Applications in Plant Sciences最新文献

{"title":"Correction to \"The challenges of growing orchids from seeds for conservation: An assessment of asymbiotic techniques\".","authors":"","doi":"10.1002/aps3.70079","DOIUrl":"https://doi.org/10.1002/aps3.70079","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1002/aps3.11496.].</p>","PeriodicalId":8022,"journal":{"name":"Applications in Plant Sciences","volume":" ","pages":"e70079"},"PeriodicalIF":3.5,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13507636/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148824365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A cost-effective DNA extraction protocol for long-read sequencing in non-model plants","authors":"Sofia Gaischuk,&nbsp;Cintia F. Marchetti,&nbsp;Ezequiel Petrillo,&nbsp;Fernando A. Rabanal,&nbsp;Detlef Weigel,&nbsp;Nicolás Bellora,&nbsp;M. Verónica Arana","doi":"10.1002/aps3.70055","DOIUrl":"https://doi.org/10.1002/aps3.70055","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Premise</h3>\u0000 \u0000 <p>Third-generation sequencing has revolutionized genomics, enabling in-depth analysis of genome sequence, structure, and epigenetic features. Yet, extracting high-quality DNA for long-read sequencing remains a bottleneck—particularly in non-model plants, such as mature trees growing in natural environments, which often contain abundant endogenous compounds that hinder extraction and downstream applications.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods and Results</h3>\u0000 \u0000 <p>We developed an optimized, robust, and cost-effective DNA extraction protocol that yields high-quality DNA suitable for Oxford Nanopore Technologies and PacBio sequencing. Validation across diverse taxa—including six <i>Nothofagus</i> species, gymnosperms endemic to Andean–Patagonian forests, exotic conifers of commercial value, and model plants—demonstrated consistently high DNA purity (A₂₆₀/A₂₈₀ &gt; 1.8, A₂₆₀/A₂₃₀ &gt; 2.0) and fragment sizes ≥30 kbp. Downstream sequencing confirmed suitability for applications requiring long, intact molecules and base modification detection.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Compared to commercial kits and standard protocols, this approach achieved superior DNA integrity and yield without specialized equipment, offering an accessible solution for researchers working with challenging plant species.</p>\u0000 </section>\u0000 </div>","PeriodicalId":8022,"journal":{"name":"Applications in Plant Sciences","volume":"14 4","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aps3.70055","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148783732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pearl's causality for integrating ecological datasets: A case study on Myricaria germanica in northern Italy","authors":"Kailin Weitkämper,&nbsp;Kilian Rückschloß,&nbsp;Tommaso Sitzia,&nbsp;Bruno Michielon,&nbsp;Silke Werth,&nbsp;Felix Weitkämper","doi":"10.1002/aps3.70056","DOIUrl":"https://doi.org/10.1002/aps3.70056","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Premise</h3>\u0000 \u0000 <p>Applied ecology can significantly influence policy decisions on environmental issues. Therefore, research in this field should be as transparent and reproducible as possible. Existing expertise from a broad range of disciplines should also be integrated into ecological research to allow researchers to maximize understanding of complex systems.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We illustrate how Pearl's causality can contribute to applied ecology. We demonstrate the implications of causal diagrams for assessing the effects of anthropogenic and abiotic factors in ecological systems, using <i>Myricaria germanica</i> in Italian river systems as an example. In particular, we showcase the interplay between explicit causal modeling and classical statistical techniques.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>In our example, we find that river channel width and riverbank protections are the most important factors affecting the survival of <i>M. germanica</i> juveniles in northern Italy. Other factors such as altitude and other human activity also impact <i>M. germanica</i> survival through channel width as a mediator.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Discussion</h3>\u0000 \u0000 <p>We demonstrate that causal diagrams can be an effective new language for ecological research. The causal diagram highlights that integrating hydrological, historical, or anthropological data could strengthen understanding of <i>M. germanica</i> populations in river systems. This framework facilitates interdisciplinary research and realizes the full potential of ecological datasets.</p>\u0000 </section>\u0000 </div>","PeriodicalId":8022,"journal":{"name":"Applications in Plant Sciences","volume":"14 4","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aps3.70056","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148783736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Techniques and challenges in studying photosynthetic diversity in freshwater submerged vascular plants","authors":"Ellie Loew-Mendelson,&nbsp;David Wickell,&nbsp;Calista Mrozinski,&nbsp;Lizzy Iliana Doan,&nbsp;Jason Hupp,&nbsp;Richard L. Vath,&nbsp;Lingling Yuan,&nbsp;Karolina Heyduk","doi":"10.1002/aps3.70071","DOIUrl":"https://doi.org/10.1002/aps3.70071","url":null,"abstract":"<p>While the photosynthetic diversity of aquatic plants rivals that of terrestrial species, the environmental conditions underlying that diversity fundamentally differ. Despite these environmental differences, aquatic and terrestrial plants have convergently evolved carbon concentrating mechanisms (CCMs). However, characterization of these pathways in submerged plants has lagged behind terrestrial systems due to methodological constraints of the aquatic environment. Here we review and evaluate contemporary methods for detecting CCMs in aquatic plants. Physiological methods, including gas exchange and isotopic analyses, provide valuable insights into terrestrial plants but face significant challenges in aquatic systems. Biochemical assays of organic acid accumulation reliably detect CCMs in aquatic species but may struggle to detect weak or non-canonical CCMs in submerged plants. Gene expression assays of canonical CCM pathway genes may provide a sensitive tool for characterizing photosynthetic diversity in a wide range of aquatic species. While the methods and frameworks developed for terrestrial plants do not necessarily directly translate to aquatic systems, extending these methods and integrating multiple lines of evidence can improve our ability to characterize photosynthetic diversity in aquatic plants.</p>","PeriodicalId":8022,"journal":{"name":"Applications in Plant Sciences","volume":"14 4","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aps3.70071","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148784515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Garryanalyzer: A morphometric workflow and open-source ImageJ plug-in for quantitative morphological analysis of Pacific Northwest Quercus leaves","authors":"Ziqi Xie,&nbsp;Tan Bahar,&nbsp;Keith Karoly","doi":"10.1002/aps3.70069","DOIUrl":"https://doi.org/10.1002/aps3.70069","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Premise</h3>\u0000 \u0000 <p>Accurate species identification is crucial for ecological restoration and can be especially challenging for understudied non-model species. <i>Quercus garryana</i> is the only native oak species in the Pacific Northwest and is an important component of the endangered oak savanna ecosystem. <i>Quercus robur</i> is an imported ornamental species from Europe and has been found to be mistakenly planted as <i>Q. garryana</i> in habitat restoration projects.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We measured leaf morphological traits sampled from herbarium collections in their native ranges using the digital morphometric tools MorphoLeaf and Tomato Analyzer. We then used Lasso logistic analysis to generate a predictive model and tested it on leaves from Portland, Oregon. To streamline this species detection process, we developed Garryanalyzer, an ImageJ plug-in that automatically measures leaf traits and outputs species predictions.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Garryanalyzer demonstrated 95% accuracy in predicting the species identity of herbarium specimens of oaks. Garryanalyzer correctly identified all <i>Q. robur</i> individuals sampled in Portland but showed lower accuracy for <i>Q. garryana</i>.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Discussion</h3>\u0000 \u0000 <p>Many existing morphometric software are not open source, which makes them unable to be customized to specific study systems. Garryanalyzer is built upon the widely used open-source ImageJ platform. This study also demonstrates a viable workflow for developing similar tools for other ecologically important non-model plant species.</p>\u0000 </section>\u0000 </div>","PeriodicalId":8022,"journal":{"name":"Applications in Plant Sciences","volume":"14 4","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aps3.70069","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148785136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-model plants: Challenges and solutions","authors":"José M. Cunill-Flores,&nbsp;Guanjing Hu,&nbsp;Aarón I. Vélez-Ramírez","doi":"10.1002/aps3.70074","DOIUrl":"https://doi.org/10.1002/aps3.70074","url":null,"abstract":"&lt;p&gt;Model organisms have fundamentally advanced plant biology. Research in a limited number of systems has revealed core principles of development, physiology, genetics, ecology, and evolution, and these systems continue to provide a critical foundation for discovery. The plant kingdom, however, is significantly more diverse than the subset of species with established genomic, experimental, and analytical resources. Many traits that require urgent explanation, such as unusual life histories, distinctive metabolic pathways, complex genome architectures, specialized ecological interactions, and responses to rapidly changing environments, are most evident in organisms that remain outside conventional model systems.&lt;/p&gt;&lt;p&gt;The term “non-model” often suggests a lack of reference genomes, standardized protocols, transformation systems, curated databases, or widely adopted analytical pipelines. However, research on non-model plants is not solely defined by these absences. These systems provide opportunities to test the generality of principles inferred from model organisms, to discover biological solutions that have evolved independently or uniquely, and to link methodological innovation to applied needs in conservation, restoration, agriculture, and environmental monitoring.&lt;/p&gt;&lt;p&gt;Research in non-model systems reminds us that methods developed for model organisms cannot always be transferred directly. Protocols that work well in standard model species may fail when researchers are working with difficult tissues, degraded DNA from herbarium specimens, unusual morphologies, aquatic habitats, complex life cycles, polyploid genomes, or groups with very little comparative information. These challenges go beyond technical limitations; they also affect our mindset. If we become too dependent on the categories and assumptions that come from model systems, we risk overlooking important observations or drawing narrow conclusions.&lt;/p&gt;&lt;p&gt;This special issue, “Non-model plants: Challenges and solutions,” addresses the methodological and analytical constraints encountered when studying non-model plants of diverse taxa. The contributions systematically examine the limitations intrinsic to research outside recognized model systems and present solutions that expand the experimental and analytical tools available for the community. Specifically, the studies presented here show that these limitations can be reduced by three principal strategies: first, by adapting experimental and analytical methods when canonical assumptions derived from model organisms do not hold in novel phylogenetic or ecological contexts (Anaya et al., &lt;span&gt;2026&lt;/span&gt;; Garcia et al., &lt;span&gt;2026&lt;/span&gt;; Loew-Mendelson et al., &lt;span&gt;2026&lt;/span&gt;); second, by developing and validating protocols that increase the accessibility and reduce costs of genomic and functional research (Anaya et al., &lt;span&gt;2026&lt;/span&gt;; Bullock et al., &lt;span&gt;2026&lt;/span&gt;; Gaischuk et al., &lt;span&gt;2026&lt;/span&gt;; Garcia et al., &lt;span&gt;20","PeriodicalId":8022,"journal":{"name":"Applications in Plant Sciences","volume":"14 4","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aps3.70074","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148785243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expanded application to plant reproductive tissues of a branched DNA probe-based in situ hybridization method","authors":"Brooklyn M. Anaya,&nbsp;Austin T. Nguyen,&nbsp;Kelly K. S. Matsunaga,&nbsp;Lena C. Hileman","doi":"10.1002/aps3.70020","DOIUrl":"https://doi.org/10.1002/aps3.70020","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Premise</h3>\u0000 \u0000 <p>Detecting clear tissue- and organ-specific patterns of gene expression is key to understanding the genetic mechanisms that control plant development. In situ hybridization (ISH) of mRNA is one of the most precise, yet most challenging approaches to gene expression assays.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods and Results</h3>\u0000 \u0000 <p>Detection of histone H4 expression in reproductive tissues of <i>Mimulus lewisii</i>, a model angiosperm, was optimized using the RNAscope ISH assay. The optimized protocol was used to detect histone H4 expression in reproductive tissues of two gymnosperm species, <i>Taxodium distichum</i> and <i>Juniperus virginiana</i>, without further need for species-specific optimization. Additionally, the optimized protocol was used to detect expression of <i>CYCLOIDEA</i> transcription factors in <i>M. lewisii</i> reproductive tissues without further optimization and with results similar to those previously reported.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The RNAscope assay can quickly and sensitively generate high-quality ISH results in reproductive tissues across a breadth of plant species.</p>\u0000 </section>\u0000 </div>","PeriodicalId":8022,"journal":{"name":"Applications in Plant Sciences","volume":"14 4","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aps3.70020","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148784638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fun-sized library prep: Miniaturization is a valid method for per-sample cost reduction in targeted sequencing of angiosperm DNA","authors":"Madison R. Bullock,&nbsp;Mohamed Fokar,&nbsp;Rylee D. Creek,&nbsp;Eliot A. Stevens,&nbsp;Matthew G. Johnson","doi":"10.1002/aps3.70070","DOIUrl":"https://doi.org/10.1002/aps3.70070","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Premise</h3>\u0000 \u0000 <p>Genomic analysis of population structure is important for understanding plant genetic diversity among and within populations. A limitation to the broader application of genetics in conservation and related fields is the cost of obtaining large datasets. Targeted sequencing and low-volume robotic liquid handlers can reduce library preparation reaction volumes and costs.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We used targeted sequencing via Angiosperms353 to obtain data for 768 samples, 18 of which were identical, at 0.5× and 0.1× reaction volumes. We calculated quality and quantity control statistics to compare the effects of tissue age and library reaction volume on sequencing results for on-target nuclear and off-target plastid genes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Library miniaturization to 0.1× reduces costs and generally performs comparably to 0.5× libraries. In the full dataset, Tenth-Volume Only libraries showed smaller insert sizes and fewer genes with mapped sequences, but no reduction in mapped reads. In the Overlap Set, 0.1× libraries had equal or improved performance with no significant decrease in sequencing efficiency. Differences by tissue type likely reflected sampling variation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Discussion</h3>\u0000 \u0000 <p>Miniaturization to 0.1× substantially reduces per-sample costs while maintaining comparable sequencing quality across fresh and herbarium angiosperm DNA. Overall, miniaturization provides a reliable, cost-effective approach for targeted sequencing, increasing the feasibility of using herbarium collections and enabling broader access to population-level genomic studies.</p>\u0000 </section>\u0000 </div>","PeriodicalId":8022,"journal":{"name":"Applications in Plant Sciences","volume":"14 4","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aps3.70070","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148785135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enabling the study of gene function in gymnosperms: Virus-induced gene silencing in Ephedra tweedieana","authors":"Anthony G. K. Garcia,&nbsp;Jo Trang Bùi,&nbsp;Todd P. Michael,&nbsp;Stefanie M. Ickert-Bond,&nbsp;Verónica S. Di Stilio","doi":"10.1002/aps3.70027","DOIUrl":"https://doi.org/10.1002/aps3.70027","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Premise</h3>\u0000 \u0000 <p>As the sister clade to angiosperms, extant gymnosperms are crucial for reconstructing ancestral gene regulatory networks in seed plants. This highlights the need for model systems representing each of their distinct lineages. However, tools to quickly and effectively investigate gene function in gymnosperms are still limited due to the challenges of long life cycles and large genome sizes. Species within the xerophytic genus <i>Ephedra</i> (Gnetales) have comparatively smaller genomes and shrubby growth habits with shorter life spans, making them better suited for greenhouse cultivation and laboratory experiments.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We implement virus-induced gene silencing (VIGS) to manipulate gene expression in <i>Ephedra tweedieana</i> via <i>Agrobacterium</i>-mediated vacuum infiltration of tobacco rattle virus (TRV1 and TRV2) into seedlings.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Treatment resulted in highly efficient gene silencing of the <i>E. tweedieana PHYTOENE DESATURASE</i> (<i>PDS</i>) ortholog <i>EtwPDS</i>. The expected photobleaching phenotype was observed as early as two weeks, and lasted at least five months in stems, shoot tips, leaves, axillary meristems, and lateral branches of treated plants.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Discussion</h3>\u0000 \u0000 <p>We report on virus-induced targeted gene silencing of <i>PDS</i> in a Gnetales representative to further enable functional studies of the genetic mechanisms underpinning adaptations in gymnosperms, an important and underrepresented lineage of seed plants.</p>\u0000 </section>\u0000 </div>","PeriodicalId":8022,"journal":{"name":"Applications in Plant Sciences","volume":"14 4","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aps3.70027","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148783692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BarnebyLives: An R package to create herbarium specimen labels and clean spreadsheets.","authors":"Reed Clark Benkendorf, Jeremie B Fant","doi":"10.1002/aps3.70077","DOIUrl":"https://doi.org/10.1002/aps3.70077","url":null,"abstract":"<p><strong>Premise: </strong>Accessioning herbarium specimens is labor intensive, yet remains vital for research in ecology, evolution, and conservation. As institutional support for herbaria declines, efficient tools are needed to streamline this process. The R package BarnebyLives was developed to assist collectors by supplementing collection notes, verifying taxonomic data, conducting quality checks, generating labels, and submitting digital records.</p><p><strong>Methods and results: </strong>BarnebyLives integrates geospatial data from U.S. government sources to provide jurisdictional and site information and checks taxonomic names using in-house spell checkers, International Plant Names Index (IPNI) author standards, and Kew's Plants of the World Online. Optional features include generating Google Maps driving directions. The tool outputs data in tabular and spatial formats for review before producing LaTeX-based labels and shipping manifests.</p><p><strong>Conclusions: </strong>BarnebyLives improves data accuracy, ensures up-to-date taxonomy, and significantly reduces the time and effort required to accession herbarium specimens in the United States.</p>","PeriodicalId":8022,"journal":{"name":"Applications in Plant Sciences","volume":" ","pages":"e70077"},"PeriodicalIF":3.5,"publicationDate":"2026-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13490981/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148787411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}