Biochemical Genetics最新文献_第10页

TMEM44 as a Novel Prognostic Marker for Hepatocellular Carcinoma is Associated with Tumor Invasion, Migration, and Apoptosis. TMEM44作为一种新的肝细胞癌预后标志物与肿瘤侵袭、迁移和凋亡相关

IF 2.1 4区生物学

Biochemical Genetics Pub Date : 2025-06-20 DOI: 10.1007/s10528-025-11161-9

Zhipeng Wu, Bingbing Qiu, Shuiqiu Liu, Yiran Hong, Liang Sun, Xiangbao Yin, Yonge Huang, Zhendong Chen

{"title":"TMEM44 as a Novel Prognostic Marker for Hepatocellular Carcinoma is Associated with Tumor Invasion, Migration, and Apoptosis.","authors":"Zhipeng Wu, Bingbing Qiu, Shuiqiu Liu, Yiran Hong, Liang Sun, Xiangbao Yin, Yonge Huang, Zhendong Chen","doi":"10.1007/s10528-025-11161-9","DOIUrl":"10.1007/s10528-025-11161-9","url":null,"abstract":"The transmembrane protein (TMEM) family represents a class of integral membrane proteins that traverse the lipid bilayer and play pivotal roles in diverse cellular processes. Functioning as molecular channels or regulators, TMEM proteins mediate selective transmembrane transport and have been implicated in the pathogenesis and progression of multiple malignancies. However, the biological significance of TMEM44 in hepatocellular carcinoma (HCC) remains largely unexplored. Through integrated bioinformatics analysis of clinical datasets, we identified significant upregulation of TMEM44 in HCC tissues, which correlated with adverse prognostic indicators. This overexpression was further validated at both protein and transcriptional levels via Western blotting and quantitative reverse transcription PCR (RT-qPCR) in HCC-derived cell lines and patient specimens. Functional characterization employing loss-of-function approaches in HCCLM3 and Huh-7 cell models demonstrated that TMEM44 silencing markedly attenuated malignant phenotypes: Transwell migration assays and wound healing analysis revealed impaired cellular motility, while 5-ethynyl-2'-deoxyuridine (EdU) incorporation assays showed suppressed proliferation. Furthermore, flow cytometric analysis indicated induced cell cycle arrest at the G0/G1 phase and enhanced apoptotic susceptibility upon TMEM44 knockdown. Collectively, our results identify TMEM44 as a critical oncogenic regulator in HCC, underscoring its dual potential as a prognostic biomarker and a therapeutic target for precision oncology.","PeriodicalId":482,"journal":{"name":"Biochemical Genetics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144332154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Study of the Poly (ADP-ribose) Polymerase-1 Gene as a Promotor of Inflammation-Driven Colorectal Carcinoma. Poly （adp -核糖）聚合酶-1基因作为炎症驱动的结直肠癌启动子的分子研究。

IF 2.1 4区生物学

Biochemical Genetics Pub Date : 2025-06-19 DOI: 10.1007/s10528-025-11159-3

Eman Hamdey Hamed Aziz, Alshimaa Magdy, Moustafa Abo Zaid, Raymonde Hanna Assaf

{"title":"Molecular Study of the Poly (ADP-ribose) Polymerase-1 Gene as a Promotor of Inflammation-Driven Colorectal Carcinoma.","authors":"Eman Hamdey Hamed Aziz, Alshimaa Magdy, Moustafa Abo Zaid, Raymonde Hanna Assaf","doi":"10.1007/s10528-025-11159-3","DOIUrl":"10.1007/s10528-025-11159-3","url":null,"abstract":"Colorectal cancer (CRC) is the third most common cancer and the second leading cause of cancer-related deaths worldwide. Chronic inflammation is a risk factor for various cancers, including CRC. However, the specific mechanisms by which inflammation contributes to cancer development are not fully understood. Our study assessed PARP1 and NF-κB mRNA expression in different stages of CRC, aiming to elucidate their roles in inflammation-driven CRC pathogenesis and define their stage-specific expression patterns. The study involved 35 CRC tissue samples and a control group of 25 samples from the healthy margins of colon cancer. PARP1 and NF-κB mRNA levels were determined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) in tumor tissue samples, as well as the adjacent part of normal tissue. Our results revealed that PARP1 and NF-κB/p50 gene expression was significantly higher in CRC vs. control. Furthermore, there was a positive correlation between PARP1 and NF-κB/p50 mRNA expression levels. PARP1 was found to be responsible for 14.5% of the change in NF-κB/p50. Both PARP1 and NF-κB/p50 had high accuracy in the diagnosis of CRC with AUC = 0.905 and 0.956, respectively. This study revealed the overexpression of PARP1 and NF-κB genes in CRC cases, which suggests that the use of PARP1 inhibitors and anti-inflammatory drugs could be effective in CRC treatment. PARP1/NF-κB showed preliminary associations with CRC that merit diagnostic evaluation in larger studies. Our data suggest that PARP1 and NF-κB expression may complement CEA in characterizing CRC biology though future studies must determine whether these markers have independent prognostic value.","PeriodicalId":482,"journal":{"name":"Biochemical Genetics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144332153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Early Detection of Sex and Cannabinoid Chemotypes in Cannabis sativa: SCAR Marker Validation in Indigenous Indian Germplasm. 大麻性别和大麻素化学型的早期检测：印度土著种质的SCAR标记验证。

IF 2.1 4区生物学

Biochemical Genetics Pub Date : 2025-06-19 DOI: 10.1007/s10528-025-11166-4

Yash Pandey, Trishna Chaturvedi, Nivedita Singh, Anil Kumar Gupta, Karuna Shanker, Gunjan Tiwari

{"title":"Early Detection of Sex and Cannabinoid Chemotypes in Cannabis sativa: SCAR Marker Validation in Indigenous Indian Germplasm.","authors":"Yash Pandey, Trishna Chaturvedi, Nivedita Singh, Anil Kumar Gupta, Karuna Shanker, Gunjan Tiwari","doi":"10.1007/s10528-025-11166-4","DOIUrl":"https://doi.org/10.1007/s10528-025-11166-4","url":null,"abstract":"Cannabis sativa is a versatile crop of immense industrial and medicinal value, cultivated for its fiber, seeds, and especially for its bioactive cannabinoids, yet early identification of sex and chemotype remains challenging due to delayed morphological differentiation and limited cannabinoid accumulation during early growth. This study validated reproducible DNA markers for early selection in Indian cannabis germplasm. For sex determination, i.e., three Sequence Characterized Amplified Region (SCAR) markers-SCAR119, SCAR323, and the Male-Associated DNA Clone 2 (MADC2), were evaluated. The MADC2 marker reliably amplified a 390 bp fragment in male plants, whereas most female plants exhibited 560 bp and 870 bp bands. This strong correlation between MADC2 genotypes and phenotypic sex underscores its utility for early sex identification, which is critical for maximizing cannabinoid yield by preventing unwanted pollination. In parallel, chemotype-specific markers were assessed to predict cannabinoid profiles. Using high-performance liquid chromatography (HPLC), plants were classified into three chemotypes based on THCA: CBDA ratios: THC-dominant, intermediate, and CBD-dominant. The dominant marker D589 was associated with THC biosynthesis, while the co-dominant marker B1080/B1192 provided refined differentiation between chemotypes by detecting allelic variations in cannabinoid synthase genes. Cloning and sequence analysis of these markers revealed significant similarity to known genomic regions involved in cannabinoid production. In summary, the validated SCAR markers enable early and accurate identification of sex and cannabinoid chemotype in Cannabis sativa. These tools offer substantial benefits for marker-assisted breeding, regulatory compliance (e.g., maintaining THC levels below 0.3% for hemp), and optimizing cultivation practices for both industrial and pharmaceutical applications in India.","PeriodicalId":482,"journal":{"name":"Biochemical Genetics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144323995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondrial Perspective on Species Complexes and Evolutionary Dynamics Within Genus Channa. 线粒体视角下的物种复合体和进化动力学。

IF 2.1 4区生物学

Biochemical Genetics Pub Date : 2025-06-18 DOI: 10.1007/s10528-025-11157-5

Sonakshi Modeel, Meenu Chaurasia, Sneha Siwach, Padma Dolkar, Rakesh Kumar Negi, Ram Krishan Negi

{"title":"Mitochondrial Perspective on Species Complexes and Evolutionary Dynamics Within Genus Channa.","authors":"Sonakshi Modeel, Meenu Chaurasia, Sneha Siwach, Padma Dolkar, Rakesh Kumar Negi, Ram Krishan Negi","doi":"10.1007/s10528-025-11157-5","DOIUrl":"https://doi.org/10.1007/s10528-025-11157-5","url":null,"abstract":"The genus Channa, commonly known as Snakeheads comprises a diverse variety of species that hold great significance in the commercial sectors. Their complexity and genetic variety show how adaptable they are to different environmental settings and shed light on their evolutionary history. To delve into the genetic intricacies of the genus Channa, we analyzed mitochondrial genetic diversity using 1372 COI sequences obtained from the Barcode of Life Data System (BOLD) database. The metadata and phylogenetic analysis revealed the presence of species complex within C. gachua and C. marulius, suggesting the potential existence of intra and inter-clades within one species group. Further, we selected four ecologically and economically important taxonomic groups to study their haplotype diversity and genetic differentiation. These species/taxonomic groups include C. striata, C. punctata, gachua species complex, and marulius species complex. The analysis indicated a substantial level of genetic differentiation and haplotype diversity in species groups indicating high gene flow within populations. Mitochondrial introgression and species complexes account for a significant section of errors in DNA barcodes, which are two of the primary challenges associated with employing DNA barcoding to identify species. Highlighting these challenges and ongoing uncertainties in specific taxonomic groups of genus Channa, the study argues that the efficacy of DNA barcoding and the genetic integrity of wild variation may be weakened when speciation results in the establishment of numerous cryptic taxa in a species complex.","PeriodicalId":482,"journal":{"name":"Biochemical Genetics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144323996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: RP13 516M14.1 Regulates Autophagy Through miR 429 via Both a ceRNA Network and Direct Interaction. RP13 516M14.1通过miR 429通过ceRNA网络和直接相互作用调节自噬。

IF 2.1 4区生物学

Biochemical Genetics Pub Date : 2025-06-17 DOI: 10.1007/s10528-025-11165-5

Zhijian Yang, Anyu Zeng, Baoxi Yu, Chao Xie, Weiwen Zhu, Hailong Liu, Cheng Gu, Ming Fu

引用次数: 0

Predicting Risk of Lymph Node Metastasis in Papillary Thyroid Cancer Using miRNAs and Clinicopathological Features. 应用mirna和临床病理特征预测甲状腺乳头状癌淋巴结转移风险。

IF 2.1 4区生物学

Biochemical Genetics Pub Date : 2025-06-17 DOI: 10.1007/s10528-025-11168-2

Penbe Gul Ergun, Cagatay Aydogan, Ayse Begum Ceviz, Gonca Candan, Sebnem Batur, Hulya Yilmaz Aydogan, Oguz Ozturk, Tulin Ozturk

{"title":"Predicting Risk of Lymph Node Metastasis in Papillary Thyroid Cancer Using miRNAs and Clinicopathological Features.","authors":"Penbe Gul Ergun, Cagatay Aydogan, Ayse Begum Ceviz, Gonca Candan, Sebnem Batur, Hulya Yilmaz Aydogan, Oguz Ozturk, Tulin Ozturk","doi":"10.1007/s10528-025-11168-2","DOIUrl":"https://doi.org/10.1007/s10528-025-11168-2","url":null,"abstract":"This study investigates potential prognostic values in models created with clinicopathological features and expression profiles of miRNAs known for their critical roles in papillary thyroid carcinoma (PTC) for lymph node metastasis (LNM) in PTC cases. Forty-seven lymph node-metastatic PTC patients and 46 non-metastatic patients were included. Using RT-PCR, miR-21, miR-146b, miR-221, and miR-222 expression was analyzed in carcinoma tissues and metastatic lymph nodes of the same PTC patients and carcinoma tissues of non-metastatic PTC patients. MiR-146b (p < 0.001) and miR-221 (p = 0.009) were overexpressed in metastatic PTCs compared to non-metastatic PTCs. The miRNA expression profiles were similar in the lymph nodes and primary cancer of metastatic PTCs. In the presence of larger tumors (> 1 cm) in metastatic PTCs, miR-146b and miR221 were overexpressed in the tumor tissue (p = 0.036), while miR-222 was overexpressed in metastatic lymph nodes (p = 0.035). miR-146b was also upregulated in lack of peritumoral lymphocyte infiltration (p = 0.006). In conclusion, our findings suggest that the overexpression of miR-146b and miR-221 in PTC tissues may be associated with lymph node metastasis and a poorer prognosis. Furthermore, the presence of larger tumors and increased levels of intratumoral fibrosis in non-metastatic PTC patients could indicate a poor prognosis.","PeriodicalId":482,"journal":{"name":"Biochemical Genetics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144315712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of the Antibacterial Effectiveness of Hybrid Recombinant Bacteriocins Circular Enterocin and Pyocin L1 Derived from Pseudomonas aeruginosa and Enterococcus faecalis. 铜绿假单胞菌和粪肠球菌混合重组细菌素环状肠素和Pyocin L1的抑菌效果研究。

IF 2.1 4区生物学

Biochemical Genetics Pub Date : 2025-06-13 DOI: 10.1007/s10528-025-11149-5

Lina Jamil Abdel-Hafez, Mohamed Salah Basiouny, Islam Yousif Mostafa, Rehab Moustafa, Khaled Abuelhaded, Haytham Mohamed Abd-Elhalim, Abanoub A S Shaker, Hassan Elsayed, Olfat Salem, Amir Mahfouz Mokhtar Sarguos, Ahmed Hassan Ibrahim Faraag

{"title":"Investigation of the Antibacterial Effectiveness of Hybrid Recombinant Bacteriocins Circular Enterocin and Pyocin L1 Derived from Pseudomonas aeruginosa and Enterococcus faecalis.","authors":"Lina Jamil Abdel-Hafez, Mohamed Salah Basiouny, Islam Yousif Mostafa, Rehab Moustafa, Khaled Abuelhaded, Haytham Mohamed Abd-Elhalim, Abanoub A S Shaker, Hassan Elsayed, Olfat Salem, Amir Mahfouz Mokhtar Sarguos, Ahmed Hassan Ibrahim Faraag","doi":"10.1007/s10528-025-11149-5","DOIUrl":"https://doi.org/10.1007/s10528-025-11149-5","url":null,"abstract":"This study investigates the antibacterial effectiveness of hybrid recombinant bacteriocins, specifically circular enterocin and pyocin L1, derived from Pseudomonas aeruginosa and Enterococcus faecalis. Novel hybrid variations, such as H-PyCir, were produced by combining, expressing, and isolating the genes responsible for these bacteriocins. The hybrid bacteriocins were purified and tested against a wide range of bacterial strains, demonstrating strong antibacterial activity against both Gram-positive and Gram-negative bacteria. The results reveal that hybrid bacteriocins, particularly H-PyCir, exhibit significantly enhanced antibacterial efficacy compared to their individual parent bacteriocins. Techniques such as phylogenetic analysis, plasmid construction, 3D homology modeling, and in silico docking were employed to investigate the structural and functional properties of these hybrids, providing insights into their mechanisms of action. The study also conducted well diffusion assays, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) tests, which confirmed the superior antimicrobial activity of H-PyCir, with the lowest MIC and MBC values observed across all tested strains. SDS-PAGE analysis further validated the successful cloning, expression, and purification of the hybrid bacteriocins, indicating high purity and correct molecular weights. These results highlight the potential of hybrid bacteriocins as promising alternatives to traditional antibiotics, offering a novel therapeutic approach to combat antibiotic resistance.","PeriodicalId":482,"journal":{"name":"Biochemical Genetics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144281932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessing Genetic Variation and Population Connectivity in Wallago attu: A Call for Enhanced Conservation Measures. 评估Wallago attu的遗传变异和种群连通性：加强保护措施的呼吁。

IF 2.1 4区生物学

Biochemical Genetics Pub Date : 2025-06-12 DOI: 10.1007/s10528-025-11156-6

Taqwa Safdar, Khalid Abbas, Cedric Gondro, Sajid Abdullah, Muhammad Anjum Zia

{"title":"Assessing Genetic Variation and Population Connectivity in Wallago attu: A Call for Enhanced Conservation Measures.","authors":"Taqwa Safdar, Khalid Abbas, Cedric Gondro, Sajid Abdullah, Muhammad Anjum Zia","doi":"10.1007/s10528-025-11156-6","DOIUrl":"https://doi.org/10.1007/s10528-025-11156-6","url":null,"abstract":"Exploited fish species are experiencing population decline and environmental changes due to human activities. As populations decline, genetic diversity may decrease, potentially resulting in a reduced ability to adapt to current and future ecological shifts. The prominent freshwater fish species, Wallago attu, faces extinction threats and has recently been classified as Vulnerable on the IUCN Red List. Little is known about the species' genetic and population dynamics, despite the economic importance in the region. The study aimed to use microsatellites to gather baseline genetic and population data and assess the effect of water management practices on population structure. The study revealed low-to-moderate genetic diversity within sample localities but significantly high differentiation among populations. Findings indicated significant inbreeding within populations and strong evidence of recent bottlenecks. Genetic relatedness analysis, a UPGMA dendrogram and Bayesian clustering, clearly divided the populations into two clusters, which suggests a significant genetic divergence. Anthropogenic activities and water management strategies significantly influenced the genetic population structure in the studied regions. To ensure the long-term survival of this vulnerable species, conservation-oriented management should prioritize preserving current genetic diversity, given its distinct genetic patterns.","PeriodicalId":482,"journal":{"name":"Biochemical Genetics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144273888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HPV16 E7 Enhances Cell Stemness via RTKN2-Mediated Activation of the NF-κB Pathway in Cervical Cancer. hpv16e7通过rtkn2介导的NF-κB通路激活增强宫颈癌细胞干细胞性

IF 2.1 4区生物学

Biochemical Genetics Pub Date : 2025-06-10 DOI: 10.1007/s10528-025-11144-w

Min Yang, Yuejiang Ma, Zhu Cao, Hong Zhan, Ye Jin, Xiufeng Huang, Shizhou Yang

{"title":"HPV16 E7 Enhances Cell Stemness via RTKN2-Mediated Activation of the NF-κB Pathway in Cervical Cancer.","authors":"Min Yang, Yuejiang Ma, Zhu Cao, Hong Zhan, Ye Jin, Xiufeng Huang, Shizhou Yang","doi":"10.1007/s10528-025-11144-w","DOIUrl":"https://doi.org/10.1007/s10528-025-11144-w","url":null,"abstract":"Cervical cancer (CC) is one of the most prevalent cancers among women globally. The primary cause of CC is persistent infection with high-risk types of human papillomavirus (HPV), particularly HPV16, whose E7 oncoprotein plays a pivotal role in carcinogenesis and the maintenance of stem cell-like characteristics. RTKN2 participates in the progression of various cancers. However, the precise functions of RTKN2 in regulating CC remain unclear. The effects of HPV16 E7 in CC cells were evaluated using MTT, western blotting, Transwell, and sphere formation assays. Transcriptome sequencing and bioinformatics analyses were used to identify the targets of HPV16 E7. The expression levels of the target (RTKN2) in clinical samples were assessed using immunohistochemistry (IHC). The function and mechanism of RTKN2 in CC cells were investigated by the knockdown and overexpression approaches, as well as dual-luciferase reporter assay. HPV16 E7 exhibited a positive correlation on the malignant phenotype and stemness of CC cells. RTKN2 was identified as a target of HPV16 E7, and a reduction in its expression levels was caused by knockdown of HPV16 E7. The high expression of RTKN2 was associated with a poor prognosis in CC. HPV16 E7 may regulate RTKN2 expression by modulating the binding activity of E2F1 to the RTKN2 promoter. Upregulated RTKN2 activates the NF-κB signaling pathway, enhances the stemness of CC cells, and ultimately promotes malignant progression.","PeriodicalId":482,"journal":{"name":"Biochemical Genetics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144265005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LDHA-Mediated H3K18 Lactylation Promotes the Glycolysis in Non-Small Cell Lung Cancer Through Targeting PTEN. ldha介导的H3K18乳酸化通过靶向PTEN促进非小细胞肺癌的糖酵解。

IF 2.1 4区生物学

Biochemical Genetics Pub Date : 2025-06-07 DOI: 10.1007/s10528-025-11145-9

Yufeng Shao, Xiaomin Duan, Guojian Gu, Qingfeng Zhu, Jian Shu, Fan Fei

{"title":"LDHA-Mediated H3K18 Lactylation Promotes the Glycolysis in Non-Small Cell Lung Cancer Through Targeting PTEN.","authors":"Yufeng Shao, Xiaomin Duan, Guojian Gu, Qingfeng Zhu, Jian Shu, Fan Fei","doi":"10.1007/s10528-025-11145-9","DOIUrl":"https://doi.org/10.1007/s10528-025-11145-9","url":null,"abstract":"Non-small cell lung cancer (NSCLC) accounts for 85% of all lung cancer cases. Lactylation, a lactate-driven post-translational modification, has been implicated in various tumor pathologies. This study aimed to investigate the role of histone H3 lysine 18 lactylation (H3K18la) in NSCLC progression. Western blot was performed to detect the protein levels of lactylation and H3K18la. Cell counting kit-8 (CCK-8), 5-ethynyl-2'-deoxyuridine (EdU), and Transwell migration assays were performed to detect the cell viability, proliferation, and migration. The 2-deoxy-2-[fluorine-18]fluoro-D-glucose (18F-FDG) uptake rate, lactate content, and extracellular acidification rate (ECAR) were detected by commercial kits. Chromatin immunoprecipitation-qPCR was performed to assess the relative H3K18la enrichment on phosphatase and tensin homolog (PTEN) promoter. Finally, we established the tumor-bearing mouse model. Results showed that A549 and H1299 cells showed increased pan-kla and H3K18la protein levels. Besides, silencing of lactate dehydrogenase A (LDHA) inhibited the cell viability, proliferation, migration, and glycolysis in A549 and H1299 cells. Animal study results indicated that LDHA inhibition suppressed the tumor growth in xenografts mice. Mechanically, LDHA-mediated H3K18la regulated the transcription and stability of PTEN in A549 and H1229 cells. Final rescue results demonstrated that PTEN deficiency increased the cell proliferation, migration, and glycolysis in A549 and H1299 cells. Our study suggested that LDHA-mediated H3K18la promoted the glycolysis in NSCLC through targeting PTEN, which might provide a new insight for NSCLC treatment.","PeriodicalId":482,"journal":{"name":"Biochemical Genetics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144245593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0