Acta Histochemica Et Cytochemica最新文献

Immunohistochemical Study on O-GlcNAcylation in Diabetic Pathologies: Molecular Mechanisms and Implications. o - glcn酰化在糖尿病病理中的免疫组织化学研究：分子机制和意义。

IF 1.6 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2025-06-24 Epub Date: 2025-06-18 DOI: 10.1267/ahc.25-00022

Yoshihiro Akimoto, Yuri Miura, Akihiko Kudo, Hiroki Tsumoto, Toshiyuki Fukutomi, Daisuke Sugahara, Tomio Arai, Yuko Chiba, Shinya Kaname, Kunimasa Yan, Hayato Kawakami, Tamao Endo

{"title":"Immunohistochemical Study on O-GlcNAcylation in Diabetic Pathologies: Molecular Mechanisms and Implications.","authors":"Yoshihiro Akimoto, Yuri Miura, Akihiko Kudo, Hiroki Tsumoto, Toshiyuki Fukutomi, Daisuke Sugahara, Tomio Arai, Yuko Chiba, Shinya Kaname, Kunimasa Yan, Hayato Kawakami, Tamao Endo","doi":"10.1267/ahc.25-00022","DOIUrl":"10.1267/ahc.25-00022","url":null,"abstract":"O-linked N-acetylglucosamine (O-GlcNAc) modification, known as O-GlcNAcylation, is a dynamic post-translational modification involving the addition of N-acetylglucosamine to serine or threonine residues. It has emerged as a critical regulator in diabetic pathophysiology. This review summarizes current research on the role of O-GlcNAcylation in hyperglycemia-induced cellular dysfunction, and focuses on vascular smooth muscle cells, renal cytoskeletal proteins, and diabetic complications in animal and human models. Studies reveal that hyperglycemia upregulates O-GlcNAc transferase activity, disrupting the interplay between glycosylation and phosphorylation, thereby impairing signaling pathways and exacerbating vascular proliferation and renal cytoskeletal disorganization. Notable findings include the imbalance of β-actin modifications in diabetic nephropathy, correlated with podocyte damage and glomerular abnormalities. By elucidating these mechanistic pathways, this review underscores the potential of O-GlcNAcylation as a biomarker and a therapeutic target. Future research should focus on tissue-specific effects and pharmacological strategies that mitigate diabetes-induced complications while preserving normal cellular functions.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"58 3","pages":"115-121"},"PeriodicalIF":1.6,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229784/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of circPVT1 Expression on Prostate Cancer Malignancy and Tumor Growth. circPVT1表达对前列腺癌恶性及肿瘤生长的影响

IF 1.6 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2025-06-24 Epub Date: 2025-06-18 DOI: 10.1267/ahc.25-00008

Miyaka Umemori, Kojiro Tashiro, Ayana Horiguchi, Fumihiko Urabe, Takahiro Kimura, Shun Sato, Takahashi Hiroyuki, Morito Kurata

{"title":"Impact of circPVT1 Expression on Prostate Cancer Malignancy and Tumor Growth.","authors":"Miyaka Umemori, Kojiro Tashiro, Ayana Horiguchi, Fumihiko Urabe, Takahiro Kimura, Shun Sato, Takahashi Hiroyuki, Morito Kurata","doi":"10.1267/ahc.25-00008","DOIUrl":"10.1267/ahc.25-00008","url":null,"abstract":"The long non-coding RNA PVT1 reportedly forms a circular RNA variant (circPVT1). As circPVT1 is expressed in various cancers and has been implicated in promoting cancer cell proliferation and tumor progression, it is considered a potential biomarker and therapeutic target. We previously confirmed that circPVT1 expression varies according to the Gleason pattern, a morphological indicator of malignancy in prostate cancer. In this study, we assessed the expression of circPVT1 using BaseScopeTM assay with prostate cancer tissues and evaluated the correlation with the Grade Group (based on Gleason pattern), an indicator used to morphologically evaluate the degree of malignancy of prostate cancer. The relationship between circPVT1 expression and tumor proliferation was evaluated using cells in which circPVT1 expression was suppressed using the clustered regularly interspaced short palindromic repeats (CRISPR)/RfxCas13d system. BaseScopeTM assay confirmed that circPVT1 expression was significantly higher in Grade Group 2-5 (intermediate- and high-grade groups) than Grade Group 1 (low-grade group). In vitro experiments using the CRISPR/RfxCas13d system showed that specific suppression of circPVT1 expression resulted in a significant reduction in the number of prostate cancer cells. The results of this study suggest that circPVT1 is involved in tumor growth in prostate cancer and may serve as a therapeutic target for moderately and highly malignant prostate cancers that express circPVT1.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"58 3","pages":"133-141"},"PeriodicalIF":1.6,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229786/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

α-Synuclein-Assembled Synaptic Vesicle Pools at the Presynaptic Terminal: A Study of α-Synuclein Function Using a Novel Mouse Model. α-突触核蛋白在突触前末端组装的突触囊泡池：α-突触核蛋白功能的新小鼠模型研究

IF 1.6 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2025-06-24 Epub Date: 2025-06-18 DOI: 10.1267/ahc.25-00017

Chigure Suzuki, Junji Yamaguchi, Isei Tanida, Yasuo Uchiyama

{"title":"α-Synuclein-Assembled Synaptic Vesicle Pools at the Presynaptic Terminal: A Study of α-Synuclein Function Using a Novel Mouse Model.","authors":"Chigure Suzuki, Junji Yamaguchi, Isei Tanida, Yasuo Uchiyama","doi":"10.1267/ahc.25-00017","DOIUrl":"10.1267/ahc.25-00017","url":null,"abstract":"α-Synuclein is the causative gene for PARK1 and PARK4 (heterozygous triplication of SNCA) and is associated with Parkinson's disease, where it localizes to presynaptic terminals in mature neurons. Beyond Parkinson's disease, α-synuclein has also been implicated in various other neuronal disorders. In vitro studies using purified α-synuclein protein have suggested it is involved in synaptic vesicle assembly. However, its physiological function and the ultrastructure of its localization sites in presynaptic terminals remain unclear. To address this, we generated transgenic mice overexpressing human α-synuclein tagged with mKate2 (hSNCA-mKate2 mice) to investigate its in vivo role in synaptic vesicle pool formation at presynaptic terminals. These mice showed normal growth and fertility, and even at 1-yr. old, they showed no motor dysfunction compared to their wild-type littermates. Additionally, no abnormal protein aggregates indicative of neurodegeneration were observed. In this review, we summarize recent findings on the in vivo role of α-synuclein within presynaptic terminals, utilizing hSNCA-mKate2 mice in combination with in-resin correlative light and electron microscopy, electron microscopy, and immunohistochemistry.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"58 3","pages":"107-114"},"PeriodicalIF":1.6,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229785/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

α-Synuclein-Assembled Synaptic Vesicle Pools at the Presynaptic Terminal: A Study of α-Synuclein Function Using a Novel Mouse Model. α-突触核蛋白在突触前末端组装的突触囊泡池：α-突触核蛋白功能的新小鼠模型研究

IF 1.6 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2025-06-24 Epub Date: 2025-06-18 DOI: 10.1267/ahc.25-00017

Chigure Suzuki, Junji Yamaguchi, Isei Tanida, Yasuo Uchiyama

{"title":"α-Synuclein-Assembled Synaptic Vesicle Pools at the Presynaptic Terminal: A Study of α-Synuclein Function Using a Novel Mouse Model.","authors":"Chigure Suzuki, Junji Yamaguchi, Isei Tanida, Yasuo Uchiyama","doi":"10.1267/ahc.25-00017","DOIUrl":"10.1267/ahc.25-00017","url":null,"abstract":"α-Synuclein is the causative gene for PARK1 and PARK4 (heterozygous triplication of SNCA) and is associated with Parkinson's disease, where it localizes to presynaptic terminals in mature neurons. Beyond Parkinson's disease, α-synuclein has also been implicated in various other neuronal disorders. In vitro studies using purified α-synuclein protein have suggested it is involved in synaptic vesicle assembly. However, its physiological function and the ultrastructure of its localization sites in presynaptic terminals remain unclear. To address this, we generated transgenic mice overexpressing human α-synuclein tagged with mKate2 (hSNCA-mKate2 mice) to investigate its in vivo role in synaptic vesicle pool formation at presynaptic terminals. These mice showed normal growth and fertility, and even at 1-yr. old, they showed no motor dysfunction compared to their wild-type littermates. Additionally, no abnormal protein aggregates indicative of neurodegeneration were observed. In this review, we summarize recent findings on the in vivo role of α-synuclein within presynaptic terminals, utilizing hSNCA-mKate2 mice in combination with in-resin correlative light and electron microscopy, electron microscopy, and immunohistochemistry.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"58 3","pages":"107-114"},"PeriodicalIF":1.6,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229788/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunohistochemical Study on O-GlcNAcylation in Diabetic Pathologies: Molecular Mechanisms and Implications. o - glcn酰化在糖尿病病理中的免疫组织化学研究：分子机制和意义。

IF 1.6 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2025-06-24 Epub Date: 2025-06-18 DOI: 10.1267/ahc.25-00022

Yoshihiro Akimoto, Yuri Miura, Akihiko Kudo, Hiroki Tsumoto, Toshiyuki Fukutomi, Daisuke Sugahara, Tomio Arai, Yuko Chiba, Shinya Kaname, Kunimasa Yan, Hayato Kawakami, Tamao Endo

{"title":"Immunohistochemical Study on O-GlcNAcylation in Diabetic Pathologies: Molecular Mechanisms and Implications.","authors":"Yoshihiro Akimoto, Yuri Miura, Akihiko Kudo, Hiroki Tsumoto, Toshiyuki Fukutomi, Daisuke Sugahara, Tomio Arai, Yuko Chiba, Shinya Kaname, Kunimasa Yan, Hayato Kawakami, Tamao Endo","doi":"10.1267/ahc.25-00022","DOIUrl":"10.1267/ahc.25-00022","url":null,"abstract":"O-linked N-acetylglucosamine (O-GlcNAc) modification, known as O-GlcNAcylation, is a dynamic post-translational modification involving the addition of N-acetylglucosamine to serine or threonine residues. It has emerged as a critical regulator in diabetic pathophysiology. This review summarizes current research on the role of O-GlcNAcylation in hyperglycemia-induced cellular dysfunction, and focuses on vascular smooth muscle cells, renal cytoskeletal proteins, and diabetic complications in animal and human models. Studies reveal that hyperglycemia upregulates O-GlcNAc transferase activity, disrupting the interplay between glycosylation and phosphorylation, thereby impairing signaling pathways and exacerbating vascular proliferation and renal cytoskeletal disorganization. Notable findings include the imbalance of β-actin modifications in diabetic nephropathy, correlated with podocyte damage and glomerular abnormalities. By elucidating these mechanistic pathways, this review underscores the potential of O-GlcNAcylation as a biomarker and a therapeutic target. Future research should focus on tissue-specific effects and pharmacological strategies that mitigate diabetes-induced complications while preserving normal cellular functions.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"58 3","pages":"115-121"},"PeriodicalIF":1.6,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229783/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PRP and Cotton-Like β-TCP/PLGA Fibers Enhance Bone Repair in Osteoporotic Vertebral Defects via Macrophage Modulation in Rats. PRP和棉样β-TCP/PLGA纤维通过巨噬细胞调节促进骨质疏松性椎体缺损的骨修复。

IF 1.6 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2025-06-24 Epub Date: 2025-06-18 DOI: 10.1267/ahc.24-00066

Yuichi Shimizu, Masateru Nagae, Hitoshi Tonomura, Hidenobu Ishibashi, Nobuaki Takeura, Takahiro Morita, Munehiro Sakata, Shunji Yamada, Masaki Tanaka, Kenji Takahashi

{"title":"PRP and Cotton-Like β-TCP/PLGA Fibers Enhance Bone Repair in Osteoporotic Vertebral Defects via Macrophage Modulation in Rats.","authors":"Yuichi Shimizu, Masateru Nagae, Hitoshi Tonomura, Hidenobu Ishibashi, Nobuaki Takeura, Takahiro Morita, Munehiro Sakata, Shunji Yamada, Masaki Tanaka, Kenji Takahashi","doi":"10.1267/ahc.24-00066","DOIUrl":"10.1267/ahc.24-00066","url":null,"abstract":"This study assessed the effectiveness of a combination of platelet-rich plasma (PRP) and β-tricalcium phosphate/polylactic-co-glycolic acid (β-TCP/PLGA) fibers in the treatment of osteoporotic vertebral defects in rats. Seventy-two female Sprague-Dawley rats subjected to ovariectomy to induce osteoporosis were divided into three groups to receive different treatments for critical bone defects created in the lumbar vertebrae. The PRP group received β-TCP/PLGA fibers infused with PRP, the control group received no material, and the other group received the same fibers infused with phosphate-buffered saline (PBS). Over a period of 12 weeks, bone regeneration, macrophage differentiation, and inflammatory responses were evaluated histologically. Compared to the PBS group, the PRP-treated group demonstrated significantly enhanced early stage bone formation, increased expression of osteogenic markers, and a favorable shift in macrophage activity from the M1 inflammatory phenotype to the M2 healing phenotype. These outcomes suggest that the combination of PRP and β-TCP/PLGA fibers not only effectively promotes bone repair under osteoporotic conditions but also modulates the immune response to facilitate better healing, indicating its potential as a beneficial surgical intervention for osteoporotic vertebral fractures.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"58 3","pages":"123-132"},"PeriodicalIF":1.6,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229787/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PRP and Cotton-Like β-TCP/PLGA Fibers Enhance Bone Repair in Osteoporotic Vertebral Defects via Macrophage Modulation in Rats. PRP和棉样β-TCP/PLGA纤维通过巨噬细胞调节促进骨质疏松性椎体缺损的骨修复。

IF 1.6 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2025-06-24 Epub Date: 2025-06-18 DOI: 10.1267/ahc.24-00066

Yuichi Shimizu, Masateru Nagae, Hitoshi Tonomura, Hidenobu Ishibashi, Nobuaki Takeura, Takahiro Morita, Munehiro Sakata, Shunji Yamada, Masaki Tanaka, Kenji Takahashi

{"title":"PRP and Cotton-Like β-TCP/PLGA Fibers Enhance Bone Repair in Osteoporotic Vertebral Defects via Macrophage Modulation in Rats.","authors":"Yuichi Shimizu, Masateru Nagae, Hitoshi Tonomura, Hidenobu Ishibashi, Nobuaki Takeura, Takahiro Morita, Munehiro Sakata, Shunji Yamada, Masaki Tanaka, Kenji Takahashi","doi":"10.1267/ahc.24-00066","DOIUrl":"10.1267/ahc.24-00066","url":null,"abstract":"This study assessed the effectiveness of a combination of platelet-rich plasma (PRP) and β-tricalcium phosphate/polylactic-co-glycolic acid (β-TCP/PLGA) fibers in the treatment of osteoporotic vertebral defects in rats. Seventy-two female Sprague-Dawley rats subjected to ovariectomy to induce osteoporosis were divided into three groups to receive different treatments for critical bone defects created in the lumbar vertebrae. The PRP group received β-TCP/PLGA fibers infused with PRP, the control group received no material, and the other group received the same fibers infused with phosphate-buffered saline (PBS). Over a period of 12 weeks, bone regeneration, macrophage differentiation, and inflammatory responses were evaluated histologically. Compared to the PBS group, the PRP-treated group demonstrated significantly enhanced early stage bone formation, increased expression of osteogenic markers, and a favorable shift in macrophage activity from the M1 inflammatory phenotype to the M2 healing phenotype. These outcomes suggest that the combination of PRP and β-TCP/PLGA fibers not only effectively promotes bone repair under osteoporotic conditions but also modulates the immune response to facilitate better healing, indicating its potential as a beneficial surgical intervention for osteoporotic vertebral fractures.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"58 3","pages":"123-132"},"PeriodicalIF":1.6,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229782/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of circPVT1 Expression on Prostate Cancer Malignancy and Tumor Growth. circPVT1表达对前列腺癌恶性及肿瘤生长的影响

IF 1.6 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2025-06-24 Epub Date: 2025-06-18 DOI: 10.1267/ahc.25-00008

Miyaka Umemori, Kojiro Tashiro, Ayana Horiguchi, Fumihiko Urabe, Takahiro Kimura, Shun Sato, Takahashi Hiroyuki, Morito Kurata

{"title":"Impact of circPVT1 Expression on Prostate Cancer Malignancy and Tumor Growth.","authors":"Miyaka Umemori, Kojiro Tashiro, Ayana Horiguchi, Fumihiko Urabe, Takahiro Kimura, Shun Sato, Takahashi Hiroyuki, Morito Kurata","doi":"10.1267/ahc.25-00008","DOIUrl":"10.1267/ahc.25-00008","url":null,"abstract":"The long non-coding RNA PVT1 reportedly forms a circular RNA variant (circPVT1). As circPVT1 is expressed in various cancers and has been implicated in promoting cancer cell proliferation and tumor progression, it is considered a potential biomarker and therapeutic target. We previously confirmed that circPVT1 expression varies according to the Gleason pattern, a morphological indicator of malignancy in prostate cancer. In this study, we assessed the expression of circPVT1 using BaseScopeTM assay with prostate cancer tissues and evaluated the correlation with the Grade Group (based on Gleason pattern), an indicator used to morphologically evaluate the degree of malignancy of prostate cancer. The relationship between circPVT1 expression and tumor proliferation was evaluated using cells in which circPVT1 expression was suppressed using the clustered regularly interspaced short palindromic repeats (CRISPR)/RfxCas13d system. BaseScopeTM assay confirmed that circPVT1 expression was significantly higher in Grade Group 2-5 (intermediate- and high-grade groups) than Grade Group 1 (low-grade group). In vitro experiments using the CRISPR/RfxCas13d system showed that specific suppression of circPVT1 expression resulted in a significant reduction in the number of prostate cancer cells. The results of this study suggest that circPVT1 is involved in tumor growth in prostate cancer and may serve as a therapeutic target for moderately and highly malignant prostate cancers that express circPVT1.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"58 3","pages":"133-141"},"PeriodicalIF":1.6,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229781/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Horizontal Imaging of Skin Tumors Using Advanced Fluorescence Techniques for Detecting Individual Malignant Cells. 利用先进的荧光技术检测单个恶性细胞的皮肤肿瘤水平成像。

IF 1.6 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2025-04-26 DOI: 10.1267/ahc.24-00059

Satoshi Yoshida, Ryosuke Kawakami, Yosuke Niko, Kazuki Yatsuzuka, Teruko Tsuda, Hideki Mori, Jun Muto, Ken Shiraishi, Takeshi Imamura, Yasuhiro Fujisawa, Masamoto Murakami

{"title":"Horizontal Imaging of Skin Tumors Using Advanced Fluorescence Techniques for Detecting Individual Malignant Cells.","authors":"Satoshi Yoshida, Ryosuke Kawakami, Yosuke Niko, Kazuki Yatsuzuka, Teruko Tsuda, Hideki Mori, Jun Muto, Ken Shiraishi, Takeshi Imamura, Yasuhiro Fujisawa, Masamoto Murakami","doi":"10.1267/ahc.24-00059","DOIUrl":"10.1267/ahc.24-00059","url":null,"abstract":"Conventional histopathological techniques, such as hematoxylin and eosin staining, are limited to 4-5 μm-thick tissue sections, restricting visualization to two-dimensional planes. Moreover, acquisition of three-dimensional horizontal images from the skin surface remains challenging, hindering precise assessment of tumor margins in skin lesions. This challenge is particularly pronounced in extramammary Paget's disease (EMPD), in which diffuse epidermal tumor cell spread complicates accurate evaluation of lesion extent. We hypothesized that combining horizontal sectioning with identification of individual tumor cells would enhance the determination of surgical margins. In this study, we developed a deep-imaging technique utilizing fluorescent solvatochromic dyes (LipiORDER® and HistoBright®) and two-photon microscopy to achieve high-resolution tumor margin visualization in EMPD. This technique enables identification of tumor cells in frozen and paraffin-embedded tissue blocks, as well as in live skin tissue under physiological conditions. Our novel approach holds substantial promise for improving the precision of surgical-margin assessment in EMPD and other cutaneous malignancies.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"58 2","pages":"81-92"},"PeriodicalIF":1.6,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12173634/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reflections on the Principles of Fixation in Histochemistry and Cytochemistry: With a Special Focus on Immunohistochemistry and In Situ Hybridization. 对组织化学和细胞化学固定原理的思考：以免疫组织化学和原位杂交为重点。

IF 1.6 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2025-04-26 Epub Date: 2025-04-09 DOI: 10.1267/ahc.25-00006

Takehiko Koji

{"title":"Reflections on the Principles of Fixation in Histochemistry and Cytochemistry: With a Special Focus on Immunohistochemistry and In Situ Hybridization.","authors":"Takehiko Koji","doi":"10.1267/ahc.25-00006","DOIUrl":"10.1267/ahc.25-00006","url":null,"abstract":"In the field of histochemistry and cytochemistry (histocytochemistry), fixation is a critical process for preserving biological structures and enabling accurate analysis. Fixation methods, broadly categorized into precipitating and cross-linking techniques, stabilize biomolecules such as proteins, sugars (carbohydrates) and nucleic acids, although lipids often require specific handling due to the loss during a routine procedure. Traditional staining methods have evolved into advanced techniques like immunohistochemistry (IHC) and in situ hybridization (ISH), which allow for precise analysis of the expression of specific molecules. IHC employs antibodies to visualize specific antigens, with fixation playing a vital role in maintaining antigen integrity. However, excessive fixation can mask epitopes, requiring antigen retrieval techniques to restore antigenicity. Microwave-induced retrieval, for instance, enhances staining efficacy while introducing further fixation by promoting molecular interactions. ISH, which targets nucleic acids with specific base sequences, is also sensitive to fixation conditions. Formaldehyde-based fixatives react variably with purines and pyrimidines, affecting hybridization efficiency with a probe. Positive controls like 28S rRNA help to standardize ISH across facilities, ensuring reproducible and reliable results. Variability in fixation protocols among institutions brings fatal defects in achieving consistent results. Shared standards or the use of robust controls can alleviate these issues, enhancing the accuracy and reliability of histocytochemical analyses for both research and clinical applications.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"58 2","pages":"31-43"},"PeriodicalIF":1.6,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12173635/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0