Anaerobe最新文献

{"title":"Clostridioides difficile is a bacterial priority pathogen","authors":"Wiep Klaas Smits , Kevin W. Garey , Thomas V. Riley , Stuart Johnson","doi":"10.1016/j.anaerobe.2025.102965","DOIUrl":"10.1016/j.anaerobe.2025.102965","url":null,"abstract":"","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"93 ","pages":"Article 102965"},"PeriodicalIF":2.5,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CDBN-YGXZ shows highly antibacterial activity against Clostridioides difficile as a potential candidate drug","authors":"Xiaoxia Zhang ,&nbsp;Min Quan ,&nbsp;Xin Lu ,&nbsp;Linwan Zhang ,&nbsp;Bing Guang ,&nbsp;Zhiyong Zong ,&nbsp;Xiaohui Wang","doi":"10.1016/j.anaerobe.2025.102964","DOIUrl":"10.1016/j.anaerobe.2025.102964","url":null,"abstract":"<div><div>CDBN-YGXZ shows a low minimal inhibitory concentration range from 0.015 to 0.125 mg/L against clinical <em>Clostridioides difficile</em> strains with diverse genotypes, hardly induces bacterial resistance, and intensively inhibits 86.2 % of <em>C. difficile</em> adhesion to intestinal epithelial cells Caco-2. CDBN-YGXZ shows highly antibacterial activity and is potentially a candidate as a therapeutic drug.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"93 ","pages":"Article 102964"},"PeriodicalIF":2.5,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143881297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An investigation into breast infections caused by Actinomyces species","authors":"Karla Berry ,&nbsp;Aleksandra Marek ,&nbsp;Mairi MacLeod ,&nbsp;Abhijit M. Bal","doi":"10.1016/j.anaerobe.2025.102966","DOIUrl":"10.1016/j.anaerobe.2025.102966","url":null,"abstract":"<div><div>We investigated the management of <em>Actinomyces</em> breast infection. Eight different <em>Actinomyces</em> species were isolated from 26 patients during the study period (December 2018–November 2021). All tested isolates were susceptible (at standard or high dose) to penicillin and 75 % to clindamycin. Twenty-three patients had a history of smoking. Three had coexisting breast cancer. Prescribers need to be educated about the lack of activity of metronidazole on <em>Actinomyces</em>.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"93 ","pages":"Article 102966"},"PeriodicalIF":2.5,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143882539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacteremia due to Hungatella effluvii in a patient with end-stage renal failure requiring hemodialysis: a case report and literature review of human infections caused by Hungatella spp.","authors":"Yukitaka Ito ,&nbsp;Yuri Tanaka ,&nbsp;Naomi Kaneko ,&nbsp;Mieko Yoshida ,&nbsp;Nobuhiko Joki","doi":"10.1016/j.anaerobe.2025.102962","DOIUrl":"10.1016/j.anaerobe.2025.102962","url":null,"abstract":"<div><div><em>Hungatella effluvii</em> infections in humans are rare. We report a case of <em>H. effluvii</em> bacteremia in a Japanese male with chronic kidney disease identified by 16S rRNA gene sequencing after initial misidentification as <em>Clostridium</em> species. Despite treatment, the patient died of sepsis related to a nephrostomy catheter infection. A literature review suggests that <em>Hungatella</em> spp. infections may be associated with severe diseases, such as sepsis and necrotizing fasciitis. This case highlights the difficulty in microbial identification, as biochemical methods were insufficient, requiring molecular biological methods. Antimicrobial susceptibility testing (AST) revealed resistance to benzylpenicillin and moxifloxacin, consistent with prior reports. This is the third documented case of <em>H. effluvii</em> infection, emphasizing the need for accurate pathogen identification and AST to guide optimal treatment in rare infections.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"93 ","pages":"Article 102962"},"PeriodicalIF":2.5,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143887024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro antimicrobial efficacy of Cannabis sativa L. (hemp) crude oil extract on Fusobacterium necrophorum of bovine origin","authors":"Jourdan E. Lakes ,&nbsp;Alexander W. Altman ,&nbsp;Mark A. Berhow ,&nbsp;Isabelle A. Kagan ,&nbsp;T.G. Nagaraja ,&nbsp;David L. Harmon ,&nbsp;Michael D. Flythe","doi":"10.1016/j.anaerobe.2025.102960","DOIUrl":"10.1016/j.anaerobe.2025.102960","url":null,"abstract":"<div><div><em>Fusobacterium necrophorum</em> commonly causes liver abscesses in grain-finished beef, which is mitigated using antibiotics in feed. However, microbial resistance development necessitates exploration of antibiotic alternatives. Here we report the antimicrobial efficacy of <em>Cannabis sativa</em> L. (hemp) crude oil extract and its constituents on <em>Fusobacterium</em> species.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"93 ","pages":"Article 102960"},"PeriodicalIF":2.5,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clostridioides (Clostridium) difficile in hospitalised children in Cambodia","authors":"Lengsea Eng ,&nbsp;Kefyalew Addis Alene ,&nbsp;Deirdre A. Collins ,&nbsp;Su-Chen Lim ,&nbsp;Viso Srey ,&nbsp;Choeung Chea ,&nbsp;Sotera Yohn ,&nbsp;Setha Leng ,&nbsp;Archie C.A. Clements ,&nbsp;Thomas V. Riley","doi":"10.1016/j.anaerobe.2025.102959","DOIUrl":"10.1016/j.anaerobe.2025.102959","url":null,"abstract":"<div><h3>Background</h3><div>Children may play an important role in disseminating <em>Clostridioides</em> (<em>Clostridium</em>) <em>difficile</em> within hospital and community settings. In many parts of the world, there has been a recent increase in interest in <em>C. difficile</em> infection (CDI) in paediatric populations.</div></div><div><h3>Objectives</h3><div>This study aimed to investigate the prevalence of, risk factors for, and molecular types of <em>C. difficile</em> in hospitalised children in Cambodia.</div></div><div><h3>Methods</h3><div>Stool samples were collected from children at the National Paediatric Hospital in Phnom Penh, Cambodia, between June 2022 and March 2023, for <em>C. difficile</em> culture. Toxin gene PCR and PCR ribotyping were performed on all isolates.</div></div><div><h3>Results</h3><div>Of 122 hospitalised children recruited, <em>C. difficile</em> was identified in 47 (38.5 %). Toxigenic strains accounted for 25.5 % (12/47), with ribotype (RT) 012 the most predominant (7/12), followed by RTs 014/020 (3/12) and 017 (1/12). Non-toxigenic strains were dominated by RTs QX011, QX675 and 009/QX107. Novel strains represented 70 % (33/47) of isolates. Significant risk factors included antimicrobial use in the week before detection (OR = 5.15; 95 %CI: 1.125–21.21) and current use of other medications (OR = 13.02, 95 %CI: 1.32–128.67). Diarrhoea and abdominal pain were negatively associated with the presence of <em>C. difficile.</em></div></div><div><h3>Conclusions</h3><div>A high prevalence of <em>C. difficile</em> was found in hospitalised children, with a high proportion of non-toxigenic and novel strains. Larger studies are required with whole genome sequencing necessary for characterising novel strains and advancing molecular epidemiological research in Asia.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"93 ","pages":"Article 102959"},"PeriodicalIF":2.5,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143876714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Lachnoanaerobaculum sanguinis sp. nov., isolated from a blood culture of an acute myeloid leukemia patient with chemotherapy-related bacteremia","authors":"Yumi Suenari ,&nbsp;Toshiyuki Matsubara ,&nbsp;Yuka Hiroshima ,&nbsp;Muhammad Reza Pahlevi ,&nbsp;Hiroka Shimada ,&nbsp;Yuto Ochi ,&nbsp;Naoto Okada ,&nbsp;Masami Sato ,&nbsp;Hideki Fujii ,&nbsp;Tomoko Sumitomo ,&nbsp;Hidehiro Kishimoto ,&nbsp;Akikazu Murakami","doi":"10.1016/j.anaerobe.2025.102961","DOIUrl":"10.1016/j.anaerobe.2025.102961","url":null,"abstract":"<div><h3>Objective</h3><div>This study was to characterize and determine the classification of the novel strain TM49^T, isolated from a blood culture of a patient who developed bacteremia while receiving high-dose cytarabine chemotherapy for acute myeloid leukemia.</div></div><div><h3>Methods</h3><div>The novel strain TM49^T was characterized at the species level through a polyphasic taxonomic analysis, incorporating morphological, physiological, biochemical, phylogenetic, and genome sequence analyses.</div></div><div><h3>Results</h3><div>16S rRNA gene sequence analysis revealed that the strain belonged to the genus <em>Lachnoanaerobaculum</em>. The 16S rRNA gene sequence of strain TM49^T was observed to be most similar to that of \"<em>Lachnoanaerobaculum gingivalis</em>\" strain ChDC B114^T (99.2 %). Strain TM49^T formed circular, glossy, slightly milky colonies with an undulate margin on BD™ Columbia Agar containing 5 % sheep blood after 48 h at 37 °C, exhibiting less spreading compared to species of the genus <em>Lachnoanaerobaculum</em>. The major fatty acids of strain TM49^T were C_16:0, C_14:0, C_18:1 ω9c DMA, and C_18:1 ω7c DMA. The draft genome sequence of strain TM49^T was 2,821,554 bp in length, with a G + C content of 36.4. Digital DNA-DNA hybridization values between strain TM49^T and “<em>L. gingivalis</em>” ChDC B114^T, <em>L. umeaense</em> CD3: 22^T, <em>L. orale</em> N1^T, and <em>L. saburreum</em> CCUG 28089^T were 52.2 %, 29.7 %, 26.2 %, and 24.5 %, respectively. The Orthologous Average Nucleotide Identity values between strain TM49^T and “<em>L. gingivalis</em>” ChDC B114^T, <em>L. umeaense</em> CD3: 22^T, <em>L. orale</em> N1^T, and <em>L. saburreum</em> CCUG 28089^T were 93.1 %, 84.9 %, 83.2 %, and 81.5 %, respectively.</div></div><div><h3>Conclusion</h3><div>Strain TM49^T (JCM 36186^T = DSM 116945^T) was found to be distinct from previously described species of the genus <em>Lachnoanaerobaculum</em>. Therefore, it is proposed as a new species, <em>Lachnoanaerobaculum sanguinis</em> sp. Nov., with strain TM49^T as the type strain (= JCM 36186^T = DSM 116945^T).</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"93 ","pages":"Article 102961"},"PeriodicalIF":2.5,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel competitive annealing-mediated isothermal amplification (CAMP)-based detection of epsilon toxin gene in Clostridium perfringens","authors":"A. Arun Prince Milton ,&nbsp;Torik Basar ,&nbsp;K. Srinivas ,&nbsp;Aleimo G. Momin ,&nbsp;Sabia Khan ,&nbsp;G. Bhuvana Priya ,&nbsp;Samir Das ,&nbsp;S. Ghatak ,&nbsp;K. Puro","doi":"10.1016/j.anaerobe.2025.102958","DOIUrl":"10.1016/j.anaerobe.2025.102958","url":null,"abstract":"<div><div>In this study, we developed a novel competitive annealing-mediated isothermal amplification (CAMP)-based assay for the detection of the <em>etx</em> gene, which encodes the epsilon toxin of <em>Clostridium perfringens</em>. The assay, performed at 60 °C for 60 min, demonstrated 100 % specificity and was 100 times more sensitive than endpoint PCR.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"93 ","pages":"Article 102958"},"PeriodicalIF":2.5,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cultivation of anaerobic bacteria: Foundations and principles","authors":"Paul A. Lawson,&nbsp;Ralph S. Tanner","doi":"10.1016/j.anaerobe.2025.102951","DOIUrl":"10.1016/j.anaerobe.2025.102951","url":null,"abstract":"<div><div>A brief history of techniques in anaerobic microbiology are presented leading up to the incorporation of several improvements we have used over the years to improve our culture of anaerobic microorganisms of environmental, industrial and clinical importance. Two overriding aspects from our combined 90 years of experience here are: the better one's control of anaerobic conditions and gas phases, the better results are obtained; techniques can and should be targeted for individual microorganisms and accompanying experiments. Continued improvements in anaerobic microbiology are expected and encouraged for the future.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"93 ","pages":"Article 102951"},"PeriodicalIF":2.5,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143724611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial resistance profiles of Clostridium species isolated from post-traumatic infections in a Costa Rican hospital","authors":"Mariela Alvarado-Rodríguez ,&nbsp;Carlos Quesada-Gómez","doi":"10.1016/j.anaerobe.2025.102957","DOIUrl":"10.1016/j.anaerobe.2025.102957","url":null,"abstract":"<div><div>This study analyzed antimicrobial resistance in 119 <em>Clostridium</em> species isolates collected at a Costa Rican trauma hospital (2018–2022). Isolates were identified using MALDI-TOF, and antimicrobial susceptibility was assessed by E-test. <em>C. perfringens, C. tertium,</em> and <em>Paeniclostridium sordellii</em> were most prevalent species. All isolates were susceptible to amoxicillin-clavulanic acid, linezolid, and metronidazole; however, resistance rates of 20 % to vancomycin and 25 % to clindamycin were observed. <em>C. sphenoides</em> showed the highest minimum inhibitory concentration values. Based on these findings, empirical treatment strategies at the hospital now prioritize amoxicillin-clavulanic acid, linezolid, and metronidazole, while reducing reliance on clindamycin due to observed resistance patterns.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"93 ","pages":"Article 102957"},"PeriodicalIF":2.5,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}