Acta Histochemica Et Cytochemica最新文献

Three-Dimensional Culture of Glioblastoma Cells Using a Tissueoid Cell Culture System. 利用类组织细胞培养系统对胶质母细胞瘤细胞进行三维培养

IF 1.6 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2024-10-28 Epub Date: 2024-10-12 DOI: 10.1267/ahc.24-00043

Natsume Okamoto, Naoko Taniura, Takahisa Nakayama, Eri Tanaka, Yusuke Kageyama, Mai Noujima, Ryoji Kushima, Ken-Ichi Mukaisho

{"title":"Three-Dimensional Culture of Glioblastoma Cells Using a Tissueoid Cell Culture System.","authors":"Natsume Okamoto, Naoko Taniura, Takahisa Nakayama, Eri Tanaka, Yusuke Kageyama, Mai Noujima, Ryoji Kushima, Ken-Ichi Mukaisho","doi":"10.1267/ahc.24-00043","DOIUrl":"10.1267/ahc.24-00043","url":null,"abstract":"In classical cell culture techniques, cancer cells typically proliferate in a single layer by adhering to the undersurface of laboratory vessels. Consequently, concerns have been raised regarding the fidelity of the morphological and functional characteristics of these cultured cancer cells compared to those of their in vivo counterparts. Our previous studies have investigated various epithelial malignant tumors utilizing the Tissueoid cell culture system, a three-dimensional (3D) cultivation method employing Cellbed-a nonwoven sheet composed of high-purity silica fibers as a scaffold. In this investigation, we have achieved successful 3D culturing of glioblastoma cells (A172 and T98G), which are non-epithelial in nature. As such our focus is to juxtapose their morphological features against that of those cultivated via conventional two-dimensional (2D) methods. Our findings will be elucidated using immunostaining, immunofluorescence staining, and scanning electron microscopy, substantiated with accompanying imaging. Notably, cells cultured in the 3D environment exhibited distinct morphological attributes compared to those of their 2D counterparts, notably featuring pronounced cellular protrusions. We envisage the continued utilization of the 3D culture platform to facilitate diverse avenues of research, encompassing the exploration of novel therapeutic modalities for glioblastoma cells and beyond.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"57 5","pages":"149-155"},"PeriodicalIF":1.6,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11565224/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Localization of Both CD31- and Endomucin-Expressing Vessels in Mouse Dental Pulp. 小鼠牙髓中表达 CD31 和内切酶的血管的定位

IF 1.6 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2024-10-28 Epub Date: 2024-10-23 DOI: 10.1267/ahc.24-00009

Ryo Kambe, Keisuke Mitomo, Takatoshi Ikarashi, Mayuka Haketa, Kentaro Tashiro, Masahiro Furusawa, Takashi Muramatsu

{"title":"Localization of Both CD31- and Endomucin-Expressing Vessels in Mouse Dental Pulp.","authors":"Ryo Kambe, Keisuke Mitomo, Takatoshi Ikarashi, Mayuka Haketa, Kentaro Tashiro, Masahiro Furusawa, Takashi Muramatsu","doi":"10.1267/ahc.24-00009","DOIUrl":"10.1267/ahc.24-00009","url":null,"abstract":"We investigated the localization of both CD31- and endomucin-expressing vessels in mouse dental pulp to elucidate their relationship with dentin formation. The maxillae of C57BL/6 male mice (1, 4, 8, 12, and 56 weeks old) were fixed with 4% paraformaldehyde solution, and cryosections (12-μm-thick) were prepared. Immunofluorescence was performed using anti-CD31 and anti-endomucin antibodies, and calcein labeling was conducted to elucidate relationships with dentin formation. At 1 week, many CD31-expressing (CD31 (+)) and endomucin-expressing (endomucin (+)) vessels were observed throughout the dental papilla. At 4 weeks, CD31 (+) and endomucin (+) vessels decreased in the crown and increased in the root of dental pulp. At 12 weeks, CD31 (+) and endomucin (+) vessels were detected at the root apex, but not in coronal pulp. At 56 weeks, few CD31 (+) and endomucin (+) vessels were observed in dental pulp. Both CD31(+) and endomucin (+) vessels were detected directly beneath calcein-labeled dentin at all sites. These results suggest the presence of CD31 (+) and endomucin (+) vessels in dental pulp and their contribution to dentin formation.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"57 5","pages":"157-163"},"PeriodicalIF":1.6,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11565222/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fructose-bisphosphate Aldolase C Expression is Associated with Poor Prognosis and Stemness in Gastric Cancer. 果糖-二磷酸醛缩酶 C 的表达与胃癌的不良预后和干性有关

IF 1.6 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2024-10-28 Epub Date: 2024-10-23 DOI: 10.1267/ahc.24-00044

Akira Ishikawa, Yuki Shiwa, Narutaka Katsuya, Ryota Maruyama, Takafumi Fukui, Kazuya Kuraoka, Takahisa Suzuki, Hidehiko Takigawa, Shiro Oka, Wataru Yasui

{"title":"Fructose-bisphosphate Aldolase C Expression is Associated with Poor Prognosis and Stemness in Gastric Cancer.","authors":"Akira Ishikawa, Yuki Shiwa, Narutaka Katsuya, Ryota Maruyama, Takafumi Fukui, Kazuya Kuraoka, Takahisa Suzuki, Hidehiko Takigawa, Shiro Oka, Wataru Yasui","doi":"10.1267/ahc.24-00044","DOIUrl":"10.1267/ahc.24-00044","url":null,"abstract":"Gastric cancer (GC) is the third leading cause of cancer-related deaths in Japan, underscoring the urgent need for deeper insights into its pathogenesis. Spheroids provide a more realistic and versatile model for studying cancers and cancer stem cells (CSCs). While fructose-bisphosphate aldolase C (ALDOC) has been identified in colorectal cancer spheroids, its role in GC has remained largely unexplored. This study aimed to elucidate the role of ALDOC in GC by performing single-cell and functional analyses of GC spheroids and cell lines, along with immunohistochemistry of 127 GC samples to assess its correlation with CSC markers. Our single-cell analysis revealed upregulation of ALDOC in spheroids, with pseudotime analysis indicating that ALDOC-expressing cells were predominantly undifferentiated and co-expressed LGR5 and CD44. Further investigation into cell-cell interactions suggested that the stem cell state may be maintained by WNT, BMP, and EGF signaling. Functional assays demonstrated that ALDOC knockdown led to a marked reduction in the growth, invasiveness, and spheroid colony formation capacity of GC cell lines. Clinically, ALDOC was detected in the cytoplasm of 56.7% (72/127) of GC cases, and high ALDOC expression was significantly associated with poor overall survival (p < 0.01), and was an independent prognostic factor. Moreover, a significant association between ALDOC and CD44 expression in GC (p = 0.031). Conclusively, our findings identify ALDOC as a crucial prognostic marker and provide new insights into GC pathogenesis.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"57 5","pages":"165-174"},"PeriodicalIF":1.6,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11565221/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Hepatic Lipid Overload on Accelerated Hepatocyte Proliferation Promoted by HGF Expression via the SphK1/S1PR2 Pathway in MCD-diet Mouse Partial Hepatectomy. 肝脂质超载对 MCD-饮食小鼠部分肝切除术中通过 SphK1/S1PR2 通路促进 HGF 表达的肝细胞增殖的影响

IF 1.6 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2024-10-28 Epub Date: 2024-10-23 DOI: 10.1267/ahc.24-00046

Baljinnyam Lkham-Erdene, Narantsog Choijookhuu, Toshiki Kubota, Tomofumi Uto, Shuya Mitoma, Shinichiro Shirouzu, Takumi Ishizuka, Kengo Kai, Kazuhiro Higuchi, Kham Mo Aung, Jargal-Erdene Batmunkh, Katsuaki Sato, Yoshitaka Hishikawa

{"title":"Effect of Hepatic Lipid Overload on Accelerated Hepatocyte Proliferation Promoted by HGF Expression via the SphK1/S1PR2 Pathway in MCD-diet Mouse Partial Hepatectomy.","authors":"Baljinnyam Lkham-Erdene, Narantsog Choijookhuu, Toshiki Kubota, Tomofumi Uto, Shuya Mitoma, Shinichiro Shirouzu, Takumi Ishizuka, Kengo Kai, Kazuhiro Higuchi, Kham Mo Aung, Jargal-Erdene Batmunkh, Katsuaki Sato, Yoshitaka Hishikawa","doi":"10.1267/ahc.24-00046","DOIUrl":"10.1267/ahc.24-00046","url":null,"abstract":"Metabolic dysfunction-associated steatotic liver disease (MASLD) is becoming a major health problem worldwide. Liver regeneration is crucial for restoring liver function, and is regulated by extraordinary complex process, involving numerous factors under both physiologic and pathologic conditions. Sphingosine-1-phosphate (S1P), a bioactive sphingolipid synthesized by sphingosine kinase 1 (SphK1), plays an important role in liver function through S1P receptors (S1PRs)-expressing cells. In this study, we investigated the effect of lipid overload on hepatocyte proliferation in a mouse hepatic steatosis model induced by feeding a methionine- and choline-deficient (MCD) diet. After 50% partial hepatectomy (PHx), liver tissues were sampled at various timepoints and then analyzed by immunohistochemistry, oil Red-O staining, quantitative-polymerase chain reaction (qPCR), and flow cytometry. In mice fed the MCD-diet, significantly exacerbated hepatic steatosis and accelerated liver regeneration were observed. After PHx, hepatocyte proliferation peaked at 48 and 36 hr in the liver of chow- and MCD-diet fed mice, respectively. By contrast, increased expression of S1PR2 was observed in hepatic neutrophils and macrophages of MCD-diet fed mice. Flow cytometry and qPCR experiments demonstrated that levels of HGF and FGF2 released by neutrophils and macrophages were significantly higher in MCD-diet fed mice. In conclusion, hepatic lipid overload recruits Kupffer cells and neutrophils that release HGF and FGF2 via SphK1/S1PR2 activation to accelerate hepatocyte proliferation.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"57 5","pages":"175-188"},"PeriodicalIF":1.6,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11565223/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neutral Red Labeling: A Novel Vital Staining Method for Investigating Central and Peripheral Nervous System Lesions. 中性红标记：一种用于研究中枢和外周神经系统病变的新型活力染色法。

IF 1.6 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2024-08-29 Epub Date: 2024-08-23 DOI: 10.1267/ahc.24-00038

Reiji Yamazaki, Nobuhiko Ohno

{"title":"Neutral Red Labeling: A Novel Vital Staining Method for Investigating Central and Peripheral Nervous System Lesions.","authors":"Reiji Yamazaki, Nobuhiko Ohno","doi":"10.1267/ahc.24-00038","DOIUrl":"10.1267/ahc.24-00038","url":null,"abstract":"Multiple sclerosis, neuromyelitis optica, Guillain-Barré syndrome and chronic inflammatory demyelinating polyradiculoneuropathy are representative demyelinating diseases of the central and peripheral nervous system. Remyelination by myelin forming cells is important for functional recovery from the neurological deficits caused in the demyelinating diseases. Lysophosphatidylcholine-induced demyelination in mice is commonly used to identify and study the molecular pathways of demyelination and remyelination. However, detection of focally demyelinated lesions is difficult and usually requires sectioning of demyelinated lesions in tissues for microscopic analysis. In this review, we describe the development and application of a novel vital staining method for labeling demyelinated lesions using intraperitoneal injection of neutral red (NR) dye. NR labeling reduces the time and effort required to search for demyelinated lesions in tissues, and facilitates electron microscopic analysis of myelin structures. NR labeling also has the potential to contribute to the elucidation of pathologies in the central and peripheral nervous system and assist with identification of drug candidates that promote remyelination.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"57 4","pages":"131-135"},"PeriodicalIF":1.6,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11367148/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142124395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CpG Methylation of Receptor Activator NF-κB (RANK) Gene Promoter Region Delineates Senescence-Related Decrease of RANK Gene Expression. 受体活化因子 NF-κB (RANK) 基因启动子区域的 CpG 甲基化描述了衰老导致的 RANK 基因表达减少。

IF 1.6 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2024-08-29 Epub Date: 2024-08-23 DOI: 10.1267/ahc.24-00034

Riko Kitazawa, Ryuma Haraguchi, Yuki Murata, Yuki Takaoka, Sohei Kitazawa

{"title":"CpG Methylation of Receptor Activator NF-κB (RANK) Gene Promoter Region Delineates Senescence-Related Decrease of RANK Gene Expression.","authors":"Riko Kitazawa, Ryuma Haraguchi, Yuki Murata, Yuki Takaoka, Sohei Kitazawa","doi":"10.1267/ahc.24-00034","DOIUrl":"10.1267/ahc.24-00034","url":null,"abstract":"While the rapid decrease in estrogen is well known as the main cause of postmenopausal osteoporosis in women, the precise pathogenesis of senile osteoporosis in the elderly regardless of gender is largely unknown. The age-related epigenetic regulation of receptor activator NF-κB (RANK) gene expression was investigated with the use of a high-passaged mouse osteoclast progenitor cell line, RAW264.7, as an in vitro model of aging. In the RAW264.7 cells after repeated passages, receptor RANK expression was downregulated, resulting in decreased soluble RANK ligand (sRANKL)-induced osteoclastogenesis, expression of tartrate-resistant acid phosphatase-5b (TRAcP) and cathepsin K (CTSK). Methylation-specific PCR and bisulfite mapping revealed hypermethylation of CpG-loci located in the RANK gene promoter in multiple-passaged cells. ICON probe-mediated in situ assessment of methylated-cytosine at the CpG loci revealed an increase in the percentage of methylated RAW264.7 cells in the RANK gene in a passage-dependent manner. Conversely, upon treatment with demethylating agent 5-aza-2-deoxycytidine (5-aza-dC), high-passaged RAW264.7 cells displayed restored expression of the RANK gene, osteoclastogenesis, TRAcP and CTSK. Ex vivo cultures of splenic macrophages from young (10.5 W) and aged (12 M) mice also showed that CpG methylation was predominant in the aged animals, resulting in reduced RANK expression and osteoclastogenesis. Reduced RANK expression by age-related accumulation of DNA methylation, albeit in a limited population of osteoclast precursor cells, might be, at least in part, indicative of low-turnover bone characteristic of senile osteoporosis.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"57 4","pages":"137-147"},"PeriodicalIF":1.6,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11367149/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142124394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Purinosomes and Purine Metabolism in Mammalian Neural Development: A Review 哺乳动物神经发育过程中的嘌呤体和嘌呤代谢：综述

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2024-06-28 DOI: 10.1267/ahc.24-00027

Seiya Yamada, Tomoya Mizukoshi, Ayaka Sato, Shin-ichi Sakakibara

{"title":"Purinosomes and Purine Metabolism in Mammalian Neural Development: A Review","authors":"Seiya Yamada, Tomoya Mizukoshi, Ayaka Sato, Shin-ichi Sakakibara","doi":"10.1267/ahc.24-00027","DOIUrl":"https://doi.org/10.1267/ahc.24-00027","url":null,"abstract":"Neural stem/progenitor cells (NSPCs) in specific brain regions require precisely regulated metabolite production during critical development periods. Purines—vital components of DNA, RNA, and energy carriers like ATP and GTP—are crucial metabolites in brain development. Purine levels are tightly controlled through two pathways: de novo synthesis and salvage synthesis. Enzymes driving de novo pathway are assembled into a large multienzyme complex termed the “purinosome.” Here, we review purine metabolism and purinosomes as spatiotemporal regulators of neural development. Notably, around postnatal day 0 (P0) during mouse cortical development, purine synthesis transitions from the de novo pathway to the salvage pathway. Inhibiting the de novo pathway affects mTORC1 pathway and leads to specific forebrain malformations. In this review, we also explore the importance of protein-protein interactions of a newly identified NSPC protein—NACHT and WD repeat domain-containing 1 (Nwd1)—in purinosome formation. Reduced Nwd1 expression disrupts purinosome formation, impacting NSPC proliferation and neuronal migration, resulting in periventricular heterotopia. Nwd1 interacts directly with phosphoribosylaminoimidazole–succinocarboxamide synthetase (PAICS), an enzyme involved in de novo purine synthesis. We anticipate this review will be valuable for researchers investigating neural development, purine metabolism, and protein-protein interactions.\u0000","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"166 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141505499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of Retinaldehyde Dehydrogenases in the Pituitary Glands of Fetus and Adult Mice 胎鼠和成年小鼠垂体中视黄醛脱氢酶的表达

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2024-06-28 DOI: 10.1267/ahc.24-00018

Yanan Wei, Si Cheng, Takehiro Tsukada, Kotaro Horiguchi, Yoko Fujiwara, Ken Fujiwara

{"title":"Expression of Retinaldehyde Dehydrogenases in the Pituitary Glands of Fetus and Adult Mice","authors":"Yanan Wei, Si Cheng, Takehiro Tsukada, Kotaro Horiguchi, Yoko Fujiwara, Ken Fujiwara","doi":"10.1267/ahc.24-00018","DOIUrl":"https://doi.org/10.1267/ahc.24-00018","url":null,"abstract":"Retinoic acid (RA) plays a critical role in cell growth and tissue development. RA is synthesized from retinoids through oxidation processes by the retinaldehyde dehydrogenase (Raldh) family. However, the expression of Raldhs during pituitary development and the identification of Raldh-expressing cells in the adult pituitary have not been fully elucidated. Here, we performed in situ hybridization to localize the three Raldh isoforms (Raldh1-3) in fetal and adult mouse pituitary glands. The results showed that Raldh2 expression was observed in Rathke’s pouch from embryonic day 13.5 (E13.5), and this expression was sustained in the anterior lobe of the pituitary primordium from E15.5 to E17.5. In contrast, Raldh1 and Raldh3 were rarely detectable. Real-time PCR analysis revealed that Raldh2 was the predominant isoform expressed in the adult pituitary, although Raldh1 was also expressed to a lesser extent. In the adult pituitary, Raldh1-expressing cells were primarily observed in the posterior lobe. Raldh2-expressing cells were found in the marginal cell layer and parenchyma of the anterior lobe and were immunopositive for aldolase C (folliculostellate cells), but not for anterior pituitary hormones. These results suggest that RA is an important regulatory factor in the functions of the pituitary throughout its development in mice.\u0000","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"472 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141505502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated Spatial Multi-Omics Study of Postmortem Brains of Alzheimer’s Disease 阿尔茨海默氏症死后大脑的空间多指标综合研究

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2024-06-28 DOI: 10.1267/ahc.24-00025

Yumiko Toyama, Takashi Nirasawa, Maho Morishima, Yuko Saito, Kazuhiro Irie, Shigeo Murayama, Masaya Ikegawa

{"title":"Integrated Spatial Multi-Omics Study of Postmortem Brains of Alzheimer’s Disease","authors":"Yumiko Toyama, Takashi Nirasawa, Maho Morishima, Yuko Saito, Kazuhiro Irie, Shigeo Murayama, Masaya Ikegawa","doi":"10.1267/ahc.24-00025","DOIUrl":"https://doi.org/10.1267/ahc.24-00025","url":null,"abstract":"Pathological hallmark of Alzheimer’s disease (AD) is characterized by the accumulation and aggregation of amyloid β (Aβ) peptides into extracellular plaques of the brain. Clarification of the process of how soluble Aβ starts to assemble into amyloid fibrils is an essential step in elucidating the pathogenesis of AD. In our previous study, Aβ proteoforms including full-length Aβ40 and Aβ42/43 with N- and C-terminal truncated forms were visualized in postmortem brains from AD patients with matrix-assisted laser desorption/ionization-based mass spectrometry imaging (MALDI-MSI). In this study, Aβ proteoforms were consistently visualized by an updated protocol, and uncharacterized peptides such as Aβ1-29 and Aβ10-40 in AD brains were also visualized. To decipher neurotoxic effects of Aβ in patients’ brains, here we integrate liquid chromatography tandem mass spectrometry (LC-MS/MS) based shotgun proteomics with laser microdissection (LMD) excised tissue samples as well as direct tissue imaging with MALDI-MSI. With this approach, we have highlighted dynamic alterations of microtubule associating proteins (MAPs) including MAP1A, MAP1B and MAP2 as well as AD dominant proteins including APP, UCHL1, SNCA, and APOE. Of note, as lipid dysregulation has been implicated with AD pathology, we have challenged to integrate proteomics and lipid imaging for AD and control brain tissue. Spatial multi-omics is also valid to uncover molecular pathology of white matter as well as grey matter and leptomeningeal area, for example, by visualizing heme in patients’ postmortem brains.\u0000","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"166 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141505507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunohistochemical Study of Human Mitochondrial Ferritin in the Substantia Nigra Following Subarachnoid Hemorrhage 蛛网膜下腔出血后黑质下人体线粒体铁蛋白的免疫组化研究

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2024-05-10 DOI: 10.1267/ahc.24-00002

Shogo Takahata, Tomoko Kato, Daijiro Yanagisawa, Haruka Tsubaki, Zulzikry Hafiz Abu Bakar, Ken-ichi Mukaisho, Yasushi Itoh, Ikuo Tooyama

{"title":"Immunohistochemical Study of Human Mitochondrial Ferritin in the Substantia Nigra Following Subarachnoid Hemorrhage","authors":"Shogo Takahata, Tomoko Kato, Daijiro Yanagisawa, Haruka Tsubaki, Zulzikry Hafiz Abu Bakar, Ken-ichi Mukaisho, Yasushi Itoh, Ikuo Tooyama","doi":"10.1267/ahc.24-00002","DOIUrl":"https://doi.org/10.1267/ahc.24-00002","url":null,"abstract":"Mitochondrial ferritin (FtMt) is a novel ferritin that sequesters iron and plays a protective role against oxidative stress. FtMt shares a high homology with H-ferritin but is expressed only in the brain, heart, and testis. In the midbrain, FtMt expression is observed in the substantia nigra. FtMt plays a neuroprotective role in the pathology of neurodegenerative diseases such as Parkinson’s disease, where excessive iron induces oxidative stress, causing cell death. Herein, we investigated FtMt immunoreactivity in the brains of patients with subarachnoid hemorrhage (SAH). Double immunofluorescence labeling of tyrosine hydroxylase (TH) and FtMt showed high colocalization in the substantia nigra pars compacta (SNc) in control and SAH cases. However, in SAH cases, FtMt immunoreactivity was observed in some TH-negative neurons. Double immunofluorescence labeling of glial cell markers and FtMt showed no apparent colocalization. The number and ratio of FtMt-positive but TH-negative neurons significantly differed between the control and SAH groups. Prussian blue staining in SAH cases showed positive iron staining over a wide surface range and the substantia nigra. Thus, FtMt may be related to iron dynamics in the substantia nigra following subarachnoid hemorrhage.\u0000","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"14 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140926714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0