Georgina González-Rebeles, Miguel Ángel Alonso-Arevalo, Eulogio López, Rodrigo Méndez-Alonzo
{"title":"一种低成本的脆弱性曲线光学法计算P50的方案","authors":"Georgina González-Rebeles, Miguel Ángel Alonso-Arevalo, Eulogio López, Rodrigo Méndez-Alonzo","doi":"10.1002/aps3.70004","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Premise</h3>\n \n <p>The quantification of plant drought resistance, particularly embolism formation, within and across species, is critical for ecosystem management and agriculture. We developed a cost-effective protocol to measure the water potential at which 50% of hydraulic conductivity (<i>P</i><sub>50</sub>) is lost in stems, using affordable and accessible materials in comparison to the traditional optical method.</p>\n </section>\n \n <section>\n \n <h3> Methods and Results</h3>\n \n <p>Our protocol uses inexpensive USB microscopes, which are secured along with the plants to a pegboard base to avoid movement. A Python program automatized the image acquisition. This method was applied to quantify <i>P</i><sub>50</sub> in an exotic species (<i>Nicotiana glauca</i>) and native species (<i>Rhus integrifolia</i>) of the Mediterranean vegetation in Baja California, Mexico.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>The intra- and interspecific patterns of variation in stem <i>P</i><sub>50</sub> of <i>N. glauca</i> and <i>R. integrifolia</i> were obtained using the low-cost optical method with widely available and affordable materials that can be easily replicated for other species.</p>\n </section>\n </div>","PeriodicalId":8022,"journal":{"name":"Applications in Plant Sciences","volume":"13 2","pages":""},"PeriodicalIF":2.7000,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aps3.70004","citationCount":"0","resultStr":"{\"title\":\"A low-cost protocol for the optical method of vulnerability curves to calculate P50\",\"authors\":\"Georgina González-Rebeles, Miguel Ángel Alonso-Arevalo, Eulogio López, Rodrigo Méndez-Alonzo\",\"doi\":\"10.1002/aps3.70004\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Premise</h3>\\n \\n <p>The quantification of plant drought resistance, particularly embolism formation, within and across species, is critical for ecosystem management and agriculture. We developed a cost-effective protocol to measure the water potential at which 50% of hydraulic conductivity (<i>P</i><sub>50</sub>) is lost in stems, using affordable and accessible materials in comparison to the traditional optical method.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods and Results</h3>\\n \\n <p>Our protocol uses inexpensive USB microscopes, which are secured along with the plants to a pegboard base to avoid movement. A Python program automatized the image acquisition. This method was applied to quantify <i>P</i><sub>50</sub> in an exotic species (<i>Nicotiana glauca</i>) and native species (<i>Rhus integrifolia</i>) of the Mediterranean vegetation in Baja California, Mexico.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusions</h3>\\n \\n <p>The intra- and interspecific patterns of variation in stem <i>P</i><sub>50</sub> of <i>N. glauca</i> and <i>R. integrifolia</i> were obtained using the low-cost optical method with widely available and affordable materials that can be easily replicated for other species.</p>\\n </section>\\n </div>\",\"PeriodicalId\":8022,\"journal\":{\"name\":\"Applications in Plant Sciences\",\"volume\":\"13 2\",\"pages\":\"\"},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2025-03-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aps3.70004\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Applications in Plant Sciences\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/aps3.70004\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applications in Plant Sciences","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/aps3.70004","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
A low-cost protocol for the optical method of vulnerability curves to calculate P50
Premise
The quantification of plant drought resistance, particularly embolism formation, within and across species, is critical for ecosystem management and agriculture. We developed a cost-effective protocol to measure the water potential at which 50% of hydraulic conductivity (P50) is lost in stems, using affordable and accessible materials in comparison to the traditional optical method.
Methods and Results
Our protocol uses inexpensive USB microscopes, which are secured along with the plants to a pegboard base to avoid movement. A Python program automatized the image acquisition. This method was applied to quantify P50 in an exotic species (Nicotiana glauca) and native species (Rhus integrifolia) of the Mediterranean vegetation in Baja California, Mexico.
Conclusions
The intra- and interspecific patterns of variation in stem P50 of N. glauca and R. integrifolia were obtained using the low-cost optical method with widely available and affordable materials that can be easily replicated for other species.
期刊介绍：
Applications in Plant Sciences (APPS) is a monthly, peer-reviewed, open access journal promoting the rapid dissemination of newly developed, innovative tools and protocols in all areas of the plant sciences, including genetics, structure, function, development, evolution, systematics, and ecology. Given the rapid progress today in technology and its application in the plant sciences, the goal of APPS is to foster communication within the plant science community to advance scientific research. APPS is a publication of the Botanical Society of America, originating in 2009 as the American Journal of Botany''s online-only section, AJB Primer Notes & Protocols in the Plant Sciences.
APPS publishes the following types of articles: (1) Protocol Notes describe new methods and technological advancements; (2) Genomic Resources Articles characterize the development and demonstrate the usefulness of newly developed genomic resources, including transcriptomes; (3) Software Notes detail new software applications; (4) Application Articles illustrate the application of a new protocol, method, or software application within the context of a larger study; (5) Review Articles evaluate available techniques, methods, or protocols; (6) Primer Notes report novel genetic markers with evidence of wide applicability.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
