Anaerobe最新文献

{"title":"Description of Anaerococcus kampingiae sp. nov., Anaerococcus groningensis sp. nov., Anaerococcus martiniensis sp. nov., and Anaerococcus cruorum sp. nov., isolated from human clinical specimens","authors":"K.E. Boiten,&nbsp;J. Meijer,&nbsp;E.M. van Wezel,&nbsp;A.C.M. Veloo","doi":"10.1016/j.anaerobe.2024.102935","DOIUrl":"10.1016/j.anaerobe.2024.102935","url":null,"abstract":"<div><h3>Objectives</h3><div>To improve the identification of anaerobic bacteria, the identity of clinical isolates which could not be identified using MALDI-TOF MS was assessed using whole genome sequencing (WGS) and in-house made main spectral profiles (MSPs) were created. Four novel <em>Anaerococcus</em> species, each represented by at least two isolates, were encountered.</div></div><div><h3>Methods</h3><div>The novelty of the isolates was confirmed by comparing the 16S rRNA gene sequences and the WGS with their closest relatives. Phylogenetic and phylogenomic relationships were determined using MEGA X and DSMZ TYGS. Biochemical features were determined and the clustering of the created MSPs was calculated. Possible clinical relevance was assessed.</div></div><div><h3>Results</h3><div>The novelty of the four different species was confirmed by the ANI value, and phylogenetic/phylogenomic clustering. Three of these species shared the same biochemical features, while one showed a different pattern. Only this latter species can be differentiated from other <em>Anaerococcus</em> spp. Remarkebly, six of the ten isolates were obtained from a positive blood culture, of which in five cases the bacterium was the only species encountered.</div></div><div><h3>Conclusions</h3><div>We propose to name these novel species: <em>Anaerococcus kampingiae</em> (ENR0874^T = DSM 117234^T, CCUG 77487^T (accession numbers PP192775/JBGMEF000000000)), <em>Anaerococcus groningensis</em> (ENR1011^T = DSM 117232^T, CCUG 77488^T (accession numbers PP192777/JBGMEG000000000)), <em>Anaerococcus martiniensis</em> (ENR0831^T = DSM 117233^T, CCUG 77486^T (accession numbers PP192776/JBGMEI000000000)), and <em>Anaerococcus cruorum</em> (ENR1039^T = DSM 117235^T, CCUG 77489^T (accession numbers PP192778/JBGMEH000000000)).</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"91 ","pages":"Article 102935"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142817073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Labelling of a live obligate anaerobe using fluorescent D-amino acids","authors":"Benjamin Lewis ,&nbsp;Adenrele Oludiran ,&nbsp;Ann Progulske-Fox ,&nbsp;William Dunn","doi":"10.1016/j.anaerobe.2025.102939","DOIUrl":"10.1016/j.anaerobe.2025.102939","url":null,"abstract":"<div><div>The probing of live bacteria via the incorporation of fluorescent D-amino acids (FDAAs) during peptidoglycan synthesis has been shown to be practical for visualizing both gram-positive and gram-negative bacterial species. This study demonstrates the reliability and applications of FDAA labelling for the fluorescent imaging of an obligate anaerobe.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"91 ","pages":"Article 102939"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142998883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genotypic and phenotypic diversity of carbapenem-resistant Bacteroides fragilis strains collected from different clinical origins","authors":"Yanyan Wang ,&nbsp;Juan Wen ,&nbsp;Binxin Guo ,&nbsp;Wenqi Zheng ,&nbsp;Junrui Wang","doi":"10.1016/j.anaerobe.2024.102924","DOIUrl":"10.1016/j.anaerobe.2024.102924","url":null,"abstract":"<div><h3>Objective</h3><div>Strains of carbapenem-resistant <em>Bacteroides fragilis</em> have frequently emerged in recent years. In China, data on the genotypic and phenotypic characteristics of these antimicrobial-resistant anaerobic bacteria are scarce. Thus, the aim of this study was to characterize clinical isolates of carbapenem-resistant <em>B. fragilis</em> collected from a tertiary hospital in China using whole genome sequencing (WGS), phenotypic susceptibility tests, and a biofilm formation assay.</div></div><div><h3>Methods</h3><div>We analyzed 49 <em>B. fragilis</em> strains with different antimicrobial resistance profiles. Antimicrobial susceptibility was determined using the agar dilution method and biofilm formation using a crystal violet assay. Genomic characteristics were analyzed using WGS, and the transcription level of <em>cfiA</em>, which is responsible for carbapenem resistance, was determined using quantitative reverse transcription polymerase chain reaction (PCR). Carbapenem-sensitive isolates were used as controls.</div></div><div><h3>Results</h3><div>All 49 <em>B. fragilis</em> isolates were biofilm producers and the percentage of carbapenem-resistant isolates was 42.86 % (21/49). The percentage of carbapenem-resistant isolates with medium-to-strong biofilm production ability was significantly lower than that of carbapenem-sensitive isolates (19.1 % vs. 88.9 %, <em>p</em> &lt; 0.01). None of the carbapenem-resistant <em>B. fragilis</em> isolates carried <em>bft</em>. In contrast, 53.6 % (15/28) of the carbapenem-sensitive isolates carried <em>bft</em>, and all of them were <em>fpn</em>(+). All carbapenem-resistant isolates (21/21, 100 %) harbored <em>cfiA</em> and its upstream insertion sequence (IS) element. Three isolates (BF058, BF059, and BF060) carried the IS613 element, which was not immediately adjacent upstream to <em>cfiA</em> but was separated by a 1000-kb sequence encoding <em>vatD</em>. The quantitative PCR assay results revealed the elevated expression of <em>cfiA</em> mRNA among carbapenem-resistant isolates, although the relative expression levels varied greatly among isolates. However, a significant correlation between the relative expression level of <em>cfiA</em> mRNA and phenotypic carbapenem resistance was observed.</div></div><div><h3>Conclusions</h3><div>Carbapenem-resistant <em>B. fragilis</em> isolates carried a low frequency of virulence-related genes and showed weaker biofilm formation ability compared with carbapenem-sensitive <em>B. fragilis</em> isolates. <em>CfiA</em> was the dominant mediator of carbapenem resistance in <em>B. fragilis</em>. This study was the first to identify the structural plasticity of the <em>cfiA</em>-IS element, emphasizing the diverse and complex evolution of carbapenem resistance in <em>B. fragilis</em>, which warrants further investigation.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"91 ","pages":"Article 102924"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunogenicity of a recombinant chimera composed of CROP domain segments from the hemorrhagic and lethal toxins of Paeniclostridium sordellii","authors":"Rafael Rodrigues Rodrigues ,&nbsp;Neida Conrad ,&nbsp;Marcos Roberto Alves Ferreira ,&nbsp;Clóvis Moreira Júnior ,&nbsp;Mariliana Luiza Ferreira Alves ,&nbsp;Pamela Aristimunho Sedrez ,&nbsp;Vitória Müller ,&nbsp;Alessandra Neis ,&nbsp;Miguel Andrade Bilhalva ,&nbsp;Cleideanny Cancela Galvão ,&nbsp;Fábio Pereira Leivas Leite ,&nbsp;Fabricio Rochedo Conceição","doi":"10.1016/j.anaerobe.2025.102938","DOIUrl":"10.1016/j.anaerobe.2025.102938","url":null,"abstract":"<div><div><em>Paeniclostridium sordellii</em> is responsible for severe infections in horses, cattle, and sheep, however, conventional vaccines exhibit limitations in production and immunogenicity. This study assessed the immunogenicity of a recombinant bacterin composed of a chimera (rQTcsHL) that combines segments from the lethal (TcsL) and hemorrhagic (TcsH) toxins in mice and sheep. Both immunized animal groups exhibited elevated levels of IgG, with the mice demonstrating moderate protection (&lt;50 %) against lethal challenges, comparable to that of the conventional vaccine. Further molecular optimization is essential to enhance its efficacy.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"91 ","pages":"Article 102938"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142963283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hypopituitarism secondary to internal carotid artery pseudoaneurysm following Lemierre's syndrome caused by Fusobacterium nucleatum","authors":"Christiane Gruetzmacher ,&nbsp;Marilena Nakaguma ,&nbsp;Raquel Soares Jallad ,&nbsp;Flora Ladeira Craveiro ,&nbsp;Ana Paula da Silva Tenorio ,&nbsp;Mario Padula ,&nbsp;Rafael Loch Batista","doi":"10.1016/j.anaerobe.2025.102942","DOIUrl":"10.1016/j.anaerobe.2025.102942","url":null,"abstract":"<div><div>Lemierre's syndrome (LS) is a rare complication of oropharyngeal infections, often causing sepsis, internal jugular vein thrombosis, and metastatic infections. We present a unique case of a 48-year-old male with LS complicated by an internal carotid artery pseudoaneurysm compressing the pituitary gland, resulting in panhypopituitarism. Diagnosis was confirmed via imaging and detection of <em>Fusobacterium nucleatum</em>. Treatment included antibiotics, anticoagulation, and hormone replacement. Although the patient recovered from pituitary deficiencies, growth hormone deficiency persisted. This case emphasizes the importance of recognizing endocrine dysfunctions in LS and highlights the need for multidisciplinary management to address vascular and neuroendocrine complications.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"92 ","pages":"Article 102942"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143122029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulatory networks: Linking toxin production and sporulation in Clostridioides difficile","authors":"Md Kamrul Hasan,&nbsp;Oluchi Alaribe,&nbsp;Revathi Govind","doi":"10.1016/j.anaerobe.2024.102920","DOIUrl":"10.1016/j.anaerobe.2024.102920","url":null,"abstract":"<div><div><em>Clostridioides difficile</em> has been recognized as an important nosocomial pathogen that causes diarrheal disease as a consequence of antibiotic exposure and costs the healthcare system billions of dollars every year. <em>C. difficile</em> enters the host gut as dormant spores, germinates into vegetative cells, colonizes the gut, and produces toxins TcdA and/or TcdB, leading to diarrhea and inflammation. Spores are the primary transmission vehicle, while the toxins A and B directly contribute to the disease. Thus, toxin production and sporulation are the key traits that determine the success of <em>C. difficile</em> as a pathogen. Both toxins and spores are produced during the late stationary phase in response to various stimuli. This review provides a comprehensive analysis of the current knowledge on the molecular mechanisms, highlighting the regulatory pathways that interconnect toxin gene expression and sporulation in <em>C. difficile</em>. The roles of carbohydrates, amino acids and other nutrients and signals, in modulating these virulence traits through global regulatory networks are discussed. Understanding the links within the gene regulatory network is crucial for developing effective therapeutic strategies against <em>C. difficile</em> infections, potentially leading to targeted interventions that disrupt the co-regulation of toxin production and sporulation.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"91 ","pages":"Article 102920"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New insights into the factors influencing methanogenic pathways in anaerobic digesters","authors":"Helena Rodrigues Oliveira ,&nbsp;Thuane Mendes Anacleto ,&nbsp;Fernanda Abreu ,&nbsp;Alex Enrich-Prast","doi":"10.1016/j.anaerobe.2024.102925","DOIUrl":"10.1016/j.anaerobe.2024.102925","url":null,"abstract":"<div><h3>Introduction</h3><div>Anaerobic digestion integrates waste treatment, energy generation, and nutrient recycling, producing methane mainly through acetoclastic (AM) and hydrogenotrophic methanogenesis (HM). Methanogenic pathway management can improve biogas productivity and quality. The balance between pathways is influenced by environmental and physicochemical conditions, with conflicting results on the effect of different factors often reported. This systematic review aims to clarify the influence of various parameters on methanogenic pathways in anaerobic digesters.</div></div><div><h3>Methods</h3><div>Literature search was conducted in the Web of Science and Scopus databases. The effects of different parameters on the predominant methanogenic pathway were evaluated using Kruskal-Wallis tests and Spearman's rank correlation.</div></div><div><h3>Results</h3><div>Thermophilic temperatures and high free ammonia nitrogen concentrations (&gt;300 mg L⁻¹) increase HM, with a strong combined effect of these variables. Conversely, under moderate temperature and ammonia concentrations, the primary feedstock influences the methanogenic pathway, with algae biomass, pig manure, and food industry wastewater showing the lowest contribution of hydrogenotrophic methanogens. pH effect varied with temperature, with acidic and alkaline pH favoring HM in mesophilic and thermophilic digesters, respectively. Furthermore, higher levels of volatile fatty acids (&gt;2000 mg L⁻¹), carbohydrates (&gt;10 g/L) and lipids (&gt;10 g/L) also appeared to favor HM over AM, while most metals – especially Cr, Se and W – promoted AM.</div></div><div><h3>Conclusion</h3><div>This study emphasizes the role of various factors in methanogenic pathway selection, highlighting the impact of previously overlooked parameters, such as inorganic elements and organic matter composition. These insights are essential for understanding the methanogenic pathway balance and optimizing biogas processes.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"91 ","pages":"Article 102925"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitin promotes equol production via n-acetylglucosamine in human fecal cultures","authors":"Miyuki Kodera ,&nbsp;Kohei Nakamura ,&nbsp;S. Yokoyama","doi":"10.1016/j.anaerobe.2024.102923","DOIUrl":"10.1016/j.anaerobe.2024.102923","url":null,"abstract":"<div><h3>Objectives</h3><div>Equol is a metabolite of isoflavone. Here, we investigated the effect of dietary fiber on equol-producing microbiota.</div></div><div><h3>Methods</h3><div>Human feces was anaerobically cultured in Gifu Anaerobic Medium (GAM) containing 1 mg/L daidzein supplemented with 1 % (w/v) dietary fiber (pectin, chitin, or resistant starch [RS]). The equol conversion rate, number of equol-producing bacteria, pH, short-chain fatty acids (SCFAs), and microbial composition were measured in fecal cultures.</div></div><div><h3>Results</h3><div>Equol conversion significantly increased by 2.7-fold in the chitin group compared with the control group. Conversely, the conversion rate decreased to one-tenth in the pectin group and one-third in the RS group. The number of equol-producing bacteria also significantly increased in the chitin group compared with the control group. Whereas pH values and acetate/propionate concentrations decreased in the pectin and RS groups compared with the control group, no significant differences were observed in the chitin group. Microbiota analysis indicated that the relative abundances of <em>Bifidobacterium</em> and <em>Lactobacillus</em> increased, whereas those of <em>Dorea</em>, <em>Lachnoclostridium</em>, and <em>Eggerthella</em> decreased in the pectin and RS groups. In contrast, these abundances remained stable in the chitin group. The relative abundances of <em>Adlercreutzia</em> and <em>Slackia</em> remained unchanged across all groups. <em>In vitro</em> cultures of <em>Eggerthella</em> sp. strain YY7918 and <em>Adlercreutzia equolifaicens</em> JCM14793 demonstrated that equol conversion from daidzein was stimulated by the addition of 0.5–1% (w/v) <span><em>n</em></span>-acetylglucosamine, a breakdown product of chitin, but not chitin itself.</div></div><div><h3>Conclusions</h3><div>Our findings suggest that equol production is stimulated by <span><em>n</em></span>-acetylglucosamine, derived from decomposed chitin, in certain equol-producing bacteria without affecting their growth.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"91 ","pages":"Article 102923"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142738151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A rapid genome-proteome approach to identify rate-limiting steps in the butyrate production pathway in probiotic Clostridium butyricum, CBM588","authors":"Liam Wood ,&nbsp;Bunmi B. Omorotionmwan ,&nbsp;Adam M. Blanchard ,&nbsp;Adam Dowle ,&nbsp;David Tooth ,&nbsp;Tom S. Bailey ,&nbsp;Ruth Griffin","doi":"10.1016/j.anaerobe.2025.102940","DOIUrl":"10.1016/j.anaerobe.2025.102940","url":null,"abstract":"<div><h3>Objectives</h3><div><em>Clostridium butyricum</em> ferments non-digested dietary fibre in the colon to produce butyric acid. Butyrate is a four-carbon, short-chain fatty acid (SCFA) which has multiple health benefits. Many microbial products of pharmaceutical or industrial interest, such as butyrate, are produced in low quantities due to rate-limiting steps in their metabolic pathway, including low abundance or low activity of one or more enzymes in the pathway. By identifying the former, appropriate enzymes can be over-expressed to increase product yields, however, methods to determine these enzymes are laborious. To improve butyrate production in <em>C. butyricum</em> probiotic strain, CBM588, a novel rapid genome-proteome approach was deployed.</div></div><div><h3>Methods</h3><div>First, whole genome sequencing was performed and the 8 genes involved in butyrate production identified on the chromosome. Second, the relative abundance of these enzymes was investigated by liquid chromatography-mass spectrometry (LC-MS) analysis of total cytosolic proteins from early stationary phase cultures.</div></div><div><h3>Results</h3><div>Phosphotransbutyrylase (Ptb), butyrate kinase (Buk) and crotonase (Crt) were found to be the least abundant. Over-expression episomally of the corresponding genes individually or of the <em>ptb-buk</em> bicistron led to significant increases in butyrate titre per density of culture from 10 to 24 h, compared to the wild type.</div></div><div><h3>Conclusions</h3><div>Our findings pave the way for over-expressing these genes chromosomally to generate a recombinant probiotic with improved butyrate production and potentially enhanced gut health properties.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"92 ","pages":"Article 102940"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A rare isolation of Parvimonas micra from cerebellar abscess in a patient with complex cyanotic heart disease.","authors":"Praveen Ravichandran, Ramit Kundu, Rakhi Biswas, Sathiaprabhu Anbazhagan","doi":"10.1016/j.anaerobe.2025.102943","DOIUrl":"https://doi.org/10.1016/j.anaerobe.2025.102943","url":null,"abstract":"<p><p>Parvimonas micra is a gram-positive coccus which is an obligate anaerobe. It is a commensal in the oropharyngeal cavity and the gastrointestinal tract. The genus Parvimonas has only a single species, P. micra. Although there are several reports of infections due to P. micra involving various body sites, cases of brain abscess due to this organism have been under-reported in the literature. Here we present a rare case of cerebellar abscess caused by P. micra in a nine-year-old boy with complex cyanotic heart disease.</p>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":" ","pages":"102943"},"PeriodicalIF":2.5,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}