Fructo-oligosaccharides promote butyrate production over citrus pectin during in vitro fermentation by colonic inoculum from pig

IF 2.5 3区生物学 Q3 MICROBIOLOGY

Anaerobe Pub Date : 2024-10-09 DOI:10.1016/j.anaerobe.2024.102919

Yanan Zhang , Chunlong Mu , Kaifan Yu , Yong Su , Erwin G. Zoetendal , Weiyun Zhu

{"title":"Fructo-oligosaccharides promote butyrate production over citrus pectin during in vitro fermentation by colonic inoculum from pig","authors":"Yanan Zhang , Chunlong Mu , Kaifan Yu , Yong Su , Erwin G. Zoetendal , Weiyun Zhu","doi":"10.1016/j.anaerobe.2024.102919","DOIUrl":null,"url":null,"abstract":"<div><h3>Objectives</h3><div>Fructo-oligosaccharide (FOS) and citrus pectin (CP) are soluble fibers with different chemical composition. However, their fermentation pattern in large intestine remains unclear.</div></div><div><h3>Methods</h3><div>An <em>in vitro</em> batch fermentation using colonic digesta from pigs as inoculum was employed to investigate the fermentation dynamics of FOS and CP. The monosaccharides and SCFAs contents were assayed by high-performance liquid chromatography and gas chromatography, respectively. And the microbiota community was assessed by 16S rRNA gene high-throughput sequencing.</div></div><div><h3>Results</h3><div>Both FOS and CP were degarded after 6 h, especially to a neglected level in FOS. FOS group showed higher abundances of butyrate-producing bacteria such as <em>Eubacterium rectale</em>, <em>Roseburia faecis</em> and <em>Coprococcus comes</em> and butyrate compared to CP. CP stimulated the growth of pectinolytic microbes <em>Lachnospira pectinoschiza</em>, succinate-producing bacteria <em>Succinivibrio dextrinosolvens</em>, succinate-utilizing bacteria <em>Phascolarctobacterium succinatutens</em> and the production of acetate and propionate compared to FOS. Moreover, the relative abundances of key enzymes (e.g. butyrate kinase) involving in butyrate formation via the butyrate kinase route were upregulated in the FOS group. And the key enzymes (e.g. acetyl-CoA synthetase) associated with propionate production through the succinate pathway were upregulated in the CP group.</div></div><div><h3>Conclusions</h3><div>FOS was preferred to ferment by butyrate-producing bacteria to yield a higher level of butyrate via the butyrate kinase pathway, while CP enhanced the cross-feeding of succinate-producing and succinate-utilizing bacteria to form propionate through the succinate pathway. These findings deepen our understanding on the fermentation characteristics of the soluble fibers, and also provide guidelines for fiber choice in precisely modulating the microbial composition and metabolism in large intestine.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"90 ","pages":"Article 102919"},"PeriodicalIF":2.5000,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Anaerobe","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1075996424001021","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Objectives

Fructo-oligosaccharide (FOS) and citrus pectin (CP) are soluble fibers with different chemical composition. However, their fermentation pattern in large intestine remains unclear.

Methods

An in vitro batch fermentation using colonic digesta from pigs as inoculum was employed to investigate the fermentation dynamics of FOS and CP. The monosaccharides and SCFAs contents were assayed by high-performance liquid chromatography and gas chromatography, respectively. And the microbiota community was assessed by 16S rRNA gene high-throughput sequencing.

Results

Both FOS and CP were degarded after 6 h, especially to a neglected level in FOS. FOS group showed higher abundances of butyrate-producing bacteria such as Eubacterium rectale, Roseburia faecis and Coprococcus comes and butyrate compared to CP. CP stimulated the growth of pectinolytic microbes Lachnospira pectinoschiza, succinate-producing bacteria Succinivibrio dextrinosolvens, succinate-utilizing bacteria Phascolarctobacterium succinatutens and the production of acetate and propionate compared to FOS. Moreover, the relative abundances of key enzymes (e.g. butyrate kinase) involving in butyrate formation via the butyrate kinase route were upregulated in the FOS group. And the key enzymes (e.g. acetyl-CoA synthetase) associated with propionate production through the succinate pathway were upregulated in the CP group.

Conclusions

FOS was preferred to ferment by butyrate-producing bacteria to yield a higher level of butyrate via the butyrate kinase pathway, while CP enhanced the cross-feeding of succinate-producing and succinate-utilizing bacteria to form propionate through the succinate pathway. These findings deepen our understanding on the fermentation characteristics of the soluble fibers, and also provide guidelines for fiber choice in precisely modulating the microbial composition and metabolism in large intestine.

查看原文本刊更多论文

在猪结肠接种物体外发酵过程中，果寡糖比柑橘果胶更能促进丁酸盐的产生。

目的：果寡糖（FOS）和柑橘果胶（CP）是具有不同化学成分的可溶性纤维。然而，它们在大肠中的发酵模式仍不清楚：方法：以猪的结肠消化液为接种物进行体外批量发酵，研究 FOS 和 CP 的发酵动态。高效液相色谱法和气相色谱法分别测定了单糖和 SCFAs 的含量。结果表明，FOS 和 CP 的单糖和 SCFAs 含量均有所下降：结果：6 小时后，两种底物中的单糖含量都有所下降，尤其是 FOS 中的单糖含量更低。与 CP 相比，FOS 表现出更高的丁酸菌（如直肠杆菌、粪蔷薇杆菌和来丁酸 Coprococcus）和丁酸盐的丰度。与 FOS 相比，CP 能刺激果胶溶解微生物 Lachnospira pectinoschiza、琥珀酸产生菌 Succinivibrio dextrinosolvens、琥珀酸利用菌 Phascolarctobacterium succinatutens 的生长，并促进乙酸盐和丙酸盐的产生。此外，通过丁酸激酶途径参与丁酸形成的关键酶（如丁酸激酶）的相对丰度在 FOS 组有所提高。而与通过琥珀酸途径产生丙酸盐有关的关键酶（如乙酰-CoA合成酶）在 CP 组中的相对丰度上调：结论：生产丁酸的细菌更喜欢 FOS 发酵，以通过丁酸激酶途径产生更高水平的丁酸，而 CP 则增强了生产琥珀酸的细菌和利用琥珀酸的细菌的交叉摄食，以通过琥珀酸途径形成丙酸。这些发现加深了我们对可溶性纤维发酵特性的理解，也为选择纤维以精确调节大肠微生物组成和代谢提供了指导。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Anaerobe 生物-微生物学

CiteScore

5.20

自引率

8.70%

发文量

137

审稿时长

76 days

期刊介绍： Anaerobe is essential reading for those who wish to remain at the forefront of discoveries relating to life processes of strictly anaerobes. The journal is multi-disciplinary, and provides a unique forum for those investigating anaerobic organisms that cause infections in humans and animals, as well as anaerobes that play roles in microbiomes or environmental processes. Anaerobe publishes reviews, mini reviews, original research articles, notes and case reports. Relevant topics fall into the broad categories of anaerobes in human and animal diseases, anaerobes in the microbiome, anaerobes in the environment, diagnosis of anaerobes in clinical microbiology laboratories, molecular biology, genetics, pathogenesis, toxins and antibiotic susceptibility of anaerobic bacteria.