Acta Histochemica Et Cytochemica最新文献_第10页

Immunohistochemical Pharmacokinetics of the Anti-diabetes Drug Alogliptin in Rat Kidney and Liver. 大鼠肾脏和肝脏中抗糖尿病药物阿格列汀的免疫组织化学药代动力学

IF 1.6 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2020-06-26 Epub Date: 2020-05-29 DOI: 10.1267/ahc.19036

Yutaro Yamamoto, Yuta Yamamoto, Tetsuya Saita, Daisuke Hira, Takahito Chijiwa, Masashi Shin

{"title":"Immunohistochemical Pharmacokinetics of the Anti-diabetes Drug Alogliptin in Rat Kidney and Liver.","authors":"Yutaro Yamamoto, Yuta Yamamoto, Tetsuya Saita, Daisuke Hira, Takahito Chijiwa, Masashi Shin","doi":"10.1267/ahc.19036","DOIUrl":"10.1267/ahc.19036","url":null,"abstract":"Alogliptin is one of a new class of therapeutic agents for type 2 diabetes called dipeptidyl peptidase-4 inhibitors. Here, we used immunohistochemistry to investigate the pharmacokinetics of alogliptin at the cell and tissue levels in the rat kidney and liver. One hour after alogliptin administration, the most noticeable immunoreactivity in the kidney was a moderate-to-strong staining in proximal tubule S3 segment epithelial cells. On the other hand, immunostaining was found only in the microvilli of S1 and S2 segment cells. Immunoreactivity was also observed in the glomerulus and distal tubules. Positive cells and almost negative cells coexisted in the collecting ducts. Twenty-four hours after administration, moderate immunostaining remained in the S3 segment but staining in other regions had almost disappeared. In the liver 1 hr after administration, hepatocyte staining differed in the hepatic lobule, with zone III being stronger than zone I. Immunostaining had almost disappeared 24 hr after administration. These findings suggest that alogliptin reabsorption at the kidney and uptake at the hepatocyte vary from region to region and that one or more types of transporter are involved in these processes. In addition, long-term alogliptin use may cause the drug to accumulate in S3 segment, leading to adverse events.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2020-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7322161/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38118483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of Novel c-Kit-expressing Smooth Muscle Cells in Murine Cecum. 新型表达c- kit的小鼠盲肠平滑肌细胞的研究。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2020-04-28 Epub Date: 2020-04-09 DOI: 10.1267/ahc.20003

Satoshi Iino, Kazuhide Horiguchi, Satomi Horiguchi

引用次数: 1

New Insights into the Pathogenesis of Diabetic Nephropathy: Proximal Renal Tubules Are Primary Target of Oxidative Stress in Diabetic Kidney. 糖尿病肾病发病机制的新见解:近端肾小管是糖尿病肾脏氧化应激的主要靶点。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2020-04-28 Epub Date: 2020-04-25 DOI: 10.1267/ahc.20008

Ryuma Haraguchi, Yukihiro Kohara, Kanako Matsubayashi, Riko Kitazawa, Sohei Kitazawa

{"title":"New Insights into the Pathogenesis of Diabetic Nephropathy: Proximal Renal Tubules Are Primary Target of Oxidative Stress in Diabetic Kidney.","authors":"Ryuma Haraguchi, Yukihiro Kohara, Kanako Matsubayashi, Riko Kitazawa, Sohei Kitazawa","doi":"10.1267/ahc.20008","DOIUrl":"https://doi.org/10.1267/ahc.20008","url":null,"abstract":"Diabetic nephropathy is a major source of end-stage renal failure, affecting about one-third cases of diabetes mellitus. It has long been accepted that diabetic nephropathy is mainly characterized by glomerular defects, while clinical observations have implied that renal tubular damage is closely linked to kidney dysfunction at the early stages of diabetic nephropathy. In this study, we conducted pathohistological analyses focusing on renal tubular lesions in the early-stage diabetic kidney with the use of a streptozotocin (STZ)-induced diabetes mellitus mouse model. The results revealed that histological alterations in renal tubules, shown by a vacuolar nucleic structure, accumulations of PAS-positive substance, and accelerated restoration stress, occur initially without the presence of glomerular lesions in the early-stage diabetic kidney, and that these tubular defects are localized mainly in proximal renal tubules. Moreover, enhanced expression of RAGE, suggesting an aberrant activation of AGEs-RAGE signaling pathway, and accumulation of oxidative modified mitochondria through the impaired autophagy/lysosome system, were also seen in the damaged diabetic proximal renal tubules. Our findings indicate that proximal tubular defects are the initial pathological events increasingly linked to the progression of diabetic nephropathy, and that controlling renal tubular damage could be an effective therapeutic strategy for the clinical treatment of diabetic nephropathy.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2020-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1267/ahc.20008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37936912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

Codependency of Metabolism and Epigenetics Drives Cancer Progression: A Review. 新陈代谢与表观遗传学的相互依存关系推动癌症进展：综述。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2020-02-28 Epub Date: 2020-02-26 DOI: 10.1267/ahc.20002

Kenta Masui, Mio Harachi, Webster K Cavenee, Paul S Mischel, Noriyuki Shibata

{"title":"Codependency of Metabolism and Epigenetics Drives Cancer Progression: A Review.","authors":"Kenta Masui, Mio Harachi, Webster K Cavenee, Paul S Mischel, Noriyuki Shibata","doi":"10.1267/ahc.20002","DOIUrl":"10.1267/ahc.20002","url":null,"abstract":"Cancer is widely considered to be a set of genetic diseases that are currently classified by tissue and cell type of origin and, increasingly, by its molecular characteristics. This latter aspect is based primarily upon oncogene gains, tumor suppressor losses, and associated transcriptional profiles. However, cancers are also characterized by profound alterations in cellular metabolism and epigenetic landscape. It is particularly noteworthy that cancer-causing genomic defects not only activate cell cycle progression, but regulate the opportunistic uptake and utilization of nutrients, effectively enabling tumors to maximize growth and drug resistance in changing tissue and systemic microenvironments. Shifts in chromatin architecture are central to this dynamic behavior. Further, changes in nutrient uptake and utilization directly affect chromatin structure. In this review, we describe a set of recent discoveries of metabolic and epigenetic reprogramming in cancer, and especially focus on the genomically well-characterized brain tumor, glioblastoma. Further, we discuss a new mode of metabolic regulation driven by epigenetic mechanisms, that enables cancer cells to autonomously activate iron metabolism for their survival. Together, these underscore the integration of genetic mutations with metabolic reprogramming and epigenetic shifts in cancer, suggesting a new means to identifying patient subsets suitable for specific precision therapeutics.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2020-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7076272/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37762132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Incremental Growth Lines in Mouse Molar Dentin Represent 8-hr Ultradian Rhythm. 小鼠磨牙本质的增量生长线代表8小时的超昼夜节律。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2019-12-27 Epub Date: 2019-12-20 DOI: 10.1267/ahc.19017

Ryutaro Ono, Nobuya Koike, Hitoshi Inokawa, Yoshiki Tsuchiya, Yasuhiro Umemura, Toshiro Yamamoto, Narisato Kanamura, Kazuhiro Yagita

{"title":"Incremental Growth Lines in Mouse Molar Dentin Represent 8-hr Ultradian Rhythm.","authors":"Ryutaro Ono, Nobuya Koike, Hitoshi Inokawa, Yoshiki Tsuchiya, Yasuhiro Umemura, Toshiro Yamamoto, Narisato Kanamura, Kazuhiro Yagita","doi":"10.1267/ahc.19017","DOIUrl":"https://doi.org/10.1267/ahc.19017","url":null,"abstract":"Rhythmic incremental growth lines occur in dental hard tissues of vertebrates, and dentinogenesis in rodent incisors is suggested to be controlled by the 24-hr circadian clock. Rodent incisors continue to grow throughout the animal's life; however, similar to human teeth, rodent molars stop growing after crown formation. This similarity suggests that the mouse molar is an excellent model to understand the molecular mechanisms underlying growth of human teeth. However, not much is known about the rhythmic dentinogenesis in mouse molars. Here, we investigated the incremental growth lines in mouse molar dentin using tetracycline as the growth marker. The incremental growth lines were observed to be generated at approximately 8-hr intervals in wild-type mice housed under 12:12 hr light-dark conditions. Moreover, the 8-hr rhythmic increments persisted in the wild-type and Bmal1-/- mice housed in constant darkness, where Bmal1-/- mice become behaviorally arrhythmic. These results revealed that the dentinogenesis in mouse molars underlie the ultradian rhythms with around 8-hr periodicity. Further, the circadian clock does not seem to be involved in this process, providing new insight into the mechanisms involved in the tooth growth.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2019-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1267/ahc.19017","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37594548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Localization and Accumulation Studies of Dacomitinib in Rat Intestines and Skin by Immunohistochemistry. 免疫组织化学研究达科替尼在大鼠肠道和皮肤中的定位和蓄积。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2019-12-27 Epub Date: 2019-12-25 DOI: 10.1267/ahc.19031

Yutaro Yamamoto, Tetsuya Saita, Asuki Oka, Hiroto Kataoka, Masashi Shin

{"title":"Localization and Accumulation Studies of Dacomitinib in Rat Intestines and Skin by Immunohistochemistry.","authors":"Yutaro Yamamoto, Tetsuya Saita, Asuki Oka, Hiroto Kataoka, Masashi Shin","doi":"10.1267/ahc.19031","DOIUrl":"https://doi.org/10.1267/ahc.19031","url":null,"abstract":"Dacomitinib, a second-generation tyrosine kinase inhibitor, was irreversible inhibitor forming covalent bonds with the kinase domains of EGFR and other ErbB family receptors. Dacomitinib has been approved for the treatment of locally advanced or metastatic non-small cell lung cancer. In this study, we aimed to develop an immunohistochemistry to detect dacomitinib-ErbB family receptor conjugates. Immunostaining was performed in rat intestine and skin tissues after oral administration of dacomitinib. Following a single oral dose of dacomitinib, strong staining was observed after 24 hr in the ileum and colon, with only slight staining in the duodenum and jejunum. In the skin, strong staining was observed in the epidermis, hair follicles, and sebaceous glands. Moreover, significant amounts of dacomitinib remained for up to 72 hr post-administration in the ileum, colon, and skin. This report is the first to elucidate the localization and accumulation of dacomitinib in the rat intestine and skin and should be valuable during efforts to clarify the mechanism dacomitinib-induced diarrhea or skin toxicities.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2019-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1267/ahc.19031","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37594549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Hypothalamic Kisspeptin Expression in Hyperandrogenic Female Rats and Aging Rats 高雄激素雌性大鼠和衰老大鼠下丘脑Kisspeptin的表达

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2019-09-26 DOI: 10.1267/ahc.19013

K. Iwata, Yuyu Kunimura, H. Ozawa

{"title":"Hypothalamic Kisspeptin Expression in Hyperandrogenic Female Rats and Aging Rats","authors":"K. Iwata, Yuyu Kunimura, H. Ozawa","doi":"10.1267/ahc.19013","DOIUrl":"https://doi.org/10.1267/ahc.19013","url":null,"abstract":"Hypothalamic kisspeptin neurons stimulate gonadotropin-releasing hormone (GnRH) and luteinizing hormone (LH) release. Kisspeptin neurons in the anteroventral periventricular nucleus (AVPV) of rats induce an LH surge for ovulation, and those in the arcuate nucleus (ARC) regulate pulsatile LH secretion for follicle development and spermatogenesis. Dysfunction of kisspeptin neurons thus reduces the reproductive function. This review focuses on the effect of androgen or aging on kisspeptin expression in rats. Although androgen directly suppresses ARC kisspeptin neurons in female rats, the AVPV kisspeptin neurons are hardly affected. In rats, plasma LH concentrations decrease in both sexes with aging, and ARC kisspeptin expression also decreases in old rats compared with young rats. In addition, kisspeptin neurons may be associated with hyperprolactinemia in old female rats because they are known to release prolactin through hypothalamic tuberoinfundibular dopaminergic (TIDA) neurons. Hypothalamic kisspeptin neurons are thus the main regulator to secrete LH, and inhibition of kisspeptin expression leads to various kinds of reproductive dysfunction.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2019-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1267/ahc.19013","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42066040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Reversible Conversion among Subtypes of Salivary Gland Duct Cells as Identified by Production of a Variety of Bioactive Polypeptides 唾液腺导管细胞亚型间的可逆转化是通过多种生物活性多肽的产生来鉴定的

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2019-08-27 DOI: 10.1267/ahc.19014

S. Kurabuchi, C. Yao, Gang Chen, K. Hosoi

{"title":"Reversible Conversion among Subtypes of Salivary Gland Duct Cells as Identified by Production of a Variety of Bioactive Polypeptides","authors":"S. Kurabuchi, C. Yao, Gang Chen, K. Hosoi","doi":"10.1267/ahc.19014","DOIUrl":"https://doi.org/10.1267/ahc.19014","url":null,"abstract":"Four major kallikreins (mK1, mK22, mK9, and mK13) were identified in the mouse submandibular gland (SMG). mK1, a true tissue kallikrein, was used as a protein marker to identify different types of SMG granular convoluted tubule (GCT) cells along with epidermal growth factor (EGF), nerve growth factor (NGF), and renin. Kallikrein mK1 was localized in a very small number (~5%) of GCT cells, which were scattered throughout the GCT, indicating that the majority of GCT cells are mK1-negative. Among mK1-positive cells, particularly strong signals were observed in a small number of narrow cells, recognized as slender granular cells (SG cells, Type IV), in the GCT. After postnatal development of the SMG, GCT cells are no longer uniform based on the bioactive substances (mK1, EGF, NGF, and renin) that they produce and secrete. GCT cells were classified into four subtypes, Types I–IV, and it became clear that these subtypes are complicatedly and reversibly converted by the endocrine hormones 5α-dihydrotestosterone (DHT) and triiodothyronine (T3). Duct segments with similar morphology or hormone dependency were recognized in the sublingual and parotid glands. The presence of duct cells with such characteristics is therefore a common feature of the three major salivary glands of rodents.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2019-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1267/ahc.19014","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42656424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

The Effect of Estrogen on Hepatic Fat Accumulation during Early Phase of Liver Regeneration after Partial Hepatectomy in Rats 雌激素对大鼠肝部分切除术后肝再生早期肝脏脂肪积累的影响

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2019-08-27 DOI: 10.1267/ahc.19018

Naparee Srisowanna, N. Choijookhuu, Koichi Yano, Baatarsuren Batmunkh, Makoto Ikenoue, Nguyen Nhat Huynh Mai, Y. Yamaguchi, Y. Hishikawa

{"title":"The Effect of Estrogen on Hepatic Fat Accumulation during Early Phase of Liver Regeneration after Partial Hepatectomy in Rats","authors":"Naparee Srisowanna, N. Choijookhuu, Koichi Yano, Baatarsuren Batmunkh, Makoto Ikenoue, Nguyen Nhat Huynh Mai, Y. Yamaguchi, Y. Hishikawa","doi":"10.1267/ahc.19018","DOIUrl":"https://doi.org/10.1267/ahc.19018","url":null,"abstract":"Fatty liver is common in men and post-menopausal women, suggesting that estrogen may be involved in liver lipid metabolism. The aim of this study is to be clear the role of estrogen and estrogen receptor alpha (ERα) in fat accumulation during liver regeneration using the 70% partial hepatectomy (PHX) model in male, female, ovariectomized (OVX) and E2-treated OVX (OVX-E2) rats. Liver tissues were sampled at 0–48 hr after PHX and fat accumulation, fatty acid translocase (FAT/CD36), sterol regulatory element-binding protein (SREBP1c), peroxisome proliferator-activated receptor α (PPARα), proliferative cell nuclear antigen (PCNA) and ERα were examined by Oil Red O, qRT-PCR and immunohistochemistry, respectively. Hepatic fat accumulation was abundant in female and OVX-E2 compared to male and OVX rats. FAT/CD36 expression was observed in female, OVX and OVX-E2 at 0–12 hr after PHX, but not in male rats. At 0 hr, SREBP1c and PPARα were elevated in female and male rats, respectively, but were decreased after PHX in all rats. The PCNA labeling index reached a maximum at 36 hr and 48 hr in OVX-E2 and OVX rats, respectively. ERα expression in OVX-E2 was higher than OVX at 0–36 hr after PHX. In conclusion, these results indicated that estrogen and ERα might play an important role in fat accumulation related to FAT/CD36 during early phase of rat liver regeneration.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2019-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1267/ahc.19018","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42672386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Modulation of αvβ3 Integrin via Transactivation of β3 Integrin Gene on Murine Bone Marrow Macrophages by 1,25(OH)2D3, Retinoic Acid and Interleukin-4 1,25（OH）2D3、维甲酸和白细胞介素-4反式激活小鼠骨髓巨噬细胞的β3整合素基因调节αvβ3整合素

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2019-08-10 DOI: 10.1267/ahc.19015

S. Kitazawa, Ryuma Haraguchi, Y. Kohara, R. Kitazawa

引用次数: 1