A comprehensive illustrated protocol for clearing, mounting, and imaging leaf venation networks

IF 2.7 3区生物学 Q2 PLANT SCIENCES

Applications in Plant Sciences Pub Date : 2025-03-07 DOI:10.1002/aps3.70002

Isabella Niewiadomski, Monica Antonio, Luiza Maria T. Aparecido, Mickey Boakye, Sonoma Carlos, Andrea Echevarria, Adrian Fontao, Joseph Mann, Ilaíne Silveira Matos, Norma Salinas, Bradley Vu, Benjamin Wong Blonder

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引用次数: 0

Abstract

Premise

Leaf venation network architecture can provide insights into plant evolution, ecology, and physiology. Venation networks are typically assessed through histological methods, but existing protocols provide limited guidance on processing large or challenging leaves.

Methods and results

We present an illustrated protocol for visualizing whole leaf venation networks, including sample preparation, clearing, staining, mounting, imaging, and archiving steps. The protocol also includes supply lists, troubleshooting procedures, safety considerations, and examples of successful and unsuccessful outcomes. The protocol is suitable for a wide range of leaf sizes and morphologies and has been used with all major plant groups.

Conclusion

We provide a workflow for obtaining high-quality mounts and images of venation networks of a wide range of species, using readily available materials.

Abstract Image

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一个全面的说明协议清理，安装，和成像叶脉网络

前提是叶脉网络结构可以提供对植物进化、生态学和生理学的见解。脉网络通常通过组织学方法进行评估，但现有的协议在处理大叶片或具有挑战性的叶片方面提供有限的指导。方法和结果我们提出了一个可视化全叶脉网络的说明方案，包括样品制备，清除，染色，安装，成像和存档步骤。该协议还包括供应清单、故障排除程序、安全考虑以及成功和不成功结果的示例。该协议适用于广泛的叶片大小和形态，并已用于所有主要植物群。结论本研究提供了一种工作流程，可以利用现成的材料获得多种物种的高质量血管网络图和图像。

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来源期刊

Applications in Plant Sciences PLANT SCIENCES-

CiteScore

7.30

自引率

0.00%

发文量

审稿时长

12 weeks

期刊介绍： Applications in Plant Sciences (APPS) is a monthly, peer-reviewed, open access journal promoting the rapid dissemination of newly developed, innovative tools and protocols in all areas of the plant sciences, including genetics, structure, function, development, evolution, systematics, and ecology. Given the rapid progress today in technology and its application in the plant sciences, the goal of APPS is to foster communication within the plant science community to advance scientific research. APPS is a publication of the Botanical Society of America, originating in 2009 as the American Journal of Botany''s online-only section, AJB Primer Notes & Protocols in the Plant Sciences. APPS publishes the following types of articles: (1) Protocol Notes describe new methods and technological advancements; (2) Genomic Resources Articles characterize the development and demonstrate the usefulness of newly developed genomic resources, including transcriptomes; (3) Software Notes detail new software applications; (4) Application Articles illustrate the application of a new protocol, method, or software application within the context of a larger study; (5) Review Articles evaluate available techniques, methods, or protocols; (6) Primer Notes report novel genetic markers with evidence of wide applicability.