Anaerobe最新文献

{"title":"Deletion of rgaS reduces virulence in a mouse model of Clostridioides difficile infection","authors":"Terry W. Bilverstone ,&nbsp;Morgan E. Simpson ,&nbsp;Nicholas Hagspiel ,&nbsp;William A. Petri ,&nbsp;Sarah A. Kuehne ,&nbsp;Nigel P. Minton","doi":"10.1016/j.anaerobe.2025.102988","DOIUrl":"10.1016/j.anaerobe.2025.102988","url":null,"abstract":"<div><div>Two-component system signalling is involved in several processes relevant to <em>Clostridioides difficile</em> virulence. We demonstrate that deletion of the orphan histidine kinase <em>rgaS</em> (R20291_0503), reduces detectable toxin in the supernatant of R20291 and impairs virulence in a mouse model of infection. These data suggest that RgaS regulates virulence capabilities in hypervirulent <em>C. difficile</em>.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"95 ","pages":"Article 102988"},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144820425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High prevalence and clonality of Clostridioides difficile isolates from cryptic clades in household composters and small mammals","authors":"Klemen Trsinar ,&nbsp;Sabina Mlakar ,&nbsp;Franc Janzekovic ,&nbsp;Maja Rupnik ,&nbsp;Sandra Janezic","doi":"10.1016/j.anaerobe.2025.102990","DOIUrl":"10.1016/j.anaerobe.2025.102990","url":null,"abstract":"<div><h3>Objective</h3><div>We aimed to investigate the prevalence, genotypic diversity, and clonality of <em>Clostridioides difficile</em> strains from household composters and faeces of small mammals captured nearby.</div></div><div><h3>Methods</h3><div>Compost piles from three locations were sampled, with three compost samples collected from each bin. Additionally, 5–6 small mammals were trapped around each compost pile. <em>C. difficile</em> was isolated from faecal and compost samples and characterized with PCR ribotyping, toxinotyping, and genome sequencing.</div></div><div><h3>Results</h3><div><em>C. difficile</em> was detected in all nine compost samples, while only two (12.5 %) of 16 small mammal faecal samples tested positive. Ten PCR ribotypes (RTs) were identified, with only one, RT014/020, being toxigenic. The remaining nine RTs belonged to two cryptic clades, C-II and C-III. Some of the isolates from cryptic clades i.e. RT SLO 308 carried a divergent <em>tcd</em>A gene and yielded weakly positive results with <em>C. diff</em> Quik Chek Complete immunoassay. Clonal <em>C. difficile</em> isolates of RT SLO 308 were found at the same site in both a compost sample and a small mammal, suggesting potential transmission. Clonality was also observed among isolates from different compost samples within a single pile.</div></div><div><h3>Conclusions</h3><div>While our study could not establish the exact direction of <em>C. difficile</em> transmission between compost and mice, it highlights the role of small mammals within the One Health framework of <em>C. difficile</em> transmission pathways.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"95 ","pages":"Article 102990"},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolism of Lactobacillus and Gardnerella vaginalis in vaginal defined media","authors":"Victoria Horrocks ,&nbsp;Charlotte K. Hind ,&nbsp;J. Mark Sutton ,&nbsp;Rachel M. Tribe ,&nbsp;A. James Mason","doi":"10.1016/j.anaerobe.2025.102991","DOIUrl":"10.1016/j.anaerobe.2025.102991","url":null,"abstract":"<div><h3>Objectives</h3><div>This study evaluates how well a vaginal defined medium (VDM) replicates the i<em>n vivo</em> metabolic behaviour of key vaginal microbiota members - <em>Lactobacillus crispatus</em>, <em>L. jensenii</em>, and diverse <em>Gardnerella vaginalis</em> isolates - compared to brain heart infusion (BHI) medium.</div></div><div><h3>Methods</h3><div>We used ¹H NMR spectroscopy to characterise metabolic profiles during <em>in vitro</em> growth of <em>Lactobacillus</em> and <em>Gardnerella</em> species in VDM and BHI. Differences in metabolite production, growth, acidification, and carbohydrate utilisation were assessed.</div></div><div><h3>Results</h3><div>Both <em>L.</em> <em>crispatus</em> and <em>L. jensenii</em> grow well in VDM, produce substantially more lactate than in BHI, and acidify the culture more strongly - better reflecting the low pH environment of <em>Lactobacillus</em>-dominant vaginal microbiota. In contrast, <em>G. vaginalis</em> grows less robustly in VDM than in BHI, though key metabolic traits such as the Bifidobacterium shunt and mixed acid fermentation (evidenced by formate production) are preserved. Notably, neither genus consume available glucose, yet still ferment carbohydrates, suggesting a metabolic preference for glycogen over glucose. Evidence of glucose release further indicates glycogen breakdown in culture.</div></div><div><h3>Conclusions</h3><div>VDM more accurately models the metabolic activity and environmental effects of vaginal <em>Lactobacillus</em> species than BHI, particularly in terms of acidification and lactate production. Although <em>G. vaginalis</em> growth is limited in VDM, its characteristic metabolic pathways remain evident. These findings underscore the value of VDM in modelling key metabolic features of the vaginal microbiota, especially under conditions where <em>Lactobacillus</em> dominate or <em>Gardnerella</em> is prevalent.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"95 ","pages":"Article 102991"},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144903810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Odoribacter splanchnicus bacteremia: a rare condition associated with intestinal disease","authors":"Hiroshi Umemura ,&nbsp;Hirokazu Kobayashi ,&nbsp;Yumiko Tanimichi ,&nbsp;Hiroyuki Nishiyama ,&nbsp;Natsumi Ikumi ,&nbsp;Masaki Nakajima ,&nbsp;Sachio Tsuchida ,&nbsp;Tomohiro Nakayama","doi":"10.1016/j.anaerobe.2025.102994","DOIUrl":"10.1016/j.anaerobe.2025.102994","url":null,"abstract":"<div><div><em>Odoribacter splanchnicus</em> is an anaerobe that normally inhabits the human intestine and rarely causes infections in humans. In recent years, however, three cases of <em>O. splanchnicus</em> bacteremia have been reported. We retrospectively searched our laboratory information system and detected five cases of <em>O. splanchnicus</em> bacteremia in 2014–2024. All cases of <em>O. splanchnicus</em> bacteremia reported to date have been associated with intestinal infection, but our five cases included one associated with decubitus ulcer infection. Antimicrobial susceptibility testing results were available in four of our five cases. <em>O. splanchnicus</em> showed susceptibility to beta-lactams and metronidazole. However, one isolate was resistant to clindamycin, and another showed intermediate susceptibility to levofloxacin. Among our five cases and the three previously reported cases, no patient was using immunosuppressive agents or had immunocompromising diseases except for one case with malignancy. While <em>O. splanchnicus</em> bacteremia is certainly rare, more cases may be found by searching the laboratory information system as in this investigation. Further accumulation of cases will help to elucidate the pathogenesis of infections caused by <em>O. splanchnicus</em>, which is endemic in the gastrointestinal tract, and will reveal more information on antimicrobial susceptibility.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"95 ","pages":"Article 102994"},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145008067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization of Clostridioides difficile isolated from older adults during prospective population-based active surveillance at nine hospitals in Tokyo, Japan, 2018-2020.","authors":"Zhenghui Li, Ashlesh K Murthy, C Hal Jones, Kwok Lee, Urvi Rajyaguru, Deepika Athinarayanan, Lubomira Andrew, Richard V Goering, Shuhei Ito, Elisa Gonzalez, Pingping Zhang, Paul A Liberator, Warren V Kalina, Michael W Pride, Frederick J Angulo, Jennifer C Moïsi, Kazuhiro Tateda","doi":"10.1016/j.anaerobe.2025.102985","DOIUrl":"10.1016/j.anaerobe.2025.102985","url":null,"abstract":"<p><strong>Background: </strong>To characterize Clostridioides difficile isolates identified during a prospective multi-hospital population-based study of C. difficile infection (CDI).</p><p><strong>Methods: </strong>Between December 2018 and March 2020, inpatients ≥50 years of age with new-onset diarrhea in nine Tokyo hospitals were investigated for CDI. Stool specimens were screened by C. DIFF QUIK CHEK COMPLETE® and glutamate dehydrogenase (GDH) positive stools tested by Xpert® C. difficile/Epi PCR assay. PCR positive stools were tested by cytotoxicity neutralization assay to determine presence of functional toxin. GDH positive stools were also anaerobically cultured and whole genome sequences of C. difficile isolates were acquired.</p><p><strong>Results: </strong>Toxigenic C. difficile were isolated from 64 patients with CDI; 22 were RT018/356, 9 were RT369, 4 each were RT106 and RT002, 15 were other RTs, and 10 had an unknown RT. Four isolates were positive for binary toxin: one isolate each of RT027, RT078/126, RT080, and one unknown. In terms of the pathogenicity locus profile, 55 were tcdA+/tcdB+ and 9 were tcdA-/tcdB+. In terms of ST, 23 were ST17, nine were ST81, 31 were other STs, and one had a novel ST.</p><p><strong>Conclusions: </strong>Consistent with prior studies, C. difficile isolates from patients with CDI represented diverse ribotypes with approximately one third of isolates RT018/356. Of note, one isolate each of RT027 and RT078/126 were identified, indicating these hypervirulent strains are present in hospitals in Japan. Public health interventions are needed to reduce the CDI burden in Japan.</p>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":" ","pages":"102985"},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144666880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Desulfovibrio desulfuricans bacteraemia and associated liver abscess: Case report and literature review","authors":"Katharine Xiwen Huang ,&nbsp;Justin Deane Jackson","doi":"10.1016/j.anaerobe.2025.102973","DOIUrl":"10.1016/j.anaerobe.2025.102973","url":null,"abstract":"<div><div>We present a rare case of <em>Desulfovibrio desulfuricans</em> bacteraemia and associated liver abscess. A 72-year-old Caucasian woman presented with an acute history of fevers, rigors and abdominal pain. She was managed empirically with ceftriaxone and metronidazole and required 24 hours of inotropic support due to hypotension from sepsis. A computed tomography scan of her abdomen demonstrated a 6cm liver abscess in maximal diameter. On day seven of her admission, blood cultures flagged an anaerobic gram-negative bacillus, which was later identified by MALDI-TOF MS as <em>Desulfovibrio desulfuricans</em>. The isolate was susceptible to amoxicillin-clavulanic acid, ertapenem, moxifloxacin, clindamycin and metronidazole but resistant to piperacillin-tazobactam. Despite percutaneous drainage on day two of admission, her fevers persisted for three weeks. On day nine of admission, intravenous ceftriaxone and metronidazole were switched to amoxicillin-clavulanic acid, which was administered for a total of six weeks, resulting in clinical resolution. Our case highlights <em>Desulfovibrio desulfuricans</em> as an uncommon cause of liver abscess and one which is important to consider if culturing an anaerobic, spiral gram-negative bacilli. Empirical antibiotics need to be carefully considered as there may be resistance to beta-lactam antibiotics including piperacillin-tazobactam.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"95 ","pages":"Article 102973"},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144198083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vivo efficacy of fidaxomicin against rpoB mutant Clostridioides difficile infection","authors":"Mai Thu Hoai ,&nbsp;Yutaro Hitomi ,&nbsp;Tsutomu Fujii ,&nbsp;Yoshitomo Morinaga","doi":"10.1016/j.anaerobe.2025.102992","DOIUrl":"10.1016/j.anaerobe.2025.102992","url":null,"abstract":"<div><h3>Objectives</h3><div><em>Clostridioides difficile</em> infection (CDI) is a well-known healthcare-associated diarrheal disease. Fidaxomicin, a key antibiotic used to treat CDI, targets bacterial RNA polymerase. However, some clinical isolates have mutations in <em>rpoB</em>, which reduces their susceptibility to this antibiotic. In this study, the effects of <em>rpoB</em> mutations on the virulence of <em>C. difficile</em> and efficacy of fidaxomicin against CDI were evaluated <em>in vivo</em>.</div></div><div><h3>Methods</h3><div>An <em>rpoB</em> mutant strain (<em>C. difficile</em> G1073R-2024) with reduced fidaxomicin susceptibility was generated through spontaneous induction in a murine CDI model from the parental strain <em>C. difficile</em> VPI 10463. The virulence and therapeutic responses of the mutant strain were compared with those of the parental strain using a CDI model, including survival rate, body weight changes, clinical scores, and bacterial loads in feces.</div></div><div><h3>Results</h3><div><em>C. difficile</em> G1073R-2024 had an amino acid alteration in Gln1073Arg and the minimum inhibitory concentration of fidaxomicin was 128 μg/mL. <em>In vivo</em> virulence was not significantly different between strains. Fidaxomicin treatment resulted in 100 % survival rates and a comparable reduction in the bacterial load for both strains.</div></div><div><h3>Conclusions</h3><div>Fidaxomicin was effective against CDI caused by the <em>rpoB</em> mutant strain. The emergence of such mutations highlights the need for ongoing surveillance of drug resistance trends in clinical settings.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"95 ","pages":"Article 102992"},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145005828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel anaerobic taxa and taxonomic revisions published in 2024, and requirements for the validation process to establish taxon names with standing in the literature","authors":"Paul A. Lawson ,&nbsp;Samuel Miller","doi":"10.1016/j.anaerobe.2025.102989","DOIUrl":"10.1016/j.anaerobe.2025.102989","url":null,"abstract":"","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"95 ","pages":"Article 102989"},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144811531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clostridioides (Clostridium) difficile in children and adolescents in the community in Cambodia","authors":"Lengsea Eng ,&nbsp;Paul Turner ,&nbsp;Kefyalew Addis Alene ,&nbsp;Deirdre A. Collins ,&nbsp;Su-Chen Lim ,&nbsp;Pisey Tan ,&nbsp;Sona Soeng ,&nbsp;Dylorng Hun ,&nbsp;Sotera Yohn ,&nbsp;Sarim Vong ,&nbsp;Archie C.A. Clements ,&nbsp;Thomas V. Riley","doi":"10.1016/j.anaerobe.2025.102982","DOIUrl":"10.1016/j.anaerobe.2025.102982","url":null,"abstract":"<div><h3>Background</h3><div><em>Clostridioides (Clostridium) difficile</em> transmission between community and healthcare settings has been increasingly reported. We aimed to identify the prevalence and molecular epidemiology of <em>C. difficile</em> colonising adolescents and non-hospitalised children in Cambodia.</div></div><div><h3>Methods</h3><div>Stool samples were collected from 266 students at the University of Health Sciences (UHS) in Phnom Penh, between July and August 2022, and 246 children ≥1 year old visiting the outpatient department (OPD) at Angkor Hospital for Children in Siem Reap, between January and August 2022. <em>C. difficile</em> culture, toxin gene detection and PCR ribotyping were performed.</div></div><div><h3>Results</h3><div>Overall, <em>C. difficile</em> was recovered from 07/266 specimens (2.6 %) from UHS students and 59/246 specimens (23.9 %) from OPD children. The overall prevalence of <em>C. difficile</em> in children peaked in April, and of toxigenic strains peaked in August. Children with loose stools were less likely to be colonised by <em>C. difficile</em> (COR = 0.05, 95 %CI: 0.008–0.32) than children with normal stools. UHS students were colonised only by toxigenic <em>C. difficile.</em> Of the 66 isolates, 36 % (24/66) were toxigenic: <em>C. difficile</em> ribotype (RT) 017 was the most predominant, followed by RTs 012, 046, 056, QX709, 001, 014/020 and QX710. Non-toxigenic strains accounted for 35 RTs including 32 novel RTs that had not been isolated previously.</div></div><div><h3>Conclusions</h3><div>The findings of only toxigenic strains in adolescents and the high prevalence of <em>C. difficile</em> in OPD children suggest exposure to <em>C. difficile</em> within the community; thus, appropriate interventions may be needed. Genotypic identification of diverse <em>C. difficile</em> is important for molecular epidemiological purposes.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"94 ","pages":"Article 102982"},"PeriodicalIF":2.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144564307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative evaluation of indirect-ELISAs based on native antigens of Clostridium chauvoei for the detection of blackleg-specific antibodies in cattle","authors":"Mevaliya Amitkumar ,&nbsp;Awadhesh Prajapati ,&nbsp;Suresh Bindu ,&nbsp;Sripada Sairam ,&nbsp;Anand Shirisha ,&nbsp;Mandrira Ramakrishna Namrutha ,&nbsp;Roopa Anandamurthy Hemanth ,&nbsp;Revanaiah Yogisharadhya ,&nbsp;Mohammed Mudassar Chanda ,&nbsp;Sathish Bhadravati Shivachandra","doi":"10.1016/j.anaerobe.2025.102975","DOIUrl":"10.1016/j.anaerobe.2025.102975","url":null,"abstract":"<div><h3>Objective</h3><div>Blackleg, also known as black quarter (BQ), is an acute and lethal infection in bovines and caused by the anaerobic bacterium- <em>Clostridium chauvoei.</em> A killed vaccine is available for disease control, and evaluating the immune response to the vaccine or natural infection in susceptible animals is crucial for implementing effective vaccination strategies in endemic regions. This study focused on the development and comparative evaluation of indirect ELISAs based on native antigens (whole cell and flagellar) from <em>C</em>. <em>chauvoei</em> to detect blackleg-specific antibodies in serum samples collected from cattle.</div></div><div><h3>Methods</h3><div>Whole cell and flagellar antigens were extracted conventionally from <em>C</em>. <em>chauvoei</em> strain NIVEDIBQ1, and optimized indirect ELISAs were compared with the indirect hemagglutination assay (IHA). These assays were used to screen 810 serum samples collected from Andhra Pradesh, India, where blackleg is endemic.</div></div><div><h3>Results</h3><div>Optimum antigen concentrations (250 ng of whole cell antigen and 300 ng of flagella), serum dilution (1:100), and conjugate dilution (1: 10,000) were determined using the checkerboard titration method. The optimized assays reliably detected <em>C</em>. <em>chauvoei</em>-specific antibodies at serum dilutions as low as 1:1600 for whole cell antigens, and 1: 800 for flagellar antigens without any cross-reactions. A comparison between IHA and indirect ELISAs using Cohen's Kappa analysis indicated that the two assays were equally effective. A diagnostic sensitivity (DSn) of 96.0 % and 95.0 %, and diagnostic specificity (DSp) of 95.0 % and 97.0 %, with cut-off criteria of &gt;20.7632 and 18.579, for whole cell and flagellar antigens, respectively, was observed after ROC analysis. The observed seropositivity rates in Andhra Pradesh were 57 % (462) and 40.9 % (332) for whole cell and flagellar antigen based indirect ELISAs, respectively.</div></div><div><h3>Conclusion</h3><div>This study demonstrated the potential application of native antigen-based indirect ELISAs for monitoring blackleg-specific antibodies in cattle from endemic regions.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"94 ","pages":"Article 102975"},"PeriodicalIF":2.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144198082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}