Acta Histochemica Et Cytochemica最新文献_第9页

Comparative Analysis of Immunohistochemical Staining Intensity Determined by Light Microscopy, ImageJ and QuPath in Placental Hofbauer Cells. 胎盘霍夫鲍尔细胞光镜、ImageJ和QuPath免疫组化染色强度的比较分析。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2021-02-25 Epub Date: 2021-02-20 DOI: 10.1267/ahc.20-00032

Katerina Cizkova, Tereza Foltynkova, Mariam Gachechiladze, Zdenek Tauber

{"title":"Comparative Analysis of Immunohistochemical Staining Intensity Determined by Light Microscopy, ImageJ and QuPath in Placental Hofbauer Cells.","authors":"Katerina Cizkova, Tereza Foltynkova, Mariam Gachechiladze, Zdenek Tauber","doi":"10.1267/ahc.20-00032","DOIUrl":"https://doi.org/10.1267/ahc.20-00032","url":null,"abstract":"Software based analyses of immunohistochemical staining are designed for obtaining quantitative, reproducible, and objective data. However, often times only a certain type of positive cells or structures need to be quantified thus whole image analysis cannot be performed. Such an example is Hofbauer placental cells, which show positivity of some antigens together with trophoblast, but only Hofbauer cells represent the regions of interest (ROIs). Two independent observers evaluated the immunohistochemical staining intensity of Hofbauer cells in placenta samples stained for cytoplasmic antigens by ImageJ, QuPath and light microscopy. Thus, the precise manual determination of ROIs, i.e. Hofbauer cells, was necessary. We detected low inter-observer variability in staining intensity. Almost perfect agreement between observers was reached for ImageJ and QuPath whilst substantial agreement was reached for light microscopy evaluation. As for the comparison of ImageJ, QuPath and light microscopy, the agreement of all three methods (identical immunohistochemical intensity) was achieved for 38.1% samples. The almost perfect agreement of staining intensities was reached between ImageJ and QuPath, and moderate agreement for comparison of the light microscopy to both software. Software analyses are much more time-consuming, thus their utilization is at least questionable to evaluate ROIs with selection.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"54 1","pages":"21-29"},"PeriodicalIF":2.4,"publicationDate":"2021-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/17/df/ahc-054-21.PMC7947637.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25488574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

Minocycline Alleviates Cluster Formation of Activated Microglia and Age-dependent Dopaminergic Cell Death in the Substantia Nigra of Zitter Mutant Rat. 米诺环素减轻Zitter突变大鼠黑质活化小胶质细胞簇形成和年龄依赖性多巴胺能细胞死亡。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2020-12-25 Epub Date: 2020-11-21 DOI: 10.1267/ahc.20-00022

Daisuke Taguchi, Ayuka Ehara, Taro Kadowaki, Shin-Ichi Sakakibara, Kazuhiko Nakadate, Koichi Hirata, Shuichi Ueda

{"title":"Minocycline Alleviates Cluster Formation of Activated Microglia and Age-dependent Dopaminergic Cell Death in the Substantia Nigra of Zitter Mutant Rat.","authors":"Daisuke Taguchi, Ayuka Ehara, Taro Kadowaki, Shin-Ichi Sakakibara, Kazuhiko Nakadate, Koichi Hirata, Shuichi Ueda","doi":"10.1267/ahc.20-00022","DOIUrl":"https://doi.org/10.1267/ahc.20-00022","url":null,"abstract":"Microglial activation is a component of neurodegenerative pathology. Here, we examine whether activated microglia participate in age-related dopaminergic (DA) cell death in the substantia nigra pars compacta (SNc) of the zitter (zi/zi) rat, a mutant characterized by deletion of the attractin gene. Confocal microscopy with double-immunohistochemical staining revealed activated microglia-formed cell-clusters surrounding DA neurons in the SNc from 2 weeks after birth. An immunoelectron microscopic study showed that the cytoplasm of activated microglia usually contains phagosome-like vacuoles and lamellar inclusions. Expression levels of the pro-inflammatory cytokines interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and inducible nitric oxide synthase (iNOS) were increased in the midbrain of 2-month-old zi/zi rats. Chronic treatment with the anti-inflammatory agent minocycline altered the morphology of the microglia, reduced cluster formation by the microglia, and attenuated DA cell death in the SNc, and reduced the expression of IL-1β in the midbrain. These results indicate that activated microglia, at least in part and especially at the initial phase, contribute to DA cell death in the SNc of the zi/zi rat.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"53 6","pages":"139-146"},"PeriodicalIF":2.4,"publicationDate":"2020-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7785462/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38812593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Mercury and Selenium Localization in the Cerebrum, Cerebellum, Liver, and Kidney of a Minamata Disease Case. 汞和硒在水俣病患者脑、小脑、肝和肾中的定位。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2020-12-25 Epub Date: 2020-12-19 DOI: 10.1267/ahc.20-00009

Masumi Marumoto, Mineshi Sakamoto, Kohji Marumoto, Shozo Tsuruta, Yoshihiro Komohara

{"title":"Mercury and Selenium Localization in the Cerebrum, Cerebellum, Liver, and Kidney of a Minamata Disease Case.","authors":"Masumi Marumoto, Mineshi Sakamoto, Kohji Marumoto, Shozo Tsuruta, Yoshihiro Komohara","doi":"10.1267/ahc.20-00009","DOIUrl":"https://doi.org/10.1267/ahc.20-00009","url":null,"abstract":"Minamata disease is a methylmercury poisoning caused by consumption of marine food contaminated by man-made methylmercury environmental pollution, and its most prominent feature is marked pathological changes in the central nervous system. Morphological alterations are less pronounced in the liver and the kidney, although their mercury levels are higher than those of the brain. In marine mammals, methylmercury is known to be easily converted to inorganic mercury and it combines with selenium forming mercury selenide, which may counteract the toxicity of mercury. However, little is known about the formation of mercury and selenium complex in human organs. In the present study, we examined the cerebrum, cerebellum, liver, and kidney of a Minamata disease case to study the mercury and selenium localization using electron probe microanalysis. Our results indicated the mercury and selenium localization in the specified tissue of the brain, liver, and kidney such as glial cells, Kupffer cells, and renal tubules.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"53 6","pages":"147-155"},"PeriodicalIF":2.4,"publicationDate":"2020-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7785461/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38812594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

High-quality Fluorescence Imaging of the Human Acrosyringium Using a Transparency: Enhancing Technique and an Improved, Fluorescent Solvatochromic Pyrene Probe. 利用透明增强技术和改进的荧光溶剂致变色芘探针对人肢端注射器进行高质量荧光成像。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2020-12-25 Epub Date: 2020-11-21 DOI: 10.1267/ahc.20-00020

Masamoto Murakami, Ryosuke Kawakami, Yosuke Niko, Teruko Tsuda, Hideki Mori, Kazuki Yatsuzuka, Takeshi Imamura, Koji Sayama

{"title":"High-quality Fluorescence Imaging of the Human Acrosyringium Using a Transparency: Enhancing Technique and an Improved, Fluorescent Solvatochromic Pyrene Probe.","authors":"Masamoto Murakami, Ryosuke Kawakami, Yosuke Niko, Teruko Tsuda, Hideki Mori, Kazuki Yatsuzuka, Takeshi Imamura, Koji Sayama","doi":"10.1267/ahc.20-00020","DOIUrl":"https://doi.org/10.1267/ahc.20-00020","url":null,"abstract":"Two-photon, excitation fluorescent microscopy featuring autofluorescence or immunofluorescence, combined with optical clearance using a transparency-enhancing technique, allows deep imaging of three-dimensional (3D) skin structures. However, it remains difficult to obtain high-quality images of individual cells or 3D structures. We combined a new dye with a transparency-enhancing technology and performed high-quality structural analysis of human epidermal structures, especially the acrosyringium. Human fingertip skin samples were collected, formalin-fixed, embedded in both frozen and paraffin blocks, sliced, stained with propidium iodide, optically cleared using a transparency-enhancing technique, and stained with a new fluorescent, solvatochromic pyrene probe. Microscopy revealed fine skin features and detailed epidermal structures including the stratum corneum (horny layer), keratinocytes, eccrine sweat glands, and peripheral nerves. Three-dimensional reconstruction of an entire acrosyringium was possible in one sample. This new fluorescence microscopy technique yields high-quality epidermal images and will aid in histopathological analyses of skin disorders.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"53 6","pages":"131-138"},"PeriodicalIF":2.4,"publicationDate":"2020-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7785460/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38812592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Loss of Annexin A10 Expression Is Associated with Poor Prognosis in Early Gastric Cancer. 早期胃癌患者Annexin A10表达缺失与预后不良有关

IF 1.6 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2020-10-29 Epub Date: 2020-09-24 DOI: 10.1267/ahc.20-00014

Akira Ishikawa, Kazuya Kuraoka, Junichi Zaitsu, Akihisa Saito, Toshio Kuwai, Takahisa Suzuki, Hirotaka Tashiro, Kiyomi Taniyama, Wataru Yasui

{"title":"Loss of Annexin A10 Expression Is Associated with Poor Prognosis in Early Gastric Cancer.","authors":"Akira Ishikawa, Kazuya Kuraoka, Junichi Zaitsu, Akihisa Saito, Toshio Kuwai, Takahisa Suzuki, Hirotaka Tashiro, Kiyomi Taniyama, Wataru Yasui","doi":"10.1267/ahc.20-00014","DOIUrl":"10.1267/ahc.20-00014","url":null,"abstract":"Gastric cancer (GC) is the third most common cause of cancer-related mortality. The diagnosis and treatment of early GC is a crucial strategy for prognostic improvement of GC. Annexin A10 (ANXA10), a calcium-/phospholipid-binding protein, is a member of the annexin family. The significance of ANXA10 expression in early GC remains unclear. This is the first report to investigate ANXA10 expression in early GC. We performed immunohistochemistry to evaluate ANXA10 expression in early GC, and the correlation between ANXA10 and clinicopathological factors. The loss of ANXA10 expression was detected in 63 (61.2%) of 103 early GC cases and significantly correlated with poor overall survival in patients. Sex, pT stage, pN stage, histology, and ANXA10 expression were associated with poor survival. Sex, histology, and ANXA10 expression were determined as independent predictors of survival in early GC patients. ANXA10 immunostaining could be a new decision-making biomarker in GC.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"53 5","pages":"113-119"},"PeriodicalIF":1.6,"publicationDate":"2020-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7642482/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38591135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Chemokine CXCL14-like Immunoreactivity Co-exists with Somatostatin, but not NPY in the Rat Dorsal Horn and Has Intimate Association with GABAergic Neurons in the Lateral Spinal Nucleus. 趋化因子cxcl14样免疫反应性在大鼠背角与生长抑素共存，而与NPY不共存，并与脊髓外核gaba能神经元密切相关。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2020-10-29 Epub Date: 2020-10-20 DOI: 10.1267/ahc.20-00004

Toshiharu Yamamoto, Kenichi Sasaguri, Natsuki Mizumoto, Hirohumi Suzuki

{"title":"The Chemokine CXCL14-like Immunoreactivity Co-exists with Somatostatin, but not NPY in the Rat Dorsal Horn and Has Intimate Association with GABAergic Neurons in the Lateral Spinal Nucleus.","authors":"Toshiharu Yamamoto, Kenichi Sasaguri, Natsuki Mizumoto, Hirohumi Suzuki","doi":"10.1267/ahc.20-00004","DOIUrl":"https://doi.org/10.1267/ahc.20-00004","url":null,"abstract":"Recent studies have proposed that the chemokine CXCL14 not only has a chemotactic activity, but also functions as a neuromodulator and/or neurotransmitter. In this study, we investigated the distribution of CXCL14 immunoreactive structures in the rat spinal cord and clarified the association of these structures with somatostatin, glutamic acid decarboxylase (GAD; a marker for GABAergic neurons), and neuropeptide Y (NPY). CXCL14 immunoreactive fibers and puncta were observed in lamina II, which modulates somatosensation including nociception, and the lateral spinal nucleus of the spinal dorsal horn at cervical, thoracic, and lumber spinal cord levels. These CXCL14 immunoreactive structures were also immuno-positive for somatostatin, but were immuno-negative for GAD and NPY. In the cervical lateral spinal nucleus, CXCL14 immunoreactive puncta, which were also immuno-positive for somatostatin, existed along the proximal dendrites of some of GABAergic neurons. Together, these results suggest that CXCL14 contributes to the modulation of somatosensation in concert with somatostatin. Neurons targeted by the CXCL14 fiber system include GABAergic neurons located in the lateral spinal nucleus suggesting that CXCL14 with somatostatin can influence the GABAergic neuron function.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"53 5","pages":"121-129"},"PeriodicalIF":2.4,"publicationDate":"2020-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7642483/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38591136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Morphological and Functional Changes in Skin of Adult Male Rats Chronically Treated with Letrozole, a Nonsteroidal Inhibitor of Cytochrome P450 Aromatase. 来曲唑(细胞色素P450芳香化酶的非甾体抑制剂)对成年雄性大鼠皮肤形态学和功能的影响

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2020-10-29 Epub Date: 2020-09-08 DOI: 10.1267/ahc.20009

Kamila Misiakiewicz-Has, Alicja Zawiślak, Anna Pilutin, Agnieszka Kolasa-Wołosiuk, Paweł Szumilas, Ewa Duchnik, Barbara Wiszniewska

{"title":"Morphological and Functional Changes in Skin of Adult Male Rats Chronically Treated with Letrozole, a Nonsteroidal Inhibitor of Cytochrome P450 Aromatase.","authors":"Kamila Misiakiewicz-Has, Alicja Zawiślak, Anna Pilutin, Agnieszka Kolasa-Wołosiuk, Paweł Szumilas, Ewa Duchnik, Barbara Wiszniewska","doi":"10.1267/ahc.20009","DOIUrl":"https://doi.org/10.1267/ahc.20009","url":null,"abstract":"Skin is a target for hormones and a site of hormone production. Aromatase inhibitors such as letrozole reduce circulating estrogen. The aim of the study was to investigate the morphology of the dermis and immunoexpression of androgen receptor (AR), estrogen receptor α and β (ERα, ERβ), luteinizing hormone receptor (LHR), follicle-stimulating hormone receptor (FSHR), and cytochrome P450 aromatase (P450arom) in male rats with a deficit of estradiol. Experiments were performed on skin of 12 male rats. Rats in the experimental group received per os letrozole for 6 months. For morphological analysis, van Gieson, Sirius Red and orcein staining of sections was performed. In immunohistochemistry, reactions with specific antibodies (anti-P450arom, LHR, FSHR, ERα, ERβ) were used. In morphometric analysis, sections were stained with hematoxylin and eosin. Differences between groups were assessed by Mann-Whitney U-test. There were no differences in the diameter of collagen fibers. The dermis of letrozole-treated animals showed areas without collagen fibers, and expression of P450arom, ERα and ERβ was diminished in the skin of these animals. This study indicates that estrogens exert an effect via ERs that has a role in maintaining proper skin morphology in males, together with androgen. This is also the first documented expression of FSHR in the skin of male rats.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"53 5","pages":"99-111"},"PeriodicalIF":2.4,"publicationDate":"2020-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7642481/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38686971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Fatty Acid Binding Protein 7 is Involved in the Proliferation of Reactive Astrocytes, but not in Cell Migration and Polarity. 脂肪酸结合蛋白7参与反应性星形胶质细胞的增殖，但不参与细胞迁移和极性。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2020-08-26 Epub Date: 2020-07-04 DOI: 10.1267/ahc.20001

Tomonori Hara, Banlanjo Abdulaziz Umaru, Kazem Sharifi, Takeo Yoshikawa, Yuji Owada, Yoshiteru Kagawa

{"title":"Fatty Acid Binding Protein 7 is Involved in the Proliferation of Reactive Astrocytes, but not in Cell Migration and Polarity.","authors":"Tomonori Hara, Banlanjo Abdulaziz Umaru, Kazem Sharifi, Takeo Yoshikawa, Yuji Owada, Yoshiteru Kagawa","doi":"10.1267/ahc.20001","DOIUrl":"https://doi.org/10.1267/ahc.20001","url":null,"abstract":"Reactive gliosis is a defense mechanism to minimize and repair the initial damage after CNS injuries that is characterized by increases in astrocytic reactivity and proliferation, with enhanced expression of glial fibrillary acidic protein (GFAP) and cellular hypertrophy. Fatty acid binding protein 7 (FABP7) is abundantly expressed in several types of glial cells, such as astrocytes and oligodendrocyte precursor cells, during brain development and FABP7-positive astrocytes have been shown to be significantly increased in the mouse cortex after a stab injury. However, the functional significance of FABP7 in gliosis remains unclear. In the present study, we examined the mechanism of FABP7-mediated regulation of gliosis using an in vitro scratch-injury model using primary cultured astrocytes. Western blotting showed that FABP7 expression was increased significantly in scratch wounded astrocytes at the edge of the injury compared with intact astrocytes. Through monitoring the occupancy of the injured area, FAB7-KO astrocytes showed a slower proliferation rate compared with WT astrocytes after 48 hr, which was confirmed by BrdU immunostaining. There were no differences in cell migration and polarity of reactive astrocytes between FABP-KO and WT. Conclusively, our data suggest that FABP7 is important in the proliferation of reactive astrocytes in the context of CNS injury.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"53 4","pages":"73-81"},"PeriodicalIF":2.4,"publicationDate":"2020-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7450179/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38334857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Photodynamic Therapy Using a Novel Phosphorus Tetraphenylporphyrin Induces an Anticancer Effect via Bax/Bcl-xL-related Mitochondrial Apoptosis in Biliary Cancer Cells. 新型磷四苯基卟啉光动力疗法通过Bax/ bcl - xl相关线粒体凋亡诱导胆道癌细胞的抗癌作用

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2020-08-26 Epub Date: 2020-07-04 DOI: 10.1267/ahc.20-00002

Nguyen Nhat Huynh Mai, Yuya Yamaguchi, Narantsog Choijookhuu, Jin Matsumoto, Atsushi Nanashima, Hideaki Takagi, Katsuaki Sato, Le Quoc Tuan, Yoshitaka Hishikawa

{"title":"Photodynamic Therapy Using a Novel Phosphorus Tetraphenylporphyrin Induces an Anticancer Effect via Bax/Bcl-xL-related Mitochondrial Apoptosis in Biliary Cancer Cells.","authors":"Nguyen Nhat Huynh Mai, Yuya Yamaguchi, Narantsog Choijookhuu, Jin Matsumoto, Atsushi Nanashima, Hideaki Takagi, Katsuaki Sato, Le Quoc Tuan, Yoshitaka Hishikawa","doi":"10.1267/ahc.20-00002","DOIUrl":"https://doi.org/10.1267/ahc.20-00002","url":null,"abstract":"Photodynamic therapy (PDT) uses photosensitizer activation by light of a specific wavelength, and is a promising treatment for various cancers; however, the detailed mechanism of PDT remains unclear. Therefore, we investigated the anticancer effect of PDT using a novel phosphorus tetraphenylporphyrin (Ptpp) in combination with light emitting diodes (Ptpp-PDT) in the NOZ human biliary cancer cell line. Cell viability and apoptosis were examined by MTT assay, flow cytometry and TUNEL assay for 24 hr after Ptpp-PDT. MitoTracker and JC-1 were used as markers of mitochondrial localization and membrane potential. The levels of mitochondrial oxidative phosphorylation (OXPHOS) complexes, Bcl-2 family proteins, cytochrome c and cleaved caspase-3 were examined by western blotting and immunohistochemistry. The results revealed that Ptpp localized to mitochondria, and that Ptpp-PDT efficiently decreased cell viability in a dose- and time-dependent manner. JC-1 and OXPHOS complexes decreased, but apoptotic cells increased from 6 to 24 hr after Ptpp-PDT. A decrease in Bcl-xL and increases in Bax, cytochrome c and cleaved caspase-3 were also found from 6 to 24 hr after Ptpp-PDT. Based on these results, we conclude that Ptpp-PDT induces anticancer effects via the mitochondrial apoptotic pathway by altering the Bax/Bcl-xL ratio, and could be an effective treatment for human biliary cancer.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"53 4","pages":"61-72"},"PeriodicalIF":2.4,"publicationDate":"2020-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7450180/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38431082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Microhemorrhage in a Rat Model of Neonatal Shaking Brain Injury: Correlation between MRI and Iron Histochemistry. 新生儿摇脑损伤大鼠模型微出血:MRI与铁组织化学的相关性。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2020-08-26 Epub Date: 2020-08-06 DOI: 10.1267/ahc.20007

Daisuke Taguchi, Ayuka Ehara, Yoshiteru Seo, Shuichi Ueda

{"title":"Microhemorrhage in a Rat Model of Neonatal Shaking Brain Injury: Correlation between MRI and Iron Histochemistry.","authors":"Daisuke Taguchi, Ayuka Ehara, Yoshiteru Seo, Shuichi Ueda","doi":"10.1267/ahc.20007","DOIUrl":"https://doi.org/10.1267/ahc.20007","url":null,"abstract":"Previous studies have shown that neonatal shaking brain injury (SBI) causes transient microhemorrhages (MHs) in the gray matter of the cerebral cortex and hippocampus. Iron deposits and iron-uptake cells are observed surrounding MHs in this SBI model, suggesting local hypoxic-ischemic conditions. However, whether the shaken pups suffered systemic hypoxic-ischemic conditions has remained uncertain. Further, histopathological correlations of MHs on magnetic resonance imaging (MRI) are still unclear. The present study examined MHs after neonatal SBI using a combination of histochemical and susceptibility-weighted imaging (SWI) analyses. Systemic oxygen saturation analyses indicated no significant difference between shaken and non-shaken pups. MHs on postnatal day 4 (P4) pups showed decreased signal intensity on SWI. Iron histochemistry revealed that these hypointense areas almost completely comprised red blood cells (RBCs). MHs that appeared on P4 gradually disappeared by P7-12 on SWI. These resolved areas contained small numbers of RBCs, numerous iron-positive cells, and punctate regions with iron reaction products. Perivascular iron products were evident after P12. These changes progressed faster in the hippocampus than in cortical areas. These changes in MHs following neonatal SBI may provide new insights into microvascular pathologies and impacts on brain functions as adults.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"53 4","pages":"83-91"},"PeriodicalIF":2.4,"publicationDate":"2020-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7450178/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38431083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0