Yuliang Zhao, Haijuan Qin, Yuhua Lyu, Song Yan, Huali Yu
{"title":"聚对苯二甲酸乙二醇酯微塑料急性暴露对厌氧氨氧化颗粒污泥性能的影响研究。","authors":"Yuliang Zhao, Haijuan Qin, Yuhua Lyu, Song Yan, Huali Yu","doi":"10.1016/j.anaerobe.2025.102984","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>Anaerobic ammonia oxidation is a new and efficient biological denitrification process, which has been used in practice. Research on the effect of microplastics (MPs) on anaerobic ammonia oxidation (anammox) process is limited.</div></div><div><h3>Topic</h3><div>In this study, the effects of short-term exposure of polyethylene terephthalate (PET) with different particle sizes for 12 h on anammox granular sludge (AnGS) and the metabolic mechanism of anaerobic ammonia oxidizing bacteria (AnAOB) were studied.</div></div><div><h3>Results</h3><div>The results showed that the acute exposure of anammox reactor to PET-MPs could promote the denitrification rate of the reactor. The enzyme activity of hydrazine dehydrogenase (HDH), the key enzyme of anammox reaction, was increased under acute exposure of different particle sizes of PET-MPs. PET-MPs with larger particle size had a stronger promoting effect on the treatment efficiency and HDH activity of the reactor. PET-MPs could stimulate AnAOB to secrete a large amount of extracellular polymeric substances (EPS). Metabonomic analysis showed that after the addition of PET-MPs, the abundance of 3-methoxytyramine was down-regulated, and the abundance of L-serine decreased. In the 13 μm PET-MPs group, the abundance of L-asparagine was up-regulated, changes in these metabolites affected various amino acid metabolism, proteolytic enzymes, and AnAOB biosynthesis.</div></div><div><h3>Implication</h3><div>This study provides valuable insights into the response characteristics and mechanism of the anammox process exposed to MPs, which may be helpful for the application of MPs in wastewater treatment.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"94 ","pages":"Article 102984"},"PeriodicalIF":2.6000,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Study on acute exposure of polyethylene terephthalate microplastics on the performance of anammox granular sludge\",\"authors\":\"Yuliang Zhao, Haijuan Qin, Yuhua Lyu, Song Yan, Huali Yu\",\"doi\":\"10.1016/j.anaerobe.2025.102984\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><div>Anaerobic ammonia oxidation is a new and efficient biological denitrification process, which has been used in practice. Research on the effect of microplastics (MPs) on anaerobic ammonia oxidation (anammox) process is limited.</div></div><div><h3>Topic</h3><div>In this study, the effects of short-term exposure of polyethylene terephthalate (PET) with different particle sizes for 12 h on anammox granular sludge (AnGS) and the metabolic mechanism of anaerobic ammonia oxidizing bacteria (AnAOB) were studied.</div></div><div><h3>Results</h3><div>The results showed that the acute exposure of anammox reactor to PET-MPs could promote the denitrification rate of the reactor. The enzyme activity of hydrazine dehydrogenase (HDH), the key enzyme of anammox reaction, was increased under acute exposure of different particle sizes of PET-MPs. PET-MPs with larger particle size had a stronger promoting effect on the treatment efficiency and HDH activity of the reactor. PET-MPs could stimulate AnAOB to secrete a large amount of extracellular polymeric substances (EPS). Metabonomic analysis showed that after the addition of PET-MPs, the abundance of 3-methoxytyramine was down-regulated, and the abundance of L-serine decreased. In the 13 μm PET-MPs group, the abundance of L-asparagine was up-regulated, changes in these metabolites affected various amino acid metabolism, proteolytic enzymes, and AnAOB biosynthesis.</div></div><div><h3>Implication</h3><div>This study provides valuable insights into the response characteristics and mechanism of the anammox process exposed to MPs, which may be helpful for the application of MPs in wastewater treatment.</div></div>\",\"PeriodicalId\":8050,\"journal\":{\"name\":\"Anaerobe\",\"volume\":\"94 \",\"pages\":\"Article 102984\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2025-07-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Anaerobe\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1075996425000472\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Anaerobe","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1075996425000472","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Study on acute exposure of polyethylene terephthalate microplastics on the performance of anammox granular sludge
Background
Anaerobic ammonia oxidation is a new and efficient biological denitrification process, which has been used in practice. Research on the effect of microplastics (MPs) on anaerobic ammonia oxidation (anammox) process is limited.
Topic
In this study, the effects of short-term exposure of polyethylene terephthalate (PET) with different particle sizes for 12 h on anammox granular sludge (AnGS) and the metabolic mechanism of anaerobic ammonia oxidizing bacteria (AnAOB) were studied.
Results
The results showed that the acute exposure of anammox reactor to PET-MPs could promote the denitrification rate of the reactor. The enzyme activity of hydrazine dehydrogenase (HDH), the key enzyme of anammox reaction, was increased under acute exposure of different particle sizes of PET-MPs. PET-MPs with larger particle size had a stronger promoting effect on the treatment efficiency and HDH activity of the reactor. PET-MPs could stimulate AnAOB to secrete a large amount of extracellular polymeric substances (EPS). Metabonomic analysis showed that after the addition of PET-MPs, the abundance of 3-methoxytyramine was down-regulated, and the abundance of L-serine decreased. In the 13 μm PET-MPs group, the abundance of L-asparagine was up-regulated, changes in these metabolites affected various amino acid metabolism, proteolytic enzymes, and AnAOB biosynthesis.
Implication
This study provides valuable insights into the response characteristics and mechanism of the anammox process exposed to MPs, which may be helpful for the application of MPs in wastewater treatment.
期刊介绍:
Anaerobe is essential reading for those who wish to remain at the forefront of discoveries relating to life processes of strictly anaerobes. The journal is multi-disciplinary, and provides a unique forum for those investigating anaerobic organisms that cause infections in humans and animals, as well as anaerobes that play roles in microbiomes or environmental processes.
Anaerobe publishes reviews, mini reviews, original research articles, notes and case reports. Relevant topics fall into the broad categories of anaerobes in human and animal diseases, anaerobes in the microbiome, anaerobes in the environment, diagnosis of anaerobes in clinical microbiology laboratories, molecular biology, genetics, pathogenesis, toxins and antibiotic susceptibility of anaerobic bacteria.