Anaerobe最新文献

{"title":"Immunogenicity of a recombinant chimera composed of CROP domain segments from the hemorrhagic and lethal toxins of Paeniclostridium sordellii","authors":"Rafael Rodrigues Rodrigues ,&nbsp;Neida Conrad ,&nbsp;Marcos Roberto Alves Ferreira ,&nbsp;Clóvis Moreira Júnior ,&nbsp;Mariliana Luiza Ferreira Alves ,&nbsp;Pamela Aristimunho Sedrez ,&nbsp;Vitória Müller ,&nbsp;Alessandra Neis ,&nbsp;Miguel Andrade Bilhalva ,&nbsp;Cleideanny Cancela Galvão ,&nbsp;Fábio Pereira Leivas Leite ,&nbsp;Fabricio Rochedo Conceição","doi":"10.1016/j.anaerobe.2025.102938","DOIUrl":"10.1016/j.anaerobe.2025.102938","url":null,"abstract":"<div><div><em>Paeniclostridium sordellii</em> is responsible for severe infections in horses, cattle, and sheep, however, conventional vaccines exhibit limitations in production and immunogenicity. This study assessed the immunogenicity of a recombinant bacterin composed of a chimera (rQTcsHL) that combines segments from the lethal (TcsL) and hemorrhagic (TcsH) toxins in mice and sheep. Both immunized animal groups exhibited elevated levels of IgG, with the mice demonstrating moderate protection (&lt;50 %) against lethal challenges, comparable to that of the conventional vaccine. Further molecular optimization is essential to enhance its efficacy.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"91 ","pages":"Article 102938"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142963283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hypopituitarism secondary to internal carotid artery pseudoaneurysm following Lemierre's syndrome caused by Fusobacterium nucleatum","authors":"Christiane Gruetzmacher ,&nbsp;Marilena Nakaguma ,&nbsp;Raquel Soares Jallad ,&nbsp;Flora Ladeira Craveiro ,&nbsp;Ana Paula da Silva Tenorio ,&nbsp;Mario Padula ,&nbsp;Rafael Loch Batista","doi":"10.1016/j.anaerobe.2025.102942","DOIUrl":"10.1016/j.anaerobe.2025.102942","url":null,"abstract":"<div><div>Lemierre's syndrome (LS) is a rare complication of oropharyngeal infections, often causing sepsis, internal jugular vein thrombosis, and metastatic infections. We present a unique case of a 48-year-old male with LS complicated by an internal carotid artery pseudoaneurysm compressing the pituitary gland, resulting in panhypopituitarism. Diagnosis was confirmed via imaging and detection of <em>Fusobacterium nucleatum</em>. Treatment included antibiotics, anticoagulation, and hormone replacement. Although the patient recovered from pituitary deficiencies, growth hormone deficiency persisted. This case emphasizes the importance of recognizing endocrine dysfunctions in LS and highlights the need for multidisciplinary management to address vascular and neuroendocrine complications.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"92 ","pages":"Article 102942"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143122029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A rare isolation of Parvimonas micra from cerebellar abscess in a patient with complex cyanotic heart disease","authors":"Praveen Ravichandran ,&nbsp;Ramit Kundu ,&nbsp;Rakhi Biswas ,&nbsp;Sathiaprabhu Anbazhagan","doi":"10.1016/j.anaerobe.2025.102943","DOIUrl":"10.1016/j.anaerobe.2025.102943","url":null,"abstract":"<div><div><em>Parvimonas micra</em> is a gram-positive coccus which is an obligate anaerobe. It is a commensal in the oropharyngeal cavity and the gastrointestinal tract. The genus <em>Parvimonas</em> has only a single species, <em>P. micra</em>. Although there are several reports of infections due to <em>P. micra</em> involving various body sites, cases of brain abscess due to this organism have been under-reported in the literature. Here we present a rare case of cerebellar abscess caused by <em>P. micra</em> in a nine-year-old boy with complex cyanotic heart disease<strong>.</strong></div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"93 ","pages":"Article 102943"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulatory networks: Linking toxin production and sporulation in Clostridioides difficile","authors":"Md Kamrul Hasan,&nbsp;Oluchi Alaribe,&nbsp;Revathi Govind","doi":"10.1016/j.anaerobe.2024.102920","DOIUrl":"10.1016/j.anaerobe.2024.102920","url":null,"abstract":"<div><div><em>Clostridioides difficile</em> has been recognized as an important nosocomial pathogen that causes diarrheal disease as a consequence of antibiotic exposure and costs the healthcare system billions of dollars every year. <em>C. difficile</em> enters the host gut as dormant spores, germinates into vegetative cells, colonizes the gut, and produces toxins TcdA and/or TcdB, leading to diarrhea and inflammation. Spores are the primary transmission vehicle, while the toxins A and B directly contribute to the disease. Thus, toxin production and sporulation are the key traits that determine the success of <em>C. difficile</em> as a pathogen. Both toxins and spores are produced during the late stationary phase in response to various stimuli. This review provides a comprehensive analysis of the current knowledge on the molecular mechanisms, highlighting the regulatory pathways that interconnect toxin gene expression and sporulation in <em>C. difficile</em>. The roles of carbohydrates, amino acids and other nutrients and signals, in modulating these virulence traits through global regulatory networks are discussed. Understanding the links within the gene regulatory network is crucial for developing effective therapeutic strategies against <em>C. difficile</em> infections, potentially leading to targeted interventions that disrupt the co-regulation of toxin production and sporulation.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"91 ","pages":"Article 102920"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New insights into the factors influencing methanogenic pathways in anaerobic digesters","authors":"Helena Rodrigues Oliveira ,&nbsp;Thuane Mendes Anacleto ,&nbsp;Fernanda Abreu ,&nbsp;Alex Enrich-Prast","doi":"10.1016/j.anaerobe.2024.102925","DOIUrl":"10.1016/j.anaerobe.2024.102925","url":null,"abstract":"<div><h3>Introduction</h3><div>Anaerobic digestion integrates waste treatment, energy generation, and nutrient recycling, producing methane mainly through acetoclastic (AM) and hydrogenotrophic methanogenesis (HM). Methanogenic pathway management can improve biogas productivity and quality. The balance between pathways is influenced by environmental and physicochemical conditions, with conflicting results on the effect of different factors often reported. This systematic review aims to clarify the influence of various parameters on methanogenic pathways in anaerobic digesters.</div></div><div><h3>Methods</h3><div>Literature search was conducted in the Web of Science and Scopus databases. The effects of different parameters on the predominant methanogenic pathway were evaluated using Kruskal-Wallis tests and Spearman's rank correlation.</div></div><div><h3>Results</h3><div>Thermophilic temperatures and high free ammonia nitrogen concentrations (&gt;300 mg L⁻¹) increase HM, with a strong combined effect of these variables. Conversely, under moderate temperature and ammonia concentrations, the primary feedstock influences the methanogenic pathway, with algae biomass, pig manure, and food industry wastewater showing the lowest contribution of hydrogenotrophic methanogens. pH effect varied with temperature, with acidic and alkaline pH favoring HM in mesophilic and thermophilic digesters, respectively. Furthermore, higher levels of volatile fatty acids (&gt;2000 mg L⁻¹), carbohydrates (&gt;10 g/L) and lipids (&gt;10 g/L) also appeared to favor HM over AM, while most metals – especially Cr, Se and W – promoted AM.</div></div><div><h3>Conclusion</h3><div>This study emphasizes the role of various factors in methanogenic pathway selection, highlighting the impact of previously overlooked parameters, such as inorganic elements and organic matter composition. These insights are essential for understanding the methanogenic pathway balance and optimizing biogas processes.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"91 ","pages":"Article 102925"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A rapid genome-proteome approach to identify rate-limiting steps in the butyrate production pathway in probiotic Clostridium butyricum, CBM588","authors":"Liam Wood ,&nbsp;Bunmi B. Omorotionmwan ,&nbsp;Adam M. Blanchard ,&nbsp;Adam Dowle ,&nbsp;David Tooth ,&nbsp;Tom S. Bailey ,&nbsp;Ruth Griffin","doi":"10.1016/j.anaerobe.2025.102940","DOIUrl":"10.1016/j.anaerobe.2025.102940","url":null,"abstract":"<div><h3>Objectives</h3><div><em>Clostridium butyricum</em> ferments non-digested dietary fibre in the colon to produce butyric acid. Butyrate is a four-carbon, short-chain fatty acid (SCFA) which has multiple health benefits. Many microbial products of pharmaceutical or industrial interest, such as butyrate, are produced in low quantities due to rate-limiting steps in their metabolic pathway, including low abundance or low activity of one or more enzymes in the pathway. By identifying the former, appropriate enzymes can be over-expressed to increase product yields, however, methods to determine these enzymes are laborious. To improve butyrate production in <em>C. butyricum</em> probiotic strain, CBM588, a novel rapid genome-proteome approach was deployed.</div></div><div><h3>Methods</h3><div>First, whole genome sequencing was performed and the 8 genes involved in butyrate production identified on the chromosome. Second, the relative abundance of these enzymes was investigated by liquid chromatography-mass spectrometry (LC-MS) analysis of total cytosolic proteins from early stationary phase cultures.</div></div><div><h3>Results</h3><div>Phosphotransbutyrylase (Ptb), butyrate kinase (Buk) and crotonase (Crt) were found to be the least abundant. Over-expression episomally of the corresponding genes individually or of the <em>ptb-buk</em> bicistron led to significant increases in butyrate titre per density of culture from 10 to 24 h, compared to the wild type.</div></div><div><h3>Conclusions</h3><div>Our findings pave the way for over-expressing these genes chromosomally to generate a recombinant probiotic with improved butyrate production and potentially enhanced gut health properties.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"92 ","pages":"Article 102940"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitin promotes equol production via n-acetylglucosamine in human fecal cultures","authors":"Miyuki Kodera ,&nbsp;Kohei Nakamura ,&nbsp;S. Yokoyama","doi":"10.1016/j.anaerobe.2024.102923","DOIUrl":"10.1016/j.anaerobe.2024.102923","url":null,"abstract":"<div><h3>Objectives</h3><div>Equol is a metabolite of isoflavone. Here, we investigated the effect of dietary fiber on equol-producing microbiota.</div></div><div><h3>Methods</h3><div>Human feces was anaerobically cultured in Gifu Anaerobic Medium (GAM) containing 1 mg/L daidzein supplemented with 1 % (w/v) dietary fiber (pectin, chitin, or resistant starch [RS]). The equol conversion rate, number of equol-producing bacteria, pH, short-chain fatty acids (SCFAs), and microbial composition were measured in fecal cultures.</div></div><div><h3>Results</h3><div>Equol conversion significantly increased by 2.7-fold in the chitin group compared with the control group. Conversely, the conversion rate decreased to one-tenth in the pectin group and one-third in the RS group. The number of equol-producing bacteria also significantly increased in the chitin group compared with the control group. Whereas pH values and acetate/propionate concentrations decreased in the pectin and RS groups compared with the control group, no significant differences were observed in the chitin group. Microbiota analysis indicated that the relative abundances of <em>Bifidobacterium</em> and <em>Lactobacillus</em> increased, whereas those of <em>Dorea</em>, <em>Lachnoclostridium</em>, and <em>Eggerthella</em> decreased in the pectin and RS groups. In contrast, these abundances remained stable in the chitin group. The relative abundances of <em>Adlercreutzia</em> and <em>Slackia</em> remained unchanged across all groups. <em>In vitro</em> cultures of <em>Eggerthella</em> sp. strain YY7918 and <em>Adlercreutzia equolifaicens</em> JCM14793 demonstrated that equol conversion from daidzein was stimulated by the addition of 0.5–1% (w/v) <span><em>n</em></span>-acetylglucosamine, a breakdown product of chitin, but not chitin itself.</div></div><div><h3>Conclusions</h3><div>Our findings suggest that equol production is stimulated by <span><em>n</em></span>-acetylglucosamine, derived from decomposed chitin, in certain equol-producing bacteria without affecting their growth.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"91 ","pages":"Article 102923"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142738151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A rubrerythrin locus of Clostridioides difficile encodes enzymes that efficiently detoxify reactive oxygen species","authors":"Robert Knop,&nbsp;Simon Keweloh,&nbsp;Johanna Pukall,&nbsp;Silvia Dittmann,&nbsp;Daniela Zühlke,&nbsp;Susanne Sievers","doi":"10.1016/j.anaerobe.2025.102941","DOIUrl":"10.1016/j.anaerobe.2025.102941","url":null,"abstract":"<div><h3>Objectives</h3><div>The microaerophilic conditions in the large intestine and reactive oxygen species (ROS) produced by the immune system represent a challenge for the strictly anaerobic pathogen <em>Clostridioides difficile</em>, which protects itself by a variety of oxidative stress proteins. Four of these are encoded in an operon that has been implicated in the detoxification of H₂O₂ and O₂^●-. In this study, proteins of this operon, i. e. a rubrerythrin (Rbr), a superoxide reductase (Sor) and a putative glutamate dehydrogenase (CD630_08280) were investigated for their ROS detoxifying activity <em>in vitro</em>.</div></div><div><h3>Methods</h3><div>Recombinant proteins were overexpressed in <em>C. difficile</em> and purified anaerobically by affinity chromatography. The H₂O₂-reductase activity was determined by measuring the NADH consumption after peroxide addition. Superoxide detoxification potential of Sor was detected colorimetrically using a xanthine/xanthine oxidase system with cytochrome <em>c</em> as analytical probe.</div></div><div><h3>Results</h3><div>Proposed roles of the investigated proteins in the detoxification pathways of ROS could partially be demonstrated. Specifically, Rbr and glutamate dehydrogenase synergistically detoxify H₂O₂, although with a very low turnover. Furthermore, Sor was shown to scavenge O₂^●- by superoxide dismutase activity and its activity was compared to superoxide dismutase of <em>Escherichia coli</em>.</div></div><div><h3>Conclusions</h3><div>The investigated gene locus codes for an oxidative stress operon whose members have the potential to neutralize O₂^●- and H₂O₂ to water and thus complements the arsenal of ROS detoxifying mechanisms that are already known in <em>C. difficile</em>. However, full activity with adequate physiological electron transfer partners still needs to be demonstrated.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"92 ","pages":"Article 102941"},"PeriodicalIF":2.5,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fulminant Clostridioides (Costridium) difficile infection caused by a rare strain of PCR-ribotype 153 in Japan: A case report.","authors":"Daichi Yomogida, Suguru Hasegawa, Shiori Mizuta, Shinjiro Horikawa, Yoshinao Koshida, Koichiro Matsuda, Masahiko Nakamura, Hiroyasu Kaya, Akio Uchiyama, Mitsutoshi Senoh","doi":"10.1016/j.anaerobe.2024.102936","DOIUrl":"https://doi.org/10.1016/j.anaerobe.2024.102936","url":null,"abstract":"<p><p>Clostridioides (Clostridium) difficile (C. difficile) infection (CDI), often severe when producing toxin A, toxin B, and CDT, can cause life-threatening fulminant infections, especially in vulnerable patients. This case report discusses a 39-year-old woman with no medical history who developed severe CDI after antibiotic treatment, leading to fatal hypovolemic shock. A rare C. difficile PCR-ribotype 153 strain which is positive for toxin A, toxin B, and CDT was identified. This case emphasizes the need for early CDI diagnosis, cautious antibiotic use, and prompt treatment to prevent severe outcomes like shock and multiorgan failure.</p>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":" ","pages":"102936"},"PeriodicalIF":2.5,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142881141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of clinical characteristics, risk factors, and outcomes of patients infected with Bacteroides fragilis group at a tertiary care hospital in central China between 2017 and March 2024","authors":"Yongmei Li ,&nbsp;Jing Zhao ,&nbsp;Qiong Ma ,&nbsp;Junhong Xu ,&nbsp;Youhua Yuan ,&nbsp;Qi Zhang ,&nbsp;Wenjuan Yan ,&nbsp;Wenbo Xu ,&nbsp;Zhiyu Yang ,&nbsp;Gang Li ,&nbsp;Lan Gao ,&nbsp;Baoya Wang","doi":"10.1016/j.anaerobe.2024.102922","DOIUrl":"10.1016/j.anaerobe.2024.102922","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aimed to explore the epidemiology, clinical presentation, and risk factors for treatment failure in patients infected with various <em>Bacteroides fragilis</em> group (BFG) species.</div></div><div><h3>Methods</h3><div>We analyzed clinical and laboratory data from 178 patients infected with BFG who were admitted to our hospital between 2017 and 2024. We analyzed categorical data using Chi-square and Fisher exact tests, continuous variables using Student's <em>t</em>-tests or Mann–Whitney <em>U</em>-tests, and risk factors using multivariate logistic regression and Pearson's chi-squared tests.</div></div><div><h3>Results</h3><div><em>Bacteroides fragilis</em> (69.7 %) and <em>Bacteroides thetaiotaomicron</em> (16.9 %) were the most prevalent species among 178 BFG isolates. Most patients had polymicrobial infections, with the following pathogens isolated from concurrent samples: <em>Escherichia coli</em>, <em>Klebsiella</em> spp., and <em>Enterococcus</em> spp. Intra-abdominal sites were most frequently infected with BFG. Patients aged &gt;50 years and those who had intravascular catheters were infected with more other <em>Bacteroides</em> species than <em>B. fragilis</em>. Admission to an ICU, respiratory, renal and cardiovascular diseases, and chest drainage were associated more often with <em>B. thetaiotaomicron</em> than with <em>B. fragilis</em>. Gastrointestinal diseases, tracheal intubation, and ICU admission were associated more frequently with treatment failure in patients infected with <em>B. fragilis</em> whereas solid cancers, renal disease, multiple organ dysfunction syndrome, and tracheal intubation were more likely to be associated with treatment failure in patients infected with other <em>Bacteroides</em> spp.</div></div><div><h3>Conclusions</h3><div>The most prevalent BFG species in the patients were <em>B</em>. <em>fragilis</em> and <em>B</em>. <em>thetaiotaomicron</em>. The demographic characteristics of the patients, underlying diseases, and risk factors for poor clinical outcomes clearly differed among species.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"91 ","pages":"Article 102922"},"PeriodicalIF":2.5,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142708878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}