基于竞争退火介导的等温扩增（CAMP）检测产气荚膜梭菌epsilon毒素基因的新方法。

IF 2.5 3区生物学 Q3 MICROBIOLOGY

Anaerobe Pub Date : 2025-03-26 DOI:10.1016/j.anaerobe.2025.102958

A. Arun Prince Milton , Torik Basar , K. Srinivas , Aleimo G. Momin , Sabia Khan , G. Bhuvana Priya , Samir Das , S. Ghatak , K. Puro

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引用次数: 0

摘要

在这项研究中，我们开发了一种新的基于竞争退火介导的等温扩增（CAMP）的检测方法，用于检测产气荚膜梭菌（Clostridium perfringens）中编码epsilon毒素的etx基因。实验在60°C下进行60分钟，显示出100%的特异性，比终点PCR灵敏100倍。

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Novel competitive annealing-mediated isothermal amplification (CAMP)-based detection of epsilon toxin gene in Clostridium perfringens

In this study, we developed a novel competitive annealing-mediated isothermal amplification (CAMP)-based assay for the detection of the etx gene, which encodes the epsilon toxin of Clostridium perfringens. The assay, performed at 60 °C for 60 min, demonstrated 100 % specificity and was 100 times more sensitive than endpoint PCR.

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来源期刊

Anaerobe 生物-微生物学

CiteScore

5.20

自引率

8.70%

发文量

137

审稿时长

76 days

期刊介绍： Anaerobe is essential reading for those who wish to remain at the forefront of discoveries relating to life processes of strictly anaerobes. The journal is multi-disciplinary, and provides a unique forum for those investigating anaerobic organisms that cause infections in humans and animals, as well as anaerobes that play roles in microbiomes or environmental processes. Anaerobe publishes reviews, mini reviews, original research articles, notes and case reports. Relevant topics fall into the broad categories of anaerobes in human and animal diseases, anaerobes in the microbiome, anaerobes in the environment, diagnosis of anaerobes in clinical microbiology laboratories, molecular biology, genetics, pathogenesis, toxins and antibiotic susceptibility of anaerobic bacteria.