Acta Histochemica Et Cytochemica最新文献_第9页

The Chemokine CXCL14-like Immunoreactivity Co-exists with Somatostatin, but not NPY in the Rat Dorsal Horn and Has Intimate Association with GABAergic Neurons in the Lateral Spinal Nucleus. 趋化因子cxcl14样免疫反应性在大鼠背角与生长抑素共存，而与NPY不共存，并与脊髓外核gaba能神经元密切相关。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2020-10-29 Epub Date: 2020-10-20 DOI: 10.1267/ahc.20-00004

Toshiharu Yamamoto, Kenichi Sasaguri, Natsuki Mizumoto, Hirohumi Suzuki

{"title":"The Chemokine CXCL14-like Immunoreactivity Co-exists with Somatostatin, but not NPY in the Rat Dorsal Horn and Has Intimate Association with GABAergic Neurons in the Lateral Spinal Nucleus.","authors":"Toshiharu Yamamoto, Kenichi Sasaguri, Natsuki Mizumoto, Hirohumi Suzuki","doi":"10.1267/ahc.20-00004","DOIUrl":"https://doi.org/10.1267/ahc.20-00004","url":null,"abstract":"Recent studies have proposed that the chemokine CXCL14 not only has a chemotactic activity, but also functions as a neuromodulator and/or neurotransmitter. In this study, we investigated the distribution of CXCL14 immunoreactive structures in the rat spinal cord and clarified the association of these structures with somatostatin, glutamic acid decarboxylase (GAD; a marker for GABAergic neurons), and neuropeptide Y (NPY). CXCL14 immunoreactive fibers and puncta were observed in lamina II, which modulates somatosensation including nociception, and the lateral spinal nucleus of the spinal dorsal horn at cervical, thoracic, and lumber spinal cord levels. These CXCL14 immunoreactive structures were also immuno-positive for somatostatin, but were immuno-negative for GAD and NPY. In the cervical lateral spinal nucleus, CXCL14 immunoreactive puncta, which were also immuno-positive for somatostatin, existed along the proximal dendrites of some of GABAergic neurons. Together, these results suggest that CXCL14 contributes to the modulation of somatosensation in concert with somatostatin. Neurons targeted by the CXCL14 fiber system include GABAergic neurons located in the lateral spinal nucleus suggesting that CXCL14 with somatostatin can influence the GABAergic neuron function.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"53 5","pages":"121-129"},"PeriodicalIF":2.4,"publicationDate":"2020-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7642483/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38591136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Morphological and Functional Changes in Skin of Adult Male Rats Chronically Treated with Letrozole, a Nonsteroidal Inhibitor of Cytochrome P450 Aromatase. 来曲唑(细胞色素P450芳香化酶的非甾体抑制剂)对成年雄性大鼠皮肤形态学和功能的影响

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2020-10-29 Epub Date: 2020-09-08 DOI: 10.1267/ahc.20009

Kamila Misiakiewicz-Has, Alicja Zawiślak, Anna Pilutin, Agnieszka Kolasa-Wołosiuk, Paweł Szumilas, Ewa Duchnik, Barbara Wiszniewska

{"title":"Morphological and Functional Changes in Skin of Adult Male Rats Chronically Treated with Letrozole, a Nonsteroidal Inhibitor of Cytochrome P450 Aromatase.","authors":"Kamila Misiakiewicz-Has, Alicja Zawiślak, Anna Pilutin, Agnieszka Kolasa-Wołosiuk, Paweł Szumilas, Ewa Duchnik, Barbara Wiszniewska","doi":"10.1267/ahc.20009","DOIUrl":"https://doi.org/10.1267/ahc.20009","url":null,"abstract":"Skin is a target for hormones and a site of hormone production. Aromatase inhibitors such as letrozole reduce circulating estrogen. The aim of the study was to investigate the morphology of the dermis and immunoexpression of androgen receptor (AR), estrogen receptor α and β (ERα, ERβ), luteinizing hormone receptor (LHR), follicle-stimulating hormone receptor (FSHR), and cytochrome P450 aromatase (P450arom) in male rats with a deficit of estradiol. Experiments were performed on skin of 12 male rats. Rats in the experimental group received per os letrozole for 6 months. For morphological analysis, van Gieson, Sirius Red and orcein staining of sections was performed. In immunohistochemistry, reactions with specific antibodies (anti-P450arom, LHR, FSHR, ERα, ERβ) were used. In morphometric analysis, sections were stained with hematoxylin and eosin. Differences between groups were assessed by Mann-Whitney U-test. There were no differences in the diameter of collagen fibers. The dermis of letrozole-treated animals showed areas without collagen fibers, and expression of P450arom, ERα and ERβ was diminished in the skin of these animals. This study indicates that estrogens exert an effect via ERs that has a role in maintaining proper skin morphology in males, together with androgen. This is also the first documented expression of FSHR in the skin of male rats.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"53 5","pages":"99-111"},"PeriodicalIF":2.4,"publicationDate":"2020-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7642481/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38686971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Fatty Acid Binding Protein 7 is Involved in the Proliferation of Reactive Astrocytes, but not in Cell Migration and Polarity. 脂肪酸结合蛋白7参与反应性星形胶质细胞的增殖，但不参与细胞迁移和极性。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2020-08-26 Epub Date: 2020-07-04 DOI: 10.1267/ahc.20001

Tomonori Hara, Banlanjo Abdulaziz Umaru, Kazem Sharifi, Takeo Yoshikawa, Yuji Owada, Yoshiteru Kagawa

{"title":"Fatty Acid Binding Protein 7 is Involved in the Proliferation of Reactive Astrocytes, but not in Cell Migration and Polarity.","authors":"Tomonori Hara, Banlanjo Abdulaziz Umaru, Kazem Sharifi, Takeo Yoshikawa, Yuji Owada, Yoshiteru Kagawa","doi":"10.1267/ahc.20001","DOIUrl":"https://doi.org/10.1267/ahc.20001","url":null,"abstract":"Reactive gliosis is a defense mechanism to minimize and repair the initial damage after CNS injuries that is characterized by increases in astrocytic reactivity and proliferation, with enhanced expression of glial fibrillary acidic protein (GFAP) and cellular hypertrophy. Fatty acid binding protein 7 (FABP7) is abundantly expressed in several types of glial cells, such as astrocytes and oligodendrocyte precursor cells, during brain development and FABP7-positive astrocytes have been shown to be significantly increased in the mouse cortex after a stab injury. However, the functional significance of FABP7 in gliosis remains unclear. In the present study, we examined the mechanism of FABP7-mediated regulation of gliosis using an in vitro scratch-injury model using primary cultured astrocytes. Western blotting showed that FABP7 expression was increased significantly in scratch wounded astrocytes at the edge of the injury compared with intact astrocytes. Through monitoring the occupancy of the injured area, FAB7-KO astrocytes showed a slower proliferation rate compared with WT astrocytes after 48 hr, which was confirmed by BrdU immunostaining. There were no differences in cell migration and polarity of reactive astrocytes between FABP-KO and WT. Conclusively, our data suggest that FABP7 is important in the proliferation of reactive astrocytes in the context of CNS injury.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"53 4","pages":"73-81"},"PeriodicalIF":2.4,"publicationDate":"2020-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7450179/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38334857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Photodynamic Therapy Using a Novel Phosphorus Tetraphenylporphyrin Induces an Anticancer Effect via Bax/Bcl-xL-related Mitochondrial Apoptosis in Biliary Cancer Cells. 新型磷四苯基卟啉光动力疗法通过Bax/ bcl - xl相关线粒体凋亡诱导胆道癌细胞的抗癌作用

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2020-08-26 Epub Date: 2020-07-04 DOI: 10.1267/ahc.20-00002

Nguyen Nhat Huynh Mai, Yuya Yamaguchi, Narantsog Choijookhuu, Jin Matsumoto, Atsushi Nanashima, Hideaki Takagi, Katsuaki Sato, Le Quoc Tuan, Yoshitaka Hishikawa

{"title":"Photodynamic Therapy Using a Novel Phosphorus Tetraphenylporphyrin Induces an Anticancer Effect via Bax/Bcl-xL-related Mitochondrial Apoptosis in Biliary Cancer Cells.","authors":"Nguyen Nhat Huynh Mai, Yuya Yamaguchi, Narantsog Choijookhuu, Jin Matsumoto, Atsushi Nanashima, Hideaki Takagi, Katsuaki Sato, Le Quoc Tuan, Yoshitaka Hishikawa","doi":"10.1267/ahc.20-00002","DOIUrl":"https://doi.org/10.1267/ahc.20-00002","url":null,"abstract":"Photodynamic therapy (PDT) uses photosensitizer activation by light of a specific wavelength, and is a promising treatment for various cancers; however, the detailed mechanism of PDT remains unclear. Therefore, we investigated the anticancer effect of PDT using a novel phosphorus tetraphenylporphyrin (Ptpp) in combination with light emitting diodes (Ptpp-PDT) in the NOZ human biliary cancer cell line. Cell viability and apoptosis were examined by MTT assay, flow cytometry and TUNEL assay for 24 hr after Ptpp-PDT. MitoTracker and JC-1 were used as markers of mitochondrial localization and membrane potential. The levels of mitochondrial oxidative phosphorylation (OXPHOS) complexes, Bcl-2 family proteins, cytochrome c and cleaved caspase-3 were examined by western blotting and immunohistochemistry. The results revealed that Ptpp localized to mitochondria, and that Ptpp-PDT efficiently decreased cell viability in a dose- and time-dependent manner. JC-1 and OXPHOS complexes decreased, but apoptotic cells increased from 6 to 24 hr after Ptpp-PDT. A decrease in Bcl-xL and increases in Bax, cytochrome c and cleaved caspase-3 were also found from 6 to 24 hr after Ptpp-PDT. Based on these results, we conclude that Ptpp-PDT induces anticancer effects via the mitochondrial apoptotic pathway by altering the Bax/Bcl-xL ratio, and could be an effective treatment for human biliary cancer.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"53 4","pages":"61-72"},"PeriodicalIF":2.4,"publicationDate":"2020-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7450180/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38431082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Microhemorrhage in a Rat Model of Neonatal Shaking Brain Injury: Correlation between MRI and Iron Histochemistry. 新生儿摇脑损伤大鼠模型微出血:MRI与铁组织化学的相关性。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2020-08-26 Epub Date: 2020-08-06 DOI: 10.1267/ahc.20007

Daisuke Taguchi, Ayuka Ehara, Yoshiteru Seo, Shuichi Ueda

{"title":"Microhemorrhage in a Rat Model of Neonatal Shaking Brain Injury: Correlation between MRI and Iron Histochemistry.","authors":"Daisuke Taguchi, Ayuka Ehara, Yoshiteru Seo, Shuichi Ueda","doi":"10.1267/ahc.20007","DOIUrl":"https://doi.org/10.1267/ahc.20007","url":null,"abstract":"Previous studies have shown that neonatal shaking brain injury (SBI) causes transient microhemorrhages (MHs) in the gray matter of the cerebral cortex and hippocampus. Iron deposits and iron-uptake cells are observed surrounding MHs in this SBI model, suggesting local hypoxic-ischemic conditions. However, whether the shaken pups suffered systemic hypoxic-ischemic conditions has remained uncertain. Further, histopathological correlations of MHs on magnetic resonance imaging (MRI) are still unclear. The present study examined MHs after neonatal SBI using a combination of histochemical and susceptibility-weighted imaging (SWI) analyses. Systemic oxygen saturation analyses indicated no significant difference between shaken and non-shaken pups. MHs on postnatal day 4 (P4) pups showed decreased signal intensity on SWI. Iron histochemistry revealed that these hypointense areas almost completely comprised red blood cells (RBCs). MHs that appeared on P4 gradually disappeared by P7-12 on SWI. These resolved areas contained small numbers of RBCs, numerous iron-positive cells, and punctate regions with iron reaction products. Perivascular iron products were evident after P12. These changes progressed faster in the hippocampus than in cortical areas. These changes in MHs following neonatal SBI may provide new insights into microvascular pathologies and impacts on brain functions as adults.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"53 4","pages":"83-91"},"PeriodicalIF":2.4,"publicationDate":"2020-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7450178/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38431083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rab35 Targeting to the Plasma Membrane Is Dependent on the C-terminal Polybasic Cluster. 靶向质膜的Rab35依赖于c端多碱簇。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2020-08-26 Epub Date: 2020-07-22 DOI: 10.1267/ahc.20-00006

Katsuhisa Kawai, Youhei Egami, Arata Nishigaki, Nobukazu Araki

{"title":"Rab35 Targeting to the Plasma Membrane Is Dependent on the C-terminal Polybasic Cluster.","authors":"Katsuhisa Kawai, Youhei Egami, Arata Nishigaki, Nobukazu Araki","doi":"10.1267/ahc.20-00006","DOIUrl":"https://doi.org/10.1267/ahc.20-00006","url":null,"abstract":"Rab35, a member of the Rab GTPase family, has been implicated in various cellular processes including cell motility and membrane trafficking. Although Rab35 is localized to the plasma membrane, Rab proteins that are identified to have high sequence homology with Rab35 exhibit distinct subcellular localization patterns. Comparing the amino acid sequences between Rab35 and its family members revealed a significant variation in an approximate 30-amino acid region of the C-terminus. This suggests that this region determines the subcellular localization of individual Rab proteins. To confirm this hypothesis, we constructed Rab35-Rab10 chimera proteins by exchanging their C-terminal domains with one another. Confocal microscopy of RAW264 cells expressing EGFP-fused Rab35-Rab10 chimeras has indicated that the C-terminal region of Rab35 is critical for its plasma membrane localization. Furthermore, we were able to determine that a basic amino acid cluster exists in the C-terminal region of Rab35 and that Rab35 localization shifts to the Golgi membrane when the number of basic amino acids in this region is reduced. Thus, it is likely that the approximate 30-amino acid C-terminal region containing basic clusters is responsible for Rab35 plasma membrane localization and that its preferential localization depends on the number of basic amino acids.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"53 4","pages":"93-97"},"PeriodicalIF":2.4,"publicationDate":"2020-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7450177/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38334858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Vitamin D and the Host-Gut Microbiome: A Brief Overview. 维生素 D 与宿主肠道微生物组：简述。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2020-06-26 Epub Date: 2020-06-16 DOI: 10.1267/ahc.20011

Nuraly S Akimbekov, Ilya Digel, Dinara K Sherelkhan, Afzalunnessa B Lutfor, Mohammed S Razzaque

{"title":"Vitamin D and the Host-Gut Microbiome: A Brief Overview.","authors":"Nuraly S Akimbekov, Ilya Digel, Dinara K Sherelkhan, Afzalunnessa B Lutfor, Mohammed S Razzaque","doi":"10.1267/ahc.20011","DOIUrl":"10.1267/ahc.20011","url":null,"abstract":"There is a growing body of evidence for the effects of vitamin D on intestinal host-microbiome interactions related to gut dysbiosis and bowel inflammation. This brief review highlights the potential links between vitamin D and gut health, emphasizing the role of vitamin D in microbiological and immunological mechanisms of inflammatory bowel diseases. A comprehensive literature search was carried out in PubMed and Google Scholar using combinations of keywords \"vitamin D,\" \"intestines,\" \"gut microflora,\" \"bowel inflammation\". Only articles published in English and related to the study topic are included in the review. We discuss how vitamin D (a) modulates intestinal microbiome function, (b) controls antimicrobial peptide expression, and (c) has a protective effect on epithelial barriers in the gut mucosa. Vitamin D and its nuclear receptor (VDR) regulate intestinal barrier integrity, and control innate and adaptive immunity in the gut. Metabolites from the gut microbiota may also regulate expression of VDR, while vitamin D may influence the gut microbiota and exert anti-inflammatory and immune-modulating effects. The underlying mechanism of vitamin D in the pathogenesis of bowel diseases is not fully understood, but maintaining an optimal vitamin D status appears to be beneficial for gut health. Future studies will shed light on the molecular mechanisms through which vitamin D and VDR interactions affect intestinal mucosal immunity, pathogen invasion, symbiont colonization, and antimicrobial peptide expression.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"53 3","pages":"33-42"},"PeriodicalIF":2.4,"publicationDate":"2020-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7322162/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38120568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential Localization and Invasion of Tumor Cells in Mouse Models of Human and Murine Leukemias. 人和小鼠白血病小鼠模型中肿瘤细胞的差异定位和侵袭。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2020-06-26 Epub Date: 2020-04-29 DOI: 10.1267/ahc.19035

Kiyomi Mashima, Morio Azuma, Ken Fujiwara, Takashi Inagaki, Iekuni Oh, Takashi Ikeda, Kento Umino, Hirofumi Nakano, Kaoru Morita, Kazuya Sato, Daisuke Minakata, Ryoko Yamasaki, Masahiro Ashizawa, Chihiro Yamamoto, Shin-Ichiro Fujiwara, Kaoru Hatano, Ken Ohmine, Kazuo Muroi, Nobuhiko Ohno, Yoshinobu Kanda

{"title":"Differential Localization and Invasion of Tumor Cells in Mouse Models of Human and Murine Leukemias.","authors":"Kiyomi Mashima, Morio Azuma, Ken Fujiwara, Takashi Inagaki, Iekuni Oh, Takashi Ikeda, Kento Umino, Hirofumi Nakano, Kaoru Morita, Kazuya Sato, Daisuke Minakata, Ryoko Yamasaki, Masahiro Ashizawa, Chihiro Yamamoto, Shin-Ichiro Fujiwara, Kaoru Hatano, Ken Ohmine, Kazuo Muroi, Nobuhiko Ohno, Yoshinobu Kanda","doi":"10.1267/ahc.19035","DOIUrl":"https://doi.org/10.1267/ahc.19035","url":null,"abstract":"Leukemias are refractory hematopoietic malignancies, for which the development of new therapeutic agents requires in vivo studies using tumor-bearing mouse models. Although several organs are commonly examined in such studies to evaluate the disease course, the effectiveness of interventions and the localization of tumor cells in the affected organs are still unclear. In this study, we histologically examined the distribution of leukemia cells in several organs using two leukemic mouse models produced by the administration of two cell lines (THP-1, a human myelomonocytic leukemia, and A20, a mouse B cell leukemia/lymphoma) to severe immunodeficient mice. Survival of the mice depended on the tumor burden. Although A20 and THP-1 tumor cells massively infiltrated the parenchyma of the liver and spleen at 21 days after transplantation, A20 cells were hardly found in connective tissues in Glisson's capsule in the liver as compared with THP-1 cells. In the bone marrow, there was more severe infiltration of A20 cells than THP-1 cells. THP-1 and A20 cells were widely spread in the lungs, but were rarely observed in the small intestine. These findings suggest that each leukemia model has a unique localization of tumor cells in several affected organs, which could critically affect the disease course and the efficacy of therapeutic agents, including cellular immunotherapies.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"53 3","pages":"43-53"},"PeriodicalIF":2.4,"publicationDate":"2020-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7322163/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38120569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Immunohistochemical Pharmacokinetics of the Anti-diabetes Drug Alogliptin in Rat Kidney and Liver. 大鼠肾脏和肝脏中抗糖尿病药物阿格列汀的免疫组织化学药代动力学

IF 1.6 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2020-06-26 Epub Date: 2020-05-29 DOI: 10.1267/ahc.19036

Yutaro Yamamoto, Yuta Yamamoto, Tetsuya Saita, Daisuke Hira, Takahito Chijiwa, Masashi Shin

{"title":"Immunohistochemical Pharmacokinetics of the Anti-diabetes Drug Alogliptin in Rat Kidney and Liver.","authors":"Yutaro Yamamoto, Yuta Yamamoto, Tetsuya Saita, Daisuke Hira, Takahito Chijiwa, Masashi Shin","doi":"10.1267/ahc.19036","DOIUrl":"10.1267/ahc.19036","url":null,"abstract":"Alogliptin is one of a new class of therapeutic agents for type 2 diabetes called dipeptidyl peptidase-4 inhibitors. Here, we used immunohistochemistry to investigate the pharmacokinetics of alogliptin at the cell and tissue levels in the rat kidney and liver. One hour after alogliptin administration, the most noticeable immunoreactivity in the kidney was a moderate-to-strong staining in proximal tubule S3 segment epithelial cells. On the other hand, immunostaining was found only in the microvilli of S1 and S2 segment cells. Immunoreactivity was also observed in the glomerulus and distal tubules. Positive cells and almost negative cells coexisted in the collecting ducts. Twenty-four hours after administration, moderate immunostaining remained in the S3 segment but staining in other regions had almost disappeared. In the liver 1 hr after administration, hepatocyte staining differed in the hepatic lobule, with zone III being stronger than zone I. Immunostaining had almost disappeared 24 hr after administration. These findings suggest that alogliptin reabsorption at the kidney and uptake at the hepatocyte vary from region to region and that one or more types of transporter are involved in these processes. In addition, long-term alogliptin use may cause the drug to accumulate in S3 segment, leading to adverse events.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"53 3","pages":"55-60"},"PeriodicalIF":1.6,"publicationDate":"2020-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7322161/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38118483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of Novel c-Kit-expressing Smooth Muscle Cells in Murine Cecum. 新型表达c- kit的小鼠盲肠平滑肌细胞的研究。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2020-04-28 Epub Date: 2020-04-09 DOI: 10.1267/ahc.20003

Satoshi Iino, Kazuhide Horiguchi, Satomi Horiguchi

引用次数: 1