Comparison of Disc Diffusion and Sensititre® Broth Microdilution Methods for Antimicrobial Susceptibility Testing of Bacteroides fragilis Group clinical isolates following the French Guidelines.

IF 2.5 3区生物学 Q3 MICROBIOLOGY

Anaerobe Pub Date : 2025-05-06 DOI:10.1016/j.anaerobe.2025.102969

Gauthier Delvallez, Laure Diancourt, Julie Germond, Pascal Campagne, Bruno Dupuy

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引用次数: 0

Abstract

Objectives: This study aims to evaluate the concordance between the disc diffusion test (DDT) and custom-designed Sensititre® Broth Microdilution plates and to estimate their respective sensitivity (Se) and specificity (Sp) for antimicrobial susceptibility testing (AST) of Bacteroides fragilis group (BFG) clinical isolates.

Methods: AST was performed on 126 BFG isolates using both DDT and Sensititre® methods according to the 2024 CA-SFM guidelines. Cohen's kappa coefficients were used to assess concordance, while a Bayesian approach estimated Se and Sp with 95% confidence intervals (95% CIs) for amoxicillin-clavulanate (AMC), piperacillin-tazobactam (PTZ), imipenem (IPM), clindamycin (CLI), metronidazole (MTR), and moxifloxacin (MXF).

Results: Cohen's kappa coefficients with 95% CIs for AMC, PTZ, IPM, CLI, MTR, and MXF were 0.92 (0.85-1.00), 0.70 (0.56-0.83), 0.96 (0.92-1.00), 0.98 (0.94-1.00), 0.96 (0.89-1.00), and 0.70 (0.58-0.82), respectively. DDT demonstrated Se > 0.8 for all antibiotics, and Sp > 0.8 except for PTZ (0.51; 0.37-0.65) and CLI (0.71; 0.58-0.82). Sensititre® exhibited Se > 0.9 for all antibiotics except for PTZ (0.86; 0.70-0.99), and Sp > 0.9 except for MXF (0.88; 0.75-0.99).

Conclusions: DDT and Sensititre® Broth Microdilution demonstrated strong concordance for most antibiotics. Predictive values estimated using Bayesian model showed that Sensititre® offered the highest overall Se and Sp.

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光盘扩散法和Sensititre®微汤稀释法在法国易碎拟杆菌临床分离株药敏试验中的比较

目的：评价碟子扩散试验（DDT）与定制的Sensititre®微肉汤稀释板在脆弱拟杆菌（Bacteroides fragilis， BFG）临床分离株抗微生物药敏试验（AST）中的敏感性（Se）和特异性（Sp）。方法：根据2024 CA-SFM指南，使用DDT和Sensititre®方法对126株BFG分离株进行AST检测。采用Cohen’s kappa系数评估一致性，采用贝叶斯方法估计阿莫西林-克拉维酸酯（AMC）、哌拉西林-他唑巴坦（PTZ）、亚胺培南（IPM）、克林霉素（CLI）、甲硝唑（MTR）和莫西沙星（MXF）的Se和Sp的95%置信区间（95% ci）。结果：AMC、PTZ、IPM、CLI、MTR、MXF的95% ci Cohen’s kappa系数分别为0.92（0.85-1.00）、0.70（0.56-0.83）、0.96（0.92-1.00）、0.98（0.94-1.00）、0.96（0.89-1.00）、0.70（0.58-0.82）。滴滴涕对所有抗生素的Se >为0.8，除PTZ外，Sp >为0.8 (0.51；0.37-0.65)和CLI (0.71；0.58 - -0.82)。Sensititre®除PTZ (0.86；0.70-0.99), Sp > 0.9，除MXF (0.88；0.75 - -0.99)。结论：滴滴涕和Sensititre®微稀释肉汤对大多数抗生素具有很强的一致性。使用贝叶斯模型估计的预测值显示，Sensititre®提供最高的总体硒和Sp。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Anaerobe 生物-微生物学

CiteScore

5.20

自引率

8.70%

发文量

137

审稿时长

76 days

期刊介绍： Anaerobe is essential reading for those who wish to remain at the forefront of discoveries relating to life processes of strictly anaerobes. The journal is multi-disciplinary, and provides a unique forum for those investigating anaerobic organisms that cause infections in humans and animals, as well as anaerobes that play roles in microbiomes or environmental processes. Anaerobe publishes reviews, mini reviews, original research articles, notes and case reports. Relevant topics fall into the broad categories of anaerobes in human and animal diseases, anaerobes in the microbiome, anaerobes in the environment, diagnosis of anaerobes in clinical microbiology laboratories, molecular biology, genetics, pathogenesis, toxins and antibiotic susceptibility of anaerobic bacteria.