Biochemical Genetics最新文献

Influence of FPGS rs1544105 and GGH rs3758149 Gene Polymorphisms on Methotrexate Pharmacogenetics.

IF 2.1 4区生物学

Biochemical Genetics Pub Date : 2025-03-06 DOI: 10.1007/s10528-025-11058-7

Andrea Giletti, Franca Lorenzelli, María Paz Menafra, Florencia Rivero, Mariana Lorenzo, Patricia Esperón

{"title":"Influence of FPGS rs1544105 and GGH rs3758149 Gene Polymorphisms on Methotrexate Pharmacogenetics.","authors":"Andrea Giletti, Franca Lorenzelli, María Paz Menafra, Florencia Rivero, Mariana Lorenzo, Patricia Esperón","doi":"10.1007/s10528-025-11058-7","DOIUrl":"https://doi.org/10.1007/s10528-025-11058-7","url":null,"abstract":"Methotrexate (MTX) pharmacogenetics has been extensively investigated due to the high inter-individual variability in response to treatment. This wide variability can lead to treatment discontinuation or even death. Several genes involved in the pharmacodynamics and pharmacokinetics of MTX have been studied. However, there are still no guidelines for pharmacogenetics-guided MTX dosing. The FPGS rs1544105 and GGH rs3758149 gene polymorphisms were genotyped and their allele frequencies were determined. Their associations with MTX treatment response and toxicity in Uruguayan adults with haematological malignancies receiving high-dose MTX were analyzed. A worldwide systematic review of the association of these gene polymorphisms with response and toxicity to high-dose MTX treatment was also conducted. The allele frequencies of FPGS rs1544105 were 0.54 and 0.46 (C and T, respectively), and of GGH rs3758149 were 0.77 and 0.23 (C and T, respectively). Several associations were found between toxicity (gastrointestinal, hepatic and hematological) and the FPGS rs1544105 T allele (p = 0.01, p < 0.001 and p = 0.04, respectively) and between mucositis and the FPGS TT genotype (p < 0.001). The GGH rs375814 TT genotype was associated with gastrointestinal and hepatic toxicity (p = 0.01 and p < 0.001, respectively). Both the FPGS rs1544105 C allele and the GGH rs3758149 TT genotype were associated with remission (p < 0.001 and p = 0.04, respectively). The systematic review identified 247 publications and finally included 17 research articles. Few consistent data were found due to the lack of homogeneity between study groups.","PeriodicalId":482,"journal":{"name":"Biochemical Genetics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143571826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pan-Cancer Analysis of ANO6 and Experimental Validation in Metastatic Melanoma.

IF 2.1 4区生物学

Biochemical Genetics Pub Date : 2025-03-05 DOI: 10.1007/s10528-025-11074-7

Yao An, Haoran Dong, Meishan Yan, Caixu Liu, Danfeng Hu, Qi Liu, Jingqiu Zhang, Xu Han, Zimeng Li, Minghui Xu, Li Chen, Quanzhi Zhang, Chunyan Gao

{"title":"Pan-Cancer Analysis of ANO6 and Experimental Validation in Metastatic Melanoma.","authors":"Yao An, Haoran Dong, Meishan Yan, Caixu Liu, Danfeng Hu, Qi Liu, Jingqiu Zhang, Xu Han, Zimeng Li, Minghui Xu, Li Chen, Quanzhi Zhang, Chunyan Gao","doi":"10.1007/s10528-025-11074-7","DOIUrl":"https://doi.org/10.1007/s10528-025-11074-7","url":null,"abstract":"Anoctamin 6 (ANO6) has been implicated in the oncogenicity of malignancies. However, pan-cancer analysis of ANO6 to fully explore its role in tumors has not been performed and little is reported on its role in melanoma. The ANO6 expression levels, clinical correlation, prognostic significance, mutational profiles, immune infiltration pattern, immune checkpoints, immunomodulatory genes, tumor heterogeneity, and molecular function were explored via systematic bioinformatics analysis and multiple public databases. Subsequently, the biological functions of ANO6 in the pulmonary metastasis of B16F10 melanoma cells in vivo were assessed by experimental validation. Our findings have demonstrated that ANO6 was highly expressed in most cancers and associated with poorer prognosis in cancer patients. A close relationship was observed between ANO6 expression level and clinicopathological characteristics, tumor immunity, and tumor heterogeneity. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses indicated that ANO6 was associated with immune response and regulated many cancer-related pathways. Furthermore, a melanoma pulmonary metastasis mice model showed that ANO6 was overexpressed in lung metastasis tissues compared with corresponding normal tissues. Collectively, ANO6 may serve as reliable biomarkers to predict the prognosis for diverse types of cancer and as a prospective marker for melanoma progression.","PeriodicalId":482,"journal":{"name":"Biochemical Genetics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143555533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decreased Complex I Activity in Blood lymphocytes Correlates with Idiopathic Pulmonary Fibrosis Severity.

IF 2.1 4区生物学

Biochemical Genetics Pub Date : 2025-03-04 DOI: 10.1007/s10528-025-11071-w

Emily Zifa, Sotirios Sinis, Anna-Maria Psarra, Andreas Mouikis, Aglaia Pozantzi, Konstantina Rossi, Foteini Malli, Ilias Dimeas, Paraskevi Kirgou, Konstantinos Gourgoulianis, Ourania S Kotsiou, Zoe Daniil

{"title":"Decreased Complex I Activity in Blood lymphocytes Correlates with Idiopathic Pulmonary Fibrosis Severity.","authors":"Emily Zifa, Sotirios Sinis, Anna-Maria Psarra, Andreas Mouikis, Aglaia Pozantzi, Konstantina Rossi, Foteini Malli, Ilias Dimeas, Paraskevi Kirgou, Konstantinos Gourgoulianis, Ourania S Kotsiou, Zoe Daniil","doi":"10.1007/s10528-025-11071-w","DOIUrl":"https://doi.org/10.1007/s10528-025-11071-w","url":null,"abstract":"Idiopathic pulmonary fibrosis (IPF) is a progressive interstitial lung disease linked to aging. Mitochondrial dysfunction in circulating T cells, often caused by disruption of mitochondrial DNA (mtDNA), may play a role in age-related conditions like IPF. In our previous study, we found high mtDNA mutational loads in blood lymphocytes from IPF patients, especially in regions critical for mtDNA expression. Since Complex I of the electron transport chain, partly encoded by mtDNA, is essential for energy production, we conducted a preliminary study on its activity. We found significantly reduced Complex I activity (p < 0.001) in lymphocytes from 40 IPF patients compared to 40 controls, which was positively correlated with lung function decline, specifically in functional vital capacity and diffusing capacity for carbon monoxide. These findings indicate that T cell mitochondrial dysfunction is associated with disease progression in IPF. Future work will explore the mechanisms linking T cell mitochondrial disruption with fibrosis, potentially uncovering new therapeutic targets.","PeriodicalId":482,"journal":{"name":"Biochemical Genetics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143555531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CircRNAs in Colorectal Cancer: Unveiling Their Roles and Exploring Therapeutic Potential.

IF 2.1 4区生物学

Biochemical Genetics Pub Date : 2025-03-03 DOI: 10.1007/s10528-025-11068-5

Yi-Han Ding, Xiao-Hang Song, Jing-Song Chen

引用次数: 0

miR-203 Alleviates Myocardial Damage Caused by Acute Coronary Syndrome by Inhibiting CA125.

IF 2.1 4区生物学

Biochemical Genetics Pub Date : 2025-02-28 DOI: 10.1007/s10528-025-11069-4

Yanfang Guo, Jinlin Li, Linhao Zhang

{"title":"miR-203 Alleviates Myocardial Damage Caused by Acute Coronary Syndrome by Inhibiting CA125.","authors":"Yanfang Guo, Jinlin Li, Linhao Zhang","doi":"10.1007/s10528-025-11069-4","DOIUrl":"https://doi.org/10.1007/s10528-025-11069-4","url":null,"abstract":"Acute coronary syndrome (ACS) is a significant contributor to cardiovascular mortality. Research has indicated that CA125 levels are linked to cardiovascular disease. This study aimed to explore the role of CA125 in ACS and its underlying mechanism. A retrospective study was conducted involving 34 healthy volunteers and 46 patients diagnosed with ACS. Clinical characteristics and CA125 expression were recorded and detected. Bioinformatics analysis was performed to identify miRNAs that regulate CA125. HL-1 cardiac muscle cells were subjected to oxygen-glucose deprivation/reoxygenation (OGD/R) to investigate the role of CA125 in myocardial injury. An ACS mice model was constructed to further explore the role of CA125 on ACS. The levels of serum creatinine, blood urea nitrogen, uric acid, high-sensitivity C-reactive protein, cystatin C, and white blood cells in ACS were markedly higher than those in healthy volunteers. CA125 was up-regulated in ACS and was a target of miR-203. Injection of miR-203 agomir reduced plaque deposition and vascular thrombosis in the coronary lumen, alleviating myocardial damage. Additionally, miR-203 inhibited myocardial apoptosis and inflammation responses induced by OGD/R and ACS. miR-203 can reduce the inflammatory response by inhibiting CA125 expression, thereby improving ACS symptoms and mitigating ACS-induced myocardial injury.","PeriodicalId":482,"journal":{"name":"Biochemical Genetics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143522275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SFRP4 Knockdown Attenuates Dsg2-Deficient Arrhythmogenic Cardiomyopathy by Down-Regulating TGF-β and Smad3. 敲除 SFRP4 可通过下调 TGF-β 和 Smad3 减轻 Dsg2 缺失型心律失常性心肌病

IF 2.1 4区生物学

Biochemical Genetics Pub Date : 2025-02-28 DOI: 10.1007/s10528-025-11052-z

Wei Li, Meixiang Wang, Zhongbao Ruan, Yin Ren, Li Zhu, Bo Zhang

{"title":"SFRP4 Knockdown Attenuates Dsg2-Deficient Arrhythmogenic Cardiomyopathy by Down-Regulating TGF-β and Smad3.","authors":"Wei Li, Meixiang Wang, Zhongbao Ruan, Yin Ren, Li Zhu, Bo Zhang","doi":"10.1007/s10528-025-11052-z","DOIUrl":"https://doi.org/10.1007/s10528-025-11052-z","url":null,"abstract":"Although secreted frizzled-related protein 4 (SFRP4) has been linked to the development of cardiovascular diseases; it is yet unknown how exactly it functions in arrhythmogenic cardiomyopathy (ACM) remains unclear. Data from the Gene Expression Omnibus (GEO) were used to identify genes that were differentially expressed and linked to ACM. A mouse model known as desmoglein 2 (Dsg2) knockout (Dsg2-/-) was employed to investigate ACM. Myocardial fibrosis was evaluated by histological analysis, while heart function was evaluated by echocardiography. Angiotensin II (Ang II) was used to stimulate cardiac fibroblasts (CFs) and cause a fibrotic phenotype. The ability of CFs to migrate was evaluate using a wound healing assay. Gene Set Enrichment Analysis (GSEA) was used to do an enrichment study of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. The levels of SFRP4, transforming growth factor beta receptor 2 (TGFBR2), TGF-β2, and Smad family member 3 (Smad3) were assessed using quantitative real-time PCR and Western blot. Our findings show that SFRP4 is highly expressed in Dsg2-/- mice. SFRP4 knockdown markedly reduced myocardial fibrosis, ventricular compliance, and cardiac dilation in Dsg2-/- mice. The level of SFRP4 was higher in CFs treated with Ang II, andSFRP4 inhibition markedly decreased the migration of Ang II-induced CFs. Moreover, SFRP4 activates the TGF-β signaling pathway, with SFRP4 knockdown resulting in a significant decrease in the expression levels of TGF-β2, TGFBR2, and Smad3 in Dsg2-/- mice. In summary, SFRP4 knockdown reduced cardiac fibrosis in ACM by inhibiting the TGF-β signaling pathway.","PeriodicalId":482,"journal":{"name":"Biochemical Genetics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143522411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Downregulation of RORl via STAT3 and P300 Promotes P38 Pathway- Dependent Lens Epithelial Cells Apoptosis in Age-Related Cataract. 通过 STAT3 和 P300 下调 RORl 可促进老年性白内障中 P38 通路依赖的晶状体上皮细胞凋亡

IF 2.1 4区生物学

Biochemical Genetics Pub Date : 2025-02-28 DOI: 10.1007/s10528-025-11067-6

Yue Zhang, Yuzhu Hu, Dongmei Su, Yanjiang Fu, Xiaoya Chen, Xiao Zhang, Shunfei Zheng, Xu Ma, Shanshan Hu

{"title":"Downregulation of RORl via STAT3 and P300 Promotes P38 Pathway- Dependent Lens Epithelial Cells Apoptosis in Age-Related Cataract.","authors":"Yue Zhang, Yuzhu Hu, Dongmei Su, Yanjiang Fu, Xiaoya Chen, Xiao Zhang, Shunfei Zheng, Xu Ma, Shanshan Hu","doi":"10.1007/s10528-025-11067-6","DOIUrl":"https://doi.org/10.1007/s10528-025-11067-6","url":null,"abstract":"Lens Epithelial Cells (LECs) apoptosis is a critical driving factor of age-related cataract (ARC), but the specific molecular mechanisms remain undefined. Herein, a novel target of ROR1 regulation was identified, the mechanism was elucidated by which ROR1 and its associated pathway proteins influence hydrogen peroxide (H2O2)-induced apoptosis of LECs in ARC. We found decreased ROR1 expression in human cataract lens capsules compared to normal ones, the trend was also observed in young and old mice. Experiments including CCK8, Hoechst 33,342 staining, and Western blot analysis confirmed that reduced ROR1 levels were linked to H2O2-induced apoptosis in HLEB3 cells. To investigate its effects on cell viability and apoptosis, we created a ROR1 interference plasmid and an overexpression plasmid. The overexpression of ROR1 effectively inhibited H2O2-induced apoptosis of HLEB3 cells while ROR1 knockdown lowered the viability and increased the apoptosis of HLEB3 cells. Additionally, increased P38 phosphorylation was identified as a contributor to lens epithelial cell apoptosis and ARC, with ROR1 influencing this through the phosphorylation of the P38. Similarly, the relationships between P300 and STAT3, upstream of ROR1, in apoptosis of LECs and ARC were explored, and it was found that P300 and STAT3 were negatively correlated with apoptosis of LECs and ARC. In addition, the double luciferase report showed that P300 and STAT3 synergistically up-regulated the expression of ROR1. Overall, this study demonstrates that the STAT3/ROR1/P38 pathway mitigates apoptosis of LECs in ARC progression, offering a novel strategy for ARC prevention and treatment in clinical settings.","PeriodicalId":482,"journal":{"name":"Biochemical Genetics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143522338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Pan- and Phylo-Genomic Analysis of Ideonella and Thermobifida Strains: Dissemination of Biodegradation Potential and Genomic Divergence.

IF 2.1 4区生物学

Biochemical Genetics Pub Date : 2025-02-25 DOI: 10.1007/s10528-025-11031-4

Arockiyajainmary Michealsamy, Saranya Jayapalan

{"title":"Comparative Pan- and Phylo-Genomic Analysis of Ideonella and Thermobifida Strains: Dissemination of Biodegradation Potential and Genomic Divergence.","authors":"Arockiyajainmary Michealsamy, Saranya Jayapalan","doi":"10.1007/s10528-025-11031-4","DOIUrl":"https://doi.org/10.1007/s10528-025-11031-4","url":null,"abstract":"Ideonella and Thermobifida were the most promising bacterial candidates for degrading plastic polymers. A comparative pan- and phylogenomic analysis of 33 Ideonella and Thermobifida strains was done to determine their plastic degradation potential, niche adaptation and speciation. Our study disclosed that more accessory genes in the strains showed phenotypic plasticity, according to the BPGA data. Pan and core genes were employed for the phylogenetic reconstruction. Pathway enrichment analyses scrutinized the functional roles of the core and adaptive-associated genes. KEGG annotation revealed that most genes were associated with the metabolism of amino acids and carbohydrates. The detailed COG analysis disclosed that approximately 40% of the pan genes performed metabolic functions. The unique gene pool consisted of genes chiefly involved in \"general function prediction\" and \"amino acid transport and metabolism\". Our in silico study revealed that these strains could assist in agronomic applications in the future since they devour nitrogen compounds and their central metabolic pathways are involved in amino acid metabolism. The rational selection of strains of Ideonella is far more effective at depolymerising plastics than Thermobifida. A greater number of unique genes, 1701 and 692, were identified for Ideonella sakaiensis 201-F6 and Thermobifida alba DSM-43795, respectively. Furthermore, we examined the singletons involved in xenobiotic catabolism. The unique singleton data were used to construct a supertree. To characterize the conserved patterns, we used SMART and MEME to identify domain and transmembrane regions in the unique protein sequences. Therefore, our study unraveled the genomic insights into the ecology-driven speciation of Ideonella and Thermobifida.","PeriodicalId":482,"journal":{"name":"Biochemical Genetics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143497535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complete Mitochondrial Genome and Phylogenetic Analysis of the Red Algae Chondracanthus tenellus (Rhodophyta, Gigartinales) from South Korea.

IF 2.1 4区生物学

Biochemical Genetics Pub Date : 2025-02-25 DOI: 10.1007/s10528-025-11063-w

Maheshkumar Prakash Patil, Hee-Eun Woo, Young-Ryun Kim, Jong-Oh Kim, Kyunghoi Kim

{"title":"Complete Mitochondrial Genome and Phylogenetic Analysis of the Red Algae Chondracanthus tenellus (Rhodophyta, Gigartinales) from South Korea.","authors":"Maheshkumar Prakash Patil, Hee-Eun Woo, Young-Ryun Kim, Jong-Oh Kim, Kyunghoi Kim","doi":"10.1007/s10528-025-11063-w","DOIUrl":"10.1007/s10528-025-11063-w","url":null,"abstract":"Red algae are widely used as a source of health-promoting bioactive compounds and dietary fibers in health foods. The identification and classification of red algal species based on morphological and molecular characteristics is challenging because of the similarity of the thallus and its high degree of plasticity and because complete mitochondrial genomes have only been reported for a few species. In this study, the complete mitochondrial genome sequencing of the red macroalga Chondracanthus tenellus (Harvey) (Hommersand et al., Hydrobiologia 260:105-120, 1993)) (Rhodophyta, Gigartinales) was performed for the first time. Additionally, we aimed to reconstruct the phylogenetic relationships of the species within the order Gigartinales using complete mitochondrial genome sequences. Genomic DNA was extracted, analyzed by whole-genome sequencing (WGS), and assembled using NOVOPlasty. The mitochondrial genome sequence was annotated, and both a genome map and a phylogenetic tree were constructed using maximum likelihood analysis. The mitochondrial genome was 25,928 bp in length, had strongly biased [AT] content (72.08%), and comprised 3 rRNAs, 23 tRNAs, and 24 protein-coding genes (PCGs). In comparison with the mitochondrial genome of other red algae, that of C. tenellus lacks rpl5 and rpl20. Based on a phylogenetic study of the complete mitochondrial genome, C. tenellus belongs to the family Gigartinaceae and is monophyletic with other species of the order Gigartinales. This is the first report of C. tenellus complete mitochondrial genome; its characteristics are consistent with those of other red algae. The study of genomic data will be beneficial for future comparative genomics, phylogenetics, and evolutionary studies.","PeriodicalId":482,"journal":{"name":"Biochemical Genetics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143490117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptome Analysis of Non-coding RNAs and mRNAs in the Dorsal Root Ganglion of Peripheral Nerve Injury-Induced Neuropathic Pain. 周围神经损伤诱发神经病理性疼痛的背根神经节非编码 RNA 和 mRNA 的转录组分析

IF 2.1 4区生物学

Biochemical Genetics Pub Date : 2025-02-24 DOI: 10.1007/s10528-025-11066-7

Wanxia Xiong, Yujia Liu, Xiaodong Ge, Jie Wang, Zhiyao Wang

{"title":"Transcriptome Analysis of Non-coding RNAs and mRNAs in the Dorsal Root Ganglion of Peripheral Nerve Injury-Induced Neuropathic Pain.","authors":"Wanxia Xiong, Yujia Liu, Xiaodong Ge, Jie Wang, Zhiyao Wang","doi":"10.1007/s10528-025-11066-7","DOIUrl":"https://doi.org/10.1007/s10528-025-11066-7","url":null,"abstract":"Maladaptive changes in gene expression at transcriptional level in dorsal root ganglia (DRGs) after nerve injury are critical for neuropathic pain genesis. Emerging evidence reveals the important role of non-coding RNAs (ncRNAs) in regulating gene transcription. Recent studies also have showed the contribution of ncRNAs to neuropathic pain. However, the expression profile of ncRNAs in the DRGs and potential regulatory mechanism in peripheral nerve injury-induced neuropathic pain are not fully clear. We used bCCI neuropathic pain model induced by chronic constriction injury of bilateral sciatic nerves to study the expression profile and potential functional mechanism of micro RNAs (miRNAs), long non-coding RNAs (lncRNAs), circular RNAs (circRNAs) and messenger RNA (mRNA) in the DRGs by RNA sequencing and bioinformatics analysis. A total of 47 miRNAs, 337 lncRNAs, 32 circRNAs, and 2269 mRNAs were differentially expressed (DE) in the DRGs of CCI mice 14 days after surgery. KEGG analysis demonstrated nociception-related signaling pathways were significantly enriched for DEncRNAs, including Rap1, Ras, and Hippo signaling pathway. GO analysis showed neuron related biological process, membrane related cell components, and binding related molecular functions were significantly enriched. The competing endogenous RNA (ceRNA) regulatory network of DEmiRNA-DEmRNA, DElncRNA-DEmRNA, and DEcircRNA-DEmiRNA existed in the DRGs of mice with neuropathic pain induced by peripheral nerve injury. In addition, 81 pain-related DE genes had protein-protein interactions (PPI) with each other. Our findings indicated that ncRNAs are involved in the development of peripheral nerve injury-induced neuropathic pain. DEncRNAs may provide us with a new perspective in chronic neuropathic pain research and may become a potential target for pain treatment.","PeriodicalId":482,"journal":{"name":"Biochemical Genetics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143490123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0