The identification of Finegoldia dalianensis sp. nov., isolated from the pus of the patient with skin abscess and genomic analysis of the strains belonging to Finegoldia genus

IF 2.5 3区生物学 Q3 MICROBIOLOGY

Anaerobe Pub Date : 2024-09-27 DOI:10.1016/j.anaerobe.2024.102913

Yan Li , Yan Wang , Di Xiao , Jing Wang , Dong Jin

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Abstract

Objectives

To comprehensively characterize a new species, named Finegoldia dalianensis sp. nov., isolated from the pus of a skin abscess from a patient and genomic analysis of the strains belonging to Finegoldia genus.

Methods

Strain LY240594^T was definitively characterized through phylogenetic, genomic, and biochemical approach. Extensive genomic comparisons, involving the genome of LY240594^T and those of 82 Finegoldia strains from GenBank, were instrumental in revealing genetic relationships within the Finegoldia genus.

Results

Strain LY240594 was initially identified as F. magna based on MALDI-TOF MS analysis, showing 99.7 % 16S rRNA gene sequences similarity with the type strain of F. magna CCUG 17636^T. However, there were 68.5 % similarity with dDDH method and 90.9 % similarity by ANI analysis respectively, between LY240594^T and the selected type strain, F. magna DSM 20470^T. Biochemical differences were also found between two strains. The ANI and genomic analysis of 82 Finegoldia sp. Strains and Strain LY240594 revealed that those strains could be categorized into at least three groups using a 95 % ANI threshold.

Conclusion

Comprehensive characterization supported the proposal of a new species within the genus Finegoldia, named Finegoldia dalianensis sp. nov. The type strain, LY240594^T (=GDMCC 1.4375^T = KCTC 25838^T), features 1938 genes and a G + C content of 31.8 mol%. Genomic comparisons and ANI studies elucidated substantial heterogeneity within the Finegoldia genus.

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鉴定从皮肤脓肿患者脓液中分离出的 Finegoldia dalianensis sp.

目的：从一名患者的皮肤脓肿脓液中分离出一个新物种，命名为Finegoldia dalianensis sp.nov.，并对属于Finegoldia属的菌株进行基因组分析：方法：通过系统发育、基因组和生化方法确定了菌株 LY240594T 的特征。通过对 LY240594T 基因组和 GenBank 中 82 株细金花属菌株的基因组进行广泛的基因组比较，有助于揭示细金花属内部的遗传关系：结果：根据 MALDI-TOF MS 分析，菌株 LY240594 与 F. magna 的模式菌株 CCUG 17636T 的 16S rRNA 基因序列相似度为 99.7%，被初步鉴定为 F. magna。然而，LY240594T 与所选模式菌株 F. magna DSM 20470T 的 dDDH 方法相似度为 68.5%，ANI 分析相似度为 90.9%。对 82 株 Finegoldia sp.菌株和菌株 LY240594 的 ANI 和基因组分析表明，以 95% 的 ANI 临界值为标准，这些菌株至少可分为三组：结论：综合分析结果表明，Finegoldia 属中有一个新种，命名为 Finegoldia dalianensis sp.模式菌株 LY240594T（=GDMCC 1.4375T =KCTC 25838T）有 1,938 个基因，G+C 含量为 31.8 摩尔%。基因组比较和 ANI 研究阐明了 Finegoldia 属内部的实质性异质性。

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来源期刊

Anaerobe 生物-微生物学

CiteScore

5.20

自引率

8.70%

发文量

137

审稿时长

76 days

期刊介绍： Anaerobe is essential reading for those who wish to remain at the forefront of discoveries relating to life processes of strictly anaerobes. The journal is multi-disciplinary, and provides a unique forum for those investigating anaerobic organisms that cause infections in humans and animals, as well as anaerobes that play roles in microbiomes or environmental processes. Anaerobe publishes reviews, mini reviews, original research articles, notes and case reports. Relevant topics fall into the broad categories of anaerobes in human and animal diseases, anaerobes in the microbiome, anaerobes in the environment, diagnosis of anaerobes in clinical microbiology laboratories, molecular biology, genetics, pathogenesis, toxins and antibiotic susceptibility of anaerobic bacteria.