Anaerobe最新文献

{"title":"Antimicrobial susceptibility testing of anaerobic bacteria causing bacteremia: A 13-year (2010–2022) retrospective study in a tertiary hospital","authors":"Jorge Ligero-López ,&nbsp;Eduardo Rubio-Mora ,&nbsp;Mario David Ruiz-Bastián ,&nbsp;María Inmaculada Quiles-Melero ,&nbsp;Juana Cacho-Calvo ,&nbsp;Emilio Cendejas-Bueno","doi":"10.1016/j.anaerobe.2023.102803","DOIUrl":"10.1016/j.anaerobe.2023.102803","url":null,"abstract":"<div><p><span><span>Infections from anaerobic microorganisms result from breached mucosal barriers, posing a significant mortality risk. A retrospective study at Hospital Universitario La Paz (Madrid) from 2010 to 2022 analyzed 491 (6.17 %) anaerobic </span>bacteremia cases out of 7956 significant bacteremia cases among 171,833 blood culture requests. </span><span><em>Bacteroides fragilis</em></span> was the most frequently isolated species (28.3 %), followed by <span><em>Clostridium perfringens</em></span> (13.6 %). <em>B. fragilis</em><span> showed good susceptibility to amoxicillin/ clavulanic acid (86 %), piperacillin/tazobactam (86 %), and metronidazole (87.7 %). In general, non-</span><em>fragilis Bacteroides</em><span><span> species showed low susceptibility to penicillin<span> (7 %), amoxicillin (17.5 %), and </span></span>clindamycin (64.9 %). Of our 13 </span><em>non-perfringens Clostridium</em> isolates, four exhibited resistance to penicillin and four showed resistance to clindamycin. <span><em>Lactobacillus</em></span> species were highly susceptible to antibiotics tested. <span><em>Prevotella</em></span><span> spp. showed low susceptibility to penicillin (20 %), amoxicillin (20 %), and clindamycin (40 %). The study contributes valuable data for monitoring and improving anaerobic bacteremia treatment.</span></p></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2023-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138175415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of bezlotoxumab to prevent recurrent Clostridioides difficile infection (CDI) in patients with multiple prior recurrent CDI","authors":"Andrew M. Skinner ,&nbsp;Xing Tan ,&nbsp;Colin Westman ,&nbsp;Thomas Birris ,&nbsp;Emily N. Drwiega ,&nbsp;Michael S. Wang ,&nbsp;Stuart Johnson","doi":"10.1016/j.anaerobe.2023.102788","DOIUrl":"10.1016/j.anaerobe.2023.102788","url":null,"abstract":"<div><p>Among 23 patients with multiply recurrent <em>Clostridiodies difficile</em><span> infection (mrCDI) who received bezlotoxumab<span> at the end of antibiotic treatment a sustained clinical response of 91 % at 30 days and 78 % at 90 days was achieved. Bezlotoxumab administered at the end of antibiotic treatment was effective in patients with mrCDI.</span></span></p></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2023-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71477275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of root canal microbiota profiles of periapical tissue diseases using matrix-assisted laser desorption/ionization time-of-flight mass spectrometer","authors":"Mete Ahlat ,&nbsp;Cumhur Aydin ,&nbsp;Sinem Kaya ,&nbsp;Mehmet Baysallar","doi":"10.1016/j.anaerobe.2023.102791","DOIUrl":"10.1016/j.anaerobe.2023.102791","url":null,"abstract":"<div><h3>Objectives</h3><p>The purpose of this study was to identify microorganisms isolated from various periapical tissue diseases using Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS) and classify them via an unsupervised machine learning approach.</p></div><div><h3>Methods</h3><p>A total of 150 patients with various apical conditions and teeth in need of endodontic retreatment were divided into five groups, including Retreatment, Acute Apical Abscess, Chronic Apical Abscess, Acute Apical Periodontitis, and Chronic Apical Periodontitis. Samples were collected from root canals using paper points after agitating with a #10 K file then microorganisms were identified using MALDI-TOF-MS. Data were analyzed using a hierarchical clustering method. Quadruple clusters and dendrograms were formed according to similarities and dissimilarities.</p></div><div><h3>Results</h3><p>A total of 80 species were identified under six different phyla. The most similar microorganism species were identified <em>''Enterococcus faecalis''</em> between 21 and 23-year-old female cases in Retreatment group; <em>''Lactobacillus rhamnosus''</em> between 20 and 18-year-old male cases in Symptomatic Apical Abscess cases; <em>''Lactobacillus paracasei''</em> between 26 and 40-year-old male cases in Asymptomatic Apical Abscess cases; <em>''Enterococcus faecalis''</em> between 48 and 50-year-old female cases in Symptomatic Apical Periodontitis cases; <em>''Lactobacillus rhamnosus''</em> between 48 and 60-year-old male cases in Asymptomatic Apical Periodontitis cases.</p></div><div><h3>Conclusions</h3><p>MALDI-TOF MS can be considered a fast and high-throughput screening technique for microbial species identification in endodontics. Thus, it will provide valuable data for future research designs regarding periapical tissue diseases. As the MALDI-TOF MS database expands and comprehensive data becomes available, the relationship between microbial profiles and disease progression will become increasingly apparent.</p></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2023-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71477276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Description of Fusibacillus kribbianus gen. nov., sp. nov., a fusiform anaerobe isolated from pig feces","authors":"Lu Bai ,&nbsp;Jayoung Paek ,&nbsp;Yeseul Shin ,&nbsp;Hongik Kim ,&nbsp;Si Hyun Kim ,&nbsp;Jeong Hwan Shin ,&nbsp;Joong-Ki Kook ,&nbsp;Young-Hyo Chang","doi":"10.1016/j.anaerobe.2023.102792","DOIUrl":"10.1016/j.anaerobe.2023.102792","url":null,"abstract":"<div><h3>Objective</h3><p>The family <em>Lachnospiraceae</em> is affiliated with the order Clostridiales and was originally contained within Clostridial cluster XIVa. The members of <em>Lachnospiraceae</em> inhabiting the gut comprise the chemoorganotrophic genera, generating sundry short-chain fatty acids to supply energy to the host, and are considered to be related to obesity and gut health.</p></div><div><h3>Methods</h3><p>The polyphasic taxonomic approach was used to characterize the isolate YH-rum2234^T. A detailed metabolic analysis was conducted to compare the novel isolate with related strains within the family <em>Lachnospiraceae</em>.</p></div><div><h3>Results</h3><p>A fusiform, obligately anaerobic, Gram-stain-negative bacterium, YH-rum2234^T, was isolated from pig feces. Analysis of the 16 S rRNA gene sequence revealed that the similarities between the isolate and the familiarly interrelated strain <em>Lientehia hominis</em> KCTC 25345^T was 94.3 %. The average nucleotide identities and genome-to-genome distances of YH-rum2234^T and its closely related strains were below 85.5 % and 32.5 %, respectively. The G + C content of the genomic DNA was 49.2 mol%. The main fatty acids were C_16:0, C_14:0, and C_14:0 DMA. The major polar lipids were aminophospholipids. The cell wall did not contain the peptidoglycan <em>meso</em>-diaminopimelic acid.</p></div><div><h3>Conclusion</h3><p>Given the chemotaxonomic, phenotypic, and phylogenetic properties, YH-rum2234^T (=KCTC 25710^T = DSMZ 116041^T) represents a new genus and species in the family <em>Lachnospiraceae</em>. <em>Fusibacillus kribbianus</em> gen. nov., sp. nov. is the proposed name.</p></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2023-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1075996423001014/pdfft?md5=82aac80a5f08a2ec78d355d908551856&pid=1-s2.0-S1075996423001014-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71477274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth assessment of mixed cultures of probiotics and common pathogens","authors":"Mansa Fredua-Agyeman ,&nbsp;Paul Stapleton ,&nbsp;Simon Gaisford","doi":"10.1016/j.anaerobe.2023.102790","DOIUrl":"10.1016/j.anaerobe.2023.102790","url":null,"abstract":"<div><h3>Objectives</h3><p><span>In this work, an isothermal microcalorimeter was applied to investigate the antipathogenic activity of three probiotics (</span><span><em>Lactobacillus acidophilus</em></span>, <span><em>Bifidobacterium lactis</em></span> and <span><em>Bifidobacterium bifidum</em></span>) against <span><em>Pseudomonas aeruginosa</em></span>, <span><em>Staphylococcus aureus</em></span> and <em>Escherichia coli</em><span> using the probiotics in mixed culture with the pathogenic microorganisms.</span></p></div><div><h3>Methods</h3><p><span>A microcalorimeter was used to monitor the growth of the microorganisms as pure cultures and as co-cultures at 37 °C. Relative growths of the probiotics and pathogenic species were determined after microcalorimetric measurements by serial dilution and plate incubation. Relative growth of mixed cultures of </span><em>E. coli</em> with <em>L. acidophilus</em> or <em>B. lactis</em><span> was also determined by traditional plate growth assay for 5.5 h.</span></p></div><div><h3>Results</h3><p>The results showed growth profiles of the microorganisms that were characteristic and showed different lag and peak times for the species. The pathogenic species grew faster than the probiotic species. In the co-cultures, the growth profile of both pathogenic species and probiotics could be identified with the microcalorimeter. Although the pathogenic species grew faster, at the end of the assay, the results showed that the pathogenic species were inhibited in growth by the probiotics as no viable growth of the pathogenic species was detected whereas 10⁷–10⁸ CFU/mL of the probiotics were enumerated after the microcalorimetric assay. Using the traditional plate assay, the data confirmed co-growth of the probiotics and <em>E. coli</em> although cell numbers of <em>E. coli</em> were higher than the probiotics during 5.5 h of co-culture incubation when both were inoculated at 10⁶ CFU/mL.</p></div><div><h3>Conclusion</h3><p>The results demonstrate the antipathogenic effects of probiotics and highlights the potential of microcalorimetry in live mixed culture assays and its limitation.</p></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2023-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136128349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral teicoplanin administration suppresses recurrence of Clostridioides difficile infection: Proof of concept","authors":"Yoko Tanaka ,&nbsp;Sho Tashiro ,&nbsp;Shintaro Ikegami,&nbsp;Yuki Enoki,&nbsp;Kazuaki Taguchi,&nbsp;Kazuaki Matsumoto","doi":"10.1016/j.anaerobe.2023.102789","DOIUrl":"10.1016/j.anaerobe.2023.102789","url":null,"abstract":"<div><h3>Objectives</h3><p><span>Teicoplanin is a potential antimicrobial candidate for </span><span><em>Clostridioides difficile</em></span><span> infection (CDI) treatment. However, the therapeutic potential of teicoplanin against severe CDI has not been clinically proven. In the present study, we investigated the efficacy of oral teicoplanin administration against severe CDI and the recurrence of severe CDI after teicoplanin treatment in a mouse model.</span></p></div><div><h3>Methods</h3><p>A lethal CDI mouse model was established by colonizing the mice with <em>C. difficile</em><span> ATCC® 43255; they were orally administered teicoplanin (128 mg/kg/d) or vancomycin (160 mg/kg/d) for 10 d, 24 h after </span><em>C. difficile</em><span> spore challenge, and physiological and biological responses were monitored for 20 d after the initial antibiotic treatment. We also performed the </span><em>in vitro</em> time-kill assay and determined minimum inhibitory concentration (MIC), post-antibiotic effect, and toxin production with antibiotic exposure.</p></div><div><h3>Results</h3><p><span>The therapeutic response (survival rates, body weight change, clinical sickness score grading, </span><em>C. difficile</em> load, and toxin titer in feces) of oral teicoplanin administration was comparable to that of oral vancomycin administration in the lethal CDI mouse model. Moreover, teicoplanin treatment suppressed the re-onset of diarrhea and re-increase in toxin titer 10 d after treatment compared with that by vancomycin treatment. In <em>in vitro</em><span> experiments, teicoplanin exhibited time-dependent antibacterial activity and possessed lower MIC and longer post-antibiotic effect than vancomycin against </span><em>C. difficile</em>. <em>C. difficile</em> toxin production was numerically lower with teicoplanin exposure than with vancomycin exposure.</p></div><div><h3>Conclusions</h3><p>The results obtained from the present basic experiments could suggest that teicoplanin is a potential antibiotic for the treatment of severe CDI with recurrence-prevention activity.</p></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2023-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50160449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical significance of Bacteroides fragilis as a potential prognostic factor in colorectal cancer","authors":"Yuriko Matsumiya ,&nbsp;Mitsukuni Suenaga ,&nbsp;Toshiaki Ishikawa ,&nbsp;Toshifumi Kudo ,&nbsp;Tsuyoshi Nakagawa ,&nbsp;Kentaro Okamoto ,&nbsp;Masanori Tokunaga ,&nbsp;Claudia Hurtado ,&nbsp;Yuki Yamada ,&nbsp;Kentaro Oka ,&nbsp;Motomichi Takahashi ,&nbsp;Luis Francisco Lopez Kostner ,&nbsp;Miguel Luis O'Ryan Gallardo ,&nbsp;Hiroyuki Uetake ,&nbsp;Yusuke Kinugasa","doi":"10.1016/j.anaerobe.2023.102784","DOIUrl":"10.1016/j.anaerobe.2023.102784","url":null,"abstract":"<div><h3>Introduction</h3><p><span><em>Bacteroides fragilis</em></span> (<em>B. fragilis</em>) is considered to act in an anti-inflammatory manner on the intestinal tract. On the contrary, enterotoxigenic <em>B. fragilis</em> (ETBF), a subtype of <em>B. fragilis</em><span>, produces an enterotoxin (BFT; </span><em>B. fragilis</em><span> toxin), leading to asymptomatic chronic infections and colonic tumor formation. However, the impact of </span><em>B. fragilis</em><span> and ETBF on the clinical outcome of colorectal cancer (CRC) remains unclear. We aim to assess whether their presence affects the outcome in patients with CRC after curative resection.</span></p></div><div><h3>Methods</h3><p>We obtained 197 pairs of matched formalin-fixed paraffin-embedded samples from cancerous and adjacent non-cancerous tissues of patients with pathological stage (pstage) II and III CRC after curative resection. The presence of <em>B. fragilis</em> and ETBF were estimated using real-time polymerase chain reaction, and recurrence-free survival (RFS) and overall survival (OS) of the patients were analyzed.</p></div><div><h3>Results</h3><p><span>16 S rRNA for </span><em>B. fragilis</em> and <em>bft</em> DNA were detected in 120 (60.9%) and 12 (6.1%) of the 197 patients, respectively. <em>B. fragilis</em>-positive patients had better RFS than <em>B. fragilis</em>-negative patients, although that was not statistically significant. In subgroup analysis, better outcomes on RFS were observed in the presence of <em>B. fragilis</em> in pstage II and left-sided CRC. The association of <em>B. fragilis</em> positivity on OS was accentuated in the depth of T4 subgroup. No significant differences were observed in RFS and OS between ETBF and non-toxigenic <em>B. fragilis</em>.</p></div><div><h3>Conclusions</h3><p>Our findings suggest that the presence of <em>B. fragilis</em> is associated with better outcomes in patients with pstage II and III CRC after curative resection.</p></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2023-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41095630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Granular activated carbon enhances the anaerobic digestion of solid and liquid fractions of swine effluent at different mesophilic temperatures","authors":"Miriam Orrantia ,&nbsp;Edna R. Meza-Escalante ,&nbsp;Vianey A. Burboa-Charis ,&nbsp;Refugio B. García-Reyes ,&nbsp;Marina M. Atilano-Camino ,&nbsp;Denisse Serrano-Palacios ,&nbsp;Luis A. Leyva ,&nbsp;Yair A. Del Angel ,&nbsp;Luis H. Alvarez","doi":"10.1016/j.anaerobe.2023.102782","DOIUrl":"10.1016/j.anaerobe.2023.102782","url":null,"abstract":"<div><h3>Objectives</h3><p><span>This study evaluated the effect of particle size and dosage of granular activated carbon (GAC) on methane production from the </span>anaerobic digestion of raw effluent (RE) of swine wastewater, and the solid (SF) and liquid (LF) fractions. The effect of temperature using the selected size and dosage of GAC was also evaluated.</p></div><div><h3>Methods</h3><p>60 mL of swine wastewater were inoculated with anaerobic granular sludge and GAC at different dosages and particle size. The cultures were incubated at different temperatures at 130 rpm. The kinetic parameters from experimental data were obtained using the Gompertz model.</p></div><div><h3>Results</h3><p>The cultures with the LF and GAC (75–150 μm, 15 g/L) increased 1.87-fold the methane production compared to the control without GAC. The GAC at 75–150 μm showed lower lag phases and higher R_max<span> than the cultures with GAC at 590–600 μm. The cumulative methane production at 45 °C with the RE + GAC was 7.4-fold higher than the control. Moreover, methane production at 45 °C significantly increased with the cultures LF + GAC (6.0-fold) and SF + GAC (2.0-fold). The highest production of volatile fatty acids and ammonium was obtained at 45 °C regardless of the substrate and the addition of GAC contributed to a higher extent than the cultures lacking GAC. In most cases, the kinetic parameters at 30 °C and 37 °C were also higher with GAC.</span></p></div><div><h3>Conclusions</h3><p>GAC contributed to improving the fermentative and methanogenesis stages during the anaerobic digestion of fractions, evidenced by an improvement in the kinetic parameters.</p></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10288792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autolysin as a fibronectin receptor on the cell surface of Clostridium perfringens","authors":"Riyo Aono ,&nbsp;Shogo Emi ,&nbsp;Kanako Okabe-Watanabe ,&nbsp;Hirofumi Nariya ,&nbsp;Nozomu Matsunaga ,&nbsp;Yasuo Hitsumoto ,&nbsp;Seiichi Katayama","doi":"10.1016/j.anaerobe.2023.102769","DOIUrl":"10.1016/j.anaerobe.2023.102769","url":null,"abstract":"<div><h3>Objective</h3><p><span><em>Clostridium perfringens</em></span><span> causes food poisoning and gas gangrene, a serious wound-associated infection. </span><em>C. perfringens</em><span> cells adhere to collagen via fibronectin (Fn). We thought that </span><em>C. perfringens</em><span> cells have some kind of Fn receptor. We investigated whether the peptidoglycan<span> hydrolase of </span></span><em>C. perfringens</em>, <em>i.e.,</em><span> autolysin (Acp), is implicated in Fn binding to </span><em>C. perfringens</em> cells.</p></div><div><h3>Methods</h3><p>This study used recombinant Acp fragments, human Fn and knockout mutants (<em>C. perfringens</em> 13 <em>acp::erm</em> and HN13 <em>ΔfbpC ΔfbpD</em><span><span>). Ligand blotting, Western blotting analysis, and complementation tests were performed. The Fn-binding activity of each mutant was evaluated by </span>ELISA.</span></p></div><div><h3>Results</h3><p><span><span>From an Fn-binding assay using recombinant Acp fragments, Fn was found to bind to the catalytic domain of Acp. In </span>mutant cells lacking Acp, Fn binding was significantly decreased, but was restored by the complementation of the </span><em>acp</em> gene. There are three known kinds of Fn-binding proteins in <em>C. perfringens</em>: FbpC, FbpD, and glyceraldehyde-3-phosphate dehydrogenase. We found no difference in Fn-binding activity between the mutant cells lacking both FbpC and FbpD (SAK3 cells) and the wild-type cells, indicating that these Fn-binding proteins are not involved in Fn binding to <em>C. perfringens</em> cells.</p></div><div><h3>Conclusions</h3><p>We found that the Acp is an Fn-binding protein that acts as an Fn receptor on the surface of <em>C. perfringens</em> cells.</p></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10022538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of microbiome-based therapeutics for the reduction and prevention of antimicrobial-resistant organism colonization","authors":"Armaghan-e-Rehman Mansoor,&nbsp;Caroline A. O'Neil,&nbsp;Jennie H. Kwon","doi":"10.1016/j.anaerobe.2023.102772","DOIUrl":"10.1016/j.anaerobe.2023.102772","url":null,"abstract":"<div><p><span><span>The gut is host to a diverse array of microbiota that constitute a complex ecological system crucial to </span>human physiology<span>. Disruptors to the normal host microbiota, such as antimicrobials, can cause a loss of species diversity in the gut, reducing its ability to resist colonization by invading pathogens<span><span> and potentially leading to colonization with antimicrobial resistant organisms (AROs). ARO negatively impact gut health by disrupting the usual heterogeneity of gut microbiota and have the potential to cause </span>systemic disease<span>. In recent years, fecal microbiota transplantation (FMT) has been increasingly explored in the management of specific disease states such as </span></span></span></span><span><em>Clostridioides difficile</em></span> infection (CDI). Promising data from management of CDI has led to considerable interest in understanding the role of therapeutics to restore the gut microbiota to a healthy state. This review aims to discuss key studies that highlight the current landscape, and explore existing clinical evidence, for the use of FMT and microbiome-based therapeutics in combating intestinal colonization with ARO. We also explore potential future directions of such therapeutics and discuss unaddressed needs in this field that merit further investigation.</p></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10073026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}