Anaerobe最新文献_第6页

Cultivation of anaerobic bacteria: Foundations and principles 厌氧细菌的培养：基础和原理

IF 2.5 3区生物学

Anaerobe Pub Date : 2025-03-24 DOI: 10.1016/j.anaerobe.2025.102951

Paul A. Lawson, Ralph S. Tanner

引用次数: 0

Antimicrobial resistance profiles of Clostridium species isolated from post-traumatic infections in a Costa Rican hospital 哥斯达黎加一家医院创伤后感染中分离的梭状芽孢杆菌的抗微生物药物耐药性谱

IF 2.5 3区生物学

Anaerobe Pub Date : 2025-03-24 DOI: 10.1016/j.anaerobe.2025.102957

Mariela Alvarado-Rodríguez , Carlos Quesada-Gómez

引用次数: 0

Obituary of Mike Cox (1945–2024) 迈克·考克斯讣告（1945-2024）

IF 2.5 3区生物学

Anaerobe Pub Date : 2025-03-22 DOI: 10.1016/j.anaerobe.2025.102956

Elisabeth Nagy, Ellen Jo Baron

引用次数: 0

Molecular clock complexities of Clostridioides difficile 艰难梭菌的分子钟复杂性。

IF 2.5 3区生物学

Anaerobe Pub Date : 2025-03-19 DOI: 10.1016/j.anaerobe.2025.102953

Jon J. Vernon , David W. Eyre , Mark H. Wilcox , Jane Freeman

{"title":"Molecular clock complexities of Clostridioides difficile","authors":"Jon J. Vernon , David W. Eyre , Mark H. Wilcox , Jane Freeman","doi":"10.1016/j.anaerobe.2025.102953","DOIUrl":"10.1016/j.anaerobe.2025.102953","url":null,"abstract":"<div><h3>Objectives</h3><div>Reconstruct the phylogenetic status of a collection of historical <em>Clostridioides difficile</em> isolates and evaluate the congruence of their evolutionary trajectories with established molecular clock models.</div></div><div><h3>Methods</h3><div>Phylogenetic analysis was performed on Illumina sequence reads from previously analysed historic <em>C. difficile</em> isolates (1980–86; <em>n</em> = 75) demonstrating multiple antimicrobial resistances. Data was grouped by ribotype (RT), including comparators from European surveillance (2012–13) and phylogenetic studies (1985–2010). Reads were mapped to CD630/CD196 reference genomes and compared using recombination-adjusted maximum likelihood trees. Prediction intervals for expected SNP differences by age were calculated using a Poisson distribution and molecular clock estimates (0.74 SNPs per genome/per year). Root-to-tip analysis was performed to determine the date of most common recent ancestor of genomes sharing a ribotype.</div></div><div><h3>Results</h3><div>Moxifloxacin-resistant (>16 mg/L) RT027 isolate JV67 (1986) was two SNPs distinct from a 2006 genome, fewer than the expected lower estimate (4.4 SNPs) under current molecular clock calculations; (<em>p</em> = 3.93x10<sup>−5</sup>). For isolate JV02 (1981), the 13 SNP divergence from a 2008 isolate was consistent with expectations (5.9 SNPs; <em>p</em> = 0.07). JV73 (1983) demonstrated an 8 SNP difference, which although above the expected lower limit (5.5 SNPs), was outside the 95 % prediction interval; (<em>p</em> = 4.51x10<sup>−3</sup>). Only sixty-nine percent of historical genomes fit within the prediction interval for the number of SNPs expected compared to recent isolates, with fewer SNPs observed more frequently than expected. Root-to-tip analysis demonstrated a weak linear correlation.</div></div><div><h3>Conclusions</h3><div><em>C. difficile</em> molecular clock estimations may be more complex than previously considered, with periods of spore quiescence potentially complicating analyses.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"93 ","pages":"Article 102953"},"PeriodicalIF":2.5,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence of toxigenic Clostridium botulinum in food products sold in Indian retail markets 在印度零售市场销售的食品中产毒梭菌肉毒杆菌的流行。

IF 2.5 3区生物学

Anaerobe Pub Date : 2025-03-19 DOI: 10.1016/j.anaerobe.2025.102954

Athira Vidyadharan, Arun Jyothi Puthiya Veettil, Athira Pulickal Santhosh, Kuttanapilly Velayudhan Lalitha, Toms Cheriyath Joseph

{"title":"Prevalence of toxigenic Clostridium botulinum in food products sold in Indian retail markets","authors":"Athira Vidyadharan, Arun Jyothi Puthiya Veettil, Athira Pulickal Santhosh, Kuttanapilly Velayudhan Lalitha, Toms Cheriyath Joseph","doi":"10.1016/j.anaerobe.2025.102954","DOIUrl":"10.1016/j.anaerobe.2025.102954","url":null,"abstract":"<div><h3>Introduction</h3><div><em>Clostridium botulinum</em> and botulinum neurotoxins have been recognized as an important food safety hazard. The objective of this study was to screen the prevalence of <em>C. botulinum</em> and botulinum toxin in various food products sold in the retail markets of India.</div></div><div><h3>Material and methods</h3><div>A total of 236 food products collected from Indian retail markets were screened for preformed botulinum toxin using a mouse bioassay. This was followed by enrichment and isolation and further testing of isolates for botulinum toxin production using an additional mouse bioassay. A toxin neutralization test with type specific antitoxins, 16S rRNA gene sequencing and <em>BoNT</em> (botulinum neurotoxin) gene amplification confirmed the presence of <em>C. botulinum</em> and its toxin production potential.</div></div><div><h3>Results and discussion</h3><div>Preformed botulinum toxin was not detected in any of the products. The overall prevalence of <em>C. botulinum</em> in the products was 11 %, but pure cultures of <em>C. botulinum</em> could be isolated only from 10 samples. The isolates were identified as <em>C. botulinum</em> type A, type B and type E based on toxin typing with type specific antitoxins and amplification of the botulinum neurotoxin (<em>BoNT)</em> gene. One of the isolates was designated as <em>C. botulinum</em> subtype A<sub>1</sub>B. This is the first report of isolation of <em>C. botulinum</em> type E and subtype A<sub>1</sub>B in seafood from India. The presence of <em>C. botulinum</em> in the products tested can be a significant public health hazard since the organism can grow when exposed to favourable conditions and can produce neurotoxin in the food.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"93 ","pages":"Article 102954"},"PeriodicalIF":2.5,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular detection of toxigenic Clostridioides difficile in subgingival biofilm of severe periodontitis 重度牙周炎龈下生物膜中产毒艰难梭菌的分子检测。

IF 2.5 3区生物学

Anaerobe Pub Date : 2025-03-15 DOI: 10.1016/j.anaerobe.2025.102955

Isabela Leite de Oliveira Rosa, Eliane de Oliveira Ferreira, Ana Paula Vieira Colombo

{"title":"Molecular detection of toxigenic Clostridioides difficile in subgingival biofilm of severe periodontitis","authors":"Isabela Leite de Oliveira Rosa, Eliane de Oliveira Ferreira, Ana Paula Vieira Colombo","doi":"10.1016/j.anaerobe.2025.102955","DOIUrl":"10.1016/j.anaerobe.2025.102955","url":null,"abstract":"<div><h3>Objectives</h3><div>The oral cavity is the main gateway for the entry of <em>C. difficile</em> spores to the digestive tract. In conditions of poor oral hygiene and periodontal diseases, the dysbiotic oral microbiota may be a reservoir for several human pathogens. Here, we explored the prevalence of <em>C. difficile</em> in the oral microbiota of patients with severe periodontitis by the molecular detection of species specific genes.</div></div><div><h3>Methods</h3><div>Subgingival biofilm, saliva and/or feces from 659 patients with gingivitis, periodontitis and no periodontal diseases were screened for the <em>tpi</em> and toxin A/B genes specific for <em>C. difficile</em> by multiplex PCR. Differences among groups were sought by the Chi-square test.</div></div><div><h3>Results</h3><div>The overall frequency of <em>C. difficile tpi</em> gene was 29 %, with a high detection of <em>tcd</em>B gene (44.8 %). Patients with periodontitis showed a greater prevalence of this gene in the biofilm than individuals with gingivitis and periodontal health (p = 0.001), particularly at more severe stages of disease (p < 0.05). No toxin genes were detected in feces or biofilm from healthy patients, whereas >70 % of the biofilm from patients with periodontal diseases were positive for these genes (p < 0.001). Detection of <em>C. difficile tpi</em> gene in oral/fecal samples correlated with periodontal inflammation (p < 0.05). A modest intra-individual agreement between <em>tpi</em> gene detection in feces and saliva was found within periodontitis patients (Kappa = 0.314; p = 0.003).</div></div><div><h3>Conclusion</h3><div>The high frequency of the <em>C. difficile</em> specific genes <em>tpi</em> and <em>tcd</em>B in the dysbiotic subgingival biofilm of advanced periodontitis could support the presence of the bacterium in this niche.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"93 ","pages":"Article 102955"},"PeriodicalIF":2.5,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143647230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a polymicrobial host-bacterial interface biofilm model for bacterial vaginosis 细菌性阴道病多微生物宿主-细菌界面生物膜模型的建立。

IF 2.5 3区生物学

Anaerobe Pub Date : 2025-03-08 DOI: 10.1016/j.anaerobe.2025.102952

William Johnston, Ryan Kean

引用次数: 0

A retrospective study comparing the effectiveness of carbapenems and tazobactam/piperacillin as an empirical treatment for patients infected with Bacteroides fragilis 碳青霉烯类药物与他唑巴坦/哌拉西林治疗脆弱拟杆菌感染的回顾性研究

IF 2.5 3区生物学

Anaerobe Pub Date : 2025-03-01 DOI: 10.1016/j.anaerobe.2025.102950

Hideo Kato , Mao Hagihara , Nobuhiro Asai , Hiroshige Mikamo , Takuya Iwamoto

{"title":"A retrospective study comparing the effectiveness of carbapenems and tazobactam/piperacillin as an empirical treatment for patients infected with Bacteroides fragilis","authors":"Hideo Kato , Mao Hagihara , Nobuhiro Asai , Hiroshige Mikamo , Takuya Iwamoto","doi":"10.1016/j.anaerobe.2025.102950","DOIUrl":"10.1016/j.anaerobe.2025.102950","url":null,"abstract":"<div><h3>Background</h3><div><em>Bacteroides fragilis</em> is one of the most frequently isolated pathogenic anaerobic bacteria. Infections caused by <em>B. fragilis</em> are commonly treated with carbapenems or tazobactam/piperacillin. However, there is no evidence for the optimal antibiotic choice against infections caused by <em>B. fragilis</em> as an empirical therapy owing to its varied antibiotic resistance mechanisms. Hence, we compared the effectiveness of carbapenems and tazobactam/piperacillin as an antibiotic therapy in patients infected with <em>B. fragilis</em>.</div></div><div><h3>Methods</h3><div>We investigated mortality, clinical and antimicrobial efficacy, and the percentage of patients who switched to broad-spectrum antibiotics, such as carbapenems or tazobactam/piperacillin, due to the aggravated symptoms of infection in patients receiving carbapenems or tazobactam/piperacillin from 2019 to 2024.</div></div><div><h3>Results</h3><div>A total of 60 patients were included in the study; 24 patients received carbapenems and 36 received tazobactam-piperacillin as an empirical treatment against <em>B. fragilis</em> infections. None of the patients in either group died. Compared to carbapenem treatment, tazobactam/piperacillin treatment significantly improved inflammatory markers, including body temperature and C-reactive protein (45.8 % vs. 72.2 %, <em>p</em> = 0.039 and 37.5 % vs. 63.4 %, <em>p</em> = 0.045, respectively; carbapenem vs. tazobactam/piperacillin). The percentage of patients switching from carbapenems to tazobactam/piperacillin or vice versa was significantly lower in the tazobactam/piperacillin group than in the carbapenem group (41.7 % vs. 11.1 %, <em>p</em> < 0.001).</div></div><div><h3>Conclusions</h3><div>Our findings suggest that tazobactam/piperacillin can be used as the first-line empirical treatment for patients infected with <em>B. fragilis</em>.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"92 ","pages":"Article 102950"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143539745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polymorphonuclear neutrophil depletion in ileal tissues reduces the immunopathology induced by Clostridioides difficile toxins 回肠组织中多形核中性粒细胞耗竭可减少艰难梭菌毒素引起的免疫病理。

IF 2.5 3区生物学

Anaerobe Pub Date : 2025-02-27 DOI: 10.1016/j.anaerobe.2025.102947

Montoya-Torres Brayan , Alfaro-Alarcón Alejandro , Quesada-Gómez Carlos , Chaves-Olarte Esteban , Barquero-Calvo Elías

{"title":"Polymorphonuclear neutrophil depletion in ileal tissues reduces the immunopathology induced by Clostridioides difficile toxins","authors":"Montoya-Torres Brayan , Alfaro-Alarcón Alejandro , Quesada-Gómez Carlos , Chaves-Olarte Esteban , Barquero-Calvo Elías","doi":"10.1016/j.anaerobe.2025.102947","DOIUrl":"10.1016/j.anaerobe.2025.102947","url":null,"abstract":"<div><h3>Introduction</h3><div><em>Clostridioides difficile</em>, a leading cause of healthcare-associated infections, causes significant morbidity and mortality. Its pathogenesis centers on TcdA and TcdB toxins, which disrupt intestinal integrity, trigger inflammation, and promote extensive neutrophil infiltration.</div></div><div><h3>Objective</h3><div>The main objective of this study was to evaluate the role of PMNs in CDI using neutrophil depletion in a murine-ileal-ligated loop.</div></div><div><h3>Methods</h3><div>Mice were treated with <em>C. difficile</em> toxins TcdA, TcdB, and TcdBv, with PMN depletion achieved via intraperitoneal injections of Ly6G/Ly6C antibody. Histopathological analysis, cytokine quantification, and MPO activity assays were performed to assess the inflammatory and tissue damage responses.</div></div><div><h3>Results</h3><div>PMN depletion significantly reduced histopathological damage and proinflammatory responses. TcdA induced the highest inflammation and epithelial damage, while TcdB showed lower activity, except for MPO. TcdBv<sub>NAP1</sub>'s activity was comparable to that of TcdB<sub>NAP1</sub> but less than TcdA. The findings indicate that TcdA's enterotoxin effects are more damaging than TcdBs from different strains and confirm the critical role of PMNs in CDI pathogenesis.</div></div><div><h3>Conclusion</h3><div>Our results show that PMN depletion reduced inflammatory responses and tissue damage, highlighting potential therapeutic strategies targeting PMN regulation. Further research on PMN extracellular traps (NETs) and their role in CDI is necessary to develop comprehensive treatments. Future studies should focus on combined <em>in vivo</em> and <em>in vitro</em> approaches to fully understand the pathological mechanisms and identify effective biomarkers for CDI therapy.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"92 ","pages":"Article 102947"},"PeriodicalIF":2.5,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143536405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Growth of hydrogenotrophic methanogen Methanoculleus bourgensis MAB1 in the presence of dunite 养氢甲烷菌 Methanoculleus bourgensis MAB1 在白云石存在下的生长。

IF 2.5 3区生物学

Anaerobe Pub Date : 2025-02-26 DOI: 10.1016/j.anaerobe.2025.102945

A. Neubeck , N. Callac , S. Isaksson , A. Schnürer

引用次数: 0