Acta Histochemica Et Cytochemica最新文献

Morphological Sex Reversal in the Sexually Dimorphic Nucleus of the Preoptic Area in the Hypothalamus Delineated by Calbindin D28k-Immunoreactive Cell Clusters in Y ^POS mice. Calbindin d28k -免疫反应细胞簇描绘的Y POS小鼠下丘脑视前区两性二态核的形态性别逆转。

IF 1.8 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2025-08-28 Epub Date: 2025-07-24 DOI: 10.1267/ahc.25-00020

Takashi Tanida, Toshifumi Yokoyama, Takayuki Nakajima, Honoka Kawai, Nobuhiko Hoshi, Mitsuhiro Kawata, Ken Ichi Matsuda

{"title":"Morphological Sex Reversal in the Sexually Dimorphic Nucleus of the Preoptic Area in the Hypothalamus Delineated by Calbindin D28k-Immunoreactive Cell Clusters in Y POS mice.","authors":"Takashi Tanida, Toshifumi Yokoyama, Takayuki Nakajima, Honoka Kawai, Nobuhiko Hoshi, Mitsuhiro Kawata, Ken Ichi Matsuda","doi":"10.1267/ahc.25-00020","DOIUrl":"10.1267/ahc.25-00020","url":null,"abstract":"Sex-determining region Y (Sry) triggers testis development in mammals, and the presence or absence of testicular secretion determines their sex-specific brain phenotype. Mice with Y chromosome replaced by that of Mus domesticus poschiavinus (Y POS ) frequently display sex reversal due to delayed Sry expression. However, brain sexual dimorphism under conditions of disorders of sex development remains unclear. Here, we report sex differences in the sexually dimorphic nucleus of the preoptic area, delineated by cells positive for calbindin D28k, a male-predominant neuronal marker (CALB-SDN), in Y POS mice. The mice were divided into females and males according to gonadal phenotype. Cells immunoreactive (ir) for calbindin D28k (CALB) were more extensively distributed in male Y POS mice, compared with females. The CALB-ir cell numbers in the CALB-SDN were significantly higher in Y POS males than in Y POS females, which had numbers comparable to wild type females. No left-right differences in CALB-ir cell numbers were observed in the CALB-SDN. Collectively, these results demonstrate that sexual dimorphism of the CALB-ir cell cluster in the CALB-SDN strongly correlates with the gonadal sex phenotype rather than with the chromosomal sex in the Y POS mice, suggesting the effect of testicular secretion on the brain sexual differentiation with aberrant Y-linked gene expression.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"58 4","pages":"153-160"},"PeriodicalIF":1.8,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12422814/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145038753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discoidin Domain Receptor 2 (DDR2) Promotes Prostate Cancer Progression in Cooperation with Collagen Remodeling. 盘状蛋白结构域受体2 （DDR2）与胶原重塑合作促进前列腺癌进展。

IF 1.8 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2025-08-28 Epub Date: 2025-07-17 DOI: 10.1267/ahc.25-00009

Mikoto Sagehashi, Kiyoshi Takagi, Ai Sato, Mio Yamaguchi-Tanaka, Yasuhiro Miki, Akihiro Ito, Takashi Suzuki

{"title":"Discoidin Domain Receptor 2 (DDR2) Promotes Prostate Cancer Progression in Cooperation with Collagen Remodeling.","authors":"Mikoto Sagehashi, Kiyoshi Takagi, Ai Sato, Mio Yamaguchi-Tanaka, Yasuhiro Miki, Akihiro Ito, Takashi Suzuki","doi":"10.1267/ahc.25-00009","DOIUrl":"10.1267/ahc.25-00009","url":null,"abstract":"Prostate cancer is one of the most common malignancies in men and remodeling of extracellular collagen, especially collagen type I immensely contributes to the progress of prostate cancer. Discoidin domain receptor 2 (DDR2) is a receptor of collagen type I and transmits intracellular signaling in not only normal cells but also malignant cells, facilitating tumor progression. However, clinical and biological significance of DDR2 has not been well examined in prostate cancer. We therefore immunolocalized DDR2 and collagen type I in 117 prostate carcinoma tissues and correlated their immunoreactivity with clinicopathological characteristics of prostate cancer. We also conducted in vitro experiments using human prostate cancer cell lines to confirm the findings from immunohistochemical study. DDR2 immunoreactivity was positively associated with an aggressive phenotype of prostate cancer, partially in association with dense collagen I tissues which consisted of thin fibers. In addition, DDR2 immunoreactivity was significantly correlated with adverse clinical outcomes of prostate cancer. In vitro experiments revealed that DDR2 promoted proliferation and migration of PC-3 and DU-145 prostate cancer cell lines. It is therefore speculated that DDR2 promoted prostate cancer progression by interacting with collagen I, serving as a potent prognostic factor in prostate cancer.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"58 4","pages":"143-152"},"PeriodicalIF":1.8,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12422815/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145038786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunohistochemical Study on O-GlcNAcylation in Diabetic Pathologies: Molecular Mechanisms and Implications. o - glcn酰化在糖尿病病理中的免疫组织化学研究：分子机制和意义。

IF 1.6 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2025-06-24 Epub Date: 2025-06-18 DOI: 10.1267/ahc.25-00022

Yoshihiro Akimoto, Yuri Miura, Akihiko Kudo, Hiroki Tsumoto, Toshiyuki Fukutomi, Daisuke Sugahara, Tomio Arai, Yuko Chiba, Shinya Kaname, Kunimasa Yan, Hayato Kawakami, Tamao Endo

{"title":"Immunohistochemical Study on O-GlcNAcylation in Diabetic Pathologies: Molecular Mechanisms and Implications.","authors":"Yoshihiro Akimoto, Yuri Miura, Akihiko Kudo, Hiroki Tsumoto, Toshiyuki Fukutomi, Daisuke Sugahara, Tomio Arai, Yuko Chiba, Shinya Kaname, Kunimasa Yan, Hayato Kawakami, Tamao Endo","doi":"10.1267/ahc.25-00022","DOIUrl":"10.1267/ahc.25-00022","url":null,"abstract":"O-linked N-acetylglucosamine (O-GlcNAc) modification, known as O-GlcNAcylation, is a dynamic post-translational modification involving the addition of N-acetylglucosamine to serine or threonine residues. It has emerged as a critical regulator in diabetic pathophysiology. This review summarizes current research on the role of O-GlcNAcylation in hyperglycemia-induced cellular dysfunction, and focuses on vascular smooth muscle cells, renal cytoskeletal proteins, and diabetic complications in animal and human models. Studies reveal that hyperglycemia upregulates O-GlcNAc transferase activity, disrupting the interplay between glycosylation and phosphorylation, thereby impairing signaling pathways and exacerbating vascular proliferation and renal cytoskeletal disorganization. Notable findings include the imbalance of β-actin modifications in diabetic nephropathy, correlated with podocyte damage and glomerular abnormalities. By elucidating these mechanistic pathways, this review underscores the potential of O-GlcNAcylation as a biomarker and a therapeutic target. Future research should focus on tissue-specific effects and pharmacological strategies that mitigate diabetes-induced complications while preserving normal cellular functions.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"58 3","pages":"115-121"},"PeriodicalIF":1.6,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229784/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of circPVT1 Expression on Prostate Cancer Malignancy and Tumor Growth. circPVT1表达对前列腺癌恶性及肿瘤生长的影响

IF 1.6 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2025-06-24 Epub Date: 2025-06-18 DOI: 10.1267/ahc.25-00008

Miyaka Umemori, Kojiro Tashiro, Ayana Horiguchi, Fumihiko Urabe, Takahiro Kimura, Shun Sato, Takahashi Hiroyuki, Morito Kurata

{"title":"Impact of circPVT1 Expression on Prostate Cancer Malignancy and Tumor Growth.","authors":"Miyaka Umemori, Kojiro Tashiro, Ayana Horiguchi, Fumihiko Urabe, Takahiro Kimura, Shun Sato, Takahashi Hiroyuki, Morito Kurata","doi":"10.1267/ahc.25-00008","DOIUrl":"10.1267/ahc.25-00008","url":null,"abstract":"The long non-coding RNA PVT1 reportedly forms a circular RNA variant (circPVT1). As circPVT1 is expressed in various cancers and has been implicated in promoting cancer cell proliferation and tumor progression, it is considered a potential biomarker and therapeutic target. We previously confirmed that circPVT1 expression varies according to the Gleason pattern, a morphological indicator of malignancy in prostate cancer. In this study, we assessed the expression of circPVT1 using BaseScopeTM assay with prostate cancer tissues and evaluated the correlation with the Grade Group (based on Gleason pattern), an indicator used to morphologically evaluate the degree of malignancy of prostate cancer. The relationship between circPVT1 expression and tumor proliferation was evaluated using cells in which circPVT1 expression was suppressed using the clustered regularly interspaced short palindromic repeats (CRISPR)/RfxCas13d system. BaseScopeTM assay confirmed that circPVT1 expression was significantly higher in Grade Group 2-5 (intermediate- and high-grade groups) than Grade Group 1 (low-grade group). In vitro experiments using the CRISPR/RfxCas13d system showed that specific suppression of circPVT1 expression resulted in a significant reduction in the number of prostate cancer cells. The results of this study suggest that circPVT1 is involved in tumor growth in prostate cancer and may serve as a therapeutic target for moderately and highly malignant prostate cancers that express circPVT1.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"58 3","pages":"133-141"},"PeriodicalIF":1.6,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229786/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

α-Synuclein-Assembled Synaptic Vesicle Pools at the Presynaptic Terminal: A Study of α-Synuclein Function Using a Novel Mouse Model. α-突触核蛋白在突触前末端组装的突触囊泡池：α-突触核蛋白功能的新小鼠模型研究

IF 1.6 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2025-06-24 Epub Date: 2025-06-18 DOI: 10.1267/ahc.25-00017

Chigure Suzuki, Junji Yamaguchi, Isei Tanida, Yasuo Uchiyama

{"title":"α-Synuclein-Assembled Synaptic Vesicle Pools at the Presynaptic Terminal: A Study of α-Synuclein Function Using a Novel Mouse Model.","authors":"Chigure Suzuki, Junji Yamaguchi, Isei Tanida, Yasuo Uchiyama","doi":"10.1267/ahc.25-00017","DOIUrl":"10.1267/ahc.25-00017","url":null,"abstract":"α-Synuclein is the causative gene for PARK1 and PARK4 (heterozygous triplication of SNCA) and is associated with Parkinson's disease, where it localizes to presynaptic terminals in mature neurons. Beyond Parkinson's disease, α-synuclein has also been implicated in various other neuronal disorders. In vitro studies using purified α-synuclein protein have suggested it is involved in synaptic vesicle assembly. However, its physiological function and the ultrastructure of its localization sites in presynaptic terminals remain unclear. To address this, we generated transgenic mice overexpressing human α-synuclein tagged with mKate2 (hSNCA-mKate2 mice) to investigate its in vivo role in synaptic vesicle pool formation at presynaptic terminals. These mice showed normal growth and fertility, and even at 1-yr. old, they showed no motor dysfunction compared to their wild-type littermates. Additionally, no abnormal protein aggregates indicative of neurodegeneration were observed. In this review, we summarize recent findings on the in vivo role of α-synuclein within presynaptic terminals, utilizing hSNCA-mKate2 mice in combination with in-resin correlative light and electron microscopy, electron microscopy, and immunohistochemistry.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"58 3","pages":"107-114"},"PeriodicalIF":1.6,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229785/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

α-Synuclein-Assembled Synaptic Vesicle Pools at the Presynaptic Terminal: A Study of α-Synuclein Function Using a Novel Mouse Model. α-突触核蛋白在突触前末端组装的突触囊泡池：α-突触核蛋白功能的新小鼠模型研究

IF 1.6 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2025-06-24 Epub Date: 2025-06-18 DOI: 10.1267/ahc.25-00017

Chigure Suzuki, Junji Yamaguchi, Isei Tanida, Yasuo Uchiyama

{"title":"α-Synuclein-Assembled Synaptic Vesicle Pools at the Presynaptic Terminal: A Study of α-Synuclein Function Using a Novel Mouse Model.","authors":"Chigure Suzuki, Junji Yamaguchi, Isei Tanida, Yasuo Uchiyama","doi":"10.1267/ahc.25-00017","DOIUrl":"10.1267/ahc.25-00017","url":null,"abstract":"α-Synuclein is the causative gene for PARK1 and PARK4 (heterozygous triplication of SNCA) and is associated with Parkinson's disease, where it localizes to presynaptic terminals in mature neurons. Beyond Parkinson's disease, α-synuclein has also been implicated in various other neuronal disorders. In vitro studies using purified α-synuclein protein have suggested it is involved in synaptic vesicle assembly. However, its physiological function and the ultrastructure of its localization sites in presynaptic terminals remain unclear. To address this, we generated transgenic mice overexpressing human α-synuclein tagged with mKate2 (hSNCA-mKate2 mice) to investigate its in vivo role in synaptic vesicle pool formation at presynaptic terminals. These mice showed normal growth and fertility, and even at 1-yr. old, they showed no motor dysfunction compared to their wild-type littermates. Additionally, no abnormal protein aggregates indicative of neurodegeneration were observed. In this review, we summarize recent findings on the in vivo role of α-synuclein within presynaptic terminals, utilizing hSNCA-mKate2 mice in combination with in-resin correlative light and electron microscopy, electron microscopy, and immunohistochemistry.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"58 3","pages":"107-114"},"PeriodicalIF":1.6,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229788/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunohistochemical Study on O-GlcNAcylation in Diabetic Pathologies: Molecular Mechanisms and Implications. o - glcn酰化在糖尿病病理中的免疫组织化学研究：分子机制和意义。

IF 1.6 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2025-06-24 Epub Date: 2025-06-18 DOI: 10.1267/ahc.25-00022

Yoshihiro Akimoto, Yuri Miura, Akihiko Kudo, Hiroki Tsumoto, Toshiyuki Fukutomi, Daisuke Sugahara, Tomio Arai, Yuko Chiba, Shinya Kaname, Kunimasa Yan, Hayato Kawakami, Tamao Endo

{"title":"Immunohistochemical Study on O-GlcNAcylation in Diabetic Pathologies: Molecular Mechanisms and Implications.","authors":"Yoshihiro Akimoto, Yuri Miura, Akihiko Kudo, Hiroki Tsumoto, Toshiyuki Fukutomi, Daisuke Sugahara, Tomio Arai, Yuko Chiba, Shinya Kaname, Kunimasa Yan, Hayato Kawakami, Tamao Endo","doi":"10.1267/ahc.25-00022","DOIUrl":"10.1267/ahc.25-00022","url":null,"abstract":"O-linked N-acetylglucosamine (O-GlcNAc) modification, known as O-GlcNAcylation, is a dynamic post-translational modification involving the addition of N-acetylglucosamine to serine or threonine residues. It has emerged as a critical regulator in diabetic pathophysiology. This review summarizes current research on the role of O-GlcNAcylation in hyperglycemia-induced cellular dysfunction, and focuses on vascular smooth muscle cells, renal cytoskeletal proteins, and diabetic complications in animal and human models. Studies reveal that hyperglycemia upregulates O-GlcNAc transferase activity, disrupting the interplay between glycosylation and phosphorylation, thereby impairing signaling pathways and exacerbating vascular proliferation and renal cytoskeletal disorganization. Notable findings include the imbalance of β-actin modifications in diabetic nephropathy, correlated with podocyte damage and glomerular abnormalities. By elucidating these mechanistic pathways, this review underscores the potential of O-GlcNAcylation as a biomarker and a therapeutic target. Future research should focus on tissue-specific effects and pharmacological strategies that mitigate diabetes-induced complications while preserving normal cellular functions.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"58 3","pages":"115-121"},"PeriodicalIF":1.6,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229783/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PRP and Cotton-Like β-TCP/PLGA Fibers Enhance Bone Repair in Osteoporotic Vertebral Defects via Macrophage Modulation in Rats. PRP和棉样β-TCP/PLGA纤维通过巨噬细胞调节促进骨质疏松性椎体缺损的骨修复。

IF 1.6 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2025-06-24 Epub Date: 2025-06-18 DOI: 10.1267/ahc.24-00066

Yuichi Shimizu, Masateru Nagae, Hitoshi Tonomura, Hidenobu Ishibashi, Nobuaki Takeura, Takahiro Morita, Munehiro Sakata, Shunji Yamada, Masaki Tanaka, Kenji Takahashi

{"title":"PRP and Cotton-Like β-TCP/PLGA Fibers Enhance Bone Repair in Osteoporotic Vertebral Defects via Macrophage Modulation in Rats.","authors":"Yuichi Shimizu, Masateru Nagae, Hitoshi Tonomura, Hidenobu Ishibashi, Nobuaki Takeura, Takahiro Morita, Munehiro Sakata, Shunji Yamada, Masaki Tanaka, Kenji Takahashi","doi":"10.1267/ahc.24-00066","DOIUrl":"10.1267/ahc.24-00066","url":null,"abstract":"This study assessed the effectiveness of a combination of platelet-rich plasma (PRP) and β-tricalcium phosphate/polylactic-co-glycolic acid (β-TCP/PLGA) fibers in the treatment of osteoporotic vertebral defects in rats. Seventy-two female Sprague-Dawley rats subjected to ovariectomy to induce osteoporosis were divided into three groups to receive different treatments for critical bone defects created in the lumbar vertebrae. The PRP group received β-TCP/PLGA fibers infused with PRP, the control group received no material, and the other group received the same fibers infused with phosphate-buffered saline (PBS). Over a period of 12 weeks, bone regeneration, macrophage differentiation, and inflammatory responses were evaluated histologically. Compared to the PBS group, the PRP-treated group demonstrated significantly enhanced early stage bone formation, increased expression of osteogenic markers, and a favorable shift in macrophage activity from the M1 inflammatory phenotype to the M2 healing phenotype. These outcomes suggest that the combination of PRP and β-TCP/PLGA fibers not only effectively promotes bone repair under osteoporotic conditions but also modulates the immune response to facilitate better healing, indicating its potential as a beneficial surgical intervention for osteoporotic vertebral fractures.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"58 3","pages":"123-132"},"PeriodicalIF":1.6,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229787/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PRP and Cotton-Like β-TCP/PLGA Fibers Enhance Bone Repair in Osteoporotic Vertebral Defects via Macrophage Modulation in Rats. PRP和棉样β-TCP/PLGA纤维通过巨噬细胞调节促进骨质疏松性椎体缺损的骨修复。

IF 1.6 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2025-06-24 Epub Date: 2025-06-18 DOI: 10.1267/ahc.24-00066

Yuichi Shimizu, Masateru Nagae, Hitoshi Tonomura, Hidenobu Ishibashi, Nobuaki Takeura, Takahiro Morita, Munehiro Sakata, Shunji Yamada, Masaki Tanaka, Kenji Takahashi

{"title":"PRP and Cotton-Like β-TCP/PLGA Fibers Enhance Bone Repair in Osteoporotic Vertebral Defects via Macrophage Modulation in Rats.","authors":"Yuichi Shimizu, Masateru Nagae, Hitoshi Tonomura, Hidenobu Ishibashi, Nobuaki Takeura, Takahiro Morita, Munehiro Sakata, Shunji Yamada, Masaki Tanaka, Kenji Takahashi","doi":"10.1267/ahc.24-00066","DOIUrl":"10.1267/ahc.24-00066","url":null,"abstract":"This study assessed the effectiveness of a combination of platelet-rich plasma (PRP) and β-tricalcium phosphate/polylactic-co-glycolic acid (β-TCP/PLGA) fibers in the treatment of osteoporotic vertebral defects in rats. Seventy-two female Sprague-Dawley rats subjected to ovariectomy to induce osteoporosis were divided into three groups to receive different treatments for critical bone defects created in the lumbar vertebrae. The PRP group received β-TCP/PLGA fibers infused with PRP, the control group received no material, and the other group received the same fibers infused with phosphate-buffered saline (PBS). Over a period of 12 weeks, bone regeneration, macrophage differentiation, and inflammatory responses were evaluated histologically. Compared to the PBS group, the PRP-treated group demonstrated significantly enhanced early stage bone formation, increased expression of osteogenic markers, and a favorable shift in macrophage activity from the M1 inflammatory phenotype to the M2 healing phenotype. These outcomes suggest that the combination of PRP and β-TCP/PLGA fibers not only effectively promotes bone repair under osteoporotic conditions but also modulates the immune response to facilitate better healing, indicating its potential as a beneficial surgical intervention for osteoporotic vertebral fractures.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"58 3","pages":"123-132"},"PeriodicalIF":1.6,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229782/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of circPVT1 Expression on Prostate Cancer Malignancy and Tumor Growth. circPVT1表达对前列腺癌恶性及肿瘤生长的影响

IF 1.6 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2025-06-24 Epub Date: 2025-06-18 DOI: 10.1267/ahc.25-00008

Miyaka Umemori, Kojiro Tashiro, Ayana Horiguchi, Fumihiko Urabe, Takahiro Kimura, Shun Sato, Takahashi Hiroyuki, Morito Kurata

{"title":"Impact of circPVT1 Expression on Prostate Cancer Malignancy and Tumor Growth.","authors":"Miyaka Umemori, Kojiro Tashiro, Ayana Horiguchi, Fumihiko Urabe, Takahiro Kimura, Shun Sato, Takahashi Hiroyuki, Morito Kurata","doi":"10.1267/ahc.25-00008","DOIUrl":"10.1267/ahc.25-00008","url":null,"abstract":"The long non-coding RNA PVT1 reportedly forms a circular RNA variant (circPVT1). As circPVT1 is expressed in various cancers and has been implicated in promoting cancer cell proliferation and tumor progression, it is considered a potential biomarker and therapeutic target. We previously confirmed that circPVT1 expression varies according to the Gleason pattern, a morphological indicator of malignancy in prostate cancer. In this study, we assessed the expression of circPVT1 using BaseScopeTM assay with prostate cancer tissues and evaluated the correlation with the Grade Group (based on Gleason pattern), an indicator used to morphologically evaluate the degree of malignancy of prostate cancer. The relationship between circPVT1 expression and tumor proliferation was evaluated using cells in which circPVT1 expression was suppressed using the clustered regularly interspaced short palindromic repeats (CRISPR)/RfxCas13d system. BaseScopeTM assay confirmed that circPVT1 expression was significantly higher in Grade Group 2-5 (intermediate- and high-grade groups) than Grade Group 1 (low-grade group). In vitro experiments using the CRISPR/RfxCas13d system showed that specific suppression of circPVT1 expression resulted in a significant reduction in the number of prostate cancer cells. The results of this study suggest that circPVT1 is involved in tumor growth in prostate cancer and may serve as a therapeutic target for moderately and highly malignant prostate cancers that express circPVT1.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"58 3","pages":"133-141"},"PeriodicalIF":1.6,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229781/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0