Acta Histochemica Et Cytochemica最新文献

Carbon Black Deposition in Regional Lymph Nodes Is Associated with Disease Control after Immune Checkpoint Inhibitor-Based Therapy in Recurrent Esophageal Squamous Cell Carcinoma. 基于免疫检查点抑制剂治疗复发性食管鳞状细胞癌后，区域淋巴结炭黑沉积与疾病控制相关

IF 1.9 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2026-08-29 Epub Date: 2026-08-22 DOI: 10.1267/ahc.26-00018

Teruki Sakoh, Kosuke Kanemitsu, Yoshihiro Komohara, Daiki Yoshii, Cheng Pan, Rin Yamada, Yukio Fujiwara, Yoshiyuki Tagayasu, Kohei Yamashita, Keisuke Kosumi, Kazuto Harada, Yoshifumi Baba, Masaaki Iwatsuki

{"title":"Carbon Black Deposition in Regional Lymph Nodes Is Associated with Disease Control after Immune Checkpoint Inhibitor-Based Therapy in Recurrent Esophageal Squamous Cell Carcinoma.","authors":"Teruki Sakoh, Kosuke Kanemitsu, Yoshihiro Komohara, Daiki Yoshii, Cheng Pan, Rin Yamada, Yukio Fujiwara, Yoshiyuki Tagayasu, Kohei Yamashita, Keisuke Kosumi, Kazuto Harada, Yoshifumi Baba, Masaaki Iwatsuki","doi":"10.1267/ahc.26-00018","DOIUrl":"10.1267/ahc.26-00018","url":null,"abstract":"Carbon black (CB) deposition, or anthracosis, is frequently observed in the regional lymph nodes (RLNs) of patients with thoracic malignancies, particularly those with a history of smoking; however, its clinical significance in immune checkpoint inhibitor (ICI)-based therapy remains unclear. This retrospective study aimed to investigate the association between CB deposition in RLNs and response to ICI-based therapy in recurrent esophageal squamous cell carcinoma (ESCC). The study included 47 patients with recurrent ESCC who underwent curative-intent esophagectomy and subsequently received ICI-based therapy. The proportion of CB-containing RLNs (%CB) was histologically evaluated, and patients were divided into high- and low-CB groups according to the median %CB value. CB was mainly detected in lymph node sinus macrophages. Patients in the high-CB group showed a significantly higher disease control rate than those in the low-CB group. Progression-free survival tended to be longer in the high-CB group, although the difference did not reach statistical significance. CB-containing sinus macrophages expressed CD68 and CD163, whereas CD169 expression was markedly reduced or absent. No significant association was observed between %CB and tumor-infiltrating immune cell densities or HLA class I/PD-L1 expression. By contrast, ectopic HLA-DR expression in cancer cells was more frequent in the high-CB group. These findings suggest that a higher proportion of CB-containing RLNs is associated with disease control following ICI-based therapy in recurrent ESCC. Histological assessment of CB deposition in RLNs may provide useful information regarding clinical benefit from ICI-based immunotherapy.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"59 4","pages":"135-141"},"PeriodicalIF":1.9,"publicationDate":"2026-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13554460/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148917802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Simple Mammalian Whole Embryo Culture System to Study High-Temperature Effects on Development. 研究高温对发育影响的简单哺乳动物全胚培养系统。

IF 1.9 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2026-08-29 Epub Date: 2026-07-25 DOI: 10.1267/ahc.26-00006

Tatsuhiro Nagata, Daigo Saito, Yumi Yoshida, Sayaka Tojima, Tadashi Nomura

{"title":"A Simple Mammalian Whole Embryo Culture System to Study High-Temperature Effects on Development.","authors":"Tatsuhiro Nagata, Daigo Saito, Yumi Yoshida, Sayaka Tojima, Tadashi Nomura","doi":"10.1267/ahc.26-00006","DOIUrl":"10.1267/ahc.26-00006","url":null,"abstract":"Whole embryo culture is a unique system for developing rodent embryos outside the uterus. This system enables the observation and manipulation of mammalian embryos during specific periods of embryonic development. Established techniques utilize a rotating bottle system that continuously supplies oxygen. However, commercial devices are currently unavailable, making the new implementation of this system virtually impossible. Furthermore, the conventional device had limitations in temperature settings, making embryonic development studies impossible under conditions deviating from the optimal temperature for mammalian embryos. In this study, we developed a simple rotating culture system that can be installed in a commercially available incubator. Embryo growth and physiological status in this devise are equivalent to those in embryos cultured in the conventional system. Using this system, we clarified the harmful effects of hyperthermia during the early stages of mouse embryonic development. This device is fully suitable for whole embryo culture in mammals and provides a foundation for verifying embryonic development states in rodents under various temperature conditions.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"59 4","pages":"151-158"},"PeriodicalIF":1.9,"publicationDate":"2026-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13554458/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148917723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterogeneous Expression of TREM2 And STAB1 Among Tumor-Associated Macrophages in Clear-Cell Renal Cell Carcinoma. 透明细胞肾细胞癌肿瘤相关巨噬细胞中TREM2和STAB1的异质表达

IF 1.9 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2026-08-29 DOI: 10.1267/ahc.26-00027

Ren Shirotani, Tomoka Takahashi, Jie Su, Ayano Uekawa, Yojiro Ozaki, Yuki Ibe, Cheng Pan, Daiki Yoshii, Yukio Fujiwara, Toshiki Anami, Hidekazu Nishizawa, Ryoma Kurahashi, Yoji Murakami, Junji Yatsuda, Tomomi Kamba, Yoshihiro Komohara

{"title":"Heterogeneous Expression of TREM2 And STAB1 Among Tumor-Associated Macrophages in Clear-Cell Renal Cell Carcinoma.","authors":"Ren Shirotani, Tomoka Takahashi, Jie Su, Ayano Uekawa, Yojiro Ozaki, Yuki Ibe, Cheng Pan, Daiki Yoshii, Yukio Fujiwara, Toshiki Anami, Hidekazu Nishizawa, Ryoma Kurahashi, Yoji Murakami, Junji Yatsuda, Tomomi Kamba, Yoshihiro Komohara","doi":"10.1267/ahc.26-00027","DOIUrl":"10.1267/ahc.26-00027","url":null,"abstract":"Tumor-associated macrophages (TAMs) play important roles in shaping the tumor microenvironment and regulating antitumor immune responses. Triggering receptor expressed on myeloid cells 2 (TREM2) and stabilin-1 (STAB1/CLEVER1) have recently attracted attention as molecules associated with immunosuppressive macrophage phenotypes; however, their expression patterns in clear-cell renal cell carcinoma (ccRCC) remain unclear. This study aimed to examine TREM2 and STAB1 expression in ccRCC using public transcriptomic datasets and public single-cell RNA sequencing data, as well as immunohistochemistry using ccRCC specimens. In the TCGA cohort, high TREM2 and STAB1 mRNA expression was associated with poor overall survival and advanced tumor stage. In an independent immunohistochemistry cohort, TREM2 was expressed mainly in TAMs, whereas STAB1 was expressed in both TAMs and endothelial cells. TREM2-positive TAM density was significantly associated with high nuclear grade, whereas STAB1-positive TAM density was not. Double immunohistochemistry and single-cell RNA sequencing analyses suggested different, but partially overlapping, expression patterns of TREM2 and STAB1 among TAMs. TREM2-high TAMs showed features of monocyte-derived macrophages, whereas STAB1-high TAMs showed features associated with tissue-resident macrophages. These findings suggest that TREM2-positive TAMs and STAB1-positive TAMs contribute differently to the immune microenvironment of ccRCC. Further studies are needed to clarify their functional significance and therapeutic potential.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"59 4","pages":"143-150"},"PeriodicalIF":1.9,"publicationDate":"2026-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13554459/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148917804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential Expression Patterns of Astrocyte Markers in The Adult Mouse Brain. 星形胶质细胞标记物在成年小鼠脑中的差异表达模式。

IF 1.9 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2026-06-27 Epub Date: 2026-06-24 DOI: 10.1267/ahc.26-00008

Yukino Kawaguchi, Yuri Terashima, Satoshi Tanaka, Hiroaki Okuda

{"title":"Differential Expression Patterns of Astrocyte Markers in The Adult Mouse Brain.","authors":"Yukino Kawaguchi, Yuri Terashima, Satoshi Tanaka, Hiroaki Okuda","doi":"10.1267/ahc.26-00008","DOIUrl":"10.1267/ahc.26-00008","url":null,"abstract":"Astrocytes are the most common glial cells in the mammalian central nervous system. Their diverse structures and functions, along with their critical role as key regulators of brain activity, have recently gained recognition. However, the lack of specific cell markers makes it difficult to identify particular subpopulations. In this study, we examined the distribution of traditional astrocyte markers-glial fibrillary acidic protein (GFAP), SRY-box9 (SOX9), and S100 protein beta (S100β)-in the adult mouse brain. In the cerebral cortex, striatum, and thalamus, most cells did not express GFAP, with limited overlap between GFAP and either SOX9 or S100β. In contrast, the pons-medulla showed a higher rate of co-expression of GFAP and SOX9 compared to the cerebral cortex. In the hippocampus, GFAP was widely expressed and showed significant overlap with SOX9 and S100β. Co-staining of SOX9 and S100β was frequently observed in the cerebral cortex, striatum, thalamus, and midbrain, and S100β-positive cells were more common than SOX9-positive cells. These results reveal regional differences in astrocyte marker expression, highlight the limitations of relying on a single marker like GFAP, and underscore the importance of using multiple markers.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"59 3","pages":"123-128"},"PeriodicalIF":1.9,"publicationDate":"2026-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13319657/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148366495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nuclear Speckle Protein SRRM2 Co-localized with Pathological Tau (pTauS396) in Neuronal and Glial Cells in Progressive Supranuclear Palsy. 核斑蛋白SRRM2与病理性Tau蛋白（pTauS396）在进行性核上性麻痹的神经元和胶质细胞中共定位。

IF 1.9 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2026-06-27 Epub Date: 2026-06-17 DOI: 10.1267/ahc.26-00005

Faris Hazwan Nazar, Aslina Pahrudin Arrozi, Tomoko Kato, Daijiro Yanagisawa, Yasushi Itoh, Ikuo Tooyama, Jean-Pierre Bellier

{"title":"Nuclear Speckle Protein SRRM2 Co-localized with Pathological Tau (pTauS396) in Neuronal and Glial Cells in Progressive Supranuclear Palsy.","authors":"Faris Hazwan Nazar, Aslina Pahrudin Arrozi, Tomoko Kato, Daijiro Yanagisawa, Yasushi Itoh, Ikuo Tooyama, Jean-Pierre Bellier","doi":"10.1267/ahc.26-00005","DOIUrl":"10.1267/ahc.26-00005","url":null,"abstract":"Progressive Supranuclear Palsy (PSP) is a primary 4-repeat tauopathy characterized by progressive motor and cognitive decline. Like other tauopathies, tau misfolding and aggregation are prominent but yield PSP-specific features such as tufted astrocytes and globose neurofibrillary tangles. Emerging evidence suggests that nuclear speckle disassembly and mislocalization of RNA-binding proteins, including serine/arginine repetitive matrix protein 2 (SRRM2), may contribute to disease progression, though SRRM2's role in PSP remains unclear. To assess its association with tau pathology, we examined SRRM2 distribution in midbrain neurons, as well as in astrocytes from both cortical and midbrain regions, using post-mortem immunohistochemistry, immunofluorescence, and 3D reconstruction. In PSP midbrain, neuronal SRRM2 immunoreactivity was markedly elevated compared to controls and co-localized with pTauS396, with >80% overlap; co-localization strongly correlated with SRRM2 abundance (r = 0.9809, p = 0.0191). 3D analysis revealed heterogeneity across cases (PSP-1 to PSP-4) in aggregate morphology, SRRM2 levels, and tau associations. In tufted astrocytes, pTauS396 signals were detected in PSP-1 cortex and PSP-4 midbrain. SRRM2 was absent or faint, yet 3D imaging revealed near-complete SRRM2-pTauS396 co-localization (99% in PSP-1 cortex, 89% in PSP-4 midbrain), regardless of SRRM2 abundance. These findings highlight SRRM2 association with pTauS396 in tangle of PSP.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"59 3","pages":"111-121"},"PeriodicalIF":1.9,"publicationDate":"2026-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13319659/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148366499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Subset of Folliculo-Stellate Cells Expresses Gpr37l1 in The Adult Rat Anterior Pituitary. 成年大鼠垂体前叶卵泡星状细胞亚群表达Gpr37l1。

IF 1.9 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2026-06-27 Epub Date: 2026-05-30 DOI: 10.1267/ahc.26-00012

Taichi Suzuki, Kotaro Horiguchi, Yoko Fujiwara, Ken Fujiwara

{"title":"A Subset of Folliculo-Stellate Cells Expresses Gpr37l1 in The Adult Rat Anterior Pituitary.","authors":"Taichi Suzuki, Kotaro Horiguchi, Yoko Fujiwara, Ken Fujiwara","doi":"10.1267/ahc.26-00012","DOIUrl":"10.1267/ahc.26-00012","url":null,"abstract":"G protein-coupled receptor 37 (GPR37) and GPR37-like 1 (GPR37L1) are orphan G protein-coupled receptors enriched in the central nervous system. However, their cellular distribution in pituitary tissue remains unclear. Here, we mapped Gpr37l1 expression in the adult rat anterior pituitary and identified the expressing cell type(s). Quantitative real-time PCR demonstrated that Gpr37l1 mRNA is significantly more abundant than Gpr37 in the anterior lobe. In situ hybridization (ISH) revealed sparsely distributed Gpr37l1-positive cells throughout the anterior lobe, and the signals did not colocalize with major hormone-producing cell types. Combined ISH and immunohistochemistry further showed that Gpr37l1 mRNA is localized to S100B protein-immunoreactive cells, commonly referred to as folliculo-stellate (FS) cells, and that only a subset of S100B-positive FS cells expresses Gpr37l1. Single-cell RNA-seq analysis corroborated these findings, as Gpr37l1-expressing cells were distributed within the broader S100b-expressing cluster, without forming a distinct subcluster, while showing no detectable Gpr37l1 expression in endocrine lineages or vascular-associated cell populations. Together, these data provide a cell-type-resolved map of Gpr37l1 expression in the adult rat anterior pituitary and identify Gpr37l1 as a marker of heterogeneity within FS cells, establishing a foundation for future studies on GPR37L1 signaling in pituitary microenvironmental regulation.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"59 3","pages":"129-134"},"PeriodicalIF":1.9,"publicationDate":"2026-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13319658/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148366549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IF 1.9 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2026-04-25 Epub Date: 2026-04-16 DOI: 10.1267/ahc.25-00051

Kosuke Kanemitsu, Rin Yamada, Daiki Yoshii, Yukio Fujiwara, Yoshiyuki Tagayasu, Teruki Sako, Kota Arima, Kohei Yamashita, Kazuto Harada, Norihisa Hanada, Masaaki Iwatsuki, Yoshihiro Komohara

{"title":"Aging-Related Morphological Change in Regional Lymph Nodes Promote an Immunosuppressive Microenvironment in Colorectal Cancer.","authors":"Kosuke Kanemitsu, Rin Yamada, Daiki Yoshii, Yukio Fujiwara, Yoshiyuki Tagayasu, Teruki Sako, Kota Arima, Kohei Yamashita, Kazuto Harada, Norihisa Hanada, Masaaki Iwatsuki, Yoshihiro Komohara","doi":"10.1267/ahc.25-00051","DOIUrl":"10.1267/ahc.25-00051","url":null,"abstract":"Lymph nodes (LNs) are critical components of anti-cancer immune responses, orchestrating antigen presentation to T-cells. Aging impairs immune function, but its impact on regional LNs in cancer remains poorly understood. This study investigated age-related morphological and immunological changes in mesenteric LNs and their association with the immune microenvironment in colorectal cancer (CRC). A total of 160 patients who underwent curative resection for CRC were retrospectively analyzed. LN ectasia, defined as sinusoidal dilation accompanied by a reduction in LN sinus macrophages, was evaluated histologically. LN ectasia was significantly more frequent in elderly patients and in right-sided colon cancers. Patients with pronounced LN ectasia showed reduced serum albumin and leukocyte counts. Immunohistochemical analysis revealed that marked LN ectasia was associated with decreased infiltration of CD8+ T cells and Iba1+/CD163+ tumor-associated macrophages in the primary cancer lesion, consistent with a \"cold\" immune microenvironment. Although LN ectasia was not directly associated with prognosis, it reflected systemic immunosenescence and attenuated local immune activation. These findings suggest that LN ectasia serves as a histological marker of aging-related LN dysfunction, contributing to diminished anti-cancer immunity in CRC.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"59 2","pages":"69-74"},"PeriodicalIF":1.9,"publicationDate":"2026-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13122372/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147759268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Crucial Role of GPR30 and Histone Crotonylation during the Inflammatory Repair Process in Ligature-Induced Periodontitis. GPR30和组蛋白Crotonylation在结扎诱导的牙周炎炎症修复过程中的关键作用。

IF 1.9 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2026-04-25 Epub Date: 2026-04-22 DOI: 10.1267/ahc.26-00002

Fidya, Takumi Ishizuka, Toshiki Kubota, Kengo Kai, Yoshitaka Hishikawa

{"title":"The Crucial Role of GPR30 and Histone Crotonylation during the Inflammatory Repair Process in Ligature-Induced Periodontitis.","authors":"Fidya, Takumi Ishizuka, Toshiki Kubota, Kengo Kai, Yoshitaka Hishikawa","doi":"10.1267/ahc.26-00002","DOIUrl":"10.1267/ahc.26-00002","url":null,"abstract":"Periodontitis is a chronic inflammatory disease characterized by collagen degradation and alveolar bone loss. Although estrogen contributes to periodontal homeostasis, the role of the membrane-bound G protein-coupled estrogen receptor 30 (GPR30) in periodontitis remains unclear. This study investigated the temporal involvement of GPR30 in ligature-induced periodontitis, focusing on alveolar bone changes, collagen integrity, fibroblast activation, and epigenetic regulation. Twenty male Wistar rats were allocated to control and periodontitis groups and evaluated at days 7, 14, and 21 following placements of a stainless-steel ligature around the maxillary first molars. Alveolar bone loss was assessed radiographically, while inflammatory changes and collagen organization were examined using hematoxylin-eosin and Masson's trichrome staining. Immunohistochemistry was performed to evaluate GPR30, collagen I/III, α-smooth muscle actin (α-SMA), and histone modifications. Statistical analysis was conducted using one-way ANOVA with Tukey's post hoc test. Alveolar bone loss and collagen degradation were detectable on day 7 and peaked at day 14. GPR30 expression increased during the active inflammatory phase, accompanied by elevated α-SMA and histone H4 lysine 8 crotonylation (H4K8cr), and declined by day 21. Fibroblast transiently adopted a myofibroblast phenotype during inflammation and regressed during early tissue repair. Temporal changes in H4K8cr closely paralleled GPR30 expression. These findings indicate that GPR30 is dynamically associated with inflammatory and remodeling phases of periodontitis and may contribute to epigenetic regulation during periodontal tissue remodeling.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"59 2","pages":"89-99"},"PeriodicalIF":1.9,"publicationDate":"2026-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13122371/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147759346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Semantic Segmentation for Pixel-Wise Visualization of Breast Cancer in Deep Ultraviolet-Excited Fluorescence Images. 基于语义分割的乳腺癌深紫外激发荧光图像逐像素可视化。

IF 1.9 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2026-04-25 Epub Date: 2026-04-22 DOI: 10.1267/ahc.26-00007

Tomoya Matsui, Ryuta Nakao, Shunsuke Tomimoto, Junya Sato, Hirohiko Niioka, Ippei Takada, Yoshinori Harada, Hideo Tanaka, Eiichi Konishi, Hajime Nagahara, Midori Morita, Yasuto Naoi, Tetsuya Taguchi, Tetsuro Takamatsu

{"title":"Semantic Segmentation for Pixel-Wise Visualization of Breast Cancer in Deep Ultraviolet-Excited Fluorescence Images.","authors":"Tomoya Matsui, Ryuta Nakao, Shunsuke Tomimoto, Junya Sato, Hirohiko Niioka, Ippei Takada, Yoshinori Harada, Hideo Tanaka, Eiichi Konishi, Hajime Nagahara, Midori Morita, Yasuto Naoi, Tetsuya Taguchi, Tetsuro Takamatsu","doi":"10.1267/ahc.26-00007","DOIUrl":"10.1267/ahc.26-00007","url":null,"abstract":"Microscopy with ultraviolet surface excitation (MUSE) enables rapid fluorescence imaging of tissue surfaces, but MUSE images differ markedly from conventional hematoxylin and eosin images, making cancer delineation challenging for routine pathological practice. We investigated the feasibility of deep learning-based semantic segmentation, which assigns a class label to each pixel, for pixel-wise breast cancer detection in MUSE images. Fresh breast tissues from 30 mastectomy patients with breast cancer were stained with terbium and Hoechst and imaged by MUSE. A total of 150 cancerous images (five per case) were manually annotated into cancerous and non-cancerous classes, and 300 non-cancerous images (ten per case) were collected. Models were trained and evaluated using five-fold nested cross-validation, comparing a cancer-only (CO) model trained solely on cancerous images with a cancer plus non-cancer (CN) model trained on both cancerous and non-cancerous images. The CO model achieved a higher Dice score than the CN model (CO, 0.7478; CN, 0.7343). Sliding window-based majority voting post-processing reduced scattered false-positive areas and improved Dice scores (CO, 0.7984; CN, 0.7849). These results support the feasibility of deep learning-based semantic segmentation for visualizing breast cancer regions and provide a basis for future quantitative applications using MUSE images.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"59 2","pages":"101-109"},"PeriodicalIF":1.9,"publicationDate":"2026-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13122366/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147759297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of In Vivo Cryotechnique for Morphological Visualization of Nerve and Vascular Structures in a Sciatic Nerve Crush Model. 活体冷冻技术在坐骨神经挤压模型中神经和血管结构形态可视化的应用。

IF 1.9 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2026-04-25 Epub Date: 2026-04-08 DOI: 10.1267/ahc.25-00048

Kyosuke Fukuda, Yuta Sakamoto, Satoshi Shimo, Takashi Amari, Chiharu Takasu, Keisuke Kubota, Kenji Murata, Naohiko Kanemura

{"title":"Application of In Vivo Cryotechnique for Morphological Visualization of Nerve and Vascular Structures in a Sciatic Nerve Crush Model.","authors":"Kyosuke Fukuda, Yuta Sakamoto, Satoshi Shimo, Takashi Amari, Chiharu Takasu, Keisuke Kubota, Kenji Murata, Naohiko Kanemura","doi":"10.1267/ahc.25-00048","DOIUrl":"10.1267/ahc.25-00048","url":null,"abstract":"After a crush injury in sciatic nerve fibers, dynamic changes in blood circulation and immune-cell mobilization occur during axonal regeneration. High-resolution visualization under near-physiological conditions is crucial for understanding these mechanisms. Conventional histological techniques introduce perfusion- and dehydration-induced artifacts that obscure circulation. We employed the in vivo cryotechnique (IVCT) to visualize blood flow within sciatic nerve fibers and assess temporal changes during regeneration. In uninjured mice, IVCT preserved native tissue architecture with minimal shrinkage compared to perfusion fixation, with superiority quantitatively shown by fractal analysis. In the crush model, hematoxylin-eosin, Luxol fast blue, and immunohistochemical staining of IVCT-prepared, freeze-substituted sections revealed axonal degeneration and regrowth. The close association between regenerating fibers and vascular structures, along with erythrocyte distribution, indicates a morphological link between nerves and blood vessels. Electrophysiological assessment using compound muscle action potentials and functional recovery measured by the sciatic functional index demonstrated restored nerve function at 28 days, consistent with histology. These findings suggest that IVCT is a useful method for analyzing peripheral nerve regeneration and vascular dynamics, thereby highlighting its potential as a novel approach in peripheral nerve research.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"59 2","pages":"75-88"},"PeriodicalIF":1.9,"publicationDate":"2026-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13122368/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147759257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0