida gen. nov., sp. nov., a novel member of the family Paenibacillaceae isolated from a geothermal area Ferviditalea candida gen.
IF 2.5 3区 生物学
Anaerobe Pub Date : 2024-05-24 DOI: 10.1016/j.anaerobe.2024.102866
Ying Chen , Ai-Ping Lv , Meng-Meng Li , Yu-Ting OuYang , Zheng-Han Lian , Le-Bin Chen , Ze-Tao Liu , Lan Liu , Jian-Yu Jiao , Wen-Jun Li
{"title":"Ferviditalea candida gen. nov., sp. nov., a novel member of the family Paenibacillaceae isolated from a geothermal area","authors":"Ying Chen ,&nbsp;Ai-Ping Lv ,&nbsp;Meng-Meng Li ,&nbsp;Yu-Ting OuYang ,&nbsp;Zheng-Han Lian ,&nbsp;Le-Bin Chen ,&nbsp;Ze-Tao Liu ,&nbsp;Lan Liu ,&nbsp;Jian-Yu Jiao ,&nbsp;Wen-Jun Li","doi":"10.1016/j.anaerobe.2024.102866","DOIUrl":"10.1016/j.anaerobe.2024.102866","url":null,"abstract":"<div><h3>Objective</h3><p>The family <em>Paenibacillaceae</em> is linked to the order <em>Caryophanales</em>. <em>Paenibacillaceae</em> members residing in compost or soil play crucial roles in nutrient recycling and breaking down complex organic materials. However, our understanding of <em>Paenibacillaceae</em> remains limited.</p></div><div><h3>Methods</h3><p>Strain SYSU GA230002<sup>T</sup> was conclusively identified using a polyphasic taxonomic approach frequently utilized in bacterial systematics. Standard microbiological techniques were employed to characterize the morphology and biochemistry of strain SYSU GA230002<sup>T</sup>.</p></div><div><h3>Results</h3><p>An anaerobic and gram--negative bacterium, designated SYSU GA230002<sup>T</sup>, was isolated from geothermally heated soil of Tengchong, Yunnan Province, south-west China. Phylogenetic analyses based on 16S rRNA gene sequences and genomes showed that strain SYSU GA230002<sup>T</sup> belongs to the family <em>Paenibacillaceae</em>. 16S rRNA gene sequence similarity (&lt;94.0 %), ANI (&lt;71.95 %) and AAI values (&lt;58.67 %) between strain SYSU GA230002<sup>T</sup> with other members of the family were lower than the threshold values recommended for distinguishing novel species. Growth was observed at 30-45 °C (optimum, 37 °C), pH 7.0–8.0 (optimum, pH 7.5) and in 0–3.0 % (w/v) NaCl concentrations (optimum, 0 %). The major fatty acids detected were anteiso-C<sub>15:0</sub>, iso-C<sub>16:0</sub> and iso-C<sub>17:0</sub>. The polar lipids included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, one unidentified phospholipid, one unidentified aminolipid and two unidentified glycolipids. The respiratory quinone was MK-7. The DNA G + C content of strain SYSU GA230002<sup>T</sup> was 49.87 %.</p></div><div><h3>Conclusion</h3><p>Based on the results of morphological, physiological properties, and chemotaxonomic characteristics, this strain is proposed to represent a new species of a new genus <em>Ferviditalea candida</em> gen. nov., sp. nov. The type strain of the type species is SYSU GA230002<sup>T</sup> (=KCTC 25726<sup>T</sup> = GDMCC 1.4160<sup>T</sup>).</p></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"88 ","pages":"Article 102866"},"PeriodicalIF":2.5,"publicationDate":"2024-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141134586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Clostridioides difficile infection in patients with and without COVID-19 during the pandemic: A retrospective cohort study from a tertiary referral hospital 大流行期间感染和未感染 COVID-19 的艰难梭菌患者:一家三级转诊医院的回顾性队列研究。
IF 2.3 3区 生物学
Anaerobe Pub Date : 2024-05-14 DOI: 10.1016/j.anaerobe.2024.102864
Alexandra Tsankof , Adonis A. Protopapas , Paraskevi Mantzana , Efthymia Protonotariou , Lemonia Skoura , Andreas N. Protopapas , Christos Savopoulos , Konstantinos Mimidis
{"title":"Clostridioides difficile infection in patients with and without COVID-19 during the pandemic: A retrospective cohort study from a tertiary referral hospital","authors":"Alexandra Tsankof ,&nbsp;Adonis A. Protopapas ,&nbsp;Paraskevi Mantzana ,&nbsp;Efthymia Protonotariou ,&nbsp;Lemonia Skoura ,&nbsp;Andreas N. Protopapas ,&nbsp;Christos Savopoulos ,&nbsp;Konstantinos Mimidis","doi":"10.1016/j.anaerobe.2024.102864","DOIUrl":"10.1016/j.anaerobe.2024.102864","url":null,"abstract":"<div><h3>Objectives</h3><p>This study aims to detect the prevalence and specific characteristics of <em>Clostridioides difficile</em> infection (CDI) during the COVID-19 pandemic.</p></div><div><h3>Methods</h3><p>In this retrospective observational study, conducted in a tertiary hospital in Greece between May 2021 and October 2022, patients with CDI from COVID-19 and Internal Medicine wards were enrolled and compared based on epidemiological and disease-associated data.</p></div><div><h3>Results</h3><p>In total, 4322 patients were admitted, and 435 samples for CDI were analyzed, with 104/435 (23.9 %) sample positivity and 2.4 % prevalence. We observed an increased prevalence of CDI compared to the beginning of the COVID-19 pandemic (prevalence = 1.7 %, p = 0.003). 35.6 % of the CDI patients were hospitalized in the COVID-19 ward and 64.4 % in the Internal Medicine ward. COVID-19 patients were younger (p = 0.02) with a lower Charlson Comorbidity Index (CCI) compared to the Internal Medicine ward patients (p &lt; 0.001). With regards to the origin of CDI cases, in the Internal Medicine ward, 68.7 % presented with Hospital-Onset CDI, 17.9 % with Community Onset-Healthcare Associated CDI and 13.4 % with Community Associated CDI, while in the COVID-19 ward, the respective percentages were 86.5 %, 5.4 % and 8.1 %. Finally, there was an increased CDI-related CFR (Case Fatality Ratio) in the Internal Medicine ward compared to the COVID-19 ward (28.4 % vs. 5.4 %, p = 0.001).</p></div><div><h3>Conclusions</h3><p>Increased CDI prevalence and testing were observed compared to the beginning of the COVID-19 pandemic. Lower CDI-related CFR was observed in patients with COVID-19, which may be credited to the patients’ significantly lower median age and CCI, as well as to the majority of deaths being due to respiratory failure.</p></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"88 ","pages":"Article 102864"},"PeriodicalIF":2.3,"publicationDate":"2024-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140955911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fecal shedding of Clostridioides difficile in calves in Sao Paulo state, Brazil 巴西圣保罗州犊牛粪便中艰难梭菌的散播。
IF 2.5 3区 生物学
Anaerobe Pub Date : 2024-05-09 DOI: 10.1016/j.anaerobe.2024.102861
Fabrício Moreira Cerri , Roberta Martins Basso , Wanderson Adriano Biscola Pereira , Júlia Meireles da Silva Silveira , Eliane de Oliveira Ferreira , Amanda Haisi , João Pessoa Araújo Júnior , Luis G. Arroyo , Yasmin Gonçalves de Castro , Rodrigo Otávio Silveira Silva , José Paes de Oliveira-Filho , Alexandre Secorun Borges
{"title":"Fecal shedding of Clostridioides difficile in calves in Sao Paulo state, Brazil","authors":"Fabrício Moreira Cerri ,&nbsp;Roberta Martins Basso ,&nbsp;Wanderson Adriano Biscola Pereira ,&nbsp;Júlia Meireles da Silva Silveira ,&nbsp;Eliane de Oliveira Ferreira ,&nbsp;Amanda Haisi ,&nbsp;João Pessoa Araújo Júnior ,&nbsp;Luis G. Arroyo ,&nbsp;Yasmin Gonçalves de Castro ,&nbsp;Rodrigo Otávio Silveira Silva ,&nbsp;José Paes de Oliveira-Filho ,&nbsp;Alexandre Secorun Borges","doi":"10.1016/j.anaerobe.2024.102861","DOIUrl":"10.1016/j.anaerobe.2024.102861","url":null,"abstract":"<div><h3>Objective</h3><p>This study aimed to evaluate the fecal shedding of <em>C. difficile</em> in calves on farms in Sao Paulo State, Brazil.</p></div><div><h3>Materials and methods</h3><p>Fecal samples (n = 300) were collected from diarrheic (n = 78) and nondiarrheic (n = 222) calves less than 60 days of age from 20 farms. Fecal samples were inoculated into enrichment broth supplemented with taurocholate and cultured under anaerobic conditions. Colonies suspected to be <em>C. difficile</em> were harvested for DNA extraction and then multiplex PCR for the detection of genes encoding toxins A and B and binary toxins. All toxigenic isolates were ribotyped and tested for antimicrobial susceptibility, and five selected strains were subjected to whole-genome sequencing to determine their sequence type.</p></div><div><h3>Results and discussion</h3><p><em>C. difficile</em> was isolated from 29.3 % (88/300) of the samples. All toxigenic isolates (17/88, 19.3 %) were classified as ribotypes RT046 (13/17–79.47 %, A<sup>+</sup>B<sup>+</sup> CDT<sup>−</sup>) and RT126 (4/17 = 20.53 %, A<sup>+</sup>B<sup>+</sup> CDT<sup>+</sup>). The sequenced strains from RT046 were classified as ST35 (Clade 1), while those from RT126 were classified as ST11 (Clade 5). No associations between the epidemiological factors in any of the groups and <em>C. difficile</em> isolation were observed. Most of the toxigenic isolates (16/17 = 94.41 %) were classified as multidrug-resistant. Calves can be an important source of toxigenic <em>C. difficile</em> strains, including multidrug-resistant isolates from ribotypes commonly observed in humans.</p></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"88 ","pages":"Article 102861"},"PeriodicalIF":2.5,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140904022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bacteroides fragilis spondylitis after suspected oral ulcer: a clinical case and comprehensive literature review 疑似口腔溃疡后的脆弱脊柱炎杆菌:临床病例与文献综述。
IF 2.5 3区 生物学
Anaerobe Pub Date : 2024-05-07 DOI: 10.1016/j.anaerobe.2024.102863
{"title":"Bacteroides fragilis spondylitis after suspected oral ulcer: a clinical case and comprehensive literature review","authors":"","doi":"10.1016/j.anaerobe.2024.102863","DOIUrl":"10.1016/j.anaerobe.2024.102863","url":null,"abstract":"<div><p>This paper reports a case of <em>Bacteroides fragilis</em> induced spondylitis. Diagnosis was confirmed through blood culture and metagenomic sequencing of pus for pathogen detection. Due to persistent lumbar pain, surgical intervention became imperative, resulting in favorable postoperative outcomes. A detailed patient history revealed a severe episode of oral ulceration two weeks before symptom onset, although a direct link to the infection remained elusive. Leveraging insights from this case, we conducted a comprehensive literature review on <em>B. fragilis</em> spondylitis, elucidating clinical manifestations, diagnostic methodologies, and therapeutic strategies.</p></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"88 ","pages":"Article 102863"},"PeriodicalIF":2.5,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1075996424000465/pdfft?md5=0fd607161dd8fc57fa19d7d13d9575af&pid=1-s2.0-S1075996424000465-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140890840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bacteroides vicugnae sp. nov. isolated from the fecal material of an alpaca 从羊驼排泄物中分离出的 Bacteroides vicugnae sp.
IF 2.3 3区 生物学
Anaerobe Pub Date : 2024-05-07 DOI: 10.1016/j.anaerobe.2024.102862
Samuel Miller , Meredith Hendry , Jacobey King , Krithivasan Sankaranarayanan , Paul A. Lawson
{"title":"Bacteroides vicugnae sp. nov. isolated from the fecal material of an alpaca","authors":"Samuel Miller ,&nbsp;Meredith Hendry ,&nbsp;Jacobey King ,&nbsp;Krithivasan Sankaranarayanan ,&nbsp;Paul A. Lawson","doi":"10.1016/j.anaerobe.2024.102862","DOIUrl":"10.1016/j.anaerobe.2024.102862","url":null,"abstract":"<div><p>Two strictly anaerobic, Gram-stain-negative rod-shaped bacterial isolates, A2-P53<sup>T</sup> and A1-P5, were isolated from an enrichment of fecal material from two alpacas (<em>Vicugna pacos)</em>. Based on a comparative 16S rRNA gene sequence analysis, the isolates were assigned to the genus <em>Bacteroides</em> with the highest sequence similarities to <em>Bacteroides koreensis</em> YS-aM39T (A2- P53T 97.7 % and A1-P5 97.9 %). Additionally, the average nucleotide identity and digital DNA-DNA hybridization values between these isolates and their closest relatives within <em>Bacteroides</em> were less than 92.1 % and 49.1 %, respectively. The average nucleotide identity between isolates A2-P53<sup>T</sup> and A1-P5 was 99.9 %. The predominant cellular fatty acid for isolates A2-P53<sup>T</sup> and A1-P5 was C<sub>15:0</sub> antesio. The G+C % content of the isolates was 41.7 %. Based on biochemical, phylogenetic, genotypic, and chemotaxonomic criteria, these isolates A2-P53<sup>T</sup> and A1-P5 represent two individual strains of a novel species within the genus <em>Bacteroides</em> for which the name <em>Bacteroides vicugnae</em> sp. nov. is proposed. The type strain of this species is strain A2-P53<sup>T</sup> (CCUG 77273<sup>T</sup> = CCM 9377<sup>T</sup> = NRRL B-65693<sup>T</sup>).</p></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"88 ","pages":"Article 102862"},"PeriodicalIF":2.3,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140890873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
IL-13 protects from C. difficile colitis IL-13 可预防艰难梭菌性结肠炎。
IF 2.3 3区 生物学
Anaerobe Pub Date : 2024-05-01 DOI: 10.1016/j.anaerobe.2024.102860
A.N. Donlan , J.L. Leslie , M.E. Simpson , W.A. Petri , J.E. Allen , W.A. Petri
{"title":"IL-13 protects from C. difficile colitis","authors":"A.N. Donlan ,&nbsp;J.L. Leslie ,&nbsp;M.E. Simpson ,&nbsp;W.A. Petri ,&nbsp;J.E. Allen ,&nbsp;W.A. Petri","doi":"10.1016/j.anaerobe.2024.102860","DOIUrl":"10.1016/j.anaerobe.2024.102860","url":null,"abstract":"<div><h3>Objectives</h3><p><em>Clostridioides difficile</em> infection (CDI) is the leading hospital-acquired infection in North America. We have previously discovered that antibiotic disruption of the gut microbiota decreases intestinal IL-33 and IL-25 and increases susceptibility to CDI. We further found that IL-33 promotes protection through type 2 Innate Lymphoid Cells (ILC2s), which produce IL-13. However, the contribution of IL-13 to disease has never been explored.</p></div><div><h3>Methods</h3><p>We used a validated model of CDI in mice, in which we neutralized via blocking antibodies, or administered recombinant protein, IL-13 to assess the role of this cytokine during infection using weight and clinical scores. Fluorescent activated cell sorting (FACS) was used to characterize myeloid cell population changes in response to IL-13 manipulation.</p></div><div><h3>Results</h3><p>We found that administration of IL-13 protected, and anti-IL-13 exacerbated CDI. Additionally, we observed alterations to the monocyte/macrophage cells following neutralization of IL-13 as early as day three post infection. We also observed elevated accumulation of myeloid cells by day four post-infection following IL-13 neutralization. Neutralization of the decoy receptor, IL-13Rα2, resulted in protection from disease, likely through increased available endogenous IL-13.</p></div><div><h3>Conclusions</h3><p>Our data highlight the protective role of IL-13 in protecting from more severe CDI and the association of poor responses with a dysregulated monocyte-macrophage compartment. These results increase our understanding of type 2 immunity in CDI and may have implications for treating disease in patients.</p></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"88 ","pages":"Article 102860"},"PeriodicalIF":2.3,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S107599642400043X/pdfft?md5=4dedb1eb247b72ce78a9f217b5c3cf29&pid=1-s2.0-S107599642400043X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140855768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Monoclonal antibody-mediated neutralization of Clostridioides difficile toxin does not diminish induction of the protective innate immune response to infection 单克隆抗体介导的艰难梭状芽孢杆菌毒素中和作用不会削弱对感染的先天保护性免疫反应的诱导作用。
IF 2.5 3区 生物学
Anaerobe Pub Date : 2024-05-01 DOI: 10.1016/j.anaerobe.2024.102859
Joshua E. Denny , Md Zahidul Alam , Nontokozo V. Mdluli , Jeffrey R. Maslanka , Linda A. Lieberman , Michael C. Abt
{"title":"Monoclonal antibody-mediated neutralization of Clostridioides difficile toxin does not diminish induction of the protective innate immune response to infection","authors":"Joshua E. Denny ,&nbsp;Md Zahidul Alam ,&nbsp;Nontokozo V. Mdluli ,&nbsp;Jeffrey R. Maslanka ,&nbsp;Linda A. Lieberman ,&nbsp;Michael C. Abt","doi":"10.1016/j.anaerobe.2024.102859","DOIUrl":"10.1016/j.anaerobe.2024.102859","url":null,"abstract":"<div><p><em>Clostridioides difficile</em> infection causes pathology that ranges in severity from diarrhea to pseudomembranous colitis. Toxin A and Toxin B are the two primary virulence factors secreted by <em>C. difficile</em> that drive disease severity. The toxins damage intestinal epithelial cells leading to a loss of barrier integrity and induction of a proinflammatory host response. Monoclonal antibodies (mAbs) that neutralize Toxin A and Toxin B, actoxumab and bezlotoxumab, respectively, significantly reduce disease severity in a murine model of <em>C. difficile</em> infection. However, the impact of toxin neutralization on the induction and quality of the innate immune response following infection is unknown. The goal of this study was to define the quality of the host innate immune response in the context of anti-toxin mAbs therapy. At day 2 post-infection, <em>C. difficile</em>-infected, mAbs-treated mice had significantly less disease compared to isotype-treated mice despite remaining colonized with <em>C. difficile</em>. <em>C. difficile</em>-infected mAbs-treated mice still exhibited marked neutrophil infiltration and induction of a subset of proinflammatory cytokines within the intestinal lamina propria following infection that is comparable to isotype-treated mice. Furthermore, both mAbs and isotype-treated mice had an increase in IL-22-producing ILCs in the intestine following infection. MAbs-treated mice exhibited increased infiltration of eosinophils in the intestinal lamina propria, which has been previously reported to promote a protective host response following <em>C. difficile</em> infection. These findings show that activation of host protective mechanisms remain intact in the context of monoclonal antibody-mediated toxin neutralization.</p></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"88 ","pages":"Article 102859"},"PeriodicalIF":2.5,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1075996424000428/pdfft?md5=770915a065aaf72fe74e7ea8f55c3ff8&pid=1-s2.0-S1075996424000428-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140848350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Theophylline-based control of repA on a Clostridioides difficile plasmid for use in allelic exchange 基于茶碱控制艰难梭菌质粒上的 repA,用于等位基因交换。
IF 2.3 3区 生物学
Anaerobe Pub Date : 2024-04-29 DOI: 10.1016/j.anaerobe.2024.102858
Joshua N. Brehm, Joseph A. Sorg
{"title":"Theophylline-based control of repA on a Clostridioides difficile plasmid for use in allelic exchange","authors":"Joshua N. Brehm,&nbsp;Joseph A. Sorg","doi":"10.1016/j.anaerobe.2024.102858","DOIUrl":"10.1016/j.anaerobe.2024.102858","url":null,"abstract":"<div><p>Historically, mutagenesis in the non-model enteropathogenic bacterium <em>Clostridioides difficile</em> has been challenging. Developing a versatile and reliable method of generating targeted mutations in <em>C. difficile</em> is important to further our understanding of its pathogenesis. Some of the most common targeted mutagenesis systems rely on allelic exchange mediated by either uracil auxotrophy combined with a toxic uracil precursor, a toxin/anti-toxin system, group II introns, or CRISPR/Cas mutagenesis<em>.</em> However, each of these methods suffers from its own issues<em>.</em> Here, we develop and test an allelic exchange strategy which better facilitates screening for integration and selecting for excision than previous systems. This is achieved by controlling plasmid replication with a theophylline-dependent riboswitch cloned upstream of <em>repA,</em> the gene whose product controls plasmid replication. This allows efficient mutant generation, can be performed in a wild-type strain of <em>C. difficile</em>, does not have the off-target effects inherent to group II introns, and alleviates the problem of testing multiple gRNA targets in CRISPR mutagenesis.</p></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"88 ","pages":"Article 102858"},"PeriodicalIF":2.3,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1075996424000416/pdfft?md5=fe6748bed0a9380190a8a8ce0be24021&pid=1-s2.0-S1075996424000416-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140848351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genotypic and phenotypic antimicrobial resistance of Irish Clostridioides difficile isolates, 2022 2022 年爱尔兰艰难梭菌分离株的基因型和表型抗菌药耐药性
IF 2.5 3区 生物学
Anaerobe Pub Date : 2024-04-25 DOI: 10.1016/j.anaerobe.2024.102857
Lieke Brouwer , Anne Carroll , Eleanor McNamara
{"title":"Genotypic and phenotypic antimicrobial resistance of Irish Clostridioides difficile isolates, 2022","authors":"Lieke Brouwer ,&nbsp;Anne Carroll ,&nbsp;Eleanor McNamara","doi":"10.1016/j.anaerobe.2024.102857","DOIUrl":"10.1016/j.anaerobe.2024.102857","url":null,"abstract":"<div><h3>Objectives</h3><p>Infection with <em>Clostridioides difficile</em> usually occurs after antibiotic treatment for other infections and can cause gastro-intestinal disorders of variable severity. <em>C. difficile</em> can be resistant to a wide spectrum of antimicrobials. Detection of antimicrobial resistance (AMR) is important to direct optimal treatment and surveillance of AMR patterns in the overall population. Correlation between genotypic markers and phenotypic AMR is not yet well defined. The aim for this study is to assess whether and to what extent genotypic determinants of AMR correlate with phenotypic resistance.</p></div><div><h3>Methods</h3><p><em>C. difficile</em> isolates (n = 99) were phenotypically characterized for resistance to eight antibiotics using Sensititre plates or E-tests. Their genomes were screened for genetic markers of resistance. Accuracy, sensitivity, specificity, positive and negative predictive values were calculated.</p></div><div><h3>Results</h3><p>We found high rates of resistance (&gt;50 %) to cefoxitin and clindamycin, intermediate rates of resistance (10 %–50 %) to moxifloxacin and tetracycline and low rates of resistance (&lt;10 %) to imipenem, metronidazole, vancomycin, and rifampicin. For moxifloxacin, tetracycline, and clindamycin, we found a good correlation between genotypic and phenotypic AMR, with an overall accuracy of 98 % (95 % CI 93%–100 %), 78 % (95 % CI 68%–86 %) and 86 % (95 % CI 77%–92 %) respectively. For the other five antibiotics, accurate estimates on the correlation could not be made.</p></div><div><h3>Conclusion</h3><p>Our results suggest that for moxifloxacin, tetracycline and clindamycin, phenotypic resistance in <em>C. difficile</em> can be predicted by genetic indicators and used for public health purposes. However, for the other five antibiotics, the model is not accurate and further development is necessary.</p></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"88 ","pages":"Article 102857"},"PeriodicalIF":2.5,"publicationDate":"2024-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1075996424000404/pdfft?md5=03ef5dd0774d6f89f40459ecc7e12e05&pid=1-s2.0-S1075996424000404-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140765300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Novel transcriptional regulator OxtR1 regulates potential ferrodoxin in response to oxygen stress in Treponema denticola 新型转录调控因子 OxtR1 在牙孢子菌对氧胁迫的反应中调控潜在的阿铁毒素
IF 2.3 3区 生物学
Anaerobe Pub Date : 2024-04-16 DOI: 10.1016/j.anaerobe.2024.102852
Yumi Numata , Yuichiro Kikuchi , Toru Sato , Kazuko Okamoto-Shibayama , Yutaro Ando , Yuri Miyai-Murai , Eitoyo Kokubu , Kazuyuki Ishihara
{"title":"Novel transcriptional regulator OxtR1 regulates potential ferrodoxin in response to oxygen stress in Treponema denticola","authors":"Yumi Numata ,&nbsp;Yuichiro Kikuchi ,&nbsp;Toru Sato ,&nbsp;Kazuko Okamoto-Shibayama ,&nbsp;Yutaro Ando ,&nbsp;Yuri Miyai-Murai ,&nbsp;Eitoyo Kokubu ,&nbsp;Kazuyuki Ishihara","doi":"10.1016/j.anaerobe.2024.102852","DOIUrl":"https://doi.org/10.1016/j.anaerobe.2024.102852","url":null,"abstract":"<div><h3>Objective</h3><p><em>Treponema denticola</em> has been strongly implicated in the pathogenesis of chronic periodontitis. Previously, we reported that the potential transcriptional regulator TDE_0259 (<em>oxtR1)</em> is upregulated in the bacteriocin ABC transporter gene-deficient mutant. OxtR1 may regulate genes to adapt to environmental conditions during colonization; however, the exact role of the gene in <em>T. denticola</em> has not been reported. Therefore, we investigated its function using an <em>oxtR1</em>-deficient mutant.</p></div><div><h3>Methods</h3><p>The growth rates of the wild-type and <em>oxtR1</em> mutant were monitored under anaerobic conditions; their antibacterial agent susceptibility and gene expression were assessed using a liquid dilution assay and DNA microarray, respectively. An electrophoretic mobility shift assay was performed to investigate the binding of OxtR1 to promoter regions.</p></div><div><h3>Results</h3><p>The growth rate of the bacterium was accelerated by the inactivation of <em>oxtR1</em>, and the mutant exhibited an increased minimum inhibitory concentration against ofloxacin. We observed a relative increase in the expression of genes associated with potential ferrodoxin (TDE_0260), flavodoxin, ABC transporters, heat-shock proteins, DNA helicase, iron compounds, and lipoproteins in the mutant. OxtR1 expression increased upon oxygen exposure, and <em>oxtR1</em> complementation suppressed the expression of potential ferrodoxin. Our findings also suggested that OxtR1 binds to a potential promoter region of the TDE_0259–260 operon. Moreover, the mutant showed a marginal yet significantly faster growth rate than the wild-type strain under H<sub>2</sub>O<sub>2</sub> exposure.</p></div><div><h3>Conclusion</h3><p>The oxygen-sensing regulator OxtR1 plays a role in regulating the expression of a potential ferrodoxin, which may contribute to the response of <em>T. denticola</em> to oxygen-induced stress.</p></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"87 ","pages":"Article 102852"},"PeriodicalIF":2.3,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140638212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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