Molecular characterization and antibiotic resistance of clinical Bacteroides and related genera from a tertiary care center in Türkiye

IF 2.5 3区生物学 Q3 MICROBIOLOGY

Anaerobe Pub Date : 2024-09-24 DOI:10.1016/j.anaerobe.2024.102912

Mervenur Demir , Jozsef Soki , Elif Seren Tanrıverdi , Ceren Özkul , Bakhtiyar Mahmood , Barış Otlu , Gülşen Hazırolan , ESCMID Study Group for Anaerobic Infections (ESGAI)

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引用次数: 0

Abstract

Objectives

This study was conducted to measure the prevalence of antibiotic resistance, and corresponding resistance genes among Bacteroides and related genera in a tertiary hospital.

Methods

We examined 138 clinical strains of Bacteroides, Phocaeicola and Parabacteroides species isolated between July 2018 and June 2022. Antibiotic susceptibility tests were conducted using agar dilution. The bft gene and antibiotic resistance genes were targeted by real-time PCR.

Results

Resistance rates of all strains against ampicillin, cefoxitin, piperacillin-tazobactam, meropenem, imipenem, clindamycin, metronidazole, and tigecycline were 97.8 %, 28.3 %, 11.6 %, 7.9 %, 5.1 %, 47.8 %, 0 % and 4.3 %, respectively. Non-fragilis Bacteroidales spp. (NFB) exhibited lower susceptibility rates compared to B. fragilis for cefoxitin, clindamycin, and piperacillin-tazobactam. The prevalence of meropenem resistance was higher in B. fragilis (15.5 %) than in NFB (0 %). Among all strains, the rates of cepA, cfxA, cfiA, ermF, ermG, ermB, nim, linA, mefA, msrSA, tetQ, tetX, tetX1 and bft genes were 42.8 %, 44.9 %, 8.7 %, 44.2 %, 10.9 %, 2.2 %, 0.7 %, 29.0 %, 17.4 %, 7.2 %, 76.1 %, 8.0 %, 37.7 % and 16.7 %, respectively. In five B. fragilis strains, insertion sequences [IS1187(n = 3), ISBf6(n = 1), IS612B(n = 1)] were detected in the upstream region of cfiA. NimE with ISBf6 on plasmid pBFM29b was detected in one B. fragilis strain, intermediate to metronidazole (MIC = 16 μg/mL). ErmF was the most abundant gene responsible for clindamycin resistance. TetQ and tetX1 genes exhibited a higher frequency in strains that were not susceptible to tigecycline (MIC ≥8 μg/ml).

Conclusions

Monitoring the resistance trends of Bacteroides and related genera is crucial given the observed resistance to all classes of antibiotics and the presence of various resistance mechanisms.

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来自土耳其一家三级医疗中心的临床乳杆菌和相关菌属的分子特征和抗生素耐药性。

研究目的本研究旨在测量一家三甲医院中乳头疽菌属及相关菌属的抗生素耐药性及相应耐药基因的流行情况：我们检测了2018年7月至2022年6月期间分离的138株乳头菌属、噬菌体属和副乳头菌属的临床菌株。采用琼脂稀释法进行抗生素敏感性试验。通过实时 PCR 对 bft 基因和抗生素耐药基因进行靶向检测：所有菌株对氨苄西林、头孢西丁、哌拉西林-他唑巴坦、美罗培南、亚胺培南、克林霉素、甲硝唑和替加环素的耐药率分别为 97.8%、28.3%、11.6%、7.9%、5.1%、47.8%、0% 和 4.3%。与脆弱拟杆菌属相比，非脆弱拟杆菌属（NFB）对头孢西丁、林可霉素和哌拉西林-他唑巴坦的敏感率较低。脆弱拟杆菌对美罗培南的耐药率（15.5%）高于 NFB（0%）。在所有菌株中，cepA、cfxA、cfiA、ermF、ermG、ermB、nim、linA、mefA、msrSA、tetQ、tetX、tetX1 和 bft 基因的耐药率分别为 42.8 %、44.9 %、8.7 %、44.2 %、10.9 %、2.2 %、0.7 %、29.0 %、17.4 %、7.2 %、76.1 %、8.0 %、37.7 % 和 16.7 %。在 5 个 B. fragilis 菌株中，在 cfiA 的上游区域检测到了插入序列[IS1187（n = 3）、ISBf6（n = 1）、IS612B（n = 1）]。在一株对甲硝唑（MIC = 16 μg/mL）作用中等的脆弱拟杆菌（B. fragilis）菌株中检测到了质粒 pBFM29b 上带有 ISBf6 的 NimE。ErmF 是导致克林霉素耐药性的最大量基因。TetQ 和 tetX1 基因在对替加环素（MIC ≥8 μg/ml）不敏感的菌株中出现频率较高：结论：鉴于已观察到的对各类抗生素的耐药性以及各种耐药机制的存在，监测巴氏菌属及相关菌属的耐药趋势至关重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Anaerobe 生物-微生物学

CiteScore

5.20

自引率

8.70%

发文量

137

审稿时长

76 days

期刊介绍： Anaerobe is essential reading for those who wish to remain at the forefront of discoveries relating to life processes of strictly anaerobes. The journal is multi-disciplinary, and provides a unique forum for those investigating anaerobic organisms that cause infections in humans and animals, as well as anaerobes that play roles in microbiomes or environmental processes. Anaerobe publishes reviews, mini reviews, original research articles, notes and case reports. Relevant topics fall into the broad categories of anaerobes in human and animal diseases, anaerobes in the microbiome, anaerobes in the environment, diagnosis of anaerobes in clinical microbiology laboratories, molecular biology, genetics, pathogenesis, toxins and antibiotic susceptibility of anaerobic bacteria.