sordellipaeniclostridium出血性和致死性毒素的CROP结构域片段重组嵌合体的免疫原性。

IF 2.5 3区生物学 Q3 MICROBIOLOGY

Anaerobe Pub Date : 2025-01-08 DOI:10.1016/j.anaerobe.2025.102938

Rafael Rodrigues Rodrigues, Neida Conrad, Marcos Roberto Alves Ferreira, Clóvis Moreira Júnior, Mariliana Luiza Ferreira Alves, Pamela Aristimunho Sedrez, Vitória Müller, Alessandra Neis, Miguel Andrade Bilhalva, Cleideanny Cancela Galvão, Fábio Pereira Leivas Leite, Fabricio Rochedo Conceição

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引用次数: 0

摘要

sordellipaeniclostridium可引起马、牛和羊的严重感染；然而，传统疫苗在生产和免疫原性方面存在局限性。本研究在小鼠和绵羊中评估了由嵌合体（rQTcsHL）组成的重组细菌的免疫原性，该嵌合体结合了致命（TcsL）和出血性（TcsH）毒素的片段。两组免疫动物均表现出IgG水平升高，小鼠表现出中等程度的保护(

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Immunogenicity of a recombinant chimera composed of CROP domain segments from the hemorrhagic and lethal toxins of Paeniclostridium sordellii.

Paeniclostridium sordellii is responsible for severe infections in horses, cattle, and sheep, however, conventional vaccines exhibit limitations in production and immunogenicity. This study assessed the immunogenicity of a recombinant bacterin composed of a chimera (rQTcsHL) that combines segments from the lethal (TcsL) and hemorrhagic (TcsH) toxins in mice and sheep. Both immunized animal groups exhibited elevated levels of IgG, with the mice demonstrating moderate protection (<50 %) against lethal challenges, comparable to that of the conventional vaccine. Further molecular optimization is essential to enhance its efficacy.

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来源期刊

Anaerobe 生物-微生物学

CiteScore

5.20

自引率

8.70%

发文量

137

审稿时长

76 days

期刊介绍： Anaerobe is essential reading for those who wish to remain at the forefront of discoveries relating to life processes of strictly anaerobes. The journal is multi-disciplinary, and provides a unique forum for those investigating anaerobic organisms that cause infections in humans and animals, as well as anaerobes that play roles in microbiomes or environmental processes. Anaerobe publishes reviews, mini reviews, original research articles, notes and case reports. Relevant topics fall into the broad categories of anaerobes in human and animal diseases, anaerobes in the microbiome, anaerobes in the environment, diagnosis of anaerobes in clinical microbiology laboratories, molecular biology, genetics, pathogenesis, toxins and antibiotic susceptibility of anaerobic bacteria.