Anaerobe最新文献

{"title":"The identification of Finegoldia dalianensis sp. nov., isolated from the pus of the patient with skin abscess and genomic analysis of the strains belonging to Finegoldia genus","authors":"Yan Li ,&nbsp;Yan Wang ,&nbsp;Di Xiao ,&nbsp;Jing Wang ,&nbsp;Dong Jin","doi":"10.1016/j.anaerobe.2024.102913","DOIUrl":"10.1016/j.anaerobe.2024.102913","url":null,"abstract":"<div><h3>Objectives</h3><div>To comprehensively characterize a new species, named <em>Finegoldia dalianensis</em> sp. nov., isolated from the pus of a skin abscess from a patient and genomic analysis of the strains belonging to <em>Finegoldia</em> genus.</div></div><div><h3>Methods</h3><div>Strain LY240594^T was definitively characterized through phylogenetic, genomic, and biochemical approach. Extensive genomic comparisons, involving the genome of LY240594^T and those of 82 <em>Finegoldia</em> strains from GenBank, were instrumental in revealing genetic relationships within the <em>Finegoldia</em> genus.</div></div><div><h3>Results</h3><div>Strain LY240594 was initially identified as <em>F. magna</em> based on MALDI-TOF MS analysis, showing 99.7 % 16S rRNA gene sequences similarity with the type strain of <em>F. magna</em> CCUG 17636^T. However, there were 68.5 % similarity with dDDH method and 90.9 % similarity by ANI analysis respectively, between LY240594^T and the selected type strain, <em>F. magna</em> DSM 20470^T. Biochemical differences were also found between two strains. The ANI and genomic analysis of 82 <em>Finegoldia</em> sp. Strains and Strain LY240594 revealed that those strains could be categorized into at least three groups using a 95 % ANI threshold.</div></div><div><h3>Conclusion</h3><div>Comprehensive characterization supported the proposal of a new species within the genus <em>Finegoldia</em>, named <em>Finegoldia dalianensis</em> sp. nov. The type strain, LY240594^T (=GDMCC 1.4375^T = KCTC 25838^T), features 1938 genes and a G + C content of 31.8 mol%. Genomic comparisons and ANI studies elucidated substantial heterogeneity within the <em>Finegoldia</em> genus.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"90 ","pages":"Article 102913"},"PeriodicalIF":2.5,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142339808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization and antibiotic resistance of clinical Bacteroides and related genera from a tertiary care center in Türkiye","authors":"Mervenur Demir ,&nbsp;Jozsef Soki ,&nbsp;Elif Seren Tanrıverdi ,&nbsp;Ceren Özkul ,&nbsp;Bakhtiyar Mahmood ,&nbsp;Barış Otlu ,&nbsp;Gülşen Hazırolan ,&nbsp;ESCMID Study Group for Anaerobic Infections (ESGAI)","doi":"10.1016/j.anaerobe.2024.102912","DOIUrl":"10.1016/j.anaerobe.2024.102912","url":null,"abstract":"<div><h3>Objectives</h3><div>This study was conducted to measure the prevalence of antibiotic resistance, and corresponding resistance genes among <em>Bacteroides</em> and related genera in a tertiary hospital.</div></div><div><h3>Methods</h3><div>We examined 138 clinical strains of <em>Bacteroides, Phocaeicola</em> and <em>Parabacteroides</em> species isolated between July 2018 and June 2022. Antibiotic susceptibility tests were conducted using agar dilution. The <em>b</em><em>ft</em> gene and antibiotic resistance genes were targeted by real-time PCR.</div></div><div><h3>Results</h3><div>Resistance rates of all strains against ampicillin, cefoxitin, piperacillin-tazobactam, meropenem, imipenem, clindamycin, metronidazole, and tigecycline were 97.8 %, 28.3 %, 11.6 %, 7.9 %, 5.1 %, 47.8 %, 0 % and 4.3 %, respectively. Non-fragilis <em>Bacteroidales</em> spp. (NFB) exhibited lower susceptibility rates compared to <em>B. fragilis</em> for cefoxitin, clindamycin, and piperacillin-tazobactam. The prevalence of meropenem resistance was higher in <em>B. fragilis</em> (15.5 %) than in NFB (0 %). Among all strains, the rates of <em>cepA, cfxA, cfiA, ermF, ermG, ermB, nim, linA, mefA, msrSA, tetQ, tetX, tetX1</em> and <em>bft</em> genes were 42.8 %, 44.9 %, 8.7 %, 44.2 %, 10.9 %, 2.2 %, 0.7 %, 29.0 %, 17.4 %, 7.2 %, 76.1 %, 8.0 %, 37.7 % and 16.7 %, respectively. In five <em>B. fragilis</em> strains, insertion sequences [<em>IS1187</em>(n = 3), <em>ISBf6</em>(n = 1), <em>IS612B</em>(n = 1)] were detected in the upstream region of <em>cfiA</em>. <em>NimE</em> with <em>ISBf6</em> on plasmid pBFM29b was detected in one <em>B. fragilis</em> strain, intermediate to metronidazole (MIC = 16 μg/mL). <em>ErmF</em> was the most abundant gene responsible for clindamycin resistance. <em>TetQ</em> and <em>tetX1</em> genes exhibited a higher frequency in strains that were not susceptible to tigecycline (MIC ≥8 μg/ml).</div></div><div><h3>Conclusions</h3><div>Monitoring the resistance trends of <em>Bacteroides</em> and related genera is crucial given the observed resistance to all classes of antibiotics and the presence of various resistance mechanisms.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"90 ","pages":"Article 102912"},"PeriodicalIF":2.5,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142339809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comment on “Antimicrobial susceptibility of Bacteroides fragilis group organisms in Hong Kong, 2020–2021”","authors":"H. Marchandin,&nbsp;L. Dubreuil","doi":"10.1016/j.anaerobe.2024.102910","DOIUrl":"10.1016/j.anaerobe.2024.102910","url":null,"abstract":"","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"90 ","pages":"Article 102910"},"PeriodicalIF":2.5,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142279684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Pathogenic clostridia","authors":"Daniel Paredes-Sabja,&nbsp;Marjorie Pizarro-Guajardo,&nbsp;Joseph A. Sorg","doi":"10.1016/j.anaerobe.2024.102911","DOIUrl":"10.1016/j.anaerobe.2024.102911","url":null,"abstract":"","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"90 ","pages":"Article 102911"},"PeriodicalIF":2.5,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142239088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A subanalysis of Clostridium perfringens bloodstream infections from a 5-year retrospective nationwide survey (ITANAEROBY)","authors":"Nicholas Geremia ,&nbsp;Gianfranco Sanson ,&nbsp;Luigi Principe ,&nbsp;Roberta Maria Antonello ,&nbsp;Verena Zerbato ,&nbsp;Roberto Luzzati","doi":"10.1016/j.anaerobe.2024.102901","DOIUrl":"10.1016/j.anaerobe.2024.102901","url":null,"abstract":"<div><p><em>Clostridium perfingens</em> bloodstream infections (BSIs) can be associated with high mortality rates. We performed a subanalysis of all <em>C. perfringens</em> BSIs enrolled during a multicentric retrospective observational study (ITANAEROBY). Data were collected from January 2016 to December 2020. <em>C. perfringens</em> BSIs were 134 (134/1960, 6.8 %). The highest resistance rate was observed for clindamycin (26/120, 21.6 %), penicillin (11/71, 15.4 %) and metronidazole (14/131, 10.7 %). In conclusion, <em>C. perfringens</em> reduced susceptibility phenotype to first-line therapy.</p></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"90 ","pages":"Article 102901"},"PeriodicalIF":2.5,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clostridium perfringens antigens and challenges for development of vaccines against necrotic enteritis in poultry","authors":"Stefanie Bressan Waller ,&nbsp;Cleideanny Cancela Galvão ,&nbsp;Rafael Rodrigues Rodrigues ,&nbsp;Cleiderson de Lima Aguirres ,&nbsp;Pedro Henrique Dala Nora Quatrin ,&nbsp;Mariliana Luiza Ferreira Alves ,&nbsp;Marcos Roberto Alves Ferreira ,&nbsp;Fabricio Rochedo Conceição","doi":"10.1016/j.anaerobe.2024.102902","DOIUrl":"10.1016/j.anaerobe.2024.102902","url":null,"abstract":"<div><h3>Introduction</h3><p>Chickens with Necrotic Enteritis (NE), caused by <em>Clostridium perfringens</em>, exhibit acute and chronic symptoms that are difficult to diagnose, leading to significant economic losses. Vaccination is the best method for controlling and preventing NE. However, only two vaccines based on the CPA and NetB toxins have been commercialized, offering partial protection, highlighting the urgent need for more effective vaccines.</p></div><div><h3>Objective</h3><p>This review aimed to identify promising antigens for NE vaccine formulation and discuss factors affecting their effectiveness.</p></div><div><h3>Methods</h3><p>A systematic review using five scientific databases identified 30 eligible studies through the Rayyan tool, which were included for quality review.</p></div><div><h3>Results</h3><p>We identified 25 promising antigens, including CPA, NetB, FBA, ZMP, CnaA, FimA, and FimB, categorized by their role in disease pathogenesis. This review discusses the biochemical, physiological, and genetic traits of recombinant antigens used in vaccine prototypes, their expression systems, and immunization potential in chickens challenged with virulent <em>C. perfringens</em> strains. Market supply challenges, immunogenic potential, vaccine platforms, adjuvants, and factors related to vaccination schedules—such as administration routes, dosing intervals, and age at immunization—are also addressed. Additionally, the study notes that vaccine formulations tested under mild challenges may not offer adequate field-level protection due to issues replicating aggressive conditions, strain virulence loss, and varied methodologies.</p></div><div><h3>Conclusions</h3><p>An ideal NE vaccine should incorporate multiple antigens, molecular adjuvants, and delivery systems via <em>in ovo</em> and oral routes. The review underscores the challenges in developing and validating NE vaccines and the urgent need for a standardized protocol to replicate aggressive challenges accurately.</p></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"89 ","pages":"Article 102902"},"PeriodicalIF":2.5,"publicationDate":"2024-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142071803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High value-added chemical production through anaerobic codigestion of corn straw with a microbial consortium, cow manure and cow digestion solution","authors":"Farrukh Raza Amin ,&nbsp;Habiba Khalid ,&nbsp;Jingjing Wang ,&nbsp;Yaxiang Li ,&nbsp;Longxue Ma ,&nbsp;Wuxi Chen ,&nbsp;Yu Duan ,&nbsp;Yida Zhai ,&nbsp;Demao Li","doi":"10.1016/j.anaerobe.2024.102900","DOIUrl":"10.1016/j.anaerobe.2024.102900","url":null,"abstract":"<div><h3>Objectives</h3><p>This study investigated the codigestion of corn straw (CS) with cow manure (CM), cow digestion solution (CD), and a strain consortium (SC) for enhanced volatile fatty acid (VFA) production. The aims of this study were to develop a sustainable technique to increase VFA yields, examine how combining microbial reagents with CS affects VFA production by functional microorganisms, and assess the feasibility of improving microbial diversity through codigestion.</p></div><div><h3>Methods</h3><p>Batch experiments evaluated VFA production dynamics and microbial community changes with different combinations of CS substrates with CM, CD, and SC. Analytical methods included measuring VFAs by GC, ammonia and chemical oxygen demand (COD) by standard methods and microbial community analysis by 16S rRNA gene sequencing.</p></div><div><h3>Results</h3><p>Codigesting CS with the strain consortium yielded initial VFA concentrations ranging from 0.6 to 1.0 g/L, which were greater than those of the other combinations (0.05–0.3 g/L). Including CM, and CD further increased VFA production to 1.0–2.0 g/L, with the highest value of 2.0 g/L occurring when all four substrates were codigested. Significant ammonium reduction (194–241 mg/L to 29–37 mg/L) and COD reduction (3310–5250 mg/L to 730–1210 mg/L) were observed. Codigestion with CM and CD had greater Shannon diversity indices (3.19–3.24) than did codigestion with the other consortia (2.26). <em>Bacillota</em> dominated (96.5–99.6 %), with <em>Clostridiales</em> playing key roles in organic matter breakdown.</p></div><div><h3>Conclusions</h3><p>This study demonstrated the feasibility of improving VFA yields and harnessing microbial diversity through anaerobic codigestion of lignocellulosic and animal waste streams. Codigestion substantially enhanced VFA production, which was dominated by butyrate, reduced ammonium and COD, and enriched fiber-degrading and fermentative bacteria. These findings can help optimize codigestion for sustainable waste management and high-value chemical production.</p></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"89 ","pages":"Article 102900"},"PeriodicalIF":2.5,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141999256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxygen tolerance in anaerobes as a virulence factor and a health-beneficial property","authors":"Lyudmila Boyanova ,&nbsp;Liliya Boyanova ,&nbsp;Petyo Hadzhiyski ,&nbsp;Raina Gergova ,&nbsp;Rumyana Markovska","doi":"10.1016/j.anaerobe.2024.102897","DOIUrl":"10.1016/j.anaerobe.2024.102897","url":null,"abstract":"<div><p>Oxygen tolerance of anaerobes is a virulence factor, but can also be a beneficial property. Many species have evolved to tolerate or take advantage of the presence of low, especially nanaerobic (≤0.14 %) oxygen concentrations. Oxygen tolerance is genus-, species- and strain-dependent according to their protective mechanisms. It was better expressed in some pathogenic species such as <em>Bacteroides fragilis, Clostridioides difficile</em>, and <em>Clostridium perfringens,</em> as well as in <em>Akkermansia muciniphila</em> than in other potential probiotics such as <em>Alistipes, Blautia</em> and <em>Roseburia</em> spp. Different degrees of oxygen sensitivity were found between the strains of <em>Anaerostipes, Faecalibacterium,</em> and <em>Bifidobacterium</em> spp. Importantly, clostridial spores and anaerobes in biofilms are protected from oxidation. Rubrerythrins and flavodiiron proteins and two regulators (sigma factor B and PerR) contribute to <em>C. difficile</em> protection from reactive oxygen species (ROS). The frequent pathogen, <em>B. fragilis,</em> has numerous protective factors such as enzymes (catalase, superoxide dismutase, alkyl hydroperoxidase, thioredoxin peroxidase, and aerobic-type NrdAB ribonucleotide reductase), and nanaerobic respiration. Seven proteins confer strain-specific oxygen adaptation of <em>Faecalibacterium prausnitzii</em>. Oxygen tolerance protects anaerobes from ROS, shields their DNA and modulates gene expression. Furthermore, oxygen can induce mutations leading to antibiotic resistance as shown in <em>Prevotella melaninogenica</em>. Some <em>Faecalibacterium, Anaerostipes, Bifidobacterium,</em> and <em>Akkermansia</em> strains from the intestinal microbiota exhibiting oxygen tolerance may become next-generation probiotic candidates. Further studies are needed to reveal oxygen effects on more anaerobic species and strains, and the influence of oxygen on antibiotic resistance. More studies on oxygen-tolerant probiotic strains can be useful to optimize biotechnological methods.</p></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"89 ","pages":"Article 102897"},"PeriodicalIF":2.5,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141999257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical and microbiological characteristics of anaerobic bacteremia during 1994–2019: A Danish population-based cohort study","authors":"Kasper K. Mortensen ,&nbsp;Hans Linde Nielsen ,&nbsp;Kirstine K. Søgaard","doi":"10.1016/j.anaerobe.2024.102898","DOIUrl":"10.1016/j.anaerobe.2024.102898","url":null,"abstract":"<div><h3>Objectives</h3><p>Bacteremia with anaerobic bacteria is generally a marker of severe prognosis. However, population-based data is lacking. Our aim was to describe the epidemiology and the 30-day mortality rate of anaerobic bacteremia in a Danish population-based setting.</p></div><div><h3>Methods</h3><p>In this population-based cohort study, all first-time episodes of anaerobic bacteremia from the North Denmark Bacteremia Research Database during 1994–2019 were identified. Information on comorbidities, discharge diagnoses, and mortality was retrieved. 30-day mortality rates were calculated and a multivariate logistic regression analysis to identify risk factors for death was performed.</p></div><div><h3>Results</h3><p>1750 episodes with anaerobic bacteremia were identified, corresponding to an incidence rate of 12.5 per 100,000 inhabitants (increasing from 11.2 in 1994–2014 to 17.7 in 2015–2019). Of these episodes, a third were polymicrobial, and the majority (70 %) of patients had one or more comorbid conditions. Abdominal infection was the source of bacteremia in 61 % of patients, while it was unknown for 15 %. The most frequently isolated genera were <em>Bacteroides</em> (45 %), <em>Clostridium</em> (20 %) and <em>Fusobacterium</em> (6 %). The overall crude 30-day mortality rate was 27 %, but rates were even higher for patients of high age, with liver disease, and solid tumors. The odds ratio (OR) for 30-day mortality was 1.32 for <em>Clostridium</em> species, and 1.27 for polymicrobial bacteremia with aerobic bacteria.</p></div><div><h3>Conclusions</h3><p>The incidence rate of anaerobic bacteremia increased, and the 30-day mortality rate remained high during the study period. Multiple factors influence 30-day mortality rates, including high age, liver disease, solid tumor, polymicrobial bacteremia, and bacteremia with <em>Clostridium</em> species.</p></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"89 ","pages":"Article 102898"},"PeriodicalIF":2.5,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141987268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production of highly soluble and immuno-reactive recombinant flagellin protein of Clostridium chauvoei","authors":"Awadhesh Prajapati ,&nbsp;Roopa Anandamurthy Hemanth ,&nbsp;Mandira Ramakrishna Namrutha ,&nbsp;Suresh Bindu ,&nbsp;Revanaiah Yogisharadhya ,&nbsp;Nihar Nalini Mohanty ,&nbsp;Mohammed Mudassar Chanda ,&nbsp;Sathish Bhadravati Shivachandra","doi":"10.1016/j.anaerobe.2024.102899","DOIUrl":"10.1016/j.anaerobe.2024.102899","url":null,"abstract":"<div><h3>Objective</h3><p>Flagellin protein, an integral component of flagella, provides motility to several bacterial species and also acts as a candidate antigen in diagnostics and subunit vaccines. The bulk production of flagellin with retention of all conformational epitopes using recombinant protein technology is of paramount importance in the development of pathogen-specific immuno-assays and vaccines. We describe the production of highly soluble and immuno-reactive rFliA(C) protein of <em>Clostridium chauvoei,</em> a causative agent of blackleg or black quarter (BQ) affecting cattle and small ruminants worldwide. The bacterium is known to possess peritrichous flagella that provide motility and also act as a virulence factor with high protective antigenicity.</p></div><div><h3>Methods</h3><p>Upon sequence and structural analysis, a partial <em>fliA(C)</em> gene from <em>Clostridium chauvoei</em> was cloned and the recombinant mature protein with N- and C- terminal truncation was over-expressed as a His-tagged fusion protein (∼25 kDa) in <em>Escherichia coli</em>. Subsequently, rFliA(C) protein was purified by single-step affinity chromatography and characterized for its immuno-reactivity in laboratory animals, Western blot, and indirect-ELISA format.</p></div><div><h3>Results</h3><p>rFliA(C) was highly soluble and was purified in high quantity and quality. rFliA(C) elicited antigen-specific conformational polyclonal antibodies in rabbit and guinea pig models, as well as anti-<em>Clostridium chauvoei-</em>specific antibodies being specifically detected in BQ-vaccinated and convalescent sera of bovines in Western blot and in indirect-ELISA format. Further, no cross reactivity was noted with antibodies against major bovine diseases (e.g., foot-and-mouth disease, IBR, LSDV, hemorrhagic septicaemia, brucellosis, and leptospirosis).</p></div><div><h3>Conclusion</h3><p>The study indicated the production of conformational recombinant flagellin—rFliA(C)—antigen and its potential utility in development of diagnostics for detection of <em>Clostridium chauvoei-</em>specific antibodies in BQ-recovered and/or vaccinated animals.</p></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"89 ","pages":"Article 102899"},"PeriodicalIF":2.5,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141981547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}